ID SPBC317 standard; DNA; FUN; 6435 BP. XX AC AL391174; XX SV AL391174.1 XX DT 08-AUG-2000 (Rel. 64, Created) DT 08-AUG-2000 (Rel. 64, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c317. XX KW SRF-type transcription factor. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6435 RA Wood V., Rajandream M.A., Barrell B.G., Collins M., Harris D.; RT ; RL Submitted (08-AUG-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9HGP0; Q9HGP0. DR SPTREMBL; Q9HGP0; Q9HGP0. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c317 is overlapped at the 5' end by cosmid c16D10, EMBL CC entry SPBC16D10, accession number AL035637, and at the 3' end CC by p1 p8B7, EMBL entry SPBP8B7, accession number AL032684. XX FH Key Location/Qualifiers FH FT source 1..6435 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c317" FT /map="IIR" FT misc_feature 1..186 FT /note="nominal overlap with SPBC16D10 S. pombe chromosome FT 2" FT misc_feature 405..581 FT /note="Match to PF00319 SRF-TF, SRF-type transcription FT factor (DNA-binding and dimerisation domain) Score 99.12" FT CDS 405..1523 FT /db_xref="GOA:Q9HGP0" FT /db_xref="SPTREMBL:Q9HGP0" FT /label=SPBC317.01 FT /note="SPBC317.01, len:373, SIMILARITY:Drosophila FT melanogaster, Q24394, mef2 protein., (516 aa), fasta FT scores: opt: 296, E():2.2e-07, (28.4% identity in 299 aa)" FT /gene="SPBC317.01" FT /product="SRF-type transcription factor" FT /protein_id="CAC01946.1" FT /translation="MGRKKISIAPITDDRSRSVTFVKRKQGLYKKAYELAVLADCEVAV FT TVIDRKGRLHVFCSSDYQRTLQQLNTLSIYELKNRSHFSSSPVEESSTVSPETTTGSFT FT PLNNKHLKSQDQPLSDSQLDTGDSPATSETTVQDYNPQVQSYCRPEPLSSNHVRSCPPF FT PPTQHHHPHTRPPHHPPHPHFHNNNYPPPYCFQSPVSPGATVPLQHHSPYPSDNGFPGH FT RRQTHFAPYYYPQRATSPSLKQVPTYLGTHVLQQDQSTYDQKVLMPPASYLSSPNQYTL FT KNVSPGNPACPPFLYEHPNPQLTPEMFDVKQGSPIPPTAYSGSSCETSQHTIANTPFLA FT YDRSPSLTNQEAESSFQDVASISPHSLSDVKY" FT misc_feature 1524..6435 FT /note="putative gene free region" FT misc_feature 6295..6435 FT /note="nominal overlap with SPBP8B7 S. pombe chromosome 2" XX SQ Sequence 6435 BP; 1963 A; 1315 C; 1115 G; 2042 T; 0 other; aaaaaaattt tttctattaa tatctttagt ttcgtcgccc gcttcaaccc agagtacaaa 60 aacaaaaata gcttcctcgt ttttaaatct caacttagat tcgtctaatc gactatcaat 120 tacgagcaca attggagaac tttgcgctct tgttttctca agcgaaaaat ctaccctcaa 180 gagatccggt gcagagaagg gaagacaggg ataaaaactg ttacataaat tccaagaata 240 aaccccatct gctgttttct atttgttcct tatatacttg cccgtttgct cgctcgtttg 300 ctcgtttgtt ttcgtcattc tccctatacc tgaattttgg ttaacctcgt cgactctttt 360 tctcttttct cgtcaaacct tactaaatat atacaacgtc taatatgggg cgcaaaaaaa 420 tctccattgc acccataacc gacgatcgtt caaggagcgt aacttttgtc aaacgaaaac 480 aaggtctcta caaaaaggcg tatgaactcg ctgtgctagc cgattgtgaa gtcgccgtca 540 cagtcattga tcggaaagga cgtttgcacg tcttttgttc ctctgactat caacgtactc 600 ttcaacaact caacactctc agcatatatg agttaaaaaa ccgttctcat ttttcgtcat 660 cgcctgtcga agagtcaagt accgtgtctc cagaaacgac aactggttca tttactcctc 720 ttaataataa gcatttgaag tcacaggacc aacctttatc agactcccaa ctcgacactg 780 gtgattctcc tgcaacatcg gaaacgactg ttcaggacta taatcctcaa gttcagtctt 840 actgtcgtcc cgagccttta tcgtctaatc acgttcgttc ttgtcctcca tttcctccca 900 cacaacatca tcatccccat actcgtcctc ctcatcatcc acctcatcct cattttcata 960 acaataacta tccaccgccc tattgtttcc aatctcctgt gtctccgggt gctacagttc 1020 cattgcaaca tcattctccg taccctagtg ataacggctt tcctggtcat cgtaggcaaa 1080 cgcacttcgc tccttactac tatccacaaa gagctacgtc tccttctttg aaacaagttc 1140 caacttatct gggtactcat gtactgcaac aagaccagtc cacctatgat cagaaggtcc 1200 ttatgcctcc agcaagttat ctttcttcac ctaatcaata caccttaaaa aatgtctctc 1260 ctggtaaccc ggcttgccct ccttttctct atgaacatcc aaatccacag ctaacgcctg 1320 agatgtttga tgttaaacaa ggctcaccta ttccgcctac tgcgtattcg ggtagcagtt 1380 gtgagacttc tcaacatacc attgcgaata ctccattttt ggcatatgat cgctctcctt 1440 ccttaacgaa tcaagaagct gaatcatcct tccaagacgt agcttccatt agtccacatt 1500 cactcagtga tgtcaaatac taaagaatat catagaagtc aatctccctt tcaaatgtct 1560 tcatcatgtg tcctccctgg atctaatgaa tcgtatacaa gtaataattt tagttggcat 1620 acatctcgat caaatcctta caatcgtttt cttactcaaa ctgcatatga tttggagcaa 1680 tccaagcacc aatagtttac tgccaagctg tgcgatgccc atttttttaa tttgacttac 1740 atcgcattcc agtaaacaat tgttaatact aggtctatca atttatcgtt atagttgaat 1800 tacgcgaacg aaattactga cctagcaata attgaagttt ggaatgttgt tcaataaaat 1860 ggacacctac cgatagctta tggattcaat gtgaactaca aacctagcat gatttcagac 1920 gctagcaaat acaaatacct gaacatttca gatgtgccaa tggtttaact ttgttaactt 1980 ccatcttcgg tattttggat cctccctgtt atgaattcaa gatggtgatt ttcggtttaa 2040 atatttattt gcaaactgcg ggcagtttat cacttattta ttcaggcacg cattttgtct 2100 attcaagtgg actaagtttt ttcgtgattt tgttttaacc acctccttcc acttcattaa 2160 ttaattcacc aacatgtaac attcgcggac caacctttat gttcgcattc aaattttttt 2220 ttggagccgc taatttctaa ttagacgttt ctcattcgtt ttcaaggtta tacatggttt 2280 attttcgtgt gtttgactcg attttttgct agatttttca cggtttctcc tttttaatca 2340 agaacgtttg acgattcaca gaaccttttt caaaccccta attacagttt tcaacaataa 2400 catgagaaat tcgaactttc cgtgtagttt tacagagtaa tgtttaagtg tttgttagta 2460 tgtcggtgtg taagtgtagc gtagcgataa ccgtccagct tcaaaggtaa gatgcagggg 2520 agtaatgcag cagctaattt tatatggcgg aatgagcatg gtagcactct tcaaagaagg 2580 tggaatagga atttggcttc atgcgaaatt atgttaacag aaacctgagt ttctgtaaca 2640 tattattata tacgtggctt aggacatcca aacaaataaa tgaaattctt tagtataaga 2700 agatgaagaa gatataaaag attaccaatg ttatgcaaac gatggaacat gaatcaaccg 2760 cataaaactc agtcgctgta tcaaaaatga ccatcgtggg atgctgacat aggcaatcaa 2820 gtttctcact tcaaataaat tagagactga tttggcaaat atttgagaat tgatcctcta 2880 tttgacaaat cattttaact tattttatga cagacaagtc tgtttgtatt ttgggaataa 2940 aacctcgcat tatccaatta aaacgtctgt tgcgaccacc ggcttaactt agaacaatta 3000 taaagacttc cagaacaaac aaaagtaata aattgtcgta aagcgacaat tatgcaaact 3060 ttttaccctg tgggataatt accaaaaaga tgatcacaag taacgtaagg ctatgtcttt 3120 caggtacaaa acaactgacg aaaggtgata aaagtattaa gtagaggcat ctggatgctg 3180 ggaaaacaaa ttggaaattt cgtagggcag tttaacatta tttaatggga acgaaaatag 3240 aaaataaaat attgcaaaaa gaatggcgtg ggtcttacta aaagacgcaa attagggata 3300 taaggcaatt aaactgcaaa tttaatgcga ataaaaacaa tagacaacgg cttagcaagt 3360 gaatttacaa tactcggatt tagattttgc gcgaaaaagt aaatgcattg gtcaacagga 3420 atgcaaccaa tgcaaaaaga attgtcgcaa ccttgttaaa aaaacagaaa tgtttgttag 3480 acagttacaa agatagcgat aatcgagacc aaagtgttcc taaaaattcc gatcggaaag 3540 tcagtaaatc tcgcataagg tggtgcaact ttgctgcata caagaaacta gcagcccctg 3600 gccatttcag ctttttcaat ttgcttagag cacaacacta agcaaagaat ctctttcttt 3660 tataatagca caactcccaa atgattatca ctttttctaa aacaaattaa ttggatactt 3720 ctaaagtatc caattgtaat aagaagtctc agaataatcc aacaaatcat ttcaaatatc 3780 aggacgcaga agcgagcatg tagagaatat tattccatct caatactcgt ttaaaaggca 3840 agcgattcaa aagaatggta agggtagtgg atgtttgcat gctgatttgg aagggctgcg 3900 gatatccatg agaataagca tcgtctaatc tttataggta tagcaggaaa aagatgaact 3960 tcttatgaac ggttagcaca gagaacgagg gagcgaattt ttgatttaag tgatacaatg 4020 caagtagtct ataaatttgg tcctttttta gaactccgat tgtctcaaag agtgtaaata 4080 tacacgcgaa aactgaaaag gtccgactaa attttcgcag ccttctcggc ttcttttaac 4140 acatggaaag caattttttg ctttttattg ttcaacaaaa cctttttgat cattatatca 4200 tttcaattgt ctcaaataga attataggaa tcagagcaag cactagaaaa gtcgagtacc 4260 aattttttct agctttcaag agctttccct ccatcatggg tcgaacaagt ccaaaatttc 4320 atataatcaa taaacacttt ttgagccttt ttaagcggga aaaatgacag tcttaacgat 4380 cacaaaaaca agcaaaaatt gcaaaagtgt ttgggataca tttttataga tttcatggtt 4440 tcctcgctaa tatggtgaaa aaaaatttta tgcttttccc tacgtcaatc caataaagac 4500 aattaaacac cagaatagca ctggtgtatt ttttctggaa tgctgtattt gagacatacg 4560 aagaggcttg attaatgtaa tggtcaacga acaagtaagt gtcttttctc tcctcgtgag 4620 tgtagtgacc gttgaggaat ctaatggtga ttcaaacttt ccaaacgaag aaagttttta 4680 acttatacgc tactacacac aagcattact ctcatgccgt tccccccaaa cttataggtg 4740 tctcacgact ttgaacaaag caggcaagtc acttgcttat aggaatacgc tcgtataaat 4800 aaaagaatct cgttttccga ccccaattat ccccttccca attgtggtgt cgaggccttt 4860 ttatgcacat gatcttaata aagatttagg ggatgtgggg tgttatttcg caacttggtc 4920 aatgtttttc tagtgtacct ctgttagcga ggttatgtag cccagcgttt gttatctagt 4980 agcacatttg acgttgaggt cgttgtaatg atgaaacagg acacgaacat gtaccaccga 5040 catgcatagc tctttatttg gctgcgacat gggttaccta ggtggggagt ggtactgttg 5100 gaactttata tgaattgcaa acagaaattt ttcgttgcgt tgattcagtt tgagacatta 5160 caaattatgt gattcgttgg aaatgtgtag atccgaatta tgtattaatg tggcttgctt 5220 tttttcctta ttggtttgat cctgagaaag tgctgagatg gaatgactga accttgctag 5280 taaaaactag ttttccggga tcggcaaaca tgttttgcaa ttgaaaacaa aatctttagc 5340 tgtgttagta atttgcaatt agcaacgcgc aaatcctgcg atgaggatag tatgtgagtt 5400 gctaattgca acatctgaag cattctgatg tgtcccattt ttccaaaccc ggatacaggc 5460 ggcattgaga aacaccacaa acaaaaaatt tccagcagtc gcaatgtaaa caatgacagg 5520 gttagccaca tatctggcta taatggttaa ctagcttgtt tgatgatgaa actagttgaa 5580 aattaaaaac tgcgaaaaaa aattccttac cgggttaact caattactcc catacacctt 5640 ttcccaccaa cttaacaaaa aacccatatc tctcaatatg tccgctccgt gagaagaagt 5700 aatacttttt tatgcattcg gctaaatgca accctgcctt ttgaacatcc caattggcga 5760 gttctcttca atgttaacca acatatcatg gcgtccacga cagcacatta cggcaaaatt 5820 ctaccactga tttcacactt tcacctactg aatttttcat accaagtaag cctacaaact 5880 agactattta tcttatagga aatgtatttt ccctgatact cttatcaatc aacgcctact 5940 ggtttgtttg agcgtttttt cgttcaataa ttcaacactt tcactactaa caattcacct 6000 atgttacagc tttgactcct tactttccct gtctttcggc tgttccactt tgaccatcgt 6060 aagcctattt tgtcattact gaagatgtca ctacacgtta cattaactaa tgcaaacgat 6120 gttccgtgct gcttttttta tttctagctc tactgtgttg gtattaagtc ttagtctgtt 6180 tatttcatag tggtctcaac aatgaatctg gtgtattcga tttataaatt tgtggttcga 6240 cattgagtac ggatgaccct gtatgaaata acagtctcct gaatcgctta tgatgatcct 6300 tatcgtgttg ttcgaccttt ccaattccgg ggttgttacc ttatgtccac ctattagtat 6360 gatttgcgcc agttcatatg atggaatttg cattggagac aggttgaacg atttttcagg 6420 ttattttgcg gactg 6435 //