ID SPBC31A8 standard; DNA; FUN; 6643 BP. XX AC AL035584; XX SV AL035584.1 XX DT 01-MAR-1999 (Rel. 59, Created) DT 07-MAR-2002 (Rel. 71, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c31A8. XX KW cwl1; gene free region; low-complexity. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6643 RA Wood V., Rajandream M.A., Barrell B.G., Volckaert G.; RT ; RL Submitted (01-MAR-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Katholieke Universiteit Leuven, Laboratory of Gene RL Technology, Kardinaal Mercierlaan 92, B-3001 Leuven, Belgium XX DR GOA; P53694; P53694. DR SWISS-PROT; P53694; CWL1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c31A8 is overlapped at the 5' by cosmid c651 and CC at the 3' by cosmid c3D6, EMBL entry SPBC3D6, CC accession number Z95620. XX FH Key Location/Qualifiers FH FT source 1..6643 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c31A8" FT /map="IIR" FT misc_feature complement(1..190) FT /note="SPBC651.12c hypothetical proteincompletely contained FT within c651." FT misc_feature 1..1490 FT /note="nominal overlap with cosmid SPBC651, EM:AL035570 S. FT pombe chromosome 2" FT CDS complement(1028..1954) FT /db_xref="GOA:P53694" FT /db_xref="SWISS-PROT:P53694" FT /note="SPBC31A8.01c, len:309" FT /gene="cwl1" FT /gene="SPBC31A8.01c" FT /product="cwl1 protein." FT /gene="SPBC651.13c" FT /protein_id="CAB37626.1" FT /translation="MSEQHSLNPFESGSVTASDVAAAKSGAEDLVNTLTAHTVHPSTEL FT PSATSFPSALPNSENPVIQNISSSSSEPHHTSQSTPGETSSPVCPVSGAHGGADKKCPA FT LEAGCPFTNTTKQNVDPEISNALWSVLTWKNTSCSFSTLMSILALVYVPSWINLPRLFF FT RTFRYVFLITSIIEFGGLFASNGKRGVLSHFRSSYITCDSKALDRIVNSIVDIFNVMLI FT QFQRILFAESPILTFTASVAAFIEFFLSGFLSYKSLFVWNVLFAFILPRLYVCNERSIK FT HLVASLERSGDKLKKQATETINTTVNK" FT misc_feature 1955..6643 FT /note="putative gene free region; low complexity" FT misc_feature 6444..6643 FT /note="nominal overlap with cosmid SPBC3D6, EM:Z95620 FT S.pombe chromosome 2" FT misc_feature 6446..6643 FT /note="previously annotated as SPBC31A8.02, SPBC3D6.01 FT partial, but has no met" XX SQ Sequence 6643 BP; 2316 A; 1068 C; 1295 G; 1964 T; 0 other; gattggaaga cttgccattg aacgattgat cctcaacatc ttcaattgct tccatagaca 60 gcgccattct atgaagtttc tcctttttcg cggaattaaa tccaaaagct ttgctacctg 120 ctgaagctga ggtagcttca ttttgagatg cgctttttcg aggattgtat ttgactttct 180 tggaactcat gtataatagc gttgcggcat gataaattga caataaattg aatttcaata 240 atcctaatat atggatacaa aggaaatttt gagcttaaac gtaaaaagct ggcacaacat 300 tgatataagt gaatgacttg tggtgagagc agacaacgat catgcgagtg taatcagcac 360 tttactgtat gatcggctgt attaaatacg aagtagtgga aaaaaaataa taatttttgg 420 ttaacaaata ctttagtcat ttggttaaac aaaatagatt ttttcgtcca cagacccatt 480 ttagttatgg aaaatatata tatatatttt ttttttcgaa aagagcagca ataaggaata 540 cgaggaggac ctacaaaaca acacggagta attacgagat taaattacga ataataagta 600 cgttgggtat taaacgaagc agagaaaata taataaaata gcatcatatt gagcaaagca 660 acaagaactt caaccacact gcaatagttt gcaactcaga agagaaatga gagattgagc 720 cgaacctgtt acatttacaa tatatcgaga tgagaaagac gtatttatga aaataaacac 780 tcatagtcat aagcaaaata attgagaaaa cgcattcaat ctcctaattc aagtcataat 840 acaacacttt tgaaacgcaa cacaacacag caacacattt tcaaagttga aaacaaaacc 900 atggttatta cccttgcatt tttttgcaaa agatactatt gaaaccttaa aataaacgag 960 gaagcgaacc aaataaaaat caagcgcaac ctgacagaaa caatcaaaca aattatcccg 1020 cagaggttta cttgttaaca gtggtgttta tagtctcagt agcttgcttt ttaagtttat 1080 caccagatct ctcaagggaa gcaaccaaat gcttgataga gcgctcattg catacataca 1140 atctgggaag gatgaaggca aacaaaacat tccaaacaaa gagagacttg taggaaagga 1200 agccactaag gaaaaactca ataaaagcag caacagaggc agtaaaggtc aaaatagggc 1260 tctcagcaaa caagatacgt tggaattgaa tcaacataac gttgaaaatg tcgacaattg 1320 agttgacaat cctatcaaga gccttggagt cgcaggtgat gtaggaggaa cggaagtgag 1380 acaagacacc acgcttaccg ttggaagcga aaagaccacc gaattcaatg atggaggtaa 1440 tcaaaaagac ataacggaaa gttctgaaaa agaggcgagg aagattgatc caagaaggga 1500 cataaacgag agcaagaata ctcatcaagg tagagaaact gcaagaggtg ttcttccaag 1560 tcaacacaga ccacaaagca ttagagatct caggatcgac gttttgctta gtagtgttgg 1620 taaaagggca accggcctcc aaagcagggc attttttgtc ggcacctccg tgagcgccgg 1680 aaactgggca gacgggactg cttgtctcgc caggtgtgga ttgagaggtg tggtggggct 1740 cggaagagga agaagagatg ttttgaataa cgggattctc agagttgggg agagcagagg 1800 gaaatgaagt ggcagagggc aattcggtgg aagggtgaac ggtgtgagca gtcaaagtat 1860 ttacaaggtc ctcagcaccg gatttagcag cggcgacatc ggaagcagtg acggaaccgg 1920 attcaaaggg gtttaatgaa tgttgttcag acatgatttg cgtgttatat tcaattttaa 1980 aataacaact taaggtaaaa cagttactaa gaatcaaaag aagccttaaa aaaaaagaga 2040 aaccttttaa actaaaaaag ggtgcaagtg tttttttttt aagatatagt aaaaaataga 2100 gaaaatttaa agaaaactaa ttctgaaaag tgccgactaa agaaaatagc tagtaaagga 2160 cgacttttcg gaagagcgaa atactttgta acaaagactt aaaaaagtga ataaaagatt 2220 tttggatttt gctgtaggat aaataaaatg tatatgtatt aaatccaaag ccaagttcca 2280 aaagggttaa cttttaaggg agatattgat cgaaagaaaa aataaaagta gaatgggtaa 2340 atacaaaaag agaccttcac gagttgttga gaggaaagtc aatatgtttc acttacgaaa 2400 ctgttagaaa gttgttagtt ggaaagcttt gcttttatat ggtaaaagtc aagtcggtgg 2460 ttggtggtta gatagcagtc atagcgttga tcatggaatg acgttgttct tggctcaata 2520 agtctgtgtc agcggcgact atagcgatca tgcttataat cggtttacag caggctaatg 2580 aaagagaatt ttggatggta aaagaaaaac gaagaatagg aagaagatgg gcgggaaaag 2640 atgggttaaa tggtatgaaa agatgggtga aatggtatga aaaagaagga aatgaataga 2700 gaaacaatga aaatggatga aaaacgaaag aacgagcgaa aacggatgac ttttgcaaaa 2760 gctagagata ttgaaaaaag agtaaataaa gggaaaacaa tgattaagtg caggaaaggc 2820 tacaagaggc gaaaaaaaag tgagaaatag ataaataaat tcgtgtagga tagtttgttg 2880 aaaggagagc gaagtatgga gtttgattgt aaaatgtaaa ggagaaaggg ataagagtga 2940 tgaaaaggaa aaggatacaa ctgaataaag aaaggattaa agattgaaaa aggaaaggaa 3000 tagtaacggt gagaaatgat gaatcatcca caagtgagta aaacacgaag aaatttgttg 3060 tttaaaaaat tatttaatat ttgaggaatt gaaatcaatt aaaactttga agaaacaaaa 3120 ttcattcaaa cttctgaagc cttttttttt tttttttttt tttttggaaa tttttttgac 3180 aaaaaattta tttcttaaga atcaaagcaa atgtatttca aaaaaaaata tatatatata 3240 tatgatgaaa taaataagac catacctttt ctacatttag taattgattt atttcagttt 3300 acgttaatta atttaatgta attttatttc gaatttttta ttttttttat tttttcacga 3360 attgaattga attgaataga ttttcttttt ataatttaat tttttttgta caaatcagtt 3420 tgtattgaaa ttcttcatca tctcattatg cgttgtgtgt ctgtttattt acacccactt 3480 aaaaattgca agcataccgt atgatgaaca agaaggcttt tcttgccttt tcgtgaaaag 3540 ggagggagaa agagaaaacg tatttaataa attggacatc tcatcgtatg ctagtatatg 3600 aaattatgaa ttgaaatgaa atgtatggaa ttgcattgcg ctggactgaa ttgcatagaa 3660 gtggactgca ttgcatagaa gtgaattaca ttgtactgaa agaattgtgt tgtatcgtat 3720 tcaaagtaat gatgtatcat cctttcctct ttcctctcta ctctactcta ctgaaacggt 3780 cgggttcttc tctcctttag caaatccttt actgcactct gctgtacggt cttgttccac 3840 agtatgatgt ttaatgaatt gttggacaaa tctcgttacc atcaccagca tgagctggtc 3900 gcgactgccg cttatcggat gggatgagat gggagtatgg catcggtaca tgtataaggc 3960 aagggagtgc agtacgaaaa agaaggatta cgaaggagga aaaaggggaa acgaaaagag 4020 caaaaagtaa aattagacac agcaagaatg cttagctagt caaatgggtc aaatatggaa 4080 agagatggaa attttctgaa taacgaggaa aggggtgaaa gaatttgttt cggtatgtga 4140 tatagagaga aggagagaag tgcaaggaaa gccaaagaaa tgcaaggaaa gtcaaggaga 4200 gtaaaacaag gtgaaagaag agaaaaagag tactatggat cagtgagagt gaaagaggtt 4260 tattgaaagg gagcaaagtg cggtatatgg tggaagaagg tagaacgaga gagacagatg 4320 ggcagagtgg gagcagtaaa agctttaagg ttatttaggg attgttgtag cgggacaaga 4380 gagaaagcaa aggaggaaat gaaaagcaac agataatagg aataaaaagg atgaatagga 4440 atgaaaaata aaataaaatt aaagtaaata aaaagtgaag taaaacaaaa taaaatggtt 4500 aatgaaatct atttgtaatc aattctcgtt caaccatttt ttctgaattc attcattttt 4560 tgttttattt gtgtactatc gtgtggaagt atagaaaaaa aagtaggaaa tggttttatt 4620 gatgatagaa aaaaatatta aaaaaaaaaa aaaagaatta aatagtatta atttatgcat 4680 catatacaaa atgagtctga taaatttgtt tacagtgaat tttcatttca aattttttat 4740 ttatttattt atttattttt tttattatat tcaagaatat atttcattta gaatttttat 4800 tgtttttaca tatttactac tgaagtttca tttctttctt agttggtttc tttttcattt 4860 ctttctaatt tttactttat ttatttactt tatattttct atttatttat tttttatttt 4920 ttatttttat tttcatttat tttttacttt ttcctcctct ttccattcct ctccttcttc 4980 ttctctctct tctcttctct ctctcccttc ctgcctctcg ctgctctcct atcactcttt 5040 tctgccggtc tcgtctcgat actcgttacc cgaaaaaact cggctggtat gttacttgca 5100 aactgccgtc taccaatcag catcgtcgct tctttatggg tcacaaaggt gtgctggcat 5160 actggatgca tacaaaacgt tatccaacac tcttaaagtt gtgcaatgcg ccatgcaata 5220 ttcgcttacc gaacatacgc tggactacca tacataatgg atggaaactt gctttaaggt 5280 atgtgcatgg cgagacgaga ggtgggagag agagagagag agagagagta atgatagaga 5340 aagacaaaga gagagaaata gatagagtgg tagaattaac acaaagagga atgacacaca 5400 cacacacaca cagagtagtg attaagacac aaaaagtaaa aatcgacagt tccaacttac 5460 agctcactac actaaactac ctgcacaatc aaacccattt taccgctggt gatgctcaac 5520 ttctgtaatg cctaatgccc tacccctacc tctgtaatgc ttcttcctct gtaatccact 5580 tccgctgtaa tccgctgctg tcatgacgat ttttcgacaa aacaaaacat tcattcgtct 5640 acctcctccg tcaacattcg tctacatttc tctgccaccc gtcatcatga ttcacccttc 5700 cacctgctgc ttaccttaat ttcctttcta ccgtcctttg cataccgtcg ccgatgctgc 5760 tgctggctct actgtctgcc tcctctattc acggtagtca agaaggaaaa tttcctcttc 5820 tccttcccgg ctcttgtcgt cacaaatcaa ccctattgct attgtggatt tcttccatcc 5880 tgccacgttt ccatgtaatg gattgtaatg aattgcattg taatgggttt atgatgcgtt 5940 atgttggtaa gtagagggtt tttcatcctt cttatctgtt gtctatgctg tccatgcttt 6000 gtttactttt ttttttattt cttttcgcaa aaagtatgaa agcacagaga gaaagaaaag 6060 aaacgaaaac aatagaggaa aagaaacgaa attactttaa aaagcgaaat agaaaaaacg 6120 gaataacaaa aaccaaatag tttggaaaga aatactaaaa caagaaaata aaaactattg 6180 aaatgaaaag acaaaaaaaa aaacaggatt caatgctacc ataagcaaaa caaaagcatc 6240 ttgacaacaa atgtccaacg aatcaatttt tttttttggt ttattatttg tttctagtct 6300 ataatcaatc aaccttcaaa aagcccatcc attctttcat tcctggttca tttttttctt 6360 cttttcctcc catttttctt tcttctcctc gttattacgg ttttcctctc ttctcttctc 6420 tcctactaaa tcctctttat aacgatcctt tcctatccac taccatttct ccttcctctc 6480 tccattgctg gtttttttac cacactcctt tgcactacct tccacctcca tatcttctcg 6540 gttcctggcc tcccctcacc aatcgttgct cttctcacct gccgctcgca ttcatccatc 6600 tgcccttcac gtcctctcat tcgttcgttt cgcctatcct cac 6643 //