ID SPBC685 standard; DNA; FUN; 13460 BP. XX AC AL049474; XX SV AL049474.1 XX DT 23-MAR-1999 (Rel. 59, Created) DT 23-MAR-1999 (Rel. 59, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c685. XX KW 40s ribosomal; clathrin coat assembly; glycosylphosphatidylinositol anchor; KW lipid metabolism; orc2; origin recognition complex; orp2; rpl27a; KW sphingomyelinase family. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-13460 RA Seeger K., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (18-MAR-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O14388; O14388. DR GOA; O74369; O74369. DR GOA; Q09142; Q09142. DR GOA; Q9Y7L6; Q9Y7L6. DR GOA; Q9Y7L8; Q9Y7L8. DR SPTREMBL; Q9UST0; Q9UST0. DR SPTREMBL; Q9Y7L4; Q9Y7L4. DR SPTREMBL; Q9Y7L5; Q9Y7L5. DR SPTREMBL; Q9Y7L6; Q9Y7L6. DR SPTREMBL; Q9Y7L7; Q9Y7L7. DR SPTREMBL; Q9Y7L9; Q9Y7L9. DR SWISS-PROT; O14388; R27A_SCHPO. DR SWISS-PROT; O74369; NSMA_SCHPO. DR SWISS-PROT; Q09142; ORC2_SCHPO. DR SWISS-PROT; Q9Y7L8; RS0A_SCHPO. XX CC Notes: XX CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. XX CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. XX CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). XX CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. XX CC The length in codons is given for each CDS. XX CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. XX CC Cosmid c685 is overlapped at the 3' end by cosmid c32F12, CC EMBL id SPBC32F12, EMBL accession number AL023796. XX FH Key Location/Qualifiers FH FT source 1..13460 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c685" FT /map="IIR" FT CDS 1..447 FT /codon_start=1 FT /db_xref="SPTREMBL:Q9UST0" FT /label=SPBC685.01 FT /note="SPBC685.01, len:148, SIMILARITY: Saccharomyces FT cerevisiae, YB06_YEAST, hypothetical 79.2 kd protein in FT rpb5-cdc28 intergenic region., (698 aa), fasta scores: opt: FT 195, E():3.9e-05, (45.3% identity in 86 aa)" FT /partial FT /gene="SPBC685.01" FT /product="hypothetical protein" FT /protein_id="CAB39358.1" FT /translation="IEKASMHAPAKGRNSSMQEPSSKSPLLKTPKSNYFPGYGSLSPNT FT SVELPEINSDYSDDSDDEGNKKKVNLPSWAESPELREQLKRQQKWDPDKIFGMIKPLQM FT DEYFRSKDRSKIRFRPRSSSADWSSQDRLTQAEIDNYKKNMGFL" FT CDS join(961..1123,1179..1243,1394..2170,2300..2524) FT /db_xref="SPTREMBL:Q9Y7L4" FT /label=SPBC685.02 FT /note="SPBC685.02, len:409, SIMILARITY:Archaeoglobus FT fulgidus., O28107, hypothetical 32.1 kd protein., (273 aa), FT fasta scores: opt: 121, E():0.38, (23.1% identity in 342 FT aa)" FT /partial FT /gene="SPBC685.02" FT /product="hypothetical protein" FT /protein_id="CAB39359.1" FT /translation="MEEYEDFEMEDLSELVSYMDYLETQRLTISQLDYSLSIPPQLLVS FT REISKLEDEVCEMLKESSLFQLFRKHKGYLNVTDLVLPLWCEVQHEYYLLRRIKKKTPK FT MERGIKLHQILEYETSPPSERRVLDRTSKEEPWALRLLRQLEGIMLLQKNGITREFPIW FT GYYKESSIFGIIDEISLNNPSKSNFNSDIRNYFNFKMYDLSFVDNKTRFSSRKPGASQI FT LSSKVQLMYYVHLFLNYFPSLGEKQQSIFRDISPTYSNRLHSQSHWWNMFLSQLSLDGT FT KDLGPKFLEQSILSIPDIPEDVFAGHNSLNGLYALVFASAKKLHLRLTDDNLTIAYRND FT KTGEVVYKDKFSFSNKLLEASYTKAYQFWHNLREPEGVPAEEVYKCRSCEFQKECWWLK FT KKQYYPLSSP" FT misc_feature 1124..1129 FT /note="gtacga, splice donor sequence" FT misc_feature 1161..1178 FT /note="ctaataatcaacgttcag, splice branch and acceptor" FT misc_feature 1244..1249 FT /note="gtaagg, splice donor sequence" FT misc_feature 1374..1393 FT /note="ctgacagaacctattattag, splice branch and acceptor" FT misc_feature 2171..2176 FT /note="gtaagt, splice donor sequence" FT misc_feature 2283..2299 FT /note="ctaacatcttttactag, splice branch and acceptor" FT CDS 2774..4132 FT /db_xref="SPTREMBL:Q9Y7L5" FT /label=SPBC685.03 FT /note="SPBC685.03, len:452" FT /gene="SPBC685.03" FT /product="hypothetical protein" FT /protein_id="CAB39360.1" FT /translation="MQFFGSLFVSLLGAAGLANALPSPGIPSNDKDLAIYPPVDANNTT FT SQDVVIDVVLFNGTEWISSRERFSLVSPGWASIYGPSGQSSVGSVLYSPILDYIDNAQL FT LVLDDVSFGVFSSLANSVTGYASNYTQSSSNSTSTMNSTGSVSGGSVYPTNSTTNSSIS FT WNSSTSAATNTSSSSSSSSQSSVVSVNSEIFSYFGLSQQYVNYSTSRLCVVGTPRANMS FT TVSVTNNGSAVSNYTVNTNGWTSSNFKCVDDVVANIFGLDFYTAAVLSEVSILRSFALC FT NATTSSSLFRQIASYGVYGSFHFSSSESGSFANLIGTNNYFMTDVKSSSVVIVQSETSC FT SINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSNSTNSSIPTTYPSNSTTYQNITTSY FT PWSQPVVNITDYLSDNGDGHFVLAGDGNQTIGDFYVMNWTTIASGEYLVPFNY" FT CDS complement(4562..4993) FT /db_xref="GOA:Q9Y7L6" FT /db_xref="SPTREMBL:Q9Y7L6" FT /label=SPBC685.04c FT /note="SPBC685.04c, len:143, SIMILARITY:Mus musculus, FT AP17_MOUSE, clathrin coat assembly protein ap17, (142 aa), FT fasta scores: opt: 562, E():0, (56.6% identity in 143 aa)" FT /gene="SPBC685.04c" FT /product="clathrin coat assembly protein" FT /protein_id="CAB39361.1" FT /translation="MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQK FT FQANFLEWENSKLVYRRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDL FT IFNFYKVSAILDEIILGGEIGESNKKSVLERIEALEKLE" FT misc_feature complement(4565..4993) FT /note="Match to PF01217 Clat_adaptor_s, Clathrin adaptor FT complex small chain" FT CDS join(5919..6126,6303..6577) FT /db_xref="SPTREMBL:Q9Y7L7" FT /label=SPBC685.05 FT /note="SPBC685.05, len:160, SIMILARITY: H. sapiens, GPI FT ANCHOR GPI-H., fasta scores, opt:175, E(): 9.2e-06, (31.0% FT identity in 155 aa overlap)" FT /gene="SPBC685.05" FT /product="similar to glycosylphosphatidylinositol anchor" FT /protein_id="CAB39362.1" FT /translation="MLTIKRYPGAIEFTVHTSKSYGTQMFALCFISFVIGATSLAIGRS FT PKIIITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIR FT DIFINEGFRKFDVCYYMGIAIESETEIHVVFPTLLPRHDVLQKVYKETVILLANNS" FT misc_feature 6127..6132 FT /note="gtatgt, splice donor sequence" FT misc_feature 6284..6302 FT /note="ctaacttacattttactag, splice branch and acceptor" FT CDS join(7000..7038,7094..7933) FT /db_xref="GOA:Q9Y7L8" FT /db_xref="SWISS-PROT:Q9Y7L8" FT /label=SPBC685.06 FT /note="SPBC685.06, len:292, SIMILARITY:Neurospora crassa., FT RS0_NEUCR, 40s ribosomal protein s0, (293 aa), fasta FT scores: opt: 1130, E():0, (60.9% identity in 299 aa)" FT /gene="SPBC685.06" FT /product="40s ribosomal protein s0" FT /protein_id="CAB39363.1" FT /translation="MAQVGRPNILNATDEDIKQLLAANCHIGSKNLEVRMDNYVWKRRS FT DGVHILNLGKTWEKLVLAARVIATIENPADVCVVSTRTYGHRAVLKFAAHTGATAIAGR FT FTPGNFTNYITRTYREPRLIVVTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDIAI FT PTNNKGRKSIGLIWYLLAREVLRVRGTLSRSAPWDVMPDLYFYRDPEEVEREEEAKKAA FT AAAAEEAQVEEAVAAAEFEITDSAAGSVDPNVLAAATAGQVGENTWEGAGDWNTTGAAQ FT TSDWTAAAEAQ" FT misc_feature 7039..7044 FT /note="gtaagt, splice donor sequence" FT misc_feature 7078..7093 FT /note="ctgacactgaatttag, splice branch and acceptor" FT misc_feature 7112..7618 FT /note="Match to PF00318 Ribosomal_S2, Ribosomal protein S2" FT CDS join(complement(8704..8730),complement(8276..8659)) FT /db_xref="GOA:O14388" FT /db_xref="SWISS-PROT:O14388" FT /label=rpl27a FT /note="SPBC685.07c, len:136" FT /gene="rpl27a" FT /gene="SPBC685.07c" FT /product="60s ribosomal protein l27-a." FT /protein_id="CAB39364.1" FT /translation="MVKILKPGKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAG FT VERYPLKVTKSMGAKRIARRSRVKPFIKVVNYNHLMPTRYALELDNLKGLITADTFKEP FT TQRSAARKTVKKTFEEKYQSGKSAWFFTPLRF" FT misc_feature complement(8660..8676) FT /note="ctaacatttcaaaatag, splice branch and acceptor FT sequence" FT misc_feature complement(8698..8703) FT /note="gtatga splice donor sequence" FT CDS join(9393..9395,9442..9732) FT /db_xref="SPTREMBL:Q9Y7L9" FT /label=SPBC685.08 FT /note="SPBC685.08, len:97" FT /gene="SPBC685.08" FT /product="hypothetical protein" FT /protein_id="CAB39365.1" FT /translation="MVLVKFKREKLTVMLEVQPGLSVYDAVKLLKSALNLPPDSILRIG FT SFEQNDWVAMENDALSKTIITNNTEYAFAEGEEPLLVEKPKDYDDMSEEISP" FT misc_feature 9396..9401 FT /note="gtacgt, splice donor sequence" FT misc_feature 9424..9441 FT /note="ttaactaggttcctcaag, splice branch and acceptor" FT CDS 10035..11642 FT /db_xref="GOA:Q09142" FT /db_xref="SWISS-PROT:Q09142" FT /label=orc2 or orp2 FT /note="SPBC685.09, len:535" FT /gene="orc2" FT /gene="orp2" FT /gene="SPBC685.09" FT /product="origin recognition complex protein, subunit 2." FT /protein_id="CAB39366.1" FT /translation="MLINEHVRERYHFLRVSLTRVAHLVFANSHESNDLKMVENVSSTP FT KKGVLEDPSTLTPEVVTPRTPGHRIIKAKGAYTKDRSAKRRRGRIEIERHLLGEFDDNV FT GSNSLLDVPLYSLEAEPLSPSVMLEDESMEGINQSPQGISVEKLGKEDNRSRSSTPASP FT SLESHEFSESREAGLSQSNGFEARSHGTGFDEYFDKFSQRKTSSNTLSQLPVLDNQVYL FT DTVKEICTETEQHTQTLVHFQSRNFHQWYFELVNNFNLLFYGFGSKEHFLSSFVEKKLP FT CFPIFVVKGYFPQLQLKNVLSSFLEFLEVTPAASVSDMLIQSLSIINSPSFSLGKIVFL FT VHNIDGESLIDERFQSALAAIASSKNVYFIASVDHVNFALLWDTSLESSFNFVMHDATT FT FARYYNETTYENSLGIGRANVSNKEKAIKHVLYSLPANSRGIFKFLLIHQLERMMDTQD FT FDARQGEKVGIEYRSFFQKCSAEFLCSNEPNFRSQLTEFFDHNIIESKRDVSNSEILWV FT PYPKNLLEILLEDMMEDV" FT CDS complement(12354..13460) FT /codon_start=1 FT /db_xref="GOA:O74369" FT /db_xref="SWISS-PROT:O74369" FT /label=SPBC685.10c FT /note="SPBC685.10c, len:368, SIMILARITY:Saccharomyces FT cerevisiae, YEK9_YEAST, hypothetical 53.9 kd protein in FT afg3-seb2 intergenic region., (477 aa), fasta scores: opt: FT 661, E():0, (36.0% identity in 364 aa); SIMILARITY: H. FT sapiens, O60906, NEUTRAL SPHINGOMYELINASE, (423 aa), fasta FT scores, opt:338, E():4.2e-16, (24.7% identity in 381 aa FT overlap)" FT /partial FT /gene="SPBC685.10c" FT /product="putative lipid metabolism protein, FT sphingomyelinase family similarity" FT /protein_id="CAB39367.1" FT /translation="QEIRNLVSCNLVYSRFFHSAAMGAGLAMFSKFPIIESSMNKYPLN FT GRPQAFWRGDWYVGKGVATASLQHPSGRIISLFNTHLHAPYGKGADTYLCHRLSQAWYI FT SKLLRAAVQRGHIVIAAGDFNIQPLSVPHEIITSYGLVNDAWLSVYPDQVEHPPNRFSM FT NDKELVEIAGTTCDSRLNTWRENISSKDMDDFVAKRLDYVFHSPSTCEAKNAKVVFLER FT VPKLDCSYSDHFAIETVLSIKLQPIPVQETRVSYSIIDDTLGITYQYMARERLHMRLRI FT AHLLISIPLIIGVHVAIAWCDPAWLKVIILFFTVMLTIAAVVNGFCIGLLFGRWEFNGL FT LEFVAELKEQKLLCKQYLVDHPLPFAKS" XX SQ Sequence 13460 BP; 4268 A; 2469 C; 2393 G; 4330 T; 0 other; attgaaaaag cttctatgca cgctccagct aaaggtagga actcgtctat gcaggagccc 60 tcttctaaat caccgttact gaaaacgcct aagtcgaatt attttcctgg atatggatcc 120 ctatcgccta acacgtctgt ggaattacca gaaataaata gcgattattc ggatgattca 180 gatgatgaag gtaataagaa aaaggtaaat ttaccatcgt gggctgaatc acctgagtta 240 cgagagcaat taaaacgaca acaaaagtgg gatccagaca aaattttcgg tatgattaaa 300 cctctgcaaa tggatgagta tttccgatcg aaggatcgat cgaagattcg atttcgacca 360 agaagctctt ctgctgactg gtcatctcaa gatcgtttga cccaagcaga aattgataac 420 tataagaaaa acatgggttt tttatagagt tctggatttt tgaaatgaat tttgtatatt 480 aaagaactgg gaaattaata gagaaatact ttaatttatc ttatgaaaca atttgcatag 540 agttagatga caaaattaaa ttagattaaa ggtttttgaa tctactaata acaattataa 600 tgaaatagac ctccatctta gacattgaag catcgctgat aattagtttt tgtgccgcaa 660 taacattagc cattaacaaa cgtagtttga cattgccaga aacttaatct acttgttcaa 720 agttttaatt tctttgaacg aaaagaaact gtgaaagtac ttctatcatc aatagtttag 780 cacagtttac attgctagtt ttggttacac tagacattta cgttccacca ttttaataaa 840 actagtgtaa agctttttta ccgtctggtt ggttacttta aaaaaaagaa ttattctatt 900 aaatgtgtga actaacgtaa cccgcacctg aataaatcat cctaacgaaa cattaagtct 960 atggaagaat atgaagattt tgagatggaa gatttgagcg aattagtctc ttatatggac 1020 tatcttgaaa cacaaagatt gactattagc caattggatt attctttgag cattccacca 1080 caattgctcg taagcaggga aatttctaaa ctagaggacg aaggtacgat atatttcact 1140 caagtcatga accagacaag ctaataatca acgttcagtt tgtgaaatgt taaaagaaag 1200 ttcattattc caactgtttc gaaaacacaa aggttattta aatgtaaggt ctttctgaag 1260 tttgtgaatg attttttatg gttttgtcaa cagatttata atagcggatt gagacaatgc 1320 tattcttagc ataatcattt acaagcatta atcaattgca tgtttttcat ttactgacag 1380 aacctattat taggttactg accttgtttt acctttgtgg tgtgaagtgc aacatgaata 1440 ctatttattg agaagaatta aaaaaaagac cccgaagatg gaacgcggaa ttaagctgca 1500 tcaaatactt gagtatgaaa cctcccctcc atcagaacgt cgagtgcttg atcggacatc 1560 caaggaggaa ccatgggcat tacgattatt aaggcaatta gaaggtatta tgttattaca 1620 aaaaaatgga attacaagag agtttccaat ctggggatat tacaaagagt cctcaatttt 1680 tggcattatt gatgaaataa gtctaaacaa cccttcaaaa agcaatttta atagtgacat 1740 ccgcaattac ttcaatttca aaatgtacga tttaagtttt gttgacaata aaactcggtt 1800 ttcttccaga aagcctggag cttcacaaat tcttagttcc aaagtacaac taatgtatta 1860 cgttcatctc tttttaaatt atttcccctc tctaggagaa aaacaacaaa gcatttttag 1920 ggatatttct cctacatatt ctaacagact tcattctcaa agtcactggt ggaacatgtt 1980 tttgagccaa ctatcgctgg acggcaccaa ggacttgggg ccgaaatttt tagagcaatc 2040 aattctttca attcccgata ttcctgaaga tgtctttgca ggtcataact cgctaaatgg 2100 actttatgca ctcgtatttg cttcagctaa gaagttgcat ttacgtctta ctgatgacaa 2160 tcttaccatt gtaagtgaca caaccaatta actcgaaaat tatttcgtta tcattacaac 2220 ttttaatttt ttttacgatg attagaatcc ttttcgtaat atgattgtct gaactatcgt 2280 tactaacatc ttttactagg catacaggaa tgacaaaaca ggagaagtgg tatacaaaga 2340 caaattctct tttagcaata aattattaga agcttcctat actaaggctt atcaattttg 2400 gcacaattta agggagcctg aaggcgttcc tgcagaggaa gtctacaaat gccgaagttg 2460 tgaatttcag aaagaatgtt ggtggctcaa aaagaaacag tattaccctc tttcaagtcc 2520 gtaattggga ataatgacag ttgaaaagat tcccagaaca tagttggtca ttggcatcct 2580 atatgtttag caacgatttt cttccatcat attttgatag ggctgctagg ttgttgaagt 2640 catcaacttt taattttttt atcaatcttt aagaggcaat tgaaatcatt tctatgtaat 2700 cgatataatt tgtgttaaat ctctatttaa aagagcatgg tttttttatt gcattttcta 2760 gatctcggtg acgatgcaat tctttggtag cttgtttgtt tctctcctag gtgccgcggg 2820 tctagctaat gctctaccat caccaggcat tcctagtaat gacaaagatt tggccattta 2880 tcctcctgtc gatgccaata atactacttc tcaagatgtc gttatagacg tagttctatt 2940 taacggtact gaatggattt cctctcgcga gagattttca cttgtttcac caggatgggc 3000 aagcatttac ggtccatcgg gtcagagttc tgttgggtca gtactttata gccctatttt 3060 ggattacatt gacaatgcgc aactgttagt tttggacgac gtgtctttcg gtgtcttctc 3120 ttccctggcc aattctgtta caggttatgc ttccaactat actcagtcat ccagcaattc 3180 cacttccaca atgaattcaa ccggaagtgt ttctggagga tcagtctatc caaccaattc 3240 aaccactaat agttccattt catggaatag ttcaacttct gctgctacaa acacaagctc 3300 aagcagtagc tcaagtagcc agagttcagt cgttagcgta aattcggaaa tattttctta 3360 tttcggttta agtcaacaat atgttaatta ctctacctct aggctttgtg ttgttggtac 3420 ccctagggct aatatgtcta cggtttctgt cacgaacaat ggatcggcag tctccaacta 3480 caccgtcaat acgaatggat ggacttcctc caatttcaag tgtgtagacg atgtggttgc 3540 aaatattttt ggattagatt tttacacagc tgctgtgctt tctgaagtct ccatccttag 3600 gagctttgca ttatgtaatg caacgactag ctcttcactt ttccgtcaaa ttgcaagtta 3660 tggtgtctat ggatctttcc atttctcctc ctctgaaagt ggttcttttg ctaaccttat 3720 tggcacaaat aactacttta tgactgatgt taaatcatcg tctgtagtca tcgttcaaag 3780 tgaaacatct tgtagtatta atagcgcctc tatgtcttcc aacacaactt atttctactg 3840 gaacagtaca tcttccctat catctagtgt gtttacaaat actacatcta gttcaaatag 3900 cacgaactct tccattccga cgacttaccc gtctaattca actacctatc agaacattac 3960 gactagttat ccatggagtc agccagttgt gaatattaca gattatctca gtgacaatgg 4020 agacggtcat ttcgtgctag ctggcgatgg aaatcaaact attggagatt tttatgtaat 4080 gaattggact acaattgcta gcggggaata cttggttccc ttcaattatt aatcacttaa 4140 tttactttca aattaccgac agtgtgtttg gtttaatgtg aaaagtttat attcctaatg 4200 aattattttg aagtttaaat tcatttggca catagcatgc ttttttttaa tttttcaatt 4260 attgctaaat cttaataaaa aatatttttc agaacgattt ttgattttaa ataattcaca 4320 atgatctatt acactattaa cataaacaat ttttcatttg ctaacccttt caaattcgga 4380 ggaaacaggg aaatgaatgc caaaatggta aagcatgttt taccaacggt tcaattacat 4440 gaagtattac cattctgaaa ggtgttgaat tctacaaatt gaagccggac tgtttcatct 4500 gtgacgggaa tatattaaat acaagaaaaa atataagaga cttgaatatg tgaaagagtg 4560 attattcaag tttctctaaa gcttcaattc tttcaagaac agattttttg tttgattctc 4620 caatttcgcc accaaggatt atctcgtcaa gaatagcact gactttatag aaattgaaaa 4680 taaggtctaa ttcacagaca ttcccaaaga aggaatcaag aatttctaca aaaaaatgaa 4740 tcatctctag aatggctaaa tcattatcgg ttgaatccac acaaaaacaa aaataaaggc 4800 cagcataccg acggtataca agcttgctgt tctcccattc taggaaattt gcctgaaatt 4860 tttggtttct ttgagatatc aattgatgga tacgagcctt taatcgaact ttttcatcat 4920 catcgaatgg cacgtagtat ttagaaagtc gattctttcc atgccggttt tgtatcaaaa 4980 tgaattgaat cattcccgat caatgaatgt aagtaaaact tcactcttaa tgagcaatta 5040 tagactatcg aaattaaaga attacgtttc aagccgcttt atgatttcgt attttgaaaa 5100 gtattgtatt cgtaaaagga aatttcactt tcatatgcaa ataaaaacgc tacggaaaac 5160 tgagctcgaa attgctctga atgagagttt ttcagtaaat atgcatttgg ccaggatgaa 5220 taagcacacg aagtttctct tcgtagtgcg cgcagttcat caaatgtatg aataaagcat 5280 gaaattacgt ctcggtattt aattttattg cgcactgtag tgcatcttta attttacaac 5340 cgaggtacac cacttcatcg taatactctt tgatattcaa gtacagactg tttacctgat 5400 ttgaagatcg tgaagggctc tacaaaggcg gaaacgacta tttttaacta aaaatagatc 5460 cttagacttt ttttaggcta attcttcttc cattcctaat tgccttcaat tatcgctttt 5520 atttgctcta ttatttattt tctaaggaag tttcgaaaca atcgaatagc ggatttaggg 5580 gactgttcac ttccttcgtt taaaaagaaa ataaattcta aacaaattca attcaactaa 5640 agcttataaa gcattttttt aaactgcagc atccttttaa acaaatttag tcggtaaaca 5700 aatttcactt agaaaaagct ttgagacctt tttttaagca gaaatcatat ttgtatttat 5760 ttcattcgtt tatgaaatat tatattcatt gacttgttga aatagtgaaa atacttcctt 5820 caacgttgtt caagtatctt atcaatgttg tgtctcatca acgcactaca gacgagtatt 5880 ggtaggactt tggatgtttc acacaattga gacctaagat gctcactatt aaacgatatc 5940 caggcgccat agaatttaca gttcatacga gcaaaagcta tggcacccaa atgtttgccc 6000 tctgctttat atcttttgtg atcggagcca caagtttggc tataggaaga tcgcctaaaa 6060 taattattac tttagtcgag ttatcttttc tactatccct ctttcacata atcagtggag 6120 taaatcgtat gtatcccctt atacgcatgt attttctaag aagcatttgc gagtgatcac 6180 atatcgttgg caatagtgaa ctatgcctac gttgcacagc aaataatgcg agcaattttt 6240 tcattggatt tattttattt tattttttta tttagaaatt gagctaactt acattttact 6300 agatgaatcg ctctttgtaa ttcgggattt aggtgttcaa acaaactgcc attcaatagt 6360 accatggaaa tcgtcttcaa aacttattcc tttggattca attcgagata ttttcattaa 6420 tgagggattt cgaaaatttg acgtttgtta ctacatggga attgctatcg agtctgaaac 6480 tgaaatccat gttgttttcc ctacacttct gccgagacat gatgtgttac aaaaagttta 6540 caaagaaacg gttattttgt tggctaacaa ttcataaaac ctataactag acacactctt 6600 tctatcataa ccacctttaa cgctaattac tagtttaacg ataattatct gcattttcga 6660 caaataatct tatgaagaaa ctaaaataaa attcattaat ttcgttttat attgaatact 6720 ctgtaatatt caatggtgga taaaaaccat tttgcctttg cgtagattat ggtaccaata 6780 aaatgatata ttcaattcaa tgatccatgt ttaacgaata aaagatacaa tgtgatatac 6840 tggtattgcg agcataactc agctcaccaa aatacattta acgaggatgg tctacatcta 6900 tccggtttgt agcgttgcaa gaaggcagtc acagtttcag gtctgggaat ttggttagaa 6960 aatgagcgac acagctcatt tgaatacttc atcttagcaa tggcacaggt cggacgcccc 7020 aacatactga atgctactgt aagtcaagaa atatagacag aatgattagt taaagtactg 7080 acactgaatt taggatgagg acatcaagca gctcctcgct gccaactgcc acattggctc 7140 caagaacttg gaggtccgca tggataacta cgtctggaaa cgtcgtagtg atggcgtcca 7200 cattttaaac cttggtaaga cctgggaaaa gttggttttg gctgctcgtg tcatcgcaac 7260 tattgaaaac cctgccgatg tttgcgttgt ttcaactcgt acttatggac atcgtgctgt 7320 tttgaaattt gctgctcaca ctggtgctac tgccatcgct ggccgcttta ctcctggtaa 7380 cttcaccaac tacattactc gtacttaccg tgagcctcgt ctcatcgtcg tcactgatcc 7440 ccgtgccgat gctcaagcta tcaaggaggc ttctttcgtc aacattccag ttattgctct 7500 ttgcgatact gactctatat tgaaccatgt tgatattgct atccctacta acaacaaggg 7560 ccgtaaatcc attggtttga tctggtacct cctggctcgt gaagttcttc gtgttcgtgg 7620 taccctttct cgttccgctc cttgggatgt catgcctgat ctttacttct accgtgatcc 7680 cgaagaagtt gaacgtgaag aggaagctaa gaaagctgct gctgccgctg ccgaggaagc 7740 tcaagtcgaa gaggctgttg ctgctgctga attcgagatc actgatagtg ctgctggctc 7800 tgttgatcct aatgttcttg ctgccgctac tgctggacaa gtcggtgaga atacttggga 7860 aggtgctggt gactggaata ctactggtgc cgcccaaact agtgactgga ctgctgccgc 7920 tgaggctcaa taaatgtttt ctctagtttt ttaaaataaa aataccattg tttgggtagt 7980 gaaaattgat tatagtagtt gtttattttt caaattaaat ttttagactg tagactttgt 8040 aatcattagt agagtaactc cgtctatatt cttgtacatt tttcggtatt atttgttttc 8100 atgcaaattc cgcgcatatt ctccatagca tgacaaaaca cagtggatcg attgtactga 8160 acacccgcta caaataaatt tacaaatgag agacaaaatt aatatcatac tttaaggaat 8220 caactactaa gctgaagatg ctgaatactt gaacttttat ttgagagtac gaaaattaga 8280 atctcaaagg agtgaagaac caggcagact ttccggattg gtacttctct tcgaaagtct 8340 ttttgacagt ctttctagca gctgagcgtt gagtgggctc cttgaaggtg tcagcagtga 8400 taaggccctt aaggttgtcg agttcaagag cataacgagt aggcatcaag tggttgtagt 8460 tgacaacctt gataaaaggc ttgactctag aacgcctagc aatgcgcttg gcacccatag 8520 atttggtgac ctttaaaggg tagcgctcga caccagcaac gacggcgtgt ccaaagggat 8580 gggactttga gccttggtcg atggcttgaa ggattacaac cttcttaccc gcaaaacggc 8640 ctcttgtgat aagagcaacc tattttgaaa tgttagtaaa gcgtactact tagtaaatca 8700 tacctttcct ggcttgagaa tcttcaccat tgttgtgttg taggacaatc ataggtggac 8760 gatttctcga cagcacgaaa ctttgtgact gcttttaata tgtttgcgca atgtaggcag 8820 cactgcgatg aacccgatta aaaagagttt cgtaaatcaa tattcagtaa caataacaac 8880 actttcttat tctatttacc gaatatagcg aagcttcaaa attcagccct ctctaataca 8940 ataaatattg gatgtttagt tctcactgat acattgatag aaataaagtc atttatgttt 9000 attttaaaga gaaaataaat ttctgagaat acttatactt gcatatggaa aggccgtatt 9060 aacagagttc ggtaccgata tgggattctt aaaacactag aaatactata ataaggaggt 9120 tttatctaca taattacata catactttgc ttgagcgaaa acagcttcta tattttaacc 9180 aataaatctt gaatattaaa caaaataact taaattattt catagatttt ttaacaatat 9240 ctggaactag cttctcaata tcctagtccg tttgctccgt agctttcgaa aactcaaagc 9300 gctcaacgaa gttgagaatg agcatcgtcc tttccacaac ttcaagttaa tttgtttttc 9360 tgttttcctt acttgaatag ttgatattta ccatggtacg tattttagtt ttaaaaaatt 9420 taattaacta ggttcctcaa ggtgcttgtg aagtttaagc gtgaaaagtt aactgttatg 9480 ctagaagttc aaccaggcct gtccgtttac gatgcagtaa agcttttaaa aagcgcgctt 9540 aacttacctc cagattcgat tctaagaatt ggatcttttg agcaaaatga ctgggtagca 9600 atggaaaatg atgcactttc aaaaaccatc atcactaata acactgaata tgcatttgca 9660 gaaggagaag aaccactact agtggaaaag cctaaagatt atgatgatat gtctgaagag 9720 atttcccctt aaaacacact tcattcatca gctatctatc aattggcata catgtttcca 9780 cactcaaagt gttataaaaa ctatttatta acctgagact accagatagt gctagtatta 9840 tttcgataat atttcttctg ctctttgtta caatcaccaa aagacatgtt tcccaagcta 9900 tggaatactt ttaatcccga atcgtcattc gaacaaaaat aatacatttt ttctgatcat 9960 attttcgaga attttaatcc atttcattta tttgctagta tggattcatt ttacgaagta 10020 gaacgtatcc taaaatgcta ataaacgagc acgtacgaga acgctatcat tttcttcgtg 10080 tctctcttac aagagtcgca catcttgtct ttgctaacag tcatgaaagc aatgacttaa 10140 aaatggttga aaacgtatct tcaacgccta aaaaaggagt tcttgaagat ccttctacat 10200 taacaccaga agttgttact cctcgaacac caggacatcg aattataaaa gctaagggtg 10260 catataccaa agaccgttca gctaaaaggc ggcgaggtag aattgagatt gaaaggcatt 10320 tattgggaga atttgatgat aatgttggtt cgaattcact acttgatgtt ccgctctatt 10380 ctttagaggc cgaacctctt tcaccatctg tgatgcttga ggatgaaagc atggaaggaa 10440 ttaatcaatc tccccaaggt atttccgtgg agaagctagg aaaagaagat aacagaagtc 10500 gttcttccac acctgcctcg ccatctcttg aaagccatga gttttctgaa agtagagaag 10560 ccggcttatc tcaatcaaac ggctttgaag cgaggagtca tggcacgggg tttgatgaat 10620 actttgataa attttctcag cgaaaaactt cgagcaacac tttatctcag ttgcctgttt 10680 tggataatca agtataccta gatactgtaa aggaaatctg tactgaaacc gagcagcaca 10740 ctcaaactct agttcatttt cagtctcgta attttcatca atggtatttt gaacttgtga 10800 ataattttaa tttactgttt tacgggtttg gttctaaaga gcattttcta tcttcttttg 10860 ttgaaaaaaa acttccttgt ttccccattt ttgtcgtaaa aggctacttc cctcagttac 10920 agttaaaaaa tgttctttca tcctttcttg aatttttaga ggtcacacct gctgcgtctg 10980 tatctgatat gttaattcaa tctttgagta ttatcaattc tccctctttt tcgttaggaa 11040 aaatcgtgtt tctggttcat aatatcgatg gtgaaagtct aattgacgaa cgatttcaat 11100 ctgctttggc ggccatagct tcttcaaaaa atgtgtattt cattgcttct gtggatcatg 11160 taaattttgc acttttatgg gatacatctc tagagtcttc gtttaatttt gtcatgcacg 11220 atgcgactac ctttgcacgg tattataatg agactacata tgaaaattct ttaggtatcg 11280 gaagagcgaa tgtgtctaat aaagagaaag ctataaagca cgttctctat tctttgcccg 11340 ccaattctcg cggtatattt aagttccttc taattcacca actcgaacgc atgatggata 11400 ctcaagattt tgatgcccgc caaggtgaaa aagtcggtat tgaatatcgt tctttctttc 11460 aaaaatgtag cgctgaattc ctttgcagta atgaacccaa ttttcgaagt caactcacag 11520 aattttttga ccataacatt atcgaatcaa aaagagatgt atcaaattct gagattttat 11580 gggtaccata tcccaaaaat ctccttgaaa ttttgttgga ggatatgatg gaagacgtat 11640 aaaataggac ccttttagac taggttacgg aacattagag tataatttat gtatttcttt 11700 gtaaatagat atttcacgat ccataataat aattgtttgt taaaaattta ctattttttt 11760 ttatcaatta tgcacattgg tgcaattgta tgtagtattt cgaaagcaat tcattatcga 11820 tatttaacat agtaattgaa tacagtatag ttttaggatt tcacatctta tatctaacca 11880 tctctaataa tcctagtatc agcattgtaa atacttaaat tcaaagaaca agaaccatat 11940 atcgttcttc atgaagctag tacaatctca atcatttgta attacagtaa tctgttcacc 12000 aaacgaacag aatctttaaa taatattgat ttcatccctc taaatccgaa gtcgcttttt 12060 gtaaatacgc gtttacaaag acaacgagtt taaaaacaat gaccatttat aagtatacct 12120 aaatgaaaaa caactggttt gagtcaaata ttaccaaaac caaactttaa aaaaataaca 12180 cagaaattat caaaggttgt ttgtaaaaat aattaaaaaa aaacaattag gagtcgctag 12240 cctcagcatg caaagctgtg ataaaaagct tagataaaaa tgatgggaaa ttttaaatta 12300 acaatgtggg ataaagcggg ataaagaagg catattaaca gcaatcggta aatttatgat 12360 ttagcaaagg gtaacggatg gtccacaaga tactgtttgc aaagtaattt ctgttcctta 12420 agttcagcaa caaattcaag caaaccatta aactcccatc taccaaatag caatccaata 12480 cagaaaccat tgactactgc tgcgatggta agcattaccg tgaagaaaag gatgataacc 12540 tttaaccaag cgggatcaca ccatgcgatg gcaacatgta ctcctataat taagggaata 12600 gaaataagta gatgagcaat tcgaagtctc atatgaagac gttcacgagc catgtattga 12660 tatgtaattc ctaaagtatc atcaatgatg gaataagata cacgagtttc ttgaactggt 12720 attggttgca attttataga caaaacagtc tcgattgcaa agtgatcaga ataactgcaa 12780 tccaatttgg gaacacgttc aagaaatacc actttagcat ttttagcctc acaagtggaa 12840 ggagagtgaa aaacgtaatc gagacgttta gccacaaagt catccatatc cttactagaa 12900 atgttctcac gccaagtgtt gagacgggaa tcacaagtag taccagcaat ttcaaccaac 12960 tccttatcat tcatgctgaa acggttaggt ggatgctcaa cttggtcagg atagacagac 13020 agccaagcat cattgaccaa accataagag gtaatgattt cgtgaggaac actaaggggt 13080 tgaatgttga agtcaccagc agcgattacg atatgaccac gttgaacagc agcacggagc 13140 aatttcgaga tataccaggc ttgtgacaga cggtggcaca agtaggtatc agcaccctta 13200 ccgtatggag catgcaaatg agtattgaac aaagagatga tccttccaga gggatgttgt 13260 aaactagctg tagcgacacc ctttccaaca taccaatcac ctctccaaaa agcttgagga 13320 cgaccattaa gtggatactt gttcatagaa gattcaatga taggaaactt cgaaaacatg 13380 gcaagtccag cacccatagc agcactatgg aaaaacctag aatagacaag attgcagctc 13440 accaagttac gaatttcctg 13460 //