ID SPBC36B7 standard; DNA; FUN; 16169 BP. XX AC AL391743; XX SV AL391743.1 XX DT 31-AUG-2000 (Rel. 64, Created) DT 31-AUG-2000 (Rel. 64, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c36B7. XX KW chromatin decondensation; eif-2alpha kinase; ER protein-translocation; KW FYVE zinc finger; nuclosome assembly factor; KW Phosphatidylinositol(3)-phosphate binding; sec61 alpha subunit; SET domain; KW Syntaxin homolog (t-SNARE). XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-16169 RA Wood V., Harris D.; RT ; RL Unpublished. XX RN [2] RP 1-16169 RA Barrell B.G., Rajandream M.A.; RT ; RL Submitted (29-AUG-2000) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9HGN1; Q9HGN1. DR GOA; Q9HGN2; Q9HGN2. DR GOA; Q9HGN5; Q9HGN5. DR GOA; Q9HGN6; Q9HGN6. DR GOA; Q9HGN7; Q9HGN7. DR SPTREMBL; Q9HGN1; Q9HGN1. DR SPTREMBL; Q9HGN2; Q9HGN2. DR SPTREMBL; Q9HGN3; Q9HGN3. DR SPTREMBL; Q9HGN4; Q9HGN4. DR SPTREMBL; Q9HGN5; Q9HGN5. DR SPTREMBL; Q9HGN6; Q9HGN6. DR SPTREMBL; Q9HGN7; Q9HGN7. DR SPTREMBL; Q9HGN8; Q9HGN8. DR SPTREMBL; Q9HGN9; Q9HGN9. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c36B7 is overlapped at the 5' end by cosmid c19G7, CC EMBL entry SPBC19G7, accession number AL021839. XX FH Key Location/Qualifiers FH FT source 1..16169 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c36B7" FT /map="II" FT misc_feature 1..121 FT /note="nominal overlap with SPBC19G7 S. pombe chromosome 2" FT CDS 1..1013 FT /codon_start=3 FT /db_xref="SPTREMBL:Q9HGN9" FT /label=SPBC36B7.01 FT /note="SPAC36B7.01, SIMILARITY:Yarrowia lipolytica, FT S61A_YARLI, protein transport protein sec61 alpha subunit., FT (471 aa), fasta scores: opt: 671, E():0, (34.2% identity in FT 325 aa)" FT /partial FT /gene="SPBC36B7.01" FT /gene="SPBC19G7.17" FT /product="protein transport protein sec61 alpha subunit FT homolog" FT /protein_id="CAC05722.1" FT /translation="GSFSDLGIFRFIMLILQIFLPGIVCIYLCEIIEKGHGLGSGPVLL FT LGSHILGNIMWDVLSLHRYPVNESGDSQYQGALVGFAFNLFSFKNKFSSLRSILFRSEG FT LSFVQFLVCIAVFATFMYTLNIRIDVPIRSSRVRGVRQNFPLKLLYTSVIPLIYFYSIL FT SHLLVFAYALYSLCPNSLITRLLVQYSPIDTFAEHKLQLVGGLVYFLYPPLGLSEALLH FT PVHTVIYTITLICITIYFSLLWMNATAGGPRDVLLFFKENQLVIAGYREATMLKELEKI FT IPIAAKLSAFFVSILSVIAGIFASTFGVGVLIASALVYASFEMIVGANTSLGNGN" FT CDS 2175..3236 FT /db_xref="SPTREMBL:Q9HGN8" FT /label=SPBC36B7.02 FT /note="SPBC36B7.02, len:354, SIMILARITY:Schizosaccharomyces FT pombe, YC9B_SCHPO, hypothetical 43.0 kda protein c584.11c FT in chromosome iii., (380 aa), fasta scores: opt: 199, FT E():6.2e-06, (21.9% identity in 338 aa)" FT /gene="SPBC36B7.02" FT /product="hypothetical protein similar to S. pombe FT SPCC584.11c" FT /protein_id="CAC05723.1" FT /translation="MSSSYSSRAEKYDLISCKDLTWDLSRSHLTTQYKSFYMCFDDGSF FT GYLQIAYGNLGLLVKVTPLGWTYYPAKTEKNVEPIVNSSTYLYRSMKISKDRYSVDVQK FT HFMHFNSSTREWHIVIEGVYDLKIKTEDSGFSLVDFGQNDSSSRMEHKIFPINTVEGTV FT TAHGREKKLTGVAVYVGALFFREKFTNLGHTWQNSILFDKSKKSILTCLHYFPRDPSQP FT FRSQASLTLDGKLIAVLLDNEYSTKGAAQVDGIRYLLPEQIHRILSGQTFDGKPVRVEL FT SAELEELDSITDVLKIFPKWAKDIVSIWAGLPFVFVWLQHITAEVFIDNEHVATLSGCY FT YLEQTCVHLPQTC" FT CDS join(3829..3943,3994..4084,4145..5774) FT /db_xref="GOA:Q9HGN7" FT /db_xref="SPTREMBL:Q9HGN7" FT /label=SPBC36B7.03 FT /note="SPBC36B7.03, len:612, SIMILARITY:Saccharomyces FT cerevisiae, NPL1_YEAST, npl1 protein, (663 aa), fasta FT scores: opt: 699, E():0, (27.0% identity in 673 aa)" FT /gene="SPBC36B7.03" FT /product="putative component of ER protein-translocation FT subcomplex; DNA J protein" FT /protein_id="CAC05724.1" FT /translation="MSSEYKYDEQGIFFPVFLLVGTSCCVLPLTYSTILGPSASKEKKN FT VRDPFQKYRPKDLKVQRKSIFRLRYIFLILGWLAIGFLSYKIANSRLKLNIWDPYEILG FT IAKGTSVDDVRRHYKRLSIKFHPDKVRNMVNTTREEVEKHYIEITNAYRALTDDKTREN FT YALYGTPDVPQHISVGIALPKWISESENSIYILGFYGLVFGIVLPYAVGKWWYGSRTYT FT RDHVHVDTVDEWFPKMETSLTLDELLSLFASSKELTSLVPNEKNPKEYILKLLFDHLNR FT KKTNNFNTHQILSQSDVVLNALLSVATAFGFANPVDNVLKLWQHIVQAIPLDAPFPLLQ FT LPHLLMEDVKNLSIRNISSIPQFLSLSEEQTKDYLPNYSKNQLKEMREIANGIPRISVV FT AAKVLVDDDEYITVGAIANLILDLKCSYGTEVVPEVSTDGTETATKKDEEDAEKYHQSK FT DVVLGDVETLPYAWAPYFTQHHKTAWWIYVVDPRQNRVIVPPFSITDIPKTLRTFRIPF FT QVPPVAGTFSFQLHIMSNSYVGEDVISNLTMIVKDTSVLQEQLQEEAVSDLEDNSDIDE FT SANAGKDFSDDENIGSDEEDESEYDPMDTDTSDGE" FT misc_feature 3944..3949 FT /note="gtaagt, splice donor sequence" FT misc_feature 3974..3993 FT /note="ctaatatggtgaatttttag, splice branch and acceptor" FT misc_feature 4085..4090 FT /note="gtatga, splice donor sequence" FT misc_feature 4126..4144 FT /note="ttaatttttattattatag, splice branch and acceptor" FT misc_feature 4230..4442 FT /note="Match to PF00226 DnaJ, DnaJ domain Score 69.44" FT CDS 6403..7602 FT /db_xref="GOA:Q9HGN6" FT /db_xref="SPTREMBL:Q9HGN6" FT /label=SPBC36B7.04 FT /note="SPBC36B7.04, len:400, SIMILARITY:Saccharomyces FT cerevisiae, YMI0_YEAST, hypothetical 48.1 kda protein in FT tub1-cpr3 intergenic region., (423 aa), fasta scores: opt: FT 1303, E():0, (50.5% identity in 400 aa)" FT /gene="SPBC36B7.04" FT /product="protein with weak similarity to Azospirillum FT brasilense nifR3 protein; yeast YML080W homolog; UPF0034 FT domain" FT /protein_id="CAC05725.1" FT /translation="MASKKLHGRDFYNKIGRPKRILAPMVDQSELPWRILARRSGADLC FT YSPMFHSRLFGESEDYRNKVFSTRTIPEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVD FT VNLGCPQGIAKKGKYGSFLQENWNLIESIITKLHTELSIPVTAKIRIFPDPQKTLDYAK FT MILKAGASILAVHGRLREQKGHFTGIADWEQIQMLRKNLPSETVLFANGNILHAQDIDR FT CIKYTGVDGVLSAEGSLYNPRIFLPPSSPLMTLYPRIDDMCEEYLNIIREFKLESDYSS FT LSAIKGHLFKLMRPLLSIHTDIRSKLAQGCTPRDFETFPPVVAMLRKRLLECEEKGEIN FT EDKDVKESVKDSMGYPVIPWWRVQPYIRPLEVPLVTKRKPVEVTADEGPVKKKVNASVA FT " FT misc_feature 6463..7350 FT /note="Match to PF01207 UPF0034, Uncharacterized protein FT family UPF0034 Score 248.68" FT CDS complement(7994..8833) FT /db_xref="GOA:Q9HGN5" FT /db_xref="SPTREMBL:Q9HGN5" FT /label=SPBC36B7.05c FT /note="SPBC36B7.05c, len:280, SIMILARITY:Saccharomyces FT cerevisiae, Q06651, similar to proteins involved in FT vacuolar function: s.cerevisiae vac1p., (286 aa), fasta FT scores: opt: 370, E():3e-17, (26.9% identity in 260 aa)" FT /gene="SPBC36B7.05c" FT /product="putative Phosphatidylinositol(3)-phosphate FT binding protein; contains FYVE zinc finger" FT /protein_id="CAC05726.1" FT /translation="MDEDETTYPEALRRLLIETPAAIWQLDDESAQCNNCGGPFTWFRR FT RHHCRWCGKLFCYNCCNSFAKLPVSSVSVDPTEDLIPQDMFIRDPDFLANDNDDDNDSQ FT DSSWINVRVCVNCRQQLSELKELDLPYPITTCLNDDTTTRSNNQVIQSSCGNNLQRIEE FT PDDFVECPVCYAPLSSFKTLSERESHVANCLSNNSSSPRNMTEFLKHARRYISMQLSET FT SPCLGQECIICFEEFAAGDRVARIEYCLCIFHLKCYRDWLSTGAAGCPVHAATLHLS" FT misc_feature complement(8465..8764) FT /note="Match to PF01363 FYVE, FYVE zinc finger Score 77.36" FT CDS join(complement(9706..9839),complement(9287..9662)) FT /db_xref="SPTREMBL:Q9HGN4" FT /label=SPBC36B7.06c FT /note="SPBC36B7.06c, len:170" FT /gene="SPBC36B7.06c" FT /product="hypothetical protein" FT /protein_id="CAC05727.1" FT /translation="MSEIITSLQTELSNRHANMVSEAVLAFSEHHDSALHSYFLNARVK FT DVILESLRTTEEKLRALDKLQLDASKNLSNLLVEYNSINAELESVNSKISSISSQEYAL FT EEFQALSQNFEKNLVQHRRNSQNKLKCAMEVNIKTNYIVILLTSINLKKLKMIETSTHK FT AILDNF" FT misc_feature complement(9663..9679) FT /note="ttgacccaacaattcag, splice branch and acceptor" FT misc_feature complement(9700..9705) FT /note="gtacgt, splice donor sequence" FT CDS join(10410..10438,10530..11178) FT /db_xref="SPTREMBL:Q9HGN3" FT /label=SPBC36B7.07 FT /note="SPBC36B7.07, len:226, SIMILARITY:Saccharomyces FT cerevisiae, Q03322, d8035.11p., (224 aa), fasta scores: FT opt: 349, E():1.4e-14, (30.0% identity in 230 aa)" FT /gene="SPBC36B7.07" FT /product="Syntaxin homolog (t-SNARE)" FT /protein_id="CAC05728.1" FT /translation="MEDPFYEVKADASNQMEQVRKLYNSFMAARNSGVLSPNTELTYAI FT DELSETLKDLKAAVEIAMKNSEHFGLDEEELKSRRRFVSELDIELNNIQLKMGAAPSTP FT VSTEPYTVDNGASAGLSEEDHAVNRQYQEQLYQQQDVMLDGVYDTIGNIRGQAALMGEE FT LGQQADLLDTLDNSIETTNSKLRRGMKRLKDFTIASADSKSGCCITVLIIILIALLVLV FT IVL" FT misc_feature 10439..10444 FT /note="gtaagt, splice donor sequence" FT misc_feature 10508..10529 FT /note="ctgacatttattcgaaatatag, splice branch and acceptor" FT CDS join(complement(12225..12297),complement(12024..12134), FT complement(11439..11954),complement(11311..11345)) FT /db_xref="GOA:Q9HGN2" FT /db_xref="SPTREMBL:Q9HGN2" FT /label=SPBC36B7.08c FT /note="SPBC36B7.08c, len:245, SIMILARITY:Arabidopsis FT thaliana, AAF27100, f6a14.10 protein., (255 aa), fasta FT scores: opt: 287, E():1.9e-09, (30.2% identity in 242 aa)" FT /gene="SPBC36B7.08c" FT /product="putative chromatin decondensation protein; FT nuclosome assembly factor; SET domain" FT /protein_id="CAC05729.1" FT /translation="MEAAQAFENLANLEQEFGKAEIEILKKQNELFQPLFEQRRDILKT FT INNFWVVVLEAAGDEISQYITPEDSVLLEKLENIYVERFNEKEPRDVRISLTFQPNEYL FT QDDNLTLVKEVRIKEEKAKDDEGLEKKITKYTSQPVDIHWKPGKSLFRKNKKLPPNFFD FT YFQWTGEEEDDDFDGATLTIFLAEDLFPNAVKYFTEAMTEEASDEDESVDLEEDEEEED FT EEDEEGDEEKQEPPSKKSKKSN" FT misc_feature complement(11346..11363) FT /note="ctaacacaaaaatcgcag, splice branch and acceptor" FT intron complement(11346..11438) FT /note="confirmed intron" FT misc_feature complement(11433..11438) FT /note="gtacga, splice donor sequence" FT misc_feature complement(11955..11972) FT /note="ctaattaagttatttcag, splice branch and acceptor" FT intron complement(11955..12023) FT /note="confirmed intron" FT misc_feature complement(12018..12023) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(12135..12154) FT /note="ctaatgtatcttggtgctag, splice branch and acceptor" FT misc_feature complement(12219..12224) FT /note="gtatgt, splice donor sequence" FT CDS join(13411..13527,13577..16169) FT /db_xref="GOA:Q9HGN1" FT /db_xref="SPTREMBL:Q9HGN1" FT /label=SPBC36B7.09 FT /note="SPBC36B7.09, SIMILARITY:Drosophila melanogaster, FT O61651, eif-2alpha kinase., (1589 aa), fasta scores: opt: FT 807, E():0, (29.1% identity in 955 aa)" FT /partial FT /gene="SPBC36B7.09" FT /product="eif-2alpha kinase" FT /protein_id="CAC05730.1" FT /translation="MDAAKRLELCKEIQENEIEALKAIFMDDFEELKVRNAWNVTNGHV FT YCIHLCSRSANSKSIAKLDLCIELGRSYPYVKPVIKLQNGENVLNSQIRFLLDKLDTKA FT KDLLGEEMIFELASIVQDYLNDWQSDLSSQFASLEEERAVQLKHDRERAEVDLQLRLKR FT EKDALFEEEQTLQNKIQDELQRRSYETPQSSSKKKTNSKETTSLETLPTSIYFDCSISV FT RDCHDSLVTFNRVLPLYTISHSNLSTLTLVKPESKEISLQDCVFLLRTVRISTPYWSTE FT DGKREIQELEYELESLKVIRHDLLASIYEYQLERETRGYGWRLYVLQEYSPKFTLFSLL FT QTVLTLDVETVRAFSNNILEGLAELHRLGISHKSLHLDNVVLFHSGHRTFAKLMDFGFT FT RTLRDMNASHPFNINSQSITNILPEGLYPPEVSESSFAAASRKTDIWCFGLLVLQMLCG FT AHVLNKFSSLKLIMTHVIPLLPGSYQDLVRRCLMRDSRKRPSAIDLLSSHVIRLGTAVL FT PPVEQGTFSKSARPSYGGQQDGIIDLLYRKSVSRYETDFEELEFLGRGGFGEVVKVKNR FT IDGRFYAVKKLVLLSDDKENSRILREVMTLSRLHHEHVVRYYTAWVETEANDTVTEIIS FT SDSESLSQSLNMAVDFRQSSSLPADKLSSLDIHFEDDYNSSADEEDPEASDISFQYSNT FT SDKEGSSDKDSSIEEASSVKTQENGLNATLYIQMEYCEKLSLQDIIRDKIPVDEMWRLF FT RQILEALAYIHSRGMMHRDLKPGNIFLDENRNVKLGDFGLATENENYQDNNDKWKNRQS FT ADEDLTTGVGTALYVAPELLSRRNGVRYDAKVDMYSLGIILFEMCMTFSTSMERIRIID FT TIRSPSISFPSTFPFSRASHEFKVIHCLLQHD" FT misc_feature 13528..13533 FT /note="gtatga, splice donor sequence" FT misc_feature 13559..13576 FT /note="ctaacaatggtatcttag, splice branch and acceptor" FT misc_feature 14309..14980 FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 47.56" FT misc_feature 15125..15313 FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 61.80" FT misc_feature 15629..16030 FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 145.05" XX SQ Sequence 16169 BP; 4936 A; 2841 C; 2951 G; 5441 T; 0 other; atggaagttt ctctgatctt ggcatttttc gtttcataat gctgatcttg caaatctttt 60 tgcctggaat tgtctgtatc tatctttgcg aaattattga aaaaggtcat ggcttaggat 120 cgggtcccgt tttactttta ggctcgcaca tattaggcaa catcatgtgg gatgttttga 180 gtttacaccg ttatcctgta aacgagtctg gtgattccca atatcagggt gctcttgttg 240 gatttgcttt taacttgttt tccttcaaaa ataagttttc ctcacttcgt tccattcttt 300 ttcgtagtga aggtttaagc tttgttcaat tcctggtttg catagctgtt tttgcaactt 360 tcatgtacac actaaacatt cgcattgatg tacctatccg ttcttcgcga gttcggggtg 420 ttagacagaa ttttcctttg aaattacttt acacatccgt tattccactc atttactttt 480 attccatcct ttctcatctc ttagtgtttg cctacgcatt gtacagttta tgtccaaatt 540 cattgataac ccgcttattg gtacaatatt caccaattga tacgtttgct gaacacaaat 600 tacaacttgt tggtggtttg gtctactttt tgtaccctcc tttgggttta tcggaagctc 660 ttcttcatcc tgtacataca gttatataca ctattacttt gatatgtatt accatatatt 720 tctctttgtt gtggatgaat gctacggctg gtggacctcg tgatgttttg ctattcttca 780 aagaaaatca gcttgttata gctggttatc gtgaggccac tatgctgaaa gaattggaaa 840 aaatcattcc cattgctgca aaactttctg ccttttttgt ttcaatcttg tccgtgattg 900 ctggaatatt tgcttctact tttggtgttg gtgtccttat cgcttccgct ttggtatatg 960 cctctttcga gatgattgtt ggcgcaaaca catctcttgg taatggtaac tgaaatccgt 1020 tatactttca aggatagaaa aattcagtaa agaacgtctc atttactgta aaacattgtt 1080 gtttactacg actgtattca ttttttttct tataccgttt ttttttttaa acttatatcg 1140 aatacatcaa tcactatttg aaactgacgt ttttttttat agaactttat ggtgttgcaa 1200 agctctacaa caactattta aagttgttaa aattatggca tcaaataaaa ggattgtaaa 1260 tcaaaattgt gtagcaaaat aaatcaactc gttttcgttt taaggagtca ataaaataag 1320 aggcagtatc cgactaagta cttcatttat tagcttgact tgcactgttt ttcagctttc 1380 tgcaaaattt cgatattact cgtgcgtttt taaaagctca tacaatgatt ttgaaatgta 1440 aaatttaagt tttcgataaa gttagtcata tctaatttta aaattatttt attgctacga 1500 aaagcgattt catgaatgta taattttacc ataaagagaa gtcgaagaca aaatcgtatc 1560 ttattcagat aatatatgac tctatagaag aatatccagt ttgatataac tctattatag 1620 tttattgaca aattatataa aaaatgttta tttagacagt atttcgttga gtagaaagcc 1680 tcggcaaatt ttgtctgacc tacgtatatt ctacatattg atttggtcaa ttttgatcat 1740 actggagaaa acgcatgttg tttcgaaagt tttggaacaa acagatcaat caacccacca 1800 ccaaggcgca gtgaattgaa atcaacttat aaggtgattt tcaattttaa agtcgttcgc 1860 taaacaaaaa atctcataaa taggtgtgat ccgtccagct atgcaaggtt taaagttgtc 1920 taatttcgta tttctctaaa ttttgctata tttggatgta caaactgttt tgatttcttt 1980 tctcatatca accatttttt tattttagga gctttaattc attcggttca ttgcaaataa 2040 ccgtcataaa aaggatatat atacataggt gtaaggtttg aaattattcg atcaggcctt 2100 tgctgttgac taaaacgtta cctcaatcta ttttttttaa gctttccttt ttttattttt 2160 atttttaaaa cgttatgtct agttcatata gcagtcgtgc tgaaaaatat gatcttattt 2220 cttgcaaaga cctaacctgg gatttgtcga gatcgcattt aacaactcaa tataaatcgt 2280 tttacatgtg ctttgatgat ggttcatttg gctatcttca aattgcttac ggaaatcttg 2340 gtttactggt aaaagttaca ccattggggt ggacttatta tccagcaaag acggaaaaaa 2400 atgtagagcc aattgtaaac agctctactt atttatacag gagcatgaag atctccaaag 2460 atcgatatag tgttgatgtt caaaagcatt ttatgcattt taattcttct acccgagaat 2520 ggcatatagt tatcgaaggt gtttacgatc taaaaatcaa aaccgaggac agtggtttct 2580 cccttgttga ttttggccaa aatgattctt cgagcaggat ggaacataaa atatttccca 2640 ttaacactgt agaaggcact gttactgcgc atggtaggga gaaaaagcta actggtgtag 2700 ctgtttatgt tggtgcattg ttttttcgag agaaatttac caaccttggg catacttggc 2760 aaaatagtat tttgtttgat aaatctaaaa aaagcatact gacctgcctt cactattttc 2820 ctcgtgaccc ttcacaacct tttcggtctc aagcgagtct aactttggac gggaagttga 2880 tcgctgtttt gcttgacaat gaatattcta ctaagggagc ggctcaagtt gatgggattc 2940 gttatttatt acccgagcag attcatcgga ttttgtctgg acaaactttt gatgggaaac 3000 cagttcgtgt agaactttct gctgaattag aagagttgga tagtataact gatgttctaa 3060 aaattttccc caagtgggct aaagatatcg tttcaatctg ggctggtctc cctttcgtct 3120 ttgtctggtt gcagcatata acagccgagg tttttatcga caatgagcat gtagccactt 3180 tatctggatg ctattatctt gaacaaactt gcgttcacct tcctcagact tgttaaaaaa 3240 cggtgtttta tctttttttg ttcctccaag gattgttggc actatgttat tttatgaagg 3300 aaattttatt ctctataaag ttctttgtaa ctgtctatac ctgtgcgtca atatttagga 3360 tgcccaaata tatatgttta tgttttagtc ggatacagcc aaaaaaagac ctttaaaagc 3420 tggtgagatt ggtaaaatta tcattgacac aaagcgaatt taagttttta aatttttttt 3480 aaaaaaacca gatatcgcgt ctcttaagtt acacctcaac tgtttttata tagtgtaatt 3540 atttattgtt tatagacttt tcttcttgtg tactttagtt tataaagggt gttctgattt 3600 gtgttgataa tacatatcat ttaacagagc ggtttgttaa agatttctta aaaattgaac 3660 atcctgataa ttgaattcat gtaaaaataa aaaatctcaa atatattttg gtaaaacccg 3720 taatatttca ttttagcagc ctagtatatt gcttaaggag actctacaaa ttacgttata 3780 tgatctttgt ttgtcctagt gaaccctgcc tttaccaaac aacttgcaat gtcgtctgag 3840 tacaagtacg acgagcaggg tatttttttc cctgtctttt tgctggtggg gacttcctgc 3900 tgtgtccttc ctttgaccta ttctaccatt cttggtccaa gtggtaagtc agaagaaatt 3960 gctagtgtga tagctaatat ggtgaatttt tagcgtcaaa agaaaaaaag aatgtaagag 4020 atccgttcca aaagtatcgt cctaaggatt taaaagtaca aaggaaatca atatttcgac 4080 ttcggtatga catgtctggt atcattacga tgagtaacta atcagttaat ttttattatt 4140 atagatatat tttcctcatt ctaggttggt tggcaattgg tttcctctct tacaagatag 4200 cgaactcaag attaaaactg aacatttggg atccgtatga aattctgggc attgctaaag 4260 gcacttcagt ggatgatgtt cgtcgtcatt ataaaagact ctcaattaaa tttcaccctg 4320 acaaggtcag gaatatggta aatactacta gagaagaggt tgagaagcat tatattgaaa 4380 tcaccaatgc ctacagagct ttaactgatg ataaaactcg cgaaaattat gctttatacg 4440 gtactcctga tgttcctcaa cacatttcag ttggtatagc ccttccaaag tggatttccg 4500 agtcagaaaa cagcatatat atccttggtt tttatggact tgtttttggt atcgttttac 4560 cgtatgcagt aggcaaatgg tggtatggta gtcgtacata taccagagac catgtccacg 4620 tcgatacagt ggatgagtgg tttccaaaaa tggaaactag cctaacactt gatgaacttt 4680 tgtctctctt tgcttccagc aaggaattaa cttcgttggt gccaaatgaa aagaatccca 4740 aggaatacat tctaaagtta ttgtttgacc atttgaatcg aaaaaagaca aacaatttca 4800 atacacatca aatactttca caatctgatg tcgtacttaa tgccttgctc tctgttgcta 4860 ccgcctttgg ttttgcaaat cctgttgata acgtgttgaa actatggcaa catattgtgc 4920 aagcaattcc tttagatgca ccatttcctt tgttgcaact tcctcatctt ttaatggaag 4980 acgtcaagaa tttatcaatt agaaacattt cttcgattcc tcagtttctt tctttaagtg 5040 aggaacagac gaaggattat cttcccaatt attccaaaaa ccagcttaaa gaaatgcgcg 5100 agatagcaaa tggcattcct cgaatttccg tggtcgcagc aaaggttttg gttgacgatg 5160 atgagtatat cactgtggga gcaattgcaa atttgatttt ggatttgaaa tgctcttatg 5220 gaaccgaagt tgtcccagaa gtcagcacgg acggaacaga gacggccaca aaaaaggatg 5280 aggaagatgc cgaaaaatat caccaatcga aagatgtggt tttgggagat gttgaaactc 5340 ttccatacgc ctgggctcct tactttaccc agcatcataa aactgcatgg tggatctatg 5400 tagtggatcc tcgccaaaat agggttattg taccgccctt tagtatcaca gatattccaa 5460 aaactttgcg tacatttaga atacctttcc aggttcctcc agtcgcaggc acattcagtt 5520 ttcaacttca tatcatgtcc aattcctatg ttggtgaaga tgttattagt aatctaacta 5580 tgattgttaa agatacttcc gtattgcaag agcaattgca agaagaagct gtttcggatt 5640 tagaagacaa tagtgatatc gatgaatcag caaacgcggg taaggatttc agcgatgatg 5700 agaatattgg ttctgatgaa gaagacgaat cggaatatga ccctatggat actgacactt 5760 ctgatggaga ataataccat gcgtcatgac taatccattt ttttctgaat gtgtcataaa 5820 atttaaaata cgtatttctt ccattgtttc atttcttgtt agatgtaaca gacaataaac 5880 cttcatcgtt caaattcaat tctgctaagg ttttttggtc tttgtaatgt ggacgatgtc 5940 tgagtgttaa ataactttta caaattaatt tcttagcata aagggacctt ttagacgccg 6000 gacgcattgc tttgacatta gctgaataac agttgaaata atataatttg cattttttat 6060 tcgttcttat gaacctgttt agtaataatc cttgcattcc aaatatttac atttttgggt 6120 attttataga gtaagaaatt taggttcagt gtgttattaa gattattgta acttttttat 6180 cgagtattct ttcgctacaa atctacaaaa tcctgcctta ataataaatg ttttatcttg 6240 cttaaagtat gcagcctaac ctcttttgct atgccacaga ttccccatac gtcaaatttt 6300 tttggcccag cgcataccac caacggtaaa taaaactcgg aaatatttag gaaaataaat 6360 tctttgaatg tacaactcag agaaaagctg tttttcagaa taatggcttc aaaaaagctt 6420 catggaaggg atttttacaa taaaattggg cgtccaaagc gcattcttgc tccgatggta 6480 gaccaaagtg aactaccttg gcgcatattg gcgagaagat ctggtgctga tttgtgttat 6540 agcccaatgt ttcattctcg attgtttggt gaaagcgaag attatcgaaa caaagtattt 6600 tcaaccagga ctatcccaga agaacgaccc ttaattattc aattttgcgg caatgatcca 6660 gaaataatgc ttaaagcagc caaaattgca gctccttatt gtgatgctgt agacgttaat 6720 ctgggttgtc ctcagggaat tgcaaaaaaa ggtaaatatg gttcattctt acaagagaac 6780 tggaatttga ttgaatctat cattacaaag ttacatacgg agctttctat tcctgttaca 6840 gcaaagattc gtatatttcc tgatccccaa aaaactttag attatgcaaa gatgattctt 6900 aaggccggtg cttccattct ggcggtgcat ggtcgtcttc gagaacagaa gggacatttt 6960 actggtatag ctgattggga gcaaattcaa atgcttcgaa aaaatcttcc ttccgaaact 7020 gtcttatttg ctaatggaaa tattcttcat gcacaggaca tagatcgttg tatcaaatat 7080 actggtgtcg acggtgttct ttccgctgaa ggaagccttt ataatccccg tatttttcta 7140 cctccctcat cacctctcat gactttatac ccacgaatcg atgatatgtg cgaagagtat 7200 cttaacataa tcagagaatt caagttggaa tcagattata gtagtttgtc tgctataaag 7260 ggccatcttt ttaaattaat gagacctctg ttgagtattc acactgatat tcgaagtaaa 7320 ttagcacaag gttgtacccc tcgtgatttt gagacgtttc ctcctgtggt tgctatgctt 7380 cggaaaagat tactagaatg cgaagagaag ggagaaatta atgaagataa agatgttaag 7440 gaatctgtta aagattctat gggttaccct gttattcctt ggtggcgtgt ccagccttac 7500 attcggcctc tggaggttcc tctagtaacc aagcgaaagc cagttgaagt aaccgctgat 7560 gagggacctg taaagaaaaa ggtcaatgct tctgtggcat agtccataat tctttttttt 7620 tgttttgttc tggcattact atactaatat aggcaaatta ttttggtggt acataatcat 7680 tgagttcatt taaaataata tttttactga gattaaaagt tatattatat ttgtaaggat 7740 ataaaatatg cgaaacgatt tgtaaaggca ttaaagtatt agtatatgta taaaaattcg 7800 acatcccaat gtatgattac aatgcaagaa aaacaaattc gaaaagaaag ggcataactg 7860 aaataaataa ccaagtaatg atacaatgtt agatgcctat acctagaaat aataggagag 7920 agaagacagc aatttaatca tgcaaaagca accgaagccg aaattagaaa cctagaagta 7980 atttggaacg atattaagat aaatgcagtg tagcggcatg aacaggacaa ccagcagccc 8040 cagtactcag ccaatcacga taacatttta aatgaaaaat gcatagacaa tattctatcc 8100 ttgcaactct atcaccagca gcaaattctt caaagcatat aatacattct tgccccaggc 8160 aaggagaagt ctctgatagc tgcatactta tgtatcttct agcgtgtttc aaaaattcag 8220 tcatgtttct tggagatgaa ctattattac ttagacaatt cgcaacatga gattcccgtt 8280 cagaaagggt tttaaaactt gagagaggag catagcaaac tggacattca acaaaatcat 8340 caggctcttc aatcctctgc aaattgtttc cacaggaaga ctgtataact tgattgttag 8400 atcgagtagt ggtatcatcg ttcaaacaag tagtgatagg atagggtaaa tccagttctt 8460 ttagttccga caattgctga cgacaattga cacaaactcg tacattgatc catgaagagt 8520 cttgtgaatc attatcatca tcattatcat tagcaagaaa atcaggatct ctaataaaca 8580 tgtcttgtgg gataagatcc tcagttggat ctactgaaac actgctcaca ggaagctttg 8640 caaaactatt gcagcaattg taacaaaaaa gtttaccaca ccatctacaa tgatgtcttc 8700 tccgaaacca ggtaaaagga ccaccacaat tattgcactg cgcactttca tcgtccagtt 8760 gccaaattgc cgccggcgtt tcaatgagta agcgtctcaa agcctctgga taagtcgtct 8820 catcctcgtc catactcaat ttgataaaaa ttcattaaac tatgttcgag agcaaaaagt 8880 tgagtacccg ctagaatggt gttgaaagtt gcctcaggag ataaggtcaa aacaaaaggg 8940 taacttatac tgatcaaagg aatatgatta ccggtggaag tggaaaactc actgcatatc 9000 gcaaaataaa ttttttaaac ttcgttaacg taagagaata agataataag tcatttagaa 9060 tatcaattgt agtaaatatc aaaaagctat ccaagaagaa taactttatc agaaattacg 9120 catttaaaca tcttttcttt atgtatgttt catagagctc agaagcaaat ttagcgccgt 9180 ttcatttaga ccttaatata acgatgtcag cgtgtttgat gaaagttgac tttcttcgtt 9240 attatcaagc ttggtttgag gcagtatgac attagatctc tgaaatttaa aaattgtcga 9300 gaatagcttt atgagttgag gtttcaatca tctttagttt ctttaaattt attgaggtta 9360 gtaatatgac aatataattt gtttttatgt ttacctccat tgcacatttg agcttatttt 9420 gggaattacg acgatgctga actaaattct tctcaaaatt ctgggaaagt gcctgaaatt 9480 cttccaatgc atattcttgg gaagaaatag aagagatctt actattgacg gactccaatt 9540 ctgcgtttat actattatat tccaccaaca ggtttgacag gtttttcgaa gcatccagct 9600 gtaatttatc taaagcacga agcttctcct ctgtcgttct taggctttct aaaatcacgt 9660 ctctgaattg ttgggtcaat tagctgaaga aacttatgga cgtacttgac acgtgcattc 9720 aaaaaatatg aatgcaatgc actatcatga tgctcactga atgctaatac tgcttcagat 9780 accatgttag catggcgatt gctcagctct gtttgcaaag acgttataat ttcagacatg 9840 ttgtggttct tgataacatg ttaatagtgt ctacgcgtct tttgtttaca tcaagctagc 9900 gatatatagc gtactgtttt agctcacaag actttctttg tgttcagttt ctcttaaaac 9960 atttagtctt aggtttttag caatagaatt aattttttag cacaaaggct agaatgaaag 10020 attgcttttg gccattgaaa acgttttatt tacctagatg tttacattta atctaacata 10080 caacggtgga atacggttga atcaaaacaa aatagtgaca aggggaggtt gctgtttttt 10140 caacaagatt gagatattgc tttcttattt attctccatt acttgttttg taaggactca 10200 atgtttacgg tgatctttga gtgagcagtt aggtagcgtt atctatcgta aagtcgttac 10260 ttgactgaat cagcacagta ctttagtatt tgcagacagc atacctgata atacgaattt 10320 gcttcgttta ttttatatta tcttattttt ctttaaatta taaaagcatt tccaagtaga 10380 aaattggctc atatctctac atcatcaaca tggaggaccc cttttatgaa gtcaaagcgt 10440 aagttgtgca atgatgaacc agaaatgtct taatagattc cttttgttgc tttaagattt 10500 tatttaactg acatttattc gaaatatagg gatgcatcaa atcaaatgga gcaagtgcga 10560 aagctttaca attcttttat ggccgcacga aactctggag tcctttcacc aaatactgag 10620 cttacttacg ccattgatga gttaagcgaa acattgaagg acttaaaagc tgctgtggaa 10680 atagcgatga aaaattctga gcattttggg ttagatgaag aagaactaaa gtcgagaagg 10740 agatttgtct cggaactgga tattgagtta aacaatatac agttaaagat gggtgctgcc 10800 ccaagcacac cagtttctac agaaccttat actgttgaca atggtgcctc agctggtttg 10860 tctgaagaag atcatgctgt caatcgacaa tatcaagagc agctttatca gcaacaagac 10920 gtaatgctgg acggggttta cgacacgatt ggaaacattc gtgggcaggc agctttgatg 10980 ggcgaagaat tgggtcaaca ggctgacttg ttagatactc ttgacaattc cattgagacc 11040 actaatagta agcttcgcag gggtatgaag cggttgaaag attttacgat tgcaagcgca 11100 gactctaaat ctggatgttg tattaccgta ctcattatca tcttaattgc attgttggta 11160 ttggttattg tgctgtaact aaatctttat attgtattat ttaattcttt gaaccatctt 11220 cccaataaac gaataaccaa tcattccata ggtaacctcg agctttggga agacaaaaaa 11280 aatttcatgt ctgtgttctc gttccttaaa ttaattagac ttcttggact ttttggaagg 11340 tggttctgcg atttttgtgt tagttttaca atacattgac atctatcata tcatctattc 11400 atgctttcgc cttatttttt taagacctgt tttcgtacct tgcttttctt cgtccccttc 11460 ttcgtcttct tcgtcttctt cttcctcatc ttcctccaaa tctactgact catcctcatc 11520 actcgcttct tcggtcatgg cttctgtaaa atattttaca gcatttggaa aaagatcctc 11580 tgccagaaaa attgtgagtg ttgctccatc aaaatcatcg tcctcttcct ctccggtcca 11640 ttgaaaatag tcaaaaaaat taggaggtaa ttttttattt tttctaaata aactctttcc 11700 tggtttccaa tgtatatcaa caggttgcga agtatacttg gttatctttt tttccaaacc 11760 ctcatcatcc tttgcttttt cctctttaat tctcacttct tttaccaagg taaggttgtc 11820 gtcctgaaga tattcgtttg gttgaaaggt aagcgaaatt cggacatccc ttggttcctt 11880 ttcattaaac ctttcaacat aaatattttc gagtttttca agtaacacac tatcttcagg 11940 tgttatgtac tggcctgaaa taacttaatt agaatttcaa caatgtttaa acaaaaacaa 12000 taatagtata caattttaca tactgatttc atccccagca gcttccagaa ctactaccca 12060 aaagttgtta atggtcttca aaatatcacg acgctgctca aaaagcggtt gaaaaagctc 12120 attttgtttc ttcactagca ccaagataca ttagcaattg ttaacacatt ttttataaaa 12180 aacgtaattt tatgctacaa aaatgcaaaa tcgattaaac atacatattt cgatttctgc 12240 ttttccaaac tcttgctcta ggtttgcgag attttcaaaa gcttgagctg cctccatttt 12300 gtaggttaat ttcgtgcaag ggagaaaaat ctaatagtaa ttcgaaaatt ctcacttcgt 12360 caacgatgat gaaggcacta cccttaccac gtttttacgt acgatgaaca atgctagcaa 12420 agtatatcag aggctttact ttccaatata atgactgccg tagcttctgt agcttgcaat 12480 aatttaacct tgactttttc aacagtatac aaaccatcca tattactagt aatcgagcag 12540 gattctagtt tttaactcac accattttca taggttgttt ttataagtgt aataacttat 12600 tagtctgaac aaagagtaaa attagaaaat aaactgtagt ttgcgcggtt tcgagctttc 12660 aggctagcgt gcttgcattt tccaaacttc tcctgaccat gatgaagaaa ggagaggagg 12720 aaaaaaggta taacatttcg aatgcaacta caaatttgta ttatattgca ttatatgaaa 12780 cgctgttata gtttactggt gtatctattt tatcagttat gaagtatgtc tttgtagttt 12840 aatcaaaaat ccatgattat ggtaaaacaa tgaagaaaag ttaaccaaac gcgattaaaa 12900 gttaaaactt tttgaccgct tcacttcaaa caatgaagcg ctttgcatac aaatgcagat 12960 aatcattata tccaactgtt cgttatcgat tgaagatctg aagtctgatt catcttatcc 13020 aactcaaaat tacatcttaa ctaaagaatg tatgatccgt gccgattatc gcatttattc 13080 ggctcaaatc aatctatact cattacaata ccagtatact gcgttcttcc atctttagta 13140 taccacgagc gagaaacttc ttcctccagc aaattcgcgg caaacaagca atttatctaa 13200 aaatccaatt cagaggaaag aaaagaaata gaattgataa taattatatt ttcaagtcct 13260 atattgccgt cctgatataa actgatttgc tttctttcct tccagtttac acaggacgaa 13320 ttttcaacca acagtttaat tgtgaataaa tatatttgct tcctattaaa ctagtaaatt 13380 attggcaaca atagttggta aaagctgttc atggatgcgg cgaaacgtct ggaactatgc 13440 aaggaaattc aagaaaatga aattgaggcc ctcaaagcca tttttatgga tgattttgag 13500 gagttgaaag ttaggaatgc ttggaatgta tgattaatgt ttgttcacta cccatttact 13560 aacaatggta tcttaggtta caaacggcca tgtttattgt atacatctat gttcaagaag 13620 tgcaaactct aaaagtattg cgaaacttga tctttgtatt gagcttggaa gaagctatcc 13680 ttatgtgaaa ccagtgatca agttacaaaa tggagaaaat gtcctaaact ctcagattcg 13740 atttttgctt gataagttag atacaaaggc aaaagacttg ctaggagagg aaatgatttt 13800 tgaacttgcc agtatagtgc aggactatct taatgattgg cagtctgacc tctcttctca 13860 atttgctagc ttggaagagg aacgtgcggt acaactaaag catgatcgag agagagcgga 13920 agtggattta caacttaggt taaaacggga gaaagatgcc ttatttgagg aagaacaaac 13980 gcttcagaac aaaattcaag atgaattaca acgccgctca tacgaaacac cgcaatcttc 14040 ttctaagaaa aagacgaata gtaaggaaac aacttctctc gaaaccctcc caacttctat 14100 ttattttgat tgttctatat ccgttagaga ctgtcacgat tctttggtta catttaatag 14160 agtgcttcct ttgtatacaa tctcgcattc caacctttct actttgactt tggtcaagcc 14220 tgaatctaaa gagatatctt tacaggattg tgtttttcta cttagaaccg tccgaattag 14280 tacgccttac tggagtacag aagacggaaa acgtgaaata caggaattag aatatgaact 14340 tgaaagtctg aaagttattc ggcatgattt actagcttca atttatgagt atcaactgga 14400 aagagagaca cgaggttatg gttggaggtt gtacgttttg caagaatatt ctccgaagtt 14460 cactctgttt agtcttttac aaaccgtcct tactttagac gttgaaaccg ttagagcttt 14520 ttcaaacaat attttagaag gtttagctga gctgcatcgt cttggaattt cacacaaaag 14580 tttgcacttg gacaatgtgg tgctttttca ttcaggccat agaacttttg caaagctaat 14640 ggattttggg tttacacgca ctttacgaga catgaacgca tctcatcctt ttaacattaa 14700 ttctcaatcc attaccaata ttctaccgga agggctgtat cccccagagg tttctgagtc 14760 atcatttgca gctgcatcga gaaaaactga catctggtgt tttggactat tagtattgca 14820 gatgctttgt ggagctcacg ttttgaataa attctcttca ctaaaactga ttatgactca 14880 tgtaattcct cttcttccgg ggtcttatca agatttggtt cgtcgctgtt taatgcgcga 14940 ttcaaggaaa cgccctagcg ccattgactt gttaagtagt catgtcattc gtttgggtac 15000 agcagttctt cctccagttg agcagggtac cttttcaaaa tctgcacgcc cttcttatgg 15060 tggtcaacag gatggaatta ttgatttgtt gtacaggaaa agcgtttcac gttatgaaac 15120 agattttgaa gaacttgaat ttttaggacg tggcggtttt ggtgaagtag ttaaagtcaa 15180 aaatcgaatt gatggaaggt tttatgcggt taaaaagctt gtccttttga gcgatgataa 15240 agagaatagc agaattttac gtgaagttat gactttgtct cgtttacatc atgaacatgt 15300 agtgcgttat tatactgctt gggttgaaac tgaagctaat gatactgtaa ctgagataat 15360 tagctccgat agtgagtcgt tgagtcaatc tttaaatatg gcagtggact ttcggcaatc 15420 ttcaagtctt ccagccgata agctttctag cctcgatatt cactttgaag atgattataa 15480 ttcatctgca gacgaggaag accctgaagc ttctgacatc tcctttcagt attctaatac 15540 ttcagataaa gaaggttctt ccgataaaga cagcagcatt gaagaagctt cttcagttaa 15600 aacacaagaa aatggtttga atgctacgct ctacatacaa atggaatatt gtgaaaagtt 15660 gtcattgcaa gatatcatac gagataaaat tcctgtggat gaaatgtggc ggttgtttag 15720 acaaatttta gaagctttgg catatattca cagtcgggga atgatgcaca gagatcttaa 15780 gcctggaaat atttttttag acgaaaatag aaatgtaaag cttggtgatt ttggcttggc 15840 tacagaaaat gaaaattacc aagataacaa tgacaaatgg aaaaatcgcc aatcagcaga 15900 tgaagatctc acaacaggtg tcggtacagc tttgtacgta gctcctgaac ttctcagtag 15960 gcgtaatggg gtacgctacg atgcaaaggt tgatatgtat agcttgggta ttatactctt 16020 cgaaatgtgc atgacttttt ctacttctat ggaaagaatt cggataattg atactattag 16080 atctccatct atatcgttcc cttccacttt tcctttttcc cgtgctagcc atgagttcaa 16140 agtcattcat tgtcttctac aacacgatc 16169 //