ID SPCC548 standard; DNA; FUN; 18591 BP. XX AC AL359685; XX SV AL359685.1 XX DT 21-JUN-2000 (Rel. 64, Created) DT 24-JUL-2000 (Rel. 64, Last updated, Version 2) XX DE S.pombe chromosome III cosmid c548. XX KW conserved hypothetical; gene-free region; glucose transporter (x2); KW highly similar to ght5; similarity to yeast urm1; tf1-type LTR; KW ubiquitin-related; wtf-11 pseudogene; wtf13; zf-C3H4C zinc finger. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-18591 RA Brown S., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (19-JUN-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O74850; O74850. DR GOA; Q9P3U6; Q9P3U6. DR GOA; Q9P3U7; Q9P3U7. DR GOA; Q9P3V0; Q9P3V0. DR SPTREMBL; Q9P3U7; Q9P3U7. DR SPTREMBL; Q9P3U8; Q9P3U8. DR SPTREMBL; Q9P3U9; Q9P3U9. DR SPTREMBL; Q9P3V0; Q9P3V0. DR SWISS-PROT; O74850; YCYF_SCHPO. DR SWISS-PROT; Q9P3U6; GHT1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c548 is overlapped at the 5' end by cosmid c1235, EMBL entry CC SPCC1235, accession number AL031764 and at the 3' end by cosmid c1529, CC EMBL entry SPCC1529, accession number AL132664. XX FH Key Location/Qualifiers FH FT source 1..18591 FT /chromosome="III" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c548" FT /map="IIIL" FT misc_feature 1..101 FT /note="nominal overlap with SPCC1235 AL031764 S.pombe FT chromosome III" FT CDS join(1..147,228..548) FT /codon_start=1 FT /db_xref="GOA:O74850" FT /db_xref="SWISS-PROT:O74850" FT /label=SPCC548.01 FT /note="SPCC548.01, len:155, SIMILARITY:Caenorhabditis FT elegans., O01881, cosmid k07b1., (339 aa), fasta scores: FT opt: 475, E():2.3e-26, (50.0% identity in 140 aa)" FT /partial FT /gene="SPCC548.01" FT /gene="SPCC1235.15" FT /product="hypothetical protein; similar to yeast YOR245c" FT /protein_id="CAB94943.1" FT /translation="IGGAQESLLSRPGQNNLVLKKRFGFVKLAFLTGSSLVPCFAFGES FT GKIEDIFEQVDNNPRTRIYKFQEIVKKIAGFTVPFFYGRGLLNKTFGLMPWRKPINIVV FT GEPIDVPKKSHPTNQEIYEVHEEYIRRLEGLWNKYKDVFLPNRISELKLSA" FT LTR complement(1980..2280) FT /note="Tf1-type LTR" FT CDS join(complement(3786..3921),complement(3372..3617), FT complement(3047..3322),complement(2932..3006), FT complement(2823..2899)) FT /label=wtf11-pseudo FT /note="SPCC548.02c, len:269, SIMILARITY:Schizosaccharomyces FT pombe, O74838, hypothetical wtf protein., (258 aa), fasta FT scores: opt: 1201, E():0, (74.4% identity in 270 aa)" FT /pseudo FT /gene="wtf11-pseudo" FT /gene="SPCC548.02c" FT /product="wtf11-pseudo no Met" FT misc_feature complement(2900..2913) FT /note="ctaattatttttag, splice branch and acceptor" FT misc_feature complement(2926..2931) FT /note="gtactt, splice donor sequence" FT misc_feature complement(3007..3018) FT /note="ctgacaacatag, splice branch and acceptor" FT misc_feature complement(3041..3046) FT /note="gtttgt, splice donor sequence" FT misc_feature complement(3323..3334) FT /note="ctaactcattag, splice branch and acceptor" FT misc_feature complement(3366..3371) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(3618..3630) FT /note="ttaatttaaacag, splice branch and acceptor" FT misc_feature complement(3780..3785) FT /note="gtaaga, splice donor sequence" FT CDS join(complement(6204..6339),complement(5703..5975), FT complement(5364..5654),complement(5136..5327), FT complement(5033..5095),complement(4837..4973)) FT /db_xref="GOA:Q9P3V0" FT /db_xref="SPTREMBL:Q9P3V0" FT /label=wtf13 FT /note="SPCC548.03c, len:363, SIMILARITY:Schizosaccharomyces FT pombe, O74420, hypothetical wtf5 protein., (418 aa), fasta FT scores: opt: 1154, E():0, (82.8% identity in 418 aa)" FT /gene="wtf13" FT /gene="SPCC548.03c" FT /product="hypothetical wtf protein" FT /protein_id="CAB94945.1" FT /translation="MKNKDYPLRSSMDELSTKNDNEIDLEKGPLPEYNSEDGSTLPPYS FT EIWKYIKTVSEDSSTGPTEIANPNVERRQEFKDSHPNIYSLLRLLISVLAVIVVFFTAW FT VCVNPLEKSIFGKVAFSVTIGITCPIVFIVIFCFFETWTQAVAQCIKVTVIFLAQCVKV FT TAVFLAKCVKVIAVGLYNSKKDLVVTIWLAWVVICFILFGCVKDGRLNLNKALICSTCS FT ISAALFFILLLVCIPIWTLKHMLFGLFQVLGVQSCVVIVTKGLMYLFDKHIDATGYEIE FT ASSLFVIGNFLFFYEMERPGALKRMPKFIGNGIASFLGNAFGGIGNAFGGIGNAIGRIG FT NAFRGANDNNDIPLGEMDVESEV" FT misc_feature complement(4974..4995) FT /note="cttacccgactaggaggtttag, splice branch and acceptor" FT misc_feature complement(5027..5032) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(5096..5108) FT /note="ctaaccataccag, splice branch and acceptor" FT intron complement(5096..5135) FT /note="confirmed by mRNA" FT misc_feature complement(5130..5135) FT /note="gttcgt, splice donor sequence" FT misc_feature complement(5328..5338) FT /note="ctaacatacag, splice branch and acceptor" FT intron complement(5328..5363) FT /note="confirmed by mRNA" FT misc_feature complement(5358..5363) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(5655..5666) FT /note="ctaactcattag, splice branch and acceptor" FT misc_feature complement(5697..5702) FT /note="gtaagc, splice donor sequence" FT misc_feature complement(5976..5988) FT /note="ttaatgctttcag, splice branch and acceptor" FT misc_feature complement(6198..6203) FT /note="gtaagt, splice donor sequence" FT CDS join(7023..7045,7089..7241,7325..7490) FT /db_xref="SPTREMBL:Q9P3U9" FT /label=SPCC548.04 FT /note="SPCC548.04, len:113, SIMILARITY:Saccharomyces FT cerevisiae, YIA8_YEAST, hypothetical 11.0 kd protein in FT faa3-mas3 intergenic region., (99 aa), fasta scores: opt: FT 292, E():5.5e-14, (44.2% identity in 95 aa)" FT /gene="SPCC548.04" FT /product="putative ubiquitin-related protein; by similarity FT to yeast urm1" FT /protein_id="CAB94946.1" FT /translation="MAIKVELLGGLDLLFNKQKALSLSLSNLGSTKLGSLIDYMAQIIE FT KPSQKDLFILNGTVRPGIIVLVNDQDWELLEKEEYNLEEGDEVVFVSTLHGKTIFSIFF FT IIFKTNCIN" FT misc_feature 7046..7051 FT /note="gtaggt, splice donor sequence" FT misc_feature 7072..7088 FT /note="gtaacctattattgtag, splice branch and acceptor" FT misc_feature 7242..7247 FT /note="gtaagt, splice donor sequence" FT misc_feature 7308..7324 FT /note="ctaacacttccctgcag, splice branch and acceptor" FT CDS join(complement(8961..9281),complement(7725..8810)) FT /db_xref="SPTREMBL:Q9P3U8" FT /label=SPCC548.05c FT /note="SPCC548.05c, len:468, SIMILARITY:Caenorhabditis FT elegans., Q22463, similar to zinc finger containing FT protein. ncbi gi: 1049400., (2447 aa), fasta scores: opt: FT 235, E():1.7e-06, (25.1% identity in 355 aa)" FT /gene="SPCC548.05c" FT /product="zf-C3H4C zinc finger protein" FT /protein_id="CAB94947.1" FT /translation="MVKRSSHRQVVLDEDDEENYNNNLDDEKMEVLLIPQSNSTTFASS FT DATQMYKKSRISSNSENKKQIPDTKTLLETFQKIKKTLECPICTEALQRPFTTHCGHTY FT CYECLLNWLKESKSCPTCRQKLYTQPSPAYLVYEIMNVVAASNSGFPLVGINENPAKKQ FT KEVLFDGMFKQEDSHYPRSILVDSEDGVLRCARCQWELENPYHCDHCGFQISDDQDSGR FT EWFWDGENAESDSSLNGDNTRGGNISTNRAFNNMGHAPITAVPQDWLRFDEGEEFVGSD FT LESDFSGPGEYDVDDGFIDNRATSQLSPVESDDDFVAPVNGSNGNGITALDSTDSEEID FT IMNGFDEERDSGGTNMVSRSETCYNDGQRYDELRRELADIQNESLDSLNSSSNNSPSHN FT NIHSRQHPFSSDEDEGNIVTNGTGLRSSQSSSQNRGFDLEPYSEVFTASYPRRQARRTR FT TIQLDSDEES" FT misc_feature join(complement(8961..9029),complement(8766..8810)) FT /note="Match to PF00097 zf-C3HC4, Zinc finger, C3HC4 type FT (RING finger) Score 35.78" FT misc_feature complement(8811..8821) FT /note="ctaataaatag, splice branch and acceptor" FT misc_feature complement(8955..8960) FT /note="gtaagt, splice donor sequence" FT CDS complement(10395..12038) FT /db_xref="GOA:Q9P3U7" FT /db_xref="SPTREMBL:Q9P3U7" FT /label=SPCC548.06c FT /note="SPCC548.06c, len:547" FT /gene="SPCC548.06c" FT /product="putative glucose transporter protein; highly FT similar to ght5" FT /protein_id="CAB94948.1" FT /translation="MGKTLTIVMLVFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYN FT PVTDTYSYSSARQGLITGMVNVGSMTGCILSSPLMDRIGKRVSIMFWTIVYLIGIILQV FT TAVPSWVQIMVAKIWTGLAIGALSVLAPGFQSEVAPATLRGTIVTTYQLAVTGGIFIAA FT CINMGTHKLHKTAQWRVSMGINLLWGIIMFIGISFLPESPRYLIAIGKDEEALDIMCKN FT NVLPREHEIIQTEYHVIKTDCEAEMAGGPATWGDILGADIRYRTFLGLGVMSLQQLTGD FT NYYFYYGFEVFEGTGMNSPYLSALILDAVNFGCTFGGLFVLEFFGRRMPLIIGGVWQSI FT TFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRY FT RSKCAAVATTGNWLWGFLITFFTPFISDSIGFKYGYIFAACNLCAACIIFLFAHETKGL FT TLEEINELYLSGAKPWMPRPENLGQAAKQQQEVLEKSRGVQGESAAHLENVDNEGMEDT FT SSNDITSSTSSSEGRAKPESNYVDEQDRYA" FT misc_feature complement(10638..12008) FT /note="Match to PF00083 sugar_tr, Sugar (and other) FT transporter Score 491.66" FT misc_feature 12039..16471 FT /note="gene free region" FT misc_feature complement(16189..16309) FT /note="region in 3' UTR 97 % conserved between pombe ght1 FT S.pombe GHT1 gene" FT CDS complement(16472..18145) FT /db_xref="GOA:Q9P3U6" FT /db_xref="SWISS-PROT:Q9P3U6" FT /label=ght1 FT /note="SPCC548.07c, len:557, SIMILARITY:Schizosaccharomyces FT pombe, P78831, putative glucose transporter protein., (546 FT aa), fasta scores: opt: 2842, E():0, (78.3% identity in 529 FT aa)" FT /gene="SPCC548.07c" FT /product="putative glucose transporter protein." FT /gene="ght1" FT /protein_id="CAB94949.1" FT /translation="MSKTLTITMLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYN FT PVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQV FT TAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAA FT CINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKN FT AELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGC FT NYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSI FT CFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRY FT RSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESKGL FT TLEEINELYLSDAKPWMSRPKNLSEMAEAKEVEREETRPKGESARYLEHADSNSDSEDL FT SSSANNDVHEPVARQDPFNENEVEKSRTRPSQESYTTSSN" FT misc_feature complement(16745..18115) FT /note="Match to PF00083 sugar_tr, Sugar (and other) FT transporter Score 499.31" FT misc_feature 18491..18591 FT /note="nominal overlap with SPCC1529 AL132664 S.pombe FT chromosome III cosmid c1529." XX SQ Sequence 18591 BP; 6320 A; 3331 C; 3452 G; 5488 T; 0 other; atcgggggag ctcaagagtc tcttttgtcc cgtccaggtc aaaacaatct tgtacttaaa 60 aagcgtttcg ggttcgttaa gttagctttt ttaacaggat ccagtttagt cccttgcttt 120 gcttttggag agtctggtaa gattgaattt tagtattttt agatagctta aaaagtcgtc 180 gaattacttt gatgttgttt actttagtat cttgctaacc aacacaagac atttttgaac 240 aggttgataa caatccgcgt actcgtatct acaaatttca agaaattgtc aaaaaaattg 300 caggatttac agtccccttt ttctatggtc gtggactatt aaacaaaact tttgggttga 360 tgccttggcg taaacctatt aacatcgttg ttggagagcc catcgatgtt cctaaaaagt 420 ctcaccccac aaatcaagaa atctatgagg ttcatgaaga gtacatccga agacttgaag 480 gcttatggaa caaatacaaa gatgtgtttt taccaaatcg tattagcgaa ttgaagttgt 540 cagcctaaat taatatgaag aagtcttcac ataaaataag agcagtttct tattggccta 600 tgcaatatgt tgtgagattt tatatcatta acgatttctt cttttccttc tttagagcag 660 tttataagct atatttattt tgcaaaaatt tacaatataa tgtttgaggc actttagact 720 tgtttatttc tgttcccttc ttcaattaaa tgtaaaggcg gttgctttca tgcaatcgtc 780 ttatcatgct aagtaaaaat catgcagcaa gccatgagac gataatttcg tttgtgacta 840 aaagacgacg cagcagtatg caatagtata taaattagat ggcatacttg gcggggagta 900 ttaaatcgaa ggcatgggct ttgccgcctt tattaaataa ttcagtaagt cttagcgatc 960 gtttacattt gatgagttga cagggtttgt aaattcaaac aaagagtaaa agttacagcc 1020 ctttgaggta tctgatcagg tttaatattg gtacactgca tgctataaag gataaatgga 1080 aattatcttt gtcttactga ttcataggta ttcacaatta cttcaagcgt tactaatatt 1140 cattcagtaa ttagaagaaa cgatttttat tggaggcatt ttcacaaatg gttcgaaggt 1200 tcagacctgc aataagttta attaattcaa gcaaccatgt acgcccaatg tttcctattg 1260 tctaattgga aattaaagtg agtgtttttt aacggaattt caagtcgtgc tttactaaca 1320 aaattcctta tatgaggaac gaggaattta ctatataagc atttttaaat tgttcgcaga 1380 ttcggttcta aaaaaaaaag aggccatttc ttcttagcta ttgcttttct agtattaacc 1440 caattactgt agtttcgaaa acgagcaaaa aggtctataa aacaagtgta gggagtcgat 1500 ctcagactgc aaatcgctaa atttcaaaat ttgataaggg tagagtcttg tgagaaggaa 1560 aatagaattg tcgaatattt caatagcatc cttaaaccaa agttaaaaaa aagtatatat 1620 atcaaactaa aagcgtacct gctaattgct tgaagcattc gatgttcaac tcataagctt 1680 tatggacaca ctctacgatg gttaagcatg tgatcttcat acgacgcttt gcttcccgca 1740 aaatcaatat aactattaaa agatgtatag agtgatgggt gtagtactct aaacatactg 1800 gaatagatta tatgtgaact aaggaacatt cgaatagatc atcttcatag caaaatattc 1860 atttggcagt taattaaaat gtttatgaaa ttacaagatg atcaatagta attatgctca 1920 agaataggct caaagatgaa gaggtgcata atatagtgta aagcactatg actcttgtgt 1980 tcgaatttca ttaacaatac taccctatgt acgttacgtt gtcaccgata gatgtaatgt 2040 ttgcaaatcc gttgtattca agtcaccaag gcaaactatt tactacatgt gtcaaaacaa 2100 acaatataaa aatattataa atagagctcc tactgaactg aggaacgaag ttcagtcata 2160 tgctatatta gggataggta tgttatactc agttgctact catactacct gcgtatcgca 2220 atataataga tcacaaggaa acccaacaca gttctatgta ttgttaaaac aaataccaaa 2280 ctgcgtagct gacatgttat tgcgataaca tttcgtctat ctaagtcaca atattttatt 2340 atgctgttca ttattttgta aacattgaaa tttgtttgaa ttgaaccagt aaacagaaga 2400 aaaaatgatg attaagcttg attcattaag aatcaggatg aaagagaaat gaatgcaata 2460 aacgacaatg aaattgtgaa tacaacaagg aggaacagcg agttttccaa tttcattagt 2520 tgatgataac agtcaattag tgaactgcta ttttaaaacg aataaataaa accattattt 2580 taatattcaa ttcccagtgt atgttatcca acatcgcaga atctttcaag caatgcactt 2640 gctcatccat ttcgtcagga aatgaaatca tgcaataaaa atacatctga aatactctaa 2700 acacatcatg atttgtttgg taaatcacaa ttatatagat gcttctttcc attgcacatt 2760 cttgtagaga agtttcgtga gaaaagcgcc tttataaatc aatagtaaag ttagcaatat 2820 aattaggctt cattttcagg gttcatctca cgtaacggaa tagcagtgta ttcattcacg 2880 tgttgtctgg catcacgatc taaaaataat tagtaaacac atttaaagta cacttctttt 2940 tatcatcttt accgcaagtt gttgcaagtc tggtcagtaa atgcgtagag caagatcccg 3000 gttccactat gttgtcagta ttaattttgt ttagtaaaca acaaacctgt ttcagcaatg 3060 aatatgagaa tgcttgatac agccatgaaa gtgaaagcgg atgccgagca tttccatccc 3120 cattgttgat cacctaaatg tgaactcaat ttgtttacac cttttaataa aaccaagcaa 3180 aaaatagaac ttttacaaaa agcaaaaaac atgtttctcc acttctttaa aaagtgcttg 3240 attccttttc cgcaagcctt gacacattgt gccaagaaaa ttacagttac tttaactgcc 3300 ttggtccaaa tttcatagaa gtctaatgag ttagtgactg aaggtaagaa taaccccatc 3360 gtctcactta caagtcaaga atattaaagc caaagtgcaa acaagacacc aaaatccaaa 3420 taatgtccat tccgctgcac cataattttt aaaaaaagcg tccttatact ttagataaca 3480 aactgctgga gcattgaata gaatgattga agtgaatgat attagcagtt ttatcaacaa 3540 aggggaagaa ttatctgtcg acctggaagg attgttttct ctatgtgtgt taggagaatt 3600 gcttaaacgt gcatgatctg tttaaattaa tatgattgaa agactttagt taagaaaaag 3660 catgtatcaa agtgtgtgca ttgcatggcc aactgtaaac aatgaccaaa gcatgcaatt 3720 tgactacgat ggcaagcttc aactttcaat tgaataattc ctttcttatt atttacaatt 3780 cttacctgaa tatggaggca atgtactttc ctcctctgaa tcatattctg ggaggggtcc 3840 tttttccaaa tcaatttcat gatctgtctt caattcatct tcttcatcta tagaactttt 3900 caaggaagtg taattattct tcgtttcatc aagataattc tttatttaac aaaacacatg 3960 cgcgaattaa ttgctatgat tgcgacaaac gaaacagcaa aatcaagaat attcactctg 4020 gagttcagtt cccgccgttc aacataaata aatgagcaat agtcgtttca aagcaaactg 4080 ttttttcaat aatagcaagt gaaaagtaaa attccaaaac tcattttcaa aatgtaattg 4140 cttctgacac atttattttg tcactgccct tcttattcac cccaacttag atttccttat 4200 gcatcattaa gaaactcaac ataaaaccag tatatacgtt tactgcgtta ttacatactc 4260 actattgcaa tatgtcttta ggttattacg ataacaaatc atcgttctga gtcacaatat 4320 tttattatgc tattcattat ttcgtaaaca ttgaaatttg tttgtattaa agtaaaccat 4380 taaacaaatg gaaaaaatga taattaagct tgattcatta agaatcaaga tgaaggagaa 4440 atgaatgcaa taaacgaaaa tgaaattgtg aatttaacaa ggcggaacaa cgagttttcc 4500 gatttcatta gttgatttta agttgtcgta aacaagttaa accattattg tttgtagcgt 4560 agagtatcat taaaaaagac acataattag actcgcatac ataataaata aagacttcgg 4620 tcctcaatgc acattatcca acatcgtaga atgcattggt tggcaatttt tggtaattga 4680 aagaatgagt taagcttata tctcacaaac ttataaggca agagaataat taaagtatgt 4740 tggtgcttat ttttaaaaaa cgccgcagct tctctgcgtt ggtgattttg gtaaagaggt 4800 tttccaactg gagcgacaac cacattaata aagcaattag acttcgcttt caacatccat 4860 ttctcccaag ggaatgtcat tattatcgtt ggcacctcta aacgcatttc ctatccgccc 4920 aattgcattt cctatccctc caaatgcatt tcctatccct ccaaatgcat ttcctaaacc 4980 tcctagtcgg gtaagtaaaa aatcattcat acagttggtt gaatacacat acctaggaaa 5040 gaagctatac cattcccgat aaatttgggc atccttttga gagcacctgg acgttctggt 5100 atggttagag taaattacag gaatatataa cgaacccatt tcgtaaaaga aaagaaaatt 5160 tcctataaca aagagtgaag aggcttcaat ttcgtagcct gtcgcgtcga tatgtttatc 5220 aaataaatac atcaaacctt tcgttacaat aacgacacaa ctttgtacac ccagcacttg 5280 aaacaaacca aaaagcatat gcttgagtgt ccaaattgga atacaaactg tatgttagcg 5340 tttagcaaga taatttcact taccaagcaa taaaatgaaa aataaagcag ccgaaatact 5400 gcatgtggaa cagatgagtg ccttatttaa gtttagtcta ccatccttga cgcaaccaaa 5460 gagtataaaa caaatgacta cccatgcgag ccagattgta accaccaaat ctttttttga 5520 gttatacaaa ccgaccgcaa ttacctttac acattttgcc aagaaaactg cagtcacctt 5580 tacacattgt gccaagaaaa ttacagtcac ctttatacat tgtgccactg cctgggtcca 5640 agtttcaaag aagcctaatg agttagtgaa tggacatatt aacaacttca tgcatagctt 5700 acagaaaata acaataaaca ctattggaca tgttatacca attgtgacgg agaaagctac 5760 cttgccgaat atcgacttct ccaacgggtt tacacaaacc catgcagtaa aaaagacaac 5820 aataactgca agaactgata ttagtagtct cagaagggaa tatatatttg gatgtgaatc 5880 tttgaattct tgtcttcttt cgacattggg atttgcaatt tctgtaggtc ctgtacttga 5940 atcctcagat acagttttaa tatatttcca aatttctgaa agcattaata aaataaataa 6000 cttttgaaat ttggtttttt ttttttaaaa tgatgtagaa ggaagaaagt gtgtgccctg 6060 ttagacgaat ttatgctcgt aatatgtcaa gttgtaaaca aacaaataca acaaaaacat 6120 tgtaaatcaa aacaaacaga cacagcaaat aatccttgac acactcaatt ctttatctta 6180 tccaccaaca acacaacact tacctgaata tggaggcaat gtgctcccat cttctgaatt 6240 gtattctggg agaggacctt tttccaaatc aatttcatta tcgttttttg tactcaattc 6300 atccatagac gacctcaagg gataatcttt attcttcatt tcttagaaac aatctattat 6360 tgaatgatat acacacaaat agttcaattg ttgtgattgc gacaaacgaa acagcgaaat 6420 caagaatatt cactctgtag tccaattcca accactttaa agacttttta acaaaataat 6480 aaaattgttc aaacaaattt ccaactaatt ccttcaaaca atggcataaa aaaagaagaa 6540 cgaaaaaact gaacgaggca gtggattgct tctgacacat ttattttgtc actgcctttt 6600 ttattcaccc caacttagct ttccttaaat gtaagctacg cagtttggta tctgatttac 6660 ggatacgtag aactgcggtg aggtttttag taatttcaat atagcggtat gcgccctgtt 6720 aactaacaat attatattta ttgttataag aagtctaata gtatcgaata ccaatatatc 6780 taataactaa actgaggaat cgagtcagca gttgttatca acgggtattc ataatacggt 6840 ggttagcgtg tgacatataa tgatacataa cgtagtgagt cataaaacac gctaaacaga 6900 taccaaactg cgtagctgac atgacactga atttctatca ctctttaaca actacagtgg 6960 aatatacagt tagaacgtac attttgcgtt gctttcacga atccccaaag ttttaaaaaa 7020 caatggctat aaaagtagag ttactgtagg tgtcatattt tattacaatt ggtaacctat 7080 tattgtagag gtgggcttga cttgctcttc aataagcaga aagcgttgtc gttgtcactt 7140 tcaaaccttg gctcaactaa gttgggatca ttgattgatt atatggctca aataatagaa 7200 aagccttctc aaaaagattt atttatttta aatgggactg tgtaagtgaa catggagtgg 7260 tatcatcttt ttcaggctca aacgatgttt taaatgattt tgcagaacta acacttccct 7320 gcagacgtcc ggggatcatc gtcttagtaa atgaccagga ttgggaactt cttgaaaagg 7380 aagaatataa cctcgaagaa ggtgacgaag ttgtattcgt ctcaacgtta cacggtaaaa 7440 caatatttag catttttttt ataatattta aaactaattg tatcaactag gaggttaaat 7500 ggcaggtata attataagac aaaacaaaca tgcacaaata tcattagtat tgaatttttt 7560 tttttagtca taaatccgac atgtatgcta aattttagtg tcttatgctc tagagaccca 7620 aaatccaaat cacaattaaa tcattcatca tgaacagaaa ccatagacca ggttaaggtt 7680 acttgatttc tcggtataat tttttcttcg ttagagttga gtaattatga ttcttcgtca 7740 gagtctagtt gtattgtacg agttcgccga gcttgccgac gtggatagct tgcagtgaat 7800 acctcggaat agggttcaag atcaaatcct ctattctggc tggatgattg tgaactacgg 7860 aggcctgttc cattagttac aatatttcct tcatcctcat ctgatgagaa tggatgttgc 7920 ctggaatgaa tattgttatg agaaggagaa ttatttgagg aagaattgag agaatccaaa 7980 ctttcattct gaatgtcagc aagctctctt cgtaactcat cgtatctttg cccatcgtta 8040 taacaggtct cagatcgaga taccatattc gtccctccag agtcgcgctc ttcatcgaag 8100 ccgttcataa tatcaatctc ttcactatct gtgctatcca aagcagtaat accatttcca 8160 ttacttccat tgacaggagc tacgaagtca tcgtcacttt cgacaggaga taattggctt 8220 gtagcacgat tatctataaa tccatcatca acatcgtact cacctggacc agaaaaatca 8280 gattctaaat cagaccccac aaattcttct ccttcgtcaa atctcagcca atcttgtggt 8340 actgcagtta ttggcgcatg tcccatatta ttaaatgcgc ggttagtcga gatattccca 8400 ccacgggtgt tatcgccatt taggctggaa tcactctcag cattttctcc atcccaaaac 8460 cattcacgac cagaatcttg gtcatccgat atttgaaaac cgcaatgatc gcaatggtac 8520 ggattttcca attcccattg gcaacgagcg catcgtaaaa ctccatcttc agaatcaaca 8580 aggatacttc ttgggtaatg agaatcttct tgtttaaaca tcccatcaaa caatacctcc 8640 ttttgcttct ttgctggatt ttcattgatt cccacaagtg ggaagccact gtttgaggca 8700 gcaaccacat tcattatttc atatacaaga tatgctggtg aaggttgtgt atacagtttt 8760 tggcgacaag ttgggcaaga ttttgactct tttaaccaat ttagaagaca ctatttatta 8820 gtttcaatcc tttatagaaa aaaagtaaat ctagaaactt ttaaagatat aatcttgctc 8880 tttcaatatt tcactcacaa tgagggaatt caatgataat gaaaagttat tccagtgtta 8940 agcatcaaat tttcacttac ctcgtaacaa tatgtatgac cgcaatgagt ggtaaaaggt 9000 ctttgtaaag cttctgtgca aatgggacat tccagagttt tttttatttt ctggaacgtt 9060 tcgagcaaag tttttgtatc ggggatttgt tttttatttt ctgaattact gctaatgcga 9120 gactttttgt acatttgagt tgcatcacta gacgcaaaag ttgtgctgtt ggattgtgga 9180 attaatagaa cctccatttt ctcatcgtcc aagttattat tataattttc ttcatcgtct 9240 tcatccaaaa ctacttggcg atgagaactg cgtttaacca ttgcgcccag acagataaat 9300 tactattgaa cttggtatgt gagctctaac gtttctaatc cggagagaaa gctttatcaa 9360 aataataagt ttaaataaaa tcctttaaaa aaacgaatta tatgcaaatt cttcaaagca 9420 aatcaaccat tgatttgtac tttatatatt tcttgtagat ggcaaatact ctgcgtattg 9480 caatgcagcg gtttacaact gcattatcaa attcttctgg tattatcata aatatcattt 9540 agtgacttgt agtcagtgta atctcgggca tatgtaaatt tatttaacgt tacaaaatgc 9600 gattgcgttc aacaagcatg gctaatagcg ttttcttgta gcttggattt ttatgaggat 9660 tattcacaga tacgcaccga tggcatttca cgtatagttt taagtataat attttcaacc 9720 atagctttca atgtttcact ctaagtagaa ttgaacgtct cccgacttaa ctcgtaaata 9780 tcgttgatta cttaccggaa accttttttt gcatcgttct aaccaggtaa ataaattgaa 9840 cgaatgctat tcaaaaacat tcaaggatta aataacatat caacaattag atactacgct 9900 tatttactgt tcagcatacc tttctactca atgtctacaa ttcgcaatac acaaaacgat 9960 atgtaatagc acattagaaa taaggaactg tttaattttt gttaatcctt aacgaaaaaa 10020 ctatcagcat ttaactaaat tatctgttta cattattatt aaaattataa tgcaatccct 10080 gccagttcct catcaacgcc tttccatagt aataaggacc gggccactca taaaaacatt 10140 accagttaaa aagcatgcac accaacttaa tattaatatc tctaacagaa aggacggaac 10200 agtttgaata acaacgacaa aggaaatgca aataaaaaat aactctcaaa gttggcacag 10260 ccaaaatcca attatacaat tcgattttaa tgaaggatga atcagcaaaa aacacatact 10320 cattaaaaag aaataacatt taaaaactac cgattcgtgt aaaccgaaag cattgccatt 10380 ttcggtcaac aaacttatgc gtagcgatct tgttcatcaa catagttaga ctcaggctta 10440 gcacggcctt cagaagaaga ggtagaagat gtaatgtcgt tactcgaagt atcctccata 10500 ccctcattgt caacgttttc aagatgagcg gcactttcac cttgaacacc acgagatttt 10560 tcaagaacct cttgttgttg ctttgcagct tgtccgagat tctcgggcct aggcatccag 10620 ggcttagcgc cggagagata cagttcgtta atctcttcca aggtaagacc tttggtttca 10680 tgggcaaaca agaaaataat acaagcagca cacaaattac aggcagcgaa aatataacca 10740 tacttgaatc caatggagtc actaatgaag ggagtaaaga aagtaataag gaaaccccac 10800 aaccagttac cagtggtagc gacggcagca cacttggaac gataacgaat gggatacgat 10860 tcaccaacga taacgtatgc ggcaggaccc caagtttgag caaaactgaa aatgaaaaga 10920 catgagaaca caatcataac ggcaccggca cgatggtttg aggtaccatt cttacgagta 10980 agagcacgat taccaacagc tgcgtaaatg aagaaagtaa tggattgcca aacaccacca 11040 ataatcaatg gcatacgacg accaaagaac tctaggacga aaagaccacc aaacgtacat 11100 ccaaagttaa cggcatcaag aatcaaagca gaaagataag gagagttcat accagtaccc 11160 tcaaacacct cgaaaccata gtagaagtag tagttatcac cggtaagttg ttgaagagac 11220 atgacaccaa gaccaaggaa ggtacgataa cgaatatccg cacctagaat gtcaccccaa 11280 gtagcgggac caccggccat ttcagcttca cagtcagttt taatgacatg gtattccgtt 11340 tgaataattt cgtgttcacg aggaagaacg ttgtttttgc acataatgtc aagagcctct 11400 tcatctttac caatagcaat caaataacga ggggattcag gaaggaaaga aataccgatg 11460 aacatgataa taccccaaag caagttaata cccatggata cacgccattg agcagtcttg 11520 tgcaacttgt gagtacccat gttgatacaa gcggcaatga aaataccacc agtgacagcc 11580 aattgatagg tggtgacaat ggtaccacga agggtagcgg gagcaacctc agattggaaa 11640 ccgggggcaa gcacagacaa ggcaccaatg gcaagaccag tccaaatctt agcaaccatg 11700 atttggaccc aagatgggac agcggtaact tgaagaataa taccaatcaa ataaacaata 11760 gtccaaaaca taatggaaac acgcttgcca atgcgatcca taagaggaga agacaaaata 11820 caaccagtca tagaaccaac gttaaccata ccagtaatta aaccttgtcg cgctgaagaa 11880 tacgaatacg tgtcagtcac cggattatac cgatctgcga aacgagattg gaaatctctc 11940 atgttggtga taccaccaat ggaaccggta tcggcgccaa acatccatcc ggccatggac 12000 acgaagacca acatgacaat ggtcaatgtc ttacccattt taagtaaatt cgaatactaa 12060 aaatataggg cacaaaaaat aatatgtaat gatgtgattg aaactttatt aaataataaa 12120 cgtttcaaaa tctattttaa aaagatgaga tttcacaaag ctctatgttg caaaagtgaa 12180 gtatgtagta ttaatgtaaa gggaatgtgg ataatatgaa ccagcgaaat taaatagaaa 12240 agaatagagg ttttttaaag gattaaataa ccgcaaaagg atgagggaag gggtgtatga 12300 aaatgtgatt aatggatatg ctagagcgtt ccaaacaaga aaaacgaaga cgctttaaaa 12360 aataaaagag tgggttaaaa aaaaggaaag ccgaaggaac tgaaaccccg tcgaacaaaa 12420 gttaaattaa ccgtaaacca cagattttac taagtaaaac gtacaatttg gagcagaagc 12480 tgtagaaggc tactcaagtg agcgggctac ttatatagaa aaaaaataca tgcaataatg 12540 gatgtggggc gcttgcgggg ttgcggggag tgtccttgac agcgtagcta aatcgattca 12600 cgtaggctag acaccgtaca tgcaggtcta ctattggtga agagaggaag caattatgct 12660 cagttttgca ctcccaaatg cggaaccatt tatgacgaga atggcatcca aggccgaggt 12720 tgaataactg aaagctctgt cggctccctt aaggcaaaca tctaacacta aagaagtcga 12780 atacaaaggt attacaccca caaaataata acgaatcata gtcaaaacta ccaaggaatg 12840 acccacagta gggatgatcc agcactgcac agtaaaaaac ttacagacga acctctgcta 12900 ctttctacca aatgtcacat tgatagcaaa actgtccata ttgtacttgt gacaaccatc 12960 gaacgtgaag aacggactag gtaagctgaa gagggtaatg aaagggtctc gaccagtatt 13020 ttgatcctgg cttgcgtggc cggtgaaacc ctatccagtt ggtgtaccta taagcgtcta 13080 atattggaaa aagccaagtg gagagggctg taaactagtc tcgtgcaaac gtgagaaaat 13140 gcggagaaaa tgacgactcc ggcacttcct gccgttagtg ggaaagatag gatggtcaaa 13200 ttagtacggc actattccga atgcaccata actgtccact ccgccagtga ggtacattaa 13260 aaaagtagat tgcaacgacg attgcaacgt tggttgctat gccgttgctt tacgaggtgt 13320 agtgtatgcc aatgctaaga ggaaatggca agagtggaga cttttgcggt aaaggtagat 13380 taaagagatc tattagacgc atccttacaa aatcttatcg tagttcgtag ggaggcgata 13440 taagatgaat tgattttcta gttgttgttg ttgttgttca actgtactgc aagccgctaa 13500 ggtaggccaa ggatgaaccg tgtaaagact cgtaaagata cctataagga agagagggaa 13560 gataatagtt aagagagaga agagtataca acgatagtta tatcactatt gagaaataac 13620 agagcgtagt aacccgagac aagtataaac aaaccagaaa atgatatgaa gagatgagac 13680 gagatgatcc aaaaaggaaa aaggaaaaat tgtttcatta tcctttatct ttttttaaaa 13740 aaaatgcatt tacgcgtttt taatagaatc atgttttaat ggacctatcc aatacttttg 13800 aaacgctctt tttctttcac aatcgcgtcc accaggtttc cagacaaaca atcctcattt 13860 cacgcatacg cacacgtact acacatctta ccgtgtattg ccagacgtgt taaatccctt 13920 catgataaga gtaaagacca aaagttgctt tcatttacac cgtttttcat acattaatgt 13980 aaggtcaatt tcaatttgac atcttctcgt tataacgaac actcatccta cagcacgaat 14040 tgttggacac acaatatgcc atccactttc gaggatgtga gaacccgtac tgtaggaggg 14100 aagacctcct tttatatgaa agaagatggg gtacacagat ggcgaatacc tgggtggtta 14160 aagtttcctg cactcccctc aaaatgaggg cacccactca acgaggcata aatgacaaga 14220 gactatgtag cggcatttga caaattagca gatttcgcac cttgattttg ctaattatat 14280 gcctcgttaa tcggcgttta acctttttct tggaagtttg gtacttggtt tcccttgggc 14340 ccatgcgatt gattagtagc tcatcgttaa ttcgtctttt tgggtgggga atattgcgga 14400 gtctaccaat gaagcgcagt caagggcaat acaagtagga aaagtagtgc ggtaggaatc 14460 tctctattcg ttgattaagg aggggaagag gggtagcgac gaaaagtggc atttgttgat 14520 ttccttgagc ataagagcga aaagctaaga aaacgcttgc taagtgtgaa gggtttataa 14580 gcactgcaga atggagaatt gattattgtg aaatttttag agaaatttca atagctctac 14640 aaagaaatat ccaccctttt cgatatcaaa gcaaacgtcc atccctcaag aaaaaaatcg 14700 cacacattcc tagactacat tagacaaaac atctccactt ttaccgccaa ttttgataat 14760 gaaaaaagca caattcgtac tgtggactgt ccagattcct taattagcag agaaaaaagt 14820 ggggaaacga tgggaaaagc ggtttaaggt ggaagaacgc agccgcgtaa ttcgtcttgc 14880 tgcgatacag gtatagagaa ggaagtagga aggaaaggag aagaatagca aagggaaatg 14940 aatgcaaagg taaagttgaa agagtgttta taaggagata aaaagaaaca aaataaaatt 15000 agaaaaaatt gactaggaga tttttagatc gaaaatatta ttattgctat tctaattatc 15060 agcggtttgt ttgttttttt ttttcggctt cattttaggg ttgtgtgtcg gcttgcggaa 15120 gcacgtgtat taacggtgcg aaaaaaaaaa aacactggga atttttaata agccctgtaa 15180 aatgtctgta tcttgaatta agacagtggt ttgagacaat ggtttttttc aagcatacta 15240 ataaacatga gtctttatac tgttcagaaa tgaaccttta tgctgttaac agtgaatatg 15300 ttttatgttt cgaaaatact tttcgctata acactaataa aagtccataa aacgctcatt 15360 atgagtcgtg gattcagaga ggctgcttct ttcccaccat gttttgtata tttcaattgg 15420 gtaaaggcaa gtctgttttg aaaattgtaa aggctaaaat gaaatattct agtatggatt 15480 tattaacgtt ggatccttta aagttggttt cttatatttt attttatttt ttagtttttc 15540 tttaatttaa ttgttttttt ttttggggga tatttatttt gtttatttat ttatttttaa 15600 attcctatcg ttttatttaa ttgtatcaat tgttaatatt ctttttttct aaattttatt 15660 atgtatataa attcatttta caataccatt cacaatttac aaaaatttac ctcttacaaa 15720 aaaactgctg ctcatttact agttatttta atattttttt tttttttttc tctttttctt 15780 actaatttta ggttcactct actctcccaa tgtaccgtgg atttttattg cctttagcta 15840 ttaatttcta tattttacag agtcgtacat ctacaaaagt tgttgcactt tatgtgtaaa 15900 ttatcgctaa agtgaaagaa taattcttgt attattttga atgaaaatca attttataca 15960 tacgagcaaa gtagaaatga tacattatta aacggctaat tatggttgtt ggaaagattg 16020 gaagaggtag ggaaaggaat tgagagaaat aatagaacga gattttcgcg tatttcgatg 16080 gattgatcgg ccagcttgat tcacttggaa atcggtttag cttttttaga ctgaaaaatc 16140 gtactacaat tgaaattgag ttttactttg catgaaatag gacattccaa atttcaagtc 16200 gtgttcttta tggctttctc cttatggcgt gaacgagcat ggctaaggaa acgcaggttc 16260 aggcgtggcg aatgtgggtc ggaattgtaa acatccttaa agcagaaaac ggaatcaaac 16320 aaaaaacaat taaatacagg tgtgtaaaag agtaaacaga attttcatag aggaacaaaa 16380 ggatgagaag gtgaaaataa ggaaaatgtg aggaaataaa aagtaaaaaa aaatttaaaa 16440 aaaattttaa accaaaacaa tcaaaccgaa attagttgga ggaggttgtg tatgattctt 16500 ggcttggtcg ggtacgagac ttttccactt cattttcatt aaatggatct tggcgggcaa 16560 caggctcatg aacgtcgttg ttcgcactgg aagagaggtc ttcagaatcg gaattggaat 16620 cagcgtgctc caaatatctg gctgattcgc cctttggacg agtctcctcg cgctccactt 16680 ctttggcttc tgccatttcc gacaaattct tgggacgtga catccagggt ttggcgtcag 16740 agagatagag ttcgttaatt tcttccaagg taagaccctt ggactcgtga gcaaacaaga 16800 acgttacgat ggaggcacaa aggttgcacg atgcaaagat gtaaccatac ttgaatccaa 16860 tggagttggt gataaatgga gtgaagaagg aaatcaagaa attccaaagc caattactag 16920 ttgtcgcaac ggcagcacac ttggaacgat aacgaatggg atacgattca ccaacgataa 16980 cgtatgcggc aggaccccaa gtttgagcaa aactgaaaat gaaaagacat gagaacacaa 17040 tcataacggc accggcacga tggtttgagg taccattctt acgagtaaga gcacgattac 17100 caacagctgc atagatgaag aaacaaatgg attgccaaac accaccgatg atcaatggaa 17160 cacgacgtcc gcaatattca agtacgaaaa gaccaccaaa cgtacatcca aagttaacgg 17220 catcaagaat caatgaggcc aagtaaggag attccatacc ggttcccttg aacacttggg 17280 taccgtagta gaaataatag ttgcaaccgg tcaattggtt aaaggacata atagccattc 17340 ccaaaaatgt tctgtatctg atttccttac caaaaatctc tggccatgtg catggaccac 17400 cctcgacggc ggcttcacag tccgacttga tttgcttgta ttccgtttga atgatgtccg 17460 aatcgggagg aagctcagcg ttcttacaca taacctggat tgcctcatca tccttaccaa 17520 tggacacaag ataacgagga gattccgaca agaatgagac accgacaagg gtgatgatac 17580 cccagagcaa gttaataccg atggatacac gccattgagc agttttataa agtttgtggg 17640 tacccatgtt gatacaagcg gcaatgaaaa taccagctgt aacacacaat tgataggtaa 17700 cgacaatggt accacgaata tggggaggag ccacttcaga ttggtaaccg ggagcaagca 17760 cagacaaggc accgattcct agaccggtcc aaatcttagc aaccatgatt tggacccagg 17820 atggaacagc ggtgacttga atgataacgc cgatgaggta aataacacag aacccaataa 17880 tggaattacg cttaccaatt cgatcagtag tgggagaaga aattaaacag ccgaagaaag 17940 aaccaacgtt gaccatacca gtaattaaac cttgccgcgc tgaagaatac gaatacgtgt 18000 cagtcaccgg attataccga tctgcgaaac gagattgaaa gtctcgcata ttggtgatac 18060 caccaatgga acccgtgtca gcaccgaaca tccatccggc catggacaca aacaacagca 18120 tcgtaatggt caatgtctta ctcattttgc cttaggtaaa gagaagaaag gaagaaggaa 18180 agaaaaaaag accaacaacg atgttgtgaa gttaaaggat attttgtttt taagactttg 18240 taggctctgc ctgaagaatt cctaagtatt tgaaaatagg aatgtaattc tcaataggaa 18300 gagagaaaaa gaaagtcgcg aatttaagtg agaaaagaga agaaaaaaca aatagaaaat 18360 gaaaaaaaaa gagtttcttt attggaatta tttctttggt attgttttgt tttgttttgt 18420 tttgtttttt tcaaaatcct attttcgttc gatcaaatgc aagacaaaaa ttcctagtat 18480 cttgtgaaaa gatcgtaaac aaatgggtat gcgattatac tgcttccacg cttacgcttt 18540 tacgcttacg attgttggat tggaataggc agttgaatga gagagaatgg g 18591 //