ID SPBC604 standard; DNA; FUN; 1922 BP. XX AC AL132720; XX SV AL132720.1 XX DT 29-OCT-1999 (Rel. 61, Created) DT 29-OCT-1999 (Rel. 61, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c604. XX KW . XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-1922 RA Harris D., Lyne M.H, Rajandream M.A., Barrell B.G.; RT ; RL Submitted (29-OCT-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c604 is ovelapped at the 5' end by cosmid c26H8, CC EMBL entry SPBC26H8, accession number AL031743, and at the 3' CC end by cosmid c32C12, EMBL entry SPBC32C12, accession number AL022172. XX FH Key Location/Qualifiers FH FT source 1..1922 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c604" FT /map="IIR" FT misc_feature 1..125 FT /note="nominal overlap with cosmid SPBC26H8 S. pombe FT chromosome 2" FT misc_RNA 732..962 FT /note="identical to mRNA from spc00524 and AU009915 231 1 FT but not associated with an ORF" FT misc_feature 1821..1922 FT /note="nominal overlap with cosmid SPBC32C12 S. pombe FT chromosome 2" XX SQ Sequence 1922 BP; 612 A; 341 C; 389 G; 580 T; 0 other; ctgttaaagc catgttagaa ggtgcagtag aatatgggga aatagagcga aattcaaatg 60 ggatatagta gtgtctatag tgaaatggta tattttgaac tggaaacccc tccccccact 120 tgatcctatt tggacccact tggagccaaa aaatagtgta tcaacggttt gcactcagga 180 ggaagatcac cgtattcttg ctaccccaca aagggatgaa caccccacat ggaaaactca 240 atactcagat ggtagaactg agaatatatt tcaagagcta gtacgagaat actgcccccc 300 gggagtatga tgcccccacc aaaggttttg gcatcccaca tcaaggatat taaaagattg 360 actagtggga taagaaaggc gagagtaacc ccacgttgct ctatgtggtg gtctctcgtg 420 acaagtcgtc tggagagacg gatgcgcaat taaaaagggt tgacaagggg aaattggaga 480 gttcacgagc tatccatggg attgtggtat attgtgaatt tatctaggtg tctgagtttt 540 ccgtggacaa tgcatctgtg gattgcatat tcaatatcag tacctatctg tgaattgcat 600 gcatcttgaa atgcactgga tgtctatcac caagaaatac atcgcagtgt aagattgctt 660 gccactgatg gaccaccata ttttgagtat tgcatttgtt tttctttttt cttttttttt 720 tttttttttt taaaataatt tgcgccgttt tctcgctcat taccagtttt atcgaattgc 780 tctccagcaa atcggctatt catttaattg tatttcatcc attcgctcct ccttgaatct 840 cctccaactt caaactaacc atcccctgaa ttcctccact tcaaggaaat agcacattct 900 aacgtgaacg caatccaaca acgactaatg gtgtaataat ttgcaaccta atatattttt 960 tttgtacgtc aatttaagac acattcatcg tcaacagcga aatatataaa atgaagttgc 1020 aacgctcaac cggaaaagtg catccggcag cattagagta tgtggtataa agtgtactat 1080 ttttaaggtt gtgcgtggga tttcaaggta ggcttagata ctactgaact ggaaaaaaaa 1140 aatatgtccc taaatgtacc aaggcaacgt tatttgagct tgttgccatc atagttacaa 1200 aatgaccatc actcttctca cctacagttg tgggcgccca cttgaaaaaa atacgtatta 1260 cacagagtaa ggtaagacaa aacaaggaag ttgtaaaaag tgcgtttcgt tcttagttgc 1320 ataataattc atgaaatttg taattctttt tatagatagt agataggaat acaacactaa 1380 aaaacagtac acttgtccgg ataagttgtc gcgtatatat cattacattg tatggttgct 1440 gttgatttaa tttggatggt acgaagtttg ccggcaagtt gatactgagc agcgccattt 1500 gagattgtat cgtaatcaga ttggtggctg aagcaacagt agaaaaaaga atggtttgat 1560 ttcgcagttg aagagaaaat gtcattttgt tgagaaaaag ccccgcttca aaagtggact 1620 cggctctaga gcaaaaagtt aatcggcaag tttattcgag tagaatagga agaagtagta 1680 agatagaata gatactagag gagaatggat atgacaataa aatgggttgg tagaaattta 1740 ataagttacg cttgtaaaac actatagcat ttttaaagat acatatggtc aaagacacat 1800 atgatcagag atatacatat gatcaagagt cacccatata taggatggtc aattttttca 1860 ccaaattgtt tttctccatt tttttcattt actgtctatc caacatcgat tagccgcaat 1920 ga 1922 //