ID SPBC3F6 standard; DNA; FUN; 17311 BP. XX AC AL022019; XX SV AL022019.1 XX DT 02-MAR-1998 (Rel. 54, Created) DT 21-JAN-2000 (Rel. 62, Last updated, Version 4) XX DE S.pombe chromosome II cosmid c3F6. XX KW 3-beta-hydroxysteroid dehydrogenase; glucosyltransferase; KW GTPase-activating protein; RNA-binding; KW serine/threonine protein phosphatase; thioredoxin reductase; trxb. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-17311 RA Lyne M., Rajandream M.A., Barrell B.G., Churcher C.M.; RT ; RL Submitted (28-FEB-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and XX DR GOA; O43049; O43049. DR GOA; O43050; O43050. DR GOA; O43053; O43053. DR GOA; Q92375; Q92375. DR SPTREMBL; O43049; O43049. DR SPTREMBL; O43050; O43050. DR SPTREMBL; O43051; O43051. DR SPTREMBL; O43052; O43052. DR SWISS-PROT; O43053; ALG6_SCHPO. DR SWISS-PROT; Q92375; TRXB_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 80% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c3F6 is overlapped at the 5' end by cosmid c14F5. XX FH Key Location/Qualifiers FH FT source 1..17311 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c3F6" FT /map="IIR" FT misc_feature 1..103 FT /note="nominal overlap with cosmid SPBC14F5, EM:AL023780 S. FT pombe chromosome 2" FT CDS complement(448..1869) FT /db_xref="GOA:O43049" FT /db_xref="SPTREMBL:O43049" FT /label=SPBC3F6.01c FT /note="SPBC3F6.01c, len:473, SIMILARITY:Neurospora crassa., FT O14428, serine/threonine protein phosphatase ppt1., (479 FT aa), fasta scores: opt: 1918, E():0, (57.7% identity in 473 FT aa)" FT /gene="SPBC3F6.01c" FT /product="serine/threonine protein phosphatase" FT /protein_id="CAA17690.2" FT /translation="MAKEALELKNEANKFLKEGHIVQAIDLYTKAIELDSTNAILYSNR FT SLAHLKSEDYGLAINDASKAIECDPEYAKAYFRRATAHIAIFQPKEAVGDFRKALALAP FT SDPAARKKLRECEQLVKRIRFQEAIHNTEPPSPLANINIEDMDIPSDYDGVILEKQITK FT EFVEDMKERFCQGKKLPLKFAYSILRDLKELLEKTPSLIDIPVKGDETLVICGDTHGQY FT FDLLNIFKLHGPPSPTNKYLFNGDFVDRGSWSTEVAFTLYAYKLLYPDAVFINRGNHET FT DDMNKVYGFEGECRSKYNERTFNIFSETFSLLPLGSLISDSYLVVHGGLFSDDNVTLDQ FT LRNIDRFSKKQPGQSGLMMEMLWTDPQPAPGRGPSKRGVGLQFGPDVSKRFCEANGLKA FT VIRSHEVRDQGYEVEHDGYCITVFSAPNYCDSTGNLGAVIKVKEDMELDFHQFEAVPHP FT NIRPMAYANGLLSGM" FT misc_feature complement(499..1359) FT /note="Match to PF00149 STphosphatase, Ser/Thr protein FT phosphatase Score 350.66" FT misc_feature complement(499..1374) FT /note="Pfam match to entry STphosphatase PF00149, Ser/Thr p FT rotein phosphatase" FT CDS complement(2682..3704) FT /db_xref="GOA:O43050" FT /db_xref="SPTREMBL:O43050" FT /label=SPBC3F6.02c FT /note="SPBC3F6.02c, len:340, SIMILARITY:Saccharomyces FT cerevisiae, YGA1_YEAST, putative 3 beta-hydroxysteroid FT dehydrogenase/delta 5-->4-isomerase(3beta-hsd) [includes: FT 3-beta-hydroxy-delta(5)-steroid dehydrogenase(ec FT 1.1.1.145), (349 aa), fasta scores: opt: 1053, E():0, FT (50.7% identity in 345 aa)" FT /gene="SPBC3F6.02c" FT /product="putative 3 beta-hydroxysteroid FT dehydrogenase/delta 5-->4-isomerase(3beta-hsd) " FT /protein_id="CAA17691.1" FT /translation="MPMNSVLVIGSGFLGGHIIRQLCERENLRIAAFDLFENEKLLHEL FT HGQFTMYTGDLTKQGDIERVFEEFHPRVVIHTASPVHNLARDIYFEVNVDGTANIIKAC FT QKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEKQVLEASSESL FT KTAALRVAGLFGPGDRQLVPGMLSVLKNGQTKFQLGDNLNLFDFTYIENAAYAHLLAMD FT NLLSSNPTANGQVFFITNGQVIYFWDFARAIWAHAGHVPPYIIKFPRPVGMLLATAAEW FT VCYFLKKEPGFTRFRVQFSCANRYFNIQKAEDVLKYHPIVDLEEGIRRTLAWMDTEKKH FT " FT misc_feature complement(2697..3704) FT /note="Match to PF01073 3Beta_HSD, 3-beta hydroxysteroid FT dehydrogenase/isomerase family Score 336.32" FT CDS join(4942,5242..6209) FT /db_xref="GOA:Q92375" FT /db_xref="SWISS-PROT:Q92375" FT /label=trr1 FT /note="SPBC3F6.03, len:322" FT /gene="SPBC3F6.03" FT /product="thioredoxin reductase" FT /gene="trr1" FT /protein_id="CAA17692.1" FT /translation="MTHNKVVIIGSGPAGHTAAIYLARGELKPVMYEGMLANGIAAGGQ FT LTTTTDVENFPGFPDGINGTTLTENFRAQSLRFGTEIITETVSKLDLSSRPFKYWLEGA FT EEEEPHTADSVILATGASARRLHITGEDTYWQAGISACAVCDGAVPIYRNKPLAVVGGG FT DSAAEEAQFLTKYGSKVYVLVRRDKLRASPIMAKRLLANPKVEVLWNTVAEEAQGDGKL FT LNNLRIKNTNTNEVSDLQVNGLFYAIGHIPATKLVAEQIELDEAGYIKTINGTPRTSIP FT GFFAAGDVQDKVFRQAITSAGSGCQAALLAMHYLEELEDTD" FT misc_feature 4943..4948 FT /note="gtaagt, splice donor sequence" FT misc_feature 5220..5241 FT /note="ttaattttactgacttcattag, splice branch and acceptor" FT misc_feature 5664..5732 FT /note="PS00573 Pyridine nucleotide-disulphide oxidoreductas FT es class-II active site" FT CDS join(complement(8983..9132),complement(6598..8931)) FT /db_xref="SPTREMBL:O43051" FT /label=SPBC3F6.04c FT /note="SPBC3F6.04c, len:827, SIMILARITY:Saccharomyces FT cerevisiae, Q99207, orf ydl148c., (810 aa), fasta scores: FT opt: 654, E():1.4e-29, (31.0% identity in 848 aa)" FT /note="SPBC3F6.04c, len:827, SIMILARITY:Saccharomyces FT cerevisiae, Q99207, orf ydl148c., (810 aa), fasta scores: FT opt: 654, E():1.9e-29, (31.0% identity in 848 aa)" FT /gene="SPBC3F6.04c" FT /product="conserved hypothetical protein" FT /protein_id="CAA17693.1" FT /translation="MGKNGSQLKNLKSSIRQANLGTRPNNKKSRTRSTESHEDRQAKVQ FT KIQSDFNLFDRQFTKRKFDVGGRRVKGTEGKPGVSRGVGEELRRRTIGAELKKRNRSGA FT IIDRRFGENNPHLSVEEKMLERFSREQQRRSKRELYNLDAEDVLTHGNRPLSDIDSFEE FT PGFGLDEGEELNDEVVRRMHFGGFEDSDAENEKEGEGAHKSKREVMSEIIAKSKHYKAE FT RQAEKERYEDEREKLDEQMEDLQSFLSDYKKASRKSGIKTQRPIISDGDARYDSFVREM FT VFDKRAHPTERTKTEEELAQIEADRLRELEDQRISRMEHYQEDSASEAGSIEDEQATDN FT VFGFGKGLEQENEEEWNGINEEAEESEDEESVNSDTSFVDDEQLKVEEQPLVGSAIKNE FT GSEKASLAYTYPCPTSHVEFVQLLKGLDYKDYPTVVSRIRTLHHVKLHPDNKSRLENFS FT VILLQHILHLTRQPMISMELLEHLTEHLHSLAQQFPSALGISFISVVEGMRKRLAKSYV FT YPEIKFPEISDLLFFNLTGSIFPTSDKKHIVVSPVMLTMAESLSQSPADSLSDVCKKLY FT IANLFLKFQSYSHRYVPEVITAVSQALYLLYPNFISIVPGTFALPDSLKEKQNLFAIQD FT ISLDEPQRLSLYELEELPTGLLQSSILFITLNLIEMAIDIYFKEQAFIEIFVPIMDMLQ FT LYSLKKELLSKRLSEKLLSTLQAVSDSIESAKANRKPLALQSHRPLGITSQVPKFEEGY FT SLDKSSHDIDPERAQLNKLRAQHRDAKKGAIRTLRKDARFIARERRQEQRAKDQAYNEK FT MRKLENRLQHFDPAV" FT misc_feature complement(8932..8946) FT /note="ctaattatttttaag, splice branch and acceptor" FT misc_feature complement(8977..8982) FT /note="gtatgt, splice donor sequence" FT CDS 11270..14722 FT /db_xref="SPTREMBL:O43052" FT /label=SPBC3F6.05 FT /note="SPBC3F6.05, len:1150, SIMILARITY:Saccharomyces FT cerevisiae, LRG1_YEAST, lrg1 protein., (1017 aa), fasta FT scores: opt: 659, E():5.8e-33, (32.3% identity in 1025 aa)" FT /gene="SPBC3F6.05" FT /product="lim and Rho-gap domain containing protein" FT /protein_id="CAA17694.1" FT /translation="MSQRDAKKDGLLYTTNGVSPTPPKRIPVPSRQNKIEENSTTKNFP FT HSRHTSTVAGTEGGSSLSRRHTSAESRKALPNQQQLAQSGLLNKEEQQSLKRSDTSVFP FT KAVRKVSSSKICASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNK FT QVPLCETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEY FT EGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPCHMIYKFWNVKLCQK FT TSIETSKKKDSELSQSQLRKREKHLEQKIFHIWHALSYFEEYTASCISDMLLLVSNGEF FT TKSVICAQKFIRYIEILFKGIDSLETILSSFHAKSMPYVREAKLLCKKLVSIFALLAKC FT HNSDIRDVAIVQDFLSLFTGLAHYLKLLIRISLTGGLRLEQDHACKHALPQFLQTVEEA FT RFVDQEGYDSSSFDMPLNLANASSDLCYVCHSALEEDCVLLGEIRCHIGCLSCTKCKYN FT NRENYDWARWNNQTKQIECYLCYTESSNVSNDEPHPSFEYVSRLSQYIYLLRIALVRLY FT TILMENNDSSQRKPLSVDPKQENVSSTVETAKQAETTASSDSFRKYANTLNDLRHLKSS FT RNRKATSNETQSSSNSTETSKLSKNVSESGKDKSPHWHSHGGSITGKSIVEQASSPLER FT RMDAFDENRAFTLDDIPKVISEQRNREHRPNAFRHMPSYTDPSYRKNSGAIYDKNDGTQ FT KGLTPKSEDAPIRYLSDLSNLELLFTKHVAVLILHPLVRDYYSLDELMEMSDLRKGGFW FT EKFGKAFKGKDAEKKNVKKKGTFGVPLEILVERNNAQSTVGTGVGVKHIPAFIGNTLAA FT MKRKDMSVVGVFRKNGNIRRLKELSDMLDVSPDSIDYEQETPIQLAALLKKFLRELPDP FT LLTFKLFGLFITSSKLESEEERMRVLHLTICLLPKGHRDTMEVIFGFLYWVASFSHIDD FT EVGSKMDIHNLATVITPNILYSKSNDPVDESFLAIEAVHLLIENFEKFCEVPREISLLL FT DDPTLFYNNAAWTSKELYKRCEEIISQMSLDERNTPKHTASTKRKRQPIRRVTTNLTSD FT VPSGSEVADTNSLSSTTKDEASPNSDAQPKPQVKPQHAVIRDS" FT misc_feature 11615..11716 FT /note="PS00478 LIM domain signature" FT misc_feature 11615..11794 FT /note="Match to PF00412 LIM, LIM domain containing proteins FT Score 53.81" FT misc_feature 11615..11800 FT /note="Pfam match to entry LIM PF00412, LIM domain containi FT ng proteins" FT misc_feature 11807..11908 FT /note="PS00478 LIM domain signature" FT misc_feature 11807..11980 FT /note="Match to PF00412 LIM, LIM domain containing proteins FT Score 76.78" FT misc_feature 12722..12739 FT /note="PS00190 Cytochrome c family heme-binding site signat FT ure" FT misc_feature 13295..13318 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 13880..14314 FT /note="Match to PF00620 RhoGAP, RhoGAP domain Score 154.37" FT CDS join(complement(15482..16677),complement(15310..15433), FT complement(15051..15251)) FT /db_xref="GOA:O43053" FT /db_xref="SWISS-PROT:O43053" FT /label=SPBC3F6.06c FT /note="SPBC3F6.06c, len:506, SIMILARITY:Saccharomyces FT cerevisiae, ALG6_YEAST, glucosyltransferase alg6, (544 aa), FT fasta scores: opt: 1380, E():0, (43.9% identity in 526 aa)" FT /note="SPBC3F6.07c initially annotated as hypothetical FT protein but no Met or homology in adjoining cosmid" FT /gene="SPBC3F6.06c" FT /product="glucosyltransferase" FT /gene="SPBC342.01c" FT /protein_id="CAA17695.1" FT /translation="MLSEFENGAPVQQFVSRFRSYSSKFLFFPCLIMSLVFMQWLISIG FT PYSGYNTPPMYGDFEAQRHWMELTLHTPVSQWYFRDLQWWGLDYPPLTAYVSWFFGIIG FT HYFFNPEWFADVTSRGFESLELKLFMRSTVIASHLLILVPPLMFYSKWWSRRIPNFVDR FT NASLIMVLFQPALLLIDHGHFQYNCVMLGLVMYAIANLLKNQYVAATFFFCLALTFKQM FT ALYFAPPIFFYLLGTCVKPKIRFSRFILLSVTVVFTFSLILFPWIYMDYKTLLPQILHR FT VFPFARGLWEDKVANFWCTLNTVFKIREVFTLHQLQVISLIFTLISILPSCVILFLYPR FT KRLLALGFASASWGFFLFSFQVHEKSVLLPLLPTSILLCHGNITTKPWIALANNLAVFS FT LWPLLKKDGLGLQYFTLVLMWNWIGDMVVFSKNVLFRFIQLSFYVGMIVILGIDLFIPP FT PSRYPDLWVILNVTLSFAGFFTIYLWTLGRLLHISSKLSTDLRNKKEA" FT misc_feature complement(15252..15265) FT /note="ttaactggatgcag, splice branch and acceptor" FT misc_feature complement(15304..15309) FT /note="gtaaga, splice donor sequence" FT misc_feature complement(15434..15449) FT /note="ctaatatttttattag, splice branch and acceptor" FT misc_feature complement(15476..15481) FT /note="gtaaaa, splice donor sequence" FT misc_feature 16003..17311 FT /note="nominal overlap with cosmid SPBC342, EM:AL096809 S. FT pombe chromosome 2" FT misc_feature complement(17111..17311) FT /note="SPBC3F6.07c initially annotated as hypothetical FT protein but no Met or homology in adjoining cosmid" FT misc_feature complement(17264..17287) FT /note="PS00030 Eukaryotic putative RNA-binding region RNP-1 FT signature" XX SQ Sequence 17311 BP; 5549 A; 3212 C; 3113 G; 5437 T; 0 other; tgtttaaagt agaagacaca ttgatgactt atgatggcag tgaattttga gggtaataat 60 aataagaaga cagcatttta aatcagtgcg atattaaagg atcaattgtt ttatttactt 120 cattgttcat atgaacttaa acattattta tatttttatt gtacaaagaa cgtgaaatgg 180 aacttttgga ataaagagta gctaatcagg aattaacatt tgggaattcc taaccatcca 240 ggtatcgaaa ccaatcctta agctaaagat taaacataca aatagcatta tttttttgaa 300 cgacggtaac ttcataaaaa cccaaacaac caagtcttca aaaaataatt taactggatc 360 atatagctgt ggaacaaatt ttttcaaaaa ccagaaaatt aaaaaaatgt gggtgcaaac 420 actgagaaca gtgcttgcag tctcccatca cataccactt agcagtccat tggcatatgc 480 cattgggcga atgttaggat ggggaacggc ttcaaattgg tggaaatcca actccatgtc 540 ttccttaact ttaattacgg caccaagatt tccggtacta tcacaataat taggagcact 600 aaacacagta atacaataac catcgtgctc aacttcgtag ccttgatctc tgacttcatg 660 agaacggatc actgctttaa gaccattagc ctcgcaaaaa cgttttgaaa catcagggcc 720 gaattgtaaa cccaccccac gtttcgatgg tcctcgtcct ggagcaggtt gtggatcggt 780 ccaaagcatt tccatcatta acccagattg tccaggttgc tttttactaa atctatcaat 840 gtttcgtagt tggtctaatg ttacgttatc atcagaaaat aggccaccat gtacaacaag 900 ataagaatcg gaaattaggg aaccaagagg tagcagagaa aaagtttccg agaaaatatt 960 aaatgtcctc tcattatact tactacgaca ttcaccttcg aatccgtaga ctttgttcat 1020 atcgtcagtt tcgtgattac cgcgattgat gaaaacagcg tccggatata ataacttata 1080 tgcataaaga gtaaaagcga cttcagtgga ccacgaacca cgatcgacaa agtcaccatt 1140 gaataaatat ttgttagtag ggcttggagg accatgaagt ttgaaaatgt ttaacaagtc 1200 aaaatactgg ccatgggtgt caccgcagat aaccaatgtc tcatcccctt taacaggaat 1260 gtcaattaaa gaaggagttt tttcaagtaa ttcttttaag tctcgcaata tgctatatgc 1320 aaattttagg gggagttttt taccttggca aaagcgttcc ttcatgtctt caacaaactc 1380 tttcgttatt tgcttttcta atattacacc atcatagtca gaaggaatgt ccatgtcttc 1440 aatgttgata ttcgcaagtg gtgaaggagg ttcagtatta tgaatagcct cctgaaaacg 1500 aatccttttg accaattgct cacattcacg aagcttttta cgagcagctg gatcactggg 1560 tgctagtgcc aatgcctttc ggaagtcacc gacagcctct ttgggttgaa aaatagcaat 1620 atgagcagta gctcgacgaa aatatgcctt tgcatattcg gggtcgcatt caatagcttt 1680 ggaagcatca ttgattgcca gaccataatc ctctgacttt aaatgtgcaa gagatctatt 1740 ggagtaaaga attgcgtttg tagaatcaag ctcaatggct tttgtataca aatctatagc 1800 ttgaacgata tgtccctctt ttagaaactt gttggcttca ttttttaatt ccaatgcttc 1860 tttagccatt ttattgagca aaaagtacaa attcaagccc ttgtgagttt ttgtttggtg 1920 gttgatagta gctaaaaaag tttttatctg tccgaacaat gcagcttaat aattggtagt 1980 attgggctac aaagaaacag atatttagga ttctcaaaga tcaaaatgat atttttagaa 2040 caacaaatta atggctttat aaatgcaaac tttgcaacgc agcatacata ttaaaaaata 2100 aatacagaat ttacacaatg ttttacatta gaaatctcaa aaacttacaa gcgtaataaa 2160 agcagtttct atgaagtgtg tctattcatt tatctacgaa ataaaatgac tcgaaactta 2220 taatacatca atacatctca gatgagaaaa acttacaagg caatcattat gtaaaggaaa 2280 taaccattgt tttctaaaca caagtgatac cggacaatct tcgtatagtt caaagaaaga 2340 taacaaaagc aaacgagctg tatccttctc gtctcaagca caacgagtag aaagtacaaa 2400 gagaacgtta tattgatgtt attcgatctt gtctatattc caagataccc aaatattttt 2460 ttttcctttt ttaagatctg agcaatcatc tatcaaatga aatgatgata taagtcatta 2520 acaatttcca aagcggaaaa aaactgtttc gtatcgacat gctcaagagg tagaactcat 2580 cgagaagaaa gtgaactatg tagaaatgaa aaaagactac gaagcgttag aatttaaaaa 2640 ttaaatattc ttttaaagaa taacaaggcc aatggctcaa tttaatgttt tttctcagta 2700 tccatccaag ccaaagtacg acgaattcct tcctccaaat ccacaatggg atggtacttc 2760 aaaacatctt ctgccttttg aatgttaaag taacggttag cacaactaaa ttggacacgg 2820 aaacgtgtaa agccaggttc ctttttcaag aaataacaaa cccattcagc agcagtggct 2880 aagagcatac ccaccggtct agggaatttg atgatataag ggggaacgtg tcctgcatgt 2940 gcccaaatag cacgtgcaaa gtcccaaaag taaataacct gtccattggt aatgaaaaag 3000 acttggccat tggcagtagg attactagaa agtaaattgt ccatagccag aagatgagca 3060 taggcagcgt tttcgatgta ggtaaaatca aaaagattca aattatcacc caattgaaat 3120 ttggtttgtc cattctttaa aacagaaagc ataccaggga cgagttggcg atcacctgga 3180 ccaaataaac cagcaactct aagggcagca gtttttaaag attcagaact tgcctccaag 3240 acttgttttt ctgccaaagc tttggactcg ttataggcat ccatatgaac ttctgggatg 3300 ggctgggact catccacatt gataaggtca gcaccattga aaactacacc tgcggaacta 3360 gtataaacaa gggcatcaac attaaatttc tggcatgctt tgatgatatt cgctgtgcca 3420 tctacgttca cttcaaagta aatatcacga gcaaggttat gcacaggtga agcagtgtga 3480 ataactacgc gtggatgaaa ttcctcaaaa acacgctcaa tatcaccttg tttcgttaaa 3540 tcacccgtat acatggtgaa ttgaccatga agctcgtgta gcaacttttc attttcaaag 3600 agatcaaatg cagcaattcg aagattttca cgttcgcaca gctgccgtat gatatgacct 3660 cctaaaaatc cacttccaat aactaacacg gaattcatcg gcattaaaaa aatacaagaa 3720 taaacgattg tctggttctt gccaggtatt cgacgttaca ccatatcgag aaatgagtga 3780 gcttatttgc tacctgtgtt tttggggtgt aggctagatg caatacacta accatgatta 3840 gcgagaaaga gagcagctgt tgaatatgga acatgggtta gaggatagta gccgattgaa 3900 actctattag tacaatatgt agtacgcact tagaaaacag aaaaattaag tgagtaacat 3960 ctcgagtacc attacaagaa aacgatgacc tcccgacctc gtaccgttca catctaaaac 4020 tggcctattg taaagcaatg ctgcgtgcta ccttaaagct cgataatgtt gatagcagcc 4080 aaaacccaat tttcgtactt tcttcgttga acgttaaacc aatttattat ttataaattc 4140 tatcgttata ccttttatca aatcacaata ttcagcgtaa aacatttttc ctcgttggta 4200 accttaataa ataaattatt aagcagtgtt gatcgcagga acagcaaatt tttctttttt 4260 gggttgtaaa cgactaaaat ttgtttcctt gaaattactt ttcgcaattg ttttttctag 4320 gctacaccaa tcgcacttga atcatacttg tttttggtta aaaccttagt aaggcaagta 4380 gcccagcacg gctacattga gaattttgct ttctgcgcgt ctctttgaca atttgtttgg 4440 attgtggagg cttaaactct taactaacaa aaacgtggat ttttcttgtc gagttctttc 4500 tattgtacta gcataggtca gtcgtctttc atgtacaaag cgtcgcttac tcatggcttt 4560 ggtggttcgt ccatttatcg tggcctcact aattctcgac attcctatac cctcttctac 4620 catgttggca ttaaatttgc ttacgcgata tcggtgtctc gttctgccac tgttaatcta 4680 agcgttcgca cttttacgaa tgtctcgaac gagtctcatt attcatcgct agtaagcaca 4740 ccatttccca gggctctata taagaatcaa tttacccact accaccatat cgcattcctt 4800 cgatacttca ggacattctt tccttatcag aaatttagct tctataaagg catacccgat 4860 tcttttaaga aatctttgaa gttaggatct atttattccc ctttaaaaca aacatatttc 4920 tctacaaata ctcatatcaa tagtaagtga aacgaaaaac gtgcaggaaa tgtgacggcg 4980 atgaagtcga acccaacaaa aaaaaaatta ttacctaaaa ataaaactaa ttcggtgatg 5040 aatttttttc tttttttttg ttgattagta ggaaactttt ttatttccga actcacgtgt 5100 aagattgggt ttcatctgtt tttttatcat tgttcgataa tggttatctt gtgttctttc 5160 agcttttcta tccttctcgc cttacaacat taacgtttat ctttttttgt tttgtttttt 5220 taattttact gacttcatta gtgactcaca acaaggttgt tattattggg tctggtcccg 5280 ccggccatac tgcagccatc tatctcgccc gtggtgagct taagcccgta atgtacgaag 5340 gtatgcttgc caacggtatt gctgctggtg gtcagcttac tactaccacc gatgtcgaaa 5400 actttcctgg ttttcccgat ggtatcaatg gtactaccct tactgaaaac ttccgcgctc 5460 aatctttgcg ttttggaact gaaattatta ccgagactgt ttccaaatta gatctttctt 5520 ctcgcccttt caagtactgg ttggagggtg ctgaagagga agagcctcat accgctgaca 5580 gcgttatttt ggctactggt gcttccgctc gtcgccttca cattactggt gaggatactt 5640 attggcaagc aggtatatct gcttgtgctg tatgtgatgg tgccgttccc atttatcgta 5700 acaagcctct tgctgtcgtt ggtggtggtg actctgctgc cgaagaggcc cagtttttaa 5760 caaagtatgg tagcaaagtt tatgtccttg tgcgccgtga caagcttcgt gcttctccca 5820 ttatggccaa gcgtcttttg gctaacccta aggtggaagt tttgtggaac accgttgctg 5880 aggaggccca aggcgatggc aagttgctca ataaccttcg tatcaagaat accaacacga 5940 atgaagtctc tgatcttcag gttaacggac tcttttatgc tattggacat atccctgcta 6000 ctaagttagt agctgaacaa attgagcttg atgaagccgg ttacattaaa actattaatg 6060 gaactcctcg tacttcaatt cctggctttt ttgccgctgg tgatgttcaa gataaggtct 6120 tccgccaagc catcacttct gctggaagtg gttgtcaagc agctttattg gctatgcatt 6180 accttgaaga attggaagat accgattaga actaatctag aatagttact gattcccttt 6240 tccctttttg gtattcatat gcattttgta tgctcctttc ctttcttttg tttatccaat 6300 aattttagga gtatcttatc aattattgtt tactatagat acatggcttt ttgattcgta 6360 gttattttta cacgaagagc aataataatg attgtttccc tttttctaca ccttagttta 6420 cgcatggtat gtatgtaaaa ttatataagt ttttttacta ttgatataat agtaatagtg 6480 gactttggct ggaagtatta agctaaaatc atcaattacc tataaatttt cccctaatga 6540 tccctatact accctttata atttaaattc aaaaacttaa agaaatgcac actttctcta 6600 gacagccgga tcaaaatgct gcaaacgatt ttccaacttc cgcattttct cattgtaggc 6660 ttggtcctta gcacgttgct cttgccttct ctccctagca ataaacctgg catccttgcg 6720 gagagtgcgg atagctcctt tcttggcatc gcggtgttga gcacgcaact tgttgagttg 6780 agccctttcg ggatcaatat catgggagct tttgtcaaga gaataacctt cctcaaactt 6840 aggaacttga ctagtaattc ctaaaggacg atgagattgt aaagccaatg gtttacggtt 6900 tgctttagct gattcaatag aatcggaaac agcctgtaaa gtagataata atttttccga 6960 taaacgttta ctcaaaagtt cctttttcaa agagtacaat tgtaacatgt ccataatggg 7020 tacaaatatt tcaatgaaag cttgctcctt aaaataaatg tcaatcgcca tttctatcaa 7080 atttaaagtt ataaataaaa tggaagactg tagaagccct gtcggtaact cttctaattc 7140 atataggctt agcctttgag gttcatcgag agatatatct tgaatggcga ataaattttg 7200 cttttccttc aaagaatcag gaagtgcaaa tgttcctggt actatagata tgaaattggg 7260 gtatagcaaa taaagtgcct gtgagactgc cgttataact tcaggaacat aacggtggct 7320 gtaactttga aacttcaaga ataaattggc aatatacagc ttcttgcaaa catctgatag 7380 agaatcagca ggagattggg acaaagactc tgccattgta agcataacag gacttacaac 7440 aatatgcttt ttatctgaag ttgggaagat tgaaccagtc agattgaaaa acaataagtc 7500 agaaatttca ggaaacttaa tttccggata cacataagat tttgctaggc gtttgcgcat 7560 cccttcaaca actgaaataa aggatattcc taaagcactg ggaaactgtt gtgccaaaga 7620 gtgtagatgc tcagtcaaat gctccaataa ctccattgaa atcataggtt gacgagttaa 7680 gtgaagtata tgttgtaata aaataacaga aaaattttca aggcgcgatt tgttatctgg 7740 atgcagctta acatgatgca aagtacgtat acgagagact acagttggat aatccttata 7800 atccaggcct ttaagaagtt gaacaaactc tacgtgagaa gtaggacacg ggtacgtata 7860 tgctaaagac gctttttcag agccttcgtt ttttatagcc gatccaacta aaggttgttc 7920 ctccactttt agctgttcat catctacaaa agatgtgtcc gaatttactg actcttcatc 7980 ttccgattct tcagcttcct catttattcc attccattcc tcctcatttt cctgttctaa 8040 accttttcca aacccaaata cgttatcagt agcttgctca tcttcgatgc taccagcttc 8100 actagcagaa tcttcttggt aatgttccat tctagaaatt ctttggtcct ctaattcacg 8160 caaacgatca gcttcaattt gagcaagctc ctcttcggtc ttagtacgct cagtaggatg 8220 cgcgcgtttg tcgaaaacca tttcacggac aaatgaatca taacgtgcat ctccgtctga 8280 aattattggt cgttgtgttt ttatgccgga cttcctagat gcttttttat aatccgacaa 8340 aaaggattgc aaatcctcca tctgttcgtc aagtttttct ctttcgtcct catatctttc 8400 tttctctgct tgacgttcag ctttgtaatg cttggacttg gcaataattt ccgacatcac 8460 ttcacgttta gatttatgtg caccttcacc ttctttctca ttttcggcat cagaatcctc 8520 aaatccgcca aaatgcatgc gacgaactac ttcatcgttt aactcctctc cttcatcaag 8580 tccaaatcca ggttcttcaa aggaatctat atcagacaaa ggcctattac catgagtcaa 8640 aacatcttct gcatctagat tgtaaagctc tcttttgcta cgacgctgtt gttcacgact 8700 aaatcgttct aacattttct cttcaactga taaatgtgga ttgttctcac caaatcgtcg 8760 atcgattata gctccagaac gattcctctt tttcaattca gccccaattg ttctccttcg 8820 aagttcctct ccaacacccc gactaactcc gggtttaccc tcagttccct ttacacgtct 8880 accaccaaca tcaaatttcc gttttgtaaa ctgcctgtca aataaattaa acttaaaaat 8940 aattagctat tgctctcata agctggaaag acaaaaacat acatctgatt gaattttttg 9000 aactttagcc tgtcggtctt catgggactc agtacttcta gttctcgact ttttattatt 9060 tggacgagta ccaaggtttg cttggcgaat actcgatttc aaatttttta attgtgatcc 9120 atttttaccc ataacagcaa tttgatgaag aaattacctt ctggaagaaa atgtttggta 9180 tatgataaaa agttaatatt gtgagtactc acactaaaat actgtaaact gtgcattatt 9240 atattttggg gcaaaggata aattttataa ttatataaag gtggttcacg aactgtaaac 9300 aaatcaacca cctagtgtcc tgcaacgttc cagatcagta atattatatt caattttaaa 9360 ataagtattt tggtaaattg ttaaagtgct tcttgaaatc atactgttga cttgtgattg 9420 ttaaatggtc aggcgtttcg acgcggtgca tgaaaagcag catcatcaat ctttcattta 9480 aacactttta cgtatttata taaaccatta aaaaagttat gcctacaatt gaattaattc 9540 aaaaggattc cagtagtcca caaacgattg caatatataa cgcgcacgac attttttctt 9600 tgtaagtaat ttaacaacaa taaaagtgtg gttcatttca ttctatgctt cttctttatt 9660 tttctttatt tccttctcat tttttagata aaaaaatttt ctgttgcatt acatgaaata 9720 taaatgctta tgatgattat tcgactttaa tacatttatc aaaggccttt acccacaaat 9780 cttttgtaag taaccgtttc gattaacctc ttggaacaga tatctattgt ttgtttactg 9840 aatttacaac cttcacttgt tattgctcaa accatcacaa ttgatcattt ctgattattt 9900 tcttacataa attaatgctc attttctctt tgatttttct taagtgctaa tcctttaact 9960 tattaaccgc ggtaactcat aaagctgatc tacttcatga attcgtaaac tatttcttct 10020 gagataataa atacccgtag ttttttaacg aaatttgctc atgactcagt gacgaatagt 10080 agttttgaag ctgtaaagga tttgaggtcc attgttagta taataataca caacttttta 10140 cagacgaaag tagcttcaag caacaaatcg aataaggatt cagaattcga atcaattgtg 10200 tggaagtttg tgcaactaag ggtcgtaaaa gaagatgtaa actctgaatt agcacaacat 10260 aggaccttag cgttcagtct gtgaagaagc caaaatgatg caagttaagc aaaacaaaaa 10320 gcagttatga aactactcaa aaatgctcga atgcctttgg tttccttttt ctcataaaaa 10380 aataatcgtt atattatgat aattcagtat agaagcaact gtcagaaata aaaataaaaa 10440 attctgcatg tatttttaag ataatgtatt aagtgatttg agtttttata aactccattt 10500 attttatctg aattcaaccc tactgaactt gcaagacgta cgtagtctgg gaaacacagt 10560 gggaaagtac ttaaatactt tttgggaaac ttgaacaggt agttaaaatc aaagtcactc 10620 atattgtcta tgataatttc aaacatctat aaggactctt gttataacat agacttcgta 10680 aatgtgtaaa agccttagca tgagcagcga gcagctctag tataatagtc gttgtaaaat 10740 tcagtgccta aacctacacc taagaataac tatccaaaag gagcataaag tgtagcatta 10800 aagagagata aaacaaaaat acagattatt ctctaaacga agatctaaag tcattttcgg 10860 ggtgtgtact gaaatcggtt tcccaaatga cttcatgtct tagtcgctat aaaaattagg 10920 gtctattttg gcccctatat caagctggag tttagcattt ctgacttgga ttccatggtg 10980 tgcagtctcc tcttaggtgg aagtcggacc aacaaccacc aacataccgc aacaatagtt 11040 agtctctgaa aaaaaatagc ttcaaaagct ataggcttag ttatttgagg tcttacatat 11100 tattaatttc actcttttaa aaactgaagg aattgagtcc cataaggttt tcttgggggt 11160 gattttcgaa cggaaatatc ccaaagagga ttaactagtc atactggttt attaagtgat 11220 aaaggcgttt aatatagaag ctaaggttcg ttcaaacagc aaggacagta tgtcccaaag 11280 ggacgccaaa aaagatgggt tgctgtacac aacgaatggt gtatcaccaa caccaccgaa 11340 acgaattcct gttccttccc gtcaaaataa gatagaagag aattctacaa cgaagaattt 11400 cccccattct cgacatacaa gcactgttgc tggtactgag ggtggcagct cactttctcg 11460 gaggcatacc tctgctgagt ctcgaaaagc gttgcctaat cagcaacaat tagcccaatc 11520 tggtttgctt aataaagagg agcagcaaag tctaaagcga tcagatactt ctgtatttcc 11580 taaggctgta aggaaagttt cttcatcaaa gatatgtgct tcatgtggac aagttatatc 11640 cggacaatat gtacgtgctc ttggaaatat atatcactta gaatgttttc gatgccatga 11700 ttgtaattct ttagttgctt caaaattttt tcctattgat gatcccactc ttaacaagca 11760 agtgccttta tgtgaaacag attattttcg gcggcttgac ttactttgtg caagttgtgg 11820 aatggccctt cgaggttatt atattacagc tttgaataaa aaatttcata tagaacattt 11880 cacttgttcc ttatgttaca ctgtttttgg tccgaatgat tcttactatg aatatgaggg 11940 caaagtgtat tgtcattatc attattcaac acttttcgct gcacgatgtt gcggttgtga 12000 tggtccaatt ttaaggcaat ttgttgaagt ttacagaaat ggggtatctc aaaattggca 12060 cgttccttgt catatgatct acaagttttg gaatgttaaa ctttgtcaaa agacatccat 12120 agaaacttct aaaaagaagg attctgagct ttctcaatca caattacgca aacgggaaaa 12180 acatttagaa cagaaaatat ttcatatttg gcatgcactc tcttattttg aggagtatac 12240 agcttcgtgt atcagtgata tgcttctcct tgtttcaaat ggagaattca caaaaagtgt 12300 tatttgtgcc caaaaattta ttcgttatat cgagatactt tttaaaggca ttgattcgct 12360 agaaactata ctttcaagtt ttcatgctaa aagtatgcct tatgttcgtg aagctaagtt 12420 gctttgcaag aagctagttt caatatttgc tttacttgct aaatgccata attcggatat 12480 tcgcgacgtt gctattgttc aagacttttt atcactcttt accggtttag ctcattacct 12540 caagttactt attcggatat cgcttacggg aggtttacga ctagaacaag atcatgcttg 12600 taagcatgct cttccccagt ttttacagac cgtagaagaa gctcgatttg tcgatcaaga 12660 aggatatgat agttcttcgt ttgatatgcc tttaaatttg gccaatgcca gctccgattt 12720 atgctatgtg tgtcattctg ccttggagga agactgtgtt ctcctgggtg aaattcgctg 12780 ccatattggc tgtttgtcat gtactaaatg taaatacaac aatcgggaga attacgattg 12840 ggcgcgctgg aacaaccaaa cgaaacaaat agaatgttat ttatgttata ctgaatcatc 12900 caacgttagt aacgacgaac ctcatccgtc ttttgagtat gtgagcaggc tttctcaata 12960 tatttacttg ctacgtattg ccttggttcg attatatact atacttatgg agaataacga 13020 ctcatcacag cgaaagccgt tatctgttga tccaaaacaa gagaatgtat ccagtactgt 13080 tgaaacagcg aaacaagccg agactactgc cagttcagac agttttcgta agtatgccaa 13140 tactcttaat gatttgcgac atcttaagtc ttcccgcaat cgtaaggcca cctcaaatga 13200 aacccagtcc tcaagcaatt ctaccgaaac atctaaatta tcaaagaatg tgtcagagtc 13260 tggaaaagac aaaagtcccc attggcattc tcatggtggt tctattactg gaaaaagcat 13320 agttgagcaa gcatctagtc ctttggaaag aaggatggat gcctttgatg aaaatcgtgc 13380 ttttactctt gatgatattc ctaaagtcat ttcagaacaa agaaacaggg aacaccgacc 13440 aaatgcattt aggcatatgc ccagttatac tgatccttcc tatcgaaaga attctggggc 13500 gatttatgat aaaaatgatg gtacgcaaaa gggtttgact cctaaatcag aagatgctcc 13560 tattcgttat ctctcagatc tttctaattt agaattatta tttaccaagc acgtagctgt 13620 attaatattg caccctcttg tacgggatta ttattcactt gatgaattga tggaaatgag 13680 cgatttgcgc aaaggtggtt tctgggaaaa atttggaaag gctttcaagg ggaaagatgc 13740 cgaaaaaaag aacgtgaaaa agaaaggtac ctttggggtg ccattggaga ttcttgttga 13800 gcgtaataac gctcagtcga cggttggaac aggcgtgggt gtcaagcata ttcctgcctt 13860 tattgggaat acattggctg ctatgaaaag aaaagatatg tcagtagttg gtgtatttag 13920 aaagaacgga aatatacgta ggttaaaaga gctatctgac atgctcgatg tttctccgga 13980 ttcaattgat tatgagcaag aaacacccat tcagttggct gctttattga aaaaattcct 14040 tagagagctt cctgatcctc ttttaacttt caagttattt gggttgttca ttacgtcttc 14100 aaagttggag tctgaggaag aacgcatgag ggttttgcat cttacaatat gtttactacc 14160 caagggacat agagacacta tggaggtaat ttttggcttt ctatattggg ttgcctcttt 14220 ttcccacatc gatgatgagg tgggaagcaa aatggatatt cataatcttg ctaccgtaat 14280 tactccgaac atattatatt ccaaaagcaa cgatcctgtt gacgaaagtt tccttgccat 14340 cgaggctgtt catctgttga ttgagaactt tgagaaattt tgtgaagtcc ctcgagaaat 14400 tagtctttta ttagatgatc ctactctttt ttataataat gcggcttgga catctaaaga 14460 actatataaa aggtgtgaag aaataatttc tcagatgagt cttgacgaac ggaatactcc 14520 aaaacatacg gcgtctacaa agaggaaaag gcaacctata agaagagtta caactaattt 14580 aacgtcagac gtgccttctg gttctgaagt tgctgacaca aattcgcttt catctaccac 14640 taaagatgaa gcttcaccta acagcgatgc tcagcctaaa cctcaagtta aacctcagca 14700 tgcagtcatt agggattcct aatttatcga tacacaatcc catttgtctt tttttgtttt 14760 atcatatttt caatttttac tgaacattag atcagctacg aaagtagaaa gctcggtctg 14820 gtaccgctta caactttgtc gtgttgaatt cgatgggatg atggtacttt aataatcgtt 14880 cttttaataa aaggttactt ggctaaaata atatttcagg caaatataac tttcgtttaa 14940 tatttgaagt catgaataac aatccttctc ccagaattgt tttaataaaa tcctttacta 15000 tatgttgatt tttccgcgta ataatgtaac ataaaatcat tttgttcaat ttatgcttct 15060 tttttgttac gcaaatcagt agaaagttta cttgaaatgt gtagcaagcg accaagagtc 15120 cataagtaaa tagtaaaaaa tccggcaaat gaaagcgtaa cattaagaat gacccataaa 15180 tcggggtaac gggatggtgg tgggataaac agatcaattc ccaaaataac aatcatacca 15240 acataaaaag actgcatcca gttaatacac ctggcataaa aaaatagcaa ataataaaaa 15300 tagtcttacc agttggatga accgaaaaag gacgtttttt gagaaaacta ccatgtcacc 15360 aatccaattc cacattaata ctaatgtgaa gtattgtaaa ccaagaccat cttttttcag 15420 taacggccat aagctaataa aaatattagt ataatttaaa cagtaaatat gtatatttta 15480 cctgaaaaca gcaagattgt ttgctagagc aatccatggt ttggttgtaa tgtttccgtg 15540 acataagaga atagaagtgg gaagtaaagg aagaagaacc gacttttcat gaacttggaa 15600 agagaacaaa aaaaaccccc atgatgcact tgcgaaaccc agagctaaaa gacgtttacg 15660 aggataaaga aataatataa cacaagatgg gagaatagaa atgagagtaa aaatcaagct 15720 aattacttgg agttgatgta aagtaaacac ctctctaatt ttgaaaacag tgttgagggt 15780 gcaccagaaa ttagcaactt tatcttccca aagaccacga gcaaacggga acactcgatg 15840 aagaatttga ggaagcaatg ttttatagtc catgtaaatc catggaaata atattaagga 15900 aaacgtaaaa acaactgtga ctgacaacaa gataaatctt gagaaacgaa tctttggctt 15960 tacacaagtt cccaataagt aaaaaaaaat aggaggagca aaatatagag ccatctgttt 16020 aaacgtaaga gctaaacaga aaaaaaacgt agcagctaca tattggtttt taagcaaatt 16080 agcgattgca tacataacta aacccagcat aacacagttg tactgaaaat gaccatgatc 16140 aattaagagt aaagcaggtt gaaaaaggac cataattagg ctagcatttc tgtccacaaa 16200 attaggtata cggcgcgacc accatttgga gtaaaacatt aaagggggaa cgagtattag 16260 aagatgagat gcaataacag tagatctcat gaataatttt aattcaagac tttcaaatcc 16320 acgagatgtg acatcagcaa accattcggg gttgaaaaaa taatgcccaa ttataccaaa 16380 gaaccaagaa acatatgctg ttaaaggagg ataatctaat ccccaccatt gcaaatctct 16440 aaaataccat tgagacacag gagtatgcaa tgttagttcc atccaatgtc tttgcgcttc 16500 aaaatcgcca tacataggag gtgtattata cccagaataa ggaccgatag atataagcca 16560 ttgcatgaaa actaaactca ttataaggca tggaaaaaat aaaaatttag atgaatagga 16620 tcgaaaacga gaaacgaatt gttgaacggg agccccattc tcaaactcag atagcatgtt 16680 ttaagcggcc gtcaaacggt aaggatttcg ctttaaaact ttgggtttct aaattaaaat 16740 atccaacttg gaaagcttaa agattaaaaa acgagtgaat ttgtatccgg aatgttttaa 16800 acaaggaaaa gctaattcaa agcagataca agttgggata acagtgaaat gggttgggaa 16860 ccacgaagtg gactatgggt ccaccattgc gatcatcgtg aaatatggtt ttataatgtc 16920 caaaaaaatc aattcacttt ttaattcatt ctttctttct tattttttat tttcagtttc 16980 aaagcctaaa aattaaatac gcgcaaagtt atcactacat aattgatcca agggtgagca 17040 ataacataaa aaatgagttg ataaacatga aaattaagct gtatcactta ttgactgatt 17100 tacttttctc ttaagatagg taaatgttag aataaattga gcttggcaag ttaggagttt 17160 gtttcagtga tactagccca ttaaattcaa tgaactgaaa tatattactt aattgtttgc 17220 tgtttaataa tttagctcta acgaaaatct tattagcaaa gaacatgcta agaaaaataa 17280 ttcccttaat acatttacta tgtaattgat c 17311 //