ID SPBC557 standard; DNA; FUN; 9749 BP. XX AC AL132869; XX SV AL132869.1 XX DT 11-NOV-1999 (Rel. 61, Created) DT 11-NOV-1999 (Rel. 61, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c557. XX KW dcd1; isochorismatase; pim1; pim1 GTPase protein; KW pyrazinamidase/nicotinamidase; regulator of chromosome condensation; KW RhoGAP GTPase activating protein; serine/threonine-protein kinase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-9749 RA Saunders D., Harris D., McDougall R.C., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (10-NOV-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; P28745; P28745. DR GOA; Q9USS0; Q9USS0. DR GOA; Q9USS2; Q9USS2. DR SPTREMBL; Q9USS0; Q9USS0. DR SPTREMBL; Q9USS1; Q9USS1. DR SPTREMBL; Q9USS2; Q9USS2. DR SPTREMBL; Q9USS4; Q9USS4. DR SPTREMBL; Q9USS5; Q9USS5. DR SWISS-PROT; P28745; RCC1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c557 is overlapped at the 5' end by cosmid c17F3, EMBL CC entry SPBC17F3, accession number AL031263, and at the 3' end by CC cosmid c365, EMBL entry SPBC365, accession number AL078627. XX FH Key Location/Qualifiers FH FT source 1..9749 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c557" FT /map="IIR" FT misc_feature complement(1..97) FT /note="nominal overlap with cosmid SPBC17F3 S. pombe FT chromosome 2" FT CDS 1..201 FT /codon_start=1 FT /db_xref="SPTREMBL:Q9USS5" FT /label=SPBC557.01 FT /note="SPBC557.01, len:66, SIMILARITY:Caenorhabditis FT elegans., Q23279, cosmid zc395., (548 aa), fasta scores: FT opt: 100, E():3.2, (35.4% identity in 65 aa)" FT /partial FT /gene="SPBC557.01" FT /product="putative RhoGAP GTPase activating protein" FT /protein_id="CAB60668.1" FT /translation="IKRWRSFSRHSSATYSNSPSSNFSNMKSSEVDPGSPPRIKSRSYS FT LSRSSSMKLFHTLDRRRENRM" FT CDS complement(634..1245) FT /db_xref="SPTREMBL:Q9USS4" FT /label=SPBC557.02c FT /note="SPBC557.02c, len:203, LOW SIMILARITY:Dictyostelium FT discoideum, Q94471, orfveg109, (239 aa), fasta scores: opt: FT 140, E():0.12, (26.8% identity in 205 aa)" FT /gene="SPBC557.02c" FT /product="hypothetical protein" FT /protein_id="CAB60669.1" FT /translation="MDKKIADLLQELQSSSNVTSNQLENVDKSSIPLSKARKPSDFELN FT NSIEDAEKIKTYPAALRYIFALNSRHPEKLQKLRKMKAMQERQERNWSLERQRLVQHFE FT SKTKLQEILKPLSSDPKLKQPSNCLNDQTNNDSAQTLDDNRALQEFDEQVVQLTNRMYK FT EQLAILADLKIPLFLSVNNDEELSEDQKRLLQLLQDLLGA" FT CDS join(complement(2532..3137),complement(1343..2356)) FT /db_xref="GOA:P28745" FT /db_xref="SWISS-PROT:P28745" FT /label=pim1 or dcd1 FT /note="SPBC557.03c, len:539" FT /gene="pim1" FT /gene="dcd1" FT /gene="SPBC557.03c" FT /product="pim1 GTPase protein" FT /protein_id="CAB60670.1" FT /translation="MTSNRSTRSSTKREEVSKNGVEKRELDESDVMKNGKKPVKRAKVS FT SLPKPVRVPGSAKRINKIPELPTERLNVYVFGSGSMNELGMGEEEMDVVYRPRLNPILS FT TDKVGVVDLAVGGMHSAALSHDGRVYTWGVNDDYALGRLTKDQKDENGDKVDNDLLEGT FT PSKVEGALSHLRVTKVICSDNLTAAITDNGCCFTWGTFRCSDGVLGYSDSQKRTAEPTQ FT MRLPEICQLATGTDHIIALTTTGKVYTWGNGQQFQLGRRMLERRRLQGLTPQPLALKNI FT ISVGAGSYHSFAIDNKGRVYAWGLNITRQCGIEVEDEEEGAVITKPTLVDALEGYNVKS FT ITGGEHHTLALLEDGRVLAWGRDDRHQLGIPDNALPETVVKDEKGNNYYLSTPTIIPGL FT TNVIQVVCGTHHNLAVTSDGKVYSWGSAENYEVGQGDNDEDVAVPTLVRSKAIKEVAIR FT VAGAGGQFSIIAGIPNASEEPVANGIKSEPENEKKLKTEETSKTDDSPVTDAKPDVTSN FT GEPSTATSESKDSVLEPSSTTA" FT misc_feature complement(1565..1711) FT /note="Match to PF00415 RCC1, Regulator of chromosome FT condensation (RCC1) Score 34.71" FT misc_feature complement(1715..1903) FT /note="Match to PF00415 RCC1, Regulator of chromosome FT condensation (RCC1) Score 28.67" FT misc_feature complement(2081..2230) FT /note="Match to PF00415 RCC1, Regulator of chromosome FT condensation (RCC1) Score 24.41" FT misc_feature complement(2357..2368) FT /note="ctaacatactag, splice branch and acceptor" FT misc_feature complement(2526..2531) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(2565..2762) FT /note="Match to PF00415 RCC1, Regulator of chromosome FT condensation (RCC1) Score 33.61" FT CDS join(4357..4456,4529..4662,4702..7086) FT /db_xref="GOA:Q9USS2" FT /db_xref="SPTREMBL:Q9USS2" FT /label=SPBC557.04 FT /note="SPBC557.04, len:872, SIMILARITY:Schizosaccharomyces FT pombe, CAB54861, probable serine/threonine-protein kinase., FT (650 aa), fasta scores: opt: 1160, E():0, (37.7% identity FT in 607 aa)" FT /gene="SPBC557.04" FT /product="probable serine/threonine-protein kinase" FT /protein_id="CAB60671.1" FT /translation="MSVASFLTGEKLLAGSKFVIGKYNVTIEKYIAEGGFSHVYLVQTN FT SKTDGSPITAVLKRMYSPDENALRFVKTEIETMELLKSNPHVVSYIDSCIFPLEKAGVN FT TGFEILLLMEYCAGGGLIDFMNQRLQTRLSEHEVLKIISDIVQGVASLHYLRPPLIHRD FT LKVENVLLSFNTFKLCDFGSVTEPMHAAENSSEIQALEKSINTFTTYQYRAPEMINLYS FT GLGIDEKSDMWALGVLLYKLCYYTTPFETQGPNAILTASYSFPPFPPYSHSLKNVIIAL FT LQPNPCLRPNIFQLMCEICRLRGTALPFRDIYSGRESSFYDLHNRKIMSLLRQTTTTPF FT AGNQVMPTPQVQGSRPPPPPPMPAPIYNVPNVPTVPTVSPNPFLQPTFSGTSQASAHPS FT IHSQSATFNNTQDVNRQSVSSPKVVSSSSVTGSGASGRKVYTPQSKNPFPISQGVTGTS FT SKFSASEVTEDNGSPNAGLDADNVDVEEVVNQRYPDLNELESQLTSHTNITTSSHHNPR FT EQEIAKLADDAFASFRQTAGTSTANSTHTTGTQDISAFDTNFSEGVGSVRSSFDFPDRP FT VSRRPSSTSRSSVKITGPSSPNARSMAYEQHSFNLDDLSNTNFGSNNSLRHAKSFSKYY FT SPSHKDSNKTSRNTSKEGLPSSPTMLYRTTSNTRGDYIVQRTQQSANPLTNIEPQDMSN FT LSTDINASDVVADSTNSILYPTSTASSVANTIASDVDDFGTFNGLQRTVSLHSERASLD FT KKRPSFHDKNPYFKFSPSKYVDEGLEGESELRERVRSYANRSSSSFHNPDLTDTEIQET FT SFNNDLRPVASIQSAETSRSVSDRPANFPEELNDTLFEQKYPGIVDYEEPNSNAEKNVN FT I" FT misc_feature 4457..4462 FT /note="gtataa, splice donor sequence" FT misc_feature 4512..4528 FT /note="ctaacatttttcattag, splice branch and acceptor" FT misc_feature 4663..4668 FT /note="gtatgt, splice donor sequence" FT misc_feature 4690..4701 FT /note="ctaacttattag, splice branch and acceptor" FT misc_feature 4720..5358 FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 116.89" FT CDS join(7533..8047,8090..8662,8706..8919) FT /db_xref="SPTREMBL:Q9USS1" FT /label=SPBC557.05 FT /note="SPBC557.05, len:433" FT /gene="SPBC557.05" FT /product="hypothetical protein" FT /protein_id="CAB60672.1" FT /translation="MDLWKKGKRFFRFSKEPVITCINTSYAGKEIIFDLIANPQMETKD FT VNLTLDFDAPIFHRKAGIDGLLTLTNKSKVNEIQLISIEVFIIGICKHKFHSSTVFLCL FT GTHIMEGQYIVPEELLDYAKKRGKNRIISIPNHSTVDIPFQVNFPKHVEVGPGRQIHSR FT VKVQYFAYANIIYQSADKLRSRRECQEINVLPSISPSSFLDSPSIMCVSKQPDLKKDRQ FT RKSATRITVSLPRTDWLAGESVVSKIKIENHSKSSIKTLKLCLYRRIIGFDPPSWKTQQ FT MMSHLNLSRKNSEVSKTCKCLQKETIRCNKKSNCYNWNGIAALETYTTECHIQIPDKEA FT TITVGSNFEVDHFLKISVGNKLWTLNRVEIPFKIISANSLSLEQENVFLNLEKLTNKAS FT PHGLPISVRLGNSLSYDALSAARRMRNTRYFLYS" FT misc_feature 8048..8053 FT /note="gtaagt, splice donor sequence" FT misc_feature 8078..8089 FT /note="ctaacagttaag, splice branch and acceptor" FT misc_feature 8663..8668 FT /note="gtaagg, splice donor sequence" FT misc_feature 8693..8705 FT /note="ctaatacttcaag, splice branch and acceptor" FT CDS complement(9153..9749) FT /codon_start=1 FT /db_xref="GOA:Q9USS0" FT /db_xref="SPTREMBL:Q9USS0" FT /label=SPBC557.06c FT /note="SPBC557.06c, len:198, SIMILARITY:Escherichia coli., FT PNCA_ECOLI, pyrazinamidase/nicotinamidase, (213 aa), fasta FT scores: opt: 288, E():9.6e-13, (32.9% identity in 167 aa)" FT /partial FT /gene="SPBC557.06c" FT /product="putative pyrazinamidase/nicotinamidase" FT /protein_id="CAB60673.1" FT /translation="SSGESALEVVPVINRLLENDYKWDTVIATKDVHPKDHLSFTTSHS FT STPKPSGTVVNIEAYGHVYKQTLWNSHCVENTPGCEFPDSLNGDRIEFVIPKGSDRLVE FT SYSGFYDAIGRDNGLKAILDKKGITDVFIAGVATDICVKETALHARHWYNTYIISEAVK FT GSSTESHNQAIKDFRDAKIEVISEKDPILQSVRKV" FT misc_feature complement(9231..9749) FT /note="Pfam match to entry PF00857 Isochorismatase, FT Isochorismatase family," FT misc_feature 9649..9749 FT /note="nominal overlap with cosmid SPBC365 S. pombe FT chromosome 2" XX SQ Sequence 9749 BP; 2949 A; 1920 C; 1768 G; 3112 T; 0 other; attaagcgtt ggcgctcttt tagtcgtcat tctagtgcca cttattccaa tagtccgagt 60 tcaaattttt caaacatgaa aagctccgag gtggatcctg gttcaccccc aagaatcaaa 120 tctcgttcgt attcattgtc tagaagctca tctatgaagc ttttccatac acttgatcgc 180 cggagggaaa atcgtatgta atacttttaa ttgtttttct ctgtttatct agtttaattg 240 tctttgtcct tggttgtatg gcgaagaatc tttttgtaca tatatactta gttgttaccg 300 tccgctcttt tagttttatt gggatttgca tttaggctgg ataccgctac tgtcgtttta 360 tgtttttttc tttgtattca gccattttca ttttagaagt tctacttaac catagtaaga 420 acaaattatc caattcgtta ttgttttttg taattgtttt ttgttttggt agatcaatat 480 atttatctac atagtaaatt tatctgacag ctttcagcta ctttcgtaat tttaaatttt 540 tattaatttg gtgtccatat atctgtacaa agaaactttt atgaaaacaa caacgacatt 600 gtaaccagaa aggatttatc aatctatact ttattaggct ccaaggagat cctgtaacaa 660 ttgtaagagg cgcttctgat cttcagataa ctcttcatca ttattcactg acaaaaacaa 720 tggtatcttc aggtctgcaa ggattgcaag ctgttctttg tacatcctgt ttgtcaattg 780 taccacttgt tcatcaaatt cttgtaaagc cctattgtca tctaaagttt gagcggaatc 840 attgttggtc tggtcattaa gacaattgga cggttgtttc aatttagggt cggaggaaag 900 cggtttcagt atttcttgaa gcttagtttt cgactcaaaa tgttgaacta gccgctgccg 960 ttccagagac cagttgcgct cttgacgttc ctgcatagcc ttcatctttc ttaatttttg 1020 taacttttcg ggatggcggg aatttagggc aaagatgtac ctcaaggcag cgggataggt 1080 tttaattttt tctgcgtcct ctatagaatt gttcaactca aaatcagagg gtttccgagc 1140 cttcgataga gggatggatg atttgtctac attttcaagc tgattactcg ttacgttgga 1200 ggagctttga agctcttgaa gcaaatctgc gattttttta tccatttgga aaactcatga 1260 attttgtatg catcaaacac attgatattc aaaataattt atctagccca aaaatagtta 1320 aaagaaattg agaaattaag tactaagcag tggtggagct gggttcgaga acagaatcct 1380 tagattcaga tgtagcggta gatggttcgc cgttagaggt gacatcgggc tttgcatcag 1440 taacgggaga gtcatccgtt ttggaagttt cttcggtttt taattttttt tcattttcgg 1500 gttcgctttt tatgccattt gcaactggct cttcggaagc gttgggaata ccagcaatga 1560 tggaaaattg accacctgca cccgctacac ggatcgccac ttccttgatt gccttggaac 1620 gaactaatgt aggaacagct acatcttcat cattatcacc ttgaccaacc tcgtagtttt 1680 cagcagatcc ccatgaataa accttaccat ctgaggtaac agccaagttg tgatgggtac 1740 cacacacgac ctgtataaca ttagtaagtc ctgggattat agtaggggtt gacaagtagt 1800 aattgtttcc cttttcatct ttaactactg tttctggaag tgcattatcc ggaattccta 1860 attgatgacg atcatctcga ccccaggcta acactctccc atcttcaagt aaggctaggg 1920 tatgatgttc accacctgta atagacttaa cattatatcc ttctaaagca tcaacgagtg 1980 taggctttgt aataacggcg ccttcttctt catcttctac ctctattcca cattgtctgg 2040 ttatatttaa gccccaagca taaacgcgtc ctttgttgtc aatcgcaaaa gaatgatacg 2100 atcctgctcc aactgaaata atatttttta aagctaacgg ttgaggagtc aagccttgaa 2160 gtcgacgacg ttctaacata cgtctaccca gttgaaactg ttgaccatta ccccatgtat 2220 atacttttcc tgtggtagtc aaggcaataa tatggtccgt ccctgtagct aattgacaaa 2280 tttcgggaag tcgcatctgc gtcggttcag ctgtacgttt ttgagaatcg ctatacccta 2340 atacaccatc ggaacactag tatgttagca aacgaaaaat gataaattta taaagccatt 2400 tgttccaaaa gggcgaaggg aaatgataca atgagtcaat agactataaa caacactgca 2460 acgataacag cacctgaatt tggaatcaaa gatcaaatga taatttttca cggccaaaac 2520 aaattactta cacggaaagt accccaagta aagcagcagc cattgtccgt tattgctgca 2580 gtgagattgt cgctacatat aacttttgta acgcgtagat gagataaagc accttcgacc 2640 ttagaaggag ttccctctag taaatcattg tcaactttgt ctccattctc atccttttga 2700 tccttagtaa gacgaccaag ggcatagtca tcattaactc cccaggtgta tacgcgaccg 2760 tcatgagaaa gggctgcaga atgcatgccg ccaacagcca aatcgactac tcctacttta 2820 tctgtagata aaataggatt caaacgagga cgataaacga catccatttc ttcttcgccc 2880 atacctagct cgttcatgga accgcttcca aagacgtaaa cgttcaatcg ttccgtaggt 2940 agttcaggta tcttattaat tcgctttgcg cttcctggga cacgaacggg tttgggtaga 3000 gagctgacct ttgcacgttt aaccggcttt ttgccatttt tcatcacatc tgattcatct 3060 aattctcttt tttcaacccc attttttgag acctcctctc ttttagttga gcttctagta 3120 cttctattag aagtcattat gagttttagc tggaagggtt agctttgcta ctaacaatcg 3180 atttgtaatt taaaaaagaa caagatgtca tcgcgtattc catgcaacca tcaccagacg 3240 caaatccaag aattacattt actttttggt accacttact atagcttggg tttaaaatat 3300 tacaaaagac aaaaaacaac gatttatgtc gtttttgttt tcgcagagac gttgttttca 3360 aaactcgaac aagtcgttct gtatacggat tctcactatt cagtatactg cgaatcgttg 3420 ttatgtgcag cttgtaatta agcattcgtt aattacaaca taagcttcgt ttagcaaaat 3480 aagttattga cttcagtaaa agttcccagc ttgtatttgt tcaaatgtgt gtaaggtgag 3540 ctacgaattt ttatgaaatc ctttcttttt ttttaacttc atttgattta tataaattcg 3600 tttaatcacg tcgaatgttg aaaatttgag acctcttctt atctgagaaa gcggtttcat 3660 gtatagctaa acaaagatca aatttaaaaa aaaagctcat tagtcataag aagttacagt 3720 aagactcgga gtgtccatcg ttcagcaaga tcgtatgcga gaatacgaac tcaagcaacg 3780 cgattttaat ctcttcataa attattttta tgaggaccat atactttttt ccctcatgat 3840 ttaatattta gttacaatga acatagtcgt tagcagtttc tgttttcttt attcttttct 3900 ggatttatat gtctgtgaat ttgccatgtc ataaatttgg gtaacgatgt tactcaactt 3960 gattctacaa gtttgcctgt gaacttgagt atactgtaaa attaatttct cttcttaatc 4020 agctcaaatt attaaaagtt gtagttgaaa ttttcaaaat acggcaagtt gtcttgtggg 4080 gggcggtttt atctatcggt aatggctcaa aggttaaccc tatagaaggg acttgtggtg 4140 tttggtgact ggctcagtac cttgccaaca gcagaattgg catcccttgc tcatcttgat 4200 caaaactgtg taaatacagc cttttcaaag agtaagttat tataataaat ttacaactaa 4260 cgtagatagg gcttcggatg ctgtcccatt gaattgcgag agtgtaaaca ataacctcgc 4320 gacttcagcc agtattgaac aaaaaacctc tctaggatgt ctgttgcatc ctttctcacg 4380 ggcgaaaagc ttctagcagg atccaagttt gtaattggaa aatacaatgt tactatcgaa 4440 aagtacatag ccgaaggtat aatttttggg ttggatggat cgaggagtat tacaatgaaa 4500 gatttatttt actaacattt ttcattaggc ggcttttctc atgtatatct tgttcaaaca 4560 aattccaaaa ctgatggatc accgataact gctgttttga aacgcatgta ttctccagac 4620 gagaatgccc tgcgtttcgt gaaaactgaa atcgaaacga tggtatgtta acgttattga 4680 tttttttgtc taacttatta ggaactactc aaatccaatc cccacgtggt ttcgtatatc 4740 gactcctgca tatttcctct cgagaaagcg ggtgtcaaca ctggatttga aatcctgctt 4800 ttgatggaat actgcgctgg gggtggattg atagatttta tgaatcaaag gttacaaact 4860 cggttgtcag agcatgaagt cttaaaaatt atttcagata ttgtacaggg tgttgcttcc 4920 ttacactatc tgagacctcc tctcattcat cgcgatctga aagtagaaaa tgttttgcta 4980 agctttaata cttttaaatt gtgcgatttt ggaagtgtga ctgagccaat gcatgctgct 5040 gaaaactcta gcgaaattca agcattagaa aaaagtatca acacatttac tacctaccaa 5100 tatcgtgccc ctgagatgat taatttgtat tcaggcttag gaattgatga gaaaagtgac 5160 atgtgggctt taggagtctt gttatacaaa ctctgttact atacaactcc ttttgaaaca 5220 caaggtccta acgctatatt aacagcttca tattccttcc ccccatttcc tccttattca 5280 cattctctta aaaatgtcat catcgcttta cttcagccga atccttgtct tcgtccaaat 5340 atttttcagt tgatgtgtga gatatgtcgt cttagaggga cggctttacc ttttagggat 5400 atatacagtg gaagagagtc gtctttttat gatttgcata accggaaaat aatgtctttg 5460 cttcgacaaa caacaacaac accgtttgct ggaaaccaag tcatgccgac tccacaggtt 5520 cagggctcaa gacctccccc tcctcctcct atgccagctc ctatctataa cgtaccaaat 5580 gtgccgactg taccaaccgt ttcccctaat ccatttttgc aacctacttt ctctggcact 5640 tctcaagcta gtgcacatcc ttctatccac agccaatccg caacatttaa caacactcaa 5700 gatgtgaaca gacagagtgt ttcgtctccg aaggttgttt catcaagttc tgtaactggc 5760 tcaggagcta gtggtcgcaa agtctacaca cctcaaagta agaatccctt cccaatatct 5820 cagggagtca cgggaacatc ttcaaaattt tctgctagcg aggttacaga agataatggt 5880 tcaccaaacg ccggactcga tgccgataac gttgacgttg aggaagttgt taaccagcgt 5940 tatcctgatc taaatgagtt ggagtcacag cttactagtc atactaatat tacgactagt 6000 tctcatcata accctaggga acaagagata gcaaaattgg ctgatgatgc attcgctagt 6060 ttccgtcaga cagctggtac ttcaaccgca aatagtactc ataccactgg tactcaggat 6120 atatctgcct ttgatacaaa tttttcggaa ggggttggta gcgtaaggtc ttcttttgat 6180 ttccctgatc gtccagtatc tagacgacct tcaagcactt ctaggtcgtc tgttaaaatt 6240 actggacctt cgagccccaa tgcgcgttct atggcttatg agcaacactc ctttaacctt 6300 gatgatttga gtaatactaa ttttggttct aataattctc ttagacatgc caaatcattt 6360 tccaaatatt attcaccttc tcataaagat tcgaacaaaa cttccagaaa cacatctaaa 6420 gaggggctcc cctccagtcc tactatgctc tatcgtacaa catctaatac tagaggtgac 6480 tatatcgttc aacgcacaca acagtctgcc aatcctttga caaatatcga accccaagat 6540 atgtcaaacc ttagtactga tattaatgct tctgacgttg ttgccgattc aaccaactct 6600 attctttatc ctacttcgac cgcgtcttct gttgccaaca ctattgcctc cgatgttgac 6660 gactttggaa cttttaatgg tttacaaaga accgtgagtt tacatagtga gcgggcaagt 6720 cttgacaaga agcgtccttc ttttcatgac aaaaatccgt actttaaatt ttctccaagc 6780 aagtatgttg acgaaggatt ggaaggtgaa agtgaattaa gagagcgtgt acgaagttat 6840 gctaaccgct caagttcaag tttccataat ccagatttaa cagacacaga aattcaagag 6900 acgtctttta ataacgattt gaggcctgta gcttctattc aaagtgcaga aacctccaga 6960 agcgtaagtg atagacctgc aaattttcca gaggaattaa acgacacgtt atttgagcaa 7020 aaatatccgg gaattgttga ttacgaggaa cccaattcga atgcagaaaa gaacgttaat 7080 atttgagtta tcaaccagac tttaccgact ttaaactaat tatatttttt tgcaatgatt 7140 tatttcctta ttatatatat aaatccttca tgcaaattcg gtgttgcctt tgaagagttt 7200 tagatggatg attttttgat atgtaccgct tgatgtcacg gcctgtgctt caagtctttg 7260 gaatttttac tcatgttatt ctctgtatct atcgttgttc actagttctc caggaattgg 7320 agttccctat acataactcg gatacatagt agccaaaatt ctaatatcag tttttcaata 7380 taaattccat tttttggatt attgtaaaag ctttagttat tgacaagtta ttatttattt 7440 tcagcgcaga gttttatgtt gtcaaagctc gttattccaa tcgtggttaa ataatcagaa 7500 caagtaaaca aacaaatacc caactagata acatggattt gtggaagaaa ggaaagcgct 7560 tctttcgatt tagtaaagaa ccagtaatta catgtattaa tactagttat gctggtaaag 7620 aaattatatt tgatttaatt gccaacccgc aaatggaaac taaagatgtc aatcttactt 7680 tggattttga tgcaccaatt ttccaccgaa aagcaggaat agacgggctg ctaacactaa 7740 ctaataagtc aaaagtaaac gaaattcagc tgatcagtat cgaagtcttt ataattggaa 7800 tatgtaaaca caagtttcat tcgtcaacag tcttcctgtg tttaggaact cacatcatgg 7860 aagggcaata cattgtacca gaagaactct tagattatgc taaaaaaagg gggaaaaacc 7920 ggataatcag tattcccaat cattcaacag ttgacattcc atttcaagtc aattttccta 7980 aacacgtcga agtaggacct ggccgacaaa tccattcaag ggtaaaggta cagtattttg 8040 catacgcgta agtaaaagtg acaatgatac acgcaagcta acagttaagc aatataattt 8100 accaaagcgc agacaaattg agaagtcgga gagaatgcca agaaataaat gtactaccca 8160 gcatatcacc atcctcgttt ttagactctc cctccattat gtgcgtaagt aaacaaccag 8220 acttaaaaaa ggaccgtcaa cgtaaatcgg ccacaagaat tactgtgagt ttgccccgaa 8280 cagactggct tgccggtgag tcagtggttt cgaaaattaa aatcgaaaac catagcaaat 8340 cctcaattaa aaccttaaaa ctttgtttgt acagacgtat tattggcttc gatccaccaa 8400 gttggaagac acagcaaatg atgagccatt taaatttaag ccgaaagaat tcagaagtct 8460 ccaaaacatg caaatgtttg caaaaagaaa caatcagatg taataaaaag agtaactgct 8520 ataattggaa tggaatagct gctctagaaa cgtatacaac tgaatgtcat attcaaatac 8580 ctgataaaga ggcaacaata accgtcggct caaattttga agttgaccat ttcttgaaaa 8640 tttctgtcgg gaataaatta tggtaaggat taaaatttaa ttaagatata tactaatact 8700 tcaaggacac ttaatcgagt agaaataccg ttcaaaatta tttcggccaa ttccctttcg 8760 ttagagcaag agaatgtatt tttaaatcta gaaaaactaa cgaataaagc ctcaccacat 8820 gggcttccta tatctgtgcg attaggaaat agtttatcat atgacgcatt gtcagcggct 8880 aggcgtatga gaaatactcg ttatttttta tattcctgaa gcagaaatga agttgataaa 8940 tccaaaggca aatgaatgat tgaacattac atagtttttt tgaagcatcc atttaaactt 9000 accgcgtaca gaaaagtatc tcaagaaata cctttattga gatacaaaat gttatttaat 9060 atcactcaat attccataaa tagagcaaca taaggacata aattaaatat aacttttcgt 9120 gaacataaag ggaaagaata aagaaaagaa ggctatactt tcctcacaga ttgtaaaatg 9180 ggatcctttt cgcttattac ttcaattttg gcatctctaa aatcttttat tgcttgattg 9240 tgactctcag tcgaagatcc tttcacagcc tctgatataa tataggtatt ataccaatgt 9300 cttgcatgca aggcagtttc cttgacacat atatccgtgg ccactcctgc gatgaataca 9360 tccgtaatcc cctttttgtc aaggatggct tttagtccgt tgtctcttcc aatagcatcg 9420 taaaatcctg aataagactc aaccagtcgg tcagagccct tgggaataac gaattcaata 9480 cgatccccat tcaaggaatc tgggaattca caccccggag tgttttcgac acaatgacta 9540 ttccataatg tttgcttgta tacatgacca taagcctcta tattcacaac ggtaccagaa 9600 ggttttggtg tactgctatg agatgtagtg aaagaaaggt gatcctttgg atgaacatct 9660 tttgtagcaa tgacagtatc ccatttataa tcattttcta ataaacggtt gatgactgga 9720 accacttcta gggcagattc tccagaact 9749 //