ID SPBC32C12 standard; DNA; FUN; 8844 BP. XX AC AL022172; XX SV AL022172.1 XX DT 18-MAR-1998 (Rel. 55, Created) DT 27-NOV-1999 (Rel. 61, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c32C12. XX KW high mobility group; HMG-box; protein kinase; KW serine/threonine protein kinase; ste11; transcription factor. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-8844 RA Wood V., Rajandream M.A., Barrell B.G., Badcock K., Churcher C.M.; RT ; RL Submitted (17-MAR-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O59697; O59697. DR GOA; P36631; P36631. DR SPTREMBL; O59697; O59697. DR SWISS-PROT; P36631; ST11_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 80% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c32C12 is overlapped by cosmid c3B9. XX FH Key Location/Qualifiers FH FT source 1..8844 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c32C12" FT /map="IIR" FT misc_feature 1..102 FT /note="nominal overlap with cosmid SPBC604, EM:AL132720 S. FT pombe chromosome 2" FT misc_feature 1..4008 FT /note="temp" FT misc_feature 1061..1426 FT /note="previosly annotated as protein SPBC32C12.01, FT unknown, len:121aa but has poor correlation scores, no FT similarity and lies within low complexity gene-free region FT with (A-T)/(A+T) bias, many small A/T repeats" FT CDS 4009..5415 FT /db_xref="GOA:P36631" FT /db_xref="SWISS-PROT:P36631" FT /label=ste11 FT /note="SPBC32C12.02, len:468aa" FT /gene="ste11" FT /gene="SPBC32C12.02" FT /product="transcription factor; HMG box" FT /protein_id="CAA18162.1" FT /translation="MSASLTAEQKDQKSSVKRPLNSFMLYRRDRQAEIPTSNHQSISRI FT IGQLWRNESAQVKKYYSDLSALERQKHMLENPEYKYTPKKRSTVRRRHKKVSPSSGSFV FT ASDYVVLQQIAQSSKTLKQTEPEKPVNEEETLAALLAPALSYPKSGKSNLIETSELSCL FT SSSPMIRSHTIPSLSFTDQVSTTISTLDKSEQAPSSLGIYYRSPSSGSPIGRTKSVCLA FT NKARIVPKRSMSSDGCVDKSYQMSKTPSLEANLPQNSSNCSARRVPKFDSKGTVSEQSN FT SDSPELSADKVLSHCSPIDARPSTPSCPNASISPKTPNTGDHYGFDGAEYLGTPLSVGS FT TTAYLYGQETELLSTPYCHTSYPAMSRLNSSSGYTCVSSSSVTNSGHTENNTWRSDEQS FT KGFVDINSFSQSLFSNGNYEFAAHSQELDDLFSQITDFTSTDPIASSLKDANSLGPSLL FT EPWLPNSNLF" FT misc_feature 4054..4248 FT /note="Match to PF00505 HMG_box, HMG (high mobility group) FT box Score 66.22" FT CDS complement(6438..7709) FT /db_xref="GOA:O59697" FT /db_xref="SPTREMBL:O59697" FT /label=SPBC32C12.03c FT /note="SPBC32C12.03c, protein kinase, len:423aa, similar eg FT . to S. pombe, KIN1_SCHPO, P22987, protein kinase kin1, (89 FT 1aa), fasta scores, opt:874, E():0, (47.8% identity in 314a FT a overlap)" FT /gene="SPBC32C12.03c" FT /product="protein kinase kin1-like" FT /protein_id="CAA18163.1" FT /translation="MKPNTTNLRNECWDTFSIPKRSQNIKINQSTKHQRSISDFVGTAG FT PGRQVGNWIIKKTIGAGSMGKVKLVVNILTGEKAALKMIPFTPNNTSQTVRVQREALLG FT RLLRHPNICRVIDCIRTPACTYILFEYVPGGQLLEYILARGKLDEDLARSFAMQLINAL FT VYLHKNFIVHRDLKIENVLLTQDSRQVKLIDFGLSNFYSKDDLLRTYCGSLYFAAPELL FT DAKPYIGPEVDVWSLGVVIYVMVCGRVPFDDVSVPMLHSKIKSGKLEFPSYISEDCCSL FT IAAMLNVNPRKRCSLEQAAKFPWLKKNSFCLYLPIPLTSIPSTPSIRSHVFKPPFNLKV FT LQLLHEHGLASIPELKHELYMAYIERKTTSLVCLYLLGVESLAPALRIPTALPPVYSRH FT QRHHSEILGAMDLTEKITAMQCPP" FT misc_feature complement(6795..7553) FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 305.34" FT misc_feature complement(7161..7199) FT /note="PS00108 Serine/Threonine protein kinases active-site FT signature" FT misc_feature complement(7464..7535) FT /note="PS00107 Protein kinases ATP-binding region signature FT " FT misc_feature 8744..8844 FT /note="nominal overlap with cosmid SPBC3B9, EM:AL022070 FT S.pombe chromosome 2" XX SQ Sequence 8844 BP; 2483 A; 1744 C; 1313 G; 3304 T; 0 other; gatcaagagt cacccatata taggatggtc aattttttca ccaaattgtt tttctccatt 60 tttttcattt actgtctatc caacatcgat tagccgcaat gagggattat acaagtcaac 120 aatgataagc acagttggct agcagagtcc agttccaaaa ggatgcaatt ttggtgatat 180 gtaagaaaac attccacaat acaatttgtg aagtttttag ctataaattt atcggtttag 240 gttattttca aagtatagtc gttttcaaaa aattatattc gttttcaaat taaattcgtt 300 atcgttcagt agtaaccttt ttcattcaaa actcgtcttc atctataact aatctacatc 360 ttcatcttca tctttacctc tactttctcc catctctcct tcctatcgtc tccttcctct 420 ctgcatcttg ccggccgcca ttttctgact cggcgggtca tccacctttt aagctcaacc 480 ttgttttgct ggtcgctcgc tcaattctta caaaagccca ggttaatagt gcgattgggt 540 gatatgcaat ggaaattagc gcatatctca agttgtacgc acacatacgc taatctggcg 600 gcaattccta ttttctatta cattgcataa gtcaaagcta ctttgcacaa attcaagtag 660 cattcatttc cttctctaaa ataatgtcta gtctttggac ttttatcgtt ttcaatggtt 720 ttatttcttt tttccattcc atttattttt tttttgaaat tttcaccttt ctcaatttat 780 tcgtcgttct ttttttaata taataagatt atacttttac agcatacatt gtgtctcaca 840 tagtctcttc catcgcgttt cccattttat gatcttgcta aaaaaatgtc attcttatgc 900 aaatcaccct gtactaccac agcacttacc tttccgacat tcttgttctc catcttacat 960 gtctatcact gtagcgatag attatttgtg gtgcatgcca tctccaggga tgatggaagg 1020 gaaacacaag cacccacgcc taattttcgg taaattgtag caaaattcca atggcaatga 1080 ctcgatagaa tcattgtatg tgtgtggttt ctttccttcc gctcgtagta cctgtatttt 1140 tcatcatgac tccaagtctt cgccattctt atttcactcc cgttccgttc accccaaact 1200 acttcctcat cacttgctta ctactcaaac tgccggcact tgttttcaca cattcgctat 1260 gcttttttat tcttttaaca tttgcttaaa ccttacctat aataccctgc ggtttcgcaa 1320 attgccgctc ttcaaatgcc cgcttactct attttgcttt ctccacccct ccttaactct 1380 ccaacttccc cagcgttcta caattcctca ttcattggaa gactagtcgt tggcttcttg 1440 tcattttgcc ttgcgctatc tccctcaacg aaaagtagct ttgcgagact ttttgccaag 1500 tgccacacta atcttttgta caggggggaa tccctgactt tgtttgctgt gttacatcgt 1560 ttgtaggcaa gtaaaagtga caaatgcttt cgcatcatat gttactttaa cccctgtgaa 1620 ttttgtccct tcccctcata cacattttgg ctagacttaa aaaagttgaa tttctttgtt 1680 tgattttcat tgtcagtaac atcgcgtatt acgcctcgct ctatactctt actagttggt 1740 tgccaatgcg tatgtatata tatatatata tacatacata cctggtgact tacatagtga 1800 gaatctattc atttagcata tattaaaagg atatccagcg ttgaattgtt tcgcgtcgta 1860 tattggattt tctttattat caaataaaca aagcattatt agcattctga cttttcaatc 1920 ttaaacctga ttgtgaatgc taaaagaaag tatttcattt gattagtttg cattttattt 1980 ttctttgaga gcttttgcct cgtttttttg ttttctcttt gttttctttt ttttttttac 2040 tcaacttaac ttaacttttc tggtctttgt cttgattgca gcttgtcgtc tttgaattag 2100 cttgcctcct cccattcttt cctgtctatt cgtgttaagt ctattccttt atcttggttt 2160 gctttggctt agctctattc ttttctttgt tttgacttta ctttgactta ttttcactga 2220 ctatttctaa gtttaatttg ttgattaatt tttattcaca tctcttcttt tctttatagt 2280 aaacagactt aaactttttt ttcttcttct attctgtttc ttcattttgt tggtccaatt 2340 ggcttcgctt ctctcttcaa acttttctcg tttttcttgc ctctacgttc ttttgggatc 2400 ctcctctgtt ggaattgtta attgagaagt gttggaataa ttttttcgtt tcttgaaatc 2460 aaaaggattt tatctttttt tttaattttt acgtcgattc ccttactgta cgtcgaatcc 2520 cccccttttt tttatttttt ttctacgtca aaattttttt atatatttct ttgtctctac 2580 caccatagtt atcctttatc aactttctct ctactaatta actaagttgt ttctttgttg 2640 caatttttgc ccaactttaa atttttttta ttcgcatcgt tattctatcg cttggctgct 2700 tatccttgat atctgatcta ttagattttc attcctcatt ttattttaat atcctttttt 2760 taacgtgttt cttttgtgat tccattagta cacaccattc agaattaagt tgcattcacc 2820 atcggtcttt gttggctcat gttataacat tatttctccc ctgtcattgt cgcattattc 2880 acgtattcat tggtattcct cactttattt gtatacccaa agtttcacca tcttacagag 2940 ctcaaccatt cttcagttat cggtgtctct gaaatttctc ccttgttacg cctttttatt 3000 tgactcactt ttccgttatt tagtcttccg tttcgtctgt ttttcctgtt aactattccg 3060 ctctttcttg tttttagttt ttcctccgtt ttatatagag aaaaaaaagc cttagaggct 3120 cgtgattttg ttctcgtttt caaatctcct caacttaaaa ttaggcttat aagaaaatca 3180 cttttaaagc ttcaattttt ttcttgtggc cttctattat atatcacttt cactccttct 3240 tccacttgga cctctactaa ttgtctcttt caagttatcc ttgattcaat cgcctttacg 3300 aaaaatatcc ttcatcattc caactcgatc tagtaacttt tcagtctcaa ctttcatctt 3360 tttttttcta cgcttcttta catttttcaa atcggctttg agtgttgctt ctgttttttt 3420 cttcttgagc tggaaattcc attagtttca tttattattt cattacctta cttttttcgt 3480 ttaggaaaaa cctggcatca aatatacttt tgtcactttt ttcttttttt tagtccagtc 3540 agtctctatt cattaagctt gaacttgatt tctttggcga acaagcttgt ttaatatcta 3600 gattgacgga aattttattt tcatttattt gttgatacat acaagggaat tttactttta 3660 ctatattcat ctgttacttc actgtgaata atttgttcat ttttcattct tgttagtttg 3720 agatttcttt ctatcttaat accatttttc tttttttaaa tagaaaaaaa tccttcacac 3780 cagtttttat tcggtgttta tttctttact gttttctgat ctcatcccct ctccctcggt 3840 ttgtgctgtt cgcttctttt ctacttcgtt ctttctgact gcgaacttca tttatgtttg 3900 ctttgttctt actgaatcat ctttctaatt aattctaaga accgtttcat ttgttttatt 3960 ttctccctta cttttactac aatttttata tttactcttc tctacacaat gtctgcttct 4020 ttaacagccg agcaaaagga ccaaaaaagt tctgttaagc gacctttaaa cagttttatg 4080 ctttatcgtc gagatcgcca agctgaaatt cctacttcta atcaccaaag tatctcacgt 4140 atcattggtc agctgtggag aaatgaaagt gcccaagtca agaagtatta ttctgattta 4200 tccgccctcg aaaggcagaa acatatgctg gaaaatcccg aatacaagta cacccctaaa 4260 aagcggtcta ctgttcgtag acgccataaa aaggtatcac cttcttccgg atcttttgtg 4320 gcctcggatt acgttgtact gcagcaaatt gcacaatctt ctaagactct aaaacagacc 4380 gaacccgaaa agcctgttaa tgaggaggaa actttagcag ctttattagc tcccgctctt 4440 tcatatccaa agtcaggtaa atcgaatctt attgaaacca gtgaattgtc ttgtctttcg 4500 tcttccccaa tgattcgaag tcatacaatt ccttccttat ccttcaccga tcaagtttct 4560 acaactattt ctacattgga taaatccgag caagctccta gttctttggg catctattat 4620 agatcaccca gtagcggtag tccaatcggg cgaacaaaat ctgtgtgttt agctaataaa 4680 gcccgaatag taccgaaaag atcaatgtca tctgatggct gtgttgacaa atcttatcaa 4740 atgtcaaaaa caccgagctt ggaggcgaac ttgcctcaaa attcaagcaa ttgctctgct 4800 cgaagagttc caaaatttga ttctaaagga accgtgagtg agcaaagtaa ctctgatagt 4860 ccagagctgt cagccgataa agtgttgtct cattgtagtc ctattgatgc tcgaccctca 4920 actccctctt gccccaatgc cagtatatca cctaaaaccc cgaataccgg tgatcattat 4980 ggttttgatg gagctgagta cctaggaact cctctcagtg taggcagcac tactgcgtat 5040 ttatacggcc aagagaccga acttttatct acgccttatt gccatacttc ttacccagca 5100 atgagtcgat tgaacagcag ttctggctat acatgcgtct cttcgtctag tgttaccaat 5160 tctggacata ctgaaaataa tacctggaga agcgacgaac aaagtaaagg ctttgttgac 5220 attaatagtt tttctcaaag tttgttctca aatggcaatt atgagttcgc cgctcattct 5280 caagaattgg atgatttatt ttcacaaatt acggatttta catcaactga tcctattgct 5340 tcctcactca aagatgctaa ctcattggga ccttctcttt tagaaccttg gttgcccaat 5400 tctaatttat tttgaaaata tttctatgca aacatggctt tgttttcaac aaacgatcat 5460 ttttaacgcg tcacaaaagc atgcagctga tttgttcatg tcatgcttaa aaatctatat 5520 ttttttaatt ttgcatatct ttgttatgct agatgatgcc ttccataatt tatttcattt 5580 accttttaaa ctctttaatt attccctttt tatgtttatt ataggaatat gtgtatactt 5640 agtttccaaa gagcacattg ggcaacattg aatagttgcc cactaatttt cttttttttt 5700 taattttgaa caatgtctta cgttatgaat tgatttctta catctaattt cctgtcatct 5760 aatttgggcc cgttattaca tatatattgt agttaaaaaa atatatatta tatcgcaacg 5820 taatttctaa tttttctctt ccattacttt cgtaagaatg ttaagtatat atatgttcat 5880 tattttcttt acgtatcaag caatcatcca atgcagtatt gttatcacca tttcactaga 5940 tatactatcg atgaaggttt acttggcacc cacacctact ggggtaatgt aaagataact 6000 caaccaataa attgattaag aataaaggta attaaattac ctgacaaaga aacttataga 6060 attcattcgg tttcaatggc gaaaggtatc tatctataaa tgtttatagc attcggcatt 6120 caaatatttc atgaaaagcg acggatttaa agaagtaatg ctatcaaaaa tcgatcaatt 6180 atattttaaa attataacgc aagtcgtaag aatctttgta tatgttggat tgcaaaactg 6240 tattatttaa gaatgaaaac aaaaagaaaa agccttaact cgtctttacc cactaacaaa 6300 aaaaaaagaa aaaagttaag tttagaaaca atgaaactct ttaattaaag taacgaaaaa 6360 gagccacagg ttttaattaa aatggaactt cttcgaaaac acataataaa tgctaacaaa 6420 aattactcca ggaggaatta aggtggacac tgcatagcag taattttctc tgtcaaatcc 6480 atcgcaccca gaatttctga atgatgccgt tggtgtctac tatatacagg aggtaaagcc 6540 gttggaattc gaagtgctgg agcgagactt tcgacaccca ataaatacag acagactaat 6600 gaagttgttt ttctctcaat gtaagccatg taaagttcat gttttagttc gggaatactt 6660 gctaaaccat gctcatgtaa cagctgtaaa acctttaaat taaaaggcgg tttgaaaaca 6720 tgagatcgga ttgaaggcgt tgagggaata gaggttaagg gaataggcag atacaaacaa 6780 aacgaatttt tttttaacca gggaaatttt gctgcttgtt ctaaagaaca gcgcttccgt 6840 gggtttacat tcagcatagc tgcaataaga gaacaacaat cttctgaaat gtaagaagga 6900 aactccagtt tacccgactt tatttttgaa tggagcatag gaacggaaac gtcgtcaaat 6960 gggactcgtc cacaaaccat aacataaatc actactccca ggctccaaac atcaacctct 7020 gggcctatgt acggctttgc atcaagtaat tctggggccg caaaataaag actcccacag 7080 tatgtacgta ataaatcgtc tttactataa aaattactga ggccaaagtc aattagcttc 7140 acctgacgag aatcctgagt taaaagaaca ttctcaattt ttaagtctct gtgtacaata 7200 aaattcttgt gaagataaac caatgcatta atcaactgca tagcaaaact cctagccaaa 7260 tcttcatcga gtttgccacg agcaaggatg tactccaaaa gttgtcctcc aggaacgtat 7320 tcgaacagta tgtaagtaca tgcaggcgtc cggatacagt ctattacccg gcatatgttg 7380 ggatgtctca ataaacgacc taaaagtgcc tccctttgaa cacgaactgt ctgacttgta 7440 ttattaggag taaagggaat cattttgaga gctgctttct ctcctgttaa aatatttaca 7500 acgagtttaa cctttcccat actgccagca ccaattgttt tcttgataat ccagtttcca 7560 acttgtcgac caggaccagc tgtgccaacg aaatcagata tagacctttg atgttttgta 7620 gactgattta tttttatatt ctgagagcgt ttaggaatag agaaagtatc ccagcactca 7680 ttccttaggt tagttgtgtt cggtttcatc ttctcagctg taaaaggaat ttatgaacaa 7740 aattgtgctt aaataattca attacaaatg aagttaagaa aggaagaagt agtgtcttag 7800 ataaaattat cataaaaaga aaactcactt cacataaaaa gatatattgt tcttagtaac 7860 aacaatacta ttaaagtgtt tttatcactg taacgcttaa cttatgtaaa agcagacatt 7920 taattttcaa gtgagaatcc actcacatgg agtttgctaa aggttgagac actcctctca 7980 aggttcttcc cttggcaaaa ttatttggtt tggtcagtat ggtacacggg acgcgtcgcg 8040 acgttaatta atttgtttcg ttagtgtgcg ttatacaaaa tgtaaacaaa taataaaact 8100 cgtcagaaaa cttaccctta tttaccagta tcttacaaca aatgcattgg atttatgaag 8160 gttacttaaa tttgttcttt ttttacatta aagaaaaaaa attatggttt tgagtattta 8220 aaagcgatgg ttttactttt tttggatttt gcaattttaa gcatcatgtt gatcaattga 8280 tgttttctta taataaatga aatgaatgga taaattttta tcaaaagccc attagtagtt 8340 attttttttt atagctgtta tgataattga tccaataact tagattatac taattgacta 8400 caatgcgttg tgttacttct tttacaataa tggttgaaag tcagatgctt aaaaatgaat 8460 tgttgatttt cagttctaag aaatagtaca ttatacatac tgctcactgt ttgttttttt 8520 ctaccaataa ttaatatcgc ctgaaaagaa ttaaaagtag ctttactgaa atgcttcaaa 8580 atagtagcat gtacttcaat cttaaaatag tgttataaat attttaagag tgagaagaaa 8640 gcttttattc tttattccaa agatccactt ctcaactaat acgtatattc cagcgtagta 8700 catttatatt tcttaaaatt tcatccgcag ctttcctaca catgatccat tcagtttagc 8760 ttgcaaacac gttactaagc aacttaagga tctgcacagt tgtgttaaga ttaattatgt 8820 gaagtttata ccttcgtaaa agtt 8844 //