ID SPBC661 standard; DNA; FUN; 2500 BP. XX AC AL031741; XX SV AL031741.1 XX DT 24-SEP-1998 (Rel. 57, Created) DT 16-NOV-1999 (Rel. 61, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c661. XX KW membrane transporter. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-2500 RA Wood V., Rajandream M.A., Barrell B.G., Volckaert G.; RT ; RL Submitted (24-SEP-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Katholieke Universiteit Leuven, Laboratory of RL Gene Technology, Kardinaal Mercierlaan, 92 blok F, B-3001 Leuven, Belgium XX DR SPTREMBL; O74829; O74829. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c661 is overlapped at the 5' end by cosmid c530 and at the 3' CC end by cosmid c36. XX FH Key Location/Qualifiers FH FT source 1..2500 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c661" FT /map="IIL" FT misc_feature 1..849 FT /note="nominal overlap with cosmid SPBC530, EM:AL023634 FT S.pombe chromosome 2" FT CDS complement(1..1349) FT /db_xref="SPTREMBL:O74829" FT /label=SPBC661.01 FT /note="SPBC661.01" FT /partial FT /gene="SPBC661.01" FT /product="putative membrane transporter" FT /gene="SPBC530.15c" FT /protein_id="CAA21092.1" FT /translation="MAERTSESSSESASFDLEKQQSNHHDRYQSSVSSELEESLKKYPV FT ISNPQDFIVTLDGPDDPDLAVNWPLAKKLRNVAVMGSACLCAGFGSSIFSGAVPEVMVK FT FHVCRTVALLGISLYVLGFASGPVVWAPMCELFGRRRPMIIAVFIFCIFHIAVATAKDI FT QTVMICRFFCGFFGSSPITTVAGSFSDMFSARTRGLVIAVYSAIIFNGPLMSPIVGGFI FT GKSYLGWRWTSYITAIMGFTAFTSMIIFHRETYTRTITEIRASKVRVLTGNYCLHAKSE FT EEPLEFSYFFHKYFTFPLRLLIFEPILLVVSTYTAFVYGILYGLLEAYPVIFGESRKWR FT LGVESLPYLAIFVGVCIGCSSVALFQPYYFKKMDENKGRPVPEARLPSMMIGCIVFPIG FT IFWLAWTGNYPWIHWIVPTLAGSFIGFGIITIFQQTINYIIDCYSGCSASA" FT misc_feature 1774..2500 FT /note="nominal overlap with cosmid SPBC36, EM:AL023589 FT S.pombe chromosome 2" XX SQ Sequence 2500 BP; 922 A; 461 C; 474 G; 643 T; 0 other; gcagaagcag aacatccact ataacaatca atgatataat tgattgtttg ttgaaaaatt 60 gtaatgattc caaagccaat aaaactacct gccaaagttg gaacaatcca atgaatccat 120 ggataattac cagtccaagc aagccaaaag atgccaatgg gaaagacaat gcaaccgatc 180 atcatggagg gaagtcgtgc ttcaggaaca ggtcttcctt tattttcatc catcttttta 240 aaatagtagg gctggaacag ggcaacgcta gagcatccaa tacaaacacc aacaaaaata 300 gccaaatacg gaagagattc aacacccaac ctccattttc gactctcccc aaaaataact 360 gggtatgctt caagtaatcc ataaaggata ccatatacga aagcggtata agttgaaacc 420 acaagtaata tgggctcaaa tattaaaaga cggagaggaa atgtgaagta tttgtggaaa 480 aagtatgaga attccaaggg ctcttcttct gatttggcat gtaagcaata gttcccagta 540 agtactctca ccttggacgc tcgtatctct gtaattgtgc gggtatatgt ctcacggtga 600 aatataatca tagatgtaaa tgctgtgaat cccatgattg cagtaatgta ggacgtccat 660 ctccatccca agtatgattt tccaataaaa ccaccaacaa ttggactcat taacggtcca 720 ttaaaaataa tagcagagta aactgcaatc acaagaccac gagttcgagc actgaacata 780 tccgaaaagg aaccagcgac agtcgtaatt ggtgaactac caaaaaatcc acaaaagaaa 840 cgacagatca taacggtttg gatatctttg gccgtcgcaa cggcaatatg gaaaatacag 900 aagataaata cagcaataat cattggtcgt ctacgaccaa ataattcaca cataggagcc 960 catacaacag gtcctgaggc aaatccaagg acgtaaagac taattcccag taaagccacc 1020 gtgcgacaaa cgtgaaattt gaccataacc tcgggtacag cacctgagaa aatagaagaa 1080 ccaaaaccgg cgcacaaaca cgcactgccc ataacagcta cgttacgaag ctttttggct 1140 aaaggccaat taacagccaa gtcagggtca tcagggccgt ctaatgtgac tatgaaatcc 1200 tgaggattgc taatgaccgg atattttttg agagattctt ctaattccga agagacagag 1260 gattgatacc gatcatgatg attggattgc tgtttttcaa ggtcgaagct agcactttcg 1320 cttgacgatt ctgaagtacg ttcggccatg agtatagaga gatgggttta aacaccttcg 1380 ataatgttga attgagcgac aaaggaagat tagacaagcg tgtgcatagg atataaaata 1440 atactataca acgcttttat gatagtaaaa actggatata gtgaaatcaa cccactatcc 1500 gtattgaaag gaaatatgta taactttcaa aatgcaaaaa ttgaaataga gaaaagttga 1560 aagaaaaaaa agacagcaca gctgtatttc aatctaatat cgtatcacaa aagagtaaga 1620 tacaaaagaa ggatgagaag gaggtatatg tatatttttt aggttaattt ctgttagata 1680 tacacatgta tatatattat atatatatga attttatatc acaaactcca atctttattt 1740 cttagataaa aaaaaataaa aattagcagc gaagatcaaa tatttggaag actggtgaat 1800 caaccaatct atggaacata ccaccctgat tatcaaaagt aaatcagacg caagcggtga 1860 aaaaagaaga accgaagata tccaagaacc aattctagtc tgacggtgta attacagaga 1920 atttgttaat acgttcagct atttctattg cactgacaac agtattaaac tttaacttct 1980 tagaataagt atttcttgaa gaaagaagct aatagctctt tttaaagatt aaaaccgact 2040 ggagacattc aatgcaacaa tttgctgcga aaaggttctg gggagatagc gatatatcga 2100 tagaaaattt tgtacccgga ctcttatatt ccgattttgc cagcaaactc tgagcctggc 2160 tgcaacacac agtaacgaga tgcgcaaaat atataaagcc taaaatcagt ttctataagt 2220 ttataaacta tctgatgcta ataagatatt caactctgag caatcaatgc caaactacct 2280 caaatagcgc aaatgcgtta cggtgttagg ttttgaaatt cgcttgtcaa tccgttttaa 2340 taaacgtgga tcctttgaac aatccaatta tattaggcaa atggtatagc cttgattttt 2400 agtgtcggta aaaggatgta cctaatcagc gaaaaactca agacaggaaa aaaagttccg 2460 aggctttgtt ttagcgtaga tagtcgagca agccgtcagc 2500 //