ID SPBPJ5566 standard; DNA; FUN; 8067 BP. XX AC AL596271; XX SV AL596271.1 XX DT 25-JUL-2001 (Rel. 68, Created) DT 25-JUL-2001 (Rel. 68, Last updated, Version 1) XX DE S.pombe chromosome II PCR product pJ5566. XX KW CEN2. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-8067 RA Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (17-JUL-2001) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC PCR product pJ5566 is overlapped at the 5' end by BAC pB36C4 CC and at the 3' end by cosmid c633, EMBL entry SPBC633, CC accession number AL391715. XX FH Key Location/Qualifiers FH FT source 1..8067 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid pJ5566" FT /map="IICEN" FT misc_feature 1..433 FT /note="nominal overlap with pB36C4 S. pombe chromosome 2" FT misc_feature 1..4136 FT /note="imr2r" FT misc_feature 4137..8067 FT /note="cnt2" FT misc_feature 7845..8067 FT /note="nominal overlap with SPBC633 S. pombe chromosome 2" XX SQ Sequence 8067 BP; 2887 A; 1173 C; 1116 G; 2891 T; 0 other; ttcttgcagg cattcacaag ttttttgtaa tcctggtcga tgtgctgtcg tcgagagcgt 60 taccatcgct gctatataca tcagtttaat tagcaacact tttgatggct acactgattt 120 tatagattaa aaaaaactgt aataaatcac caaacctttt aaaaattact tgcccattct 180 ttcaaaatac aaaatacaaa attgacttga agagcctatg atctgatttt ttcaacttgt 240 acgacctcta ccttgtcatc tttcttttga tactttatac ataatggcaa acagaatgca 300 caaatgaatg cggaggttca attccttctt tttctctctc acacttaaca ctcagtattt 360 tagtaaatca tgaagacaaa tctttgatga atgcctgttc aaactcttta gtagtgtata 420 tagtcacagg atccattaac tcctatcaca aaatgagtcg tcgcaaattg gaataataat 480 gatattgtgg tttttttagt ccagagactt gaatacagcc tacaattttg gattctctga 540 aggagatcgt taatttatta tgaccaaacc cagaataaaa ctctctatgc attaacaaac 600 tagcaaataa atgcttaatt aaacacactt acatatccgt gtatatgtgt tacaataatc 660 aaaggatgat catcgactac aaagatataa tataaacctg cttttttatt tctattacgt 720 aattttccaa gtttattaat tcaacgactg aattgtgttc atttatacat cacatggtcg 780 ataatttccg ttgtaaataa tgtttgtgcg taaaaataaa tgagtgagca agttaaatat 840 tttttaaacg aaagcaaaac tggatattaa tctaaacaat tagtgccaag tgagagcgta 900 atatgccttt gtgctcacaa atcagataat catgtcttta tcaaataaac tgagtaacaa 960 ttacgaaatt aagaagaccg tgtctcacat gatagtacta ataaaaatat actttgacat 1020 ctgaaagaaa aatgacatat taagagatcg gcaaacgaga acaatgcttg tgtctaatct 1080 ttaaaaccat tgtaaaccaa atatgcattt ggttattgga ttgcgattgg ggaagatgaa 1140 atatttaaac ttgctttact gctttacgta aagttataga caccattgtt gataggtggt 1200 acgaaagaaa agtttatcca tagcgaagag tagtatagtt tgtaagtcat ataataatgg 1260 catcaatgaa gtcacggttg tgaatttact aagaaaatgc agtaaacctg caaacgtatg 1320 ttacgtaagg aattccataa atacttaata cgttttcgtt actaagctga tccaaaaatt 1380 gcaataaatt accgagagca ttcacttaat aattggaaag taatagggaa tagtaataac 1440 ctagaagtac taaaaatgtt acttttaaag aaatattagt taaggtagta gaatatgcat 1500 aaaagtaatg gcaaaattgt aggaattaaa taatgattat tgcgaaaaaa taaatatttt 1560 attaatacat ctttgtgaca tcagaagtta ttataattta ttttactcgt gccatcaata 1620 atggtcgcat gatgtagtcg gttatcatgt cgatctaata agtcgaatgt cgccggttcg 1680 aacccggctg tgaccatgtt ttttattttt taatgaatct cacagtacac tatacatcag 1740 taaatttcaa aaaaaaaata aacaaaaagt ggacaagcca gaactcgaat ctgggacctc 1800 ttccatgcta aggaagcgtg ataaccatct acaccacatg cccatggttg atttttactc 1860 tatgttagtc gattattact ttctttgcta tttgctatta ttcttttcca acgcactttg 1920 atactaccca cgcttatcat agtatacaaa tataacatac atacaaatac aacatacata 1980 catatatgta tatatatata tatttactac cactgcaaat aattgttaca cctttactac 2040 tgttgagttt tcctccgttt ttttatttgt tttttctatt gttttaaatt aattattaac 2100 tatctttctt tttagtccaa atactcaaaa atacttcaaa ctagcttcat tagtaacatt 2160 aactaactaa attttttatt cagtttttgt ttgtatattt ttcagcactt aatatttctt 2220 aagataatta tttttacagt ttccctcttc tttttttttt tttatttaaa tccgatgcga 2280 aaataattaa ttacaaacaa cagataatta gtaattacac ttttgaaaaa aatgagaact 2340 acaatggaac aagtaaataa aaaaaaaaat ttaatccaac caaaattgct gatctcattt 2400 tatttaaaaa agtaaaaatg atttcattcc aaaagaatcg ttaattaatg aagcagatat 2460 aatttctttt ttaaaagtaa aaaattaaaa acttaaaaac gattgatcgt gccaagactc 2520 gaacttggga ccgtctgcga actgtaaatg ttaagcagaa attataacca tctaaaccac 2580 acgaccagat gttggtttta ttattagctt tatttgtaat ataggtttat aaatgaacct 2640 taacgtcaaa ttgtctgaag ttacgaatac acaaattatc tttatctgca tgtttcaact 2700 atcttagcta cattatctca tgcatagaac ttttttgaaa tttatcaata tggctttact 2760 atcagaaatt cttcaataac aatcttcatt cttaacttac gtatatcgta acaagcttgt 2820 aagcataata tgttcagaac tctgtccttt ctaatggaaa ataatactta tttaagcaat 2880 tttatcagct acgaacgacc actactacga tgtatgcatg tgaataatat ttacattttt 2940 tattcctatg tctactgttt aaactaagta ttgtaaatac ttataaaatt ttattatgat 3000 ataatgagct tgttctttat tttgcaaagc aatatggctt gcatataata cataggctac 3060 aattcaatgt acattcaagt attgaaaagc ttttcctgtc tttccaattt aaaaacactc 3120 aacttcggcg acgtgatata agtataggta taataaataa agcgtttttt ataaccagtt 3180 ccgcaaatgt aggaagttta atcaatttta aatgttgaaa agttataaga aatagtgatc 3240 caattaatca tgccatggaa taatttttat aaaccggtaa tcgttgcaaa gtgcttaccg 3300 tttactttta gggcgaaaca ataatacaat taggtagtac cagatcgttt atgaaactgc 3360 ttttagatag gtactttaaa accacatgag gtttcagtga acaacgtttt gttatttttt 3420 aagtaatgaa cttaaacttt ctttgtttac tggttcttat cttactatca taagtattag 3480 taatgtaatt ttcggtcaaa aaaggtgcat aaaacgacaa aaatgtgttt taaaatttcc 3540 atttaattta ctcatctcat caattttgta aagctaacga ggtatttatt ttttgcttgt 3600 ttttattttt aactagttta cgttaaaatt tcacttattt agttacgatg aatacttggg 3660 ttaatgtaaa aatggaatga ttcgaaacaa aattagtttt atcacattcc tgttttcgta 3720 ctttgccttt tactatataa ataaaaaaaa aaaaaaaaaa aaaaggaaat tgtaacttga 3780 aatttatgtg attaatttaa gcattagcgc cttcaataat ttatgagtaa gatggtaaac 3840 aggggtttga tttgattata aagggtataa acggctatca taacttttgt taatccaggc 3900 acttttttat tgtattctga tttaagagct ttgatatgat gcatttgaac attttcggta 3960 gatttacaaa tacaaaacat tcttttaatt ttgatttggt tctaattgac caatatactt 4020 cttctcatgg aaacattagc gttcatatta aattttatca aaaatcatca gttcgaaatc 4080 attctacttg tatcacttca aacaaatgcc aaagttttta acttaacaaa acaaaacgaa 4140 attttttttt tgttcttacg tttttcaatt actgggggga caaattacgt actaactgtt 4200 agtttctatg gtgatttcgt tttaattagt tactttttta tttattacat tcaaaaacac 4260 agcttttgct gaacaagact tgcgtatgaa cgcgctaata caaatataaa aggggttaat 4320 tttcttctta attatttaca cagaagcgag cactgtttac atctaacgat aatcagaaaa 4380 ttgtgttggc aaaccctaga atgactttaa ttttttagta atatgttctt ttattgttta 4440 gcaaacgtat acttacaaaa ggtaattttt attagatttt ttaaaataga aatgatcttt 4500 ttgcttaaaa gtttaatggt aatattgaga tggcccatga ctataagatg ctattaagta 4560 acgtttatct gaaaatatca ttatcattgt tcgtatcgca atttgagatt tactaaacct 4620 ttttttaaaa tgaaaacgcg aactccgata atcattagta cgaaaatcaa aaaaaaaaag 4680 ttgaagggta cgtttaattc ttggttttaa aattcgacga gtttttagca tcaaagcaaa 4740 aacctgaaga tttgtatctt attcattttt ctttaagcgg tttagctggt taaagaaaaa 4800 tgttttccaa taatatttat tcacatgtac tgctttgtga tgcgtaagcc gttactcgtt 4860 aatatgaaga agcattggtt agtcctggtt gatttctatg tttccttttt ggcttcggcc 4920 aattctactt ttttaagtat tccgtgtgtt atctttatgg actattattt taaaatctgc 4980 atattaggtt taagtatgaa ttagttaggg atacgatttt ctaaatttac atactaataa 5040 cggaatagat caaacaagct ccttcaattt ttagaaaaaa aaaaataacg cattaattgt 5100 aaatgtttta ggaaattatt ggtcaaatct atgaatttgc tggttaaata gtatatcagg 5160 gggttttatg attttggcga agtgtcttat ccttcatgtt aaatgtatta attttctttt 5220 ttcacaaata tcctgtcgat taaaaaatta aaagtaacac atatgtagtg gaatttatct 5280 aaaacctcag aatttttacc gagttgtaaa cttgacgaat gacatgaaat ggtaaaagtg 5340 agcaagctca taaaataaat ccacgcttgt cgcaaaacat aaagcaaatt tgaaaaaaag 5400 aagggtaaat atattaagat atgatttatt taaaagcaaa tcatttaact tacaattgcc 5460 taatgtgata actatatcaa aaatgcccct agcaaagtag gcaacttggt tttattgatt 5520 aacaaaatta tgtataagag attaaattat cctgtttaga aacttactat ttctattaca 5580 cttgctagat agctaagtat taatttatac aaagccataa tgttgctaac gaaaccttat 5640 tattgcacat tttgacttga aagggagtct gtaaaccagc tataatttaa ataagactat 5700 agcaaatatt ttagtttata agaaaaatga atctattaac aacacccgtc atttatgaaa 5760 attaacaaca taaatcaaat atgctatatg ttaaaattag tactagcgtt aaggtaattc 5820 gcataagtga actaagagtc aaagttattt tgaacatagt taataaaaca acgatctaat 5880 ataaattaat taacgttagc aaggagtttt gttccgttca ggtttactgc taacttcaaa 5940 tcaagaatca ggccattcat taaaaggagc aatacagaat tctaggaaag ttacggttta 6000 ccagcatatt ttgaggtaca tgaaattttc atcaaacata ttgtttatca tggataagca 6060 ctaccactaa tttattaaca tgacaatgcg ctctaatcca tcaatttatc gataaacgta 6120 tgattgcttt caccactgat tttttatttt attttttttt tttactatga tagtgccgac 6180 taggataaac gtgtaaatta tatcgatgca tttttagaac aagtattact aaatatgcat 6240 atcattcaag taaaaaatgt ttgaaaattt taattgcgtt agcatattct atttgtccta 6300 gtaattgcat ctattgtact ctctcatcac gtaaaaaatt atttgactac agatagattg 6360 ctgctgagta acttattcac acgacgtgca tatccatcct tcgttgaaga atgcaaaaac 6420 tcaaattagt taaaattttt accgaatgtc ccccattaaa ttgaatttac ttaattcagc 6480 tttctatttc caacgaaaaa aaatattatt tccaaaatcc aaattaattt attaaataac 6540 atcaatcaaa gacgaaaaaa gccttattct tcgagaaacg aaacaataaa ttacctcata 6600 attcagtttt ctattcctaa gaccataatt cgaataatct ttttattaaa attttttaaa 6660 tttcttctta gtgattttac aacacaaacc aactgttaga gtagtaggag aagggattgc 6720 tgtagaaact gctaggtgtg caaaatttaa aagataaact tactttatag actatatgtt 6780 tataactaaa tactgactag actttaagtt ctaccctatt actggaaaag ttatcgaatt 6840 cctttaacgt accttttgac ctttaagaaa cactaaataa aattgaacgt aaataataag 6900 taacgaattc tctgaacctt tttcgataat tcatgtgctt gaataaacta atatgtgcaa 6960 aaaatcaata ccttctttaa ctcgacttta taaaatttcc cttaagataa taattattag 7020 caatgtttaa tttgaaagca aaaattctgg tattatactt gttaagcgct aactcgttta 7080 agtgaaatac ggacaaaaaa aaagtaatga atgcgcagaa aatatcaaaa tacgggtaaa 7140 ttgatttgtt tgttaattgc cattctttgg cgtattgaga taattagcaa ttgccttttt 7200 ttaatgcgtt catacagatg actttccacg ccatgtcatc aattcttttt ttttatgtag 7260 atacacataa ctcgcatatt aactacatta gaaattttaa aattaacata tcttgtttgt 7320 aatttacaaa ccataaagtt ttatgataat tgttgtgatt atcaacttta ctaatttgac 7380 tcctaatcag ttagaattat tttaataaat ttactaaaac attaaacaaa caacggcaca 7440 ctgttttttt ggtcacagct tctaagcatg caaatgaaat tactcaagga atttgctggc 7500 ttaataattt tatttaataa ataaagatta tagtagaaaa gaatgaaaaa gtatttagtt 7560 gacgatagta tttgttacaa attaaaaact caaaaccatt cttgcagtaa gtcaatcgtg 7620 attgacattt tctaaaatat cacttactga acccatgcaa gatttagcgc agagggaaac 7680 ttttatatct ggtagctgag aaacctagta atgatggtaa taaataaata tcaacattca 7740 gtttaacata gctataaacc cctaactgta accgtagtaa aagctcaaat ggctgtatag 7800 aagaagttga aaatcgttta aagaaacaat gttgtagttt gaaggatcag ttggtaaaac 7860 aaatctatta tagaaattat attaatttca gagagcctca tattttacgc aaattatgaa 7920 ggtaaatatt gtaatcagac catttgcgaa acagtaaatt tcttttttct gtttggtttt 7980 gcttgtgatt tggttcatgg tttttattaa tattttcaag atagtttcta aagatcatcc 8040 agaacaattt ctattccttc attattt 8067 //