ID SPBC31E1 standard; DNA; FUN; 16920 BP. XX AC AL049190; XX SV AL049190.1 XX DT 15-MAR-1999 (Rel. 59, Created) DT 17-MAR-1999 (Rel. 59, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c31E1. XX KW ca++-transporting atpase; pgak2; syntaxin (T-SNARE); ubiquitin-like; KW vacuolar. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-16920 RA Oliver K., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (11-MAR-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O59868; O59868. DR GOA; O94651; O94651. DR GOA; O94653; O94653. DR SPTREMBL; O59868; O59868. DR SPTREMBL; O94649; O94649. DR SPTREMBL; O94650; O94650. DR SPTREMBL; O94651; O94651. DR SPTREMBL; O94652; O94652. DR SWISS-PROT; O94653; BMS1_SCHPO. XX CC Notes: XX CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. XX CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. XX CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). XX CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. XX CC The length in codons is given for each CDS. XX CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. XX CC Cosmid c31E1 is overlapped by cosmid c660, EMBL entry SPBC660, CC accession number AL034563, at the 5' end and by cosmid c800, CC EMBL entry SPU41410, accession number U41410, at the 3' end. XX FH Key Location/Qualifiers FH FT source 1..16920 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c31E1" FT /map="IIL" FT misc_feature 1..658 FT /note="nominal overlap with SPBC660 S. pombe chromosome 2" FT CDS complement(1..4786) FT /db_xref="SPTREMBL:O94649" FT /label=SPBC31E1.01c FT /note="SPBC31E1.01c, len:>1595, SIMILARITY:Saccharomyces FT cerevisiae, YNY2_YEAST, hypothetical 178.4 kd protein in FT sla2-zwf1 intergenic region., (1592 aa), fasta scores: opt: FT 640, E():5.2e-32, (22.0% identity in 1589 aa)" FT /partial FT /gene="SPBC31E1.01c" FT /product="hypothetical protein" FT /protein_id="CAB39135.1" FT /translation="MRLPSWLKNSSSWLWTAALSRTVQRRLLAFALRKLIGSILLENVQ FT PEDIDILFSKGVLMLSNLQLNCSFLNAVVSLPMINFTKGTLRRLILRLNVTDIVNLNVE FT LEVNGLSLEIELVPPDESLSSTTYEDAPSQLDILDNVVEYMNKTASQDFEDEVINEGLE FT SEIDGSSHNLLDSILQKCLASTSVLMQDALVYIGTANMSTRLEAKLDFMSFSSVKSNST FT SRLLNINGITVSMVRPISKSNAGSSSPPRSEESFVSMDSSSSIVEGFENDLSPSQVTLN FT ESSIISSNREEESFYSVHDSVTQKKTRTSWLIFQCSGEFRLVFSIESSNLLVIESHVPS FT CVLNINSQVIAFLLYLYGYFLPAPSTPGFSSNKPPLTMLQLDIHISSVQILTHCKLPES FT QDFSMHNDVDELLHTIPSNGAVFEMKINQIQIFNDNNDIDELTMTFSDLDIFMDSVTLV FT SFGKSLQSPCSLIFKKLERIVSLFIHIPGGEISLPLGKALLLQESFVEFTNDISNLQNF FT LSNSDPFKRSIQETFEAPKPFEVEMNQPSFEIIALFDELQLQILNELSTQSLYKLTLRV FT SHLQFTRKSTGSLSSVLIFVKKIGCQSELFNCENSDWTKVSSSTAFHLSNSLFETHDTT FT GTSNFAVSIQQEKHYYPVFQPAPTEFSYPEKHFYFAVDNFNVFISKEVVRLFKTLYETI FT ASSLVTPVTPNKLVTSDYKNVLKIRTRTFSLSLLNDDGSSFALKCIRIKHYMCWAGAQL FT ISLSLRLYNVSAEYLSESLEILPVVSFIRNLRNDEKYLLNADFSYALKRSSGSNDNTIF FT VKITDLGYEHYPTCPWISDLLKTYFPQDPEVPFLAFPDFPFNIKLDLRESIIGLNPRTL FT EAKLLLYLKSLDVEIDALVASNPLNIRIMAAETVVYIIDKLNQSVLEGKTSVLKREILN FT SSLHFPGLSIKDTIEFVVKSLGYVEISQLKNLTLSLVVNAEEGVFSTLITVDNLDAQVQ FT SCADSTELLIKVLSDLGSTEDEEISDCYLALPIEDYAKSLTEVDYNFFENRGIDYKSNP FT TEQSTVLVSSDNDISSQEIKIVDDYYISSNEHSTHASDLASVSSEEFVIDDGSSSIIDI FT SDELQDESSSRDSLKKGIELIEDYYLSQSTSKLESSVEGKNYLLKVKFKDINVNWDLHD FT GYDWEATRATISSAIEKLCDSSSQNDKISPEAKTLLFQSIVIKSFSKVGRLNINHVSEP FT IDSDEFADYLSKSISYHLRLGKSKSKKIGIEIKDLQGSFTVYADSNEPNAVLNDLDIGL FT KDLIIYDHLSTSTWNKFFGRDSRSPSSKNRNQHMNAQIVTVRPLPELNNRELRLEFSVL FT PCKMYIDQDTLDFLIRFLTFQTPSSSETLNTEPDLPFFQSICINATHVTIDFKFKSADK FT VGLRSGKLPDLGSLIVMQGSEVFLRQLQIYGLSGAEEFLNALLNVWLQDIRNNQLSKVL FT NGVVPIRTMFTVGRGIKDIFVSPVRGLQGNHSVGSFRHGIIKFTEKYVNDFLSLNAQGA FT TGTHSLLRQAESYLERGSNASASASRARSYYAEQPETIEQGLRQGYSGLKQGLLGAKST FT LMGLPRETRSHKSLGG" FT CDS complement(5643..8342) FT /db_xref="GOA:O59868" FT /db_xref="SPTREMBL:O59868" FT /label=pgak2 FT /note="SPBC31E1.02c, len:899" FT /gene="pgak2" FT /gene="SPBC31E1.02c" FT /product="ca++-transporting atpase" FT /protein_id="CAB39136.1" FT /translation="MSVQYDAFSVEQTCADLETDMYNGLSSLQEITRRNKVHGDNDLKV FT EDEENMVVQFLKQFVKDPLILLLFASSAISVTLGNIDDAISIALAIVIVVTVGFVQEYR FT SEQSLKALNNLVPHYCNVIRSGKTEHIVASKLVPGDLVILQIGDRVPADLRIVEATELE FT IDESNLTGENSPRKKSSEAISSNISLTERNNIAFMGTLVRHGHGRGIVVATGSDTEFGR FT VFLTMQQTEKPKTPLQNSMDDLGKQLSLISLIGIAVIVLVGFFQGKNWLEMLTIGVSLA FT VAAIPEGLPIIVTVTLALGVLRMSKKRAIIRRLPSVETLGSVNVICSDKTGTLTMNHMT FT VTKIYTCGMLAAFSLPESEHIELSVRRTVGIEKALLAAALCNNSKVHNKADSILDTTCP FT WAGFPVDVALIECSERFGLKDPRETYSRISEVSFSSERKYMSVAVQYNSSKMNFMKGAT FT EQVLSSCAYFSDQDGVQHELTAEMKENIQRNEFEMAASGLRIIAVASGINTNKLVFHGL FT FGINDPPRPQVRESVQYLMTGGVRVIMITGDSVVTAISIARSLGMAIPSNDEEAIRNYA FT LTGAQLDDLDSSSLRDAVSRVVVFARTTPQHKMKIVEALQSLGDVVAMTGDGVNDAPAL FT KLADIGIAMGRQGTDVAKEAADMILTDDSFATILSAVEEGKGIFNNIKNFITFQLSTSV FT AALSLIAISSVFGFQNPLNAMQILWINILMDGPPAQSLGVESVDEDVMMKPPRPRNAPI FT ISVQLLQRVLLSAFIIVTVTIVVFRVQMQDGNVTARDTTMTFTCFVFFDMFNALACRSE FT TKSVFKLGIFSNRMFNIAVGGSLIGQALVVYASPFQRIFQTEAIGLKDVLILLACTSSV FT LWVDEIRKWYRRRKGLVRTKSNYLLRNV" FT misc_feature complement(6135..8087) FT /note="Match to PF00122, E1-E2_ATPase, E1-E2 ATPases" FT CDS join(complement(10208..10224),complement(10036..10137), FT complement(9884..9986)) FT /db_xref="SPTREMBL:O94650" FT /note="SPBC31E1.03, len:73aa, SIMILARITY: TR:P91302, C. FT elegans, ubiquitin -like protien, fasta scores, opt:354, FT E(): 9.6e-2, (66.7% identity in 72 aa overlap)" FT /gene="SPBC31E1.03" FT /product="ubiquitin-like protein" FT /protein_id="CAB39137.1" FT /translation="MIEVLCNDRLGKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLK FT KWHSVFKDNITLADYEIHDGMSLEMYYS" FT misc_feature complement(9987..10001) FT /note="ttaacaaaaaaatag, splice branch and acceptor" FT misc_feature complement(10030..10035) FT /note="gtaagg, splice donor sequence" FT misc_feature complement(10138..10152) FT /note="ctaactaaattttag, splice branch and acceptor" FT misc_feature complement(10202..10207) FT /note="gtatgt, splice donor sequence" FT CDS join(10991..11570,11701..11858,11913..12128) FT /db_xref="GOA:O94651" FT /db_xref="SPTREMBL:O94651" FT /label=SPBC31E1.04 FT /note="SPBC31E1.04, len:316, SIMILARITY:Saccharomyces FT cerevisiae, PE12_YEAST, syntaxin (T-SNARE), vacuolar, (288 FT aa), fasta scores: opt: 318, E():2.9e-12, (29.4% identity FT in 245 aa)" FT /gene="SPBC31E1.04" FT /product="syntaxin (T-SNARE), vacuolar" FT /protein_id="CAB39138.1" FT /translation="MSFVDLEQGRHKIEQNGDFPALASSIAQEIHALRGNTAAIHRYLV FT NNLTKNLHEVLEQSRELSQKVRSDLVRLANIKDTKYGEEASSFALSKLTRDFNTVLAEL FT QRVQQKCAQQESDSVAAAQAALNQDVGQHFIEEEERNVSLSNNSSGQRQPLTESKISNS FT QLEYQQRLINERQGEIENLTQGINELNEIFRDLSTIINEQGELVTNIEYNVGNTSTNTK FT NASRQLQIANEHSRKARKRSFCFLKSFAMFSSFRSQNANLYNLNSIRRNLTKHFRNRVL FT VSLYVSCSSRVWSLPYVHAFKIAYSIQFLDTDYFFT" FT misc_feature 10997..11758 FT /note="Pfam match to entry PF00804 Syntaxin, Syntaxin" FT misc_feature 11571..11576 FT /note="gtaagt, splice donor sequence" FT misc_feature 11682..11700 FT /note="ctaacaattttctttttag, splice branch and acceptor" FT misc_feature 11859..11864 FT /note="gtaatt, splice donor sequence" FT misc_feature 11898..11912 FT /note="ttaataatgggctag, splice branch and acceptor" FT CDS join(12534..12671,12722..14026) FT /db_xref="SPTREMBL:O94652" FT /label=SPBC31E1.05 FT /note="SPBC31E1.05, len:480, SIMILARITY:Saccharomyces FT cerevisiae, GLE1_YEAST, rna export factor gle1, (538 aa), FT fasta scores: opt: 712, E():9e-32, (35.9% identity in 426 FT aa)" FT /gene="SPBC31E1.05" FT /product="putative RNA export factor" FT /protein_id="CAB39139.1" FT /translation="MDTKTTPLIHAKLDEKIISSYNDANGLDIDDLWDIYNEKTRRMIH FT IISQRYKPKKQSPFPVIADENVRIFPPLHKTIDWAKKRNVEEQNLIEQSITESQRIFSE FT KQRLEQERFNRELLEKKRIEAERQRLKDEEERRKKELMEKEKKEKERIRLIEEQKHKEN FT EQRRLKQEQIDAKRKEEEAREKRMKETFKDDPEEDSNMAWSIIHKIKTEVVAPISEKKE FT LKNYCFTQKRKITPRLGQITKSNSQIMKITQLLQQTFQEARNTDPLVYKWVLNFFCKSV FT VKQAEAEVAVNPISAYPLAKVCLLLQTQNADLKDLLFARLQKNCPWVIPFWYDHGTENG FT KKKMGFKKLSDGHWEQNTTYNERQCGIFAVYAAILSLDDSLAPESWRTFSRLLNLPSPS FT QLMKSDLELGQTLCSIVSTYLDIAGQSLLRIYGRQAKKLIVCSFSEAYLGANGGGSQYG FT RLRIVGEDWMKGQGGLKFSFEP" FT misc_feature 12672..12677 FT /note="gtaagt, splice donor sequence" FT misc_feature 12706..12721 FT /note="ttaataatgttcttag, splice branch and acceptor" FT CDS join(14360..14461,14509..16920) FT /db_xref="GOA:O94653" FT /db_xref="SWISS-PROT:O94653" FT /label=SPBC31E1.06 FT /note="SPBC31E1.06, len:837, SIMILARITY:Homo sapiens, FT Q14692, kiaa0187 protein., (1282 aa), fasta scores: opt: FT 1520, E():0, (38.2% identity in 1007 aa)" FT /partial FT /gene="SPBC31E1.06" FT /product="conserved hypothetical protein" FT /protein_id="CAB39140.1" FT /translation="MDEKKGHYAKHSGPKAEKKKLKKVSDGSASNNPKAFAVASAGRMA FT RQAMRTADISQKKLHVPMVDRTPDEAPPPVIVAVMGPPGTGKSTLIKSLVRRYSKYTIS FT QITGPITVVAGKKRRITFLECPNDLSSMIDVAKIADLVLLLIDANFGFEMETMEFLNIL FT APHGMPRIMGVLTHLDLFKKTSTLREAKKRLKHRFWTELYQGAKLFYLSGVLNGRYPDR FT EILNLSRFISVMKFRPLRWRNQHPYLLADRMEDLTLPVDIEQNPKVGRKITLYGYLHGT FT NLPKHDASVHIPGVGDFVTSDVSSLEDPCPPPDADKVRRRRLSEKQKLIYGPMADIGGI FT LFDKDRVYIEVPTSNFSKDENSEAGFGERMVMQLQEAQQPLGVDGNSGLQLFSNSDAID FT TVDRESSEIDNVGRKTRRQPTGLINQELIKEDEGAFDDSDVNSADENEDVDFTGKIGAI FT NNEDESDNEEVAFADSDSDLGGQFDDEDSNLRWKEGLASKAALAYSQSGKRRRNIQKIF FT YDESLSPKDAYAEYKGESAKSSESDLVVSDDEEDFFKVSKVANESISSNHEKLMESESD FT RLSKKWENPQLLAQLKSRFITGSLLDSIEGQEEVSQDDEEGDFEDLEDEENSSDNEMEE FT SSGSSVTAENEESADEVDFQTEREENARKKEELRLRFEEEDRGDPEKKDVDWYTEEKEK FT IARQLVINREAFEDMDPESRAEIEGYRAGTYVRIVINDVPFEFVEHFDSRYPVVVGGLL FT PNEQRYGLVQVRIKRHRWHKKILKTNDPLIFSMGWRRFQSIPVYSISDSRTRNRMLKYT FT PEHMHCFGTFYGPFVAPNSGFCAVQS" FT misc_feature 14462..14467 FT /note="gtatgt, splice donor sequence" FT misc_feature 14496..14508 FT /note="ctaataagaatag, splice branch and acceptor" FT misc_feature 14647..14670 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 16744..16920 FT /note="nominal overlap SPU41410 S. pombe chromosome 2" XX SQ Sequence 16920 BP; 5679 A; 3012 C; 3104 G; 5125 T; 0 other; caccaccaag ggatttatgg ctgcgggtct ctcgaggaag acccatcaaa gtggatttgg 60 caccgagtaa tccctgtttc aaaccagaat aaccttgtct caacccttgc tctattgttt 120 caggttgttc agcgtaataa cttctggctc tggacgcaga tgcagaagca ttgcttcctc 180 tttctaaata actttcggct tgacgaagaa gagaatgcgt accggtagca ccttgcgcat 240 ttaaagacag aaagtcgttc acgtactttt cagtaaattt aattatacca tgccggaaac 300 taccgactga atggtttcct tgcaagcccc gaactggact tacaaaaatg tctttaatgc 360 ctcttcctac agtaaacatt gttcgaatcg gcactacacc attcagaact tttgagagtt 420 gattgtttcg gatatcttgc aaccaaacgt taagaagggc attaaggaat tcttctgcac 480 cagaaagtcc ataaatttgt aattgacgca agaaaacctc cgaaccttgc ataacaatca 540 agctgcctaa atcaggaagc tttccgcttc tcaatcctac cttatcagct gatttgaatt 600 taaaatcgat agttacatga gtagcattta tgcagatgga ttgaaaaaac ggtagatcag 660 gttcggtgtt taacgtttca gaggaactag gagtttgaaa agtaagaaaa cgtattaaaa 720 aatccaaagt atcttggtca atatacattt tacaaggcag aacgctaaat tccaacctca 780 attctctgtt atttagttca ggtaacggtc gcacagtaac aatttgagca ttcatgtgct 840 gattacgatt cttggaagac ggagatctgg aatctcgtcc aaaaaattta ttccaggttg 900 aggttgatag atggtcatat ataattaagt ccttcagacc aatatctaaa tcgttcagta 960 ctgcgttagg ttcattactg tcagcatata cagtaaaaga gccctgtaaa tctttaattt 1020 ctattccaat ctttttgctt tttgacttcc ccaagcgtaa atggtacgaa atagatttcg 1080 ataaataatc agcaaattca tcagaatcga ttggttcaga tacatgattg atatttaatc 1140 ttcccacttt cgaaaaagac ttgatgacta tgctttgaaa caataaggtt ttcgcttcag 1200 gagaaatttt atcattttga ctagaagaat cgcatagttt ttcgatagct gagctgatag 1260 ttgctcgagt agcttcccaa tcataaccat catgcaaatc ccagttcacg ttgatatctt 1320 taaattttac ttttaagaga taattttttc cttctacaga agattcaagc tttgaagtgc 1380 tttgacttaa gtaataatcc tcaataagct ctataccctt ttttaaggag tctcgggaac 1440 tcgattcatc ttgaagttca tcggaaatat cgataattga tgacgaacca tcatctatta 1500 caaattcctc ggacgaaaca gatgccaaat cactggcatg agtggagtgt tcgtttgaag 1560 aaatataata atcgtctacg attttaattt cttgcgaaga tatatcattg tcagaagata 1620 ctagcaccgt actctgttca gtaggattgg acttgtaatc tatgccacga ttttcaaaaa 1680 agttgtagtc aacttcggtc aaggattttg catagtcttc aatagggaga gccaaatagc 1740 aatccgatat ttcttcatct tcagttgatc ctaaatcaga caaaacttta atcaacaact 1800 cggtgctatc cgcacaagat tgtacttgtg catcgaggtt atcaacagtt atcaaggtag 1860 aaaaaacccc ttcctcagca ttgacaacta atgacaatgt caaatttttt agctgagata 1920 tttcaacata acccaaagat ttaacaacaa attcgatagt atcttttatc gagagtccag 1980 ggaaatggag ggaagagtta agaatttcac gttttaacac agaagtcttt ccttccaaaa 2040 cgctttggtt caatttgtca ataatgtata ctaccgtttc agctgccatg atacgaatgt 2100 ttaatggatt tgaagcgaca agtgcatcaa tttcaacatc aagtgacttc aaatatagta 2160 ataacttcgc ctcaagcgtc ctaggattca gacctattat tgactctcta aggtccaact 2220 taatattaaa tggaaaatca ggaaatgcta agaatggaac ttcaggatcc tgaggaaagt 2280 aggtttttaa taaatccgaa atccaagggc atgtagggta gtgctcatat ccaagatcag 2340 ttattttgac aaatatggta ttatcattag atcctgatga tcttttaagg gcatagctaa 2400 aatcagcatt taataaatac ttttcatcat ttcttaagtt ccttataaaa gaaacgactg 2460 gtaaaatttc taaagactca gataagtatt ccgcagaaac attataaagt cgtaaactca 2520 atgaaattaa ttgtgcacca gcccaacaca tgtaatgctt aattcgaatg catttgagag 2580 caaaagagct gccatcatca tttaataaag ataacgagaa tgttctggtg cggattttta 2640 atacgttttt gtaatctgaa gtcaccaact tattaggtgt aactggagtt accaaagagg 2700 aagcaatagt ttcatataaa gttttgaaaa gtcgaacgac ctccttgcta atgaaaacat 2760 taaagttatc gactgcaaaa taaaaatgct tttctggata ggaaaactcg gttggcgcag 2820 gctgaaatac tggatagtaa tgcttttcct gctgtataga aactgcaaaa ttggaagtcc 2880 cggtagtgtc atgagtttca aataaactat tagaaaggtg aaaagcagta gatgatgaca 2940 ccttggtcca atcagaattt tcacaattga ataactctga ctggcatccg atttttttta 3000 caaatatcaa tacagatgaa agagaccctg tagattttct tgtaaattgc aagtgagata 3060 ctctcaaagt aagtttgtat aatgattgag ttgaaagttc attcaatatt tgaagttgaa 3120 gctcatcaaa gagtgcaata atctcaaaac tcggttgatt catttccacc tcaaagggtt 3180 tgggcgcctc aaaggtttct tgaattgacc gtttaaatgg atctgaattt gacaggaaat 3240 tttgcagatt ggatatatca ttagtaaact ccacgaacga ttcttgaagt aataaggcct 3300 ttccaagagg taaagaaatt tctcccccag gaatgtgtat gaataaggac actattcgtt 3360 caagcttttt aaaaatcagc gagcatggtg attgcagact tttaccaaaa gacaccagtg 3420 taacagaatc cataaaaata tctaaatcag agaaagtcat cgttagttca tcaatatcat 3480 tattgtcatt aaagatttga atttgattaa ttttcatttc aaaaacagct ccatttgagg 3540 ggatggtatg tagcaattca tcaacatcat tatgcataga aaaatcctga gattcgggga 3600 gtttacagtg ggtaaggatt tgtacagaag atatgtggat atcaagttgt aacatagtaa 3660 gaggcggttt atttgaagaa aatccagggg tggaaggagc aggtaaaaaa tacccgtaga 3720 gatataacag aaacgcaatg acttgtgagt taatattaag aacacatgaa gggacgtgag 3780 actcaataac caataagtta gaggactcaa tactgaaaac aagcctaaat tcgccagaac 3840 attgaaaaat taaccagcta gtccttgttt ttttctgagt aacagaatcg tggacagagt 3900 aaaatgattc ttcttctcta tttgagctta ttattgatga ttcatttagt gtgacctgtg 3960 atggcgatag atcattctca aagccctcta ctatactaga agaggaatcc atcgatacaa 4020 atgactcttc agatcgagga ggagaagatg atcctgcgtt actcttgctt atgggccgaa 4080 ccatggatac tgttatgcca ttaatattga gtaatcgaga tgtagagtta cttttaaccg 4140 acgaaaagct cataaaatct agttttgctt ccaacctagt agacatattt gcagtcccta 4200 tgtaaactaa agcgtcttgc ataaggacag aggtagatgc caaacatttt tgcaatatac 4260 tatccaataa attatgtgaa gagccatcaa tttcagattc taatccctcg ttgatcacct 4320 catcttcaaa gtcttgcgat gctgttttat tcatatattc aacaacatta tccaatatat 4380 caagctgtga tggagcatcc tcatatgtag ttgagcttaa ggattcatca ggaggtacta 4440 gttcaatttc caaacttaag ccattgactt ctaattcaac gtttaaattt acaatatcag 4500 taacattcag tcttaaaatt aatctcctca acgttccttt agtaaaatta atcattggaa 4560 gagacaccac tgcattcaga aaggaacaat ttagctgtaa attggacaac attaggaccc 4620 cttttgaaaa aaggatgtct atatcctcag gctgcacatt ttctaaaaga atggatccaa 4680 taagctttct caaggcaaat gctaatagtc gacgttgaac agtccttgat aacgccgccg 4740 tccagagcca agaggaagaa tttttaagcc atgagggtaa cctcatcatc ctcctttgct 4800 taattgaatt tcgggttcaa atttgtgtct atctcacact tatttgcctc attcagattt 4860 tggtagtggg acgacaaata tgcgacgata gcgttgatgt acacgaaata aaaacaataa 4920 tgccaacatc ttgtgacaat ctaatgaatg gaaaatttac tcattgaaga ccaaacgtta 4980 atgacaagaa aaattactta aggcgagtcg gtcacatgaa tcaactgttc aataaacatg 5040 tataaactga ctttgaaaca gcacaagata taatgataca aaaacaattt agagcaacgc 5100 ggctgtttaa tctcgtataa tttttctttt gcattaatta aatgatgaaa agcgacaacc 5160 gattttaaaa cctttaattt tgataagcct ttattatttg tttctatata gaaactacaa 5220 ctaatatgtt ttagattaac ctttagtcag aataaaatca tataatttgg ttttaagact 5280 aaccagtgag gactgcatca aggtgatgaa taataaagcc tgaacatact aagtgcttga 5340 aaattaacca agatacagga ttttcgaaaa aaaaaactga aatgaaaagt aacgatgaaa 5400 gcttttaaac gaatggtata gactcgagta cttcatggca gagaagcaaa ccccttttgt 5460 gccataagaa taagaaacca gcggaaagct aataatcatt agtcaaaaaa ttaataaaat 5520 acaggaattt tttaaataca tatagcattg gaattttgta ttcacaaaat aatagtaaag 5580 ctccttaagt aattttaaac ttagcacaaa gatatgcaac ttgttaccgc aaaacgtatc 5640 atttatacat tccttagcag ataattgctc tttgttctaa caagaccttt acggcgacga 5700 taccatttgc gaatttcgtc aacccagaga acagaactgg tacaagctaa caatattaga 5760 acatctttaa gtccaatagc ttcagtttga aaaatacgct gaaaggggga agcataaact 5820 accaaggctt gaccaattaa tgagcctccg actgcgatat tgaacattct attagagaaa 5880 ataccaagct tgaaaaccga ttttgtttcg gaacgacatg ctaaagcatt aaacatatcg 5940 aagaaaacaa aacaagtaaa agtcatagta gtatctctag ctgttacatt gccgtcttgc 6000 atttgtactc taaataccac aatagtgaca gtaactatta taaatgcact gagtaaaaca 6060 cgttgtaaaa gctgaactga tataatagga gcattccttg gacgaggagg tttcatcatc 6120 acgtcttcat caactgactc tactccgaga ctttgtgcag ggggtccatc cattagaata 6180 ttgatccaaa gaatttgcat ggcattgagg ggattttgga agccgaaaac agacgaaatg 6240 gcgattaatg aaagagcagc cacactcgta gacaattgga aagtaatgaa atttttaatg 6300 ttattgaata ttcccttacc ttcctcaaca gcactcaaaa tagtcgcaaa agaatcatcg 6360 gtaagaatca tatcagccgc ttctttggca acatccgtac cttgtcgacc cattgctata 6420 ccaatgtctg caagtttgag ggcaggtgca tcgtttacgc catcaccagt catagccaca 6480 acatcaccaa ggctttgaag ggcttcaaca attttcattt tatgttgagg tgtagttctt 6540 gcaaagacta ctacacggga aacggcatca cgtaaagagg aagaatcaag atcatctaac 6600 tgagctccag tcagcgcata gttacgaatt gcttcttcgt cattactagg aatagccata 6660 cccaaacttc tagcgatgct tattgcagta acaacgctat cacctgtaat cataataact 6720 cgaacccctc cagtcattag atattgtaca ctttcgcgga cttggggtcg agggggatcg 6780 ttgatgccaa aaagaccatg aaatacaagt ttattggtat ttataccact ggcaacagct 6840 ataattctga gtcccgaagc agccatttca aactcgttac gttgaatgtt ctccttcatt 6900 tccgcagtaa gctcgtgttg aacaccatcc tgatcgctaa aatatgcaca acttgacagc 6960 acttgttcag tggctccctt catgaaattc attttgctag agttgtattg gacagcaaca 7020 gacatgtatt tgcgctcact gctaaatgaa acctcagata tacgactgta cgtttcacga 7080 ggatccttca acccgaatcg ctcagaacat tcgattaaag cgacatcaac tggaaatcca 7140 gcccagggac aagtagtgtc caaaatagaa tcagctttgt tatgaacttt actattgttg 7200 cataaagcag cagctagaag tgccttttcg ataccaacag tgcgcctaac acttagctct 7260 atatgttcac tttctggtaa ggagaaagct gctaacattc cacatgtata aattttagta 7320 actgtcatat ggttcattgt tagagtacca gtcttgtccg aacaaataac gttaacactt 7380 cccaaagttt caacactggg taaccgacgg ataatagccc tcttcttaga catgcgtaac 7440 acgcctaaag caagggtgac tgttacgata attggaagac cttcaggaat agcggcgaca 7500 gccaaactaa ctccaattgt aagcatttct aaccaatttt taccttggaa aaaaccaaca 7560 agcacaatga cggcaatacc aattaaagag attaaagaga gttgcttgcc taagtcatcc 7620 atactgttct gcaatggcgt ctttggtttt tcagtttgct gcatagtaag gaaaacacga 7680 ccgaattccg tatcagatcc agtagcgaca actattcctc tcccatgacc atgacgaacg 7740 agggtaccca taaaagctat gttatttctt tcagtaagag atatgtttga agatatagct 7800 tcgctactct tctttcgtgg cgagttttcc cctgttagat ttgattcatc aatttccaac 7860 tcggtagctt caacgattcg cagatcagca ggaacacgat caccaatttg taagataact 7920 aaatctccag gaaccaattt actagcaaca atatgctccg tttttccgga ccggataaca 7980 ttgcaataat gaggtacaag gttattcagc gcctttaaag attgctcaga tcgatactct 8040 tgaacaaatc caacagtcac gacaataact atagctaaag cgatgctaat agcatcatct 8100 atattaccca atgtcaccga aattgcagaa cttgcaaaaa gcaaaagaat taggggatct 8160 ttaacaaatt gcttaagaaa ttgaaccacc atgttttctt catcttctac cttcaaatca 8220 ttatctccat gaaccttgtt acgtcgcgtg atctcctgga gactagagag gccattatac 8280 atgtcagttt ctaaatccgc acaagtttgc tcaacactga atgcatcata ttgaacactc 8340 attatggaac tgtttcatac aaaaacaaaa acgagaaaaa gtagtaaaag aaagttgggg 8400 taatgtaaaa ataaaaaatc ttgtaaacaa aaaaaaaaaa aagacactgc tattcgatac 8460 tttctaaaac gccagtataa aagcaagtat ttaagatgta aaggattcct aaatgctcag 8520 cacaaagaat atactttcca atgttgaaaa ttaagagata gatgaattta ccaacaatta 8580 atctagacaa tgaacaatat ccacttatca ctcataaaat ccaaagaaat gttgaaagga 8640 acccgtgaga aataaaaaaa gaatttataa actgtccaag ctcactgtta gaacgtttca 8700 aaagaaagtc ctagaaaatc ggacttatga acctagcacg tctctggtta aataccaaca 8760 gacccagcat acaaaaacgc aaatcctggc ttagaacttt gaacaacact tgtttatatt 8820 ctcaaagaat aaagcagtac ttttgtttcg accttttaga ctggtaagct tagaccaacg 8880 aaggtgatcg tctggatgac aagttgggaa aggttagctt gagtaaagat ggaggcttag 8940 cagatacttc tgagtcaagc tattacaact ccaaaatgta aaagaagtat aatcaattca 9000 gagaatagtt ttaaaaaaat tgaatactcc cgtagatgat tttttggtaa ccaatacata 9060 tctgaaattt tttcatgtta atgaattttc tgattttttg aaaagcaggt caattgagtt 9120 tcatcattac taaaaatgtt taaaacctgt ttcaaagaat tattttctta aaaaaaaaaa 9180 aaaatattac attcagaaga aagcagggct aagaaataaa acagtggcgt aacattataa 9240 agctttatac taatataatt cggaatccct cttttccttg ataatttgtt tcatcttcct 9300 tggtatgatg aataaatacg aagcattgaa gacataattt cataaacggt catgaaccta 9360 ggaaattggt cctacccatg acaagatata agccttgtaa atttaatggt aaatttgaat 9420 ttgaaaaatc cattcatatt aagaataaac atactggttt ccatttttaa agcggttaca 9480 cttgactaga agcgtaaaag gcttgatgaa caactattga tatggcagct agaaaacaat 9540 attattatca ttgtctagct gacttatatc acaatttggg gaggtaagca atttgaaaga 9600 taaaaaagta tccaaattat tagactcaaa aagatagcat ttcaacgaaa aatcattatt 9660 tatttagtag tttgaacagg taaatacaaa accttcgaag gtgaattgaa aaaaattgaa 9720 aataataaac caaatttcct ttgtaaaaaa cttaacaaat ctgctcaaca atataactct 9780 atccatcaga ttttcattaa acaagtttaa agtcaatttg ctactttcgt taacataaaa 9840 cttccaagta tattattttt taagaaaata aaagtttcgc tttttaagaa taatacatct 9900 ccaagctcat tccatcgtgt atttcatagt cggctaatgt gatgttatcc ttaaagacgc 9960 tgtgccattt ttttaagacg atccttctat ttttttgtta acattttttt aacaaatgat 10020 gaatttaatc cttaccttgg atctgtaccg gtctgagctg caacaagttt tttaaaatct 10080 ccaactgtgt catcaggcat acattttacg cgtacttttt tccctagacg atcgttacta 10140 aaatttagtt agagctttat gttttccaat ttcaatgctc ccagaaagta aaataagtca 10200 aacataccat aaaacttcga tcatattgaa tgcaaaagta atggagagtt tagttaccag 10260 tttgtcttca ttaaatgtta tttaacttta gtctcgttaa taatcgttaa cgtcaggaaa 10320 gtgcgttagt tttcctgccg gtgaatttta taagaatgaa ttcagtagtg catgttattg 10380 ttttatgtgt ttcattgtat agaatccact ataaatccat ttaatctata ctcgttgagt 10440 acctttgtga agtatcaatt gcatcagcaa cataatttga tgcgagcgta tgaatggccc 10500 cttataagta ttctgagtat ttgtgatttt ttatttgaaa ttcaaattat caatatttca 10560 attaattcct tttaatcgaa tgtgtcctat attagatttc agccatttta gatgtttact 10620 aaatagcgac taataataac ctattgtaag attgccttct ttcgcactat acataaataa 10680 agaactttag agtacttacg aaaggcgttc gctccggttt gctgggtctt actaattcct 10740 attaggtatg agccttgctc ataagatttg cattcttctt ccaactacta aaacacttga 10800 caattgtcaa ggtttctacg aacaaaagaa ttgtaacctt agcaaggtga ctaattattg 10860 ctgaaaaact ttgaatcttt gttttcagac tcaattcaag ctaattggcc aattttttaa 10920 ttatattgac tttcttagta cctttgcaaa tactgttgga ttgctttgat tcaatttttt 10980 tgttgctata atgtcttttg ttgacttgga gcaaggaaga cataagatcg agcaaaatgg 11040 tgactttcca gccttagcga gcagtattgc tcaggaaatt catgctctac gaggaaacac 11100 agcagcaatt catagatatt tggtaaacaa ccttacgaaa aacttacatg aggttttaga 11160 gcaatcccgt gaactatctc aaaaggttcg ttcagacttg gtaaggcttg ccaacatcaa 11220 agatacaaaa tatggagaag aagcgtcgtc cttcgcctta tcaaagctta cccgagattt 11280 taatacggta ttggctgagc ttcaaagagt acaacagaaa tgcgctcaac aagagtctga 11340 ttctgtagcc gcagcacaag ctgcacttaa tcaagacgtc ggacaacatt ttattgaaga 11400 ggaagagcgt aatgtgtctc tatcaaataa ttcttctggt caacgacagc cgttgactga 11460 gtcaaagatt tcaaattccc aactagaata tcagcaaagg cttattaatg aacgacaagg 11520 tgaaatcgaa aatttgactc aaggaataaa tgaactcaat gaaatctttc gtaagtatca 11580 gctagttgac attttttttt tttctgttta cgtgatggag ttaggagtaa tgctaaatac 11640 ttgacctaaa atattttcgc ttttataaat ttaaagtttt actaacaatt ttctttttag 11700 gcgatctaag caccattatt aatgaacagg gtgaactagt taccaacatt gaatacaacg 11760 tcggaaacac atcgacaaac acgaaaaatg cttcaaggca gttgcagatt gccaacgagc 11820 attcaagaaa ggctagaaaa agaagctttt gctttcttgt aattctagtt gttatactgg 11880 gggtaatatt aacagcttta ataatgggct agaaatcttt tgctatgttt tcctcattca 11940 ggtcccaaaa tgcaaatctt tataatttaa attcgataag aagaaatctt acaaaacatt 12000 ttcggaatcg tgtgttagta tcgttatacg tgagttgctc ttcacgtgtt tggtcattac 12060 cgtatgttca tgcattcaag atagcgtatt ctattcaatt ccttgataca gattatttct 12120 ttacttgatt tattttaatg acctagagga agtcctccaa tatcagttaa tccattttag 12180 ctatagacga agtcaaatct ctcgctttgc ctgcaattgt ttatattgca ttcttactta 12240 ttgtattttt ttgcaacttc attgggctag ttatactttg atttttaatt aatgatactt 12300 gtttatgtta atatatactt atgtctcata gtttggatga ttgcttgttc tgttttttta 12360 attataattt aaaccgccta atacaatcta ataacctaag acttttatag ttttgacact 12420 tgtaatgtta attaaagcaa gcatcaagtt tcgatcaatt tgagtttcgc atagtgaatg 12480 tccattccaa atcgtccttt taccaatttc ctcaatttca ggaaatttca gttatggata 12540 caaaaacaac acctttaatc catgctaaac tcgatgaaaa aataatatct agttataacg 12600 atgctaacgg tcttgacatt gatgatttat gggatatata taatgaaaaa acacggcgaa 12660 tgattcacat agtaagtatt cgattttcta ttaatattat ttttattaat aatgttctta 12720 gatatcacaa aggtataagc ctaaaaaaca atctccattt cctgtaattg ccgatgaaaa 12780 tgtgcgtatt ttcccaccgt tgcataaaac gatagattgg gcaaagaaga ggaatgttga 12840 agagcaaaat ttaattgaac aaagtataac tgaatctcaa agaatttttt cagagaaaca 12900 aaggttagaa caggaacgat ttaatcgtga attgttggaa aaaaagcgta tcgaagcaga 12960 acgccaaaga ctgaaagatg aagaggagag aagaaaaaaa gaattgatgg aaaaggaaaa 13020 aaaagaaaaa gaaagaatca ggctgataga agagcaaaaa cacaaggaga atgaacaaag 13080 gcgattaaag caggagcaaa ttgacgctaa aaggaaagaa gaagaagctc gggaaaagag 13140 aatgaaggaa acatttaaag acgatcctga agaggactca aatatggctt ggtccatcat 13200 tcataagatt aagactgaag tagtagcccc gatttctgag aaaaaagagc tcaaaaacta 13260 ttgctttact caaaaacgga aaattacacc acgtttaggt caaattacta aaagcaattc 13320 tcaaatcatg aagattacac agctactgca acagacattc caagaggcca gaaatacgga 13380 tcccttggtc tataaatggg ttttgaattt tttctgcaaa tctgtggtga agcaagcaga 13440 agcagaagtt gctgtaaacc caattagcgc ttatcctttg gcaaaagttt gccttttatt 13500 acaaacacaa aacgccgacc ttaaagatct tctttttgct cgcctacaga agaactgtcc 13560 ttgggttata ccgttttggt atgatcacgg tacggagaat ggtaaaaaga aaatgggatt 13620 taaaaagcta tctgatggcc attgggaaca aaatactaca tacaacgagc gacagtgcgg 13680 aatttttgca gtttacgctg ctatactttc tttagacgat agcttagctc ccgagagctg 13740 gagaacattt tctcgattgc tgaatcttcc atccccttca caattaatga aatcggactt 13800 ggaactaggg caaactcttt gctctatcgt ctcaacgtat cttgatattg ctggtcaatc 13860 tctattacga atatatggaa gacaagcaaa aaaacttatt gtttgttctt ttagtgaagc 13920 ttatctagga gcgaacggag gtggtagtca gtatggtcgt cttcgaattg ttggagaaga 13980 ctggatgaag ggtcaaggag ggctcaaatt ctcctttgag ccttaaattt ttttgatggg 14040 ttttcaacct atttactttt gatttataat atacttacgg tagaaattat agtcgttagg 14100 atgtgtacta gataattgtt tcaatttaat tcaaccatta cgatttaaat gatcctggct 14160 ttgatgtcag tttcgctgat taataccata tttacttttg cattaaataa agataatttg 14220 actcaagaaa tgcattttcg ctacagacac tgctattcaa aatttgttat atcatgttac 14280 cttgatgtga cttaaagtgg attttttcac ctactcaact ttgagccttt acaaatatct 14340 agcttgttcc atagttgtca tggacgagaa gaagggccat tatgcaaaac attctgggcc 14400 aaaagctgaa aaaaagaagc tcaaaaaggt gtccgatggt agtgcttcaa acaatcctaa 14460 ggtatgttga gaacttttgt aaataatcaa attggctaat aagaataggc atttgcggta 14520 gcatctgcag ggcgcatggc taggcaagct atgcgtacgg cggatatttc acaaaagaag 14580 cttcacgtgc caatggtgga tcgcacaccg gatgaagctc cgccaccagt gattgttgcc 14640 gttatggggc ctcctggcac tggaaaatct actttgataa aatctttagt acgtagatat 14700 tctaaataca ctatttctca gattactggg ccgattacgg tggttgcagg aaagaagaga 14760 agaatcacgt ttttggagtg tcctaacgat ctcagctcta tgattgacgt agccaagatt 14820 gcagaccttg ttctcctttt aattgacgcg aattttggat tcgaaatgga aactatggag 14880 tttttgaaca tattagcacc gcatggtatg cctcgcatca tgggtgtatt aacacatctc 14940 gatttattta agaagacttc tacattgcga gaggctaaaa aacgtttgaa acacagattt 15000 tggactgaac tctatcaagg tgctaaattg ttctacttgt ccggcgtttt gaacggacga 15060 tatcctgata gggaaatcct caatttatct cgattcataa gtgtcatgaa atttaggcct 15120 ttgcgatggc gcaaccaaca tccctattta ctagctgatc gtatggaaga ccttactttg 15180 cctgttgaca ttgaacaaaa tccgaaagtt gggcggaaaa tcacgttata tggttatttg 15240 catggcacaa atcttcctaa gcacgatgcc tctgtacata tccctggtgt tggtgatttt 15300 gtaacgtctg atgtttcttc cctagaagat ccttgcccgc ctcccgatgc cgacaaagtc 15360 cgtcgccggc gactatcaga aaaacaaaaa ttaatctatg gccctatggc cgatattggt 15420 ggtattttgt ttgacaaaga tagggtttac attgaggtac caacttcaaa tttttctaaa 15480 gatgaaaatt ctgaagctgg ctttggagag cgtatggtta tgcaacttca agaagcccaa 15540 cagcctctgg gagtcgatgg taatagtgga ttacaacttt ttagcaattc agatgctatt 15600 gatacggtcg atagagaatc aagtgaaatt gataatgttg gccgtaaaac ccgtcgccaa 15660 ccaactggac ttattaatca agaactaatt aaggaagatg aaggggcatt tgatgattct 15720 gatgttaatt ctgctgatga aaacgaagac gtcgacttta caggaaaaat tggtgctata 15780 aataacgagg atgaaagtga caacgaagaa gttgcatttg ctgatagtga ttccgattta 15840 ggaggtcaat ttgatgatga agattctaat ttaagatgga aagaaggact tgcttctaaa 15900 gctgctttgg catactctca atctggtaaa aggagaagaa atattcaaaa aattttttac 15960 gatgaatccc tttcccctaa agacgcgtat gctgaatata aaggtgaatc tgcaaaatct 16020 agcgaaagcg atttggttgt ttcagacgac gaggaagact tttttaaagt gtcaaaggta 16080 gcaaatgaat ctatatcttc gaatcatgag aagcttatgg aatcggaatc tgaccgtctc 16140 tcgaaaaaat gggagaatcc acagctgtta gcacaactga agtcgaggtt tataaccggt 16200 agtctccttg attctataga gggacaggaa gaggtttctc aagatgatga agaaggtgat 16260 tttgaggatc ttgaagatga agaaaattct agcgataatg aaatggagga atcatcaggc 16320 tcttctgtaa ctgctgaaaa tgaggaatct gctgatgaag tcgattttca aacagaacgt 16380 gaagaaaatg ctcgtaaaaa ggaggagcta cgtcttcgtt ttgaagagga agatcgtgga 16440 gatccagaaa agaaagacgt tgactggtat acggaagaaa aggaaaaaat tgcacgtcag 16500 cttgttataa atcgagaagc ttttgaggat atggatcctg aaagtcgagc tgaaatcgaa 16560 ggctatagag caggcactta tgtacgtatc gtaataaacg acgttccgtt tgaatttgtt 16620 gagcactttg attctcgcta tcccgtagtt gttggtggat tactacctaa tgagcaacgg 16680 tatggattag ttcaagttcg tattaagcgt caccgttggc acaaaaaaat acttaaaacg 16740 aatgatcctc tgatttttag tatgggttgg agaagatttc agtcgatacc tgtatattca 16800 attagtgact cgcgtactcg aaataggatg ttgaaatata ctccagaaca catgcattgt 16860 ttcggtacct tttatggacc cttcgtcgct cccaacagcg gtttttgtgc ggtccaatct 16920 //