Last updated: 2025-03-13 429297 annotations 4738 curated publications
Publication: A single cyclin-CDK complex is sufficient for both mitotic and meiotic progression in fission yeast.
Authors
Gutiérrez-Escribano P, Nurse P
Added to PomBase on 2018-03-26
Citation
Nat Commun 2015 Apr 20;6:6871
ID
PMID:25891897
Links
Abstract
The dominant model for eukaryotic cell cycle control proposes that cell cycle progression is driven by a succession of CDK complexes with different substrate specificities. However, in fission yeast it has been shown that a single CDK complex generated by the fusion of the Cdc13 cyclin with the CDK protein Cdc2 can drive the mitotic cell cycle. Meiosis is a modified cell cycle programme in which a single S-phase is followed by two consecutive rounds of chromosome segregation. Here we systematically analyse the requirements of the different fission yeast cyclins for meiotic cell cycle progression. We also show that a single Cdc13-Cdc2 complex, in the absence of the other cyclins, can drive the meiotic cell cycle. We propose that qualitatively different CDK complexes are not absolutely required for cell cycle progression either during mitosis or meiosis, and that a single CDK complex can drive both cell cycle programmes.
Annotations from this publication:
GO biological process
Single locus phenotype
Cell
Filters: Term
+ abnormal negative regulation of premeiotic DNA replication initiation resulting in complete rereplication
cdc13+[Knockdown]
+ abolished meiosis I
cdc13+[Knockdown]
+ abolished meiosis II
cdc13+[Knockdown]
+ inviable spore
cig2Δ
Multi-locus phenotype
Population
Filters: Term
+ decreased spore germination frequency
cig1Δ cig2Δ puc1Δ
Cell
Filters: Term
+ abnormal spindle pole body morphology during meiosis II
crs1Δ rem1Δ
+ abnormal sporulation resulting in formation of ascus with fewer than four spores
cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cig1Δ cig2Δ puc1Δ rem1Δ
+ abnormal sporulation resulting in formation of ascus with more than four spores
cdc2-cdc13[Overexpression] cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cig1Δ cig2Δ crs1Δ puc1Δ ,   cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cig1Δ cig2Δ puc1Δ rem1Δ
+ abnormal sporulation resulting in formation of ascus with single large spore
cig1Δ cig2Δ crs1Δ puc1Δ
+ abnormal sporulation resulting in formation of azygotic ascus with more than four spores
crs1Δ rem1Δ
+ abnormal sporulation resulting in formation of two-spore ascus
cdc13Δ cdc2-cdc13 cdc2Δ ,   cdc2-cdc13 cig1Δ cig2Δ puc1Δ ,   cig1Δ cig2Δ crs1Δ puc1Δ
+ abolished azygotic meiosis II
cig1Δ cig2Δ puc1Δ
+ abolished premeiotic DNA replication during azygotic meiotic cell cycle
cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cig1Δ cig2Δ puc1Δ rem1Δ
+ abolished zygotic meiosis II
cig1Δ cig2Δ puc1Δ
+ decreased meiotic recombination during zygotic meiosis
cig1Δ cig2Δ puc1Δ
+ delayed onset of premeiotic DNA replication during azygotic meiosis
cig1Δ cig2Δ puc1Δ
+ inviable spore
cdc13Δ cdc2-cdc13 cdc2Δ ,   cdc2-cdc13 cig1Δ cig2Δ puc1Δ ,   cdc2-cdc13[Overexpression] cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cdc2-cdc13[Overexpression] cig1Δ cig2Δ puc1Δ ,   cig1Δ cig2Δ ,   cig1Δ cig2Δ crs1Δ puc1Δ ,   cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cig1Δ cig2Δ puc1Δ ,   cig1Δ cig2Δ puc1Δ rem1Δ
+ normal meiosis
cdc2-cdc13[Overexpression] cig1Δ cig2Δ crs1Δ puc1Δ rem1Δ ,   cdc2-cdc13[Overexpression] cig1Δ cig2Δ puc1Δ rem1Δ ,   crs1Δ rem1Δ
+ normal meiosis I
cdc2-cdc13[Overexpression] cig1Δ cig2Δ puc1Δ
Genetic interaction
Source: Interactions curated by BioGRID and PomBase
Filters: Throughput Interaction Type
Gene Product Interacting gene Interacting product Interaction type
cig1 G1/S-specific B-type cyclin Cig1 phenotype enhanced by crs1 meiosis specific cyclin Crs1 Phenotypic Enhancement
cig1 G1/S-specific B-type cyclin Cig1 phenotype enhanced by puc1 G1 cyclin Puc1 Phenotypic Enhancement
cig2 G1/S-specific B-type cyclin Cig2 phenotype enhanced by crs1 meiosis specific cyclin Crs1 Phenotypic Enhancement
cig2 G1/S-specific B-type cyclin Cig2 phenotype enhanced by puc1 G1 cyclin Puc1 Phenotypic Enhancement
puc1 G1 cyclin Puc1 phenotype enhanced by crs1 meiosis specific cyclin Crs1 Phenotypic Enhancement
rem1 meiosis specific cyclin Rem1 phenotype enhanced by crs1 meiosis specific cyclin Crs1 Phenotypic Enhancement
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