ID   SPBPJ694   standard; DNA; FUN; 2097 BP.
XX
AC   AL136498;
XX
SV   AL136498.2
XX
DT   17-JAN-2000 (Rel. 62, Created)
DT   24-APR-2001 (Rel. 67, Last updated, Version 2)
XX
DE   S.pombe chromosome II PCR product pJ694.
XX
KW   decaprenyl diphosphate synthase; dps.
XX
OS   Schizosaccharomyces pombe (fission yeast)
OC   Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes;
OC   Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
XX
RN   [1]
RP   1-2097
RA   McDougall R.C., Rajandream M.A., Barrell B.G., Saunders D., Harris D.;
RT   ;
RL   Submitted (17-JAN-2000) to the EMBL/GenBank/DDBJ databases.
RL   European Schizosaccharomyces genome sequencing project, Sanger Centre, The
RL   Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail:
RL   barrell@sanger.ac.uk
XX
DR   GOA; O43091; O43091.
DR   SWISS-PROT; O43091; DPS_SCHPO.
XX
CC   Notes:
CC   Details of yeast sequencing at the Sanger Centre are available on
CC   the World Wide Web.
CC   (URL, http://www.sanger.ac.uk/Projects/S_pombe/)
CC   During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced
CC   by the Sanger Centre.  The sequencing of the S. pombe genome is now
CC   being continued with funding from The European Commission.
CC   Fourteen European sequencing laboratories, including the Sanger Centre,
CC   are participating in the project.
CC   Protein coding regions (CDS) have been predicted with the help
CC   of computer analysis using the Genefinder program in PomBase
CC   (an ACEDB database) with additional predictions for the
CC   branch-acceptor sites supplied by the program Sp3splice.
CC   CAUTION: It is possible that for any individual CDS we may have
CC   underestimated or overestimated the number of introns/exons or
CC   we may not have chosen the correct splice donor/acceptor sites.
CC   CDS are numbered using the following system eg SPBC25H2.01c.
CC   SP (S. pombe), B (chromosome 2), c25H2 (cosmid name),
CC   .01 (first CDS), c (complementary strand).
CC   The more significant matches with motifs in the PROSITE
CC   database are also included but some of these may be fortuitous.
CC   The length in codons is given for each CDS.
CC   IMPORTANT: This sequence MAY NOT be the entire insert of
CC   the sequenced clone.  It may be shorter because we only
CC   sequence overlapping sections once, or longer, because we
CC   arrange for a small overlap between neighbouring submissions.
CC   PCR product pJ694 is overlapped by cosmid c947, EMBL entry SPBC947,
CC   accession number AL021837, at the 5' end.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2097
FT                   /chromosome="II"
FT                   /db_xref="taxon:4896"
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="972h-"
FT                   /map="IIL"
FT   misc_feature    1..100
FT                   /note="nominal overlap with cosmid SPBC947, EM:AL021837 S.
FT                   pombe chromosome 2"
FT   CDS             join(856..1830,1912..2073)
FT                   /db_xref="GOA:O43091"
FT                   /db_xref="SWISS-PROT:O43091"
FT                   /label=dps
FT                   /note="SPBPJ694.01, len:378"
FT                   /gene="dps"
FT                   /gene="SPBPJ694.01"
FT                   /product="decaprenyl diphosphate synthase"
FT                   /gene="SPBPJ4664.01"
FT                   /protein_id="CAB66154.1"
FT                   /translation="MIQYVYLKHMRKLWSLGKVRSTVLRFSTTNRNASHLIKNELEQIS
FT                   PGIRQMLNSNSEFLEECSKYYTIAQGKQMRPSLVLLMSKATSLCHGIDRSVVGDKYIDD
FT                   DDLRSFSTGQILPSQLRLAQITEMIHIASLLHDDVIDHANVRRGSPSSNVAFGNRRSIL
FT                   AGNFILARASTAMARLRNPQVTELLATVIADLVRGEFLQLKNTMDPSSLEIKQSNFDYY
FT                   IEKSFLKTASLISKSCKASTILGQCSPTVATAAGEYGRCIGTAFQLMDDVLDYTSKDDT
FT                   LGKAAGADLKLGLATAPVLFAWKKYPELGAMIVNRFNHPSDIQRARSLVECTDAIEQTI
FT                   TWAKEYIKKAKDSLLCLPDSPARKALFALADKVITRKK"
FT   misc_feature    join(1030..1100,1179..1866)
FT                   /note="Match to PF00348 polyprenyl_synt, Polyprenyl
FT                   synthetases Score 137.19"
FT   misc_feature    1831..1836
FT                   /note="gtaaga, splice donor sequence"
FT   intron          1831..1911
FT                   /note="intron confirmed by EST"
FT   misc_feature    1896..1911
FT                   /note="ctaacatataatttag, splice branch and acceptor"
XX
SQ   Sequence 2097 BP; 682 A; 355 C; 383 G; 677 T; 0 other;
     aaatatgaat gtaacgtttt gtactacaat gacaataatc ttagcaggcg aatcgttttg        60
     tagactagaa aagtacgtaa tttattttta ttaaatgatc ctacaaatac taaaacttgt       120
     aaaaatgctt ttaagcaatt tctgatcaat taaaatttca cgcatgtcaa accatttaga       180
     aatgtttatg ttgttaaagc agtgttacaa agctattaaa atacctaaaa aatatcaaca       240
     atgtattcgt aaattcctag aaccaataat tgttcaagta agatgagaaa ctcttggaag       300
     atcaacacat atagaggatt ccctgaagct gttatattaa cttcgccaaa aaaaaattag       360
     ttgtagtata agattcattt acttatctaa atattctgct tttgagtatt caaccaagta       420
     gcacttctcc catcgaatat tgaagcaatt tggaagtata aattgttttg attattgtgt       480
     cgtataaaaa atgatacacg ggagagatgc atatgcaaat agatctgtga attttattgt       540
     taactgattt gatgtataag ctgttgtaag ttttgataat gaatttggcc tctgaggaag       600
     atgctcaata tcctgcttga ctcaacagaa ttcttcagaa aaaatactta agatcagaat       660
     ggtatgaaga aaaaacgata attaaaaaat gttttgtttg tttacaaact atttaatgat       720
     tcttccgttc tttgaaataa acttatctga tcaaagcgac gtcatatcta agcgaaatca       780
     cggtaaatac catgatatac aaaccatccc aactctaact tcatcattta gttacttttg       840
     aaaataagaa atgaaatgat tcagtatgta tatttaaaac atatgaggaa attatggagt       900
     cttggaaaag tccgttcgac tgttcttcgg ttttctacta cgaaccgcaa tgcttcacat       960
     ttaattaaaa acgagttgga acaaatctca ccagggattc gtcaaatgct gaattcaaat      1020
     tcagaatttc ttgaagagtg ttctaaatat tataccattg ctcaaggaaa acaaatgcgt      1080
     ccttctcttg ttttgctgat gtccaaagct acaagcttgt gccatggtat tgatcggtcc      1140
     gtagtgggcg acaaatatat tgatgatgat gatttaagat cattttcgac gggtcaaatt      1200
     cttccttctc aattgagatt agcacaaata accgagatga tccatatagc aagtttgctg      1260
     catgacgatg tgattgatca cgctaatgtc cgtagaggct caccttcaag caatgttgct      1320
     ttcggtaatc gacggtcaat ccttgcgggt aatttcatcc ttgcacgggc ttcgactgct      1380
     atggcccgcc ttcgaaatcc ccaagttacg gagttgttag ctacagtgat agcagacttg      1440
     gttcgaggtg agtttttgca gctaaaaaat actatggatc cttcatcttt ggaaataaaa      1500
     caatcaaatt ttgactatta tattgaaaaa agttttttga aaacagccag tttaatttcc      1560
     aaaagctgca aggcttctac aatcctcgga caatgttctc ctactgtagc aacagctgct      1620
     ggagaatacg gtcgatgcat tggtactgct tttcaactaa tggatgacgt gttggactat      1680
     acgtcgaaag atgatacttt aggaaaggcg gctggtgcag atttgaagct agggttggct      1740
     acagctcccg tcctctttgc atggaaaaag tatccagaac ttggtgcaat gattgtgaat      1800
     agattcaatc atccttctga tatccaacgg gtaagagaag ctgaagtttt atttatttat      1860
     ttgatttcct tttctctatc gttaattttc aattgctaac atataattta ggctcgttct      1920
     ttggttgagt gcactgatgc tatcgagcaa accatcactt gggcaaaaga atatatcaaa      1980
     aaagccaaag attcccttct gtgtctccct gattcacctg caaggaaggc actttttgcg      2040
     ttggctgata aagtaataac gagaaagaag tgacgagttt agtacgctgc atcttta         2097
//