ID SPBP18G5 standard; DNA; FUN; 6100 BP. XX AC AL512563; XX SV AL512563.1 XX DT 11-JAN-2001 (Rel. 66, Created) DT 11-JAN-2001 (Rel. 66, Last updated, Version 1) XX DE S.pombe chromosome II p1 p18G5. XX KW eif-2alpha kinase; Phosphatidylglycerophosphate synthase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6100 RA Collins M., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (05-JAN-2001) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9HDW2; Q9HDW2. DR SPTREMBL; Q9HDW0; Q9HDW0. DR SPTREMBL; Q9HDW1; Q9HDW1. DR SPTREMBL; Q9HDW2; Q9HDW2. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC p1 p18G5 is overlapped at the 5' end by cosmid c36B7, CC EMBL entry SPBC36B7, accession number AL391743, and at the 3' CC end by cosmid c29C10, EMBL entry SPBC29C10, accession number AL358273. XX FH Key Location/Qualifiers FH FT source 1..6100 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="p1 p18G5" FT /map="IIR" FT misc_feature 1..247 FT /note="nominal overlap SPBC36B7 S. pombe chromosome 2" FT CDS 1..2268 FT /codon_start=1 FT /db_xref="GOA:Q9HDW2" FT /db_xref="SPTREMBL:Q9HDW2" FT /label=SPBP18G5.01 FT /note="SPBP18G5.01, len:756, SIMILARITY:Saccharomyces FT cerevisiae, GCN2_YEAST, protein kinase gcn2, (1590 aa), FT fasta scores: opt: 1021, E():0, (31.7% identity in 745 aa)" FT /partial FT /gene="SPBP18G5.01" FT /gene="SPBC36B7.09" FT /product="putative eif-2alpha kinase; possibly involved in FT involved in general amino acid control response by FT similarity to yeast gcn2" FT /protein_id="CAC21489.1" FT /translation="GTALYVAPELLSRRNGVRYDAKVDMYSLGIILFEMCMTFSTSMER FT IRIIDTIRSPSISFPSTFPFSRASHEFKVIHCLLQHDPTKRPSSQELLESEAIPPKVGE FT EFIQEGLRLLSNPNTPYYLKLLKVLFGQVPDRHKDFTYDFNLSEESGVLSKVSDRGWDS FT LLACLVRDHVVKVFRRHGAKERESHILFPKSSQYDKDQASVSLLDKNGTLLQLPYDTVL FT PYARNVARNAVEEEKTYLISDVFREAKGGGRPKAIKEISFDITTNSDNLDWYDAETIKA FT LDEVLTEIPSLTESCILINHADILSSILDYLQVSKDKRRMATHILGQINQRLTLSQVRN FT QLRIESLVPSTTLDDLSLFDFRENYEEGASKLRKIFGKEMPQKMRTALNYMERVVKLLR FT ALKISHQLYFMPLCVYNFEFYDGGLMFQAINLAEKSELICAGGRYDKLVRFFDPPLMRT FT ARKKHVVGICFALEKLVFSMLRYIRFHNSKQSSKHSPSPTLKSVGPWAPRRVDVLVTSI FT GKDSILEKCSLLQELWALNIQADIVLRGASSLEEIVTHYRSEGINWVLVVRQKNTQMEH FT SVKARNILKNEDDEIRFDEVGMWLLGEINERKRNESMLQSKRILDSAQQDVAKFVDTSQ FT SNLDVQLISLKDVNDRKYKWKHKQNAMNKVYDLVQSAIRESSEDAIALAVDCDSEAMEK FT LRSTTTLDEESWKRLIESCPASQREYMQRLQKKLVTLAEQDKKRVWICSFRTNEIYLYG FT LK" FT misc_feature 4..285 FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 45.60" FT CDS join(2567..3051,3089..4208) FT /db_xref="SPTREMBL:Q9HDW1" FT /label=SPBP18G5.02 FT /note="SPBP18G5.02, len:542, SIMILARITY:Neurospora crassa., FT Q9P713, probable pel1, (499 aa), fasta scores: opt: 679, FT E():0, (38.9% identity in 499 aa)" FT /gene="SPBP18G5.02" FT /product="putative Phosphatidylglycerophosphate synthase; FT the first enzyme of the cardiolipin biosynthetic pathway" FT /protein_id="CAC21490.1" FT /translation="MDEGIEKNIFVNLESQIDGVCPKFYVNVDDIDIIHEPPEFYQRLK FT KLIKKAQKRIFLSTLYIGKEERELINCLSNALSNNPSLHVHILADQLRCTRESPGCCSA FT SLLMQLKKKFPDRCEIKLYHTPNLRGLRKQLVPHRFNEGWGLQHMKIYGADDNLIISGA FT NLSRDYFTNRKDRYYLFSDKGLADFFFKTHFLFSQLSFECIPHLSDSSIQLSSTSPVIP FT FTLKWNNSCPNPLTNPQEFRVAASAKIQQLLQGNREKFLSRNPSKPLSSVYGSELINQA FT GDDNNKPFHKYEESAIVYPLFQCVPILTSDVHSTEEKVLSIIGTLLSRKEVNWTLTAGY FT FNVYPALRKQLLKSEGIGEVIVASQQANGFYRSPGPSKLIPPAYQYIAEQFLKDSRKKK FT RNIDVLQWQNKGNTYHAKGKHYSLSFSIINIFWSTALKSYIFEKFTNTILGFWLSTQHH FT KHPFLTTIGSSNYTSRSQQLDLESTLVVMTQNEKLKRKFSTEIELIKQHTKPMNTCQLE FT KVPMYVKALTSLMKKKL" FT misc_feature 3052..3057 FT /note="gtaaag, splice donor sequence" FT misc_feature 3078..3088 FT /note="ctaacgaatag, splice branch and acceptor" FT CDS join(4395..4517,4560..5729) FT /db_xref="SPTREMBL:Q9HDW0" FT /label=SPBP18G5.03 FT /note="SPBP18G5.03, len:431, SIMILARITY:Schizosaccharomyces FT pombe, O94294, leucine-rich repeat protein - weak FT similarity to adenylate cyclase., (886 aa), fasta scores: FT opt: 147, E():0.25, (20.0% identity in 410 aa)" FT /gene="SPBP18G5.03" FT /product="hypothetical protein" FT /protein_id="CAC21491.1" FT /translation="MQLQSPFGDGISCECLNIRVLGIPNETKHQWIFVPRDLIKIKIYS FT LLQICKAENCSAVACRGCNLCILAVQGNIEISEEPQKLFQEENVKVYIYDSAISLSSVR FT ASFPISELGIRMDIIKARAEPREDEIRASFSSLVNKEIKRTVEELLLSKRSTNRLSLLA FT FMRNQQLNYEAYETKLLEDASTIEKSLEKEVKTIYDSNIASPKESSDAIDADHAMIDES FT RSTQRRKSKPKKHVAFTDEYQVAFSDKPGKYFNQPMQYSKQFKLDNPDYEASSDSLNDI FT ENLSTLTFRSDEELEFDFDTNNINNNKSGDSLEMSTTIPSDEENEDFTSKVDAMEIHSG FT SLPLNIDSSPIFTNHSPSSSLSSESSFEASPSSFVDRKNRWIQMLKKADEHSRSIQARS FT MGYVLSDDLDNSKAFRPYKQSFLAQGWKSLN" FT misc_feature 4518..4523 FT /note="gtacgc, splice donor sequence" FT misc_feature 4548..4559 FT /note="ctaattcaatag, splice branch and acceptor" FT misc_feature 5902..6100 FT /note="nominal overlap with SPBC29C10 S. pombe chromosome FT 2" XX SQ Sequence 6100 BP; 1986 A; 1089 C; 1129 G; 1896 T; 0 other; ggtacagctt tgtacgtagc tcctgaactt ctcagtaggc gtaatggggt acgctacgat 60 gcaaaggttg atatgtatag cttgggtatt atactcttcg aaatgtgcat gactttttct 120 acttctatgg aaagaattcg gataattgat actattagat ctccatctat atcgttccct 180 tccacttttc ctttttcccg tgctagccat gagttcaaag tcattcattg tcttctacaa 240 cacgatccca caaaaagacc atcgagccaa gaactccttg aaagtgaagc catacctcca 300 aaagttggtg aagagtttat tcaagaaggc cttcgtcttt tgtccaaccc taatactcct 360 tattatttaa agcttctgaa agttttattc ggccaagtgc ccgatcgtca taaagacttt 420 acttacgact tcaaccttag tgaagaaagt ggagtgttgt cgaaagtgtc ggatagagga 480 tgggatagtt tgttagcttg tttagtacgt gatcatgtag ttaaagtatt tcgtcgccat 540 ggagccaagg aacgtgagtc acatattcta ttcccaaagt cttctcaata cgataaagac 600 caagcttctg tttccctact agataaaaat ggtacccttt tacagcttcc atatgatacg 660 gttcttcctt acgcgagaaa tgttgctaga aacgctgtgg aagaagaaaa gacgtactta 720 atttctgacg tttttcggga agccaaaggt ggagggagac caaaggctat aaaggaaatt 780 tcatttgaca ttaccacgaa ttctgataat ttggattggt atgatgcaga aacaatcaaa 840 gcattagacg aagtattaac agagatacca tccttgacag aaagttgtat cttaattaat 900 cacgctgaca ttctttcctc cattcttgat tatttacaag tatcaaagga taaaagacgc 960 atggctactc atatacttgg acaaatcaac caacgcttaa cattatcaca ggtacggaac 1020 cagcttcgta ttgagtcatt agttccctct accaccttag acgatctgag tttgtttgat 1080 tttcgggaaa attatgaaga aggtgctagc aaacttagaa aaatttttgg gaaggaaatg 1140 ccacagaaaa tgagaactgc tttaaattat atggagcgag tggttaaatt acttcgagca 1200 ctaaaaattt ctcatcagtt atacttcatg cctttatgtg tttataactt tgagttttac 1260 gatggaggtt tgatgtttca agcaattaac ttggcggaaa aatctgaact catttgtgct 1320 ggtggtcgtt atgacaagct tgtacgcttt ttcgatcctc ctctaatgag aactgccaga 1380 aaaaagcatg ttgttggaat atgttttgcc cttgaaaaac tagtattttc aatgctgcga 1440 tatattcgat ttcacaatag taagcagtca agcaagcatt ctccttcacc aactttaaag 1500 tctgtaggcc cttgggcacc aaggagagtt gatgtcttag ttactagtat tggcaaggat 1560 tcaatcctag aaaaatgctc acttttgcaa gagctttggg ctttaaatat ccaagcggat 1620 atagttttaa ggggtgctag cagtttagaa gagattgtaa cacactatcg aagtgaaggc 1680 attaattggg ttctcgtcgt gcgtcagaaa aatacccaga tggaacattc agtcaaggct 1740 cgaaatattt taaaaaatga agatgacgaa atacgctttg atgaagttgg gatgtggttg 1800 ctgggagaaa ttaatgaaag aaaaagaaac gaaagcatgt tacagtcgaa acgtatatta 1860 gactcagctc aacaagatgt tgcaaagttc gttgacacct ctcaatccaa cctcgacgta 1920 cagttgatct cgcttaaaga cgttaatgat agaaagtata aatggaaaca caaacaaaat 1980 gctatgaata aagtttatga tctagtgcag tcagccattc gtgaaagctc agaagatgct 2040 attgctttag cagtggattg cgactcagaa gctatggaaa aacttcgtag taccactacg 2100 cttgatgagg agtcttggaa acgtttaata gaaagttgcc cagcttctca aagggaatat 2160 atgcagagac tgcaaaagaa acttgtcacc cttgcagaac aagataagaa gcgggtttgg 2220 atatgctcat tcaggacaaa cgaaatttat ctttatggac ttaaataaat tttgaactaa 2280 ttaatcattt aattttcatt actcttttaa ttaaatagaa aaaattttgc tataaattac 2340 tttaaatgtg tatatttcgt gatcggtata ttattttttg ttaagtaagt tagagtcaat 2400 ttgatacttt ttatttttaa tataacatgg aagttctgct tttataccat ttttctctat 2460 atttcacgtt caaattaccg ttggatattg acctttctac tgcatgaacg ggtttgcaag 2520 ctcaatgtat ctcacactaa cctagcacac caatttaaac agcctaatgg atgaaggaat 2580 agagaaaaac atatttgtca acttggaatc tcagattgac ggtgtgtgtc ccaaattcta 2640 tgttaacgtt gatgatattg atatcattca tgaacctcct gagttttatc aacgcttgaa 2700 aaaattaatc aaaaaagctc aaaaaagaat atttctatca acgctataca ttggaaaaga 2760 agagcgagaa ttgatcaatt gtctatcaaa tgcgctaagc aacaatccct ctttgcatgt 2820 tcacattctg gcagatcaat tgcggtgtac tagggaaagc cctggttgtt gctctgcatc 2880 tctattaatg caattgaaaa agaagtttcc tgatagatgc gaaatcaagc tttaccatac 2940 accgaatttg cgtggactaa gaaagcaatt ggttccacac agatttaatg agggatgggg 3000 attgcagcat atgaaaatat atggtgcaga tgataacctc attatcagcg ggtaaaggca 3060 aatgattatt tactatacta acgaatagag ccaatctttc gagagattac tttacaaatc 3120 gaaaagaccg gtattatttg tttagtgata agggtttagc ggactttttc tttaaaacgc 3180 attttttgtt ttcacagctt tcttttgagt gtatccctca tttatccgat tcctcaattc 3240 aactatcttc tacgtccccg gttattcctt ttactttgaa atggaacaat tcatgtccaa 3300 atcctttaac aaatcctcaa gagtttcggg tagcagcttc tgctaaaatt caacaacttc 3360 ttcaggggaa tcgagagaaa ttcttgtctc gcaacccttc taaaccgtta tcatcagtat 3420 atggctctga attaattaat caagcaggtg atgacaacaa taagccattt cataaatatg 3480 aagaatctgc tatcgtatac ccactttttc aatgtgtacc tatccttact tctgacgtac 3540 attctactga agaaaaggtc ttgtctataa tcgggacctt gctatcgcgc aaggaggtta 3600 attggacctt aacggctggc tattttaatg tttatccagc acttagaaaa cagttgttga 3660 aatctgaagg tataggggaa gtaatcgtgg cttctcaaca ggctaatgga ttttatcgat 3720 ctcctggacc atcgaaatta attccaccag cgtatcagta tattgctgaa caatttctta 3780 aagattcaag aaagaagaaa agaaatattg atgtcttaca atggcaaaat aaggggaata 3840 catatcatgc taaaggtaaa cactacagtc tgtcgttttc cattataaat attttttgga 3900 gcacggcact taagtcatat atctttgaaa aattcactaa cactatttta ggattttggt 3960 tgagtacaca acatcacaag catccttttt taacgacgat tgggtcttcc aattatacga 4020 gtaggtctca acagttggac cttgaatcca ccttagttgt aatgacacag aatgaaaaat 4080 taaagagaaa attttctact gaaattgaac ttattaaaca gcacacaaag cctatgaata 4140 cctgtcagtt agaaaaggtt cctatgtatg tgaaggcttt gacttctctc atgaagaaaa 4200 aattataata tgaatttaat aaaatttata aataaattat caagaattag atttttcggc 4260 gttattaagc atgtagtata gcacaatact gtatcaatta tattacatcg acaacggcgt 4320 acctaattct tgccaacgcc gatctcactg agcaacgcga ttcgagcgtt gacgaaatct 4380 tcatagtaga tttaatgcaa ttgcaatcgc catttggcga tggaataagt tgtgaatgtc 4440 tcaatatacg tgttttagga attcctaatg aaaccaaaca tcaatggata tttgttcctc 4500 gtgatctcat aaaaattgta cgctaacctt aatgaaatca aatataacta attcaataga 4560 aaatttatag tttattgcaa atatgcaaag ctgaaaattg ttcagctgtt gcttgtcgag 4620 gctgcaactt atgtattctt gctgtgcagg gaaatattga aatatccgag gaaccgcaga 4680 aattatttca agaagaaaac gtgaaagttt acatatacga ttcagctatt tctctttctt 4740 ctgtaagagc atcttttcct atttcagaat tgggcattcg aatggacata attaaagcgc 4800 gagctgaacc tcgggaagat gaaatacggg cttcattttc ttcactagta aacaaggaaa 4860 ttaagcgtac agtcgaagaa cttttgttgt caaagagatc aacaaatcgt ttatcactac 4920 ttgcgtttat gcgtaatcag caactcaact atgaagccta cgaaactaaa ttgttagaag 4980 atgcttcgac aattgagaaa tcactagaga aggaagtgaa aaccatatat gatagcaata 5040 tcgcatcacc aaaggagtcg agtgatgcca ttgatgctga ccatgccatg atcgacgaaa 5100 gtcggtctac tcaacgtcga aaatcgaagc caaaaaagca tgttgctttt actgatgaat 5160 atcaagttgc cttttcggac aagcccggca agtattttaa ccagccaatg cagtattcca 5220 aacagttcaa acttgacaac ccagactatg aagcgtcttc ggattcgtta aacgatatag 5280 aaaatctatc tactttaact ttcagatctg atgaagaact tgaattcgat tttgacacaa 5340 acaacataaa taataacaaa tcgggcgatt cacttgaaat gtcaaccacg attccgagcg 5400 acgaggaaaa tgaagatttt actagcaaag tggatgctat ggaaattcac tcaggaagtt 5460 taccattaaa tattgattct tctcctatat tcacaaatca ttctccttcg tcaagtctta 5520 gctcagaatc ctcctttgaa gcctcaccct cttcgtttgt cgatcggaaa aatagatgga 5580 tccaaatgtt gaaaaaggca gacgaacata gtcgaagtat ccaagcccga agtatgggct 5640 atgtattaag tgatgatctt gacaattcaa aagcttttag accttataaa cagtcgtttt 5700 tggctcaagg ttggaagagt ttaaattgaa tagcgtctat gtgttttatc tttacaattt 5760 aggactattt cttgcgctcc tttttttctc acaaagaatg cttgatgatt ttgtttcgtt 5820 gataataatt ttgctattat tttatactat ttttaaataa ttatagttgg ggttaataat 5880 tgatttgctt ttgatttgta tgatcgacag ccgattataa acatgctcga cagtattcga 5940 tcgaactacc tgcataaaca aggtagtatt cagtactaaa ggttagacgc caaactagtg 6000 aatttacaat acctaagtaa ttgatgcagt gttgcccatc aacaccctaa gaacagacga 6060 ctgaaatgat acttcgtgaa acgattctca aaagttacct 6100 //