SPAC1002.01,VIABLE WT cells at 25-32 SPAC1002.02,VIABLE WT cells at 25-32 SPAC1002.03c,VIABLE WT cells at 25-32 SPAC1002.04c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC1002.05c,VIABLE WT cells at 25-32 SPAC1002.06c,VIABLE WT cells at 25-32 SPAC1002.07c,VIABLE WT cells at 25-32 SPAC1002.08c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC1002.09c,ESSENTIAL microcolonies misshapen cells at 32- slightlylarger colonies misshapen cells at 25 SPAC1002.10c,ESSENTIAL germinated spores long at 25-32 SPAC1002.11,ESSENTIAL germinated spores at 25-32 SPAC1002.12c,VIABLE WT cells at 25-32 SPAC1002.13c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC1002.14,VIABLE WT cells at 25-32 SPAC1002.15c,VIABLE slightly curved- stubby septated cells at 25-32- cells misshapen at 36 SPAC1002.16c,ESSENTIAL germinated spores- occasionally long and then divide at 25- 32 SPAC1002.17c,VIABLE WT cells at 25-32 SPAC1002.18,VIABLE WT cells at 25-32 SPAC1002.19,VIABLE WT cells at 25-32 SPAC1002.20,VIABLE WT cells at 25-32 SPAC1006.01,VIABLE WT cells at 25-32 SPAC1006.02,ESSENTIAL microcolonies small cells at 25-32 SPAC1006.03c,VIABLE slightly misshapen cells at 32- VIABLE/ESSENTIAL microcolonies misshapen cells at 25 SPAC1006.04c,VIABLE WT cells at 25-32 SPAC1006.05c,VIABLE very small colonies rounded cells at 25-32 SPAC1006.06,VIABLE WT cells at 25-32 SPAC1006.07,ESSENTIAL germinated spores misshapen at 25-32 SPAC1006.08,ESSENTIAL germinated spores long- lysis at 25-32 SPAC1006.09,VIABLE WT cells at 25-32 SPAC1039.01,VIABLE WT cells at 25-32 SPAC1039.02,VIABLE WT cells at 25-32 SPAC1039.03,VIABLE WT cells at 25-32 SPAC1039.04,VIABLE WT cells at 25-32 SPAC1039.05c,VIABLE WT cells at 25-32 SPAC1039.06,VIABLE WT cells at 25-32 SPAC1039.07c,VIABLE WT cells at 25-32 SPAC1039.08,VIABLE WT cells at 25-32 SPAC1039.09,VIABLE WT cells at 25-32 SPAC1039.10,VIABLE WT cells at 25-32 SPAC1039.11c,VIABLE slightly stubby cells at 25-32 SPAC105.01c,VIABLE slightly misshapen cells at 25-32 SPAC105.02c,VIABLE WT cells at 25-32 SPAC105.03c,VIABLE WT cells at 25-32 SPAC1071.01c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC1071.02,VIABLE slightly misshapen cells at 25- small colonies misshapen cells at 32 some cells curved- branched at 36 SPAC1071.03c,VIABLE WT cells at 25-32 SPAC1071.04c,VIABLE stubby cells at 25-32- cells not growing at 36 SPAC1071.05,VIABLE WT cells at 25-32 SPAC1071.06,VIABLE WT cells at 25-32 SPAC1071.07c,VIABLE WT cells at 25-32 SPAC1071.08,VIABLE WT cells at 25-32 SPAC1071.09c,VIABLE WT cells at 25-32 SPAC1071.10c,ESSENTIAL spores- germinated spores at 25-32 SPAC1071.11,VIABLE WT cells at 25-32 SPAC1071.12c,VIABLE WT cells at 25-32 SPAC1093.01,VIABLE WT cells at 25-32 SPAC1093.02,VIABLE WT cells at 25-32 SPAC1093.03,VIABLE WT cells at 25-32 SPAC1093.04c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC1093.05,ESSENTIAL germinated spores slightly misshapen- occasionally divide at 25-32 SPAC1093.06c,VIABLE WT cells at 25-32 SPAC10F6.01c,VIABLE small colonies slightly misshapen cells at 25-32 SPAC10F6.02c,ESSENTIAL spores at 25-32 SPAC10F6.03c,ESSENTIAL spores at 25-32 SPAC10F6.04,VIABLE slightly misshapen cells at 25-32 SPAC10F6.05c,VIABLE WT cells at 25-32 SPAC10F6.06,VIABLE WT cells at 25-32 SPAC10F6.07c,VIABLE WT cells at 25-32 SPAC10F6.08c,VIABLE slightly curved cells at 25-32 SPAC10F6.09c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC10F6.10,ESSENTIAL germinated spores at 25-32 SPAC10F6.11c,VIABLE WT cells at 25-32 SPAC10F6.12c,VIABLE WT cells at 25-32 SPAC10F6.13c,VIABLE very small colonies WT cells at 25-32 SPAC10F6.14c,VIABLE WT cells at 25-32 SPAC10F6.15,VIABLE WT cells at 25-32 SPAC10F6.16,VIABLE WT cells at 25-32 SPAC10F6.17c,VIABLE WT cells at 32 SPAC110.01,VIABLE WT cells at 25-32 SPAC110.02,VIABLE WT cells at 25-32 SPAC110.03,ESSENTIAL spores- germinated spores- microcolonies misshapen cells at 25-32 SPAC110.04c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC1142.01,VIABLE WT cells at 25-32 SPAC1142.02c,VIABLE WT cells at 25-32 SPAC1142.03c,VIABLE WT cells at 25-32 SPAC1142.04,ESSENTIAL spores at 25-32 SPAC1142.05,VIABLE small colonies WT cells at 25-32 SPAC1142.06,VIABLE WT cells at 25-32 SPAC1142.07c,VIABLE small colonies rounded cells at 25-32 SPAC1142.08,VIABLE WT cells at 25-32 SPAC11D3.01c,VIABLE WT cells at 25-32 SPAC11D3.02c,VIABLE WT cells at 25-32 SPAC11D3.03c,VIABLE WT cells at 25-32 SPAC11D3.04c,VIABLE WT cells at 25-32 SPAC11D3.05,VIABLE WT cells at 25-32 SPAC11D3.06,VIABLE WT cells at 25-32 SPAC11D3.07c,VIABLE WT cells at 25-32 SPAC11D3.08c,VIABLE small colonies WT cells at 25-32 SPAC11D3.09,VIABLE WT cells at 25-32 SPAC11D3.10,VIABLE WT cells at 25-32 SPAC11D3.13,VIABLE WT cells at 25-32 SPAC11D3.14c,VIABLE WT cells at 25-32 SPAC11D3.15,VIABLE WT cells at 25-32 SPAC11D3.16c,VIABLE WT cells at 25-32 SPAC11D3.17,VIABLE WT cells at 25-32 SPAC11D3.18c,VIABLE WT cells at 25-32 SPAC11E3.01c,VIABLE slightly curved cells at 25-32 SPAC11E3.02c,ESSENTIAL microcolonies slightly misshapen vacuolated cells at 25-32 SPAC11E3.03,VIABLE small colonies slightly misshapen cells at 25-32 SPAC11E3.04c,VIABLE WT cells at 25-32 SPAC11E3.05,VIABLE very small colonies slightly misshapen cells at 25-32- cells longer at 36 SPAC11E3.06,VIABLE WT cells at 32 SPAC11E3.07,ESSENTIAL spores- germinated spores at 25-32 SPAC11E3.08c,VIABLE long cells at 25-32- cells longer at 36 SPAC11E3.09,VIABLE WT cells at 25-32 SPAC11E3.10,VIABLE WT cells at 25-32 SPAC11E3.11c,VIABLE WT cells at 25-32 SPAC11E3.12,VIABLE WT cells at 25-32 SPAC11E3.13c,VIABLE WT cells at 25-32 SPAC11E3.14,VIABLE WT cells at 25-32 SPAC11E3.15,VIABLE WT cells at 25-32 SPAC11G7.01,VIABLE WT cells at 25-32 SPAC11G7.02,VIABLE stubby cells at 25-32 SPAC11G7.03,VIABLE WT cells at 25-32 SPAC11G7.04,VIABLE small colonies slightly misshapen cells at 25-32 SPAC11G7.05c,VIABLE WT cells at 25-32 SPAC11G7.06c,VIABLE WT cells at 25-32 SPAC11H11.01,VIABLE WT cells at 25-32 SPAC11H11.02c,VIABLE WT cells at 25-32 SPAC11H11.03c,VIABLE WT cells at 25-32 SPAC11H11.04,VIABLE WT cells at 25-32 SPAC11H11.05c,VIABLE WT cells at 25-32 SPAC11H11.06,ESSENTIAL spores- germinated spores misshapen at 25-32 SPAC1250.01,ESSENTIAL microcolonies long curved cells at 25-32 SPAC1250.02,VIABLE WT cells at 25-32 SPAC1250.03,VIABLE WT cells at 25-32 SPAC1250.04c,VIABLE WT cells at 25-32 SPAC1250.05,VIABLE WT cells at 25-32 SPAC1296.01c,VIABLE WT cells at 25-32 SPAC1296.02,VIABLE WT cells at 25-32 SPAC1296.03c,VIABLE WT cells at 25-32 SPAC1296.04,VIABLE WT cells at 25-32 SPAC1296.05c,VIABLE WT cells at 25-32 SPAC1296.06,VIABLE WT cells at 32 SPAC12B10.01c,VIABLE WT cells at 25-32 SPAC12B10.02c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC12B10.03,VIABLE WT cells at 25-32 SPAC12B10.04,VIABLE WT cells at 25-32 SPAC12B10.05,VIABLE WT cells at 25-32 SPAC12B10.06c,VIABLE WT cells at 25-32 SPAC12B10.07,VIABLE WT cells at 25-32 SPAC12B10.08c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC12B10.09,VIABLE WT cells at 25-32 SPAC12B10.10,VIABLE WT cells at 25-32 SPAC12B10.11,VIABLE WT cells at 25-32 SPAC12B10.12c,VIABLE long cells at 32- WT cells at 25 SPAC12B10.13,VIABLE WT cells at 25-32 SPAC12B10.14c,VIABLE small colonies WT cells at 25-32 SPAC12B10.15c,VIABLE WT cells at 25-32 SPAC12B10.16c,VIABLE WT cells at 25-32 SPAC12G12.01c,VIABLE WT cells at 25-32 SPAC12G12.02,ESSENTIAL spores- germinated spores at 25-32 SPAC12G12.03,VIABLE WT cells at 25-32 SPAC12G12.04,ESSENTIAL microcolonies rounded cells at 25-32 SPAC12G12.05c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC12G12.06c,ESSENTIAL germinated spores long at 25-32- often divide at 32 SPAC12G12.07c,VIABLE WT cells at 25-32 SPAC12G12.08,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC12G12.09,VIABLE WT cells at 25-32 SPAC12G12.10,VIABLE WT cells at 25-32 SPAC12G12.11c,VIABLE WT cells at 25-32 SPAC12G12.12,VIABLE WT cells at 25-32 SPAC12G12.13c,VIABLE small colonies slightly long cells at 25-32 SPAC12G12.14c,ESSENTIAL microcolonies swollen- misshapen cells at 25-32 SPAC12G12.15,VIABLE WT cells at 25-32 SPAC12G12.16c,VIABLE WT cells at 25-32 SPAC1327.01c,VIABLE WT cells at 25-32 SPAC139.01c,VIABLE WT cells at 25-32 SPAC139.02c,VIABLE WT cells at 25-32 SPAC139.03,VIABLE WT cells at 25-32 SPAC139.04c,VIABLE WT cells at 25-32 SPAC139.05,VIABLE WT cells at 25-32 SPAC139.06,VIABLE WT cells at 25-32 SPAC1399.01c,VIABLE WT cells at 25-32 SPAC1399.02,VIABLE WT cells at 25-32 SPAC1399.03,VIABLE WT cells at 25-32 SPAC1399.04c,VIABLE WT cells at 25-32 SPAC1399.05c,VIABLE WT cells at 25-32 SPAC13A11.01c,VIABLE stubby cells at 25-32 SPAC13A11.02c,ESSENTIAL germinated spores- occasionally microcolonies misshapen cells at 25-32 SPAC13A11.03,VIABLE WT cells at 25-32- poor germination SPAC13A11.04c,VIABLE WT cells at 25-32 SPAC13A11.05,VIABLE WT cells at 25-32 SPAC13A11.06,VIABLE WT cells at 25-32 SPAC13C5.01c,VIABLE rounded cells at 25-32- better growth at 25 SPAC13C5.02,VIABLE small colonies long misshapen cells at 25-32- cells grow better at 25 SPAC13C5.03,VIABLE WT cells at 25-32 SPAC13C5.04,VIABLE WT cells at 25-32 SPAC13C5.05c,VIABLE WT cells at 25-32 SPAC13C5.06c,VIABLE WT cells at 25-32 SPAC13C5.07,VIABLE small colonies long cells at 25-32 SPAC13D6.01,VIABLE WT cells at 25-32 SPAC13D6.02c,VIABLE WT cells at 25-32 SPAC13D6.03c,VIABLE WT cells at 25-32 SPAC13D6.04c,VIABLE WT cells at 25-32 SPAC13D6.05,ESSENTIAL microcolonies curved cells- often long branched at 32- more division at 25 SPAC13F5.01c,VIABLE long cells at 25-32 SPAC13F5.02c,ESSENTIAL spores- some germinated spores at 25-32 SPAC13F5.03c,VIABLE WT cells at 25-32 SPAC13F5.04c,VIABLE WT cells at 25-32 SPAC13F5.05,VIABLE WT cells at 25-32 SPAC13F5.06c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPAC13F5.07c,VIABLE WT cells at 25-32 SPAC13G6.01c,VIABLE WT cells at 25-32 SPAC13G6.02c,VIABLE WT cells at 25-32 SPAC13G6.03,VIABLE WT cells at 25-32 SPAC13G6.04,VIABLE WT cells at 25-32 SPAC13G6.05c,ESSENTIAL microcolonies long cells at 32- ESSENTIAL/VIABLE small colonies long misshapen cells at 25 SPAC13G6.06c,VIABLE WT cells at 25-32 SPAC13G6.07c,VIABLE WT cells at 25-32 SPAC13G6.08,VIABLE WT cells at 25-32 SPAC13G6.09,VIABLE small colonies WT cells at 25-32 SPAC13G6.10c,VIABLE small colonies stubby cells at 25-32 SPAC13G6.11c,ESSENTIAL spores- germinated spores slightly misshapen may divide once at 25-32 SPAC13G6.12c,VIABLE WT cells at 25-32 SPAC13G6.13,VIABLE WT cells at 25-32 SPAC13G6.14,VIABLE WT cells at 25-32 SPAC13G6.15c,VIABLE WT cells at 25-32 SPAC13G7.01c,ESSENTIAL spores- germinated spores- occasionally microcolonies misshapen cells at 25-32 SPAC13G7.02c,VIABLE WT cells at 25-32 SPAC13G7.03,VIABLE WT cells at 25-32 SPAC13G7.04c,VIABLE WT cells at 25-32 SPAC13G7.05,VIABLE WT cells at 25-32 SPAC13G7.06,VIABLE WT cells at 25-32 SPAC13G7.07,VIABLE WT cells at 25-32 SPAC13G7.08c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC13G7.09c,VIABLE WT cells at 25-32 SPAC13G7.10,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC13G7.11,VIABLE WT cells at 32 SPAC13G7.12c,VIABLE WT cells at 32 SPAC13G7.13c,VIABLE WT cells at 25-32 SPAC140.01,VIABLE WT cells at 25-32 SPAC140.02,VIABLE small colonies WT cells at 25-32 SPAC140.03,VIABLE WT cells at 25-32 SPAC140.04,VIABLE WT cells at 25-32 SPAC1420.01c,VIABLE WT cells at 25-32 SPAC1420.02c,ESSENTIAL spores at 25-32 SPAC1420.03,VIABLE WT cells at 25-32 SPAC1420.04c,VIABLE WT cells at 25-32 SPAC144.01,VIABLE WT cells at 25-32 SPAC144.02,VIABLE curved slightly long cells at 25-32 SPAC144.03,VIABLE WT cells at 25-32 SPAC144.04c,VIABLE WT cells at 25-32 SPAC144.05,VIABLE WT cells at 25-32 SPAC144.06,VIABLE small colonies WT cells at 25-32 SPAC144.07c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPAC144.08,ESSENTIAL microcolonies skittle slightly misshapen cells at 25-32 SPAC144.09c,ESSENTIAL microcolonies misshapen cells at 32- germinated spores at 25 SPAC144.10c,ESSENTIAL germinated spores- often divide once to give one slightly misshapen cell and one dead cell at 25- 32 SPAC144.11,VIABLE slightly long cells at 25-32 SPAC144.12,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPAC144.13c,VIABLE WT cells at 25-32 SPAC144.14,VIABLE WT cells at 25-32 SPAC144.15c,ESSENTIAL germinated spores at 25-32 SPAC144.16,ESSENTIAL germinated spores at 25-32 SPAC144.17c,VIABLE WT cells at 25-32 SPAC144.18,ESSENTIAL microcolonies rounded cells- lysis at 25-32 SPAC1486.01,VIABLE WT cells at 25-32 SPAC1486.02c,VIABLE WT cells at 25-32 SPAC1486.03c,ESSENTIAL germinated spores long at 25-32 SPAC1486.04c,VIABLE small colonies slightly curved- slightly curved cells at 25-32 SPAC1486.05,ESSENTIAL germinated spores misshapen at 25-32 SPAC1486.06,ESSENTIAL spores at 25-32 SPAC1486.07c,ESSENTIAL small colonies skittle cells at 25-32 SPAC1486.08,VIABLE WT cells at 25-32 SPAC1486.09,ESSENTIAL microcolonies long cells at 25-32 SPAC1486.10,VIABLE WT cells at 25-32 SPAC14C4.01c,VIABLE WT cells at 25-32 SPAC14C4.02c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC14C4.03,VIABLE WT cells at 25-32 SPAC14C4.04,VIABLE WT cells at 25-32 SPAC14C4.05c,VIABLE WT cells at 25-32 SPAC14C4.06c,VIABLE small colonies WT cells at 25-32 SPAC14C4.07,VIABLE WT cells at 25-32 SPAC14C4.08,VIABLE WT cells at 25-32 SPAC14C4.09,VIABLE slightly misshapen- septated cells- at 25-32 SPAC14C4.10c,VIABLE WT cells at 25-32 SPAC14C4.11,VIABLE WT cells at 25-32 SPAC14C4.12c,VIABLE WT cells at 25-32 SPAC14C4.13,VIABLE WT cells at 25-32 SPAC14C4.14,VIABLE WT cells at 25-32 SPAC14C4.15c,VIABLE WT cells at 25-32 SPAC14C4.16,VIABLE slightly misshapen cells at 25-32- cells more misshapen after several divisions SPAC1527.01,VIABLE WT cells at 25-32 SPAC1527.02,VIABLE stubby cells at 25-32 SPAC1527.03,VIABLE WT cells at 25-32 SPAC1556.01c,VIABLE small colonies long cells at 25-32 SPAC1556.02c,VIABLE WT cells at 25-32 SPAC1556.03,VIABLE WT cells at 25-32 SPAC1556.04c,VIABLE WT cells at 25-32 SPAC1556.05c,VIABLE slightly curved- slightly vacuolated cells at 32 SPAC1556.06.1,VIABLE WT cells at 25-32 SPAC1556.07,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC1556.08c,VIABLE WT cells at 25-32 SPAC1565.01,VIABLE WT cells at 25-32 SPAC1565.02c,VIABLE WT cells at 25-32 SPAC1565.03,VIABLE WT cells at 25-32 SPAC1565.04c,VIABLE WT cells at 25-32 SPAC1565.05,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPAC1565.06c,ESSENTIAL germinated spores long- lysis at 25-32 SPAC1565.07c,VIABLE WT cells with some long cells at 25-32 SPAC1565.08,ESSENTIAL germinated spores at 25-32 SPAC15A10.01,ESSENTIAL small colonies rounded cells at 25-32 SPAC15A10.02,ESSENTIAL spores- germinated spores at 25-32 SPAC15A10.03c,VIABLE very small colonies long cells at 25-32 SPAC15A10.04c,ESSENTIAL spores- germinated spores- occasionally microcolonies misshapen cells at 25-32 SPAC15A10.05c,VIABLE WT cells at 25-32 SPAC15A10.06,VIABLE WT cells at 32 cells slightly misshapen at 25 SPAC15A10.07,VIABLE WT cells at 25-32 SPAC15A10.08,VIABLE WT cells at 25-32 SPAC15A10.09c,VIABLE WT cells at 25-32 SPAC15A10.10,VIABLE WT cells at 25-32 SPAC15A10.11,VIABLE WT cells at 25-32 SPAC15A10.12c,ESSENTIAL small colonies slightly long misshapen cells- at 25- cells longer at 32 SPAC15A10.13,VIABLE WT cells at 25-32 SPAC15A10.15,VIABLE WT cells at 25-32 SPAC15A10.16,VIABLE WT cells at 25-32 SPAC15E1.02c,VIABLE WT cells at 25-32 SPAC15E1.04,VIABLE slightly misshapen cells at 25-32- ESSENTIAL microcolonies misshapen cells at 36. SPAC15E1.05c,VIABLE WT cells with some long cells at 25-32 SPAC15E1.06,VIABLE WT cells at 25-32 SPAC15E1.07c,VIABLE WT cells at 32 SPAC15E1.08,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC15E1.09,VIABLE WT cells at 25-32 SPAC15E1.10,VIABLE WT cells at 25-32 SPAC15F9.01c,VIABLE WT cells at 25-32 SPAC15F9.02,VIABLE WT cells at 25-32 SPAC15F9.03c,ESSENTIAL germinated spores long at 25-32 SPAC16.01,VIABLE WT cells at 25-32 SPAC16.02c,ESSENTIAL microcolonies long cells at 25-32 SPAC16.03c,VIABLE WT cells at 25-32 SPAC16.04,VIABLE WT cells at 25-32 SPAC16.05c,VIABLE WT cells at 25-32 SPAC1610.01,VIABLE small colonies long cells at 25-32 SPAC1610.02c,VIABLE small colonies WT cells- microcolonies skittle cells at 25-32 SPAC1610.03c,VIABLE WT cells at 25-32 SPAC1610.04,VIABLE WT cells at 25-32 SPAC1635.01,VIABLE WT cells at 25-32 SPAC1639.01c,VIABLE WT cells at 32 SPAC1639.02c,VIABLE WT cells at 25-32 SPAC167.01,VIABLE WT cells at 25-32 SPAC167.02,ESSENTIAL spores- germinated spores at 25-32 SPAC167.03c,ESSENTIAL spores- germinated spores at 25-32 SPAC167.04,VIABLE WT cells at 25-32 SPAC167.05,VIABLE WT cells at 25-32 SPAC167.06c,VIABLE WT cells at 25-32 SPAC167.07c,VIABLE WT cells at 25-32 SPAC1687.01,ESSENTIAL germinated spores long- often divide once at 25-32 SPAC1687.02,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC1687.03c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC1687.04,ESSENTIAL germinated spores long- some division at 25-32 SPAC1687.05,VIABLE WT cells at 25-32 SPAC1687.06c,VIABLE WT cells at 25-32 SPAC1687.07,VIABLE WT cells at 25-32 SPAC1687.08,VIABLE WT cells at 25-32 SPAC1687.09,VIABLE WT cells at 25-32 SPAC1687.10,VIABLE WT cells at 25-32 SPAC1687.11,ESSENTIAL spores- germinated spores at 25-32 SPAC1687.12c,VIABLE small colonies slightly misshapen vacuolated cells at 25-32 SPAC1687.13c,VIABLE WT cells at 25-32 SPAC1687.14c,VIABLE WT cells at 25-32 SPAC1687.15,VIABLE WT cells at 25-32 SPAC1687.16c,VIABLE WT cells at 25-32 SPAC1687.17c,VIABLE WT cells at 25-32 SPAC1687.18c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC1687.19c,VIABLE WT cells at 25-32 SPAC1687.20c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC1687.21,VIABLE WT cells at 25-32 SPAC1687.22c,VIABLE WT cells at 25-32 SPAC1687.23c,VIABLE WT cells at 32 SPAC16A10.01,VIABLE WT cells at 25-32 SPAC16A10.02,VIABLE WT cells at 25-32 SPAC16A10.03c,VIABLE WT cells at 32 SPAC16A10.04,VIABLE WT cells at 25-32 SPAC16A10.05c,VIABLE small colonies slightly misshapen cells at 32- some misshapen and dead cells SPAC16A10.06c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC16A10.07c,VIABLE WT cells at 25-32 SPAC16C9.01c,VIABLE WT cells at 25-32 SPAC16C9.02c,VIABLE WT cells at 25-32 SPAC16C9.03,ESSENTIAL spores- germinated spores at 25-32 SPAC16C9.04c,VIABLE WT cells at 25-32 SPAC16C9.05,VIABLE small colonies long curved cells at 25-32 SPAC16C9.06c,VIABLE WT cells at 25-32 SPAC16C9.07,VIABLE WT cells at 25-32 SPAC16E8.01,VIABLE slightly misshapen cells at 25-32 SPAC16E8.02,ESSENTIAL germinated spores slightly misshapen- divide once or twice at 25-32 SPAC16E8.03,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC16E8.04c,ESSENTIAL microcolonies slightly misshapen cells at 32- spores- germination at 25 SPAC16E8.05c,VIABLE WT cells at 25-32 SPAC16E8.06c,VIABLE misshapen cells at 25-32 SPAC16E8.07c,ESSENTIAL spores- germinated spores- some division slightly misshapen cells at 25-32 SPAC16E8.08,VIABLE WT cells at 32 SPAC16E8.09,VIABLE small colonies stubby cells at 25-32 SPAC16E8.10c,ESSENTIAL microcolonies skittle slightly misshapen cells at 25-32 SPAC16E8.11c,ESSENTIAL germinated spores branched slightly long- septated at 25-32 SPAC16E8.12c,VIABLE WT cells at 25-32 SPAC16E8.13,VIABLE WT cells at 25-32 SPAC16E8.14c,VIABLE WT cells at 25-32 SPAC16E8.15,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC16E8.16,ESSENTIAL spores- some germinated spores at 25-32 SPAC16E8.17c,VIABLE WT cells at 25-32 SPAC16E8.18,VIABLE WT cells at 32 SPAC1705.02,VIABLE WT cells at 25-32 SPAC1705.03c,VIABLE WT cells at 25-32 SPAC1751.01c,VIABLE WT cells at 25-32 SPAC1751.02c,ESSENTIAL microcolonies skittle slightly misshapen cells at 25-32 SPAC1751.03,ESSENTIAL germinated spores- microcolonies long cells at 25-32 SPAC1751.04,VIABLE WT cells at 25-32 SPAC1782.01,VIABLE WT cells at 25-32 SPAC1782.02c,VIABLE WT cells at 25-32 SPAC1782.03,ESSENTIAL spores- germinated spores at 25-32 SPAC1782.04,VIABLE WT cells at 25-32 SPAC1782.05,VIABLE small colonies rounded cells at 25-32 SPAC1782.06c,VIABLE WT cells at 25-32 SPAC1782.07,VIABLE WT cells at 25-32 SPAC1782.08c,VIABLE WT cells at 25-32 SPAC1782.09c,VIABLE slightly misshapen small cells at 25-32 SPAC1782.10c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC1782.11,VIABLE slightly misshapen vacuolated cells at 25- 32 SPAC1782.12c,VIABLE WT cells at 25-32 SPAC1783.01,VIABLE WT cells at 25-32 SPAC1783.02c,VIABLE WT cells at 32 cells slightly misshapen at 25-36 SPAC1783.03,ESSENTIAL microcolonies stubby cells at 32 cells more misshapen at 25 SPAC1783.04c,VIABLE small colonies long cells at 25-32 SPAC1783.05,VIABLE WT cells at 25-32 SPAC1783.06c,VIABLE WT cells at 25-32 SPAC1783.07c,VIABLE WT cells at 25-32 SPAC1783.08c,VIABLE WT cells at 25-32 SPAC1786.01c,VIABLE WT cells at 25-32 SPAC1786.02,VIABLE WT cells at 32 SPAC1786.03,ESSENTIAL spores- germinated spores at 25-32 SPAC1786.04,VIABLE WT cells at 25-32 SPAC17A2.01,VIABLE WT cells at 25-32 SPAC17A2.02c,VIABLE WT cells at 25-32 SPAC17A2.03c,ESSENTIAL germinated spores misshapen at 25-32 SPAC17A2.04c,ESSENTIAL microcolonies slightly misshapen curved cells at 25-32 SPAC17A2.05,VIABLE WT cells at 25-32 SPAC17A2.06c,VIABLE WT cells at 25-32 SPAC17A2.07c,VIABLE WT cells at 32 SPAC17A2.08c,ESSENTIAL germinated spores- some long at 25-32 SPAC17A2.09c,VIABLE WT cells at 25-32 SPAC17A2.10c,VIABLE WT cells at 25-32 SPAC17A2.11,VIABLE WT cells at 25-32 SPAC17A2.12,VIABLE WT cells at 25-32 SPAC17A2.13c,VIABLE WT cells at 25-32 SPAC17A2.14,VIABLE slightly misshapen cells at 25-32 SPAC17A5.01,VIABLE WT cells at 25-32 SPAC17A5.02c,VIABLE small colonies long cells- possibly diploidising at 25-32 SPAC17A5.03,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC17A5.04c,VIABLE WT cells at 25-32 SPAC17A5.05c,VIABLE WT cells at 25-32 SPAC17A5.06,ESSENTIAL spores at 25-32 SPAC17A5.07c,VIABLE slightly misshapen cells at 25- VIABLE/ESSENTIAL small colonies slightly misshapen cells at 32 SPAC17A5.08,VIABLE slightly stubby cells at 25-32 SPAC17A5.09c,VIABLE WT cells at 25-32 SPAC17A5.10,VIABLE WT cells at 25-32 SPAC17A5.11,VIABLE WT cells at 25-32 SPAC17A5.12,ESSENTIAL microcolonies rounded cells at 25-32 SPAC17A5.13,ESSENTIAL microcolonies rounded cells at 25-32 SPAC17A5.14,VIABLE long cells at 32 SPAC17A5.15c,ESSENTIAL spores at 25-32 SPAC17A5.16,VIABLE WT cells at 25-32 SPAC17A5.18c,VIABLE WT cells at 25-32 SPAC17C9.01c,ESSENTIAL germinated spores long- microcolonies with some long cells at 25-32 SPAC17C9.02c,VIABLE WT cells at 32 SPAC17C9.03,ESSENTIAL spores- germinated spores at 25-32 SPAC17C9.05c,VIABLE WT cells at 25-32 SPAC17C9.06,ESSENTIAL small colonies skittle cells at 25-32 SPAC17C9.07,VIABLE slightly misshapen cells at 25-32 SPAC17C9.08,VIABLE WT cells at 25-32 SPAC17C9.09c,VIABLE WT cells at 25-32 SPAC17C9.10,VIABLE WT cells at 25-32 SPAC17C9.11c,VIABLE WT cells at 25-32 SPAC17C9.12,VIABLE WT cells at 25-32 SPAC17C9.13c,VIABLE slightly misshapen cells at 25- ESSENTIAL germinated spores long- often divide at 32 SPAC17C9.14,VIABLE WT cells at 25-32 SPAC17C9.15c,VIABLE WT cells at 32 SPAC17C9.16c,VIABLE WT cells at 25-32 SPAC17D4.01,VIABLE WT cells at 32 SPAC17D4.02,ESSENTIAL germinated spores- often long at 25-32 SPAC17D4.03c,VIABLE WT cells at 25-32 SPAC17D4.04,VIABLE slightly misshapen- septated cells at 32 SPAC17G6.02c,VIABLE WT cells at 25-32 SPAC17G6.03,VIABLE WT cells at 25-32 SPAC17G6.04c,VIABLE small colonies rounded cells at 25-32 SPAC17G6.05c,VIABLE WT cells at 25-32 SPAC17G6.06,VIABLE WT cells at 25-32 SPAC17G6.07c,ESSENTIAL spores at 25-32 SPAC17G6.08,VIABLE WT cells at 25-32 SPAC17G6.09,ESSENTIAL spores- germinated spores slightly misshapen may divide once at 25-32 SPAC17G6.10,ESSENTIAL microcolonies misshapen- septated cells at 25-32 SPAC17G6.11c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC17G6.12,ESSENTIAL microcolonies long cells- possibly diploids at 25-32 SPAC17G6.13,VIABLE WT cells at 25-32 SPAC17G6.14c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC17G6.15c,VIABLE WT cells at 25-32 SPAC17G6.16c,ESSENTIAL spores- germinated spores misshapen at 25-32 SPAC17G6.17,VIABLE WT cells at 25-32 SPAC17G8.01c,ESSENTIAL germinated spores at 25-32 SPAC17G8.02,ESSENTIAL microcolonies rounded cells at 25-32 SPAC17G8.03c,ESSENTIAL microcolonies long cells at 25-32 SPAC17G8.04c,ESSENTIAL swollen spores at 25-32 SPAC17G8.05,VIABLE small colonies branched slightly long- septated cells at 25-32- cells almost dead at 36 SPAC17G8.06c,VIABLE small colonies WT cells at 25-32 SPAC17G8.07,VIABLE slightly curved cells at 25-32 SPAC17G8.08c,VIABLE WT cells at 25-32 SPAC17G8.09,VIABLE slightly long cells at 25-32 SPAC17G8.10c,VIABLE WT cells at 25-32 SPAC17G8.11c,VIABLE WT cells at 25-32 SPAC17G8.12,ESSENTIAL microcolonies misshapen cells- lysis at 25-32 SPAC17G8.13c,VIABLE slightly misshapen cells at 25-32 SPAC17G8.14c,VIABLE WT cells at 25-32 SPAC17H9.01,VIABLE WT cells at 25-32 SPAC17H9.02,ESSENTIAL microcolonies misshapen- septated cells at 25-32 SPAC17H9.03c,VIABLE WT cells at 25-32 SPAC17H9.04c,VIABLE WT cells at 25-32 SPAC17H9.05,ESSENTIAL spores- some germinated spores at 25-32 SPAC17H9.06c,VIABLE WT cells at 25-32 SPAC17H9.07,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC17H9.08,VIABLE small colonies slightly misshapen cells at 25-32 SPAC17H9.09c,VIABLE stubby cells at 25-32 SPAC17H9.10c,VIABLE small colonies long cells at 25-32 SPAC17H9.11,VIABLE WT cells at 25-32 SPAC17H9.12c,VIABLE WT cells at 25-32 SPAC17H9.13c,VIABLE WT cells at 25-32 SPAC17H9.14c,VIABLE WT cells at 25-32 SPAC17H9.16,ESSENTIAL microcolonies skittle cells at 25-32 SPAC17H9.17c,ESSENTIAL germinated spores- microcolonies rounded cells at 25-32 SPAC17H9.19c,VIABLE very small colonies long cells at 25- germinated spores long at 32 SPAC17H9.20,ESSENTIAL germinated spores- occasionally microcolonies slightly misshapen cells- some long cells at 25-32 SPAC1805.01c,VIABLE WT cells at 25-32 SPAC1805.02c,VIABLE WT cells at 25-32 SPAC1805.03c,VIABLE WT cells at 25-32 SPAC1805.04,VIABLE WT cells at 25-32 SPAC1805.05,VIABLE WT cells at 25-32 SPAC1805.06c,VIABLE WT cells at 25-32 SPAC1805.07c,VIABLE slightly misshapen cells at 25-32 SPAC1805.08,VIABLE WT cells at 25-32 SPAC1805.09c,VIABLE WT cells at 25-32 SPAC1805.10,VIABLE WT cells at 25-32 SPAC1805.11c,VIABLE WT cells at 25-32 SPAC1805.12c,VIABLE WT cells at 25-32 SPAC1805.13,VIABLE WT cells at 25-32 SPAC1805.14,VIABLE WT cells at 32 cells slightly misshapen at 25 SPAC1805.15c,VIABLE WT cells at 25-32 SPAC1805.16c,VIABLE WT cells at 25-32 SPAC1805.17,ESSENTIAL germinated spores long- occasionally divide at 25-32 SPAC1834.01,ESSENTIAL spores at 25-32 SPAC1834.02,ESSENTIAL spores- some germinated spores at 25-32 SPAC1834.03c,VIABLE WT cells at 25-32 SPAC1834.04,VIABLE WT cells at 25-32 SPAC1834.05,VIABLE WT cells at 25-32- some dead cells SPAC1834.06c,ESSENTIAL microcolonies rounded cells- lysis at 25-32 SPAC1834.07,VIABLE slightly misshapen cells at 25-32 SPAC1834.08,VIABLE WT cells at 25-32 SPAC1834.09,VIABLE WT cells at 25-32 SPAC1834.10c,VIABLE WT cells at 32 SPAC1834.11c,ESSENTIAL germinated spores at 25-32 SPAC1851.02,VIABLE WT cells at 25-32 SPAC1851.03,VIABLE very small colonies rounded cells at 25-32- lysis after 48h SPAC1851.04c,VIABLE small colonies slightly misshapen cells at 32 SPAC186.01,VIABLE WT cells at 25-32 SPAC186.02c,VIABLE WT cells at 25-32 SPAC186.03,VIABLE WT cells at 25-32 SPAC186.05c,VIABLE WT cells at 25-32 SPAC186.06,VIABLE WT cells at 25-32, SPAC186.07c,VIABLE WT cells at 25-32, SPAC186.08c,VIABLE WT cells at 25-32, SPAC186.09,VIABLE WT cells at 25-32, SPAC18B11.02c,VIABLE WT cells at 25-32, SPAC18B11.03c,VIABLE WT cells at 25-32, SPAC18B11.04,VIABLE WT cells at 25-32, SPAC18B11.05,ESSENTIAL germinated spores at 32- microcolonies rounded cells at 25,x SPAC18B11.06,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32, SPAC18B11.07c,VIABLE small colonies long cells- often curved at 25-32, SPAC18B11.08c,VIABLE WT cells at 25-32, SPAC18B11.09c,VIABLE WT cells at 25-32, SPAC18B11.10,VIABLE WT cells at 25-32, SPAC18B11.11,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32, SPAC18G6.01c,VIABLE WT cells at 25-32, SPAC18G6.02c,VIABLE WT cells at 25-32, SPAC18G6.03,ESSENTIAL spores at 25-32 SPAC18G6.04c,VIABLE WT cells at 25-32 SPAC18G6.05c,VIABLE WT cells at 25-32 SPAC18G6.06,ESSENTIAL spores at 25-32 SPAC18G6.07c,ESSENTIAL germinated spores long at 25- germinated spores slightly long at 32 SPAC18G6.09c,VIABLE small colonies long cells at 25-32 SPAC18G6.10,VIABLE WT cells at 25-32 SPAC18G6.11c,ESSENTIAL spores- germinated spores at 25-32 SPAC18G6.12c,VIABLE WT cells at 25-32 SPAC18G6.13,VIABLE small colonies slightly misshapen cells at 25-32 SPAC18G6.14c,ESSENTIAL spores at 25-32 SPAC18G6.15,VIABLE slightly curved cells at 25-32- many misshapen cells on exit from stationary phase SPAC1952.01,ESSENTIAL germinated spores slightly misshapen- often divide at 25-32 SPAC1952.02,VIABLE WT cells at 25-32 SPAC1952.03,VIABLE WT cells at 25-32 SPAC1952.05,VIABLE WT cells at 25-32 SPAC1952.06c,VIABLE WT cells at 25-32 SPAC1952.07,VIABLE WT cells at 25-32 SPAC1952.08c,VIABLE WT cells at 25-32 SPAC1952.09c,VIABLE WT cells at 25-32 SPAC1952.10c,VIABLE WT cells at 25-32 SPAC1952.11c,VIABLE WT cells at 25-32 SPAC1952.12c,VIABLE WT cells at 25-32 SPAC1952.13,ESSENTIAL germinated spores long at 25-32- often divide at 32 SPAC1952.14c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC1952.15c,VIABLE WT cells at 25-32 SPAC1952.16,VIABLE WT cells at 25-32 SPAC1952.17c,VIABLE WT cells at 25-32 SPAC19A8.01c,VIABLE WT cells at 25-32 SPAC19A8.02,VIABLE WT cells at 25-32 SPAC19A8.03,VIABLE WT cells at 25-32 SPAC19A8.04,VIABLE WT cells at 25-32 SPAC19A8.05c,VIABLE WT cells at 25-32 SPAC19A8.06,ESSENTIAL spores at 25-32 SPAC19A8.07c,ESSENTIAL spores- germinated spores- often long at 25-32 SPAC19A8.08,VIABLE WT cells at 25-32 SPAC19A8.09,ESSENTIAL spores- some germinated spores at 25-32 SPAC19A8.10,VIABLE WT cells at 25-32 SPAC19A8.11c,VIABLE small colonies WT cells at 25-32 SPAC19A8.12,ESSENTIAL germinated spores long- microcolonies long cells at 25-32 SPAC19A8.13,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC19A8.14,VIABLE WT cells at 25-32 SPAC19A8.15,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPAC19B12.01,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPAC19B12.02c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPAC19B12.03,ESSENTIAL microcolonies rounded cells at 25-32 SPAC19B12.04,VIABLE WT cells at 25-32 SPAC19B12.05c,ESSENTIAL microcolonies misshapen curved cells at 25-32 SPAC19B12.06c,VIABLE WT cells at 25-32 SPAC19B12.07c,VIABLE WT cells at 25-32 SPAC19B12.08,VIABLE WT cells at 25-32 SPAC19B12.10,VIABLE WT cells at 25-32 SPAC19B12.11c,VIABLE WT cells at 25-32 SPAC19B12.12c,VIABLE WT cells at 25-32 SPAC19D5.01,VIABLE WT cells at 25-32 SPAC19D5.02c,VIABLE WT cells at 25-32 SPAC19D5.03,VIABLE WT cells at 25-32 SPAC19D5.04,ESSENTIAL germinated spores long at 25-32 SPAC19D5.05c,ESSENTIAL spores- germinated spores at 25-32 SPAC19D5.06c,VIABLE WT cells at 25-32 SPAC19D5.07,VIABLE WT cells at 25-32 SPAC19D5.11c,VIABLE WT cells at 25-32 SPAC19E9.01c,VIABLE WT cells at 25-32 SPAC19E9.02,VIABLE small colonies slightly misshapen cells at 25-32 SPAC19E9.03,VIABLE WT cells at 25-32- dead cells causes wavy edges to colony SPAC19G12.01c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC19G12.02c,VIABLE WT cells at 25-32 SPAC19G12.03,VIABLE WT cells at 25-32 SPAC19G12.04,VIABLE WT cells at 25-32 SPAC19G12.05,VIABLE WT cells at 25-32 SPAC19G12.06c,VIABLE WT cells at 25-32 SPAC19G12.07c,ESSENTIAL microcolonies long cells- often curved at 25-32 SPAC19G12.08,VIABLE WT cells at 25-32 SPAC19G12.09,VIABLE WT cells at 25-32 SPAC19G12.10c,VIABLE WT cells at 25-32 SPAC19G12.11,VIABLE WT cells at 25-32 SPAC19G12.12,VIABLE small colonies slightly misshapen cells at 25-32 SPAC19G12.13c,VIABLE WT cells at 25-32 SPAC19G12.14,ESSENTIAL spores at 25-32 SPAC19G12.15c,VIABLE WT cells at 25-32 SPAC19G12.16c,VIABLE WT cells at 25-32 SPAC1A6.01c,VIABLE WT cells at 25-32 SPAC1A6.02,ESSENTIAL small colonies slightly misshapen cells at 25-32 SPAC1A6.03c,VIABLE WT cells at 25-32 SPAC1A6.04c,VIABLE WT cells at 25-32 SPAC1A6.05c,VIABLE WT cells at 25-32 SPAC1A6.06c,VIABLE WT cells at 25-32 SPAC1A6.07,VIABLE WT cells at 25-32 SPAC1A6.08c,VIABLE WT cells at 25-32 SPAC1A6.09c,VIABLE WT cells at 25-32 SPAC1A6.10,VIABLE WT cells at 25-32 SPAC1B1.01,ESSENTIAL spores- germinated spores slightly misshapen divide once or twice at 25-32 SPAC1B1.02c,VIABLE WT cells at 25-32 SPAC1B1.03c,ESSENTIAL spores at 25-32 SPAC1B1.04c,VIABLE slightly misshapen cells at 25-32 SPAC1B2.02c,VIABLE small colonies slightly misshapen cells- some microcolonies skittle cells at 25-32. SPAC1B2.03c,VIABLE WT cells at 25-32 SPAC1B2.04,VIABLE WT cells at 25-32 SPAC1B2.05,ESSENTIAL germinated spores long- microcolonies with some long cells at 25-32 SPAC1B3.01c,VIABLE WT cells at 25-32 SPAC1B3.02c,VIABLE WT cells at 25-32 SPAC1B3.03c,VIABLE WT cells at 25-32 SPAC1B3.04c,VIABLE WT cells at 25-32 SPAC1B3.05,VIABLE WT cells at 25-32 SPAC1B3.06c,VIABLE WT cells at 25-32 SPAC1B3.07c,VIABLE WT cells at 25-32 SPAC1B3.08,VIABLE WT cells at 25-32 SPAC1B3.09c,ESSENTIAL spores- germinated spores- microcolonies slightly misshapen cells at 32- spores- germinated spores at 25 SPAC1B3.10c,VIABLE WT cells at 25-32 SPAC1B3.11c,VIABLE WT cells at 25-32 SPAC1B3.12c,ESSENTIAL germinated spores slightly long at 25-32 SPAC1B3.13,ESSENTIAL germinated spores- often long- some division at 25-32 SPAC1B3.14,ESSENTIAL spores- germinated spores at 25-32 SPAC1B3.15c,VIABLE WT cells at 25-32 SPAC1B3.16c,VIABLE small colonies WT with some long cells at 25-32 SPAC1B3.17,VIABLE WT cells at 25-32 SPAC1B3.18c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC1B3.20,VIABLE WT cells at 25-32 SPAC1B9.02c,VIABLE WT cells at 25-32 SPAC1B9.03c,ESSENTIAL germinated spores at 25-32 SPAC1D4.01,VIABLE WT cells at 25-32 SPAC1D4.02c,VIABLE WT cells at 25-32 SPAC1D4.03c,VIABLE WT cells at 25-32 SPAC1D4.04,ESSENTIAL spores at 25-32 SPAC1D4.05c,VIABLE WT cells at 25-32 SPAC1D4.06c,VIABLE WT cells at 25-32 SPAC1D4.08,ESSENTIAL germinated spores at 25-32 SPAC1D4.09c,VIABLE WT cells at 25-32 SPAC1D4.10,ESSENTIAL spores- germinated spores- occasionally microcolonies misshapen cells at 25-32 SPAC1D4.11c,"VIABLE WT cells at 25-32"" piled up"" colony morphology" SPAC1D4.12,ESSENTIAL germinated spores long- septated- some division and swollen cells at 25-32 SPAC1D4.13,VIABLE WT cells at 25-32 SPAC1D4.14,ESSENTIAL microcolonies long cells at 25-32 SPAC1F12.02c,VIABLE WT cells at 25-32 SPAC1F12.04c,VIABLE WT cells at 25-32 SPAC1F12.05,VIABLE WT cells at 25-32 SPAC1F12.06c,VIABLE WT cells at 25-32 SPAC1F12.07,VIABLE small colonies WT cells at 25-32 SPAC1F12.08,ESSENTIAL microcolonies small cells at 25-32 SPAC1F12.09,ESSENTIAL spores- germinated spores misshapen lysis at 25-32 SPAC1F12.10c,VIABLE WT cells at 25-32 SPAC1F3.01,SUPPRESSED SPAC1F3.03,VIABLE WT cells at 25-32 SPAC1F3.05,VIABLE WT cells at 25-32 SPAC1F3.06c,VIABLE WT cells at 25-32 SPAC1F3.07c,VIABLE slightly misshapen cells at 25-32 SPAC1F3.09,VIABLE WT cells at 25-32 SPAC1F3.10c,VIABLE slightly skittle cells at 32- cells slightly misshapen at 25 SPAC1F5.02,ESSENTIAL microcolonies rounded cells at 25-32 SPAC1F5.03c,VIABLE WT cells at 25-32 SPAC1F5.04c,ESSENTIAL germinated spores long- slight swollen at 25-32 SPAC1F5.05c,VIABLE WT cells at 25- stubby- septated cells at 32- cells dead at 36 SPAC1F5.06,ESSENTIAL spores- germinated spores at 25-32 some division SPAC1F5.07c,VIABLE WT cells at 25-32 SPAC1F5.08c,VIABLE WT cells at 25-32 SPAC1F5.09c,VIABLE WT cells at 25-32 SPAC1F5.10,VIABLE small colonies slightly long cells at 25-32 SPAC1F5.11c,ESSENTIAL microcolonies long branched- septated cells at 25-32 SPAC1F7.01c,VIABLE slightly curved cells at 32 SPAC1F7.02c,ESSENTIAL spores at 25-32 SPAC1F7.03,ESSENTIAL spores at 25-32 SPAC1F7.04,VIABLE WT cells at 25-32 SPAC1F7.05,ESSENTIAL germinated spores long at 25-32 SPAC1F7.06,VIABLE WT cells at 25-32 SPAC1F7.07c,VIABLE WT cells at 25-32 SPAC1F7.08,VIABLE WT cells at 25-32 SPAC1F7.09c,VIABLE WT cells at 25-32 SPAC1F7.10,VIABLE WT cells at 25-32 SPAC1F7.11c,VIABLE WT cells at 25-32 SPAC1F7.12,VIABLE WT cells at 25-32 SPAC1F7.13c,VIABLE very small colonies WT cells at 25-32 SPAC1F8.01,VIABLE WT cells at 25-32 SPAC1F8.02c,VIABLE WT cells at 25-32 SPAC1F8.03c,VIABLE WT cells at 25-32- poor growth at 36 SPAC1F8.04c,VIABLE WT cells at 25-32 SPAC1F8.05,VIABLE WT cells at 25-32 SPAC1F8.06,VIABLE WT cells at 25-32 SPAC1F8.07c,ESSENTIAL microcolonies long cells at 25-32 SPAC1F8.08,VIABLE WT cells at 25-32 SPAC20G4.01,VIABLE WT cells at 25-32 SPAC20G4.02c,VIABLE WT cells at 25-32 SPAC20G4.03c,VIABLE WT cells at 25-32 SPAC20G4.04c,VIABLE WT cells at 25-32 SPAC20G4.05c,VIABLE WT cells at 25-32 SPAC20G4.06c,ESSENTIAL microcolonies long cells at 25-32 SPAC20G4.07c,VIABLE WT cells at 25-32 SPAC20G4.08,VIABLE WT cells at 25-32 SPAC20G8.01,ESSENTIAL germinated spores long at 25-32 SPAC20G8.02,VIABLE WT cells at 25-32 SPAC20G8.03,ESSENTIAL spores at 25-32 SPAC20G8.04c,VIABLE WT cells at 25-32 SPAC20G8.05c,ESSENTIAL germinated spores long at 25-32 SPAC20G8.06,ESSENTIAL small colonies rounded cells at 32- cells longer at 25 SPAC20G8.07c,VIABLE WT cells at 25-32 SPAC20G8.08c,VIABLE WT cells at 25-32 SPAC20G8.09c,VIABLE WT cells at 25-32 SPAC20G8.10c,VIABLE WT cells at 25-32 SPAC20H4.01,ESSENTIAL spores- germinated spores long at 25-32 SPAC20H4.02,VIABLE small colonies slightly misshapen cells at 25-32 SPAC20H4.03c,VIABLE WT cells at 25-32 SPAC20H4.04,VIABLE WT cells at 25-32 SPAC20H4.05c,VIABLE WT cells at 25-32 SPAC20H4.06c,VIABLE WT cells at 32 SPAC20H4.07,VIABLE long cells at 25-32 SPAC20H4.08,VIABLE WT cells at 25-32 SPAC20H4.09,VIABLE WT cells at 25-32 SPAC20H4.10,VIABLE WT cells at 25-32 SPAC20H4.11c,VIABLE slightly misshapen cells at 25-32 SPAC212.01c,VIABLE WT cells at 32 SPAC212.02,VIABLE WT cells at 25-32 SPAC212.03,VIABLE WT cells at 32 SPAC212.04c,VIABLE WT cells at 25-32 SPAC212.08c,VIABLE WT cells at 32 SPAC212.11,VIABLE WT cells at 25-32 SPAC21E11.03c,VIABLE WT cells at 25-32 SPAC21E11.04,VIABLE slightly misshapen cells at 25-32 SPAC21E11.05c,VIABLE WT cells at 25-32 SPAC21E11.06,ESSENTIAL spores at 25-32 SPAC21E11.07,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC21E11.08,ESSENTIAL microcolonies slightly misshapen cells- some long cells- some lysis at 25-32 SPAC222.03c,ESSENTIAL microcolonies slightly skittle cells at 25-32 SPAC222.04c,VIABLE small colonies misshapen cells at 25-32- occasionally very long possibly diploidises SPAC222.05c,VIABLE WT cells at 25-32- possibly poor germination SPAC222.06,ESSENTIAL spores at 25-32 SPAC222.07c,VIABLE WT cells at 25-32 SPAC222.08c,VIABLE WT cells at 25-32 SPAC222.09,ESSENTIAL spores at 25-32 SPAC222.10c,ESSENTIAL germinated spores swollen branched slightly long at 25-32 SPAC222.11,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC222.12c,VIABLE WT cells at 25-32 SPAC222.13c,VIABLE WT cells at 25-32 SPAC222.14c,VIABLE WT cells at 25-32 SPAC222.15,VIABLE WT cells at 25-32 SPAC222.16c,VIABLE WT cells at 25-32 SPAC227.01c,VIABLE stubby cells at 32 cells barely growing at 25 SPAC227.02c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC227.03c,VIABLE WT cells at 25-32 SPAC227.04,VIABLE WT cells at 25-32 SPAC227.05,VIABLE WT cells at 25-32 SPAC227.06,VIABLE WT cells at 25-32 SPAC227.07c,VIABLE small colonies rounded and WT cells at 25-32 SPAC227.08c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC227.09,ESSENTIAL microcolonies skittle- rounded cells at 25-32 SPAC227.10,VIABLE slightly misshapen cells at 25-32- cells more misshapen- occasionally slightly curved at 36 SPAC227.11c,VIABLE WT cells at 25-32 SPAC227.12,ESSENTIAL germinated spores long at 25-32 SPAC227.13c,VIABLE WT cells at 25-32 SPAC227.14,VIABLE WT cells at 25-32 SPAC227.15,VIABLE WT cells at 25-32 SPAC227.16c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC227.17c,VIABLE WT cells at 25-32 SPAC227.18,VIABLE WT cells at 25-32 SPAC22A12.01c,VIABLE WT cells- some germination long at 25-32- SPAC22A12.02c,VIABLE WT cells at 25-32 SPAC22A12.03c,VIABLE WT cells at 25-32 SPAC22A12.04c,VIABLE small colonies WT cells at 25-32 SPAC22A12.05,ESSENTIAL germinated spores slightly misshapen- occasionally divide once or twice- at 25-32 SPAC22A12.06c,VIABLE WT cells at 25-32 SPAC22A12.07c,VIABLE small colonies misshapen septated at 32- cells less misshapen at 25 SPAC22A12.08c,ESSENTIAL small colonies skittle cells at 25-32 SPAC22A12.09c,ESSENTIAL germinated spores misshapen- occasionally microcolonies septated cells at 25-32 SPAC22A12.10, VIABLE WT cells at 25-32 SPAC22A12.11,VIABLE WT cells at 25-32 SPAC22A12.12c,ESSENTIAL germinated spores- occasionally long at 25-32 SPAC22A12.13,ESSENTIAL germinated spores slightly misshapen- often divide once at 25-32 SPAC22A12.14c,VIABLE WT cells at 25-32 SPAC22A12.15c,ESSENTIAL spores at 25-32 SPAC22A12.16,VIABLE WT cells at 25-32 SPAC22A12.17c,VIABLE WT cells at 25-32 SPAC22E12.01,VIABLE WT cells at 25-32 SPAC22E12.02,ESSENTIAL microcolonies long cells- colonies appear dark at 25-32 SPAC22E12.03c,VIABLE WT cells at 25-32 SPAC22E12.04,VIABLE WT cells at 25-32 SPAC22E12.05c,VIABLE WT cells at 25-32 SPAC22E12.06c,VIABLE WT cells at 25-32 SPAC22E12.07,ESSENTIAL spores at 25-32 SPAC22E12.08,ESSENTIAL microcolonies long cells at 25- cells more misshapen at 32 SPAC22E12.09c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC22E12.10c,ESSENTIAL spores- germinated spores- occasional microcolonies of WT/ rounded cells at 25-32 SPAC22E12.11c,VIABLE WT cells at 32 cells slightly misshapen at 25 SPAC22E12.13c,ESSENTIAL spores- germinated spores at 25-32 SPAC22E12.14c,VIABLE WT cells at 25-32 SPAC22E12.16c,ESSENTIAL spores- germinated spores at 25-32 SPAC22E12.17c,ESSENTIAL germinated spores at 25-32 SPAC22E12.18,VIABLE very small colonies WT cells at 25-32 SPAC22E12.19,VIABLE WT cells at 25-32 SPAC22F3.02,VIABLE WT cells at 25-32 SPAC22F3.03c,VIABLE WT cells with some long cells at 25-32- long cells at 36 SPAC22F3.04,VIABLE WT cells at 25-32 SPAC22F3.05c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC22F3.06c,VIABLE WT cells at 25-32 SPAC22F3.07c,VIABLE WT cells at 25-32 SPAC22F3.08c,VIABLE WT cells at 25-32 SPAC22F3.09c,VIABLE small colonies WT cells at 25-32 SPAC22F3.10c,VIABLE small colonies WT cells at 25-32 SPAC22F3.11c,VIABLE WT cells at 25-32 SPAC22F3.12c,VIABLE WT cells at 25-32 SPAC22F3.13,VIABLE small colonies WT cells at 25-32 SPAC22F8.02c,VIABLE WT cells at 25-32 SPAC22F8.03c,VIABLE WT cells at 25-32 SPAC22F8.04,VIABLE WT cells at 25-32 SPAC22F8.05,VIABLE WT cells at 25-32 SPAC22F8.06,ESSENTIAL spores- germinated spores at 25-32 SPAC22F8.07c,VIABLE WT cells at 25-32 SPAC22F8.08,ESSENTIAL spores- some germinated spores at 25-32 SPAC22F8.09,VIABLE WT cells at 25-32 SPAC22F8.10c,ESSENTIAL spores- germinated spores at 25-32 SPAC22F8.11,VIABLE small colonies misshapen cells at 32- cells slightly misshapen at 25 SPAC22F8.12c,VIABLE long cells at 25-32 SPAC22G7.01c,VIABLE WT cells at 25-32 SPAC22G7.02,VIABLE slightly misshapen cells at 25-32 SPAC22G7.03,VIABLE WT cells at 25-32 SPAC22G7.04,VIABLE WT cells at 25-32 SPAC22G7.05,VIABLE WT cells at 25-32 SPAC22G7.06c,VIABLE WT cells at 25-32 SPAC22G7.07c,VIABLE WT cells at 25-32 SPAC22G7.08,VIABLE WT cells at 25-32 SPAC22G7.09c,ESSENTIAL microcolonies long curved cells at 25-32 SPAC22G7.10,ESSENTIAL spores- germinated spores at 25-32 SPAC22G7.11c,VIABLE WT cells at 32 SPAC22H10.02,VIABLE WT cells at 25-32 SPAC22H10.03c,VIABLE WT cells at 25-32 SPAC22H10.04,VIABLE WT cells at 25-32 SPAC22H10.05c,ESSENTIAL spores- germinated spores at 25-32 SPAC22H10.07,VIABLE small colonies rounded cells at 25-32 SPAC22H10.08,VIABLE WT cells at 25-32 SPAC22H10.09,VIABLE WT cells at 25-32 SPAC22H10.10,ESSENTIAL germinated spores misshapen- some division at 25-32 SPAC22H10.11c,VIABLE small colonies WT cells- occasionally curved at 25-32 SPAC22H10.12c,ESSENTIAL germinated spores at 25-32 SPAC22H10.13,VIABLE WT cells at 25-32 SPAC22H12.01c,VIABLE WT cells at 25-32 SPAC22H12.02,VIABLE WT cells at 25-32 SPAC22H12.03,VIABLE WT cells at 25-32 SPAC22H12.04c,VIABLE WT cells at 25-32 SPAC22H12.05c,VIABLE WT cells at 25-32 SPAC23A1.02c,VIABLE WT cells at 25-32 SPAC23A1.03,VIABLE WT cells at 25-32 SPAC23A1.04c,VIABLE WT cells at 25-32 SPAC23A1.05,ESSENTIAL germinated spores at 25-32 SPAC23A1.06c,VIABLE WT cells at 25-32 SPAC23A1.07,VIABLE WT cells at 25-32 SPAC23A1.08c,ESSENTIAL spores- germinated spores at 25-32 SPAC23A1.09,VIABLE WT cells at 25-32 SPAC23A1.11,VIABLE WT cells at 25-32 SPAC23A1.12c,ESSENTIAL spores at 25-32 SPAC23A1.14c,VIABLE WT cells at 25-32 SPAC23A1.15c,VIABLE WT cells at 25-32 SPAC23A1.16c,ESSENTIAL germinated spores long misshapen- septated at 32- microcolonies long cells at 25 SPAC23A1.17,VIABLE WT cells at 25-32 SPAC23A1.18c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC23A1.19c,ESSENTIAL spores- germinated spores at 32- some colonies WT cells at 25 SPAC23C11.01,VIABLE WT cells at 25-32 SPAC23C11.02c,VIABLE small colonies WT cells at 25-32 SPAC23C11.03,ESSENTIAL spores- some germinated spores at 25-32 SPAC23C11.04c,VIABLE small colonies slightly long cells at 25-32 SPAC23C11.05,ESSENTIAL spores- germinated spores at 25-32 SPAC23C11.06c,VIABLE WT cells at 25-32 SPAC23C11.07,VIABLE WT cells at 25-32 SPAC23C11.08,VIABLE WT cells at 32 SPAC23C11.09,ESSENTIAL spores at 25-32 SPAC23C11.10,VIABLE small colonies long slightly misshapen cells at 25-32 SPAC23C11.11,ESSENTIAL small colonies rounded cells at 25-32- lysis after 48h SPAC23C11.12,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPAC23C11.13c,VIABLE small colonies long cells at 25-32 SPAC23C11.14,VIABLE WT cells at 25-32 SPAC23C11.15,VIABLE WT cells at 25-32 SPAC23C11.16,ESSENTIAL germinated spores long at 25-32 SPAC23C11.17,ESSENTIAL germinated spores- occasionally divide at 25-32 SPAC23C4.02,VIABLE WT cells at 25-32 SPAC23C4.03,VIABLE WT cells at 25-32 SPAC23C4.05c,VIABLE WT cells at 25-32 SPAC23C4.06c,VIABLE WT cells at 25-32 SPAC23C4.07,VIABLE WT cells at 25-32 SPAC23C4.08,VIABLE WT cells at 25-32 SPAC23C4.09c,VIABLE WT cells at 25-32 SPAC23C4.10,ESSENTIAL spores- germinated spores at 32- microcolonies misshapen cells at 25 SPAC23C4.11,VIABLE WT cells at 25-32 SPAC23C4.12,VIABLE WT cells at 25-32 SPAC23C4.13,ESSENTIAL spores- germinated spores at 25-32 SPAC23C4.14,ESSENTIAL germinated spores at 25-32 SPAC23C4.15,ESSENTIAL spores- germinated spores at 25-32 SPAC23C4.16c,VIABLE WT cells at 25-32 SPAC23C4.17,VIABLE WT cells at 25-32 SPAC23C4.18c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPAC23C4.19,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC23D3.01,VIABLE WT cells at 25-32 SPAC23D3.02,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC23D3.03c,VIABLE WT cells at 25-32 SPAC23D3.04c,VIABLE WT cells at 25-32 SPAC23D3.06c,ESSENTIAL germinated spores long at 25-32 SPAC23D3.07,ESSENTIAL spores- germinated spores at 25-32 SPAC23D3.08,ESSENTIAL microcolonies long- wide cells at 25-32 SPAC23D3.09,VIABLE slightly misshapen cells at 25-32 SPAC23D3.10c,VIABLE WT cells at 25-32 SPAC23D3.11,VIABLE WT cells at 25-32 SPAC23D3.12,VIABLE WT cells at 25-32 SPAC23D3.13c,VIABLE WT cells at 25-32 SPAC23E2.01,VIABLE WT cells at 25-32 SPAC23E2.02,ESSENTIAL microcolonies curved septated cells at 25-32 SPAC23E2.03c,VIABLE WT cells at 25-32 SPAC23G3.01,ESSENTIAL spores- germinated spores at 25-32 SPAC23G3.02c,VIABLE WT cells at 25-32 SPAC23G3.03,VIABLE WT cells at 25-32 SPAC23G3.04,VIABLE WT cells at 25-32 SPAC23G3.05c,VIABLE WT cells at 25-32 SPAC23G3.06,ESSENTIAL spores at 25-32 SPAC23G3.07c,VIABLE WT cells at 25-32 SPAC23G3.08c,VIABLE WT cells at 25-32 SPAC23G3.09,ESSENTIAL germinated spores slightly misshapen- occasionally divide once or twice- at 25-32 SPAC23G3.10c,VIABLE WT cells at 25-32 SPAC23G3.11,ESSENTIAL germinated spores at 25- germinated spores- occasionally long at 32 SPAC23G3.12c,VIABLE WT cells at 25-32 SPAC23H3.02c,ESSENTIAL germinated spores long- some division at 25-32 SPAC23H3.03c,VIABLE slightly long cells at 25-32 SPAC23H3.04,VIABLE slightly misshapen cells at 25-32 SPAC23H3.05c,VIABLE WT cells at 25-32 SPAC23H3.06,VIABLE WT cells at 25-32 SPAC23H3.07c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC23H3.08c,VIABLE WT cells at 25-32 SPAC23H3.09c,VIABLE WT cells at 25-32 SPAC23H3.10,ESSENTIAL spores- germinated spores long wide at 25-32 SPAC23H3.11c,VIABLE WT cells at 25-32 SPAC23H3.12c,VIABLE WT cells at 25-32 SPAC23H3.13c,VIABLE small colonies slightly misshapen small cells at 25-32 SPAC23H3.14,VIABLE WT cells at 25-32 SPAC23H3.15c,VIABLE slightly curved slightly misshapen cells at 25-32- cells dead at 36 SPAC23H4.01c,VIABLE WT cells at 25-32 SPAC23H4.02,VIABLE WT cells at 25-32 SPAC23H4.03c,ESSENTIAL spores- germinated spores at 25-32 SPAC23H4.04,ESSENTIAL microcolonies skittle cells at 25-32 SPAC23H4.06,ESSENTIAL spores- germinated spores at 25-32 SPAC23H4.07c,ESSENTIAL spores- germinated spores at 25-32 SPAC23H4.08,VIABLE WT cells at 25-32 SPAC23H4.09,VIABLE WT cells at 25-32 SPAC23H4.10c,VIABLE WT cells at 25-32 SPAC23H4.11c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC23H4.12,VIABLE long curved cells at 25-32- cells longer and misshapen at 36 SPAC23H4.14,VIABLE very small colonies slightly misshapen cells at 25-32 SPAC23H4.15,ESSENTIAL spores- germinated spores- often long at 25 some division at 32 SPAC23H4.16c,VIABLE WT cells at 25-32 SPAC23H4.17c,VIABLE WT cells at 25-32 SPAC23H4.18c,ESSENTIAL small colonies long cells at 25-32 SPAC24B11.05,VIABLE WT cells at 25-32 SPAC24B11.06c,VIABLE long cells at 25-32 SPAC24B11.07c,VIABLE WT cells at 25-32 SPAC24B11.08c,VIABLE WT cells at 25-32 SPAC24B11.09,VIABLE slightly misshapen septated cells at 25-32 SPAC24B11.10c,VIABLE slightly misshapen cells at 25-32 SPAC24B11.11c,ESSENTIAL germinated spores long- lysis at 25-32 SPAC24B11.12c,VIABLE WT cells at 25-32 SPAC24B11.13,VIABLE slightly misshapen cells at 32 SPAC24C9.02c,VIABLE WT cells at 25-32 SPAC24C9.03,ESSENTIAL spores- germinated spores at 25-32 SPAC24C9.04,ESSENTIAL microcolonies curved branched septated slightly long cells at 25-32 SPAC24C9.05c,VIABLE WT cells at 25-32 SPAC24C9.06c,ESSENTIAL microcolonies long cells at 25-32 SPAC24C9.07c,VIABLE WT cells at 25-32 SPAC24C9.08,VIABLE WT cells at 25-32 SPAC24C9.09,ESSENTIAL small colonies skittle cells at 25-32 SPAC24C9.10c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC24C9.11,ESSENTIAL germinated spores- often long at 25-32 SPAC24C9.12c,VIABLE WT cells at 25-32 SPAC24C9.13c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC24C9.14,VIABLE WT cells at 25-32 SPAC24C9.15c,VIABLE WT cells at 25-32 SPAC24C9.16c,VIABLE WT cells at 25-32 SPAC24H6.01c,ESSENTIAL germinated spores misshapen- microcolonies misshapen cells at 25-32 SPAC24H6.02c,VIABLE WT cells at 25-32 SPAC24H6.03,VIABLE WT cells with some long cells at 25-32 SPAC24H6.04,VIABLE WT cells at 25-32 SPAC24H6.05,ESSENTIAL germinated spores long at 25-32 SPAC24H6.06,ESSENTIAL germinated spores at 25-32 SPAC24H6.07, VIABLE WT cells at 25-32 SPAC24H6.08,VIABLE WT cells at 25-32 SPAC24H6.09,VIABLE slightly misshapen cells at 25-32 SPAC24H6.10c,VIABLE WT cells at 25-32 SPAC24H6.11c,VIABLE WT cells at 25-32 SPAC24H6.12c,ESSENTIAL small colonies long cells at 25-32 SPAC24H6.13,VIABLE WT cells at 25-32 SPAC25A8.01c,VIABLE small colonies WT cells at 25-32 SPAC25A8.02,VIABLE WT cells at 25-32 SPAC25A8.03c,VIABLE WT cells at 25-32 SPAC25B8.01,VIABLE WT cells at 25-32 SPAC25B8.02,ESSENTIAL small colonies long cells at 25-32 SPAC25B8.03,VIABLE WT cells at 32 cells slightly misshapen at 25 SPAC25B8.04c,VIABLE WT cells at 25-32 SPAC25B8.05,VIABLE WT cells at 25-32 SPAC25B8.06c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC25B8.07c,VIABLE WT cells at 25-32 SPAC25B8.08,VIABLE WT cells at 25-32 SPAC25B8.09,VIABLE WT cells at 25-32 SPAC25B8.10,VIABLE WT cells at 25-32 SPAC25B8.11,VIABLE WT cells at 25-32 SPAC25B8.13c,VIABLE WT cells at 25-32 SPAC25B8.14,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC25B8.15c,VIABLE WT cells at 25-32 SPAC25B8.16,ESSENTIAL germinated spores long- some division at 32- microcolonies long cells at 25 SPAC25B8.17,VIABLE WT cells at 25-32 SPAC25B8.18,VIABLE WT cells at 25-32 SPAC25B8.19c,VIABLE WT cells at 25-32 SPAC25G10.01,VIABLE slightly misshapen cells at 25-32 SPAC25G10.02,VIABLE WT cells at 25-32 SPAC25G10.03,VIABLE small colonies WT cells at 25-32 SPAC25G10.04c,VIABLE WT cells at 25-32 SPAC25G10.05c,VIABLE WT cells at 25-32 SPAC25G10.06,VIABLE small colonies WT cells at 25-32 SPAC25G10.07c,ESSENTIAL germinated spores long misshapen at 25-32 SPAC25G10.08,ESSENTIAL spores at 25-32 SPAC25G10.09c,VIABLE WT cells at 25-32 SPAC25H1.02,VIABLE WT cells at 25-32 SPAC25H1.03,VIABLE WT cells at 25-32 SPAC25H1.04,VIABLE WT cells at 25-32 SPAC25H1.05,VIABLE rounded cells at 32- cells slightly misshapen at 25 SPAC25H1.06,VIABLE WT cells at 25-32 SPAC25H1.07,VIABLE slightly misshapen cells at 25-32 SPAC25H1.08c,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPAC25H1.09,VIABLE WT cells at 25-32 SPAC26A3.01,VIABLE WT cells at 25-32 SPAC26A3.02,VIABLE WT cells at 25-32 SPAC26A3.03c,ESSENTIAL microcolonies long cells at 25-32 SPAC26A3.04,VIABLE WT cells at 25-32 SPAC26A3.05,ESSENTIAL spores- germinated spores at 25-32 SPAC26A3.06,VIABLE WT cells at 25-32 SPAC26A3.07c,VIABLE small colonies WT cells at 25-32 SPAC26A3.08,ESSENTIAL spores- germinated spores- occasionally microcolonies wee at 25-32 SPAC26A3.09c,VIABLE long cells at 25-32 SPAC26A3.10,VIABLE WT cells at 25-32 SPAC26A3.11,VIABLE WT cells at 25-32 SPAC26A3.12c,ESSENTIAL spores at 25-32 SPAC26A3.14c,VIABLE WT cells at 25-32 SPAC26A3.15c,ESSENTIAL spores at 25-32 SPAC26A3.16,VIABLE WT cells at 25-32 SPAC26A3.17c,VIABLE WT cells at 25-32 SPAC26F1.01,VIABLE WT cells at 32 SPAC26F1.02,VIABLE WT cells at 25-32 SPAC26F1.03,ESSENTIAL spores- germinated spores at 25-32 SPAC26F1.04c,VIABLE WT cells at 25-32 SPAC26F1.05,VIABLE WT cells at 25-32 SPAC26F1.06,ESSENTIAL spores- germinated spores at 25-32 SPAC26F1.07,VIABLE WT cells at 25-32 SPAC26F1.08c,VIABLE WT cells at 25-32 SPAC26F1.09,VIABLE WT cells at 25-32 SPAC26F1.10c,VIABLE slightly small cells at 25-32 SPAC26F1.12c,VIABLE WT cells at 25-32 SPAC26F1.13c,ESSENTIAL spores- germinated spores at 25-32 SPAC26F1.14c,VIABLE WT cells at 25-32 SPAC26H5.02c,VIABLE WT cells at 25-32 SPAC26H5.03,VIABLE WT cells at 25-32 SPAC26H5.04,VIABLE WT cells at 25-32 SPAC26H5.05,VIABLE small colonies slightly misshapen cells at 25-32 SPAC26H5.06,ESSENTIAL small colonies long cells at 25-32 SPAC26H5.07c,VIABLE WT cells at 25-32 SPAC26H5.08c,VIABLE WT cells at 25-32 SPAC26H5.09c,VIABLE WT cells at 25-32 SPAC26H5.10c,VIABLE WT cells at 32 SPAC26H5.11,VIABLE WT cells at 25-32 SPAC26H5.12,ESSENTIAL microcolonies skittle cells at 25-32 SPAC26H5.13c,ESSENTIAL spores at 25-32 SPAC27D7.02c,VIABLE WT cells at 25-32 SPAC27D7.03c,VIABLE WT cells at 25-32 SPAC27D7.04,VIABLE WT cells at 25-32 SPAC27D7.05c,VIABLE WT cells at 25-32 SPAC27D7.06,VIABLE WT cells at 25-32 SPAC27D7.07c,ESSENTIAL germinated spores at 25-32 SPAC27D7.08c,VIABLE WT cells at 25-32 SPAC27D7.09c,VIABLE WT cells at 25-32 SPAC27D7.11c,VIABLE WT cells at 25-32 SPAC27D7.12c,VIABLE WT cells at 25-32 SPAC27D7.13c,VIABLE WT cells at 25-32 SPAC27D7.14c,VIABLE small colonies long cells at 32- germinated spores long at 25 SPAC27E2.01,VIABLE slightly misshapen cells at 25-32 SPAC27E2.02,VIABLE WT cells at 25-32 SPAC27E2.03c,VIABLE WT cells at 25-32 SPAC27E2.05,ESSENTIAL germinated spores long- some microcolonies long cells at 25-32 SPAC27E2.06c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC27E2.07,VIABLE WT cells at 25-32 SPAC27E2.09,VIABLE WT cells at 32 SPAC27E2.10c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC27E2.11c,VIABLE misshapen septated cells at 32 SPAC27F1.02c,ESSENTIAL germinated spores swollen dumbbell shaped at 25-32 SPAC27F1.03c,VIABLE WT cells at 25-32 SPAC27F1.04c,ESSENTIAL germinated spores- occasionally long- occasionally divide at 25-32 SPAC27F1.05c,VIABLE WT cells at 25-32 SPAC27F1.06c,VIABLE WT cells at 25-32 SPAC27F1.07,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC27F1.08,VIABLE small colonies misshapen cells at 25- cells slightly misshapen at 32 SPAC27F1.09c,ESSENTIAL spores- some germinated spores at 25-32 SPAC27F1.10,VIABLE WT cells at 25-32 SPAC29A4.02c,VIABLE WT cells at 25-32 SPAC29A4.03c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC29A4.04c,ESSENTIAL germinated spores long- some division at 25-32 SPAC29A4.05,VIABLE WT cells at 25-32 SPAC29A4.06c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 32- long cells at 25 SPAC29A4.07,ESSENTIAL microcolonies curved branched septated long cells at 25-32 SPAC29A4.08c,ESSENTIAL germinated spores long at 25-32 SPAC29A4.09,VIABLE WT cells at 25-32 SPAC29A4.10,ESSENTIAL germinated spores- often long and branched at 25-32 SPAC29A4.11,VIABLE WT cells at 25-32 SPAC29A4.12c,VIABLE WT cells at 25-32 SPAC29A4.13,VIABLE WT cells at 25-32 SPAC29A4.14c,VIABLE WT cells at 25-32 SPAC29A4.15,ESSENTIAL spores at 25-32 SPAC29A4.16,VIABLE WT cells at 25-32 SPAC29A4.17c,VIABLE WT cells at 25-32 SPAC29A4.18,VIABLE small colonies long cells at 25-32 SPAC29A4.19c,VIABLE WT cells at 25-32 SPAC29A4.20,VIABLE small colonies long cells at 25-32 SPAC29B12.01,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC29B12.02c,VIABLE WT cells at 25-32 SPAC29B12.03,VIABLE WT cells at 25-32 SPAC29B12.04,VIABLE small colonies WT cells at 25-32 SPAC29B12.05c,VIABLE WT cells at 25-32 SPAC29B12.06c,VIABLE WT cells at 25-32 SPAC29B12.07,ESSENTIAL spores- germinated spores at 25-32 SPAC29B12.08,VIABLE WT cells at 25-32 SPAC29B12.10c,VIABLE WT cells at 25-32 SPAC29B12.11c,VIABLE WT cells at 25-32 SPAC29B12.12,VIABLE WT cells at 25-32 SPAC29B12.13,VIABLE WT cells at 25-32 SPAC29B12.14c,VIABLE WT cells at 25-32 SPAC29E6.01,VIABLE WT cells at 25-32 SPAC29E6.02,ESSENTIAL germinated spores long at 25-32 SPAC29E6.03c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPAC29E6.04,ESSENTIAL germinated spores- microcolonies slightly misshapen cells at 25-32 SPAC29E6.05c,VIABLE WT cells at 25-32 SPAC29E6.06c,ESSENTIAL spores at 25-32 SPAC29E6.07,VIABLE WT cells at 25-32 SPAC29E6.08,ESSENTIAL spores at 25-32 SPAC29E6.09,VIABLE WT cells at 25-32 SPAC29E6.10c,VIABLE WT cells at 25-32 SPAC2C4.03c,ESSENTIAL germinated spores at 25-32 SPAC2C4.04c,ESSENTIAL spores- germinated spores- microcolonies slightly misshapen cells at 25-32 SPAC2C4.05,VIABLE WT cells at 25-32 SPAC2C4.06c,VIABLE WT cells at 25-32 SPAC2C4.07c,VIABLE WT cells at 25-32 SPAC2C4.08,VIABLE WT cells at 25-32 SPAC2C4.09,VIABLE WT cells at 25-32 SPAC2C4.10c,VIABLE WT cells at 25-32 SPAC2C4.11c,ESSENTIAL spores at 25-32 SPAC2C4.12c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC2C4.13,ESSENTIAL spores at 25-32 SPAC2C4.14c,VIABLE WT cells at 25-32 SPAC2C4.15c,VIABLE WT cells at 25-32 SPAC2C4.16c,VIABLE small colonies WT cells at 25-32 SPAC2C4.17c,VIABLE WT cells at 25-32 SPAC2E12.02,ESSENTIAL spores at 25-32 SPAC2E12.03c,VIABLE WT cells at 25-32 SPAC2E1P3.01,VIABLE WT cells at 25-32 SPAC2E1P3.02c,VIABLE WT cells at 25-32 SPAC2E1P3.04,VIABLE WT cells at 25-32 SPAC2E1P3.05c,VIABLE WT cells at 25-32 SPAC2E1P5.01c,VIABLE WT cells at 25-32 SPAC2E1P5.02c,VIABLE WT cells at 25-32 SPAC2E1P5.03,VIABLE WT cells at 25-32 SPAC2E1P5.04c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC2E1P5.05,ESSENTIAL spores at 25-32 SPAC2F3.01,VIABLE WT cells at 25-32 SPAC2F3.02,VIABLE WT cells at 25-32 SPAC2F3.03c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC2F3.04c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC2F3.05c,VIABLE WT cells at 25-32 SPAC2F3.06c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC2F3.07c,VIABLE WT cells at 25-32 SPAC2F3.08,VIABLE WT cells at 25-32 SPAC2F3.09,ESSENTIAL spores- germinated spores slightly misshapen may divide once at 25-32 SPAC2F3.10,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC2F3.11,VIABLE WT cells at 25-32 SPAC2F3.12c,VIABLE slightly curved cells at 32 SPAC2F3.13c,VIABLE curved cells at 25-32 SPAC2F3.14c,ESSENTIAL microcolonies slightly misshapen cells- some long cells- some germinated spores long at 25-32 SPAC2F3.15,VIABLE WT cells at 25-32 SPAC2F3.16,VIABLE WT cells at 25-32 SPAC2F3.17c,ESSENTIAL germinated spores long at 25-32 SPAC2F3.18c,VIABLE WT cells at 25-32 SPAC2F7.02c,VIABLE WT cells at 25-32 SPAC2F7.03c,SUPPRESSED SPAC2F7.04,VIABLE WT cells at 25-32 SPAC2F7.05c,ESSENTIAL germinated spores at 25-32 SPAC2F7.06c,VIABLE WT cells at 25-32 SPAC2F7.07c,VIABLE WT cells at 32 SPAC2F7.08c,VIABLE WT cells at 25-32 SPAC2F7.09c,VIABLE WT cells at 25-32 SPAC2F7.10,VIABLE WT cells at 25-32 SPAC2F7.11,VIABLE WT cells at 25-32 SPAC2F7.13c,ESSENTIAL spores at 25-32 SPAC2F7.14c,ESSENTIAL germinated spores long at 25-32 SPAC2F7.15,ESSENTIAL microcolonies skittle cells at 25-32 SPAC2F7.17,VIABLE WT cells at 25-32 SPAC2G11.02,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC2G11.03c,VIABLE small colonies stubby cells at 25-32 SPAC2G11.04,VIABLE WT cells at 25-32 SPAC2G11.05c,VIABLE WT cells at 25-32 SPAC2G11.06,VIABLE rounded cells at 25-32 SPAC2G11.07c,VIABLE WT cells at 25-32 SPAC2G11.08c,ESSENTIAL microcolonies slightly long curved cells at 25-32 SPAC2G11.09,VIABLE WT cells at 32 SPAC2G11.10c,VIABLE small colonies WT cells with some long cells at 25-32 SPAC2G11.11c,ESSENTIAL microcolonies long cells at 25-32 SPAC2G11.12,VIABLE WT cells with some long cells at 25-32 SPAC2G11.13,VIABLE WT cells at 25-32 SPAC2G11.14,ESSENTIAL spores- germinated spores at 25-32 SPAC2H10.01,VIABLE WT cells at 25-32 SPAC2H10.02c,VIABLE WT cells at 25-32 SPAC30.01c,VIABLE WT cells at 25-32 SPAC30.02c,VIABLE small colonies long cells at 25-32 SPAC30.03c,VIABLE WT cells at 25-32 SPAC30.04c,VIABLE WT cells at 25-32 SPAC30C2.02,VIABLE small colonies WT cells at 25-32 SPAC30C2.03,ESSENTIAL small colonies skittle cells at 25-32 SPAC30C2.04,VIABLE WT cells at 25-32 SPAC30C2.05,VIABLE WT cells at 25-32 SPAC30C2.06c,VIABLE slightly small cells at 32 SPAC30C2.07,VIABLE WT cells at 25-32 SPAC30C2.08,VIABLE WT cells at 25-32 SPAC30D11.01c,VIABLE WT cells at 25-32 SPAC30D11.02c,VIABLE small colonies WT cells at 25-32 SPAC30D11.03,ESSENTIAL germinated spores long at 25-32 SPAC30D11.04c,VIABLE WT cells at 25-32 SPAC30D11.05,VIABLE small colonies WT cells at 25-32 SPAC30D11.06c,VIABLE WT cells at 25-32 SPAC30D11.07,VIABLE WT cells with some long cells at 25-32 SPAC30D11.08c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC30D11.09,VIABLE WT cells at 32 SPAC30D11.10,VIABLE small colonies long cells at 25-32 SPAC30D11.11,VIABLE WT cells at 25-32 SPAC30D11.12,VIABLE WT cells at 25-32 SPAC30D11.13,VIABLE small colonies long cells at 25-32 SPAC30D11.14c,VIABLE WT cells at 25-32 SPAC31A2.02,VIABLE very small colonies rounded cells at 25-32 SPAC31A2.03,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC31A2.04c,ESSENTIAL spores- germinated spores at 25-32 SPAC31A2.05c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC31A2.06,VIABLE WT cells at 25-32 SPAC31A2.07c,ESSENTIAL germinated spores at 25-32 SPAC31A2.08,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC31A2.09c,VIABLE WT cells at 25-32 SPAC31A2.10,VIABLE WT cells at 25-32 SPAC31A2.11c,VIABLE WT cells at 25-32 SPAC31A2.12,VIABLE WT cells at 25-32 SPAC31A2.13c,VIABLE small colonies WT cells at 25- stubby cells at 32 SPAC31A2.14,VIABLE WT cells at 25-32 SPAC31A2.15c,VIABLE WT cells at 25-32 SPAC31A2.16,VIABLE WT cells at 25-32 SPAC31F12.01,VIABLE slightly misshapen cells at 25-32 SPAC31G5.01,ESSENTIAL germinated spores- often long at 25-32 SPAC31G5.02,ESSENTIAL microcolonies rounded cells- colonies appear dark at 25-32 SPAC31G5.03,VIABLE small colonies WT cells at 25-32 SPAC31G5.04,VIABLE slightly misshapen cells at 25-32 SPAC31G5.05c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC31G5.06,ESSENTIAL microcolonies skittle cells at 25-32 SPAC31G5.07,VIABLE WT cells at 25-32 SPAC31G5.08,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC31G5.09c,VIABLE WT cells at 25-32 SPAC31G5.10,VIABLE WT cells at 25-32 SPAC31G5.11,VIABLE WT cells at 25-32 SPAC31G5.12c,VIABLE WT cells at 25-32 SPAC31G5.13,ESSENTIAL germinated spores- occasionally long at 25-32 SPAC31G5.14,VIABLE WT cells at 25-32 SPAC31G5.15,VIABLE WT cells at 25-32 SPAC31G5.16c,ESSENTIAL spores- germinated spores at 25-32 SPAC31G5.17c, VIABLE WT cells at 25-32 SPAC31G5.18c,VIABLE small colonies slightly misshapen cells at 25-32 SPAC31G5.19,VIABLE long cells at 32 SPAC31G5.21,VIABLE WT cells at 25-32 SPAC323.01c,VIABLE small colonies slightly skittle slightly curved cells at 25-32 SPAC323.02c,ESSENTIAL germinated spores at 25-32 SPAC323.03c,VIABLE WT cells at 25-32 SPAC323.04,VIABLE WT cells at 25-32 SPAC323.05c,VIABLE slightly misshapen cells at 32- small colonies rounded cells at 25 SPAC323.06c,ESSENTIAL microcolonies long misshapen cells at 25-32 SPAC323.07c,VIABLE WT cells at 25-32 SPAC323.08,ESSENTIAL microcolonies long cells at 25-32- cells longer at 25 SPAC328.01c,VIABLE WT cells at 25-32 SPAC328.02,VIABLE WT cells at 25-32- many dead cells SPAC328.03,VIABLE WT cells at 25-32 SPAC328.04,VIABLE WT cells at 25-32 SPAC328.05,VIABLE WT cells at 25-32 SPAC328.06,VIABLE WT cells at 25-32 SPAC328.07c,VIABLE WT cells at 25-32 SPAC328.08c,ESSENTIAL microcolonies misshapen slightly long cells- often branched septated at 25-32 SPAC328.09,VIABLE WT cells at 25-32 SPAC328.10c,VIABLE WT cells at 25-32 SPAC32A11.01,VIABLE WT cells at 25-32 SPAC32A11.02c,VIABLE WT cells at 25-32 SPAC32A11.03c,VIABLE WT cells at 25-32 SPAC32A11.04c,ESSENTIAL spores at 25-32 SPAC343.01c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPAC343.02,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC343.03,ESSENTIAL microcolonies long misshapen cells at 25-32 SPAC343.04c,VIABLE WT cells at 25-32 SPAC343.05,ESSENTIAL spores at 25-32 SPAC343.06c,VIABLE WT cells at 25-32 SPAC343.07,VIABLE WT cells at 25-32 SPAC343.08c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC343.09,VIABLE small colonies long cells at 32- cells not so long long at 25 SPAC343.10,VIABLE WT cells at 25-32 SPAC343.11c,VIABLE WT cells at 25-32 SPAC343.12,VIABLE WT cells at 25-32 SPAC343.13,ESSENTIAL microcolonies skittle cells at 25-32 SPAC343.14c,ESSENTIAL spores at 25-32 SPAC343.15,VIABLE WT cells at 25-32 SPAC343.16,VIABLE WT cells at 25-32 SPAC343.17c,ESSENTIAL spores- some germinated spores at 25-32 SPAC343.18,VIABLE WT cells at 25-32 SPAC343.19,VIABLE WT cells at 32 SPAC343.20,VIABLE WT cells at 25-32 SPAC3A11.02,VIABLE WT cells at 25-32 SPAC3A11.03,VIABLE WT cells at 25-32 SPAC3A11.04,VIABLE WT cells at 25-32 SPAC3A11.05c,VIABLE WT cells at 25-32 SPAC3A11.06,VIABLE WT cells at 25-32 SPAC3A11.07,VIABLE WT cells at 25-32 SPAC3A11.08,ESSENTIAL microcolonies long cells at 25-32- curved misshapen cells at 36 SPAC3A11.09,VIABLE WT cells at 25-32 SPAC3A11.10c,VIABLE WT cells at 25-32 SPAC3A11.11c,VIABLE WT cells at 25-32 SPAC3A11.12c,ESSENTIAL germinated spores at 25-32 SPAC3A11.13,VIABLE WT cells at 25-32 SPAC3A11.14c,VIABLE slightly misshapen cells at 25-32 SPAC3A12.02,ESSENTIAL microcolonies skittle cells at 25-32 SPAC3A12.03c,VIABLE WT cells at 25-32 SPAC3A12.04c,ESSENTIAL microcolonies slightly misshapen cells at 25- cells misshapen at 32 SPAC3A12.05c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC3A12.06c,VIABLE WT cells at 25-32 SPAC3A12.07,ESSENTIAL germinated spores long- microcolonies long cells at 25-32 SPAC3A12.08,VIABLE WT cells at 25-32 SPAC3A12.09c,VIABLE WT cells at 25-32 SPAC3A12.10,VIABLE WT cells at 25-32 SPAC3A12.11c,ESSENTIAL germinated spores long at 25-32 SPAC3A12.12,VIABLE WT cells at 25-32 SPAC3A12.13c,VIABLE small colonies WT cells at 25-32 SPAC3A12.14,ESSENTIAL spores at 25-32 SPAC3A12.15,ESSENTIAL microcolonies misshapen septated cells at 25-32 SPAC3A12.16c,ESSENTIAL spores at 25-32 SPAC3A12.17c,VIABLE WT cells at 25-32 SPAC3A12.18,ESSENTIAL spores- some microcolonies slightly misshapen cells at 25-32 SPAC3A12.19,ESSENTIAL small colonies skittle cells at 25-32 SPAC3C7.01c,VIABLE WT cells at 25-32 SPAC3C7.02c,VIABLE WT cells at 25-32 SPAC3C7.03c,VIABLE small colonies long cells at 25-32 SPAC3C7.04,VIABLE WT cells at 25-32 SPAC3C7.05c,VIABLE WT cells at 25-32 SPAC3C7.06c,VIABLE WT cells at 25-32 SPAC3C7.07c,VIABLE WT cells at 25-32 SPAC3C7.08c,VIABLE small colonies long cells at 25-32 SPAC3C7.09,VIABLE WT cells at 25-32 SPAC3C7.10,VIABLE WT cells at 25-32 SPAC3C7.11c,ESSENTIAL small colonies stubby cells at 25-32 SPAC3C7.12,VIABLE slightly curved cells at 25-32- occasionally cells are T-shaped SPAC3C7.13c,VIABLE WT cells at 25-32 SPAC3C7.14c,VIABLE WT cells at 25-32 SPAC3F10.02c,VIABLE WT cells at 25-32 SPAC3F10.03,ESSENTIAL spores at 25-32 SPAC3F10.04,VIABLE WT cells at 25-32 SPAC3F10.05c,VIABLE WT cells- some dead cells at 25-32 SPAC3F10.06c,VIABLE WT cells at 25-32 SPAC3F10.08c,ESSENTIAL germinated spores at 25-32 SPAC3F10.09,VIABLE WT cells at 25-32 SPAC3F10.10c,VIABLE WT cells at 25-32 SPAC3F10.11c,VIABLE WT cells at 25-32 SPAC3F10.12c,VIABLE WT cells at 25-32 SPAC3F10.13,VIABLE WT cells at 25-32 SPAC3F10.15c,VIABLE WT cells at 25-32 SPAC3F10.16c,VIABLE small colonies slightly misshapen cells at 25-32 SPAC3F10.17,VIABLE WT cells at 25-32 SPAC3F10.18c,VIABLE WT cells at 25-32 SPAC3G6.01,VIABLE WT cells at 25-32 SPAC3G6.02,VIABLE small colonies long curved cells at 32- ESSENTIAL microcolonies long cells at 25-36 SPAC3G6.03c,VIABLE WT cells at 25-32 SPAC3G6.04,VIABLE WT cells at 25-32 SPAC3G6.05,VIABLE WT cells at 25-32 SPAC3G6.06c,VIABLE WT cells at 25-32 SPAC3G6.08,ESSENTIAL microcolonies skittle cells at 25-32 SPAC3G6.09c,VIABLE WT cells at 25-32 SPAC3G6.10c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC3G6.11,VIABLE WT cells at 25-32 SPAC3G6.13c,VIABLE WT cells at 25-32 SPAC3G9.01,VIABLE WT cells at 25-32 SPAC3G9.02,VIABLE small colonies slightly long cells at 32- cells slightly misshapen at 25 SPAC3G9.03,VIABLE small colonies WT cells at 25-32 SPAC3G9.04,VIABLE WT cells with some long cells at 25-32- aberrantly septated at 36 SPAC3G9.05,VIABLE WT cells at 25-32 SPAC3G9.06,ESSENTIAL spores at 25-32 SPAC3G9.07c,VIABLE WT cells at 25-32 SPAC3G9.08,VIABLE slightly misshapen cells at 25-32- some colonies misshapen cells and lysis at 36 SPAC3G9.09c,ESSENTIAL spores at 25-32 SPAC3G9.10c,ESSENTIAL spores- germinated spores at 25-32 SPAC3G9.11c,VIABLE WT cells at 25-32 SPAC3G9.12,ESSENTIAL germinated spores misshapen- occasionally long at 25-32 SPAC3G9.13c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC3G9.14,ESSENTIAL germinated spores long at 25-32 SPAC3G9.15c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPAC3G9.16c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC3H1.01c,ESSENTIAL germinated spores- microcolonies slightly misshapen cells- some long cells at 25-32 SPAC3H1.02c,VIABLE WT cells at 25-32 SPAC3H1.03,VIABLE WT cells at 25-32 SPAC3H1.04c,VIABLE WT cells at 25-32 SPAC3H1.05,VIABLE WT cells at 25-32 SPAC3H1.06c,VIABLE slightly misshapen cells at 25-32 SPAC3H1.07,VIABLE WT cells at 25-32 SPAC3H1.08c,VIABLE WT cells at 25-32 SPAC3H1.09c,VIABLE slightly misshapen cells at 25-32 SPAC3H1.10,VIABLE WT cells at 25-32 SPAC3H1.11,VIABLE stubby cells at 25-32 SPAC3H1.12c,VIABLE WT cells at 25-32 SPAC3H1.13,VIABLE WT cells at 25-32 SPAC3H1.14,VIABLE WT cells at 25-32 SPAC3H5.04,VIABLE WT cells at 25-32 SPAC3H5.05c,VIABLE small colonies WT cells at 25-32 SPAC3H5.06c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC3H5.07,VIABLE WT cells at 25-32 SPAC3H5.08c,VIABLE WT cells at 25-32 SPAC3H5.09c,VIABLE WT cells at 32 cells slightly misshapen at 25 SPAC3H5.10,VIABLE slightly misshapen cells at 32- very small colonies slightly misshapen cells at 25 SPAC3H5.11,ESSENTIAL microcolonies long cells at 25-32 SPAC3H5.12c,VIABLE small colonies WT cells at 25-32 SPAC3H8.02,VIABLE WT cells at 25-32 SPAC3H8.03,VIABLE WT cells at 25-32 SPAC3H8.04,VIABLE WT cells at 25-32 SPAC3H8.05c,VIABLE small colonies long cells at 25-32 SPAC3H8.06,ESSENTIAL spores at 32- microcolonies misshapen cells at 25 SPAC3H8.07c,VIABLE small colonies slightly curved cells at 25-32- cells more curved at 36 SPAC3H8.08c,VIABLE WT cells at 25-32 SPAC3H8.09c,VIABLE WT cells at 25-32 SPAC3H8.10,VIABLE small colonies stubby cells at 25-32 SPAC458.02c,VIABLE WT cells at 25-32 SPAC458.03,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC458.04c,VIABLE WT cells at 25-32 SPAC458.05,VIABLE slightly misshapen cells at 25-32 SPAC458.06,VIABLE WT cells at 25-32 SPAC458.07,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC4A8.02c,VIABLE WT cells at 25-32 SPAC4A8.03c,VIABLE WT cells at 25-32 SPAC4A8.04,VIABLE WT cells at 25-32 SPAC4A8.05c,VIABLE slightly misshapen cells at 25-32 SPAC4A8.06c,VIABLE WT cells at 25-32 SPAC4A8.07c,VIABLE WT cells at 25-32 SPAC4A8.08c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC4A8.09c,VIABLE WT cells at 32 SPAC4A8.10,VIABLE WT cells at 25-32 SPAC4A8.11c,ESSENTIAL microcolonies slightly long and wide cells at 25-32 SPAC4A8.12c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC4A8.13c,ESSENTIAL spores at 25-32 SPAC4A8.14,VIABLE WT cells at 25-32 SPAC4A8.15c,ESSENTIAL spores misshapen- swollen often dumbbell shaped at 25-32 SPAC4A8.16c,ESSENTIAL spores- germinated spores at 25-32 SPAC4C5.01,VIABLE WT cells at 25-32 SPAC4C5.02c,VIABLE small colonies slightly misshapen cells at 25- ESSENTIAL microcolonies misshapen cells at 32 SPAC4C5.03,VIABLE WT cells at 25-32 SPAC4C5.04,VIABLE long cells at 25-32 SPAC4D7.01c,VIABLE WT cells at 25-32 SPAC4D7.02c,VIABLE WT cells at 25-32 SPAC4D7.03,VIABLE WT cells at 25-32 SPAC4D7.04c,ESSENTIAL spores- germinated spores at 25-32 SPAC4D7.05,ESSENTIAL spores at 25-32 SPAC4D7.06c,VIABLE WT cells at 25-32 SPAC4D7.07c,VIABLE WT cells at 25-32 SPAC4D7.09,ESSENTIAL spores at 25-32 SPAC4D7.10c,VIABLE small colonies long branched septated cells at 25-32- cells barely grow at 36 SPAC4D7.11,VIABLE WT cells at 32 SPAC4D7.12c,ESSENTIAL germinated spores at 25-32 SPAC4D7.13,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC4F10.02,VIABLE WT cells at 25-32 SPAC4F10.03c,ESSENTIAL microcolonies small cells at 25-32 SPAC4F10.04,VIABLE slightly misshapen cells at 25-32 SPAC4F10.05c,VIABLE small colonies slightly misshapen cells at 32 SPAC4F10.06,VIABLE WT cells at 32 SPAC4F10.07c,VIABLE WT cells at 25-32 SPAC4F10.08,VIABLE WT cells at 25-32 SPAC4F10.09c,ESSENTIAL spores- germinated spores at 25-32 SPAC4F10.10c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC4F10.11,VIABLE long septated cells at 25-32 SPAC4F10.12,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC4F10.13c,VIABLE WT cells at 25-32 SPAC4F10.14c,VIABLE small colonies WT cells at 25-32 SPAC4F10.15c,VIABLE small colonies stubby cells at 25-32 cells grow better at 25 SPAC4F10.16c,VIABLE WT cells at 25-32 SPAC4F10.17,VIABLE WT cells at 25-32 SPAC4F10.18,VIABLE WT cells at 25-32 SPAC4F10.19c,VIABLE WT cells at 25-32 SPAC4F10.20,VIABLE WT cells at 25-32 SPAC4F8.01,VIABLE WT cells at 25-32 SPAC4F8.02c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC4F8.03,VIABLE small colonies stubby curved cells at 32 SPAC4F8.04,ESSENTIAL spores- germinated spores- occasionally microcolonies slightly misshapen at 25-32 SPAC4F8.05c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC4F8.06,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC4F8.07c, ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC4F8.08,VIABLE WT cells at 25-32 SPAC4F8.10c,VIABLE WT cells at 25-32 SPAC4F8.11,VIABLE WT cells at 25-32 SPAC4F8.12c,ESSENTIAL spores- germinated spores at 25-32 SPAC4F8.13c,ESSENTIAL germinated spores long at 25-32 SPAC4F8.14c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPAC4F8.15,VIABLE WT cells at 25-32 SPAC4G8.02c,ESSENTIAL spores at 25-32 SPAC4G8.03c,VIABLE WT cells at 25-32 SPAC4G8.04,VIABLE WT cells at 25-32 SPAC4G8.05,VIABLE WT cells at 25-32 SPAC4G8.06c,VIABLE WT cells at 25-32 SPAC4G8.07c,VIABLE WT cells at 25-32 SPAC4G8.08,VIABLE WT cells at 25-32 SPAC4G8.09,ESSENTIAL microcolonies skittle cells at 25-32 SPAC4G8.10,VIABLE small colonies slightly long cells at 32 SPAC4G8.11c,VIABLE WT cells at 25-32- initially slightly misshapen SPAC4G8.12c,ESSENTIAL germinated spores at 25-32 SPAC4G8.13c,VIABLE WT cells at 25-32 SPAC4G9.02,VIABLE WT cells at 25-32 SPAC4G9.03,ESSENTIAL germinated spores long at 32- spores and germinated spores at 25 SPAC4G9.04c,ESSENTIAL spores at 25-32 SPAC4G9.05,VIABLE WT cells at 25-32 SPAC4G9.06c,VIABLE WT cells at 25-32 SPAC4G9.07,VIABLE WT cells at 25-32 SPAC4G9.08c,ESSENTIAL spores- germinated spores at 25-32 SPAC4G9.09c,VIABLE WT cells at 25-32 SPAC4G9.10,VIABLE WT cells at 25-32 SPAC4G9.11c,VIABLE WT cells at 25-32 SPAC4G9.12,VIABLE WT cells at 25-32 SPAC4G9.13c,VIABLE WT cells at 25-32 SPAC4G9.14,VIABLE WT cells at 25-32 SPAC4G9.15,VIABLE small colonies WT cells at 25-32 SPAC4G9.16c,VIABLE small colonies WT cells at 25-32 SPAC4G9.17c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC4G9.19,VIABLE WT cells at 25-32 SPAC4G9.20c,VIABLE WT cells at 25-32 SPAC4H3.01,VIABLE WT cells at 25-32 SPAC4H3.02c,VIABLE WT cells at 25-32 SPAC4H3.03c,VIABLE WT cells at 25-32 SPAC4H3.04c,VIABLE WT cells at 25-32 SPAC4H3.05,VIABLE WT cells with some long cells at 25-32 SPAC4H3.06,VIABLE WT cells at 25-32 SPAC4H3.07c,VIABLE WT cells at 25-32 SPAC4H3.08,ESSENTIAL spores- germinated spores at 25-32 SPAC4H3.09,ESSENTIAL microcolonies rounded cells at 25-32 SPAC4H3.10c,ESSENTIAL spores at 25-32 SPAC4H3.11c,ESSENTIAL germinated spores long at 25-32 SPAC4H3.13,VIABLE very small colonies long misshapen cells at 32- 25 SPAC4H3.14c,VIABLE WT cells at 25-32 SPAC513.01c,VIABLE WT cells at 25-32 SPAC513.02,VIABLE WT cells at 25-32 SPAC513.03,VIABLE WT cells at 25-32 SPAC513.04,VIABLE WT cells at 25-32 SPAC513.05,VIABLE WT cells at 25-32 SPAC513.06c,VIABLE WT cells at 25-32 SPAC513.07,VIABLE WT cells at 25-32 SPAC521.02,VIABLE WT cells at 25-32 SPAC521.03,VIABLE WT cells at 25-32 SPAC521.04c,VIABLE WT cells at 25-32 SPAC521.05,VIABLE small colonies long cells at 25-32 SPAC56E4.02c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC56E4.03,VIABLE WT cells at 25-32 SPAC56E4.04c,ESSENTIAL spores- germinated spores at 25-32 SPAC56E4.05,ESSENTIAL microcolonies curved cells at 32- cells more WT at 25 SPAC56E4.06c,VIABLE WT cells at 25-32 SPAC56E4.07,VIABLE WT cells at 25-32 SPAC56F8.02,VIABLE WT cells at 25-32 SPAC56F8.03,ESSENTIAL microcolonies long wide cells at 25-32 SPAC56F8.04c,VIABLE WT cells at 25-32 SPAC56F8.05c,VIABLE WT cells at 25-32 SPAC56F8.06c,VIABLE WT cells at 25-32 SPAC56F8.08,VIABLE WT cells at 25-32 SPAC56F8.09,VIABLE WT cells at 25-32 SPAC56F8.10,ESSENTIAL germinated spores at 25-32 SPAC56F8.11,ESSENTIAL germinated spores at 25-32 SPAC56F8.12,VIABLE WT cells at 25-32 SPAC56F8.14c,VIABLE WT cells at 25-32 SPAC56F8.16,VIABLE WT cells at 25-32 SPAC57A10.02,VIABLE small colonies long cells at 25-32 SPAC57A10.03,VIABLE WT cells at 25-32 SPAC57A10.04,VIABLE WT cells at 25-32 SPAC57A10.05c,ESSENTIAL spores at 25-32 SPAC57A10.06,VIABLE WT cells at 25-32 SPAC57A10.07,VIABLE WT cells at 25-32 SPAC57A10.08c,VIABLE WT cells at 25-32 SPAC57A10.09c,VIABLE WT cells at 25-32 SPAC57A10.10c,VIABLE WT cells at 25-32 SPAC57A10.11c,ESSENTIAL microcolonies skittle cells at 25-32 SPAC57A10.12c,VIABLE WT cells at 25-32 SPAC57A10.14,VIABLE WT cells at 25-32 SPAC57A7.04c,VIABLE WT cells at 25-32 SPAC57A7.05,VIABLE WT cells at 25-32 SPAC57A7.06,ESSENTIAL spores- germinated spores at 25-32 SPAC57A7.07c,VIABLE WT cells at 25-32 SPAC57A7.08,VIABLE slightly misshapen cells at 25-32 SPAC57A7.09,VIABLE WT cells at 25-32 SPAC57A7.10c,ESSENTIAL germinated spores at 25-32 SPAC57A7.11,ESSENTIAL germinated spores slightly misshapen- divide once at 25-32 SPAC57A7.12,VIABLE small colonies WT cells at 25-32 SPAC57A7.13,VIABLE WT cells at 25-32 SPAC589.02c,VIABLE WT cells at 25-32 SPAC589.03c,VIABLE small colonies WT cells at 25-32 SPAC589.04,ESSENTIAL microcolonies skittle cells at 25-32 SPAC589.05c,VIABLE WT cells at 32 SPAC589.06c,VIABLE WT cells at 25-32 SPAC589.07c,VIABLE WT cells at 25-32 SPAC589.08c,VIABLE WT cells at 25-32 SPAC589.09,VIABLE WT cells at 25-32 SPAC589.10c,VIABLE WT cells at 25-32 SPAC589.11,VIABLE WT cells at 25-32 SPAC589.12,VIABLE rounded cells at 25-32- possibly ESSENTIAL and gives rise to suppressors SPAC5D6.01,VIABLE WT cells at 25-32 SPAC5D6.02c,VIABLE WT cells at 32 SPAC5D6.04,VIABLE WT cells at 25-32 SPAC5D6.05,VIABLE small colonies long branched septated curved cells at 32 SPAC5D6.06c,VIABLE WT cells at 32 SPAC5D6.07c,VIABLE WT cells at 25-32 SPAC5D6.08c,VIABLE WT cells at 25-32 SPAC5D6.09c,VIABLE WT cells at 25-32 SPAC5D6.10c,VIABLE WT cells at 32 SPAC5D6.12,VIABLE WT cells at 25-32 SPAC5D6.13,VIABLE WT cells at 32 cells slightly misshapen at 25 SPAC5H10.01,VIABLE WT cells at 25-32 SPAC5H10.02c,VIABLE WT cells at 25-32 SPAC5H10.03,VIABLE WT cells at 25-32 SPAC5H10.04,VIABLE WT cells at 25-32 SPAC5H10.05c,VIABLE slightly misshapen cells at 25-32 SPAC5H10.06c,VIABLE WT cells at 25-32 SPAC5H10.07,VIABLE WT cells at 25-32 SPAC5H10.08c,VIABLE WT cells at 25-32 SPAC5H10.09c,VIABLE WT cells at 25-32 SPAC5H10.10,VIABLE WT cells at 25-32 SPAC5H10.11,VIABLE WT cells at 25-32 SPAC5H10.12c,VIABLE WT cells at 32 SPAC5H10.13c,VIABLE WT cells at 25-32 SPAC607.02c,VIABLE slightly misshapen cells at 25-32 SPAC607.03c,ESSENTIAL spores at 25-32 SPAC607.04,ESSENTIAL small colonies rounded cells at 32- larger colonies at 25 SPAC607.05,ESSENTIAL microcolonies rounded cells at 25-32 SPAC607.06c,VIABLE WT cells at 25-32 SPAC607.07c,VIABLE WT cells at 32 SPAC607.08c,VIABLE WT cells at 25-32 SPAC607.09c,VIABLE WT cells at 25-32 SPAC607.10,VIABLE WT cells at 25-32 SPAC630.03,ESSENTIAL swollen spores at 25-32 SPAC630.04c,VIABLE WT cells at 25-32 SPAC630.05,VIABLE WT cells at 25-32 SPAC630.06c,VIABLE WT cells at 25-32 SPAC630.07c,VIABLE WT cells at 25-32 SPAC630.08c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPAC630.09c,VIABLE WT cells at 25-32 SPAC630.10,VIABLE WT cells at 25-32 SPAC630.11,VIABLE WT cells at 25-32 SPAC630.12,ESSENTIAL germinated spores at 25-32 SPAC630.13c,VIABLE slightly misshapen cells at 25-32 SPAC630.14c,VIABLE long cells at 32 SPAC630.15,VIABLE WT cells at 25-32 SPAC631.01c,VIABLE WT cells at 25-32 SPAC631.02,VIABLE small colonies WT cells at 25-32 SPAC637.03,VIABLE WT cells at 25-32 SPAC637.04,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPAC637.05c,ESSENTIAL spores- germinated spores at 25-32 SPAC637.06,VIABLE slightly stubby cells at 25-32 SPAC637.07,VIABLE small colonies slightly curved cells at 25-32- cells more curved at 36 SPAC637.08,ESSENTIAL germinated spores at 25-32 SPAC637.09,VIABLE small colonies WT cells with some long cells at 25-32 SPAC637.10c,VIABLE small colonies WT cells at 25-32 SPAC637.11,VIABLE WT cells at 25-32 SPAC637.12c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAC637.13c,VIABLE WT cells at 25-32 SPAC644.04,ESSENTIAL spores- germinated spores at 25-32 SPAC644.05c,ESSENTIAL germinated spores long at 25-32 SPAC644.06c,VIABLE long cells at 25-32- cells longer at 36 SPAC644.07,VIABLE WT cells at 25-32 SPAC644.08,VIABLE WT cells at 25-32 SPAC644.09,VIABLE WT cells at 25-32 SPAC644.10,ESSENTIAL microcolonies misshapen curved cells at 25-32 SPAC644.11c,VIABLE WT cells at 25-32 SPAC644.12,ESSENTIAL germinated spores long at 25-32 SPAC644.13c,VIABLE WT cells at 25-32 SPAC644.14c,VIABLE long cells at 25-32- cells longer mostly inviable at 36 SPAC644.15,VIABLE small colonies WT cells at 32- cells slightly misshapen at 25 SPAC644.16,ESSENTIAL spores- germinated spores at 25-32 SPAC644.17c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAC644.18c,ESSENTIAL germinated spores slightly misshapen and divide once at 32- microcolonies misshapen cells at 25 SPAC652.01,VIABLE WT cells at 25-32 SPAC664.01c,VIABLE WT cells at 25-32 SPAC664.02c,VIABLE slightly misshapen cells at 25-32 SPAC664.03,VIABLE small colonies WT cells at 25-32 SPAC664.04c,VIABLE WT cells at 25-32 SPAC664.05,ESSENTIAL spores- germinated spores at 25-32 SPAC664.06,ESSENTIAL spores at 25-32 SPAC664.07c,VIABLE WT cells at 25-32 SPAC664.08c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC664.09,ESSENTIAL microcolonies rounded cells at 32 colonies larger at 25 SPAC664.10,VIABLE WT cells at 25-32 SPAC664.11,ESSENTIAL spores at 25-32 SPAC664.12c,VIABLE WT cells at 32 SPAC664.13,VIABLE WT cells at 25-32 SPAC664.14,VIABLE WT cells at 25-32 SPAC664.15,VIABLE WT cells at 25-32 SPAC683.02c,VIABLE WT cells at 25-32 SPAC683.03,VIABLE WT cells at 25-32 SPAC688.02c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC688.03c,VIABLE WT cells at 25-32 SPAC688.04c,VIABLE WT cells at 25-32 SPAC688.06c,VIABLE WT cells at 25-32 SPAC688.07c,VIABLE slightly curved cells at 32- cells slightly misshapen at 25 SPAC688.09,ESSENTIAL microcolonies skittle cells at 25-32 SPAC688.10,VIABLE WT cells at 25-32 SPAC688.11,ESSENTIAL microcolonies stubby- septated cells- some lysis at 25-32 SPAC688.12c,VIABLE WT cells at 25-32 SPAC688.13,VIABLE WT cells at 25-32 SPAC688.14,VIABLE WT cells at 25-32 SPAC694.02,VIABLE WT cells at 25-32 SPAC694.03,VIABLE WT cells at 25-32 SPAC694.04c,VIABLE WT cells at 32 SPAC694.05c,VIABLE WT cells at 25-32 SPAC694.06c,VIABLE WT cells at 25-32 SPAC6B12.02c,VIABLE long cells at 25-32 SPAC6B12.03c,VIABLE slightly misshapen cells at 25-32 SPAC6B12.04c,VIABLE WT cells at 25-32 SPAC6B12.05c,VIABLE WT cells at 25-32 SPAC6B12.06c,VIABLE WT cells at 25-32 SPAC6B12.07c,VIABLE WT cells at 25-32 SPAC6B12.08,VIABLE WT cells at 25-32 SPAC6B12.09,VIABLE WT cells at 25-32- longer at 36 SPAC6B12.10c,ESSENTIAL germinated spores long- some division at 25-32 SPAC6B12.11,ESSENTIAL spores- germinated spores at 25-32 SPAC6B12.12,VIABLE small colonies WT cells at 25-32 SPAC6B12.13,ESSENTIAL spores misshapen at 25-32 SPAC6B12.14c,VIABLE WT cells at 25-32 SPAC6B12.15,VIABLE slightly long cells at 25-32 SPAC6B12.16,VIABLE WT cells at 25-32 SPAC6C3.02c,VIABLE WT cells at 25-32 SPAC6C3.03c,VIABLE WT cells at 25-32 SPAC6C3.04,VIABLE WT cells at 25-32 SPAC6C3.05,VIABLE WT cells at 25-32 SPAC6C3.06c,VIABLE WT cells at 25-32 SPAC6C3.07,VIABLE WT cells at 25-32 SPAC6C3.08,VIABLE slightly misshapen cells at 25-32 SPAC6C3.09,ESSENTIAL spores- germinated spores- often misshapen at 25-32 SPAC6F12.02,VIABLE WT cells at 25-32 SPAC6F12.03c,VIABLE WT cells at 25-32 SPAC6F12.04,VIABLE WT cells at 25-32 SPAC6F12.05c,ESSENTIAL microcolonies rounded cells at 32- cells more WT at 25 SPAC6F12.06,VIABLE slightly stubby cells at 25-32- cells more stubby at 36 SPAC6F12.07,ESSENTIAL microcolonies skittle slightly misshapen cells at 25-32 SPAC6F12.08c,ESSENTIAL spores- germinated spores at 25-32 SPAC6F12.09,VIABLE WT cells occasionally rounded at 25-32 SPAC6F12.10c,VIABLE WT cells at 25-32 SPAC6F12.11c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC6F12.12,VIABLE WT cells at 25-32 SPAC6F12.13c,ESSENTIAL spores at 25-32 SPAC6F12.14,ESSENTIAL germinated spores long- microcolonies slightly misshapen cells- some long cells at 25-32 SPAC6F12.15c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC6F12.16c,ESSENTIAL spores- germinated spores slightly misshapen- occasionally divide at 25-32 SPAC6F12.17,ESSENTIAL spores- germinated spores at 25-32 SPAC6F6.01,VIABLE WT cells at 25-32 SPAC6F6.02c,VIABLE WT cells at 25-32 SPAC6F6.03c,VIABLE WT cells at 25-32 SPAC6F6.04c,VIABLE WT cells at 25-32 SPAC6F6.05,ESSENTIAL germinated spores- occasionally divide at 25-32 SPAC6F6.06c,VIABLE WT cells at 25-32 SPAC6F6.07c,ESSENTIAL spores at 25-32 SPAC6F6.08c,ESSENTIAL spores- germinated spores misshapen at 25-32 SPAC6F6.09,VIABLE slightly misshapen cells at 25-32 SPAC6F6.10c,ESSENTIAL spores- germinated spores at 25-32 SPAC6F6.11c,VIABLE WT cells at 25-32 SPAC6F6.12,VIABLE WT cells at 25-32 SPAC6F6.13c,VIABLE WT cells at 32 SPAC6F6.15,ESSENTIAL microcolonies rounded cells at 25-32 SPAC6F6.16c,ESSENTIAL microcolonies long cells at 25-32 SPAC6F6.17,VIABLE WT cells at 25-32 SPAC6G10.02c,VIABLE WT cells at 25-32 SPAC6G10.03c,VIABLE WT cells at 25-32 SPAC6G10.04c,ESSENTIAL spores- germinated spores at 25-32 SPAC6G10.05c,ESSENTIAL germinated spores slightly misshapen- occasionally long then divide at 25-32 SPAC6G10.06,VIABLE WT cells at 25-32 SPAC6G10.07,ESSENTIAL germinated spores long- some division at 25- microcolonies with some long cells at 32 SPAC6G10.08,VIABLE WT cells at 25-32 SPAC6G10.09,ESSENTIAL small colonies misshapen cells at 25-32 SPAC6G10.10c,VIABLE WT cells at 25-32 SPAC6G10.11c,VIABLE WT cells at 25-32 SPAC6G10.12c,VIABLE slightly long highly branched septated cells at 25-32 SPAC6G9.01c,VIABLE WT cells at 25-32 SPAC6G9.02c,ESSENTIAL germinated spores long- microcolonies with some long cells at 25-32 SPAC6G9.03c,VIABLE WT cells at 25-32 SPAC6G9.04,VIABLE WT cells at 25-32 SPAC6G9.05,VIABLE WT cells at 25-32 SPAC6G9.06c,ESSENTIAL germinated spores misshapen at 25-32 SPAC6G9.07c,ESSENTIAL germinated spores misshapen at 25-32 SPAC6G9.08,VIABLE WT cells at 25-32 SPAC6G9.09c,VIABLE WT cells at 32 SPAC6G9.10c,VIABLE small colonies WT cells at 32- cells WT at 25- very small colonies at 36 SPAC6G9.11,ESSENTIAL spores- germinated spores at 25-32 SPAC6G9.12,VIABLE WT cells at 25-32 SPAC6G9.13c,VIABLE WT cells at 25-32 SPAC6G9.14,VIABLE WT cells at 25-32 SPAC6G9.15c,VIABLE WT cells at 25-32 SPAC6G9.16c,VIABLE WT cells at 25-32 SPAC732.01,ESSENTIAL germinated spores at 25-32 SPAC732.02c,VIABLE WT cells at 25-32 SPAC750.02c,VIABLE WT cells at 25-32 SPAC750.03c,VIABLE WT cells at 25-32 SPAC750.05c,VIABLE small colonies WT cells at 25-32 SPAC750.06c,VIABLE WT cells at 25-32 SPAC750.07c,VIABLE WT cells at 25-32 SPAC750.08c,VIABLE small colonies WT cells at 25-32 SPAC767.01c,VIABLE WT cells at 25-32 SPAC7D4.02c,VIABLE WT cells at 25-32 SPAC7D4.03c,VIABLE WT cells at 32 SPAC7D4.04,VIABLE WT cells at 25-32 SPAC7D4.05,VIABLE WT cells at 25-32 SPAC7D4.06c,VIABLE stubby cells at 25-32 SPAC7D4.07c,VIABLE WT cells at 25-32 SPAC7D4.08,VIABLE WT cells at 25-32 SPAC7D4.09c,ESSENTIAL microcolonies slightly misshapen septated cells at 25-32 SPAC7D4.10,ESSENTIAL spores- germinated spores at 25-32 SPAC7D4.11c,ESSENTIAL spores- germinated spores at 25-32 SPAC7D4.12c,VIABLE slightly misshapen cells at 25-32- cells initially misshapen from germination SPAC7D4.13c,VIABLE WT cells at 25-32 SPAC7D4.14c,VIABLE WT cells at 25-32 SPAC7D4.15c,ESSENTIAL spores- germinated spores- often divide once to give rounded cells at 25-32 SPAC806.02c,ESSENTIAL spores- germinated spores at 25-32 SPAC806.03c,VIABLE WT cells at 25-32 SPAC806.04c,VIABLE WT cells at 25-32 SPAC806.05,ESSENTIAL microcolonies skittle cells at 25-32 -larger colonies at 25 SPAC806.06c,ESSENTIAL microcolonies rounded cells at 25-32 SPAC806.07,VIABLE WT cells at 25-32 SPAC806.08c,VIABLE WT cells at 25-32 SPAC821.03c,VIABLE WT cells at 32 SPAC821.04c,VIABLE WT cells at 25-32 SPAC821.05,VIABLE stubby cells at 25-32 SPAC821.06,VIABLE WT cells at 25-32 SPAC821.07c,VIABLE WT cells at 25-32 SPAC821.08c,ESSENTIAL germinated spores- occasionally microcolonies misshapen cells at 25-32 SPAC821.09,VIABLE slightly misshapen cells at 32- cells more WT at 25 SPAC821.10c,VIABLE WT cells at 25-32 SPAC821.11,VIABLE WT cells at 25-32 SPAC821.12,ESSENTIAL microcolonies rounded cells at 25-32 SPAC821.13c,VIABLE WT cells at 25-32 SPAC823.17,VIABLE WT cells at 25-32 SPAC823.03,VIABLE WT cells at 25-32 SPAC823.04,ESSENTIAL spores- germinated spores slightly misshapen divide twice at 25-32 SPAC823.05c,VIABLE slightly misshapen cells at 25-32 SPAC823.06,ESSENTIAL germinated spores- some division at 25-32 SPAC823.07,ESSENTIAL germinated spores misshapen at 25-32 SPAC823.08c,ESSENTIAL spores at 25-32 SPAC823.09c,VIABLE WT cells at 25-32 SPAC823.10c,VIABLE WT cells at 25-32 SPAC823.11,ESSENTIAL microcolonies long cells at 25-32 SPAC823.12,ESSENTIAL microcolonies misshapen cells at 25-32 SPAC823.13c,VIABLE WT cells at 25-32 SPAC823.14,VIABLE WT cells at 25-32 SPAC823.15,VIABLE WT cells at 32 SPAC823.16c,VIABLE WT cells at 32 SPAC824.02,VIABLE misshapen septated cells at 25-32 SPAC824.03c,VIABLE WT cells at 25-32 SPAC824.04,VIABLE WT cells at 25-32 SPAC824.05,VIABLE small colonies rounded cells at 25-32- no growth at 36 SPAC824.06,ESSENTIAL microcolonies skittle cells at 25-32 SPAC824.07,VIABLE WT cells at 25-32 SPAC824.08,VIABLE WT cells at 25-32 SPAC824.09c,VIABLE WT cells at 25-32 SPAC869.01,VIABLE WT cells at 25-32 SPAC869.02c,VIABLE WT cells at 25-32 SPAC869.03c,VIABLE WT cells at 25-32 SPAC869.04,VIABLE WT cells at 25-32 SPAC869.05c,VIABLE WT cells at 25-32 SPAC869.06c,VIABLE WT cells at 25-32 SPAC869.07c,VIABLE WT cells at 25-32 SPAC869.08,VIABLE WT cells at 25-32 SPAC869.09,VIABLE WT cells at 32 SPAC869.10c,VIABLE WT cells at 25-32 SPAC869.11,VIABLE WT cells at 25-32 SPAC890.02c,VIABLE WT cells at 25-32 SPAC890.03,VIABLE WT cells at 25-32 SPAC890.04c,ESSENTIAL spores- some germinated spores at 25-32 SPAC890.05,VIABLE WT cells at 32 SPAC890.06,VIABLE WT cells at 25-32 SPAC890.07c,VIABLE WT cells at 25-32 SPAC890.08,ESSENTIAL germinated spores at 25-32 SPAC8C9.03,VIABLE WT cells at 25-32 SPAC8C9.04,VIABLE WT cells at 25-32 SPAC8C9.05,VIABLE WT cells at 25-32 SPAC8C9.06c,VIABLE WT cells at 25-32 SPAC8C9.07,VIABLE small colonies slightly misshapen cells at 25- small colonies long vacuolated cells at 32 SPAC8C9.08,VIABLE WT cells at 25-32 SPAC8C9.09c,VIABLE WT cells at 25-32 SPAC8C9.10c,VIABLE small colonies WT cells at 25-32 SPAC8C9.11,VIABLE WT cells at 25-32 SPAC8C9.12c,VIABLE WT cells at 25-32 SPAC8C9.14,VIABLE WT cells at 25-32 SPAC8C9.15c,ESSENTIAL spores at 25-32 SPAC8C9.16c,VIABLE WT cells at 25-32 SPAC8C9.17c,VIABLE WT cells at 25-32 SPAC8C9.19,VIABLE small colonies WT cells at 25-32 SPAC8E11.01c,VIABLE WT cells at 25-32 SPAC8E11.02c,VIABLE stubby cells at 25-32 SPAC8E11.03c,VIABLE WT cells at 25-32 SPAC8E11.04c,VIABLE WT cells at 25-32 SPAC8E11.05c,VIABLE WT cells at 25-32 SPAC8E11.06,VIABLE WT cells at 25-32 SPAC8E11.07c,VIABLE curved slightly long cells at 32 SPAC8E11.10,VIABLE WT cells at 25-32 SPAC8F11.02c,VIABLE small colonies WT cells- some curved at 25-32 SPAC8F11.03,VIABLE WT cells at 25-32 SPAC8F11.04,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPAC8F11.05c,VIABLE WT cells at 25-32 SPAC8F11.06,ESSENTIAL germinated spores long at 25-32 SPAC8F11.07c,ESSENTIAL germinated spores long at 25-32 SPAC8F11.08c,VIABLE WT cells at 25-32 SPAC8F11.09c,VIABLE WT cells at 25-32 SPAC8F11.10c,VIABLE slightly misshapen cells at 25-32 SPAC9.02c,VIABLE WT cells at 25-32 SPAC9.03c,ESSENTIAL spores- some germinated spores at 25-32 SPAC9.05,VIABLE WT cells at 25-32 SPAC9.06c,VIABLE WT cells at 25-32 SPAC9.07c,VIABLE WT cells at 25-32 SPAC9.08c,VIABLE WT cells at 25-32 SPAC9.10,VIABLE WT cells at 25-32 SPAC9.11,VIABLE WT cells at 25-32 SPAC9.12c,VIABLE WT cells at 25-32 SPAC9.13c,VIABLE WT cells at 25-32- possibly poor germination SPAC922.03,VIABLE WT cells at 25-32 SPAC922.04,VIABLE WT cells at 25-32 SPAC922.05c,VIABLE WT cells at 25-32 SPAC922.06,VIABLE WT cells at 25-32 SPAC922.07c,VIABLE WT cells at 25-32 SPAC926.02,VIABLE WT cells at 25-32 SPAC926.03,VIABLE slightly misshapen cells at 25-32 SPAC926.04c,ESSENTIAL spores at 25-32 SPAC926.05c,VIABLE WT cells at 25-32 SPAC926.06c,VIABLE WT cells at 25-32 SPAC926.07c,VIABLE WT cells at 25-32 SPAC926.08c,ESSENTIAL spores- some germinated spores at 25-32 SPAC926.09c,VIABLE WT cells at 25-32 SPAC959.02,ESSENTIAL spores- germinated spores at 25-32 SPAC959.03c,ESSENTIAL spores- germinated spores at 25-32 SPAC959.04c,VIABLE small colonies stubby cells at 25- microcolonies stubby septated cells at 32 SPAC959.05c,VIABLE WT cells at 25-32 SPAC959.06c,VIABLE WT cells at 25-32 SPAC959.07,VIABLE small colonies WT cells at 25-32 SPAC959.08,VIABLE WT cells at 25-32 SPAC959.09c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAC959.10,ESSENTIAL spores- germinated spores at 25-32 SPAC977.01,VIABLE small colonies slightly misshapen cells at 25-32 SPAC977.02,VIABLE WT cells at 25-32 SPAC977.03,VIABLE WT cells at 25-32 SPAC977.05c,VIABLE WT cells at 25-32 SPAC977.06,VIABLE WT cells at 25-32 SPAC977.08,VIABLE WT cells at 25-32 SPAC977.09c,VIABLE WT cells at 25-32 SPAC977.10,VIABLE WT cells at 25-32 SPAC977.11,VIABLE WT cells at 25-32 SPAC977.12,VIABLE WT cells at 25-32 SPAC977.14c,VIABLE WT cells at 25-32 SPAC977.15,VIABLE WT cells at 25-32 SPAC977.16c,VIABLE WT cells at 25-32 SPAC977.17,VIABLE WT cells at 25-32 SPAC9E9.03,VIABLE WT cells at 25-32 SPAC9E9.04,VIABLE WT cells at 25-32 SPAC9E9.05,VIABLE WT cells at 25-32 SPAC9E9.06c,ESSENTIAL spores at 25-32 SPAC9E9.07c,ESSENTIAL germinated spores at 25-32 SPAC9E9.08,VIABLE WT cells at 25-32 SPAC9E9.09c,VIABLE small colonies rounded cells at 25-32 SPAC9E9.10c,VIABLE WT cells at 25-32 SPAC9E9.11,VIABLE WT cells at 32 SPAC9E9.12c,VIABLE WT cells at 25-32 SPAC9E9.13,VIABLE long cells at 25-32 SPAC9E9.14,VIABLE WT cells at 25-32 SPAC9E9.15,VIABLE WT cells at 25-32 SPAC9G1.02,VIABLE WT cells at 25-32 SPAC9G1.03c,VIABLE small colonies slightly misshapen cells at 25-32 SPAC9G1.04,VIABLE WT cells at 25-32 SPAC9G1.05,VIABLE WT cells at 25-32 SPAC9G1.06c,VIABLE slightly misshapen cells at 25-32 SPAC9G1.07,VIABLE slightly misshapen cells at 25-32- cells long misshapen at 36 SPAC9G1.08c,VIABLE WT cells at 25-32 SPAC9G1.09,ESSENTIAL germinated spores long at 25-32 SPAC9G1.10c,VIABLE WT cells at 25-32 SPAC9G1.11c,VIABLE long septated cells- often multi septated at 25-32 SPAC9G1.12,VIABLE WT cells at 25-32 SPAC9G1.13c,ESSENTIAL germinated spores- microcolonies slightly misshapen cells- some long cells at 25-32 SPACUNK12.02c,VIABLE WT cells at 25-32 SPACUNK4.06c,ESSENTIAL spores- germinated spores slightly misshapen- occasionally divide at 25-32 SPACUNK4.08,VIABLE WT cells at 25-32 SPACUNK4.09,VIABLE WT cells at 25-32 SPACUNK4.10,VIABLE WT cells at 25-32 SPACUNK4.11c,VIABLE WT cells at 25-32 SPACUNK4.12c,VIABLE WT cells at 25-32 SPACUNK4.13c,VIABLE WT cells at 25-32 SPACUNK4.14,VIABLE WT cells at 32 SPACUNK4.15,VIABLE WT cells at 25-32 SPACUNK4.16c,VIABLE WT cells at 25-32 SPACUNK4.17,VIABLE WT cells at 32 SPACUNK4.19,VIABLE WT cells at 25-32 SPAP11E10.01,VIABLE WT cells at 32 SPAP11E10.02c,VIABLE WT cells at 25-32 SPAP14E8.02,VIABLE WT cells at 25-32 SPAP14E8.03,ESSENTIAL spores- germinated spores at 25-32 SPAP14E8.04,VIABLE WT cells at 25-32 SPAP14E8.05c,VIABLE WT cells at 25-32 SPAP19A11.05c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPAP27G11.02,VIABLE WT cells at 25-32 SPAP27G11.03,ESSENTIAL microcolonies misshapen cells at 25-32 SPAP27G11.04c,ESSENTIAL microcolonies slightly small cells at 25-32 SPAP27G11.05c,VIABLE small colonies rounded cells at 25-32 SPAP27G11.06c,VIABLE very small colonies slightly misshapen cells at 25-32- microcolonies misshapen cells at 36 SPAP27G11.07c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAP27G11.08c,VIABLE WT cells at 25-32 SPAP27G11.09c,ESSENTIAL spores at 32- spores- occasionally germinated spores long at 25 SPAP27G11.10c,VIABLE very small colonies WT cells with some long cells at 25-32 SPAP27G11.12,VIABLE WT cells at 25-32 SPAP27G11.13c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPAP27G11.14c,VIABLE WT cells at 25-32 SPAP27G11.15,VIABLE WT cells at 25-32 SPAP27G11.16,VIABLE WT cells at 32 SPAP32A8.02,VIABLE WT cells at 25-32 SPAP32A8.03c,VIABLE WT cells at 25-32 SPAP7G5.02c,ESSENTIAL spores- germinated spores at 25-32 SPAP7G5.03,VIABLE WT cells at 25-32 SPAP7G5.04c,VIABLE WT cells at 25-32 SPAP7G5.05,VIABLE WT cells at 25-32 SPAP7G5.06,VIABLE WT cells at 32 SPAP8A3.02c,VIABLE WT cells at 25-32 SPAP8A3.03,VIABLE WT cells at 25-32 SPAP8A3.04c,VIABLE WT cells at 25-32 SPAP8A3.05,VIABLE WT cells at 25-32 SPAP8A3.06,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 32 SPAP8A3.07c,VIABLE small colonies WT cells at 25-32 SPAP8A3.08,ESSENTIAL spores misshapen lysis at 25-32 SPAP8A3.09c,ESSENTIAL spores at 25-32 SPAP8A3.10,ESSENTIAL small colonies skittle cells at 25-32 SPAP8A3.11c,ESSENTIAL microcolonies skittle cells at 25-32 SPAP8A3.12c,VIABLE WT cells at 25-32 SPAP8A3.13c,VIABLE WT cells at 25-32 SPAP8A3.14c,VIABLE WT cells at 25-32 SPAPB17E12.02,VIABLE WT cells at 25-32 SPAPB17E12.03,VIABLE WT cells at 25-32 SPAPB17E12.04c,VIABLE small colonies WT cells at 25-32 SPAPB17E12.05,VIABLE small colonies WT cells at 25-32 SPAPB17E12.07c,ESSENTIAL microcolonies rounded cells at 25-32 SPAPB17E12.08,VIABLE WT cells at 25-32 SPAPB17E12.09,ESSENTIAL microcolonies skittle cells at 25-32 SPAPB17E12.10c,ESSENTIAL microcolonies skittle cells at 25-32 SPAPB17E12.11,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPAPB17E12.12c,VIABLE WT cells at 25-32 SPAPB17E12.13,VIABLE WT cells at 32 SPAPB17E12.14c,VIABLE WT cells at 32 SPAPB18E9.01,VIABLE small colonies WT cells at 25-32 SPAPB18E9.04c,VIABLE slightly misshapen cells at 25-32 SPAPB1A10.02,ESSENTIAL spores- germinated spores at 25-32 SPAPB1A10.03,VIABLE WT cells at 25-32 SPAPB1A10.04c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAPB1A10.05,VIABLE WT cells at 25-32 SPAPB1A10.06c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAPB1A10.07c,VIABLE WT cells at 25-32 SPAPB1A10.08,VIABLE WT cells at 25-32 SPAPB1A10.09,VIABLE WT cells at 25-32 SPAPB1A10.10c,VIABLE WT cells at 25-32 SPAPB1A10.11c,ESSENTIAL microcolonies skittle cells at 25-32 SPAPB1A10.12c,VIABLE WT cells at 25-32- initially cells look stubby SPAPB1A10.13,VIABLE WT cells at 25-32 SPAPB1A10.14,VIABLE WT cells at 25-32 SPAPB1A10.15,VIABLE slightly misshapen cells at 25-32 SPAPB1A11.01,VIABLE WT cells at 25-32 SPAPB1A11.02,VIABLE WT cells at 25-32 SPAPB1A11.03,VIABLE WT cells at 25-32 SPAPB1A11.04c,VIABLE small colonies long cells at 25-32 SPAPB1E7.01c,ESSENTIAL microcolonies long septated misshapen cells at 25- cells wider at 32 SPAPB1E7.02c,VIABLE small colonies long cells- often branched at later times at 25-32 SPAPB1E7.03,ESSENTIAL spores- germinated spores slightly misshapen- divides once at 25-32 SPAPB1E7.04c,VIABLE stubby septated cells at 25-32 SPAPB1E7.05,VIABLE WT cells at 25-32 SPAPB1E7.06c,VIABLE small colonies misshapen cells- often long at 25-32- lots of diploids SPAPB1E7.07,VIABLE WT cells at 32 SPAPB1E7.08c,VIABLE WT cells at 25-32 SPAPB1E7.09,VIABLE WT cells at 25-32 SPAPB1E7.10,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAPB1E7.11c,VIABLE WT cells at 25-32 SPAPB1E7.12,VIABLE WT cells at 25-32 SPAPB21F2.02,VIABLE WT cells at 25-32 SPAPB21F2.03,VIABLE WT cells at 32 SPAPB24D3.01,VIABLE WT cells at 25-32 SPAPB24D3.02c,VIABLE WT cells at 25-32 SPAPB24D3.03,VIABLE WT cells at 25-32 SPAPB24D3.04c,VIABLE WT cells at 25-32 SPAPB24D3.06c,ESSENTIAL microcolonies skittle- rounded cells at 25-32 SPAPB24D3.07c,VIABLE WT cells at 25-32 SPAPB24D3.08c,VIABLE WT cells at 25-32 SPAPB24D3.09c,VIABLE WT cells at 25-32 SPAPB24D3.10c,VIABLE WT cells at 25-32 SPAPB2B4.01c,ESSENTIAL microcolonies misshapen cells at 25-32 SPAPB2B4.02,VIABLE WT cells at 32 SPAPB2B4.03,VIABLE small colonies WT cells at 25-32 SPAPB2B4.04c,VIABLE WT cells at 25-32 SPAPB2B4.05,ESSENTIAL spores- germinated spores- occasionally divide at 25-32 SPAPB2B4.06,VIABLE WT cells at 25-32 SPAPB2B4.07,VIABLE WT cells at 25-32 SPAPB2C8.01,VIABLE WT cells at 25-32 SPAPB8E5.02c,VIABLE WT cells at 25-32 SPAPB8E5.03,VIABLE WT cells at 25-32 SPAPB8E5.04c,VIABLE WT cells at 25-32 SPAPB8E5.05,VIABLE WT cells at 25-32 SPAPB8E5.06c,VIABLE WT cells at 25-32 SPAPB8E5.07c,ESSENTIAL spores at 25-32 SPAPB8E5.08,VIABLE WT cells at 25-32 SPAPB8E5.09,ESSENTIAL spores- germinated spores at 25-32 SPAPB8E5.10,VIABLE WT cells at 25-32 SPAPJ691.02,VIABLE WT cells at 25-32 SPAPJ691.03,VIABLE WT cells at 25-32 SPAPJ695.01c,VIABLE WT cells at 25-32 SPAPJ696.01c,VIABLE WT cells at 25-32 SPAPJ696.02,VIABLE WT cells at 25-32 SPAPJ698.02c,VIABLE WT cells at 25-32 SPAPJ698.03c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPAPJ760.02c,VIABLE WT cells at 25-32 SPAPJ760.03c,VIABLE WT cells at 25-32 SPAPYUG7.02c,VIABLE small colonies long cells at 25-32- occasionally aberrantly septated with misplaced septum SPAPYUG7.03c,VIABLE long septated cells at 25-32- cells more branched at 36 SPAPYUG7.04c,VIABLE WT cells at 25-32 SPAPYUG7.05,ESSENTIAL microcolonies long cells at 25-32 SPAPYUG7.06,VIABLE WT cells at 25-32 SPAPYUK71.03c,VIABLE WT cells at 25-32 SPBC106.01,VIABLE WT cells at 25-32 SPBC106.02c,VIABLE WT cells at 25-32 SPBC106.03,VIABLE WT cells at 25-32 SPBC106.04,VIABLE WT cells at 25-32 SPBC106.05c,VIABLE WT cells at 32 SPBC106.06,ESSENTIAL spores at 25-32 SPBC106.07c,VIABLE very small colonies long cells at 25-32- possibly suppressors SPBC106.08c,VIABLE WT cells at 25-32 SPBC106.09,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC106.10,VIABLE slightly small cells at 25-32 SPBC106.11c,VIABLE WT cells at 25-32 SPBC106.12c,VIABLE WT cells at 25-32 SPBC106.13,VIABLE WT cells at 25-32 SPBC106.14c,ESSENTIAL spores- some germinated spores at 25-32 SPBC106.15,ESSENTIAL spores- germinated spores slightly misshapen divide once at 32 SPBC106.16,VIABLE WT cells at 25-32 SPBC106.17c,VIABLE WT cells at 25-32 SPBC106.18,ESSENTIAL microcolonies slightly rounded cells at 25-32 SPBC106.19,VIABLE WT cells at 25-32 SPBC106.20,VIABLE WT cells at 25-32- cells misshapen- long- septated at 36 SPBC1105.01,VIABLE WT cells at 25-32 SPBC1105.02c,VIABLE WT cells at 25-32 SPBC1105.03c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC1105.04c,VIABLE small colonies WT cells- occasionally T-shaped cells- occasionally long at 25-32 SPBC1105.05,VIABLE WT cells at 25-32 SPBC1105.06,ESSENTIAL microcolonies curved septated cells at 25- 32 SPBC1105.07c,ESSENTIAL microcolonies septated misshapen cells at 32- cells slightly misshapen at 25. SPBC1105.08,VIABLE WT cells at 25-32 SPBC1105.09,VIABLE WT cells at 25-32 SPBC1105.10,VIABLE WT cells at 25-32 SPBC1105.11c,VIABLE WT cells at 25-32 SPBC1105.12,VIABLE WT cells at 25-32 SPBC1105.13c,VIABLE WT cells at 25-32 SPBC1105.14,VIABLE WT cells at 25-32 SPBC1105.15c,VIABLE small colonies slightly misshapen cells at 25-32 SPBC1105.16c,ESSENTIAL microcolonies small cells at 25-32 SPBC1105.17,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC1105.18c,VIABLE WT cells at 32 SPBC115.01c,ESSENTIAL spores- germinated spores long at 25- some division at 32 SPBC115.02c,VIABLE WT cells at 25-32 SPBC115.03,VIABLE WT cells at 25-32 SPBC119.01,ESSENTIAL spores- germinated spores at 25-32 SPBC119.02,ESSENTIAL germinated spores- some small colonies long cells at 25- microcolonies slightly misshapen cells- some long cells at 32 SPBC119.03,VIABLE WT cells at 25-32 SPBC119.04,VIABLE WT cells at 25-32 SPBC119.05c,VIABLE WT cells at 25-32 SPBC119.06,VIABLE WT cells at 25-32 SPBC119.07,VIABLE slightly misshapen cells at 25-32 SPBC119.08,VIABLE slightly misshapen cells at 25-32 SPBC119.09c,ESSENTIAL germinated spores at 25-32 SPBC119.11c,ESSENTIAL microcolonies long cells at 25-32 SPBC119.12,VIABLE slightly misshapen cells at 25-32 SPBC119.13c,ESSENTIAL spores- germinated spores- often long at 25-32 SPBC119.14,VIABLE WT cells at 25-32 SPBC119.15,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC119.16c,VIABLE WT cells at 25-32 SPBC119.17,ESSENTIAL microcolonies skittle cells at 25-32 SPBC119.18,ESSENTIAL microcolonies skittle cells at 25-32 SPBC1198.01,VIABLE WT cells at 25-32 SPBC1198.02,ESSENTIAL germinated spores- occasionally long- some division at 25-32 SPBC1198.03c,VIABLE WT cells at 25-32 SPBC1198.04c,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC1198.05,ESSENTIAL spores- germinated spores- microcolonies rounded cells at 25-32 SPBC1198.06c,VIABLE WT cells at 25-32 SPBC1198.07c,VIABLE WT cells at 25-32 SPBC1198.08,VIABLE WT cells at 32 SPBC1198.09,VIABLE WT cells at 25-32 SPBC1198.10c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC1198.11c,VIABLE small colonies WT cells at 25-32 SPBC1198.12,VIABLE WT cells at 25-32 SPBC1198.13c,ESSENTIAL germinated spores misshapen at 25-32 SPBC1198.14c,VIABLE WT cells at 25-32 SPBC11B10.01,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC11B10.02c,VIABLE WT cells at 25-32 SPBC11B10.03,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC11B10.04c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC11B10.05c,VIABLE WT cells at 25-32- cells slightly curved at 36 SPBC11B10.06,VIABLE WT cells at 25-32 SPBC11B10.07c,VIABLE WT cells at 25-32 SPBC11B10.08,VIABLE WT cells at 25-32 SPBC11B10.09,ESSENTIAL germinated spores long at 25-32 SPBC11B10.10c,VIABLE slightly curved cells at 25-32 SPBC11C11.01,VIABLE WT cells at 25-32 SPBC11C11.02,VIABLE WT cells at 25-32 SPBC11C11.03,ESSENTIAL germinated spores- microcolonies misshapen cells at 25-32 SPBC11C11.04c,ESSENTIAL germinated spores misshapen at 25-32 SPBC11C11.05,ESSENTIAL microcolonies rounded cells at 25-32 SPBC11C11.06c,VIABLE WT cells at 25-32 SPBC11C11.07,VIABLE WT cells at 25-32 SPBC11C11.08,VIABLE WT cells at 25-32 SPBC11C11.09c,VIABLE WT cells at 25-32 SPBC11C11.10,VIABLE WT cells at 25-32 SPBC11C11.11c,VIABLE WT cells at 25-32 SPBC11G11.01,VIABLE WT cells at 25-32 SPBC11G11.02c,VIABLE WT cells at 25-32 SPBC11G11.03,VIABLE WT cells at 25-32 SPBC11G11.04,ESSENTIAL spores- germinated spores slightly misshapen divide once or twice at 25-32 SPBC11G11.05,VIABLE WT cells at 25-32 SPBC11G11.06c,ESSENTIAL spores- germinated spores at 25-32 SPBC11G11.07,ESSENTIAL spores- germinated spores- microcolonies long cells- occasionally misshapen branched at 25-32 SPBC1215.01,VIABLE WT cells at 25-32 SPBC1215.02c,ESSENTIAL microcolonies long branched septated cells at 25-32 SPBC1271.01c,VIABLE WT cells at 25-32 SPBC1271.02,ESSENTIAL germinated spores- some microcolonies misshapen cells at 25-32 SPBC1271.03c,VIABLE WT cells at 25-32 SPBC1271.04c,ESSENTIAL microcolonies slightly misshapen rounded cells at 25-32 SPBC1271.05c,VIABLE WT cells at 25-32 SPBC1271.06c,VIABLE WT cells at 25-32 SPBC1271.07c,VIABLE WT cells at 25-32 SPBC1271.08c,VIABLE WT cells at 25-32 SPBC1271.09,VIABLE WT cells at 25-32 SPBC1271.10c,VIABLE WT cells at 25-32 SPBC1271.11,VIABLE WT cells at 25-32 SPBC1271.12,VIABLE slightly misshapen cells at 25-32 SPBC1271.13,ESSENTIAL microcolonies skittle cells at 25-32 SPBC1271.14,VIABLE WT cells at 25-32 SPBC1271.15c,VIABLE WT cells- microcolonies skittle cells at 25-32 SPBC1289.01c,VIABLE WT cells at 25-32 SPBC1289.02c,ESSENTIAL microcolonies wide- long cells at 25-32 SPBC1289.03c,ESSENTIAL spores- germinated spores at 25-32 SPBC1289.04c,ESSENTIAL microcolonies rounded cells at 25-32 SPBC1289.05c,ESSENTIAL spores- germinated spores at 25-32 SPBC1289.06c,VIABLE WT cells at 25-32 SPBC1289.07c,ESSENTIAL spores- germinated spores misshapen at 25-32 SPBC1289.08,VIABLE WT cells at 25-32 SPBC1289.09,VIABLE WT cells at 25-32 SPBC1289.10c,VIABLE very small colonies rounded cells at 25-32 SPBC1289.11,VIABLE WT cells at 25-32 SPBC1289.12,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC1289.13c,VIABLE WT cells at 25-32 SPBC1289.14,VIABLE WT cells- some long cells at 25-32 SPBC1289.15,VIABLE WT cells at 25-32 SPBC1289.16c,VIABLE WT cells at 25-32 SPBC12C2.01c,VIABLE WT cells at 25-32 SPBC12C2.02c,VIABLE small colonies slightly long cells at 25- small colonies long cells at 32- microcolonies long cells at 36 SPBC12C2.03c,VIABLE WT cells at 25-32 SPBC12C2.04,VIABLE WT cells at 25-32 SPBC12C2.05c,VIABLE WT cells at 25-32 SPBC12C2.06,ESSENTIAL spores at 25-32 SPBC12C2.07c,VIABLE WT cells at 25-32 SPBC12C2.08,VIABLE WT cells at 25-32 SPBC12C2.09c,VIABLE WT cells at 25-32 SPBC12C2.10c,ESSENTIAL germinated spores long at 25-32 SPBC12C2.11,ESSENTIAL germinated spores at 25-32 SPBC12C2.12c,VIABLE WT cells at 25-32 SPBC12C2.13c,VIABLE WT cells at 25-32 SPBC12D12.01,ESSENTIAL microcolonies long cells- some germinated spores 25-32 SPBC12D12.02c,VIABLE WT cells at 25-32 SPBC12D12.03,ESSENTIAL spores at 25-32 SPBC12D12.04c,VIABLE WT cells at 25- rounded cells at 32- no colonies at 36 SPBC12D12.05c,VIABLE WT cells at 25-32 SPBC12D12.06,VIABLE WT cells at 25-32 SPBC12D12.07c,VIABLE WT cells at 25-32 SPBC12D12.08c,ESSENTIAL microcolonies long cells at 25-32 SPBC12D12.09,VIABLE WT cells at 32 SPBC1306.01c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC1306.02,VIABLE small colonies long cells at 25-32 SPBC1347.01c,VIABLE WT cells at 25-32 SPBC1347.02,VIABLE WT cells at 25-32 SPBC1347.03,VIABLE WT cells at 25-32 SPBC1347.04,ESSENTIAL microcolonies skittle- rounded cells at 25-32 SPBC1347.05c,ESSENTIAL microcolonies slightly misshapen cells- lysis at 25-32 SPBC1347.06c,VIABLE WT cells at 25-32 SPBC1347.07,VIABLE WT cells at 32 SPBC1347.08c,VIABLE WT cells at 25-32 SPBC1347.09,VIABLE WT cells at 25-32 SPBC1347.10,ESSENTIAL spores- germinated spores slightly misshapen often divides once at 25-32 SPBC1347.11,VIABLE WT cells at 25-32 SPBC1347.12,VIABLE WT cells at 25-32 SPBC1347.13c,VIABLE WT cells at 25-32 SPBC1348.01,VIABLE WT cells at 25-32 SPBC1348.02,VIABLE WT cells at 25-32 SPBC1348.04,VIABLE WT cells at 25-32 SPBC1348.05,VIABLE WT cells at 25-32 SPBC1348.06c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC1348.07,VIABLE WT cells at 25-32 SPBC1348.08c,VIABLE WT cells at 25-32 SPBC1348.09,VIABLE WT cells at 25-32 SPBC1348.10c,VIABLE WT cells at 25-32 SPBC1348.14c,VIABLE WT cells at 32 SPBC13A2.01c,ESSENTIAL microcolonies long cells at 25-32 SPBC13A2.02,VIABLE WT cells at 25-32 SPBC13A2.03,ESSENTIAL spores- germinated spores misshapen at 25-32 SPBC13A2.04c,VIABLE WT cells at 25-32 SPBC13E7.01,ESSENTIAL spores- some germinated spores at 25-32 SPBC13E7.02,ESSENTIAL spores- germinated spores at 25-32 SPBC13E7.03c,VIABLE WT cells at 25-32 SPBC13E7.04,VIABLE small colonies WT cells at 25-32 SPBC13E7.05,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC13E7.06,VIABLE WT cells at 25-32 SPBC13E7.07,VIABLE WT cells at 25-32 SPBC13E7.08c,VIABLE WT cells at 25-32 SPBC13E7.09,VIABLE WT cells at 25-32 SPBC13E7.10c,ESSENTIAL spores- germinated spores- small colonies long cells at 25-32 SPBC13E7.11,VIABLE WT cells at 25-32 SPBC13G1.01c,ESSENTIAL microcolonies skittle- rounded cells at 25-32 SPBC13G1.02,VIABLE WT cells at 25-32 SPBC13G1.03c,VIABLE WT cells at 25-32 SPBC13G1.04c,VIABLE WT cells at 25-32 SPBC13G1.05,ESSENTIAL spores- germinated spores at 25-32 SPBC13G1.06c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC13G1.07,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC13G1.08c,VIABLE slightly misshapen cells at 25-32 SPBC13G1.09,ESSENTIAL spores at 25-32 SPBC13G1.10c,VIABLE small colonies WT cells at 25-32 SPBC13G1.11,ESSENTIAL spores- germinated spores at 25-32 SPBC13G1.12,VIABLE WT cells at 25-32 SPBC13G1.13,VIABLE WT cells at 25-32 SPBC13G1.14c,VIABLE WT cells at 25-32 SPBC146.01,ESSENTIAL germinated spores at 25-32 SPBC146.02,VIABLE WT cells at 25-32 SPBC146.03c,ESSENTIAL spores- germinated spores- occasionally long- occasionally microcolonies long cells at 25-32 SPBC146.04,VIABLE WT cells at 25-32 SPBC146.05c,ESSENTIAL spores- germinated spores at 25-32 SPBC146.06c,VIABLE WT cells at 25-32 SPBC146.07,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC146.08c,VIABLE very small colonies slightly misshapen cells at 25-32 SPBC146.09c,VIABLE very small colonies misshapen cells at 32 SPBC146.10,VIABLE WT cells at 32 SPBC146.11c,VIABLE WT cells at 25-32 SPBC146.12,VIABLE WT cells at 25-32 SPBC146.13c,VIABLE small colonies stubby cells at 25-32 SPBC146.14c,ESSENTIAL germinated spores- some microcolonies misshapen cells at 25-32 SPBC14C8.01c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPBC14C8.02,ESSENTIAL spores at 25-32 SPBC14C8.03,VIABLE WT cells at 25-32 SPBC14C8.04,VIABLE WT cells at 25-32 SPBC14C8.05c,VIABLE WT cells at 25-32 SPBC14C8.06,ESSENTIAL spores at 25-32 SPBC14C8.07c,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC14C8.09c,VIABLE WT cells at 25-32 SPBC14C8.10,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC14C8.11c,VIABLE WT cells at 25-32 SPBC14C8.12,ESSENTIAL spores- germinated spores- some long curved at 25-32 SPBC14C8.13,VIABLE WT cells at 25-32 SPBC14C8.14c,ESSENTIAL spores- some germinated spores at 25-32 SPBC14C8.15,VIABLE WT cells at 25-32 SPBC14C8.16c,VIABLE WT cells at 25-32 SPBC14C8.17c,VIABLE small colonies branched septated cells at 25-32- ESSENTIAL cells long highly branched at 36 SPBC14F5.02,ESSENTIAL small colonies misshapen cells at 25-32 SPBC14F5.03c,VIABLE slightly misshapen cells at 25-32 SPBC14F5.04c,ESSENTIAL microcolonies long wide cells at 25-32 SPBC14F5.05c,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC14F5.06,ESSENTIAL spores at 25-32 SPBC14F5.07,VIABLE WT cells at 25-32 SPBC14F5.08,ESSENTIAL microcolonies curved septated branched slightly long cells at 25-32 SPBC14F5.09c,VIABLE WT cells at 25-32 SPBC14F5.10c,VIABLE WT cells at 25-32 SPBC14F5.11c,VIABLE WT cells at 25-32 SPBC14F5.12c,VIABLE WT cells at 25-32 SPBC14F5.13c,VIABLE WT cells at 25-32 SPBC1539.01c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC1539.02,VIABLE WT cells at 25-32 SPBC1539.03c,VIABLE WT cells at 25-32 SPBC1539.04,VIABLE WT cells at 25-32 SPBC1539.05,ESSENTIAL germinated spores at 25-32 SPBC1539.06,VIABLE WT cells at 32- cells slightly misshapen at 25 SPBC1539.07c,VIABLE slightly misshapen cells at 25-32 SPBC1539.08,VIABLE WT cells at 32 SPBC1539.09c,ESSENTIAL spores- some germinated spores at 25-32 SPBC1539.10,VIABLE WT cells at 25-32 SPBC15C4.01c,VIABLE WT cells at 25-32 SPBC15C4.02,VIABLE WT cells at 25-32 SPBC15C4.03,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC15C4.04c,VIABLE WT cells at 25-32 SPBC15C4.05,VIABLE WT cells at 25-32 SPBC15C4.06c,VIABLE WT cells at 25-32 SPBC15D4.01c,VIABLE WT cells at 25-32 SPBC15D4.02,VIABLE slightly misshapen cells- initially branched septated slightly long- abnormal colony morphology at 25-32- cells dead at 36. SPBC15D4.03,VIABLE small colonies long cells at 25-32 SPBC15D4.04,ESSENTIAL spores- some germinated spores at 25-32 SPBC15D4.05,VIABLE WT cells at 25-32 SPBC15D4.06,VIABLE WT cells at 32 SPBC15D4.07c,VIABLE WT cells at 25-32 SPBC15D4.09c,VIABLE WT cells at 25-32 SPBC15D4.10c,VIABLE small colonies WT cells at 25-32 SPBC15D4.11c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC15D4.12c,VIABLE WT cells at 25-32 SPBC15D4.13c,VIABLE WT cells at 25-32 SPBC15D4.14,ESSENTIAL microcolonies misshapen septated cells at 25-32 SPBC15D4.15,VIABLE WT cells at 25-32 SPBC1604.01,VIABLE slightly misshapen cells at 25-32 SPBC1604.02c,VIABLE WT cells at 25-32 SPBC1604.03c,VIABLE WT cells at 25- 32 SPBC1604.04,VIABLE WT cells at 25-32 SPBC1604.05,ESSENTIAL germinated spores misshapen at 25-32 SPBC1604.06c,ESSENTIAL germinated spores at 25-32 SPBC1604.07,VIABLE WT cells at 25-32 SPBC1604.08c,VIABLE slightly misshapen vacuolated at 32 SPBC1604.09c,VIABLE WT cells at 32 SPBC1604.10,ESSENTIAL microcolonies long curved septated branched cells at 25-32 SPBC1604.11,VIABLE WT cells at 25-32 SPBC1604.12,VIABLE WT cells at 25-32 SPBC1604.13c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC1604.14c,ESSENTIAL microcolonies rounded cells at 25-32 SPBC1604.15,ESSENTIAL spores- germinated spores at 25-32 SPBC1604.16c,VIABLE WT cells at 25-32 SPBC1604.17c,ESSENTIAL microcolonies rounded cells at 25-32 SPBC1604.18c,VIABLE WT cells at 25-32 SPBC1604.19c,VIABLE WT cells at 25-32 SPBC1604.20c,VIABLE curved slightly long cells- occasionally T-shaped at 25-32 SPBC1604.21c,ESSENTIAL spores at 25-32 SPBC1652.01,VIABLE WT cells at 25-32 SPBC1652.02,VIABLE WT cells at 25-32 SPBC1677.02,ESSENTIAL spores- germinated spores at 25-32 SPBC1677.03c,ESSENTIAL spores- germinated spores- microcolonies skittles cells at 25-32 SPBC1683.01,VIABLE WT cells at 25-32 SPBC1683.02,VIABLE WT cells at 25-32 SPBC1683.03c,VIABLE WT cells at 25-32 SPBC1683.04,VIABLE WT cells at 25-32 SPBC1683.05,VIABLE WT cells at 25-32 SPBC1683.06c,VIABLE WT cells at 25-32 SPBC1683.07,VIABLE WT cells at 25-32 SPBC1683.08,VIABLE WT cells at 25-32 SPBC1683.09c,VIABLE WT cells at 25-32 SPBC1683.10c,VIABLE WT cells at 25-32 SPBC1683.11c,VIABLE WT cells at 25-32 SPBC1683.12,VIABLE WT cells at 25-32 SPBC1683.13c,VIABLE WT cells at 25-32 SPBC1685.01,VIABLE WT cells at 25- slightly misshapen cells at 32- stubby cells at 36 SPBC1685.02c,VIABLE WT cells at 25-32 SPBC1685.03,ESSENTIAL spores at 25-32 SPBC1685.04,VIABLE WT cells at 25-32 SPBC1685.05,VIABLE WT cells at 25-32 SPBC1685.06,VIABLE WT cells at 25-32 SPBC1685.07c,VIABLE WT cells at 25-32 SPBC1685.08,VIABLE WT cells at 25-32 SPBC1685.09,VIABLE WT cells at 25-32 SPBC1685.10,VIABLE WT cells at 32 SPBC1685.11,VIABLE WT cells at 25-32 SPBC1685.13,VIABLE WT cells at 32 SPBC1685.14c,VIABLE WT cells at 25-32 SPBC1685.15c,VIABLE WT cells at 25-32 SPBC1685.16,ESSENTIAL small colonies slightly misshapen cells at 25-32 SPBC16A3.01,VIABLE slightly misshapen cells at 25-32- ESSENTIAL long misshapen cells at 36 SPBC16A3.02c,VIABLE WT cells at 25-32 SPBC16A3.03c,VIABLE WT cells at 25-32 SPBC16A3.04,ESSENTIAL microcolonies rounded skittle cells at 25-32 SPBC16A3.05c,ESSENTIAL spores- germinated spores at 25-32 SPBC16A3.06,VIABLE WT cells at 25-32 SPBC16A3.07c,VIABLE long cells at 32- cells slightly long at 25 SPBC16A3.08c,VIABLE WT cells at 25-32 SPBC16A3.09c,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC16A3.10,VIABLE WT cells at 25-32 SPBC16A3.11,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC16A3.12c,VIABLE WT cells at 25-32 SPBC16A3.13,VIABLE WT cells at 25-32 SPBC16A3.14,VIABLE WT cells at 25-32 SPBC16A3.15c,ESSENTIAL germinated spores misshapen at 25-32 SPBC16A3.16,VIABLE WT cells at 25-32 SPBC16A3.17c,VIABLE WT cells at 25-32 SPBC16A3.18,VIABLE WT cells at 25-32 SPBC16A3.19,VIABLE WT cells at 25-32 SPBC16C6.01c,VIABLE WT cells at 25-32 SPBC16C6.02c,VIABLE WT cells at 25-32 SPBC16C6.03c,VIABLE WT cells at 25-32 SPBC16C6.04,VIABLE small colonies WT cells at 32 SPBC16C6.05,VIABLE WT cells at 25-32 SPBC16C6.06,VIABLE WT cells at 32 SPBC16C6.07c,ESSENTIAL germinated spores at 25-32 SPBC16C6.08c,VIABLE WT cells at 25-32 SPBC16C6.09,VIABLE small colonies rounded cells at 32- cells less rounded after 2 days growth SPBC16C6.10,VIABLE WT cells at 25-32 SPBC16C6.11,VIABLE small colonies WT cells at 25-32 SPBC16C6.12c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC16C6.13c,ESSENTIAL spores- germinated spores at 25-32 SPBC16D10.01c,VIABLE WT cells at 25-32 SPBC16D10.02,VIABLE WT cells at 25-32 SPBC16D10.03,VIABLE WT cells at 25-32 SPBC16D10.04c,ESSENTIAL germinated spores long- often divide at 25-32 SPBC16D10.05,VIABLE WT cells at 25-32 SPBC16D10.06,VIABLE WT cells at 25-32 SPBC16D10.07c,VIABLE WT cells at 25-32 SPBC16D10.08c,VIABLE WT cells at 25-32 SPBC16D10.09,ESSENTIAL germinated spores long at 25-32 SPBC16D10.10,ESSENTIAL microcolonies small cells at 25-32 SPBC16D10.11c,VIABLE WT cells at 25-32 SPBC16E9.01c,VIABLE WT cells at 25-32 SPBC16E9.02c,VIABLE WT cells at 25-32 SPBC16E9.03c,VIABLE WT cells at 25-32 SPBC16E9.05,ESSENTIAL spores- germinated spores- microcolonies slightly misshapen- vacuolated cells at 25-32 SPBC16E9.06c,VIABLE WT cells at 25-32 SPBC16E9.07,VIABLE WT cells at 25-32 SPBC16E9.08,VIABLE WT cells at 25-32 SPBC16E9.09c,VIABLE slightly misshapen cells at 25-32 SPBC16E9.10c,ESSENTIAL spores at 25-32 SPBC16E9.11c,VIABLE WT cells at 25-32 SPBC16E9.12c,VIABLE WT cells at 25-32 SPBC16E9.13,VIABLE WT cells at 25-32 SPBC16E9.14c,VIABLE WT cells at 25-32 SPBC16E9.15,VIABLE WT cells at 25-32 SPBC16E9.16c,VIABLE WT cells at 25-32 SPBC16E9.17c,VIABLE WT cells at 25-32 SPBC16E9.18,VIABLE WT cells at 25-32 SPBC16E9.19,VIABLE WT cells at 25-32 SPBC16G5.01,ESSENTIAL spores- germinated spores- occasionally long at 25-32 SPBC16G5.02c,VIABLE WT cells at 25-32 SPBC16G5.03,VIABLE WT cells at 25-32 SPBC16G5.04,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC16G5.05c,VIABLE WT cells at 25-32 SPBC16G5.06,VIABLE WT cells at 25-32 SPBC16G5.07c,VIABLE WT cells at 25-32 SPBC16G5.08,ESSENTIAL spores- germinated spores slightly misshapen- occasionally divide at 25-32 SPBC16G5.09,VIABLE WT cells at 25-32 SPBC16G5.10,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC16G5.11c,VIABLE WT cells at 25-32 SPBC16G5.12c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC16G5.13,VIABLE WT cells at 25-32 SPBC16G5.14c,ESSENTIAL spores- some germinated spores at 25-32 SPBC16G5.15c,VIABLE WT cells some long cells at 25-32 SPBC16G5.16,VIABLE WT cells at 25-32 SPBC16G5.17,VIABLE WT cells at 25-32 SPBC16G5.18,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC16H5.02,ESSENTIAL spores- germinated spores at 25-32 SPBC16H5.03c,VIABLE slightly misshapen cells- some long at 32 SPBC16H5.04,VIABLE WT cells at 25-32 SPBC16H5.05c,VIABLE WT cells at 25-32 SPBC16H5.06,VIABLE WT cells at 25-32 SPBC16H5.07c,VIABLE small colonies slightly small cells at 25-32 SPBC16H5.08c,VIABLE WT cells at 32- cells slightly misshapen at 25-36 SPBC16H5.09c,VIABLE WT cells at 25-32 SPBC16H5.10c,ESSENTIAL microcolonies long cells at 25-32 SPBC16H5.11c,VIABLE small colonies WT cells at 25-32 SPBC16H5.12c,VIABLE WT cells at 32 SPBC16H5.13,VIABLE WT cells at 25-32 SPBC16H5.15,ESSENTIAL microcolonies long slightly curved cells at 25-32 SPBC1703.01c,ESSENTIAL microcolonies small cells at 25-32 SPBC1703.02,ESSENTIAL microcolonies long cells at 25-32 SPBC1703.03c,VIABLE small colonies WT cells at 25-32 SPBC1703.04,VIABLE WT cells at 25-32 SPBC1703.05,ESSENTIAL spores- germinated spores at 25-32 SPBC1703.06,VIABLE WT cells at 25-32 SPBC1703.07,VIABLE WT cells at 25-32 SPBC1703.08c,VIABLE WT cells at 25-32 SPBC1703.09,VIABLE WT cells at 25-32 SPBC1703.10,ESSENTIAL spores at 25-32 SPBC1703.11,VIABLE WT cells at 25-32 SPBC1703.12,VIABLE WT cells at 25-32 SPBC1703.13c,VIABLE WT cells at 25-32 SPBC1703.14c,VIABLE WT cells at 25-32 SPBC1703.15c,VIABLE very small colonies misshapen cells at 25-32 SPBC1706.01,VIABLE curved cells- some T-shaped- aberrantly septated with septum often misplaced at 25- 32- cells longer at 36 SPBC1706.03,VIABLE slightly misshapen cells at 25-32 SPBC1709.01,VIABLE WT cells at 25-32 SPBC1709.02c,ESSENTIAL spores- some germinated spores at 25-32 SPBC1709.03,VIABLE small colonies slightly misshapen- vacuolated cells at 25-32 SPBC1709.04c,VIABLE WT cells at 25-32 SPBC1709.05,VIABLE WT cells at 25-32 SPBC1709.06,VIABLE WT cells at 25-32 SPBC1709.07,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC1709.08,ESSENTIAL spores- germinated spores at 25-32 SPBC1709.09,VIABLE WT cells at 25-32 SPBC1709.10c,VIABLE WT cells at 25-32 SPBC1709.11c,VIABLE WT cells at 25-32 SPBC1709.12,VIABLE WT cells at 25-32 SPBC1709.13c,VIABLE WT cells at 25-32 SPBC1709.14,VIABLE WT cells at 25-32 SPBC1709.15c,ESSENTIAL spores- germinated spores- microcolonies misshapen cells at 25-32 SPBC1709.16c,VIABLE WT cells at 25-32 SPBC1709.17,ESSENTIAL microcolonies rounded cells at 25-32 SPBC1709.18,VIABLE WT cells at 25-32 SPBC1709.20,ESSENTIAL microcolonies rounded cells at 25-32 SPBC1711.01c,VIABLE WT cells at 25-32 SPBC1711.02,VIABLE WT cells at 25-32 SPBC1711.03,VIABLE WT cells- some dead cells at 25-32 SPBC1711.04,VIABLE WT cells at 25-32 SPBC1711.05,VIABLE WT cells at 25-32 SPBC1711.06,VIABLE WT cells at 25-32 SPBC1711.07,ESSENTIAL germinated spores at 25-32 SPBC1711.08,VIABLE WT cells at 25-32 SPBC1711.09c,VIABLE slightly misshapen cells at 25-32 SPBC1711.10c,ESSENTIAL microcolonies long cells at 25-32 SPBC1711.11,VIABLE WT cells at 25-32 SPBC1711.12,VIABLE WT cells at 25-32 SPBC1711.13,VIABLE WT cells at 25-32 SPBC1711.14,VIABLE WT cells at 25-32 SPBC1711.15c,VIABLE WT cells at 25-32 SPBC1711.16,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC1711.17,ESSENTIAL microcolonies long slightly curved cells- colonies appear dark at 25-32 SPBC1718.01,VIABLE WT cells with some long cells at 25-32 SPBC1718.02,VIABLE WT cells at 25-32 SPBC1718.03,VIABLE very small colonies long cells at 32 SPBC1718.04,ESSENTIAL spores- germinated spores at 25-32 SPBC1718.05,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 32 SPBC1718.06,VIABLE WT cells at 25-32 SPBC1718.07c,VIABLE slightly misshapen small cells at 25-32 SPBC1734.01c,ESSENTIAL spores- germinated spores at 25-32 SPBC1734.02c,ESSENTIAL germinated spores long at 25-32 SPBC1734.03,ESSENTIAL microcolonies rounded cells at 25-32 SPBC1734.04,VIABLE small colonies slightly misshapen cells at 25-32 SPBC1734.05c,VIABLE WT cells at 25-32 SPBC1734.06,VIABLE WT cells with some long cells at 25-32 SPBC1734.07c,VIABLE WT cells at 25-32 SPBC1734.08,VIABLE WT cells at 25-32 SPBC1734.09,VIABLE WT cells at 25-32 SPBC1734.10c,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC1734.11,VIABLE very small colonies slightly curved cells- occasionally T-shaped- often septated at 32 SPBC1734.12c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPBC1734.13,VIABLE small colonies WT cells at 25-32 SPBC1734.14c,ESSENTIAL spores- germinated spores- microcolonies slightly misshapen cells at 25-32 SPBC1734.15,VIABLE WT cells at 25-32 SPBC1734.16c,ESSENTIAL small colonies long cells at 25-32 SPBC1773.01,VIABLE WT cells at 25-32 SPBC1773.02c,VIABLE WT cells at 25-32 SPBC1773.03c,VIABLE WT cells at 25-32 SPBC1773.04,VIABLE slightly misshapen septated at 25-32 SPBC1773.05c,VIABLE WT cells at 25-32 SPBC1773.06c,VIABLE WT cells at 25-32 SPBC1773.07c,ESSENTIAL spores at 25-32 SPBC1773.08c,VIABLE WT cells at 25-32 SPBC1773.09c,VIABLE WT cells at 25-32 SPBC1773.10c,ESSENTIAL spores at 25-32 SPBC1773.11c,ESSENTIAL spores at 25-32 SPBC1773.12,VIABLE WT cells at 25-32 SPBC1773.13,VIABLE WT cells at 25-32 SPBC1773.14,VIABLE WT cells at 25-32 SPBC1773.15,VIABLE WT cells at 25-32 SPBC1773.16c,VIABLE WT cells at 25-32 SPBC1773.17c,VIABLE WT cells at 25-32 SPBC1778.01c,VIABLE slightly misshapen cells at 25-32 SPBC1778.02,VIABLE WT cells at 25-32 SPBC1778.03c,VIABLE WT cells at 25-32 SPBC1778.04,VIABLE WT cells at 25-32 SPBC1778.05c,VIABLE WT cells at 32 SPBC1778.06c,VIABLE very small colonies stubby cells at 25-32 SPBC1778.07,VIABLE WT cells at 25-32 SPBC1778.08c,ESSENTIAL spores misshapen at 25-32 SPBC1778.09,VIABLE WT cells at 25-32 SPBC1778.10c,VIABLE WT cells at 25-32 SPBC17A3.01c,ESSENTIAL microcolonies skittle- rounded cells at 25-32 SPBC17A3.02,VIABLE WT cells at 25-32 SPBC17A3.03c,VIABLE WT cells at 25-32 SPBC17A3.04c,ESSENTIAL spores- germinated spores at 25-32 SPBC17A3.05c,VIABLE small colonies long cells at 25-32 SPBC17A3.06,VIABLE WT cells at 25-32 SPBC17A3.07,ESSENTIAL microcolonies skittle cells at 25-32 SPBC17A3.08,VIABLE WT cells at 25-32 SPBC17A3.09c,VIABLE WT cells at 25-32 SPBC17A3.10,VIABLE WT cells at 25-32 SPBC17D1.01,ESSENTIAL spores at 25-32 SPBC17D1.02,VIABLE WT cells at 25-32 SPBC17D1.03c,ESSENTIAL spores at 25-32 SPBC17D1.04,ESSENTIAL microcolonies long branched septated cells at 25-32 SPBC17D1.05,VIABLE WT cells at 25-32 SPBC17D1.06,VIABLE WT cells at 25-32 SPBC17D1.07c,VIABLE WT cells at 25-32 SPBC17D11.01,VIABLE WT cells at 25-32 SPBC17D11.02c,VIABLE WT cells at 25-32 SPBC17D11.03c,VIABLE WT cells at 25-32 SPBC17D11.04c,VIABLE WT cells at 25-32 SPBC17D11.05,ESSENTIAL spores at 25-32 SPBC17D11.06,ESSENTIAL germinated spores long- often divide at 25-32 SPBC17D11.07c,ESSENTIAL spores- germinated spores at 25-32 SPBC17D11.08,VIABLE WT cells at 25-32 SPBC17F3.01c,VIABLE slightly misshapen cells at 25-32- cells long septated misshapen at 36 SPBC17F3.02,ESSENTIAL microcolonies rounded cells at 25-32 SPBC17G9.02c, VIABLE long cells at 32 SPBC17G9.03c,ESSENTIAL spores at 25-32 SPBC17G9.04c,ESSENTIAL microcolonies slightly misshapen cells- some long cells- often misshapen at 25-32 SPBC17G9.05,VIABLE WT cells at 25-32 SPBC17G9.07,VIABLE small colonies WT cells at 25-32 SPBC17G9.08c,VIABLE WT cells at 25-32 SPBC17G9.09,VIABLE WT cells at 25-32 SPBC17G9.10,VIABLE WT cells at 25-32 SPBC17G9.11c,ESSENTIAL spores- germinated spores- microcolonies skittle cells at 25-32 SPBC17G9.12c,VIABLE WT cells at 25-32 SPBC17G9.13c,ESSENTIAL spores at 25-32 SPBC1815.01,VIABLE WT cells at 25-32 SPBC1826.01c,ESSENTIAL germinated spores at 25-32 SPBC1861.01c,VIABLE small colonies slightly misshapen- vacuolated cells at 25-32- some dead cells SPBC1861.02,VIABLE WT cells at 25-32 SPBC1861.03,VIABLE WT cells at 25-32 SPBC1861.04c,ESSENTIAL germinated spores long- microcolonies long cells at 25-32 SPBC1861.05,VIABLE WT cells at 25- ESSENTIAL microcolonies of skittle cells at 32-36. SPBC1861.06c,VIABLE WT cells at 25-32 SPBC1861.07,VIABLE WT cells at 25-32 SPBC1861.08c,ESSENTIAL microcolonies slightly misshapen cells-occasionally germinated spores long at 25-32 SPBC1861.09,VIABLE WT cells at 25-32 SPBC18A7.01,VIABLE WT cells at 25-32 SPBC18A7.02c,VIABLE WT cells at 25-32 SPBC18E5.01,VIABLE WT cells at 25-32 SPBC18E5.02c,ESSENTIAL spores- germinated spores at 25-32 SPBC18E5.03c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC18E5.04,VIABLE small colonies misshapen cells at 25-32 SPBC18E5.05c,VIABLE small colonies WT cells at 25-32 SPBC18E5.06,ESSENTIAL microcolonies long cells at 25-32 SPBC18E5.07,VIABLE WT cells at 25-32 SPBC18E5.08,VIABLE WT cells at 25-32 SPBC18E5.09c,VIABLE WT cells at 25-32 SPBC18E5.10,VIABLE WT cells at 25-32- many dead cells at 36°C SPBC18E5.11c,VIABLE WT cells at 25-32 SPBC18E5.12c,ESSENTIAL spores- germinated spores at 25-32 SPBC18E5.13,VIABLE WT cells at 25-32 SPBC18E5.14c,VIABLE WT cells at 32 SPBC18H10.02,VIABLE WT cells at 25-32 SPBC18H10.03,ESSENTIAL germinated spores at 25-32 SPBC18H10.04c,VIABLE WT cells at 25-32 SPBC18H10.05,VIABLE WT cells at 25-32 SPBC18H10.06c,VIABLE WT cells at 25-32 SPBC18H10.07,VIABLE WT cells with some long cells at 25-32 SPBC18H10.08c,VIABLE WT cells at 25-32 SPBC18H10.09,VIABLE WT cells at 25-32 SPBC18H10.10c,VIABLE WT cells at 25-32 SPBC18H10.11c,VIABLE WT cells at 25-32 SPBC18H10.12c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC18H10.13,VIABLE WT cells at 25-32 SPBC18H10.14,ESSENTIAL microcolonies long cells at 25-32 SPBC18H10.15,VIABLE WT cells at 25-32 SPBC18H10.16,VIABLE WT cells at 25-32 SPBC18H10.17c,ESSENTIAL small colonies skittle cells at 25-32 SPBC18H10.18c,VIABLE WT cells at 25-32 SPBC18H10.19,VIABLE WT cells at 25-32 SPBC18H10.20c,VIABLE WT cells at 25-32 SPBC1921.01c,VIABLE small colonies WT cells at 25-32 SPBC1921.02,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC1921.03c,VIABLE WT cells at 25-32 SPBC1921.04c,VIABLE WT cells at 25-32 SPBC1921.05,VIABLE WT cells at 25-32 SPBC1921.06c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPBC1921.07c,VIABLE WT cells at 25-32 SPBC19C2.01,ESSENTIAL spores- germinated spores long at 25-32 SPBC19C2.02,VIABLE WT cells at 25-32 SPBC19C2.03,ESSENTIAL germinated spores at 25-32 SPBC19C2.04c,VIABLE WT cells at 25-32 SPBC19C2.05,ESSENTIAL small colonies slightly misshapen cells- sporulating- lysis at 25-32 SPBC19C2.06c,VIABLE WT cells at 25-32 SPBC19C2.07,ESSENTIAL spores at 25-32 SPBC19C2.08,ESSENTIAL microcolonies long cells at 25-32 SPBC19C2.09,VIABLE WT cells at 25-32 SPBC19C2.10,VIABLE WT cells at 25-32 SPBC19C2.11c,ESSENTIAL microcolonies rounded cells at 25-32 SPBC19C2.12,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBC19C2.13c,VIABLE WT cells at 25-32 SPBC19C2.15c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBC19C7.01,VIABLE WT cells at 25-32 SPBC19C7.02,VIABLE rounded cells at 25-32 SPBC19C7.03,VIABLE slightly misshapen cells at 25-32 SPBC19C7.04c,VIABLE WT cells at 25-32 SPBC19C7.05,VIABLE WT cells at 25-32 SPBC19C7.06,ESSENTIAL spores at 25-32 SPBC19C7.07c,ESSENTIAL microcolonies small cells at 25-32 SPBC19C7.08c,VIABLE WT cells at 25-32 SPBC19C7.09c,VIABLE WT cells at 25-32 SPBC19C7.10,VIABLE WT cells at 25-32 SPBC19C7.11,VIABLE WT cells at 25-32 SPBC19C7.12c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPBC19F5.01c,VIABLE WT cells at 25-32 SPBC19F5.02c,ESSENTIAL spores- some germinated spores at 25-32 SPBC19F5.03,ESSENTIAL germinated spores slightly misshapen- often divide once at 25-32 SPBC19F5.04,ESSENTIAL spores at 25-32 SPBC19F5.05c,ESSENTIAL germinated spores at 25-32 SPBC19F8.01c,VIABLE WT cells at 25-32 SPBC19F8.02,VIABLE WT cells at 25-32 SPBC19F8.03c,VIABLE WT cells at 25-32 SPBC19F8.04c,VIABLE WT cells at 25-32 SPBC19F8.05,ESSENTIAL small colonies skittle cells at 25-32 SPBC19F8.06c,VIABLE WT cells at 25-32 SPBC19F8.07,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC19F8.08,VIABLE WT cells at 25-32 SPBC19G7.01c,VIABLE WT cells at 25-32 SPBC19G7.02,VIABLE WT cells at 32 SPBC19G7.03c,VIABLE slightly misshapen cells at 25-32 SPBC19G7.04,VIABLE WT cells at 25-32 SPBC19G7.05c,ESSENTIAL germinated spores very large swollen branched- initially germinated spores long at 25-32 SPBC19G7.06,VIABLE stubby septated cells at 25-32 SPBC19G7.07c,VIABLE WT cells at 25-32 SPBC19G7.08c,VIABLE WT cells- some dead cells at 25-32 SPBC19G7.09,VIABLE small colonies long cells at 25-32- lots of dead cells SPBC19G7.10c,VIABLE slightly misshapen cells at 32 SPBC19G7.13,ESSENTIAL spores- germinated spores- occasionally long at 25-32 SPBC19G7.14c,ESSENTIAL spores- germinated spores slightly misshapen may divide twice at 25-32 SPBC19G7.15,ESSENTIAL microcolonies long cells at 25-32 SPBC19G7.16,VIABLE very small colonies misshapen septated cells- occasionally long at 25-32 SPBC19G7.17,VIABLE very small colonies slightly misshapen cells at 25-32 SPBC19G7.18c,VIABLE WT cells at 25-32 SPBC1A4.01,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC1A4.02c,VIABLE WT cells at 25-32 SPBC1A4.03c,ESSENTIAL germinated spores misshapen at 25-32 SPBC1A4.04,VIABLE WT cells at 25-32 SPBC1A4.05,VIABLE WT cells at 25-32 SPBC1A4.06c,ESSENTIAL microcolonies skittle cells 32- cells more rounded at 25 SPBC1A4.07c,ESSENTIAL microcolonies misshapen cells- some germinated spores long at 25-32 SPBC1A4.08c,ESSENTIAL spores- germinated spores slightly misshapen at 25-32 SPBC1A4.09,VIABLE WT cells at 25-32 SPBC1A4.10c,ESSENTIAL spores- microcolonies misshapen cells at 25-32 SPBC1A4.11c,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC1D7.01,VIABLE slightly misshapen cells at 25-32 SPBC1D7.03,VIABLE slightly misshapen cells at 25-32 SPBC1D7.04,VIABLE slightly curved cells at 32 SPBC1D7.05,VIABLE WT cells at 25-32 SPBC1E8.02,VIABLE WT cells at 25-32 SPBC1E8.03c,VIABLE small colonies WT cells at 25-32 SPBC1E8.05,VIABLE WT cells at 32 SPBC20F10.01,ESSENTIAL microcolonies long cells at 25-32 SPBC20F10.02c,VIABLE WT cells at 25-32 SPBC20F10.03,VIABLE WT cells at 25-32 SPBC20F10.04c,ESSENTIAL microcolonies long cells at 25-32 SPBC20F10.05,VIABLE WT cells at 25-32 SPBC20F10.06,VIABLE WT cells at 25-32 SPBC20F10.07,VIABLE WT cells at 25-32 SPBC20F10.08c,ESSENTIAL microcolonies curved misshapen swollen cells at 25-32 SPBC20F10.09,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC20F10.10,VIABLE WT cells at 25-32 SPBC21.01,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC21.02,VIABLE WT cells at 25-32 SPBC21.03c,VIABLE WT cells at 25-32 SPBC21.04,ESSENTIAL microcolonies curved septated cells at 25-32 SPBC21.05c,VIABLE rounded cells at 25-32 SPBC21.06c,ESSENTIAL germinated spores long- lysis at 25-32 SPBC21.07c,VIABLE WT cells at 25-32 SPBC211.01,ESSENTIAL microcolonies skittle cells at 25-32 SPBC211.02c,ESSENTIAL germinated spores long at 25-32 SPBC211.03c,ESSENTIAL spores- microcolonies misshapen cells at 25-32 SPBC211.04c,ESSENTIAL germinated spores long often divide at 25-32 SPBC211.06,VIABLE WT cells at 25-32 SPBC211.07c,VIABLE WT cells at 25-32 SPBC211.08c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC215.01,VIABLE WT cells at 25-32 SPBC215.02,VIABLE WT cells at 25-32 SPBC215.03c,VIABLE small colonies long cells at 25-32 SPBC215.04,VIABLE WT cells at 32 SPBC215.05,VIABLE long cells at 25- microcolonies long misshapen cells at 32 SPBC215.06c,VIABLE WT cells at 25-32 SPBC215.07c,VIABLE WT cells at 25-32 SPBC215.08c,VIABLE WT cells at 25-32 SPBC215.09c,ESSENTIAL spores at 25-32 SPBC215.10,VIABLE WT cells at 25-32 SPBC215.11c,VIABLE WT cells at 25-32 SPBC215.12,ESSENTIAL germinated spores- often long often divide at 25- 32 SPBC215.13,VIABLE WT cells at 25-32 SPBC215.14c,VIABLE WT cells at 25-32 SPBC216.01c,VIABLE WT cells at 25-32 SPBC216.02,VIABLE WT cells at 32 SPBC216.03,VIABLE WT cells at 25-32 SPBC216.04c,VIABLE WT cells at 25-32 SPBC216.05,VIABLE WT cells at 25-32 SPBC216.06c,VIABLE WT cells at 25-32 SPBC216.07c,ESSENTIAL spores at 25-32 SPBC21B10.02,VIABLE WT cells at 25-32 SPBC21B10.03c,VIABLE WT cells at 25-32 SPBC21B10.04c,VIABLE WT cells at 25-32 SPBC21B10.05c,VIABLE stubby cells at 25- ESSENTIAL microcolonies with some long cells at 32 SPBC21B10.06c,VIABLE WT cells at 25-32 SPBC21B10.07,VIABLE WT cells at 25-32 SPBC21B10.08c,VIABLE WT cells at 25-32 SPBC21B10.09,VIABLE WT cells at 25-32 SPBC21B10.10,VIABLE WT cells at 25-32 SPBC21B10.11,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC21B10.12,VIABLE WT cells at 25-32 SPBC21B10.13c,VIABLE long cells at 25-32 SPBC21C3.01c,VIABLE WT cells at 25-32 SPBC21C3.02c,VIABLE WT cells occasionally long at 25-32 SPBC21C3.03,VIABLE WT cells at 25-32 SPBC21C3.04c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC21C3.05,ESSENTIAL germinated spores long at 25-32 SPBC21C3.06,VIABLE WT cells at 25-32 SPBC21C3.07c,VIABLE curved slightly long cells at 25-32 SPBC21C3.08c,VIABLE WT cells at 25-32 SPBC21C3.09c,VIABLE WT cells at 25-32 SPBC21C3.10c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC21C3.11,VIABLE WT cells at 25-32 SPBC21C3.12c,VIABLE WT cells at 25-32 SPBC21C3.13,VIABLE very small colonies long cells at 25-32 SPBC21C3.14c,VIABLE WT cells at 25-32 SPBC21C3.15c,VIABLE WT cells at 25-32 SPBC21C3.16c,VIABLE WT cells at 25-32 SPBC21C3.17c,VIABLE WT cells at 25-32 SPBC21C3.18,VIABLE WT cells at 25-32 SPBC21C3.19,VIABLE WT cells at 25-32 SPBC21C3.20c,VIABLE WT cells at 25-32 SPBC21D10.05c,ESSENTIAL misshapen spores- microcolonies misshapen cells 25- 32 SPBC21D10.07,VIABLE WT cells at 25-32 SPBC21D10.08c,VIABLE WT cells at 25-32 SPBC21D10.09c,VIABLE WT cells at 25-32 SPBC21D10.10,VIABLE WT cells at 25-32 SPBC21D10.11c,VIABLE WT cells at 25-32 SPBC21D10.12,VIABLE WT cells at 25-32 SPBC21H7.02,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC21H7.03c,VIABLE WT cells at 25-32 SPBC21H7.04,VIABLE small colonies WT cells at 25-32 SPBC21H7.05,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC21H7.06c,VIABLE WT cells at 25-32 SPBC21H7.07c,VIABLE WT cells at 25-32 SPBC23E6.01c,VIABLE WT cells at 25-32 SPBC23E6.02,VIABLE WT cells at 25-32 SPBC23E6.03c,VIABLE WT cells at 25-32 SPBC23E6.04c,ESSENTIAL spores- germinated spores long- some division at 25-32 SPBC23E6.05,VIABLE WT cells at 25-32 SPBC23E6.06c,ESSENTIAL spores at 25-32 SPBC23E6.07c,ESSENTIAL microcolonies long cells at 25-32 SPBC23E6.08,VIABLE slightly misshapen cells at 25-32 SPBC23E6.09,VIABLE WT cells at 25-32 SPBC23E6.10c,VIABLE WT cells at 25-32 SPBC23G7.04c,VIABLE WT cells at 25-32 SPBC23G7.05,ESSENTIAL spores at 25-32 SPBC23G7.06c,VIABLE WT cells at 25-32 SPBC23G7.07c,VIABLE WT cells at 25-32 SPBC23G7.08c,VIABLE WT cells- some dead cells at 32- WT cells at 25 SPBC23G7.10c,VIABLE WT cells at 25-32 SPBC23G7.11,VIABLE WT cells at 25-32 SPBC23G7.12c,VIABLE WT cells at 25-32 SPBC23G7.13c,VIABLE WT cells at 25-32 SPBC23G7.14,VIABLE WT cells at 25-32 SPBC23G7.15c,VIABLE WT cells at 25-32 SPBC23G7.16,VIABLE WT cells at 25-32 SPBC244.01c,ESSENTIAL germinated spores long at 25-32 SPBC244.02c, ESSENTIAL spores- germinated spores- some division at 25-32 SPBC24C6.02,ESSENTIAL spores- germinated spores- small colonies long cells at 32 SPBC24C6.03,ESSENTIAL microcolonies skittle cells at 25-32 SPBC24C6.04,VIABLE WT cells at 25-32 SPBC24C6.05,VIABLE WT cells at 25-32 SPBC24C6.06,VIABLE slightly misshapen cells at 32 SPBC24C6.07,ESSENTIAL germinated spores long- lysis at 25-32 SPBC24C6.08c,VIABLE WT cells at 25-32 SPBC24C6.09c,VIABLE WT cells at 25-32 SPBC24C6.10c,VIABLE WT cells at 25-32 SPBC24C6.11,VIABLE very small colonies long cells at 32- small colonies long cells at 25. SPBC25B2.01,VIABLE WT cells at 25-32 SPBC25B2.02c,VIABLE WT cells at 25-32 SPBC25B2.03,VIABLE WT cells at 25-32 SPBC25B2.04c,VIABLE WT cells at 25-32 SPBC25B2.05,ESSENTIAL spores- germinated spores- occasionally microcolonies slightly misshapen cells- some long cells at 25-32 SPBC25B2.06c,VIABLE WT cells at 25-32 SPBC25B2.07c,VIABLE slightly long cells at 25-32 SPBC25B2.08,VIABLE WT cells at 25-32 SPBC25B2.09c,ESSENTIAL spores- germinated spores at 25-32 SPBC25B2.10,VIABLE WT cells at 25-32 SPBC25B2.11,VIABLE WT cells at 25-32 SPBC25D12.02c,VIABLE WT cells at 25-32 SPBC25D12.03c,ESSENTIAL germinated spores long- microcolonies with some long cells at 25-32 SPBC25D12.04,ESSENTIAL germinated spores long at 25-32 SPBC25D12.05,VIABLE WT cells at 25-32 SPBC25D12.06,VIABLE WT cells at 25-32 SPBC25H2.02,ESSENTIAL spores at 25-32 SPBC25H2.03,VIABLE WT cells at 25-32 SPBC25H2.04c,ESSENTIAL germinated spores- microcolonies rounded skittle cells at 25-32 SPBC25H2.05,VIABLE WT cells at 25-32 SPBC25H2.06c,ESSENTIAL spores- some germinated spores slightly misshapen divide once at 25-32 SPBC25H2.07,ESSENTIAL spores- some germinated spores at 25-32 SPBC25H2.08c,VIABLE WT cells at 25-32 SPBC25H2.09,VIABLE WT cells at 25-32 SPBC25H2.10c,VIABLE WT cells at 25-32 SPBC25H2.11c,VIABLE very small colonies branched- septated slightly long cells at 32 SPBC25H2.12c,ESSENTIAL spores at 25-32 SPBC25H2.13c,ESSENTIAL germinated spores long at 25-32 SPBC25H2.14,VIABLE WT cells at 25-32 SPBC25H2.15,VIABLE WT cells at 25-32 SPBC25H2.16c,VIABLE WT cells at 25-32 SPBC26H8.01,VIABLE WT cells at 25-32 SPBC26H8.02c,ESSENTIAL spores at 25-32 SPBC26H8.03,VIABLE WT cells at 25-32 SPBC26H8.04c,VIABLE very small colonies slightly misshapen cells at 25-32 SPBC26H8.05c,VIABLE WT cells at 25-32 SPBC26H8.06,ESSENTIAL microcolonies small cells- often skittle at 25-32 SPBC26H8.07c,ESSENTIAL germinated spores at 25-32 SPBC26H8.08c, VIABLE very small colonies slightly misshapen cells at 25-32 SPBC26H8.09c,VIABLE WT cells at 25-32 SPBC26H8.10,ESSENTIAL germinated spores long- often divide 25-32 SPBC26H8.11c,VIABLE WT cells at 25-32 SPBC26H8.12,VIABLE WT cells at 25-32 SPBC26H8.13c,VIABLE WT cells at 25-32 SPBC26H8.14c,VIABLE WT cells at 25-32 SPBC27.01c,ESSENTIAL germinated spores at 25-32 SPBC27.02c,VIABLE slightly misshapen cells at 25-32- branched misshapen cells at 36 SPBC27.03,VIABLE WT cells at 25-32 SPBC27.04,VIABLE WT cells at 25-32 SPBC27.06c,VIABLE slightly misshapen cells at 25-32 SPBC27.08c,VIABLE slightly misshapen vacuolated cells at 25-32 SPBC27B12.01c, ESSENTIAL microcolonies stubby cells at 25-32 SPBC27B12.02,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC27B12.03c,VIABLE WT cells at 25-32 SPBC27B12.04c,VIABLE WT cells at 32 SPBC27B12.05,VIABLE WT cells at 25-32 SPBC27B12.06,ESSENTIAL spores- germinated spores divide once at 32- microcolonies misshapen cells at 25 SPBC27B12.07,VIABLE WT cells at 25-32 SPBC27B12.08,VIABLE slightly misshapen cells at 32- possibly supressors- lots of dead cells SPBC27B12.09c,VIABLE very small colonies slightly misshapen cells at 25-32 SPBC27B12.10c,VIABLE small colonies WT cells at 25-32 SPBC27B12.11c,VIABLE WT cells at 25-32 SPBC27B12.12c,ESSENTIAL germinated spores at 25-32 SPBC27B12.13,ESSENTIAL spores at 25-32 SPBC28E12.01c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC28E12.02,VIABLE WT cells at 25-32 SPBC28E12.03,VIABLE slightly misshapen cells at 25-32 SPBC28E12.04,VIABLE WT cells at 25-32 SPBC28E12.05,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC28E12.06c,VIABLE WT cells at 25-32 SPBC28F2.02,VIABLE WT cells at 25-32 SPBC28F2.03,VIABLE WT cells at 25-32 SPBC28F2.04c,ESSENTIAL microcolonies long cells at 25-32 SPBC28F2.05c,VIABLE WT cells at 25-32 SPBC28F2.06c,ESSENTIAL germinated spores at 25-32 SPBC28F2.07,VIABLE WT cells at 25-32 SPBC28F2.08c,VIABLE WT cells at 25-32 SPBC28F2.09,ESSENTIAL germinated spores misshapen at 25-32 SPBC28F2.10c,VIABLE WT cells at 25-32 SPBC28F2.11,VIABLE WT cells at 25-32 SPBC28F2.12,ESSENTIAL spores- germinated spores at 25-32 SPBC29A10.01,VIABLE slightly misshapen cells at 25-32 SPBC29A10.02,VIABLE WT cells at 25-32 SPBC29A10.03c,VIABLE WT cells at 25-32 SPBC29A10.04,ESSENTIAL germinated spores- often long at 25-32 SPBC29A10.05,VIABLE WT cells at 25-32 SPBC29A10.06c,VIABLE WT cells at 25-32 SPBC29A10.07,VIABLE WT cells at 25-32 SPBC29A10.08,VIABLE WT cells at 25-32 SPBC29A10.09c,VIABLE WT cells at 25-32 SPBC29A10.10c,VIABLE WT cells at 25-32 SPBC29A10.11c,VIABLE WT cells at 25-32 SPBC29A10.12,VIABLE WT cells at 25-32 SPBC29A10.13,VIABLE WT cells at 25-32 SPBC29A10.14,VIABLE WT cells at 25-32 SPBC29A10.15,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC29A10.16c,VIABLE WT cells- often dead cells at 25-32 SPBC29A3.01,VIABLE WT cells at 25-32 SPBC29A3.02c,VIABLE WT cells at 25-32 SPBC29A3.03c,VIABLE WT cells at 25-32 SPBC29A3.04,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC29A3.05,VIABLE slightly curved cells at 25-32 SPBC29A3.06,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPBC29A3.07c,VIABLE WT cells at 25-32 SPBC29A3.08,VIABLE WT cells at 32 SPBC29A3.09c,VIABLE WT cells at 25-32 SPBC29A3.10c,VIABLE small colonies WT cells at 25-32 SPBC29A3.11c,VIABLE WT cells at 25-32 SPBC29A3.12,VIABLE small colonies WT cells at 25-32 SPBC29A3.13,VIABLE WT cells at 25-32 SPBC29A3.14c, VIABLE long cells at 25-32 SPBC29A3.15c,ESSENTIAL spores at 32- microcolonies skittle cells at 25 SPBC29A3.16,ESSENTIAL spores- germinated spores at 25-32 SPBC29A3.17,VIABLE WT cells at 25-32 SPBC29A3.18,VIABLE WT cells at 25-32 SPBC29A3.21,VIABLE WT cells at 32 SPBC29B5.01,VIABLE WT cells at 25-32 SPBC29B5.02c,VIABLE WT cells at 25-32 SPBC29B5.03c,VIABLE WT cells at 25-32 SPBC29B5.04c,VIABLE WT cells at 25-32 SPBC2A9.02,VIABLE WT cells at 25-32 SPBC2A9.03,VIABLE WT cells at 25-32 SPBC2A9.04c,VIABLE WT cells at 25-32 SPBC2A9.05c,VIABLE WT cells at 25-32 SPBC2A9.06c,VIABLE WT cells at 32 SPBC2A9.07c,VIABLE WT cells at 25-32 SPBC2A9.08c,ESSENTIAL spores at 25-32 SPBC2A9.09,ESSENTIAL misshapen germinated spores and microcolonies misshapen cells at 32 SPBC2A9.10,ESSENTIAL microcolonies misshapen cells at 32 SPBC2A9.11c,VIABLE WT cells at 25-32 SPBC2A9.12,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC2A9.13,VIABLE WT cells at 25-32 SPBC2D10.03c,VIABLE WT cells at 25-32 SPBC2D10.04,VIABLE WT cells at 25-32 SPBC2D10.05,VIABLE WT cells at 25-32 SPBC2D10.06,VIABLE WT cells at 25-32 SPBC2D10.07c,VIABLE WT cells at 25-32 SPBC2D10.08c,ESSENTIAL microcolonies skittle slightly misshapen cells at 25-32 SPBC2D10.09,VIABLE WT cells at 25-32 SPBC2D10.10c,VIABLE WT cells at 25-32 SPBC2D10.11c,VIABLE WT cells at 25-32 SPBC2D10.12,VIABLE WT cells at 25-32 SPBC2D10.13,VIABLE WT cells at 25-32 SPBC2D10.14c,VIABLE WT cells at 25-32 SPBC2D10.15c,VIABLE WT cells at 25-32 SPBC2D10.16,VIABLE small colonies slightly misshapen- vacuolated cells a -32 SPBC2D10.17,VIABLE WT cells at 25-32 SPBC2D10.18,VIABLE WT cells at 25-32 SPBC2D10.19c,VIABLE WT cells at 25-32 SPBC2D10.20,VIABLE WT cells at 32 SPBC2F12.02c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC2F12.03c,VIABLE slightly long cells at 25-32 SPBC2F12.04,VIABLE WT cells at 32 SPBC2F12.05c,VIABLE WT cells at 25-32 SPBC2F12.07c,VIABLE WT cells at 25-32 SPBC2F12.08c,ESSENTIAL microcolonies stubby- septated cells at 25-32 SPBC2F12.09c,VIABLE WT cells at 32 SPBC2F12.10,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC2F12.11c,VIABLE long cells at 32- microcolonies long cells at 25 SPBC2F12.12c,VIABLE slightly long cells at 32- small colonies long cells at 25 SPBC2F12.13,VIABLE WT cells at 25-32 SPBC2F12.14c,ESSENTIAL germinated spores at 25-32 SPBC2F12.15c,VIABLE slightly misshapen cells at 25-32 SPBC2G2.01c,VIABLE small colonies WT cells at 25-32 SPBC2G2.02,VIABLE WT cells at 25-32 SPBC2G2.03c,VIABLE small colonies misshapen cells possibly reverting to give larger colonies slightly misshapen cells at 32- germinated spores at 25 SPBC2G2.04c,ESSENTIAL microcolonies rounded cells at 25-32 SPBC2G2.05,VIABLE WT cells at 25-32 SPBC2G2.06c,VIABLE WT cells at 25-32 SPBC2G2.07c,VIABLE WT cells at 25-32 SPBC2G2.08,VIABLE WT cells at 25-32 SPBC2G2.09c,VIABLE WT cells at 25-32 SPBC2G2.10c,VIABLE WT cells at 25-32 SPBC2G2.11,ESSENTIAL germinated spores at 25-32 SPBC2G2.12,ESSENTIAL spores- germinated spores at 25-32 SPBC2G2.13c,VIABLE small colonies long cells at 25-32 SPBC2G2.14,VIABLE WT cells at 25-32 SPBC2G2.15c,VIABLE WT cells at 25-32 SPBC2G2.16,ESSENTIAL germinated spores at 25-32 SPBC2G2.17c,VIABLE WT cells at 25-32 SPBC2G5.01,VIABLE WT cells at 25-32 SPBC2G5.02c,VIABLE WT cells at 25-32 SPBC2G5.03,VIABLE WT cells at 25-32- longer at 36 SPBC2G5.04c,VIABLE WT cells at 25-32 SPBC2G5.05,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC2G5.06c,VIABLE WT cells at 25-32 SPBC2G5.07c,ESSENTIAL microcolonies slightly misshapen cells- aberrantly septated to give chains of 4 cells at 25-32 SPBC30B4.01c,VIABLE WT cells at 25-32 SPBC30B4.02c,VIABLE WT cells at 25-32 SPBC30B4.03c,VIABLE slightly misshapen cells at 25-32 SPBC30B4.04c,VIABLE slightly misshapen small cells at 25-32 SPBC30B4.05,ESSENTIAL spores- germinated spores at 25-32 SPBC30B4.06c,VIABLE WT cells at 25-32 SPBC30B4.07c,ESSENTIAL microcolonies long branched septated cells at 25-32 SPBC30B4.08,VIABLE WT cells at 25-32 SPBC30D10.02,ESSENTIAL small colonies long cells at 25-32 SPBC30D10.03c,VIABLE WT cells at 25-32 SPBC30D10.04,VIABLE WT cells at 25-32 SPBC30D10.05c,VIABLE WT cells at 25-32 SPBC30D10.06,ESSENTIAL germinated spores long at 25-32 SPBC30D10.07c,ESSENTIAL small colonies slightly misshapen cells at 25-32 SPBC30D10.08,ESSENTIAL microcolonies skittle cells at 32- cells more rounded at 25 SPBC30D10.09c,VIABLE WT cells at 32 SPBC30D10.10c,VIABLE WT cells at 25-32 SPBC30D10.11,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPBC30D10.12c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC30D10.13c,VIABLE slightly misshapen cells at 25- germinated spores at 32 SPBC30D10.14,VIABLE WT cells at 25-32 SPBC30D10.15,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC30D10.16,VIABLE WT cells at 25-32 SPBC30D10.17c,ESSENTIAL microcolonies rounded cells- lysis at 25- spores at 32 SPBC30D10.18c,VIABLE WT cells at 25-32 SPBC317.01,VIABLE WT cells at 25-32 SPBC31A8.01c,VIABLE WT cells with some long cells at 25-32 SPBC31E1.01c,VIABLE WT cells at 25-32 SPBC31E1.02c,VIABLE slightly misshapen cells at 25-32 SPBC31E1.03,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC31E1.04,ESSENTIAL spores at 25-32 SPBC31E1.05,ESSENTIAL spores- some germinated spores at 25-32 SPBC31E1.06,ESSENTIAL germinated spores at 25-32 SPBC31F10.02,VIABLE WT cells at 25-32 SPBC31F10.03,VIABLE WT cells at 25-32 SPBC31F10.04c,ESSENTIAL small colonies misshapen septated cells at 25-32 SPBC31F10.05,VIABLE WT cells at 25-32 SPBC31F10.06c,ESSENTIAL spores at 25-32 SPBC31F10.07,VIABLE WT cells at 25-32 SPBC31F10.08,VIABLE WT cells at 25-32 SPBC31F10.09c,VIABLE small colonies slightly misshapen- vacuolated cells at 25-32 SPBC31F10.10c,VIABLE rounded cells at 25-32 SPBC31F10.11c,ESSENTIAL germinated spores long at 25-32 SPBC31F10.12,VIABLE WT cells at 25-32 SPBC31F10.13c,VIABLE WT cells at 25-32 SPBC31F10.14c,VIABLE long cells at 25-32 SPBC31F10.15c,VIABLE WT cells at 25-32 SPBC31F10.16,VIABLE WT cells at 25-32 SPBC31F10.17c,VIABLE WT cells at 25-32 SPBC32C12.02,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC32C12.03c,VIABLE WT cells at 25-32 SPBC32F12.01c,VIABLE WT cells at 25-32 SPBC32F12.02,VIABLE WT cells at 25-32 SPBC32F12.03c,VIABLE WT cells at 25-32 SPBC32F12.04,ESSENTIAL germinated spores misshapen- microcolonies with some long cells at 25-32 SPBC32F12.05c,VIABLE small colonies long cells at 25-32 SPBC32F12.06,ESSENTIAL microcolonies slightly curved vacuolated cells at 25-32 SPBC32F12.07c,VIABLE WT cells at 25-32 SPBC32F12.08c,VIABLE slightly misshapen cells at 25-32 SPBC32F12.09,VIABLE WT cells at 25-32 SPBC32F12.10,ESSENTIAL germinated spores at 25-32 SPBC32F12.11,VIABLE small colonies long cells at 25-32 SPBC32F12.12c,VIABLE WT cells at 25-32 SPBC32H8.01c,VIABLE WT cells at 25-32 SPBC32H8.02c,VIABLE WT cells at 25-32 SPBC32H8.03,VIABLE WT cells at 25-32 SPBC32H8.04c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPBC32H8.05,VIABLE WT cells at 25-32 SPBC32H8.06,VIABLE WT cells at 25-32 SPBC32H8.07,VIABLE WT cells at 25-32 SPBC32H8.08c,VIABLE WT cells at 25-32 SPBC32H8.09,VIABLE small colonies WT cells at 25-32 SPBC32H8.10,ESSENTIAL spores- germinated spores at 25-32 SPBC32H8.11,VIABLE WT cells at 25-32 SPBC32H8.12c,ESSENTIAL spores at 25-32 SPBC32H8.13c,VIABLE WT cells at 25-32 SPBC336.01,VIABLE long cells at 25-32 SPBC336.02,ESSENTIAL germinated spores at 25-32 SPBC336.03,VIABLE rounded cells at 25-32 SPBC336.04,ESSENTIAL germinated spores long at 25-32 SPBC336.05c,VIABLE WT cells at 25-32 SPBC336.06c,VIABLE WT cells at 25-32 SPBC336.07,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC336.08,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC336.09c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC336.10c,VIABLE WT cells at 25-32 SPBC336.11,ESSENTIAL small colonies misshapen cells at 25-32 SPBC336.12c,ESSENTIAL germinated spores long at 25-32 SPBC336.13c,VIABLE WT cells at 25-32 SPBC336.14c,VIABLE slightly misshapen cells at 25-32 SPBC336.15,ESSENTIAL germinated spores long at 25-32 SPBC337.03,VIABLE WT cells at 25-32 SPBC337.04,VIABLE WT cells at 25-32 SPBC337.05c,ESSENTIAL spores at 25-32 SPBC337.06c,ESSENTIAL microcolonies long cells at 25-32 SPBC337.07c,VIABLE WT cells at 25-32 SPBC337.08c,VIABLE WT cells at 25-32 SPBC337.09,VIABLE WT cells at 25-32 SPBC337.10c,VIABLE WT cells at 25-32 SPBC337.11,VIABLE WT cells at 25-32 SPBC337.12,ESSENTIAL spores- germinated spores- microcolonies misshapen cells at 25-32 SPBC337.13c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC337.14,ESSENTIAL misshapen germinated spores- microcolonies misshapen septated cells at 25-32 SPBC337.15c,VIABLE WT cells at 25-32 SPBC337.16,VIABLE WT cells at 25-32 SPBC342.01c,VIABLE stubby cells at 25-32 SPBC342.02,ESSENTIAL germinated spores at 25-32 SPBC342.03,VIABLE WT cells at 25-32 SPBC342.04,VIABLE WT cells at 25-32 SPBC342.05,VIABLE WT cells at 25-32 SPBC342.06c,VIABLE WT cells at 25-32 SPBC354.01,VIABLE WT cells at 25-32 SPBC354.02c,ESSENTIAL spores at 25-32 SPBC354.03,VIABLE long cells at 25-32 SPBC354.04,VIABLE WT cells at 25-32 SPBC354.05c,VIABLE WT cells at 25-32 SPBC354.06,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC354.07c,VIABLE WT cells at 25-32 SPBC354.08c,VIABLE WT cells at 25-32 SPBC354.09c,VIABLE WT cells at 25-32 SPBC354.10,VIABLE WT cells at 25-32 SPBC354.12,VIABLE WT cells at 25-32 SPBC354.13,VIABLE WT cells at 25-32- initially stubby cells SPBC354.14c,VIABLE WT cells at 25-32 SPBC354.15,VIABLE WT cells at 25-32 SPBC359.01,VIABLE WT cells at 25-32 SPBC359.02,ESSENTIAL germinated spores at 25-32 SPBC359.03c,VIABLE WT cells at 25-32 SPBC359.04c,VIABLE WT cells at 25-32 SPBC359.05,VIABLE WT cells at 25-32 SPBC359.06,VIABLE WT cells at 25-32 SPBC36.01c,VIABLE WT cells at 25-32 SPBC36.02c,VIABLE WT cells at 25-32 SPBC36.03c,VIABLE WT cells at 25-32 SPBC36.04,VIABLE small colonies WT cells at 25-32 SPBC36.05c,ESSENTIAL small colonies long cells at 25-32 SPBC36.06c,VIABLE WT cells at 25-32 SPBC36.07,VIABLE long cells at 25-32 SPBC36.08c,ESSENTIAL spores- germinated spores at 25-32 SPBC36.09,ESSENTIAL germinated spores long at 25-32 SPBC36.10,VIABLE WT cells at 25-32 SPBC36.11,VIABLE WT cells at 25-32 SPBC36.12c,ESSENTIAL microcolonies long cells- lysis at 25-32 SPBC365.01,VIABLE WT cells at 25-32 SPBC365.02c,VIABLE WT cells at 25-32 SPBC365.03c,VIABLE small colonies long cells at 25-32 SPBC365.04c,VIABLE WT cells at 25-32 SPBC365.06,VIABLE very small colonies long cells at 25-32 SPBC365.07c,VIABLE WT cells at 25-32 SPBC365.08c,VIABLE WT cells at 25-32 SPBC365.09c,ESSENTIAL spores- germinated spores at 25-32 SPBC365.10,VIABLE very small colonies slightly misshapen cells at 25-32 SPBC365.11,VIABLE WT cells at 25-32 SPBC365.12c,VIABLE WT cells at 25-32 SPBC365.13c,VIABLE WT cells at 32 SPBC365.14c,VIABLE small colonies stubby cells at 25-32- lots of dead cells SPBC365.15,ESSENTIAL microcolonies curved misshapen cells- colonies have a dark edge at 25-32 SPBC365.16,VIABLE WT cells at 25-32 SPBC365.20c,VIABLE WT cells at 25-32 SPBC36B7.02,VIABLE WT cells at 25-32 SPBC36B7.03,VIABLE WT cells at 25-32 SPBC36B7.04,VIABLE WT cells at 25-32 SPBC36B7.05c,VIABLE WT cells at 25-32 SPBC36B7.06c,VIABLE WT cells at 25-32 SPBC36B7.07,ESSENTIAL spores at 25-32 SPBC36B7.08c,VIABLE WT cells at 25-32 SPBC36B7.09,ESSENTIAL spores at 25-32 SPBC3B8.01c,ESSENTIAL microcolonies small cells at 25-32 SPBC3B8.02,VIABLE small colonies long cells at 25-32 SPBC3B8.03,VIABLE WT cells at 25-32 SPBC3B8.04c,VIABLE WT cells at 25-32 SPBC3B8.05,VIABLE WT cells at 25-32 SPBC3B8.06,VIABLE WT cells at 25-32 SPBC3B8.07c,VIABLE WT cells at 25-32 SPBC3B8.08,VIABLE WT cells at 25-32 SPBC3B8.09,ESSENTIAL spores- germinated spores at 25-32 SPBC3B8.10c,VIABLE small colonies WT cells with some long cells at 32 SPBC3B8.11,ESSENTIAL germinated spores long- some division at 25-32 SPBC3B9.01,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC3B9.02c,ESSENTIAL spores- germinated spores at 25-32 SPBC3B9.03,ESSENTIAL spores at 25-32 SPBC3B9.04,VIABLE WT cells at 25-32 SPBC3B9.05,VIABLE WT cells at 25-32 SPBC3B9.06c,VIABLE WT cells at 25-32 SPBC3B9.07c,ESSENTIAL germinated spores long- often divide 25-32 SPBC3B9.08c,VIABLE WT cells at 25-32 SPBC3B9.09,VIABLE WT cells at 25-32 SPBC3B9.10,ESSENTIAL germinated spores at 25-32 SPBC3B9.11c,VIABLE WT cells at 25-32 SPBC3B9.12,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC3B9.13c,VIABLE slightly misshapen cells at 25-32 SPBC3B9.14c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC3B9.15c,VIABLE WT cells at 32 SPBC3B9.16c,ESSENTIAL small colonies slightly misshapen cells at 25-32 SPBC3B9.17,ESSENTIAL spores- some germinated spores at 25-32 SPBC3B9.18c,ESSENTIAL spores- germinated spores at 25-32 SPBC3B9.19,ESSENTIAL swollen spores at 25-32 some germinated spores at 25 SPBC3B9.21,ESSENTIAL microcolonies long cells at 25-32- grows better at 25 SPBC3D6.02,VIABLE WT cells at 25-32 SPBC3D6.03c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC3D6.04c,VIABLE WT cells with some long cells at 25-32 SPBC3D6.05,VIABLE WT cells at 25-32 SPBC3D6.06c,VIABLE WT cells at 25-32 SPBC3D6.07,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC3D6.08c,VIABLE long cells at 32- cells not long but often curved at 25 SPBC3D6.09,VIABLE WT cells at 25-32 SPBC3D6.10,VIABLE WT cells at 25-32 SPBC3D6.11c,ESSENTIAL microcolonies long cells at 25-32 SPBC3D6.12,ESSENTIAL microcolonies long cells at 25-32 SPBC3D6.13c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPBC3D6.15,VIABLE WT cells at 25-32 SPBC3E7.01,VIABLE WT cells at 25-32 SPBC3E7.02c,VIABLE WT cells at 25-32 SPBC3E7.05c,VIABLE WT cells at 25-32 SPBC3E7.06c,VIABLE WT cells at 25-32 SPBC3E7.07c,VIABLE WT cells at 25-32 SPBC3E7.08c,VIABLE WT cells at 25-32 SPBC3E7.09,VIABLE slightly misshapen cells at 25-32 SPBC3E7.10,VIABLE WT cells at 25-32 SPBC3E7.11c,VIABLE WT cells at 25-32 SPBC3E7.12c,VIABLE WT cells at 25-32 SPBC3E7.13c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBC3E7.14,ESSENTIAL germinated spores at 25-32 SPBC3E7.15c,VIABLE small colonies stubby cells at 25-32 SPBC3E7.16c,VIABLE WT cells at 25-32 SPBC3F6.01c,VIABLE WT cells at 25-32 SPBC3F6.02c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPBC3F6.03,VIABLE WT cells at 32 SPBC3F6.04c,ESSENTIAL microcolonies long cells at 25-32 SPBC3F6.05,VIABLE WT cells at 25-32 SPBC3H7.01,ESSENTIAL spores at 25-32 SPBC3H7.02,VIABLE WT cells at 25-32 SPBC3H7.03c,VIABLE slightly misshapen cells at 25-32 SPBC3H7.04,ESSENTIAL microcolonies skittle cells at 25-32 SPBC3H7.05c,VIABLE WT cells at 25-32 SPBC3H7.06c,VIABLE WT cells at 25-32 SPBC3H7.07c,VIABLE WT cells at 25-32 SPBC3H7.08c,VIABLE WT cells at 25-32 SPBC3H7.09,VIABLE WT cells at 25-32 SPBC3H7.10,VIABLE small colonies long cells at 25-32 SPBC3H7.11,VIABLE WT cells at 25-32 SPBC3H7.12,VIABLE WT cells at 25-32 SPBC3H7.13,VIABLE WT cells at 25-32 SPBC3H7.14,VIABLE WT cells at 25-32 SPBC3H7.15,VIABLE WT cells at 25- slightly long cells at 32 SPBC4.01,VIABLE WT cells at 25-32 SPBC4.02c,VIABLE WT cells at 25-32 SPBC4.03c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC4.04c,ESSENTIAL germinated spores- often long at 25-32 SPBC4.05,VIABLE WT cells at 25-32 SPBC4.06,VIABLE WT cells at 25-32 SPBC4.07c,ESSENTIAL germinated spores at 25-32 SPBC405.01,VIABLE WT cells at 25-32 SPBC405.02c,VIABLE WT cells at 25-32 SPBC405.03c,VIABLE WT cells at 25-32 SPBC405.04c,VIABLE WT cells at 25-32 SPBC405.05,VIABLE WT cells at 25-32 SPBC405.06,VIABLE WT cells at 25-32 SPBC405.07,VIABLE WT cells at 25-32 SPBC409.04c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC409.05,ESSENTIAL spores at 25-32 SPBC409.06,VIABLE WT cells at 25-32 SPBC409.07c,VIABLE long cells at 25-32 SPBC409.08,VIABLE WT cells at 25-32 SPBC409.09c,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC409.10,VIABLE WT cells at 25-32 SPBC409.11,VIABLE WT cells at 32 SPBC409.12c,ESSENTIAL microcolonies long cells at 25-32 SPBC409.13,ESSENTIAL germinated spores at 25-32 SPBC409.14c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC409.15,ESSENTIAL spores- germinated spores at 25-32 SPBC409.16c,VIABLE WT cells at 25-32 SPBC409.17c,VIABLE WT cells at 25-32 SPBC409.18,VIABLE WT cells at 25-32 SPBC409.19c,VIABLE slightly misshapen cells at 25-32- rounded cells at 36 SPBC409.20c,VIABLE WT cells at 25-32 SPBC409.21,ESSENTIAL microcolonies stubby cells- occasionally long at 25-32 SPBC418.01c,VIABLE WT cells at 25-32 SPBC418.02,VIABLE WT cells at 25-32 SPBC428.01c,ESSENTIAL germinated spores long- microcolonies slightly misshapen cells- some long cells at 25-32 SPBC428.02c,VIABLE WT cells at 25-32 SPBC428.03c,VIABLE long cells at 25-32 SPBC428.04,VIABLE WT cells at 25-32 SPBC428.05c,VIABLE WT cells at 25-32 SPBC428.06c,VIABLE small colonies long cells at 25-32 SPBC428.07,VIABLE WT cells at 25-32 SPBC428.08c,VIABLE WT cells at 25-32 SPBC428.10,VIABLE WT cells at 25-32 SPBC428.11,VIABLE WT cells at 32 SPBC428.12c,VIABLE WT cells at 25-32 SPBC428.13c,ESSENTIAL germinated spores long- lysis at 25-32 SPBC428.14,VIABLE WT cells at 25-32 SPBC428.15,VIABLE WT cells at 25-32 SPBC428.16c,ESSENTIAL spores at 25-32 SPBC428.17c,VIABLE WT cells at 25-32 SPBC428.18,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC428.19c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC428.20c,ESSENTIAL germinated spores long often misshapen at 25-32 SPBC4B4.01c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC4B4.02c,VIABLE WT cells at 25-32 SPBC4B4.03,VIABLE small colonies long cells at 25-32 SPBC4B4.04,VIABLE WT cells at 25-32 SPBC4B4.05,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC4B4.06,VIABLE WT cells at 25-32 SPBC4B4.07c,VIABLE small colonies long misshapen cells at 25-32 SPBC4B4.08,VIABLE WT cells at 25-32 SPBC4B4.09,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC4B4.10c,VIABLE WT cells at 25-32 SPBC4B4.11,VIABLE WT cells at 25-32 SPBC4B4.12c,VIABLE WT cells at 25-32 SPBC4C3.03,ESSENTIAL spores- some germinated spores at 25-32 SPBC4C3.04c,VIABLE WT cells at 25-32 SPBC4C3.05c,ESSENTIAL spores at 25-32 SPBC4C3.06,VIABLE WT cells at 25-32 SPBC4C3.07,ESSENTIAL microcolonies long cells at 25-32 SPBC4C3.08,VIABLE WT cells at 25-32 SPBC4C3.09,VIABLE WT cells at 25-32 SPBC4C3.10c,ESSENTIAL spores- germinated spores at 25-32 SPBC4C3.12,VIABLE long branched septated- often curved at 25-32 SPBC4F6.04,VIABLE WT cells at 25-32 SPBC4F6.05c,VIABLE WT cells at 25-32 SPBC4F6.06,VIABLE small colonies misshapen- septated cells at 25-32 SPBC4F6.07c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC4F6.08c,VIABLE WT cells at 25-32 SPBC4F6.09,VIABLE WT cells at 25-32 SPBC4F6.10,VIABLE WT cells at 25-32 SPBC4F6.11c,VIABLE WT cells at 25-32 SPBC4F6.12,VIABLE slightly misshapen cells at 32- more septated cells at 25 SPBC4F6.13c,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPBC4F6.14,ESSENTIAL germinated spores misshapen at 25-32 SPBC4F6.15c,VIABLE long cells at 25-32 SPBC4F6.16c,VIABLE WT cells at 25-32 SPBC4F6.17c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC4F6.18c,ESSENTIAL germinated spores at 25-32 SPBC530.01,VIABLE WT cells at 25-32 SPBC530.02,VIABLE WT cells at 25-32 SPBC530.03c,VIABLE WT cells at 25-32 SPBC530.04,VIABLE WT cells at 25-32 SPBC530.05,VIABLE WT cells at 25-32 SPBC530.06c,VIABLE WT cells at 25-32 SPBC530.07c,VIABLE WT cells at 25-32 SPBC530.08,VIABLE WT cells at 25-32 SPBC530.09c,VIABLE WT cells at 25-32 SPBC530.10c,VIABLE WT cells at 25-32 SPBC530.11c,VIABLE WT cells at 25-32 SPBC530.12c,ESSENTIAL spores- some germinated spores at 25-32 SPBC530.13,VIABLE WT cells at 25-32 SPBC530.14c,VIABLE WT cells at 25-32 SPBC530.15c,VIABLE WT cells at 25-32 SPBC543.02c,VIABLE WT cells at 25-32 SPBC543.03c,VIABLE WT cells at 25-32 SPBC543.04,ESSENTIAL spores- germinated spores at 25-32 SPBC543.05c,VIABLE WT cells at 25-32 SPBC543.06c,ESSENTIAL germinated spores misshapen at 25-32- germinated spores occasionally long at 25 SPBC543.07,VIABLE stubby cells- occasionally long at 25-32- more septated branched cells at 36 SPBC543.08,VIABLE small colonies WT cells at 25- ESSENTIAL spores- germinated spores at 32 SPBC543.09,VIABLE WT cells at 25-32 SPBC543.10,VIABLE WT cells at 25-32 SPBC557.02c,VIABLE WT cells at 25-32 SPBC557.03c,ESSENTIAL spores at 25-32 SPBC557.04,VIABLE WT cells at 25-32 SPBC557.05,VIABLE WT cells at 25-32- occasionally slightly curved SPBC56F2.01,VIABLE WT cells at 25-32 SPBC56F2.02,VIABLE WT cells at 25-32 SPBC56F2.03,VIABLE WT cells at 25-32 SPBC56F2.04,VIABLE WT cells at 25-32 SPBC56F2.05c,VIABLE WT cells at 25-32 SPBC56F2.06,VIABLE WT cells at 25-32 SPBC56F2.07c,ESSENTIAL spores- some germinated spores at 25-32 SPBC56F2.08c,VIABLE WT cells at 25-32 SPBC56F2.09c,VIABLE WT cells at 25-32 SPBC56F2.10c,VIABLE slightly misshapen slightly stubby cells at 25-32 SPBC56F2.11,VIABLE small colonies WT cells at 25-32 SPBC56F2.12,VIABLE WT cells at 25-32 SPBC56F2.14,VIABLE WT cells at 25-32 SPBC577.02,VIABLE small colonies WT cells at 25-32 SPBC577.03c,VIABLE WT cells at 25-32 SPBC577.04,VIABLE WT cells at 25-32 SPBC577.05c,VIABLE WT cells at 25-32 SPBC577.06c,VIABLE WT cells at 25-32 SPBC577.07,ESSENTIAL microcolonies misshapen cells- often long at 25-32 SPBC577.08c,VIABLE WT cells at 25-32 SPBC577.09,ESSENTIAL spores- germinated spores at 25-32 SPBC577.10,ESSENTIAL spores- germinated spores long at 25-32 SPBC577.11,VIABLE WT cells at 25-32 SPBC577.12,VIABLE WT cells at 25-32 SPBC577.13,VIABLE WT cells at 25-32 SPBC577.14c,VIABLE WT cells at 25-32 SPBC577.15c,VIABLE small colonies slightly misshapen- vacuolated cells- some long at 25-32 SPBC582.03,ESSENTIAL germinated spores long- swollen centre at 25-32 SPBC582.04c,VIABLE WT cells at 25-32 SPBC582.05c,VIABLE WT cells with some long cells at 25-32 SPBC582.06c,VIABLE WT cells at 25-32 SPBC582.07c,ESSENTIAL spores- germinated spores at 25-32 SPBC582.08,VIABLE WT cells at 25-32 SPBC582.09,VIABLE WT cells at 25-32 SPBC582.10c,VIABLE WT cells at 25-32 SPBC609.01,ESSENTIAL spores- germinated spores- microcolonies skittles cells at 25-32 SPBC609.02,VIABLE WT cells at 25-32 SPBC609.03,VIABLE WT cells at 25-32 SPBC609.04,VIABLE WT cells at 25-32 SPBC609.05,VIABLE slightly misshapen cells at 25-32 SPBC646.02,VIABLE slightly misshapen cells- occasionally curved- occasionally long 25-32 SPBC646.03,ESSENTIAL microcolonies skittle cells at 32- small colonies skittle cells at 25 SPBC646.04,ESSENTIAL microcolonies rounded septated cells at 25-32 SPBC646.05c,ESSENTIAL spores- germinated spores slightly misshapen may divide once at 25-32 SPBC646.06c,VIABLE WT cells at 25-32 SPBC646.07c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC646.08c,VIABLE WT cells at 25-32 SPBC646.09c,VIABLE WT cells at 25-32 SPBC646.10c,ESSENTIAL spores at 25-32 SPBC646.11,ESSENTIAL spores at 25-32 SPBC646.12c,VIABLE WT cells at 25-32 SPBC646.13,VIABLE WT cells at 25-32 SPBC646.14c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC646.15c,VIABLE WT cells at 25-32 SPBC646.16,ESSENTIAL spores- germinated spores- often misshapen at 25-32 SPBC646.17c,VIABLE WT cells at 25-32 SPBC649.02,VIABLE WT cells at 25-32 SPBC649.03,VIABLE WT cells at 25-32 SPBC649.04,VIABLE WT cells at 25-32 SPBC649.05,ESSENTIAL spores- germinated spores at 25-32 SPBC651.01c,ESSENTIAL spores at 25-32 SPBC651.02,VIABLE WT cells at 25-32 SPBC651.03c,VIABLE WT cells at 25-32 SPBC651.04,VIABLE WT cells at 25-32 SPBC651.05c,VIABLE WT cells at 25-32 SPBC651.06,VIABLE WT cells at 25-32 SPBC651.07,VIABLE WT cells at 25-32 SPBC651.08c,ESSENTIAL spores at 25-32 SPBC651.09c,VIABLE small colonies long cells at 25-32 SPBC651.10,VIABLE small colonies long cells at 25-32 many longer cells possibly diploidising SPBC651.11c,VIABLE WT cells at 25-32 SPBC651.12c,VIABLE WT cells at 25-32 SPBC660.05,VIABLE WT cells at 25-32 SPBC660.06,VIABLE WT cells at 25-32 SPBC660.07,VIABLE WT cells at 25-32 SPBC660.08,VIABLE WT cells at 25-32 SPBC660.09,VIABLE WT cells at 25-32 SPBC660.10,VIABLE WT cells at 25-32 SPBC660.11,VIABLE small colonies slightly misshapen- vacuolated cells at 25-32 SPBC660.12c,VIABLE WT cells at 25-32 SPBC660.13c,ESSENTIAL germinated spores slightly misshapen and divide once or twice at 25-32 SPBC660.14,VIABLE WT cells at 25-32 SPBC660.15,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC660.16,ESSENTIAL spores- germinated spores at 25-32 SPBC660.17c,VIABLE WT cells at 25-32 SPBC685.02,VIABLE WT cells at 25-32 SPBC685.03,VIABLE WT cells at 25-32 SPBC685.04c,VIABLE WT cells at 25-32 SPBC685.05,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBC685.06,VIABLE WT cells at 25-32 SPBC685.07c,VIABLE slightly misshapen vacuolated cells at 32 SPBC685.09,ESSENTIAL germinated spores long- microcolonies long cells at 25-32 SPBC691.01,VIABLE WT cells at 25-32 SPBC691.02c,ESSENTIAL spores- some germinated spores at 25-32 SPBC691.03c,VIABLE WT cells at 25-32 SPBC691.04,VIABLE WT cells at 32- microcolonies skittle cells at 25 SPBC691.05c,VIABLE WT cells at 25-32 SPBC6B1.02,VIABLE WT cells at 25-32 SPBC6B1.03c,VIABLE WT cells at 25-32 SPBC6B1.04,VIABLE slightly misshapen cells at 25-32 SPBC6B1.05c,VIABLE WT cells at 25-32 SPBC6B1.06c,VIABLE WT cells at 25-32 SPBC6B1.07,ESSENTIAL germinated spores long at 25-32 SPBC6B1.08c,VIABLE WT cells at 25-32 SPBC6B1.09c,VIABLE WT cells at 25-32 SPBC6B1.10,VIABLE WT cells at 25-32 SPBC713.02c,VIABLE WT cells at 25-32 SPBC713.03,VIABLE WT cells at 25-32 SPBC713.04c,ESSENTIAL spores- some germinated spores at 25-32 SPBC713.05,VIABLE WT cells at 25-32 SPBC713.06,VIABLE WT cells at 25-32 SPBC713.07c,VIABLE WT cells at 25-32 SPBC713.08, VIABLE slightly skittle cells 25-32 SPBC713.09,VIABLE WT cells at 25-32 SPBC713.10,ESSENTIAL spores- germinated spores at 25-32 SPBC713.11c,VIABLE WT cells at 25-32 SPBC713.12,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC725.01,VIABLE WT cells at 25-32 SPBC725.02,VIABLE small colonies stubby cells at 25-32 SPBC725.03,VIABLE WT cells at 25-32 SPBC725.04,VIABLE WT cells at 25-32 SPBC725.05c,VIABLE WT cells at 25-32 SPBC725.06c,VIABLE WT cells at 25-32 SPBC725.07,VIABLE WT cells at 25-32 SPBC725.08,ESSENTIAL microcolonies long slight skittle cells at 25-32 SPBC725.09c,VIABLE small colonies slightly misshapen cells at 25 cells long misshapen 32 and lyses SPBC725.10,VIABLE WT cells at 25-32 SPBC725.11c,VIABLE WT cells at 25-32 SPBC725.13c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC725.14,VIABLE WT cells at 25-32 SPBC725.15,VIABLE WT cells at 25-32 SPBC725.16,ESSENTIAL microcolonies long cells at 25- small colonies long cells at 32 SPBC725.17c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC776.01,VIABLE WT cells at 25-32 SPBC776.02c,VIABLE WT cells at 25-32 SPBC776.03,VIABLE WT cells at 25-32 SPBC776.04,VIABLE slightly misshapen cells at 25-32 SPBC776.05,VIABLE WT cells at 25-32 SPBC776.06c,VIABLE WT cells at 25-32 SPBC776.07,ESSENTIAL microcolonies skittle cells at 25-32 SPBC776.08c,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC776.09,VIABLE WT cells at 25-32 SPBC776.10c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPBC776.11,VIABLE WT cells at 25-32 SPBC776.12c,ESSENTIAL germinated spores long at 25-32 SPBC776.13,ESSENTIAL spores- germinated spores- occasionally long at 25-32- SPBC776.14,VIABLE WT cells at 25-32 SPBC776.15c,VIABLE slightly misshapen cells at 25-32 SPBC776.16,VIABLE WT cells at 25-32 SPBC776.17,VIABLE small colonies WT cells at 25-32 SPBC776.18c,ESSENTIAL microcolonies branched septated cells- slightly long at 25-32 SPBC800.02,VIABLE WT cells at 25-32 SPBC800.03,VIABLE WT cells at 25-32 SPBC800.04c,VIABLE small colonies WT cells at 25-32 SPBC800.05c,VIABLE WT cells at 25-32- cells more curved at 36 SPBC800.06,ESSENTIAL germinated spores slightly misshapen septated- often divide once at 25-32 SPBC800.07c,VIABLE WT cells at 25-32 SPBC800.08,VIABLE WT cells at 25-32 SPBC800.09,VIABLE WT cells at 25-32 SPBC800.10c,VIABLE WT cells at 25-32 SPBC800.11,VIABLE WT cells at 25-32 SPBC800.12c,VIABLE WT cells at 25-32 SPBC800.13,ESSENTIAL small colonies misshapen cells at 25-32 SPBC83.01,VIABLE WT cells at 25-32 SPBC83.02c,VIABLE small colonies WT cells at 25-32- some larger colonies after 2 days at 32 possibly suppressors SPBC83.03c,VIABLE slightly misshapen cells at 25-32 SPBC83.04,VIABLE WT cells at 25-32 SPBC83.05,VIABLE WT cells at 25-32 SPBC83.06c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC83.07,ESSENTIAL microcolonies branched septated cells- slightly long at 25-32 SPBC83.08,ESSENTIAL spores at 25-32 SPBC83.09c,VIABLE WT cells at 25-32 SPBC83.10,VIABLE WT cells at 25-32 SPBC83.11,VIABLE WT cells at 25-32 SPBC83.12,VIABLE WT cells at 25-32 SPBC83.13,VIABLE WT cells at 25-32 SPBC83.14c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC83.15,ESSENTIAL microcolonies long cells- often misshapen at 25-32 SPBC83.16c,VIABLE WT cells at 25-32 SPBC83.17,VIABLE WT cells at 25-32 SPBC83.18c,VIABLE slightly misshapen cells at 25-32 SPBC83.19c,VIABLE WT cells at 25-32 SPBC839.02,VIABLE WT cells at 25-32 SPBC839.03c,VIABLE WT cells at 25-32 SPBC839.04,VIABLE WT cells at 25-32 SPBC839.05c,VIABLE WT cells at 25-32 SPBC839.06,VIABLE WT cells at 25-32 SPBC839.07,VIABLE WT cells at 25-32 SPBC839.08c,ESSENTIAL microcolonies rounded cells- lysis at 25-32 SPBC839.09c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC839.10,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBC839.11c,VIABLE WT cells at 25-32 SPBC839.12,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBC839.13c,VIABLE small colonies slightly misshapen cells at 25-32 SPBC839.14c,VIABLE WT cells at 25-32 SPBC839.15c,VIABLE WT cells at 32 SPBC839.16,VIABLE WT cells at 25-32 SPBC839.17c,VIABLE WT cells at 25-32 SPBC887.01,VIABLE WT cells at 25-32 SPBC887.02,VIABLE WT cells at 25-32 SPBC887.03c,ESSENTIAL spores- some germinated spores at 25-32 SPBC887.04c,VIABLE WT cells at 32 SPBC887.05c,VIABLE small colonies long cells at 25- cells barely grow at 32 SPBC887.06c,VIABLE WT cells at 25-32 SPBC887.07,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC887.08,VIABLE WT cells at 25-32 SPBC887.09c,ESSENTIAL microcolonies rounded cells at 25-32 SPBC887.10,VIABLE long cells at 25-32 SPBC887.11,VIABLE WT cells at 25-32 SPBC887.12,ESSENTIAL microcolonies long wide cells at 25-32 SPBC887.13c,ESSENTIAL microcolonies misshapen cells at 25-32 SPBC887.14c,ESSENTIAL germinated spores long- microcolonies with some long cells at 25-32 SPBC887.15c,VIABLE WT cells at 25-32 SPBC887.17,VIABLE WT cells at 25-32 SPBC887.18c,VIABLE very small colonies branched septated slightly long cells at 32 SPBC887.19,ESSENTIAL spores- germinated spores- occasionally microcolonies misshapen cells at 25-32 SPBC8D2.01,VIABLE WT cells at 25-32 SPBC8D2.02c,VIABLE WT cells at 25-32 SPBC8D2.03c,VIABLE WT cells at 25-32 SPBC8D2.04,VIABLE WT cells at 25-32 SPBC8D2.05c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBC8D2.06,ESSENTIAL spores- germinated spores at 25-32 SPBC8D2.07c,ESSENTIAL microcolonies long cells at 25-32 SPBC8D2.09c,ESSENTIAL germinated spores long at 32- microcolonies long cells at 25 SPBC8D2.10c,VIABLE WT cells at 25-32 SPBC8D2.11,VIABLE WT cells at 25-32 SPBC8D2.12c,VIABLE WT cells at 25-32 SPBC8D2.13,ESSENTIAL microcolonies long cells at 25-32 SPBC8D2.14c,ESSENTIAL spores- germinated spores slightly misshapen may divide once at 25-32 SPBC8D2.15,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBC8D2.16c,VIABLE WT cells at 25-32 SPBC8D2.17,VIABLE slightly misshapen cells at 25-32 SPBC8D2.18c,VIABLE WT cells at 25-32 SPBC8D2.19,VIABLE WT cells at 25-32 SPBC8D2.20c,VIABLE WT cells at 25-32 SPBC8E4.01c,VIABLE WT cells at 25-32 SPBC8E4.02c,VIABLE WT cells at 25-32 SPBC8E4.03,VIABLE WT cells at 25-32 SPBC8E4.04,VIABLE WT cells at 25-32 SPBC8E4.05c,VIABLE WT cells at 25-32 SPBC902.02c,VIABLE WT cells at 25-32 SPBC902.03,VIABLE WT cells at 25-32 SPBC902.04,VIABLE WT cells at 25-32 SPBC902.05c,VIABLE WT cells at 25-32 SPBC902.06,VIABLE curved cells at 25-32 SPBC947.01,VIABLE WT cells at 25-32 SPBC947.02,VIABLE small colonies slightly misshapen cells at 25-32 SPBC947.03c,VIABLE WT cells at 25-32 SPBC947.04,VIABLE slightly misshapen cells at 25-32 SPBC947.05c,VIABLE WT cells at 25-32 SPBC947.06c,VIABLE WT cells at 25-32 SPBC947.07,ESSENTIAL spores- germinated spores- occasionally microcolonies small cells at 25-32 SPBC947.08c,VIABLE long cells at 25-32 SPBC947.09,VIABLE WT cells at 25-32 SPBC947.10,VIABLE WT cells at 25-32 SPBC947.11c,VIABLE WT cells at 25-32 SPBC947.12,ESSENTIAL spores- germinated spores long- microcolonies long cells at 25-32 SPBC947.13,VIABLE WT cells at 25-32 SPBC947.14c,VIABLE WT cells at 25-32 SPBC947.15c,VIABLE skittle cells at 25-32 SPBC9B6.03,VIABLE WT cells at 25-32 SPBC9B6.04c,ESSENTIAL microcolonies skittle cells at 25-32 SPBC9B6.05c,ESSENTIAL microcolonies long cells at 25-32 SPBC9B6.06,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPBC9B6.07,VIABLE WT cells at 25-32- small colonies curved misshapen cells- often septated at 36 SPBC9B6.08,ESSENTIAL germinated spores misshapen at 25-32 SPBC9B6.09c,VIABLE WT cells at 25-32 SPBC9B6.10,ESSENTIAL misshapen germinated spores- microcolonies misshapen cells at 25-32 SPBC9B6.11c,VIABLE WT cells at 25-32 SPBCPT2R1.01c,VIABLE WT cells at 25-32 SPBCPT2R1.02,VIABLE WT cells at 25-32 SPBCPT2R1.03,VIABLE WT cells at 25-32 SPBCPT2R1.04c,VIABLE WT cells at 25-32 SPBCPT2R1.08c,VIABLE WT cells at 32 SPBP16F5.02,ESSENTIAL microcolonies long septated cells- often curved and occasionally branched at 25-32 SPBP16F5.03c,VIABLE WT cells at 25-32 SPBP16F5.04,VIABLE WT cells at 25-32 SPBP16F5.05c,VIABLE WT cells at 25-32 SPBP16F5.06,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPBP16F5.07,VIABLE small colonies long misshapen cells at 32- cells slightly misshapen at 25 SPBP16F5.08c,VIABLE WT cells at 25-32 SPBP18G5.02,ESSENTIAL microcolonies skittle cells at 25-32 SPBP18G5.03,VIABLE WT cells at 25-32 SPBP19A11.01,ESSENTIAL microcolonies slightly misshapen cells- some germinated spores long at 25-32 SPBP19A11.02c,VIABLE WT cells at 25-32 SPBP19A11.03c,ESSENTIAL spores- germinated spores at 25-32 SPBP19A11.04c,ESSENTIAL microcolonies rounded cells at 25-32 SPBP19A11.06,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBP22H7.02c,ESSENTIAL spores- germinated spores at 32- germinated spores often long at 25 SPBP22H7.03,ESSENTIAL very swollen branched germinated spores- initially germinated spores long at 25-32 SPBP22H7.04,VIABLE WT cells at 25-32 SPBP22H7.05c,VIABLE WT cells at 25-32 SPBP22H7.06,VIABLE WT cells at 25-32 SPBP22H7.07,ESSENTIAL germinated spores long at 25-32 SPBP22H7.08,VIABLE WT cells at 32 SPBP22H7.09c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBP23A10.02,VIABLE WT cells at 32 SPBP23A10.03c,VIABLE WT cells at 25-32 SPBP23A10.04,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBP23A10.05,VIABLE WT cells at 25-32 SPBP23A10.06,ESSENTIAL microcolonies rounded cells at 25-32 SPBP23A10.07,ESSENTIAL spores- germinated spores at 25-32 SPBP23A10.08,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPBP23A10.09,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBP23A10.10,VIABLE WT cells at 25-32 SPBP23A10.12,VIABLE WT cells at 25-32 SPBP23A10.13,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25- germinated spores long at 32 SPBP23A10.14c,VIABLE WT cells at 25-32 SPBP23A10.15c,ESSENTIAL spores at 25-32 SPBP23A10.16,VIABLE WT cells at 25-32 SPBP26C9.02c,VIABLE WT cells at 25-32 SPBP26C9.03c,VIABLE WT cells at 25-32 SPBP35G2.02,VIABLE WT cells- some dead cells at 25-32 SPBP35G2.03c,VIABLE WT cells at 25-32 SPBP35G2.04c,VIABLE WT cells at 25-32 SPBP35G2.05c,VIABLE WT cells at 25-32 SPBP35G2.06c,VIABLE WT cells at 25-32 SPBP35G2.07,VIABLE WT cells at 25-32 SPBP35G2.08c,VIABLE WT cells at 25-32 SPBP35G2.09,ESSENTIAL microcolonies long cells at 25-32 SPBP35G2.10,VIABLE WT cells at 25-32 SPBP35G2.11c,VIABLE WT cells at 25-32 SPBP35G2.12,VIABLE WT cells at 25-32 SPBP35G2.13c,VIABLE curved cells at 25-32 SPBP35G2.14,VIABLE WT cells at 25-32 SPBP4G3.02,VIABLE WT cells at 25-32 SPBP4G3.03,VIABLE WT cells at 25-32 SPBP4H10.03,VIABLE slightly misshapen cells at 25- microcolonies skittle cells at 32 SPBP4H10.04,VIABLE slightly misshapen multi septated cells at 25-32-cells grow better at 32 than 25 SPBP4H10.05c,VIABLE WT cells at 25-32 SPBP4H10.06c,ESSENTIAL spores- germinated spores slightly misshapen occasionally long- some division at 25-32 SPBP4H10.07,VIABLE WT cells at 25-32 SPBP4H10.08,VIABLE slightly misshapen cells at 25-32 SPBP4H10.09,VIABLE WT cells at 25-32 SPBP4H10.10,VIABLE WT cells at 25-32 SPBP4H10.11c,VIABLE small colonies stubby cells at 25-32 SPBP4H10.12,VIABLE WT cells at 25-32 SPBP4H10.13,VIABLE WT cells at 25-32 SPBP4H10.14c,VIABLE WT cells at 25-32 SPBP4H10.15,ESSENTIAL microcolonies skittle cells at 25-32 SPBP4H10.16c,VIABLE WT cells at 25-32 SPBP4H10.17c,VIABLE WT cells at 25-32 SPBP4H10.18c,VIABLE WT cells at 25-32 SPBP4H10.19c,VIABLE WT cells at 32 SPBP4H10.20,VIABLE small colonies slightly misshapen cells at 25-32 SPBP4H10.21c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBP8B7.01c,ESSENTIAL microcolonies small cells at 25-32 SPBP8B7.02,VIABLE WT cells at 25-32 SPBP8B7.03c,ESSENTIAL spores- germinated spores misshapen at 25-32 SPBP8B7.04,VIABLE WT cells at 25-32 SPBP8B7.05c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPBP8B7.06,VIABLE WT cells at 25-32 SPBP8B7.07c,VIABLE WT cells at 25-32 SPBP8B7.08c,VIABLE WT cells at 25-32 SPBP8B7.09c,VIABLE WT cells at 25-32 SPBP8B7.10c,VIABLE WT cells at 25-32 SPBP8B7.11,VIABLE WT cells at 25-32 SPBP8B7.12c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPBP8B7.13,VIABLE slightly misshapen vacuolated cells at 25-32 SPBP8B7.14c,ESSENTIAL germinated spores long at 25-32 SPBP8B7.15c,ESSENTIAL spores- germinated spores at 25-32 SPBP8B7.16c,ESSENTIAL germinated spores long misshapen at 25-32 SPBP8B7.17c,ESSENTIAL microcolonies slightly long cells-many dead cells at 25-32 SPBP8B7.18c,VIABLE WT cells at 25-32 SPBP8B7.19,ESSENTIAL spores- some germinated spores at 25-32 SPBP8B7.20c,ESSENTIAL germinated spores at 25-32 SPBP8B7.21,VIABLE WT cells at 25-32 SPBP8B7.22,VIABLE WT cells at 25-32 SPBP8B7.23,VIABLE WT cells at 25-32 SPBP8B7.24c,VIABLE WT cells at 25-32 SPBP8B7.25,VIABLE WT cells at 25-32 SPBP8B7.26,VIABLE WT cells at 25-32 SPBP8B7.27,VIABLE WT cells at 25-32 SPBP8B7.28c,VIABLE slightly misshapen cells- some curved at 25-32 SPBP8B7.29,ESSENTIAL spores- germinated spores at 25-32 SPBP8B7.30c,VIABLE WT cells at 25-32 SPBP8B7.31,VIABLE WT cells at 25-32 SPBPB10D8.01,VIABLE WT cells at 25-32 SPBPB10D8.02c,VIABLE WT cells at 25-32 SPBPB10D8.04c,VIABLE WT cells at 25-32 SPBPB10D8.05c,VIABLE WT cells at 25-32 SPBPB10D8.06c,VIABLE WT cells at 25-32 SPBPB10D8.07c,VIABLE WT cells at 25-32 SPBPB21E7.01c,VIABLE WT cells at 25-32 SPBPB21E7.04c,VIABLE WT cells at 25-32 SPBPB21E7.05,VIABLE WT cells at 25-32 SPBPB21E7.09,VIABLE WT cells at 25-32 SPBPB2B2.01,VIABLE WT cells at 25-32 SPBPB2B2.02,VIABLE WT cells at 25-32 SPBPB2B2.05,VIABLE WT cells at 25-32 SPBPB2B2.06c,VIABLE WT cells at 25-32 SPBPB2B2.07c,VIABLE WT cells at 25-32 SPBPB2B2.08,VIABLE WT cells at 25-32 SPBPB2B2.09c,VIABLE WT cells at 25-32 SPBPB2B2.10c,VIABLE WT cells at 25-32 SPBPB2B2.11,VIABLE WT cells at 25-32 SPBPB2B2.12c,VIABLE WT cells at 25-32 SPBPB2B2.13,VIABLE WT cells at 25-32 SPBPB2B2.14c,VIABLE WT cells at 25-32 SPBPB2B2.15,VIABLE WT cells at 25-32- small colonies slightly misshapen cells at 36 SPBPB2B2.16c,VIABLE WT cells with some long cells at 25-32 SPBPB2B2.18,VIABLE WT cells at 25-32 SPBPB2B2.19c,VIABLE WT cells at 25-32 SPBPB7E8.01,VIABLE WT cells at 32 SPBPB7E8.02,VIABLE WT cells at 25-32 SPBPB8B6.04c,VIABLE WT cells at 25-32 SPBPB8B6.05c,VIABLE WT cells at 25-32 SPBPJ4664.01,VIABLE WT cells at 25-32 SPBPJ4664.02,VIABLE WT cells at 25-32 SPBPJ4664.03,VIABLE WT cells at 25-32 SPBPJ4664.04,ESSENTIAL spores- germinated spores at 25-32 SPBPJ4664.05,VIABLE WT cells at 25-32 SPBPJ4664.06,VIABLE WT cells at 25-32 SPCC1020.01c,VIABLE WT cells at 25-32 SPCC1020.02,ESSENTIAL spores- germinated spores long at 25-32 SPCC1020.03,VIABLE WT cells at 25-32 SPCC1020.04c,ESSENTIAL spores- germinated spores at 25-32 SPCC1020.05,VIABLE WT cells at 25-32 SPCC1020.06c,VIABLE WT cells at 25-32 SPCC1020.07,VIABLE WT cells at 25-32 SPCC1020.08,VIABLE WT cells at 25-32 SPCC1020.09,VIABLE WT cells at 25-32 SPCC1020.10,VIABLE WT cells at 25-32 SPCC1020.11c,VIABLE misshapen cells at 25-32 SPCC1020.12c,VIABLE WT cells at 25-32 SPCC1020.13c,VIABLE WT cells at 25-32 SPCC10H11.01,ESSENTIAL germinated spores- occasionally long at 32 not so long at 25 SPCC10H11.02,ESSENTIAL germinated spores long at 32- more division at 25 SPCC1183.01,ESSENTIAL microcolonies misshapen cells at 25 longer cells at 32 SPCC1183.02,VIABLE WT cells at 25-32 SPCC1183.03c,ESSENTIAL germinated spores misshapen at 25-32 SPCC1183.04c,VIABLE WT cells at 25-32 SPCC1183.05c,VIABLE WT cells at 25-32 SPCC1183.06,VIABLE WT cells at 25-32 SPCC1183.07,ESSENTIAL spores at 25-32 SPCC1183.08c,ESSENTIAL spores- germinated spores at 25-32 SPCC1183.09c,VIABLE WT cells at 25-32 SPCC1183.10,VIABLE WT cells at 25-32 SPCC1183.11,VIABLE WT cells at 25-32 SPCC11E10.01,VIABLE WT cells at 25-32 SPCC11E10.02c,ESSENTIAL spores- some germinated spores at 25-32 SPCC11E10.03,VIABLE WT cells at 25-32 SPCC11E10.04,VIABLE WT cells at 25-32 SPCC11E10.05c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPCC11E10.06c,VIABLE small colonies long cells at 25-32- cells curved aberrantly septated with misplaced septum at 36 SPCC11E10.07c,VIABLE WT cells at 25-32 SPCC11E10.08,VIABLE WT cells at 25-32 SPCC11E10.09c,VIABLE WT cells at 25-32 SPCC1223.01,VIABLE WT cells at 25-32 SPCC1223.02,VIABLE WT cells at 25-32 SPCC1223.03c,VIABLE WT cells at 25-32 SPCC1223.04c,VIABLE WT cells at 25-32 SPCC1223.05c,VIABLE small colonies slightly misshapen cells at 25-32 SPCC1223.06,VIABLE slightly curved cells at 25-32- occasionally cells are T-shaped SPCC1223.07c,VIABLE WT cells at 25-32 SPCC1223.08c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC1223.09,VIABLE WT cells at 25-32 SPCC1223.10c,VIABLE WT cells at 25-32 SPCC1223.11,VIABLE WT cells at 25-32 SPCC1223.12c,VIABLE WT cells at 25-32 SPCC1223.13,VIABLE WT cells at 25-32 SPCC1223.14,ESSENTIAL germinated spores at 25-32 SPCC1223.15c,VIABLE small colonies WT cells at 25-32 SPCC1235.01,VIABLE WT cells at 25-32 SPCC1235.02,VIABLE WT cells at 25-32 SPCC1235.03,VIABLE WT cells at 25-32 SPCC1235.04c,VIABLE WT cells at 25-32 SPCC1235.05c,VIABLE WT cells at 25-32 SPCC1235.06,VIABLE WT cells at 25-32 SPCC1235.07,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC1235.08c,VIABLE WT cells at 25-32 SPCC1235.09,VIABLE WT cells at 25-32 SPCC1235.10c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPCC1235.11,VIABLE small colonies WT cells at 25-32 SPCC1235.12c,VIABLE WT cells at 25-32 SPCC1235.13,VIABLE WT cells at 25-32 SPCC1235.14,VIABLE WT cells at 25-32 SPCC1235.15,VIABLE WT cells at 25-32 SPCC1259.01c,VIABLE small colonies slightly misshapen- vacuolated cells at 25-32 SPCC1259.02c,VIABLE slightly misshapen cells at 25-32 SPCC1259.03,VIABLE WT cells at 32 SPCC1259.04,VIABLE WT cells at 32 SPCC1259.05c,VIABLE WT cells at 25-32 SPCC1259.06,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC1259.07, ESSENTIAL microcolonies long misshapen cells at 25-32- may be giving rise to suppressors SPCC1259.08,VIABLE WT cells at 25-32 SPCC1259.09c,VIABLE WT cells at 25-32 SPCC1259.10,VIABLE WT cells at 25-32 SPCC1259.11c,VIABLE WT cells at 25-32 SPCC1259.12c,VIABLE WT cells at 25-32 SPCC1259.13,VIABLE WT cells at 25-32 SPCC1259.14c,VIABLE WT cells at 25-32 SPCC1259.15c,ESSENTIAL germinated spores at 25-32 SPCC126.01c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPCC126.02c,VIABLE WT cells at 25-32 SPCC126.03,VIABLE WT cells at 25-32 SPCC126.04c,VIABLE slightly misshapen small cells at 25-32 SPCC126.05c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC126.06,VIABLE WT cells at 25-32 SPCC126.07c,VIABLE WT cells at 25-32 SPCC126.08c,VIABLE WT cells at 25-32 SPCC126.09,VIABLE WT cells at 25-32 SPCC126.10,VIABLE WT cells at 25-32 SPCC126.11c,VIABLE WT cells at 25-32 SPCC126.12,VIABLE WT cells at 25-32 SPCC126.13c,VIABLE WT cells at 25-32 SPCC126.14,ESSENTIAL microcolonies long misshapen cells at 25-32 SPCC126.15c,VIABLE WT cells at 25-32 SPCC1281.01,ESSENTIAL spores- germinated spores at 25-32 SPCC1281.02c,ESSENTIAL germinated spores long curved- often divide at 25-32 SPCC1281.03c,VIABLE WT cells at 25-32 SPCC1281.04,VIABLE WT cells at 25-32 SPCC1281.05,ESSENTIAL microcolonies long curved cells at 25-32 SPCC1281.06c,ESSENTIAL spores at 25-32 SPCC1281.07c,VIABLE WT cells at 25-32 SPCC1281.08,VIABLE WT cells at 25-32 SPCC132.01c,VIABLE WT cells at 25-32 SPCC132.02,VIABLE WT cells at 25-32 SPCC132.03,VIABLE WT cells at 25-32 SPCC132.04c,VIABLE WT cells at 25-32 SPCC1322.01,VIABLE WT cells at 25-32 SPCC1322.02,VIABLE WT cells at 25-32 SPCC1322.03,VIABLE WT cells at 25-32 SPCC1322.04,ESSENTIAL microcolonies long cells at 25-32 SPCC1322.05c,VIABLE WT cells at 25-32 SPCC1322.06,VIABLE WT cells at 25-32 SPCC1322.07c,VIABLE WT cells at 25-32 SPCC1322.08,VIABLE WT cells at 25-32 SPCC1322.09,VIABLE WT cells at 32 SPCC1322.10,VIABLE WT cells at 25-32 SPCC1322.11,ESSENTIAL microcolonies slightly long cells at 25-32 SPCC1322.12c,VIABLE WT cells at 25-32 SPCC1322.14c,VIABLE WT cells at 25-32 SPCC1322.15,VIABLE WT cells at 25-32 SPCC1322.16,VIABLE WT cells at 25-32 SPCC1393.02c,VIABLE WT cells at 25-32 SPCC1393.03,VIABLE WT cells at 25-32 SPCC1393.04,ESSENTIAL microcolonies slightly misshapen cells- some long cells- aberrantly septated with misplaced septum at 25-32 SPCC1393.05,VIABLE WT cells occasionally curved at 25-32 SPCC1393.06c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPCC1393.07c,VIABLE slightly misshapen cells at 25-32 SPCC1393.08,VIABLE WT cells at 25-32 SPCC1393.09c,VIABLE WT cells at 25-32 SPCC1393.10,VIABLE WT cells at 25-32 SPCC1393.11,ESSENTIAL microcolonies skittle cells at 25-32 SPCC1393.12,VIABLE WT cells at 25-32 SPCC1393.13,VIABLE WT cells at 25-32 SPCC13B11.01,VIABLE WT cells at 25-32 SPCC13B11.02c,VIABLE WT cells at 25-32 SPCC13B11.03c,VIABLE WT cells at 25-32 SPCC13B11.04c,VIABLE WT cells at 25-32 SPCC1442.01,VIABLE WT cells at 25-32 SPCC1442.02,VIABLE WT cells at 25-32 SPCC1442.03,VIABLE WT cells at 25-32 SPCC1442.04c,VIABLE WT cells at 25-32 SPCC1442.05c,VIABLE WT cells at 25-32 SPCC1442.06,ESSENTIAL spores- germinated spores slightly misshapen at 25-32 SPCC1442.07c,VIABLE WT cells at 25-32 SPCC1442.08c,VIABLE WT cells at 25-32 SPCC1442.09,ESSENTIAL spores- germinated spores at 25-32 SPCC1442.10c,ESSENTIAL germinated spores long curved at 25-32 SPCC1442.11c,VIABLE WT cells at 25-32 SPCC1442.12,ESSENTIAL microcolonies misshapen cells 32 SPCC1442.13c,VIABLE WT cells at 25-32 SPCC1442.14c,VIABLE WT cells at 25-32 SPCC1442.15c,VIABLE WT cells at 25-32 SPCC1442.16c,VIABLE WT cells at 25-32 SPCC1442.17c,VIABLE WT cells at 25-32 SPCC1450.02,VIABLE WT cells at 25-32 SPCC1450.03,VIABLE long cells at 25-32 SPCC1450.04,ESSENTIAL spores- germinated spores at 25-32 SPCC1450.05c,VIABLE WT cells at 25-32 SPCC1450.06c,VIABLE WT cells at 25-32 SPCC1450.07c,VIABLE WT cells at 25-32 SPCC1450.08c,VIABLE WT cells at 25-32 SPCC1450.09c,VIABLE WT cells at 25-32 SPCC1450.10c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPCC1450.11c,VIABLE WT cells at 25-32 SPCC1450.12,VIABLE WT cells at 25-32 SPCC1450.13c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC1450.14c,ESSENTIAL microcolonies large rounded septated cells at 25-32 SPCC1450.15,ESSENTIAL spores- some germinated spores at 25-32 SPCC1450.16c,VIABLE WT cells at 25-32 SPCC1494.01,VIABLE WT cells at 25-32 SPCC1494.02c,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC1494.03,VIABLE WT cells at 25-32 SPCC1494.04c,VIABLE small colonies WT cells at 25-32 SPCC1494.05c,VIABLE WT cells at 25-32 SPCC1494.06c,ESSENTIAL spores at 25-32 SPCC1494.07,VIABLE WT cells at 25-32 SPCC1494.08c,VIABLE WT cells at 25-32 SPCC1494.09c,VIABLE WT cells at 25-32 SPCC1494.10,VIABLE WT cells at 25-32 SPCC14G10.01,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC14G10.02,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC14G10.03c,VIABLE small colonies rounded cells at 25- spores at 32 SPCC14G10.04,VIABLE slightly misshapen cells at 32- cells misshapen at 25 SPCC1529.01,VIABLE WT cells at 25-32 SPCC162.01c,VIABLE WT cells at 25-32 SPCC162.02c,VIABLE WT cells at 25-32 SPCC162.03,VIABLE WT cells at 25-32 SPCC162.04c,VIABLE WT cells at 25-32 SPCC162.05,VIABLE slightly misshapen slightly vacuolated cells at 25-32 SPCC162.06c,VIABLE WT cells at 25-32 SPCC162.07,ESSENTIAL spores at 25-32 SPCC162.08c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC162.09c,ESSENTIAL spores- germinated spores at 25-32 SPCC162.10,VIABLE WT cells at 25-32 SPCC162.11c,VIABLE WT cells at 25-32 SPCC162.12,VIABLE WT cells at 25-32 SPCC1620.01c,ESSENTIAL microcolonies long cells at 25-32 SPCC1620.02,VIABLE WT cells at 25-32 SPCC1620.03,VIABLE WT cells at 25-32 SPCC1620.04c,VIABLE WT cells at 25-32 SPCC1620.05,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC1620.06c,ESSENTIAL spores at 25-32 SPCC1620.07c,VIABLE WT cells at 25-32 SPCC1620.08,VIABLE WT cells at 25-32 SPCC1620.09c,ESSENTIAL germinated spores- often long and curved at 25-32 SPCC1620.10,ESSENTIAL germinated spores long- often misshapen at 32- spores some germination at 25 SPCC1620.11, ESSENTIAL spores- some germinated spores long at 25-32 SPCC1620.12c,VIABLE WT cells at 25-32 SPCC1620.13,VIABLE WT cells at 25-32 SPCC1620.14c,VIABLE WT cells at 25-32 SPCC1672.01,ESSENTIAL spores- some germinated spores at 25-32 SPCC1672.02c,ESSENTIAL spores at 25-32 SPCC1672.03c,VIABLE WT cells at 25-32 SPCC1672.04c,VIABLE WT cells at 25-32 SPCC1672.05c,ESSENTIAL spores- some germinated spores at 25-32 SPCC1672.06c,VIABLE small colonies slightly misshapen cells at 25-32- cells swollen- misshapen at 36 SPCC1672.07,ESSENTIAL germinated spores at 25-32 SPCC1672.08c,ESSENTIAL spores at 25-32 SPCC1672.09,VIABLE WT cells at 25-32 SPCC1672.10,ESSENTIAL spores at 25-32 SPCC1672.11c,VIABLE WT cells at 25-32 SPCC1672.12c,VIABLE WT cells at 25-32 SPCC1682.01,VIABLE WT cells at 25-32 SPCC1682.02c,ESSENTIAL spores- germinated spores long- often very long- occasionally microcolonies with some long cells at 25-32 SPCC1682.03c,ESSENTIAL small colonies long cells at 25-32 SPCC1682.04,ESSENTIAL germinated spores misshapen- microcolonies slightly misshapen cells- some long cells some misshapen septated cells at 25-32 SPCC1682.06,VIABLE WT cells at 25-32 SPCC1682.07,ESSENTIAL microcolonies misshapen branched septated slightly long cells at 25-32 SPCC1682.08c,VIABLE WT cells at 32 SPCC1682.09c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC1682.10,ESSENTIAL spores- germinated spores at 25-32 SPCC1682.11c,VIABLE WT cells at 25-32 SPCC1682.12c,VIABLE WT cells at 25-32 SPCC1682.13,VIABLE WT cells at 25-32 SPCC1682.14,VIABLE WT cells at 25-32 SPCC1682.15,VIABLE WT cells at 32 SPCC1682.16,VIABLE WT cells at 25-32 SPCC16A11.01,VIABLE WT cells at 25-32 SPCC16A11.03c,VIABLE WT cells at 25-32- slightly misshapen at 36 SPCC16A11.04,VIABLE WT cells at 25-32 SPCC16A11.05c,ESSENTIAL germinated spores long- occasionally divide 25-32 SPCC16A11.06c,ESSENTIAL spores- germinated spores- some microcolonies slightly misshapen cells at 25-32 SPCC16A11.07,VIABLE WT cells- microcolonies skittle cells at 25-32 SPCC16A11.08,VIABLE WT cells at 25-32 SPCC16A11.09c,ESSENTIAL spores- germinated spores slightly misshapen- often septated at 25-32 SPCC16A11.10c,VIABLE WT cells at 25-32 SPCC16A11.11,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC16A11.12c,VIABLE WT cells at 25-32 SPCC16A11.13,ESSENTIAL small colonies misshapen cells- aberrantly septated with misplaced septum at 32- microcolonies long cells at 25 SPCC16A11.14,ESSENTIAL microcolonies long curved cells at 25-32 SPCC16A11.15c,VIABLE WT cells at 25-32 SPCC16A11.16c,VIABLE WT cells at 25-32 SPCC16A11.17,ESSENTIAL germinated spores long at 25-32 SPCC16C4.01,VIABLE WT cells at 25-32 SPCC16C4.02c,ESSENTIAL germinated spores long at 25-32 SPCC16C4.03,VIABLE WT cells at 25-32 SPCC16C4.04,VIABLE WT cells at 25-32 SPCC16C4.05,ESSENTIAL microcolonies small cells at 25-32 SPCC16C4.06c,VIABLE WT cells at 25-32 SPCC16C4.07,VIABLE WT cells at 25-32 SPCC16C4.08c,ESSENTIAL microcolonies misshapen branched septated slightly long cells at 25-32 SPCC16C4.09,VIABLE WT cells at 25-32 SPCC16C4.10,VIABLE WT cells at 25-32 SPCC16C4.11,VIABLE small colonies slightly misshapen cells at 25-32 SPCC16C4.12,VIABLE WT cells at 25-32 SPCC16C4.13c,VIABLE small colonies slightly misshapen cells at 25-32 SPCC16C4.14c,VIABLE WT cells at 25-32 SPCC16C4.15,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC16C4.17,VIABLE WT cells at 25-32 SPCC16C4.18c,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC16C4.19,ESSENTIAL microcolonies long cells at 25-32 SPCC16C4.20c,VIABLE slightly long cells at 25-32 SPCC1739.01,VIABLE WT cells at 25-32 SPCC1739.02c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC1739.03,VIABLE WT cells at 25-32 SPCC1739.04c,VIABLE WT cells at 25-32 SPCC1739.05,VIABLE WT cells at 25-32 SPCC1739.06c,VIABLE WT cells at 32 SPCC1739.07,VIABLE very small colonies WT cells at 25-32 SPCC1739.08c,VIABLE WT cells at 25-32 SPCC1739.09c,VIABLE WT cells at 25-32 SPCC1739.10,VIABLE WT cells at 25-32 SPCC1739.11c,ESSENTIAL germinated spores long at 25-32 SPCC1739.12,VIABLE small colonies rounded cells at 25-32 SPCC1739.13,ESSENTIAL spores at 25-32 SPCC1739.14,VIABLE long cells at 25-32 SPCC1739.15,ESSENTIAL spores at 25-32 SPCC1742.01,VIABLE WT cells at 25-32 SPCC1753.01c,ESSENTIAL germinated spores long at 25-32 SPCC1753.02c,VIABLE WT cells at 25-32 SPCC1753.03c,VIABLE WT cells- some germination long at 25-32 SPCC1753.04,ESSENTIAL germinated spores at 25-32 SPCC1753.05,VIABLE small colonies long cells at 25- cells slightly misshapen at 32 SPCC1795.01c,VIABLE WT cells at 25-32 SPCC1795.02c,VIABLE WT cells at 25-32 SPCC1795.03,VIABLE WT cells at 25-32 SPCC1795.04c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPCC1795.05c,ESSENTIAL spores- germinated spores at 25-32 SPCC1795.06,VIABLE WT cells at 25-32 SPCC1795.07,ESSENTIAL microcolonies skittle cells at 25-32 SPCC1795.08c,ESSENTIAL microcolonies stubby cells at 25- cells slightly misshapen at 32 SPCC1795.09,VIABLE WT cells at 25-32 SPCC1795.10c,VIABLE WT cells at 25-32 SPCC1795.11,ESSENTIAL spores- germinated spores slightly misshapen- occasionally divide once at 25-32 SPCC1795.12c,VIABLE WT cells at 25-32 SPCC18.01c,VIABLE WT cells at 32 SPCC18.02,VIABLE WT cells at 25-32 SPCC18.03,VIABLE WT cells at 25-32 SPCC18.04,ESSENTIAL spores- germinated spores at 25-32 SPCC18.05c,ESSENTIAL spores- germinated spores at 25-32 SPCC18.06c,VIABLE WT cells at 25-32 SPCC18.07,ESSENTIAL germinated spores slightly misshapen- often divide once at 25-32 SPCC18.08,ESSENTIAL microcolonies skittle cells at 25-32 SPCC18.09c,VIABLE WT cells at 25-32 SPCC18.10,VIABLE WT cells at 25-32 SPCC18.11c,VIABLE WT cells at 25-32 SPCC18.12c,ESSENTIAL spores- germinated spores slightly long at 25-32 SPCC18.13,VIABLE WT cells at 25-32 SPCC18.14c,ESSENTIAL spores at 25-32 SPCC18.15,VIABLE WT cells at 25-32 SPCC18.16c,ESSENTIAL spores at 25-32 SPCC18.17c,VIABLE slightly small cells at 25-32- cells slightly curved at 32- cells more curved at 36 SPCC1827.01c,ESSENTIAL germinated spores long- some division at 25-32 SPCC1827.02c,VIABLE WT cells at 32 SPCC1827.03c,VIABLE WT cells at 25-32 SPCC1827.04,VIABLE slightly misshapen cells at 25-32 SPCC1827.05c,ESSENTIAL spores at 25-32 SPCC1827.06c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPCC1827.07c,VIABLE WT cells at 25-32 SPCC1827.08c,VIABLE WT cells at 25-32 SPCC1840.01c,ESSENTIAL germinated spores long- some division at 25-32 SPCC1840.02c,VIABLE WT cells at 25-32 SPCC1840.03,VIABLE small colonies long cells at 25-32 SPCC1840.04,VIABLE WT cells at 25-32 SPCC1840.05c,VIABLE WT cells at 25-32 SPCC1840.06,VIABLE slightly misshapen cells at 25-32 SPCC1840.07c,VIABLE WT cells at 25-32 SPCC1840.08c,VIABLE WT cells at 25-32 SPCC1840.09,VIABLE WT cells at 25-32 SPCC1840.10,VIABLE WT cells at 25-32 SPCC1840.11,VIABLE WT cells at 25-32 SPCC1840.12,VIABLE WT cells at 25-32 SPCC188.02,VIABLE slightly misshapen- slightly curved cells at 25-32- cells misshapen at 36 SPCC188.03,ESSENTIAL spores- germinated spores- some long at 25-32 SPCC188.04c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC188.06c,ESSENTIAL spores at 25-32 SPCC188.07,VIABLE long slightly misshapen cells at 25-32- cells longer often curved at 36 SPCC188.08c,VIABLE WT cells at 25-32 SPCC188.09c,VIABLE WT cells at 25-32 SPCC188.11,ESSENTIAL spores- germinated spores- occasionally microcolonies misshapen cells at 25-32 SPCC188.12,VIABLE WT cells at 25-32 SPCC188.13c,VIABLE small colonies WT cells- occasionally curved at 25-32 SPCC1884.02,VIABLE WT cells at 25-32 SPCC18B5.01c,VIABLE WT cells at 25-32 SPCC18B5.03,VIABLE small colonies small cells at 25-32- very small colonies at 36 SPCC18B5.04,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC18B5.05c,VIABLE WT cells at 25-32 SPCC18B5.06,VIABLE WT cells at 25-32 SPCC18B5.07c,VIABLE WT cells at 25-32 SPCC18B5.08c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC18B5.09c,VIABLE WT cells at 25-32 SPCC18B5.10c,VIABLE WT cells at 25-32 SPCC18B5.11c,VIABLE WT cells at 25-32 SPCC1902.01,VIABLE WT cells at 25-32 SPCC1902.02,VIABLE WT cells at 25-32 SPCC1906.01,ESSENTIAL germinated spores misshapen at 25-32 SPCC1906.02c,VIABLE WT cells at 25-32 SPCC1906.03,VIABLE WT cells at 25-32 SPCC1906.04,VIABLE WT cells at 25-32 SPCC191.01,VIABLE WT cells at 25-32 SPCC191.02c,VIABLE WT cells at 25-32 SPCC191.03c,VIABLE WT cells at 25-32 SPCC191.05c,VIABLE WT cells at 32 SPCC191.06,VIABLE slightly misshapen cells at 25-32 SPCC191.07,ESSENTIAL germinated spores at 25-32 SPCC191.08,ESSENTIAL spores- germinated spores slightly misshapen- occasionally divide once at 25-32 SPCC191.09c,VIABLE WT cells at 25-32 SPCC191.10,VIABLE WT cells at 25-32 SPCC191.11,VIABLE WT cells at 32 SPCC1919.01,VIABLE WT cells at 25-32 SPCC1919.02,ESSENTIAL germinated spores misshapen- microcolonies misshapen cells at 25-32 SPCC1919.03c,VIABLE WT cells at 25-32 SPCC1919.05,VIABLE WT cells at 25-32 SPCC1919.07,VIABLE WT cells at 25-32 SPCC1919.08c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC1919.09,VIABLE WT cells at 25-32 SPCC1919.10c,VIABLE rounded misshapen- septated cells at 25-32 SPCC1919.11,VIABLE WT cells at 25-32 SPCC1919.12c,VIABLE WT cells at 25-32 SPCC1919.13c,VIABLE WT cells at 25-32 SPCC1919.14c,ESSENTIAL spores at 25-32 SPCC1919.15,VIABLE small colonies long septated cells at 25 32 SPCC23B6.01c,VIABLE small colonies rounded cells at 25-32 SPCC23B6.02c,VIABLE WT cells at 25-32 SPCC23B6.03c,VIABLE WT cells at 25-32 SPCC23B6.04c,VIABLE slightly misshapen cells at 25-32 SPCC23B6.05c,VIABLE WT cells at 25-32 SPCC24B10.02c,VIABLE WT cells at 25-32 SPCC24B10.03,VIABLE WT cells at 25-32 SPCC24B10.04,VIABLE skittle cells at 25-32 SPCC24B10.05,ESSENTIAL microcolonies skittle- rounded cells at 25-32 SPCC24B10.06,VIABLE WT cells at 25-32 SPCC24B10.07,VIABLE slightly long cells at 32- microcolonies long cells at 25 SPCC24B10.08c,VIABLE WT cells at 25-32 SPCC24B10.09,VIABLE WT cells at 25-32 SPCC24B10.10c,VIABLE WT cells at 32 SPCC24B10.11c,VIABLE very small colonies long cells at 25-32- ESSENTIAL branched cells at 36 SPCC24B10.12,VIABLE WT cells at 25-32 SPCC24B10.13,VIABLE WT cells at 25-32 SPCC24B10.14c,VIABLE WT cells at 25-32 SPCC24B10.15,VIABLE WT cells at 25-32 SPCC24B10.16c,VIABLE WT cells at 25-32 SPCC24B10.17,VIABLE WT cells at 25-32 SPCC24B10.18,VIABLE WT cells at 25-32 SPCC24B10.19c,VIABLE WT cells at 25-32 SPCC24B10.20,VIABLE WT cells at 25-32 SPCC24B10.21,VIABLE WT cells at 25-32 SPCC24B10.22,VIABLE WT cells at 25-32 SPCC285.03,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC285.04,VIABLE WT cells at 25-32 SPCC285.05,VIABLE WT cells at 25-32 SPCC285.08,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC285.09c,VIABLE WT cells at 25-32- slightly long at 36 SPCC285.10c,VIABLE WT cells at 25-32 SPCC285.11,VIABLE WT cells at 25-32 SPCC285.12,ESSENTIAL germinated spores long at 25-32 SPCC285.13c,VIABLE WT cells at 25-32 SPCC285.14,VIABLE WT cells at 25-32 SPCC285.15c,VIABLE WT cells at 25-32 SPCC285.16c,VIABLE WT cells at 25-32 SPCC285.17,VIABLE WT cells at 25-32 SPCC290.02,ESSENTIAL germinated spores slightly misshapen- often divide once at 25-32 SPCC290.03c,ESSENTIAL spores- germinated spores- some microcolonies long cells at 25-32 SPCC297.03,VIABLE long cells at 25-32 SPCC297.04c,VIABLE WT cells at 25-32 SPCC297.05,VIABLE WT cells at 25-32 SPCC297.06c,VIABLE WT cells at 32 SPCC2H8.02,VIABLE WT cells at 25-32 SPCC2H8.04,ESSENTIAL microcolonies skittle cells at 25-32 SPCC2H8.05c,VIABLE WT cells at 25-32 SPCC306.02c,VIABLE WT cells at 25-32- cells curved at 36 SPCC306.03c,ESSENTIAL spores- germinated spores- occasionally long some division at 25-32 SPCC306.04c,VIABLE long cells at 25-32 SPCC306.05c,VIABLE WT cells at 32 SPCC306.06c,ESSENTIAL spores at 25-32 SPCC306.07c,VIABLE WT cells at 25-32 SPCC306.08c,VIABLE WT cells at 25-32 SPCC306.09c,VIABLE small colonies rounded misshapen cells- sporulating on YES at 25-32 SPCC306.11,VIABLE WT cells at 25-32 SPCC31H12.02c,VIABLE WT cells at 25-32 SPCC31H12.03c,VIABLE WT cells at 32 SPCC31H12.04c,VIABLE WT cells at 25-32 SPCC31H12.05c,VIABLE WT cells at 25-32 SPCC31H12.06,VIABLE WT cells at 25-32 SPCC31H12.07,ESSENTIAL spores at 25-32 SPCC31H12.08c,VIABLE WT cells at 25-32 SPCC320.03,VIABLE WT cells at 25-32 SPCC320.04c,VIABLE WT cells with some long cells at 25-32 SPCC320.05,VIABLE WT cells at 25-32 SPCC320.06,VIABLE WT cells at 25-32 SPCC320.07c,VIABLE WT cells at 25-32 SPCC320.08,VIABLE WT cells at 25-32 SPCC320.09,ESSENTIAL microcolonies slightly misshapen cells- colonies dark in colour at 32- spores at 25 SPCC320.10,ESSENTIAL spores at 25-32 SPCC320.11c,ESSENTIAL spores- germinated spores- microcolonies slightly misshapen septated cells at 25-32 SPCC320.12,VIABLE WT cells at 25-32 SPCC320.13c,ESSENTIAL spores- germinated spores- occasionally long at 25-32- SPCC320.14,VIABLE WT cells at 25-32 SPCC330.01c,VIABLE WT cells at 25-32 SPCC330.02,VIABLE WT cells at 25-32 SPCC330.03c,VIABLE WT cells at 25-32 SPCC330.04c,ESSENTIAL germinated spores long- microcolonies WT cells at 25-32 SPCC330.06c,VIABLE WT cells at 25-32 SPCC330.07c,VIABLE WT cells at 25-32 SPCC330.08,ESSENTIAL spores- germinated spores at 25-32 SPCC330.09,ESSENTIAL germinated spores slightly misshapen septated- often divide once at 25-32 SPCC330.10,ESSENTIAL microcolonies long cells at 25-32 SPCC330.11,VIABLE slightly misshapen cells at 25-32 SPCC330.12c,VIABLE WT cells at 25-32 SPCC330.13,ESSENTIAL germinated spores slightly misshapen- often divide once at 25-32 SPCC330.14c,VIABLE WT cells at 25-32 SPCC330.19c,VIABLE WT cells at 25-32 SPCC338.02,VIABLE WT cells at 25-32 SPCC338.04,VIABLE WT cells at 25-32 SPCC338.05c,VIABLE WT cells at 25-32 SPCC338.06c,VIABLE WT cells at 25-32 SPCC338.07c,VIABLE WT cells at 25-32 SPCC338.08,VIABLE small colonies WT cells- some long cells at 25-32- possibly diploidising SPCC338.10c,VIABLE small colonies WT cells at 25-32 SPCC338.11c,VIABLE WT cells at 25-32 SPCC338.12,VIABLE WT cells at 25-32 SPCC338.13,ESSENTIAL spores at 25-32 SPCC338.14,VIABLE WT cells at 25-32 SPCC338.15,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPCC338.16,VIABLE small colonies long cells at 25-32- possibly diploidising at high frequency SPCC338.17c,ESSENTIAL germinated spores misshapen at 25-32 SPCC338.18,VIABLE WT cells at 25-32 SPCC364.01,VIABLE WT cells at 25-32 SPCC364.02c,VIABLE WT cells at 25-32 SPCC364.03,VIABLE WT cells at 25-32 SPCC364.04c,VIABLE WT cells at 25-32 SPCC364.05,VIABLE WT cells at 25-32 SPCC364.06,VIABLE WT cells at 25-32 SPCC364.07,VIABLE WT cells at 25-32 SPCC417.02,VIABLE slightly misshapen cells at 25-32- some long cells at 36 SPCC417.05c,VIABLE WT cells at 32 SPCC417.06c,VIABLE WT cells at 25-32 SPCC417.07c,VIABLE curved cells- often aberrantly septated with misplace septum at 25-32 SPCC417.08,ESSENTIAL spores at 25-32 SPCC417.09c,VIABLE WT cells at 25-32 SPCC417.10,VIABLE WT cells at 25-32 SPCC417.11c,VIABLE WT cells at 25-32 SPCC417.12,VIABLE WT cells at 25-32 SPCC4B3.02c,VIABLE WT cells at 25-32 SPCC4B3.03c,VIABLE WT cells at 25-32 SPCC4B3.04c,VIABLE WT cells at 25-32 SPCC4B3.05c,VIABLE WT cells at 25-32 SPCC4B3.06c,VIABLE WT cells at 25-32 SPCC4B3.07,VIABLE WT cells at 25-32 SPCC4B3.08,VIABLE long cells at 25-32 SPCC4B3.09c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC4B3.10c,VIABLE stubby cells at 25-32 SPCC4B3.11c,VIABLE WT cells at 25-32 SPCC4B3.12,VIABLE WT cells at 25-32 SPCC4B3.13,VIABLE WT cells at 25-32 SPCC4B3.14,ESSENTIAL microcolonies long cells at 25-32 SPCC4B3.15,VIABLE small colonies misshapen- aberrantly septated at 25-32 SPCC4B3.16,VIABLE WT cells at 25-32 SPCC4B3.17,VIABLE WT cells at 25-32 SPCC4B3.18,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC4E9.01c,VIABLE WT cells at 25-32 SPCC4E9.02,VIABLE WT cells at 25-32 SPCC4F11.02,VIABLE WT cells at 25-32 SPCC4F11.03c,VIABLE slightly misshapen cells at 25-32- cells misshapen at 36 SPCC4F11.04c,VIABLE very small colonies rounded cells at 32 SPCC4G3.02,VIABLE WT cells at 25-32 SPCC4G3.03,VIABLE WT cells at 25-32 SPCC4G3.04c,VIABLE WT cells at 25-32 SPCC4G3.05c,VIABLE slightly long cells at 32 SPCC4G3.06c,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC4G3.07c,ESSENTIAL spores- germinated spores- occasionally microcolonies misshapen cells at 25-32 SPCC4G3.08,VIABLE WT cells at 25-32 SPCC4G3.09c,VIABLE WT cells at 25-32 SPCC4G3.10c,VIABLE WT cells at 32 SPCC4G3.11,VIABLE WT cells at 25-32 SPCC4G3.12c,VIABLE WT cells at 25-32 SPCC4G3.13c,VIABLE WT cells at 25-32 SPCC4G3.14,ESSENTIAL microcolonies skittle cells at 32- larger colonies at 25 SPCC4G3.15c,VIABLE WT cells at 25-32 SPCC4G3.16,ESSENTIAL spores at 25-32 SPCC4G3.17,VIABLE WT cells at 25-32 SPCC4G3.18,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPCC4G3.19,VIABLE WT cells at 25-32 SPCC548.05c,VIABLE WT cells at 25-32 SPCC548.06c,VIABLE WT cells at 32 SPCC548.07c,VIABLE WT cells at 25-32 SPCC550.01c,VIABLE WT cells at 25-32 SPCC550.02c,ESSENTIAL germinated spores long- some division at 25-32 SPCC550.03c,VIABLE WT cells at 25-32 SPCC550.04c,ESSENTIAL germinated spores slightly misshapen- divide once at 25-32 SPCC550.05,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC550.06c,ESSENTIAL microcolonies rounded cells at 25-32 SPCC550.07,VIABLE WT cells at 25-32 SPCC550.08,VIABLE WT cells at 25-32 SPCC550.09,VIABLE WT cells at 32 SPCC550.10,VIABLE WT cells at 25-32 SPCC550.11,VIABLE WT cells at 25-32 SPCC550.12,VIABLE slightly curved cells at 25-32 SPCC550.13,ESSENTIAL germinated spores- occasionally long- some microcolonies of long cells at 25-32 SPCC550.14,VIABLE WT cells at 25-32 SPCC550.15c,VIABLE WT cells at 25-32 SPCC553.01c,VIABLE WT cells at 25-32 SPCC553.02,ESSENTIAL microcolonies stubby cells at 25-32 SPCC553.03,VIABLE WT cells at 25-32 SPCC553.04,VIABLE WT cells at 25-32 SPCC553.06,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPCC553.07c,VIABLE WT cells at 25-32 SPCC553.08c,VIABLE WT cells at 25-32 SPCC553.09c,ESSENTIAL germinated spores long at 25-32 SPCC553.11c,ESSENTIAL microcolonies misshapen septated cells at 25-32 SPCC553.12c,VIABLE WT cells at 25-32 SPCC569.01c,VIABLE WT cells at 25-32 SPCC569.02c,VIABLE WT cells at 32 SPCC569.03,VIABLE WT cells at 32 SPCC569.04,VIABLE WT cells at 25-32 SPCC569.05c,VIABLE WT cells at 25-32 SPCC569.06,VIABLE WT cells at 25-32 SPCC569.07,VIABLE WT cells at 25-32 SPCC569.08c,VIABLE WT cells at 25-32 SPCC576.01c,VIABLE WT cells at 25-32 SPCC576.02,VIABLE WT cells at 25-32 SPCC576.03c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPCC576.04,VIABLE WT cells at 25-32 SPCC576.05,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC576.06c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC576.07,ESSENTIAL spores- germinated spores at 25-32 SPCC576.08c,ESSENTIAL spores at 25-32 SPCC576.09,ESSENTIAL spores- germinated spores at 25-32 SPCC576.10c,ESSENTIAL germinated spores at 25-32 SPCC576.11,VIABLE small colonies slightly misshapen cells at 25-32 SPCC576.12c,VIABLE long slightly misshapen cells at 25-32 SPCC576.13,VIABLE WT cells at 25-32 SPCC576.14,VIABLE WT cells at 25-32 SPCC576.15c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC576.17c,VIABLE WT cells at 25-32 SPCC584.01c,VIABLE WT cells at 25-32 SPCC584.02,VIABLE WT cells at 25-32 SPCC584.03c,VIABLE WT cells at 25-32 SPCC584.04,ESSENTIAL spores at 25-32 SPCC584.05,ESSENTIAL spores- germinated spores slightly misshapen divide once at 25-32 SPCC584.11c,VIABLE small colonies rounded cells at 25-32 SPCC584.12,VIABLE WT cells at 25-32 SPCC584.13,VIABLE WT cells at 25-32 SPCC584.14,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPCC584.15c,VIABLE WT cells at 25-32 SPCC584.16c,VIABLE WT cells at 25-32 SPCC594.01,VIABLE WT cells at 25-32 SPCC594.02c,VIABLE WT cells at 25-32 SPCC594.04c,VIABLE WT cells at 25-32 SPCC594.05c,VIABLE small colonies long cells at 25-32 SPCC594.06c,VIABLE WT cells at 25-32 SPCC594.07c,VIABLE WT cells at 25-32 SPCC5E4.03c,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC5E4.04,ESSENTIAL germinated spores long at 25-32 SPCC5E4.05c,VIABLE WT cells at 25-32 SPCC5E4.06,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC5E4.07,VIABLE small colonies WT cells at 25-32 SPCC5E4.10c,VIABLE WT cells at 25-32 SPCC61.01c,ESSENTIAL spores at 25-32 SPCC61.02,VIABLE WT cells at 25-32 SPCC61.03,VIABLE WT cells at 25-32 SPCC61.04c,ESSENTIAL spores at 25-32 SPCC61.05,VIABLE WT cells at 25-32 SPCC613.01,VIABLE WT cells at 25-32 SPCC613.02,VIABLE WT cells at 25-32 SPCC613.03,VIABLE WT cells at 25-32 SPCC613.04c,ESSENTIAL germinated spores long misshapen- lysis at 25-32 SPCC613.05c,ESSENTIAL spores at 25-32 SPCC613.06,VIABLE small colonies slightly misshapen- vacuolated cells at 25-32 SPCC613.07,VIABLE WT cells at 25-32 SPCC613.08,VIABLE small colonies slightly misshapen cells at 25-32 SPCC613.09,ESSENTIAL microcolonies small cells at 25-32 SPCC613.10,VIABLE WT cells at 25-32 SPCC613.11c,VIABLE WT cells at 25-32 SPCC613.12c,VIABLE WT cells at 25-32 SPCC622.01c,VIABLE WT cells at 25-32 SPCC622.08c,VIABLE WT cells at 25-32 SPCC622.09,ESSENTIAL spores- germinated spores at 25-32 SPCC622.10c,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC622.11,VIABLE WT cells at 25-32 SPCC622.12c,VIABLE long cells at 25-32 SPCC622.13c,ESSENTIAL microcolonies small cells at 25-32 SPCC622.14,VIABLE WT cells at 25-32 SPCC622.15c,VIABLE WT cells at 25-32 SPCC622.16c,VIABLE WT cells at 25-32 SPCC622.17,VIABLE WT cells at 25-32 SPCC622.18,VIABLE small colonies WT cells at 25-32 SPCC622.19,VIABLE WT cells at 25-32 SPCC63.02c,VIABLE stubby cells at 32 slightly misshapen cells at 25- misshapen cells at 36 SPCC63.03,VIABLE WT cells at 25-32 SPCC63.04,VIABLE WT cells at 25-32 SPCC63.05,ESSENTIAL spores- germinated spores at 25-32 SPCC63.06,VIABLE WT cells at 25-32 SPCC63.07,ESSENTIAL spores- germinated spores at 25-32 SPCC63.08c,VIABLE WT cells at 25-32 SPCC63.10c,ESSENTIAL microcolonies slightly rounded cells appear to be dark edged at 25-32 SPCC63.11,ESSENTIAL germinated spores slightly long at 25-32 SPCC63.12c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPCC63.13,VIABLE WT cells at 25-32 SPCC63.14,VIABLE WT cells at 25-32 SPCC645.02,VIABLE WT cells at 25-32 SPCC645.03c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC645.04,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC645.05c,ESSENTIAL germinated spores long- often branched at 25-32 SPCC645.06c,ESSENTIAL spores- germinated spores slightly misshapen- some division at 25-32 SPCC645.07,VIABLE WT cells at 25-32 SPCC645.08c,VIABLE WT cells at 25-32 SPCC645.09,ESSENTIAL microcolonies skittle cells- small colonies WT cells at 25-32 SPCC645.10,ESSENTIAL germinated spores at 25-32 SPCC645.11c,VIABLE WT cells at 25-32 SPCC645.12c,VIABLE WT cells at 25-32 SPCC645.13,VIABLE WT cells at 25-32 SPCC645.14c,VIABLE stubby septated cells at 25-32 SPCC663.01c,VIABLE very small colonies rounded cells at 25-32 SPCC663.02,VIABLE WT cells at 32 SPCC663.03,VIABLE WT cells at 25-32 SPCC663.04,VIABLE small colonies slightly misshapen cells at 25-32 SPCC663.05c,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC663.06c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPCC663.08c,VIABLE WT cells at 25-32 SPCC663.09c,VIABLE WT cells at 25-32 SPCC663.10,VIABLE WT cells at 25-32 SPCC663.11,VIABLE slightly misshapen cells- often long at 25-32 SPCC663.12,VIABLE slightly misshapen cells at 25-32 SPCC663.13c,VIABLE WT cells at 25-32 SPCC663.14c,VIABLE WT cells at 25-32 SPCC663.15c,VIABLE WT cells at 25-32 SPCC70.02c,VIABLE WT cells at 25-32 SPCC70.03c,VIABLE WT cells at 25-32 SPCC70.04c,VIABLE WT cells at 25-32 SPCC70.05c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC70.06,VIABLE WT cells at 25-32 SPCC70.07c,ESSENTIAL microcolonies long cells at 25-32 SPCC70.08c,VIABLE WT cells at 25-32 SPCC70.09c,VIABLE WT cells at 25-32 SPCC70.10,VIABLE WT cells at 25-32 SPCC736.02,VIABLE WT cells at 25-32 SPCC736.03c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC736.04c,VIABLE slightly misshapen cells at 25-32 SPCC736.06,VIABLE skittle cells at 25- microcolonies skittle cells at 32 SPCC736.07c,VIABLE small colonies long slightly misshapen cells at 32 SPCC736.08,VIABLE small colonies misshapen cells at 32- cells slightly misshapen at 25 SPCC736.09c,VIABLE WT cells at 25-32 SPCC736.10c,ESSENTIAL microcolonies skittle cells at 25-32 SPCC736.11,VIABLE WT cells at 25-32 SPCC736.12c,ESSENTIAL microcolonies misshapen cells at 25-32 SPCC736.13,VIABLE WT cells at 25-32 SPCC736.14,VIABLE slightly misshapen cells at 25-32 SPCC736.15,VIABLE WT cells at 25-32 SPCC737.02c,VIABLE WT cells at 25-32 SPCC737.03c,VIABLE WT cells at 32 SPCC737.05,VIABLE WT cells at 32 SPCC737.06c,VIABLE WT cells at 25-32 SPCC737.07c,VIABLE WT cells at 25-32 SPCC737.08,ESSENTIAL spores- some germinated spores at 25-32 SPCC737.09c,VIABLE WT cells at 25-32 SPCC74.01,ESSENTIAL spores at 25-32 SPCC74.02c,VIABLE slightly misshapen cells at 32 SPCC74.03c,VIABLE WT cells at 25-32 SPCC74.04,VIABLE WT cells at 25-32 SPCC74.05,VIABLE small colonies WT cells at 25-32 SPCC74.06,VIABLE WT cells at 25-32 SPCC74.09,VIABLE slightly misshapen cells at 25-32 SPCC757.02c,VIABLE WT cells at 25-32 SPCC757.03c,VIABLE WT cells at 25-32 SPCC757.04,VIABLE WT cells at 25-32 SPCC757.05c,VIABLE WT cells at 32 SPCC757.07c,VIABLE WT cells at 32- cells slightly misshapen at 25 SPCC757.08,ESSENTIAL spores- germinated spores- often long at 25-32 SPCC757.09c,VIABLE slightly misshapen cells at 25-32 SPCC757.10,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC757.11c,VIABLE WT cells at 25-32 SPCC757.12,VIABLE WT cells at 25-32 SPCC757.13,VIABLE WT cells at 25-32 SPCC777.02,VIABLE WT cells at 25-32 SPCC777.03c,VIABLE WT cells at 25-32 SPCC777.04,VIABLE WT cells at 25-32 SPCC777.05,ESSENTIAL spores- germinated spores- microcolonies slightly misshapen cells at 25-32 SPCC777.06c,VIABLE WT cells at 25-32 SPCC777.07,VIABLE WT cells at 25-32 SPCC777.08c,VIABLE WT cells at 32 SPCC777.09c,VIABLE WT cells at 25-32 SPCC777.10c,ESSENTIAL microcolonies long cells at 25-32 SPCC777.11,ESSENTIAL microcolonies skittle cells at 25-32 SPCC777.12c,VIABLE WT cells at 32 SPCC777.13,VIABLE WT cells at 25-32 SPCC777.14,ESSENTIAL microcolonies long curved misshapen cells at 32- microcolonies with some long cells at 25 SPCC777.15,VIABLE WT cells at 32 SPCC777.17c,VIABLE WT cells at 25-32 SPCC790.02,ESSENTIAL small colonies misshapen cells at 25-32- small colonies rounded cells at 36 possibly gives rise to suppressors SPCC790.03,VIABLE WT cells at 32 SPCC794.01c,VIABLE WT cells at 32 SPCC794.02,VIABLE WT cells at 25-32 SPCC794.03,VIABLE WT cells at 25-32 SPCC794.04c,VIABLE WT cells at 25-32 SPCC794.06,VIABLE WT cells at 25-32 SPCC794.07,VIABLE small colonies WT cells at 25- small colonies WT cells with some long cells at 32- very small colonies swollen long cells at 36 SPCC794.08,VIABLE slightly stubby cells at 25-32- cells seem to die rapidly in stationary phase SPCC794.09c,VIABLE slightly misshapen cells at 32 SPCC794.10,VIABLE slightly misshapen cells 32- some dead cells SPCC794.11c,VIABLE WT cells at 25-32 SPCC794.12c,VIABLE small colonies rounded cells at 32 SPCC794.15,VIABLE WT cells at 25-32 SPCC825.01,VIABLE very small colonies slightly misshapen cells at 25-32 SPCC825.02,VIABLE WT cells at 25-32 SPCC825.03c,ESSENTIAL spores at 25-32 SPCC825.04c,VIABLE WT cells at 25-32 SPCC825.05c,VIABLE WT cells at 32 SPCC830.03,ESSENTIAL microcolonies slightly misshapen cells at 25-32 SPCC830.04c,VIABLE WT cells at 25-32 SPCC830.05c,ESSENTIAL germinated spores slightly misshapen- some division at 25-32 SPCC830.06,VIABLE WT cells at 32 SPCC830.07c,VIABLE WT cells at 25-32 SPCC830.08c,VIABLE WT cells at 32 SPCC830.09c,ESSENTIAL microcolonies small cells at 25-32 SPCC830.10,VIABLE WT cells at 25-32 SPCC830.11c,ESSENTIAL microcolonies slightly misshapen cells- some long cells at 25-32 SPCC895.03c,ESSENTIAL microcolonies slightly misshapen septated cells at 32- cells slightly curved at 25 SPCC895.04c,ESSENTIAL spores- germinated spores at 25-32 SPCC895.05,VIABLE small colonies misshapen cells at 25-32 SPCC895.06,VIABLE small colonies long cells at 25-32 SPCC895.07,VIABLE curved cells at 32- WT cells at 25- misshapen cells at 36 SPCC895.08c,VIABLE WT cells at 25-32 SPCC895.09c,VIABLE WT cells at 25-32 SPCC962.01,VIABLE WT cells at 25-32 SPCC962.02c,ESSENTIAL germinated spores misshapen at 25-32 SPCC962.03c,ESSENTIAL germinated spores at 25-32 SPCC962.04,VIABLE WT cells at 25-32 SPCC962.05,VIABLE WT cells at 32 SPCC962.06c,ESSENTIAL germinated spores slightly misshapen and divide once at 25-32 SPCC965.03,ESSENTIAL spores at 25-32 SPCC965.04c,VIABLE WT cells at 25-32 SPCC965.05c,VIABLE WT cells at 32 SPCC965.06,VIABLE WT cells at 25-32 SPCC965.07c,VIABLE WT cells at 32 SPCC965.08c,VIABLE WT cells at 32 SPCC965.09,VIABLE WT cells at 25-32 SPCC965.10,VIABLE WT cells at 25-32 SPCC965.11c,VIABLE WT cells at 25-32 SPCC965.12,VIABLE WT cells at 25-32 SPCC965.13,VIABLE WT cells at 25-32 SPCC965.14c,VIABLE WT cells at 25-32 SPCC970.01,VIABLE WT cells at 25-32 SPCC970.02,VIABLE WT cells at 32 SPCC970.03,ESSENTIAL spores- germinated spores at 25-32 SPCC970.04c,ESSENTIAL microcolonies rounded cells at 25-32 SPCC970.05,VIABLE WT cells at 25-32 SPCC970.06,VIABLE slightly misshapen cells at 25-32 SPCC970.07c,VIABLE WT cells at 25-32 SPCC970.08,ESSENTIAL microcolonies rounded cells at 32 cells slightly misshapen at 25 SPCC970.09,ESSENTIAL germinated spores misshapen- some division at 25-32 SPCC970.10c,VIABLE long branched septated cells at 25-32 SPCC970.12,ESSENTIAL microcolonies misshapen cells at 25-32 SPCP1E11.02,VIABLE WT cells at 25-32 SPCP1E11.03,VIABLE WT cells at 25-32 SPCP1E11.04c,VIABLE slightly misshapen cells at 32 SPCP1E11.05c,VIABLE WT cells at 25-32 SPCP1E11.06,VIABLE slightly misshapen cells 32- some dead cells SPCP1E11.07c,VIABLE WT cells at 32 SPCP1E11.08,ESSENTIAL spores- germinated spores at 25-32 SPCP1E11.09c,VIABLE WT cells at 25-32 SPCP1E11.10,VIABLE small colonies WT cells at 25-32 SPCP1E11.11,VIABLE WT cells at 32- cells slightly misshapen at 25 SPCP20C8.01c,VIABLE WT cells at 25-32 SPCP20C8.02c,VIABLE WT cells at 25-32 SPCP25A2.02c,VIABLE WT cells at 25-32 SPCP25A2.03,ESSENTIAL microcolonies long- weak skittle cells at 25-32 SPCP31B10.02,VIABLE WT cells at 25-32 SPCP31B10.03c,VIABLE small colonies long curved cells at 25-32- aberrantly septated SPCP31B10.04,VIABLE WT cells at 25-32 SPCP31B10.05,VIABLE WT cells at 25-32 SPCP31B10.06,VIABLE WT cells at 25-32 SPCP31B10.07,VIABLE WT cells at 25-32 SPCP31B10.08c,ESSENTIAL germinated spores at 25-32 SPCPB16A4.02c,VIABLE WT cells at 25-32 SPCPB16A4.03c,VIABLE WT cells at 25-32 SPCPB16A4.04c,VIABLE WT cells at 25-32- cells stubby at 36 SPCPB16A4.05c,VIABLE WT cells at 25-32 SPCPB16A4.06c,VIABLE WT cells at 25-32 SPCPB1C11.01,VIABLE WT cells at 25-32 SPCPB1C11.02,VIABLE WT cells at 25-32 SPCPB1C11.03,VIABLE WT cells at 25-32 SPCPJ732.01,VIABLE WT cells at 25-32 SPCPJ732.02c,VIABLE WT cells at 25-32 SPCPJ732.03,VIABLE WT cells at 25-32