ID SPBC633 standard; DNA; FUN; 9838 BP. XX AC AL391715; XX SV AL391715.1 XX DT 29-AUG-2000 (Rel. 64, Created) DT 29-AUG-2000 (Rel. 64, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c633. XX KW centormeric; Chromosome II centromere; tRNA Ala; tRNA Ile; tRNA Val. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-9838 RA Wood V., Gwilliam R., Rajandream M.A., Barrell B.G., Skelton J., RA Churcher C.; RT ; RL Submitted (29-AUG-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c633 is overlapped at the 3' end by cosmid c21B10, CC EMBL entry SPBC21B10, accession number AL121794]. XX FH Key Location/Qualifiers FH FT source 1..9838 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c633" FT /map="IICEN" FT misc_feature complement(1..7418) FT /note="cen2 central region" FT tRNA 4645..4727 FT /note="tRNA Val anticodon AAC, Cove score 64.30" FT intron 4683..4691 FT /note="intron tRNA Val anticodon AAC" FT misc_feature complement(4744..5404) FT /note="single stranded" FT tRNA 5388..5461 FT /note="tRNA Ala anticodon AGC, Cove score 67.24" FT tRNA complement(5535..5608) FT /note="tRNA Ile anticodon AAT, Cove score 66.44" FT misc_feature complement(7419..9838) FT /note="cen2 repetative region" FT misc_feature 9738..9838 FT /note="nominal overlap with SPBC21B10 S. pombe chromosome FT 2" XX SQ Sequence 9838 BP; 3508 A; 1428 C; 1481 G; 3421 T; 0 other; gatcagttgg taaaacaaat ctattataga aattatatta atttcagaga gcctcatatt 60 ttacgcaaat tatgaaggta aatattgtaa tcagaccatt tgcgaaacag taaatttctt 120 ttttctgttt ggttttgctt gtgatttggt tcatggtttt tattaatatt ttcaagatag 180 tttctaaaga tcatccagaa caatttctat tccttcatta tttcgttgaa atgaccagat 240 caaaataaaa ttctcgaaat aatatttttg caatttttta tgttttattt tatatgcaaa 300 taaaatgttt acatggaaat cccatatatt taaaactgac taaagcgtat ttaagacgta 360 atctaggacc cctacgtttt ttgtttcttg cagaaagttc gaaaaatcgt gcacatttgt 420 gaaaaggttt agctctacct atcgcaaata aatgctgcat caaacgcaat tgctgctatg 480 tgggtctgta ttgcctctcc cttgccagta atgtgtattt catcttgttt atttgttttc 540 ttatcgaaat taacttaagc tttgtccaaa tgctaattat taaccacttt gggtgtatac 600 gaaacacata ttttcaaaac accgcaacga aaaacaaaaa attgtccctt aatatttgtt 660 gattaaagta tcgtgaaagg ctttttcgga aataatctaa aagaatcttt aacacctgtt 720 aatactttac aaactaatcg cgagaaaatc attaagagtt tagaataaag tttcctagca 780 atattttgct atataaatga ttttctggtt aatggttata gcagaaaaca cagacatttt 840 tgtacgtatg tacattaact atgtgaataa acatcttagt acgggttcat cagttcaata 900 aatattcaac tgtattaaca acatgtgcgt ccgtgaacat ataatgaaag tcaataatta 960 tcatgaagct atattttcag ttaaaagttt acccagtaga tagctctgaa gaaatttatt 1020 tgatgaacag tctaaaattc aatgcgaaat ccttttaatt aatagtgact aatttagtcc 1080 aagctttcgc tttagtgttt gcttactttt attttcccta ctaaaataaa atctaaaaat 1140 ttttattcct ggaaatcagt aaaactcatg atttcaaaga aaacagccaa aggtctcagc 1200 tgctgctagt ttgttcgtac gaaaaactgt tttcaatatt ttgactataa ctagaccact 1260 cagattgaat tttttgaaaa ttttgtacat tcaaattgcg tcaactgctt taattgtctg 1320 aaatacttga tttgctttct tgagtattca tctagtttta tcattaaagc taggtatttc 1380 caagggctcc taaaactaat gattagcgat gtaatatctt aaatatacca tttaagttga 1440 tgaatgtata gtatgtttta ctaggataca cggtagaaca catatacttg gatgttatgc 1500 atacacatcg tagtaattgg tccattgttt ggattgaatg tttagtatga gaactgtatg 1560 gtggtatacc aaaaaatttt tacgtttttg aaagtaaaga tatgattaat atctgcaatg 1620 ttttctttta gattatggaa tctgttacaa cagcttaaat ggtagtttaa ttaacaaggt 1680 atttactttt tattgttttt ttgcagctcc tccaaaaaat taactagcct agatcacaat 1740 tatcagtcaa tctgaattaa tagacgtaag aataacttct taaaaataca gaaaacaaaa 1800 aaatgacttc ataacgtttg tataaaccag atttaattaa cggccaataa gtttagttac 1860 ctgttagcaa aaaataattt taatactgcc agtgactcgt cacctttagt aaccctgtaa 1920 ccgtaattca gcctgtccat cgcaaaaggt aacccccatc agtctgtaaa tagtaatctt 1980 agtagtgctt caaatattaa aaacggattc gcattctctc aagatgtcga atatgcagaa 2040 aaaactttca ttattaatta ttaaacgtaa atctatttac aaatctcaac atttaaaagt 2100 tttactaaat ataatttaac cataaaataa ggttgatgtt gaattattct catgtacaac 2160 ccaaaaaaaa ggcaatccta catcattgga attttttaaa taaattcttt ggattcgaag 2220 gtctttactc gtttttaaaa aaattgatct gaattggctt attagatgac aatatcaaat 2280 atgtaccgac tcagttgacg ttacctttat caaatttatc gtactttaaa acttactaaa 2340 gcagaagaaa atatgctgaa ctatttgtgg tggacattga taatgtaaat tacacactta 2400 acgaaattat ctagttacaa atatataagc tactagtcaa taaaaagctg aaggctcaaa 2460 tcattacgta aaatattttt gatcgggaat acaaatacta acttcttgag aaagctgcat 2520 aaaaactagt gtcaatataa aaagcactag tttttcctaa tattgactgt tgtttacaaa 2580 tttgtttact gagatagcat tcagcaaatt gcaaaagcct ggttaaaaag gaagacgact 2640 attctaaatg gtatttgtaa aatgcttgct ttacttaatt aactaataac atagaaaaaa 2700 ctacttttaa ttttgatcat ataactacag atagaaaagg cagtcaggca accagttaac 2760 aaccttttag aagtaatgca tacttaagtt atgaaaaaaa aaattcaagt ccacttccaa 2820 tcccataaaa atgaaataaa gcaaacagca gtaaccttgt aaagcactaa actcattact 2880 agaaacagtg aggtgcaggg aataaatgta cataatacaa attaagccgc atataaggac 2940 gcagcgagca aaatagcctt cctaatcaaa ataactaaaa ataaagataa aagaagaaaa 3000 ttcgaatagt gtaaaattac acacagaaat acttttatgc gagactttct taggatatga 3060 attatattaa ctaaatagca actgactaat atgtattttg ttttgttaag ttaaaaactt 3120 tggcatttgt ttgaagtgat acaagtagaa tgatttcgaa ctgatgattt ttgataaaat 3180 ttaatatgaa cgctaatgtt tccatgagaa gaagtatatt ggtcaattag aaccaaatca 3240 aaattaaaag aatgttttgt atttgtaaat ctaccgaaaa tgttcaaatg catcatatca 3300 aagctcttaa atcagaatac aataaaaaag tgcctggatt aacaaaagtt atgatagccg 3360 tttataccct ttataatcaa atcaaacccc tgtttaccat cttactcata aattattgaa 3420 ggcgctaatg cttaaattaa tcacataaat ttcaagttac aatttccttt tttttttttt 3480 ttttttttat ttatatagta aaaggcaaag tacgaaaaca ggaatgtgat aaaactaatt 3540 ttgtttcgaa tcattccatt tttacattaa cccaagtatt catcgtaact aaataagtga 3600 aattttaacg taaactagtt aaaaataaaa acaagcaaaa aataaatacc tcgttagctt 3660 tacaaaattg atgagatgag taaattaaat ggaaatttta aaacacattt ttgtcgtttt 3720 atgcaccttt tttgaccgaa aattacatta ctaatactta tgatagtaag ataagaacca 3780 gtaaacaaag aaagtttaag ttcattactt aaaaaataac aaaacgttgt tcactgaaac 3840 ctcatgtggt tttaaagtac ctatctaaaa gcagtttcat aaacgatctg gtactaccta 3900 attgtattat tgtttcgccc taaaagtaaa cggtaagcac tttgcaacga ttaccggttt 3960 ataaaaatta ttccatggca tgattaattg gatcactatt tcttataact tttcaacatt 4020 taaaattgat taaacttcct acatttgcgg aactggttat aaaaaacgct ttatttatta 4080 tacctatact tatatcacgt cgccgaagtt gagtgttttt aaattggaaa gacaggaaaa 4140 gcttttcaat acttgaatgt acattgaatt gtagcctatg tattatatgc aagccatatt 4200 gctttgcaaa ataaagaaca agctcattat atcataataa aattttataa gtatttacaa 4260 tacttagttt aaacagtaga cataggaata aaaaatgtaa atattattca catgcataca 4320 tcgtagtagt ggtcgttcgt agctgataaa attgcttaaa taagtattat tttccattag 4380 aaaggacaga gttctgaaca tattatgctt acaagcttgt tacgatatac gtaagttaag 4440 aatgaagatt gttattgaag aatttctgat agtaaagcca tattgataaa tttcaaaaaa 4500 gttctatgca tgagataatg tagctaagat agttgaaaca tgcagataaa gataatttgt 4560 gtattcgtaa cttcagacaa tttgacgtta aggttcattt ataaacctat attacaaata 4620 aagctaataa taaaaccaac atctggtcgt gtggtttaga tggttataat ttctgcttaa 4680 catttacagt tcgcagacgg tcccaagttc gagtcttggc acgatcaatc gtttttaagt 4740 ttttaatttt ttacttttaa aaaagaaatt atatctgctt cattaattaa cgattctttt 4800 ggaatgaaat catttttact tttttaaata aaatgagatc agcaattttg gttggattaa 4860 attttttttt ttatttactt gttccattgt agttctcatt tttttcaaaa gtgtaattac 4920 taattatctg ttgtttgtaa ttaattattt tcgcatcgga tttaaataaa aaaaaaaaaa 4980 gaagagggaa actgtaaaaa taattatctt aagaaatatt aagtgctgaa aaatatacaa 5040 acaaaaactg aataaaaaat ttagttagtt aatgttacta atgaagctag tttgaagtat 5100 ttttgagtat ttggactaaa aagaaagata gttaatagtt aatttaaatc actagataaa 5160 acaaataaag aagcggagga taactcatca gtagtaaagg tgtaacaatt atttgcagtg 5220 gtagtaaata tatatatata tacatatatg tatgtatgtt gtatttgtat gtatgttata 5280 tttgtatact atgataagcg tgggtagtat caaagtgcgt tggaaaagaa taatagcaaa 5340 tagcaaagaa agtaataatc gactaacata gagtaaaaat caaccatggg catgtggtgt 5400 agatggttat cacgcttcct tagcatggaa gaggtcccag attcgagttc tggcttgtcc 5460 actttttgtt tatttttttt ttgaaattta ctgatgtata gtgtactgtg agattcatta 5520 aaaaataaaa aacatggtca cagccgggtt cgaaccggcg acattcgact tattagatcg 5580 acatgataac cgactacatc atgcgaccat tattgatggc acgagtaaaa taaattataa 5640 taacttctga tgtcacaaag atgtattaat aaaatattta ttttttcgca ataatcatta 5700 tttaattcct acaattttgc cattactttt atgcatattc tactacctta actaatattt 5760 ctttaaaagt aacattttta gtacttctag gttattacta ttccctatta ctttccaatt 5820 attaagtgaa tgctctcggt aatttattgc aatttttgga tcagcttagt aacgaaaacg 5880 tattaagtat ttatggaatt ccttacgtaa catacgtttg caggtttact gcattttctt 5940 agtaaattca caaccgtgac ttcattgatg ccattattat atgacttaca aactatacta 6000 ctcttcgcta tggataaact tttctttcgt accacctatc aacaatggtg tctataactt 6060 tacgtaaagc agtaaagcaa gtttaaatat ttcatcttcc ccaatcgcaa tccaataacc 6120 aaatgcatat ttggtttaca atggttttaa agattagaca caagcattgt tctcgtttgc 6180 cgatttctta atatgtcatt tttctttcag atgtcaaagt atatttttat tagtactatc 6240 atgtgagaca cggtcttctt aatttcgtaa ttgttactca gtttatttga taaagacatg 6300 attatctgat ttgtgagcac aaaggcatat tacgctctca cttggcacta attgtttaga 6360 ttaatatcca gttttgcttt cgtttaaaaa atatttaact tgctcactca tttattttta 6420 cgcacaaaca ttatttacaa cggaaattat cgaccatgtg atgtataaat gaacacaatt 6480 cagtcgttga attaataaac ttggaaaatt acgtaataga aataaaaaag caggtttata 6540 ttatatcttt gtagtcgatg atcatccttt gattattgta acacatatac acggatatgt 6600 aagtgtgttt aattaagcat ttatttgcta gtttgttaat gcatagagag ttttattctg 6660 ggtttggtca taataaatta acgatctcct tcagagaatc caaaattgta ggctgtattc 6720 aagtctctgg actaaaaaaa ccacaatatc attattattc caatttgcga cgactcattt 6780 tgtgatagga gttaatggat cctgtgacta tatacactac taaagagttt gaacaggcat 6840 tcatcaaaga tttgtcttca tgatttacta aaatactgag tgttaagtgt gagagagaaa 6900 aagaaggaat tgaacctccg cattcatttg tgcattctgt ttgccattat gtataaagta 6960 tcaaaagaaa gatgacaagg tagaggtcgt acaagttgaa aaaatcagat cataggctct 7020 tcaagtcaat tttgtatttt gtattttgaa agaatgggca agtaattttt aaaaggtttg 7080 gtgatttatt acagtttttt ttaatctata aaatcagtgt agccatcaaa agtgttgcta 7140 attaaactga tgtatatagc agcgatggta acgctctcga cgacagcaca tcgaccagga 7200 ttacaaaaaa cttgtgaatg cctgcaagaa agtttaaatt aacaaataaa acaaataaat 7260 gattgtgaaa aaccccgaca tgcattagtt ccaacttatt cattctgaat gcacgagaca 7320 attgtatatc caattataaa aaatttgagt aaataaaaaa aaatcgtcac ggtttacaaa 7380 ttcggtaatt tgttttccgc ggaagaaaag cattccggat gagaatagga tccaattgaa 7440 tagaagattg aagaaaatgg aaccggcgat tgagaaagac tacaagatga aaagccagag 7500 acaatgatta tagcccaaag gaagctaaga tacactaagt tatacgaaaa gcacgatagc 7560 ttattcataa acaatccaat ttcgaaaatt gtgttgtgcc agttacaaat tacagcaatc 7620 ggtgataaaa atcaaagctg actttgttat tggccgtatc ttttcgaaac tttgattcta 7680 ctgcttatgg aaccgttctt taatttgatt gtgtttggga gaagaaactg tatgatagta 7740 gtgtgtgcag acacgcaaga tgaatgaaat acttcgaaag gaatgtttat gtaaatactt 7800 gtggagactc agtttaactc cagcattttt tcaactatcc caataatccc attatttcta 7860 tttctgcctc acgtttctat tagcatagta tggttggaat tttgagttac ttctttgtag 7920 cattctttaa ggattcattt ttacaaacca ccaaatcatt gtgtacaagt tatacttagg 7980 taatcatatg catgctccgt tgcttatctc gttaccaaaa atctgtaaac taacactttt 8040 acaatagtca attaagatat tcgggttttt tagatgcagc ttctgtatag ttttcttact 8100 caaagcgcat tatttttcta cggatagatt ttaaggagca aaaacagtat catcagcttt 8160 tatcagtcat gtcgaatgtg aggattcttc atgctacgtt aaagaaacga gtcgaaaagg 8220 aagtgcggtt gacaacgact atttaaatgc taaggaaata aaaatgtagt aagtttttaa 8280 agttaccgat ggaaacgtag tattacgtat atggaatctt gcatcattat ggaaccgtca 8340 agttacggaa tgtaaatttc ctacgcttga tttgaggaag gaaaatcgta aaatctagac 8400 attgaatatg ttttttcaaa atggatagcc ttcatggtat ttgtataata ttatttaatt 8460 acagcctaat ttgatttaaa aatcacttct gcgactcata ctttttcgat ggttaaagct 8520 tgatttagga atatgggttt accgcagaac tctagctctt ccaatcaacc gttattacct 8580 cgaaaggtat aacaactcaa caagacctta tattgtctcc aattctatat gttacgttat 8640 gctgttataa acagaagaaa cggtgtatct gctgttgaat aagtttttgc attcttatca 8700 cttggatgta ttattacatt tcgttggttt gtatatgtac atatacttaa acatgtgtgt 8760 ttataaatca agtaccatat ttaaaaagtg tttatttttt ttatttttat ttactcaatt 8820 gttatgccac atactttaac taaagatata gtaagtatga gcaactggcg tacgtagaca 8880 gagaaataga gtagcaaaaa attagtcata aactgatcaa gtttattatt accgttataa 8940 caaagattat attcttccat tcatgtcgta gatgtgacgt caaccattcc cattgcctct 9000 tatgattagt attatacagc gaagttatat atatatatac aatagacaat caatatacat 9060 ataatagaaa agatgccaaa aaaactcggt ttcctgattt gttcctggtc atcagttaat 9120 gaattttact tgattaacta cgttgagcaa agaatgaaat tgatttaatt ctacagtaca 9180 ttgtgttagg gtaaatatta gaatctagaa acaaacatta gtttgtttgt ttgttttggg 9240 gagacgaatt attataaaca tcatttatca ttcttttaac tataataatt gtttaataaa 9300 aaaaaatata tgtttgtact tattttgtta ttgtacattt ccattgcaac atttaataat 9360 gtcatgttat catgctgtca tactacactg caactaacgt actgaccgat ttgatcgtca 9420 attttctacg gaatcgatga atcgaatgca tatgttcaaa tcgctcgcct tgccaatggc 9480 ctaaaattgc gaacctgaaa ctgaaatatc aaaagaagac tcaactaata cttaaaagga 9540 aatagaagga agatttcact catacctacc gaacgtatga ttagcataac atttcaaata 9600 tatatatccc ttattataat aactccctat taggattagc ccgatataaa aacatattct 9660 ctttggttat aaaaatccaa gaaaggacat ttttgttact aaatatacta aggcaaaaag 9720 cctcaatcaa aaattgcgat cccaattatt cagaatgata aatttgattg gatttatttt 9780 tggctatcat ggtaaggata tactagaggt ttaatctatt tgaacatttc tcagatta 9838 //