ID SPCC10H11 standard; DNA; FUN; 6758 BP. XX AC AL162771; XX SV AL162771.1 XX DT 30-MAR-2000 (Rel. 63, Created) DT 30-MAR-2000 (Rel. 63, Last updated, Version 1) XX DE S.pombe chromosome III cosmid c10H11. XX KW 5s rRNA gene; DEAD/DEAH box helicase domain; DEAD/DEAH box RNA helicase; KW DNAJ protein. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6758 RA Saunders D., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (30-MAR-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9P7C6; Q9P7C6. DR GOA; Q9P7C7; Q9P7C7. DR SPTREMBL; Q9P7C7; Q9P7C7. DR SWISS-PROT; Q9P7C6; CWFN_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c10H11 is overlapped at the 5' end by cosmid c970, EMBL CC entry SPCC970, accession number AL031530 and at the 3' end by CC P1 p31B10, EMBL entry SPCP31B10, accession number AL121859. XX FH Key Location/Qualifiers FH FT source 1..6758 FT /chromosome="III" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c10H11" FT /map="IIIL" FT misc_feature 1..95 FT /note="nominal overlap with SPCC970 S. pombe chromosome 3" FT misc_feature 393..441 FT /note="short region of similarity to a gene free region of FT SPCC1840 S. pombe chromosome 3" FT CDS 1540..4584 FT /db_xref="GOA:Q9P7C7" FT /db_xref="SPTREMBL:Q9P7C7" FT /label=SPCC10H11.01 FT /note="SPCC10H11.01, len:1015, SIMILARITY:Rattus FT norvegicus, Q62780, rna helicase., (1032 aa), fasta scores: FT opt: 2117, E():0, (41.7% identity in 1073 aa)" FT /gene="SPCC10H11.01" FT /product="DEAD/DEAH box RNA helicase" FT /protein_id="CAB85446.1" FT /translation="MSRRTRSRSPPRRSYNRERRDYRKYDDSEDQKGARYNRYVDDVSS FT RRDRHDSFRSHESNKIRRDNSWKHDKYSYEKRYQERDREYARSKNIPDQYIGRSPRPTS FT HRHAEEKEVDNKTKSDETNPVLQGSATEIKPQPRRSRFDRTERVGASLSVSEIQSENPR FT VDVIPKDKAVENNHQRNAEKPVASDKITDAKLLARLERVRAWKESKAKQEASKKEEHKL FT NTKPQVTAKDQNAMPSTGISGFEINRQKDTSDMKRNNRVHMDDEDGPRRMNLEDYQELW FT DQEDRGMLGNEQAASMEEDEVDPLDAYMASLVGTTDTIRPGLLNTEVIDPNANDDERMV FT ISETLEEEENLLALAAKRSKKKDVITVDHSKINYEDFKKDFYVEPEELKNLSPAEVDEL FT RASLDGIKIRGIDCPKPVTSWSQCGLSAQTISVINSLGYEKPTSIQAQAIPAITSGRDV FT IGVAKTGSGKTIAFLLPMFRHIKDQRPLKTGEGPIAIIMTPTRELAVQIFRECKPFLKL FT LNIRACCAYGGAPIKDQIADLKRGAEIVVCTPGRMIDVLSANAGRVTNLHRCTYLVLDE FT ADRMFDLGFEPQVMRIINNIRPDRQTVLFSATFPRAMEALARKVLKKPVEITVGGRSVV FT ASEVEQIVEVRPEESKFSRLLELLGELYNNQLDVRTLVFVDRQESADALLSDLMKRGYT FT SNSIHGGKDQHDRDSTISDYKAGVFDVLIATSVVARGLDVKSLQLVVNYDCPNHMEDYV FT HRVGRTGRAGHTGVAVTFITPEQEKYAVDIAKALKMSKQPVPKELQTLASQFLEKVKAG FT KEKAAGGGFGGKGLSRLDETRNAERKMQRKAYGEDEEDVETEAEAKSPLEKITPEKSTG FT DPTLDRVRAAVGGIAARAFANQTAQSNKLTQPISIIKTDGDEYKAKMEINDYPQQARWA FT VTNNTNIVHVTELTGTSITTKGNFYLPGKNPEPGEEKLYLWIEGPSELVVNRAITELRR FT LLLEGINHSLEGGNKPSASGRYTVV" FT misc_feature 2836..3465 FT /note="Match to PF00270 DEAD, DEAD/DEAH box helicase Score FT 223.27" FT misc_feature 2923..2946 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 3205..3255 FT /note="PS00237 G-protein coupled receptors signature" FT misc_feature 3256..3282 FT /note="PS00039 DEAD-box subfamily ATP-dependent helicases FT signature" FT misc_feature 3583..3828 FT /note="Match to PF00271 helicase_C, Helicases conserved FT C-terminal domain Score 104.07" FT misc_RNA 5289..5475 FT /note="5s rRNA gene" FT CDS 6132..6758 FT /db_xref="GOA:Q9P7C6" FT /db_xref="SWISS-PROT:Q9P7C6" FT /label=SPCC10H11.02 FT /note="SPCC10H11.02, len:209, SIMILARITY:Homo sapiens, FT BAA91724, cdna flj10634 fis, clone nt2rp2005654, weakly FT similarto cysteine string protein., (304 aa), fasta scores: FT opt: 376, E():3.3e-13, (34.5% identity in 203 aa)" FT /partial FT /gene="SPCC10H11.02" FT /gene="SPCP31B10.01" FT /product="DNAJ protein" FT /protein_id="CAB85447.1" FT /translation="MASEGDSIDYYELLGINEDAQDQEIHRAWRKTSLKYHPDKNPNDP FT KAAEKFHMLQLAYNALIDVQLRKAYDSERFAKLARKRREEAFNFQRKSMVDDLRERERQ FT FYDSLEKKENERDRLQEKLRALQEESANLRRQRENRLREEQEQSKRRKQETPSSKISEL FT DRSIRIRWKRKYADQVNDAYLRSIYSSFGTLQNVVIQKDISKEKKY" FT misc_feature 6156..6347 FT /note="Match to PF00226 DnaJ, DnaJ domain Score 92.54" FT misc_feature 6282..6341 FT /note="PS00636 Nt-dnaJ domain signature" FT misc_feature 6660..6758 FT /note="nominal overlap with SPCP31B10 S. pombe chromosome FT 3" XX SQ Sequence 6758 BP; 2306 A; 1131 C; 1319 G; 2002 T; 0 other; ggaataatca actctatttc tatttccgtt cacaaatgtg ttaaataaaa aacagtagca 60 tgagatagac tccttcttag gaatttaggc ggatcgtgaa gttaagagac tctcaaggat 120 gatgaaagga agaatctgta caaaacaaaa atcctatata agacaatgaa tttaaaaatg 180 attaccaact gataaaagta tccaaggaga accaaataca aatatttaac ctaaagatga 240 aagagtgaat ggaatacagc aatcagtaaa aatgagcaac caactataca taccaactag 300 aggttaaact agtttcaata tattgggtaa aataaattac ttaatatact ctttaaccat 360 tgctcagttt cgggcatagt tcatgcctta gcttaattct ccttaattag atactctaca 420 ttcaatcttt ccatgtattc acgttggggt atcccatcgc ttgtatttgg acaaccatat 480 tttttaaatt ttctttcaaa atgtaagatt tatttgctgt cggtttctaa cttcattaca 540 tgcgtataac cattgctcat cctaaaaaca attatcccag tattaagtat atcaaattat 600 aaagcaatac cagaaaaata atgccagcta ttaatactat cactgttaaa tcgagtaaat 660 ggcgaaaaaa tacacttaaa aaaaataaaa ataaatatac acataaataa ttttctcttt 720 catttggcat tactaaagaa ttttcgtttt ttggttttct gctaagtcaa tattattggt 780 cgaagagcgc aaaaacataa acattataga aaaagtataa cattatgtaa atttcaatta 840 tgcttttttt tttttctttt ttggaatgga aataatggct tttcttaatt tatgtacgct 900 ctaaattatc tgacttatta cattctattg tagatgactg tttaacagac atttgaaaga 960 gtaccttact agtaatcaag aattgtggat tccaagcctt aaccccaata gttatttaca 1020 taaaaaaaga aatattgtaa ataaaaagag ctaactcgcc tattcgccaa tcaataaaat 1080 tttttatcgt cttttatttc tcggttttcc gatattatct ttagtacaaa agcaataaaa 1140 attcagcaac aatcaaaatg taagcggtgt tgaaaaaatt acttatatac cagtgttgat 1200 tatttttccg agttttactg gttaagttga aggtccagtg tagggaacca taattaaatt 1260 ttaaataact gaaataaaaa atgaatatta cttctaagat ttataaattg cacagaattc 1320 aggttgcttt accgacttaa ctggcaaatt cattgctttt taataaaccc catatgactt 1380 gtataaataa tcataaagag gttaactata ataatgtaaa ttatatctag tagtgaacta 1440 atatcatatg cataatatat attgctatat aaagtaattt atgtcgttta cttgcctcat 1500 taaaactctt cgtgtacata tcacaacacc cagtttttga tgtctagaag aacacgatca 1560 aggtcgcctc caaggcgatc ctacaataga gaaagaagag attaccgaaa atatgatgac 1620 tctgaagatc aaaagggtgc acggtataac cgatacgtag acgatgtatc aagtcgacga 1680 gataggcatg attcttttcg ttctcacgag tctaataaaa ttagaagaga taattcttgg 1740 aagcatgata agtatagtta cgaaaaaagg tatcaagagc gagatcgaga gtatgcgaga 1800 tcaaagaata ttcccgatca gtatattggt aggtcaccgc gaccaacatc tcatagacat 1860 gctgaggaaa aagaagtaga caataaaact aaatcagatg aaacaaatcc tgtattacaa 1920 ggttccgcaa ctgaaataaa gcctcagcct agacgttctc gatttgatcg aactgagaga 1980 gttggtgctt ctttaagtgt ttcagaaatt caatctgaaa atccacgagt tgatgtaatt 2040 cccaaggata aagctgttga aaataatcat caacgaaatg ctgaaaagcc agtagcgtca 2100 gacaaaatta cggatgctaa gctactcgcc aggctagaac gagttagagc gtggaaagag 2160 tcaaaggcaa aacaggaggc ttccaagaag gaggagcaca agttgaatac aaagccgcag 2220 gttacagcca aagaccagaa tgctatgcct tcaactggta tatccgggtt tgagattaat 2280 cgccaaaaag atacttcgga tatgaagcga aataaccgag ttcacatgga tgatgaagat 2340 ggtcctaggc gtatgaatct tgaagactac caggagcttt gggaccaaga agaccggggt 2400 atgctaggaa atgagcaagc agctagcatg gaggaagacg aagtagatcc tttagatgct 2460 tacatggcct ctttggttgg taccactgat accataaggc ctggtttatt aaatacggag 2520 gtcattgatc cgaatgcaaa cgatgacgag cgcatggtaa tttcagagac tttggaagag 2580 gaagaaaatt tacttgctct tgctgcaaaa cgatcaaaaa agaaagacgt aatcacagtt 2640 gatcactcaa aaataaatta tgaagatttc aaaaaggatt tctatgttga acctgaagag 2700 ctcaaaaatc tgtcaccagc tgaagttgat gaactacggg cttctttaga tggtattaaa 2760 attcgtggta ttgattgccc gaagccagtt actagttgga gtcaatgtgg tttatctgcc 2820 caaaccataa gcgtgatcaa ttccttggga tacgaaaaac caacctctat tcaagcacaa 2880 gcaattccag cgattacttc tggcagagat gtaattggcg tagctaaaac aggaagtgga 2940 aaaaccattg cttttcttct tcctatgttt cgacacatca aagatcagcg cccattaaaa 3000 acaggagagg gtcctattgc aataataatg actcctactc gtgaattggc tgtgcaaatt 3060 tttcgcgaat gtaaaccttt cttaaagtta ttgaacattc gtgcgtgttg tgcatatgga 3120 ggtgccccta ttaaagacca aattgctgat ttaaaacgcg gtgcagaaat cgtagtttgt 3180 actcctggac gtatgattga tgttttaagt gcgaatgcag gacgtgttac taacttacac 3240 cgctgcactt atttggtttt ggatgaggcc gatcgtatgt ttgatttagg atttgaaccg 3300 caagtcatga gaattattaa caatattcga cctgatcggc aaaccgtact attttcagct 3360 acctttcctc gtgctatgga agcccttgca aggaaagttt taaagaaacc tgtagagatt 3420 acggttggcg gacggtcggt tgttgcttct gaggttgagc aaatcgttga agtacggcct 3480 gaggaaagca agttttctcg gctattagag ctgttaggtg agttgtacaa caatcaactt 3540 gatgtacgta cacttgtttt tgtggataga caagagtctg cggatgcact tttgtcggac 3600 cttatgaaac gcgggtatac aagtaattca attcatggcg gtaaggatca acacgatcgc 3660 gactcaacca tcagtgatta taaagcagga gtatttgatg tattaatcgc aactagtgta 3720 gttgctcgtg ggcttgacgt taaatcttta cagctggtgg taaattatga ttgccctaat 3780 catatggagg attacgtgca tcgtgttggt cgtacaggcc gtgctggcca tactggtgta 3840 gctgttacct ttataacccc agaacaggaa aagtacgccg tagacattgc caaggcttta 3900 aagatgagta aacaaccagt tccaaaagaa ctacaaactc ttgcttccca attcctcgag 3960 aaagttaagg ctggtaaaga gaaagcagcg ggtggaggtt ttggcggcaa agggttatca 4020 cgactggatg aaactcgaaa tgctgagcgt aaaatgcagc gtaaggccta tggtgaggac 4080 gaggaagatg tagagactga agctgaagca aaaagcccgt tggaaaagat tactcctgaa 4140 aaatctaccg gtgatcccac gctcgataga gttcgtgctg ctgtgggtgg aattgctgct 4200 cgtgcatttg ccaaccaaac tgcacaatct aacaagctta ctcagccaat ttctataata 4260 aaaaccgatg gtgatgagta taaagctaaa atggaaatta atgactatcc tcaacaagct 4320 cgttgggccg ttactaataa cacaaatatt gtacatgtaa cggagttaac tggcacaagt 4380 atcactacta agggtaattt ctacttgcca ggtaaaaacc ctgaaccagg agaggaaaag 4440 ttgtacttgt ggattgaagg tccatctgag cttgttgtta accgagccat cactgaattg 4500 agacggcttc ttcttgaagg aattaaccac agtttggaag gaggtaacaa accaagtgct 4560 tctggacgat acacagttgt ctagtacttt cggtctatcc ttttgaaaaa gagacctaca 4620 ctcgtttaaa attaatcaat tgaatacgtt acgtgctttt ttggggaagg gcaaaagtgg 4680 ttatgaaaaa ttaatgatta gaaatgtcaa tatttattat tttaactaaa catatatgga 4740 ttttggcatg aaaattaagg catgtatctt ttttcatcgc aaagtaaaag cgggaaagaa 4800 tgattttatt cacattttta aagcgagtac ttttccaact gctttcctga aatagtgtta 4860 gggtatttgc tttttgtaat ccacagtatt tatagtaaac aataaactaa tggttgaatt 4920 taatcttttg tgaagagaag aaataaaatg taatttttgg ccttcaatta tcagtgctaa 4980 acacaaatag taggcaatca atcccatagt tgtttatttg agaaaaaggt tctgtcagtg 5040 aatgcttgat ttcatgattg atgggtttaa aagaagttaa aattataaaa atcagaattt 5100 tttagcgtag aagtaaaaac cttcaattat ttaatttttc tccgactatt tgcataagac 5160 ctaaacaaac taactgtatg cgaactatat tgtactaaca ctgcatcaag ttatataatt 5220 acaatttcga tgtacattgt attaaatgaa tgcaaaaatt tatttttttt actattttct 5280 tttttattat aaagggcatt aaagtaaacg tagattgtct acggccatac ctaggcgaaa 5340 acaccagttc ccgtccgatc actgcagtta agcgtctgag ggcctcgtta gtactatggt 5400 tggagacaac atgggaatcc ggggtgctgt aggctccttc ttttctttct tttttgcctt 5460 tttaatcctg tttttacatt tgttaattat ttaataggtg cgctgaccta aacacaactg 5520 cgtctcatat gcggcaaagt gcaatgtctt attttcatga attatataca ctacttattt 5580 tagcgagata ttaaacaaaa cttgagtagc tcatagaaag ttagctaaga ttacctttta 5640 tctttttttt gaagttattt gttctagcta tagttcatta actacaggca aaaatttacc 5700 tcttttctgg cttctaatat ttttttcgct taaaataact caaagtcaat aagcagcgaa 5760 aaattacgta tacacaagag aatggcgctg aaccttgagc cgcagtatag taggtaccgc 5820 aagcaactaa acatcgttat agtagtagag cagttggaat ttaagcgcgc taaaattatt 5880 aataatatat cctaacgtgg agtgttgtag tcaagagatt agttattccc aagtactgat 5940 tttgttcgaa aaagaacaat tctcgagatc gaaaagaact gttgtacgag gttcgttggg 6000 actgcagtat aactttatgc tttttcattt ttccctttac aattctttag aagcaaggaa 6060 cacagcactg ttagaaaaca cgacggtgag agaagtgctt cttttttgaa agattccaat 6120 tctcttgaaa aatggcatct gaaggtgatt caattgatta ctatgaactg ttaggtatta 6180 atgaagatgc tcaagatcaa gaaattcatc gcgcttggcg aaaaacttcg ttaaaatatc 6240 atcctgacaa aaatccaaat gatcccaagg cggcggaaaa gtttcacatg cttcagttag 6300 catataacgc gctaattgat gtgcaattaa gaaaagcata tgactctgaa aggtttgcta 6360 agcttgctcg caaaagacgc gaggaagcat ttaactttca gcgaaaaagt atggtagatg 6420 acttgaggga aagagaacgc caattctatg actctttaga gaagaaagaa aacgaaagag 6480 atcgacttca agaaaaactg agggctttac aagaagaaag tgcaaatttg cgacgacaac 6540 gagaaaatcg tcttagggaa gaacaagaac aatcgaaaag aagaaaacag gagactccaa 6600 gcagtaaaat ttcagaattg gatagatcca ttcggatacg atggaagcgg aaatatgctg 6660 atcaagtaaa tgatgcatac ttgagatcta tctactcatc ctttgggact ctacaaaatg 6720 tagttatcca aaaggatata tcgaaagaga aaaaatat 6758 //