ID SPBC25D12 standard; DNA; FUN; 15926 BP. XX AC AL031158; XX SV AL031158.1 XX DT 31-JUL-1998 (Rel. 56, Created) DT 02-FEB-2000 (Rel. 62, Last updated, Version 3) XX DE S.pombe chromosome II cosmid c25D12. XX KW cdc47; cell division control protein 47; LTR; MCM family; mcm7; KW minichromosome maintenance; N2,N2-dimethylguanosine tRNA methyltransferase; KW pescadillo-like; ribonucleoside-diphosphate reductase small chain; KW rna helicase; serine-rich; splicing factor; SR splicing proteins; suc22. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-15926 RA Lyne M., Rajandream M.A., Barrell B.G., Whithead S., Chillingworth T., RA Churcher C.M.; RT ; RL Submitted (28-JUL-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O60164; O60164. DR GOA; O74356; O74356. DR GOA; O74357; O74357. DR GOA; O75001; O75001. DR GOA; P36603; P36603. DR GOA; Q9P804; Q9P804. DR SPTREMBL; O74354; O74354. DR SPTREMBL; O74356; O74356. DR SPTREMBL; O74357; O74357. DR SWISS-PROT; O60164; PESC_SCHPO. DR SWISS-PROT; O75001; MCM7_SCHPO. DR SWISS-PROT; P36603; RIR2_SCHPO. DR SWISS-PROT; Q9P804; TRM1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c25D12 is overlapped at its 5' end by cosmid SPBC19F5, CC EM:AL022599 and at its 3' end by cosmid cosmid SPBC13G1, EM:AL022600. XX FH Key Location/Qualifiers FH FT source 1..15926 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c25D12" FT /map="IIR" FT CDS join(complement(1555..1576),complement(1349..1516), FT complement(1..1194)) FT /db_xref="GOA:O60164" FT /db_xref="SWISS-PROT:O60164" FT /label=SPBC25D12.01c FT /note="SPBC25D12.01c, len:460, SIMILARITY:Saccharomyces FT cerevisiae, YG2S_YEAST, hypothetical 69.9 kd protein in FT mic1-srb5 intergenic region., (605 aa), fasta scores: opt: FT 1286, E():0, (50.2% identity in 464 aa)" FT /partial FT /gene="SPBC25D12.01c" FT /gene="SPBC19F5.05c" FT /product="pescadillo-like hypothetical protein" FT /protein_id="CAA20097.2" FT /translation="MARVKQKGKAGAARIYITRNQALKKLQLTLADFRRICILKGVYPR FT EPKNKKKANKGSTAPVTFYYTKDIQYLLHEPIVQKFREYKVFARKLSKALGKGELETAK FT RLEARKPTYSLDHIIKERYPTFHDALKDIDDALSMLFLFSTMPVTDKIGAATVANCERL FT CAEFQHYVIRSNSLRKAFLSIKGIYYQAEIFGEQITWIVPYKFAQSVPTDVDFRIMHTF FT LEFYQALMGFVNFKLYNTLGLRYPPKIDVAKSESAAGLAAYELEESSSLPAIVHGNNKN FT ARKNIATLKSKIRDIVNSDANVVEQSEKTTEDADEEPETEENLDEFKPADGADNEDSKS FT LVSHISSSNTSLFSNFTFFLSREVPRFSLEFVIRAFGGKVGWDPILGSGSPFSESDPVI FT THHICDRPHISQKYEGRIYIQPQWVYDSINKGILERTDLYACGATLPPHLSPFVKVGEN FT DYD" FT misc_feature 1..1779 FT /note="nominal overlap with cosmid SPBC19F5, EM:AL022599 FT S.pombe chromosome 2" FT misc_feature complement(62..334) FT /note="Match to PF00533 BRCT, BRCA1 C Terminus (BRCT) FT domain Score 29.92" FT misc_feature complement(1195..1210) FT /note="ctaataaatataatag, splice branch and acceptor" FT misc_feature complement(1343..1348) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(1517..1530) FT /note="ctaattgaaaacag, splice branch and acceptor" FT misc_feature complement(1549..1554) FT /note="gtatgt, splice donor sequence" FT LTR complement(1990..2339) FT /note="lone LTR - tf1 type" FT CDS join(complement(4866..5008),complement(3157..4813)) FT /db_xref="SPTREMBL:O74354" FT /label=SPBC25D12.02c FT /note="SPBC25D12.02c, len:599, SIMILARITY:Mus musculus, FT O70567, dentin sialophosphoprotein precursor., (932 aa), FT fasta scores: opt: 354, E():1.1e-11, (24.5% identity in 478 FT aa)" FT /gene="SPBC25D12.02c" FT /product="hypothetical serine-rich protein" FT /protein_id="CAA20098.1" FT /translation="MRTTLRLHIIKDGEQDNQFMILFDPSSSISLLKEKVQETYKSLYP FT FESNINIRNIKNEESYDIPNEYLVGEIFPTNSKVIVESFSSPLKKLDGTMINFKEKNIQ FT HDLDGVENDFATVQSASNGVHAINGKRTHPDESENPRKLPKKNFVEAIDANSPGFVYRP FT TSIRDRAYSISSNHDNESTLTEGIALKEIESPDKDRKADGIVNLSVTQEEDDNHQSFNS FT SLTPSQPTTYNRANFFSINDASSDSSSDAPLRTLSSPSRLRMKDNDRKYLVEHSPAALI FT KESETIDGIDDKSLRSSTREVSVESPNEDSVNDDSSSDVSDEKETEAKHEIRAPAIIVR FT ETSSHPSTAVPSENDTTESENDTLSESSTTSISSSPSENSDTSDDLTKVDSPNKSLVND FT NVSAKHDKESENGKSKFPPPSQTLVTTSTISAAGNEPSDEIGSENDSDSDSDSDSSVPL FT SQLQKKSQQRNSVSHEIQNRGTKGSPKEPKAKPSTERPETHRTLSYSRLSELSKTFSPE FT IREPSLTKKTAVSMQESKEEGRSDESSESEESGSSSDESDNSEKEDRSNPIPVEKRAST FT VLNTKKKRKAKRNSALAGLAALV" FT misc_feature complement(4814..4832) FT /note="ttaacgaaaattattatag, splice branch and acceptor" FT misc_feature complement(4860..4865) FT /note="gtaagc, splice donor sequence" FT CDS complement(5595..7877) FT /db_xref="GOA:O75001" FT /db_xref="SWISS-PROT:O75001" FT /label=mcm7 FT /note="SPBC25D12.03c, len:760" FT /gene="SPBC25D12.03c" FT /gene="mcm7" FT /product="minichromosome maintenance protein mcm7p" FT /protein_id="CAA20099.1" FT /translation="MALPTIDLKIPEYEECQHKITDFLSHFKQEQVQDGQQNQDISMSD FT AGDEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSDTQLLHNIESNAKRFVELFS FT QCADALMPPPTVEINYRNEVLDVIMQQRVQRNENIDPEHKGFPPELTRGYDLYFRPVTR FT NKKPFSVRDLRGENLGSLLTVRGIVTRTSDVKPSLTVNAYTCDRCGYEVFQEIRQKTFL FT PMSECPSDECKKNDAKGQLFMSTRASKFLPFQEVKIQELTNQVPIGHIPRSLTVHLYGA FT ITRSVNPGDIVDISGIFLPTPYTGFRAMRAGLLTDTYLECHYVSQIIKNYTNIEKTPQS FT EAAIAELNQGGNVYEKLAKSIAPEIYGHEDVKKALLLLLVGGVTKELGDGMRIRGDINI FT CLTGDPGVAKSQLLKYISKVAPRGVYTTGRGSSGVGLTAAVMRDPVTDEMVLEGGALVL FT ADNGICCIDEFDKMDESDRTAIHEVMEQQTISISKAGITTTLNARTSILAAANPLYGRY FT NPKVAPIHNINLPAALLSRFDILFLILDTPSRETDEHLAQHVTYVHMHNEQPKMDFEPL FT DPNMIRHYISSARQYRPVVPKDVCDYVTGAYVQLRQNQKRDEANERQFAHTTPRTLLAI FT LRMGQALARLRFSNRVEIGDVDEALRLMSVSKSSLYDDLDPSSHDTTITSKIYKIIRDM FT LNSIPDVEGNERSLTLRAIRERVLAKGFTEDHLINTIQEYTDLGVLLTTNNGQTIMFLD FT PDLHMEN" FT misc_feature complement(5877..7382) FT /note="Match to PF00493 MCM, MCM2/3/5 family Score 946.36" FT CDS 9712..10887 FT /db_xref="GOA:P36603" FT /db_xref="SWISS-PROT:P36603" FT /label=suc22 FT /note="SPBC25D12.04, len:391" FT /gene="suc22" FT /gene="SPBC25D12.04" FT /product="ribonucleoside-diphosphate reductase small chain" FT /protein_id="CAA20100.1" FT /translation="MGLEHLEEFSYPKEHGEEVEYDSEQGVRKIYVKSIKETFNFDNVS FT EEEKQEGGDYYLGKKEDELDEVVLRPNPHRFVLFPIKYHEIWQFYKKAEASFWTAEEID FT LSKDLVDWDNKLNADERYFISTVLAYFAASDGIVNENLLERFSSEVQIPEARCVYGFQI FT MIENIHSETYSLLLDTYIREPKEKQRHFDAILTMGSIKAKAKWALRWINDEDSTYAIRL FT VAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELICRDEGLHTDFACLMFSHLKHRP FT GRKVVEAIIVEAVDIEKEYFTDALPVSLLGMNKDLMCQYIEFVADRLLVALGNDKYYNV FT TNPFDFMENISLAGKTNFFEKKVSDYQIAGVMSGTKRAEKDDHTFTIDEDF" FT misc_feature 10003..10809 FT /note="Pfam match to entry ribonuc_red PF00268, Ribonucleot FT ide reductases" FT CDS 11340..12986 FT /db_xref="GOA:Q9P804" FT /db_xref="SWISS-PROT:Q9P804" FT /label=trm1 FT /note="SPBC25D12.05, len:548" FT /gene="SPBC25D12.05" FT /product="N2,N2-dimethylguanosine tRNA methyltransferase" FT /gene="trm1" FT /protein_id="CAA20101.2" FT /translation="MYEANMLRLSQRISNAVNHFSTAMMSGASASLTEGSAIIPFSNPN FT EVFYNPVQQFNRDLSVTAIRAWSETRSKKIVAKSHHRHQLLDQNSELSQENLTKCDTTH FT DFEKNCSEKTDSTSADNIAGFTILEALSATGLRSIRYAKELPNVKRILANDLLENAVKT FT IEKNVNYNNVSDIVIPNKGDANAVMHMNKFHYDVIDLDPYGSAAPFLDAAVQSVSKDGL FT LCITCTDSAVLAGNAYPEKCFSNYGGSSLRSNFCHEQAVRHLLYAIAASAAKYGRAIKP FT LLSLSIDFYFRVFVQIKAKPVLVKNLQSQSLLIYHCSGCGSFAEQPLGKTSPGRLPGTT FT KFSNASGPPVSANCEHCGYVHHVGGPLWGGPLHDAEFLKKMRAIAEDLDPEVYGTKRRI FT LGMLALADEELPDVPFYFVLSQICSVLRSQSPPQNIFVSALLNAGYRVSGSHAKSNAIK FT TNAPWSFVWDVLRSWIKDHPVKLENISKTSAGAAILEKTPTAEVDFTFRPDSEFASKKE FT GYTRYQMNPTENWGPKSKPGKRTIAEVDSKS" FT misc_feature 11397..12767 FT /note="Match to PF00268 TRM, Ribonucleotide reductases FT Score 977.88" FT misc_feature 12309..12332 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 13387..15084 FT /db_xref="GOA:O74356" FT /db_xref="SPTREMBL:O74356" FT /label=SPBC25D12.06 FT /note="SPBC25D12.06, len:565, LOW SIMILARITY:Arabidopsis FT thaliana, Q9ZNU1, rna helicase, (505 aa), fasta scores: FT opt: 141, E():0.088, (20.2% identity in 456 aa)" FT /gene="SPBC25D12.06" FT /product="putative rna helicase" FT /protein_id="CAA20102.1" FT /translation="MGLRLNGRSVPWLLQTRKFINITSPRYTAGRPVILNENQLIRYLP FT NYTNKGDEEVTRRKKLHEPNGPKFHELPLNQNILDGLSTNFAEYKNSTPLQQRIINALM FT KSGVSFIVRGWNGSGKSFGAMLSTLSMYFEALKFLKHNPVAGKPVQSGCQVLFIVPNDN FT LAAQYQFWIERLLHGITEKEELQHIYKILTLTPASLDSFQNRPPYIGITTFPNLQHCIK FT KNQPILKQFTSQLQLLIVDESDLVIPDHGRSRRLSSPEILADKIFLSSFIQLCKKNLRI FT NMDDENVGGMGIHSQLHGKGSIPTMVFMSASNSKNGVNYLSRYITDQLGIIGIDRKHQW FT YWNLTSTPSVFHYLIKAQAEPKTTKVTLTNLPYEFVRFNNSMHSIGDNNFSYNNSEAVL FT QIFAQSVPRILNIEAKKSKLENNILKCVMIVLPKEFNPHSFCNQYKGFAVGNRFWQCRT FT LSTESLNFSTNLNNVVFVANPSEIRGLHLPSITHIFHLWSTFSGVAYQHIAGRLGAMGQ FT TGKIFNFIIPDYYKSNDFKKTDFRRCILFMLQRVGIRPKSYEFDDEHF" FT misc_feature 13726..13749 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS join(complement(15834..15926),complement(15662..15767), FT complement(15289..15614)) FT /codon_start=1 FT /db_xref="GOA:O74357" FT /db_xref="SPTREMBL:O74357" FT /label=SPBC25D12.07c FT /note="SPBC25D12.07c, len:174, SIMILARITY:Homo sapiens, FT Q15287, rna-binding protein., (305 aa), fasta scores: opt: FT 245, E():1e-09, (32.1% identity in 159 aa)" FT /partial FT /gene="SPBC25D12.07c" FT /gene="SPBC13G1.14c" FT /product="similar to SR splicing proteins" FT /protein_id="CAA20103.1" FT /translation="FSHRSSSRDSFSSNRSYSSSLSRSPEEPLRTILVENLTRNVTKEH FT IAEIFGIYGLIDHIFMPIYKKSELNKGYCYIEYVYHDQAANAVDKMNNAELDGEELFVS FT IKRFPFESLHKNHKHYENSYRPSRSQNNSHYNDKSFHRSRYSRARSRSPGSNISEYSDQ FT SPPYHSYRHRP" FT misc_feature join(complement(15662..15767),complement(15505..15614)) FT /note="Match to PF00076 rrm, RNA recognition motif. (a.k.a. FT RRM, RBD, or RNP domain) Score 51.99" FT misc_feature complement(15615..15626) FT /note="ctaactttgcag, splice branch and acceptor" FT intron complement(15615..15661) FT /note="confirmed intron" FT misc_feature complement(15656..15661) FT /note="gtgagt, splice donor sequence" FT misc_feature complement(15768..15786) FT /note="ctaatacgatgcaaaatag, splice branch and acceptor" FT intron complement(15768..15833) FT /note="confirmed intron" FT misc_feature complement(15828..15833) FT /note="gtacgt, splice donor sequence" FT misc_feature 15856..15926 FT /note="nominal overlap with cosmid SPBC13G1, EM:AL022600 FT S.pombe chromosome 2" XX SQ Sequence 15926 BP; 4978 A; 2783 C; 2842 G; 5323 T; 0 other; gatcgtagtc attttcacct actttaacaa atggactaag atgaggaggg agcgtagcgc 60 cacaggcata caagtctgtc ctttccaaga ttcctttgtt gatagagtcg tatacccatt 120 gtggttgaat atatatccta ccttcgtact tctgtgaaat atgaggacga tcacatatat 180 gatgagtaat cacaggatca gattctgaaa atggagaacc tgatccgaga attggatccc 240 atccaacttt accaccaaag gcacgaatca caaattccag agaaaaacgg gggacttcac 300 gagaaaggaa aaaagtaaaa ttagaaaaca acgaggtatt agatgatgaa atatgtgata 360 ctaggctttt ggaatcctca ttatctgcac catcagctgg tttaaactca tctaagtttt 420 cctccgtttc aggttcctca tcagcatcct cagtagtttt ttccgactgc tcaacgacgt 480 tcgcatcact attcacaata tctcttattt tactttttaa agtagcaata ttcttacgag 540 catttttgtt attaccatgg acgatagcgg gtaaggaaga agactcctct aactcataag 600 ctgctaaacc agcggcagat tcgcttttag caacatcaat cttaggggga taacgaagtc 660 ccaaggtatt atataacttg aaatttacaa accccataag tgcttgataa aattccaaaa 720 aagtgtgcat gatacgaaaa tcaacatcgg taggaacaga ttgagcaaat ttgtagggaa 780 caatccaggt gatttgctca ccaaaaatct cggcctgata ataaattcct ttaatagaga 840 ggaaagcttt acgaagggaa tttgaccgaa ttacatagtg ttgaaattca gcacataatc 900 tttcacaatt agcgacagta gcggcaccaa tcttgtcggt aacaggcata gttgaaaata 960 agaacagcat actaagagca tcatcaatat ctttcaaggc atcatggaag gttgggtaac 1020 gctctttaat gatatgatcc aaagaataag tgggttttct ggcctctaaa cgtttagcag 1080 tttctaattc cccttttccc aatgctttgc taagcttgcg agcgaacact ttgtactcgc 1140 gaaacttctg tacaataggc tcatgtaaca aatactggat atccttagta taatctatta 1200 tatttattag tacataattt cattctttta gttcatcaaa aaaagactag cccatgataa 1260 aattgaccat tcggaaaatg ctttttatac caaacgtcca tgttcagata gctatgattc 1320 cggtaaaaat tgatgctatt agacttacag aaagtgactg gagcagtgga gcctttattt 1380 gcttttttct tattttttgg ctcacgagga taaacaccct ttaaaataca aatacgacga 1440 aaatcagcaa gagtgagctg aagcttctta agcgcttgat ttctcgtaat gtagatacga 1500 gctgcacctg ctttccctgt tttcaattag aaaatttttt tacaagctac atacctttct 1560 gttttaccct cgccatttct tcaatttgat ggctttttgc agaaagaggg tgttggaaga 1620 attttgcaaa atccaaaatc cttcatacag actagcttag ctctctgcac tgcgtaaatg 1680 gtgttagatg gtagtattat ctcacagcgc taaattttct tatactattt tacgttaaat 1740 ttcgctatat ttgtgttttt ccgtactttt gagataaatc tttgcggcaa gggactaatt 1800 gatttttgat aagtttgtaa aggtcttgta atttaaaata actatcaaaa cttctacaat 1860 tactccaagt gaatcaatgt tcgacgattt agttgttagc tactcaagta agttataatc 1920 ttaagcaata cagtagtgat atattaaagt atttatgttt attacatttt acggtaaata 1980 aacaaatctt caggtacgca gctaggcatc tgttttaacg acaggtagag ttgcgatgag 2040 tcttccttgt gatttattaa attatatagt acgcatatga tatatatagc aatttgtaat 2100 agatattctt atattcaggt tacgtcgttt tctgtcacta aaatattatt atatgagcct 2160 cgctcctcag ttcagttgta gcttccattt atcattttta taataattta tatcaacggt 2220 ttttgtgtta aatagataga cgggtatacg cagttattaa taatctgatt tcacttcaac 2280 ttatggcata caaagttact caacgttgaa taacatagca tagtgtatca gcgctgagat 2340 ttatgagacc gtatgtatat gtttgtgtaa agggaaaatt cacccaattt atcgtattgc 2400 taaagcactt tatagtaaga gtgtgataag tttcttttcg atcatcttac ttttatagag 2460 ctattatttt actacaaata aataagttcg ttcactttgc ataatatcaa ctatacgcag 2520 ttagtttgcc cggatcaaga aattcatgtt tagctggtct tcgttataaa catactaaag 2580 aatagaaaga ttttactaat atcagttttt gttaacacta ttgaaccatt atgagtaatg 2640 tcgagggtaa agtaattact atacaacaaa taatggcatt ttttgtgaat ttaggtactg 2700 cctataataa tataaatact aaagctcgtg aaaatatttg aatattgtaa tctaatttgt 2760 tctttttttt tacgaatttt agtccggaaa attttactaa tatactaatt actgtcattg 2820 tctggtctcc tgtttcctac aactagcagt tttaatttta caatcagtca tcaaaaaagg 2880 atttgaaatg aagcaactta atggacatta aagaacgttc catttagaat ttcaacatct 2940 ttttatcagc atcaaagtta aatcaagttt tgctagctaa ttaacatgta attttattaa 3000 taagtcaaaa ttatgcagaa agccaagaaa actaaattta ttatatacgc aatatgatag 3060 cacaacaacg ctattacaga attcttttgc tattggactt atgatgaaaa aaaaaaaaaa 3120 aacgagcgta aagggagtta atcaaaaaca agaaatttac actagggccg ccaagccagc 3180 caaagcactg tttcttttgg ctttcctctt tttctttgta tttagtacag tacttgctct 3240 tttctcaacg ggaataggat tggatctatc ttccttctcc gaattgtcgc tttcatctga 3300 agaactccct gactcttcac tttcagaaga ttcatcagaa cgcccttctt cttttgattc 3360 ctgcatactg accgcggtct tcttcgtaag agaaggttct ctaatttcag gagaaaacgt 3420 tttagacaat tctgaaagac gtgagtaaga caatgtccta tgagtctccg gtctttccgt 3480 agaaggctta gctttaggtt ccttcgggga tcctttggtt cctctattct gtatctcgtg 3540 agatacagaa tttcgttgtt gtgatttctt ctgtagttga cttaaaggaa cagaggagtc 3600 cgagtctgaa tccgaatctg aatcattttc agaaccaatc tcatcagaag gttcatttcc 3660 cgctgcagat atcgtcgatg tagtaacaag ggtctgggaa ggagggggaa atttgctttt 3720 accattctca ctttctttat catgcttagc tgaaacgtta tcattaacca gagatttatt 3780 cgggctatca accttcgtta aatcatcact agtatctgaa ttttcggacg gggaagaaga 3840 tatagaggtc gtagaggatt cagaaagggt atcgttctcc gattcggtgg tatcgttttc 3900 ggaaggaacg gcagtggatg gatgagatga agtttctcta actattattg caggagctct 3960 aatttcatgc tttgcctccg tttctttttc atcggataca tctgatgaac tgtcatcatt 4020 gactgaatct tcatttgggc tttcaacaga aacttctcga gtagaagaac gcaatgattt 4080 atcatcaatt ccatcaatgg tttcactttc tttaattaat gcggcaggag aatgttcgac 4140 taaatatttt ctatcattat ctttcatgcg aagacgggaa ggcgatgata aagttcgcaa 4200 gggagcatct gacgatgaat cagaagacgc atcgttaatt gaaaaaaagt tagctcgatt 4260 ataagttgtt ggttgacttg gtgttaagga actattgaag gattggtgat tatcatcttc 4320 ctcctgagta actgataaat taactatacc gtcagccttt ctatctttat ctggactttc 4380 aatttctttt aaagcaatcc cttcggttaa tgtactttca ttatcatgat ttgaagaaat 4440 tgagtatgct cgatcgcgaa tactagttgg tctgtataca aatccaggag agttcgcatc 4500 aattgcctct acaaaatttt tttttggtag ttttcgaggg ttttcactct catccggatg 4560 cgtacgcttc ccattgattg catgaacacc attagatgca ctttggacag tagcgaaatc 4620 attttcaacg ccatctaaat catgttgaat atttttttct ttgaagttta tcatagtccc 4680 atctaatttc ttcaacggag aagagaatga ttcgacaatg accttggaat tagtgggaaa 4740 gatttcaccg actaagtact cattaggaat gtcgtagctt tcttcatttt ttatatttct 4800 tatgtttata tttctataat aattttcgtt aatagtagaa agatggagca tgctgctatg 4860 cttacgattc aaaaggataa agtgatttgt aagtttcctg cactttttct tttaataaag 4920 aaattgaaga agatgggtca aacagaatca taaattgatt atcttgttca ccatctttga 4980 ttatatgaag ccttaatgta gttctcattt cgaatattca gcaaatttct ttatttgatt 5040 cttacggaga agtggatccg gcaaactagt tataatggaa ttaatcagta ttaaaaagac 5100 gttaaaacaa agtaagttat ttaaaatgga atgcccattt cttgacacca atgggtatcc 5160 aaagttggtt gctctaagga agtgcgtaaa gttctattcg caatttactc ggcatgacag 5220 caggaagggt gggacagcag tgatgaacaa gttaaaggtg taaaataatg ctaaatgtca 5280 agaaataaaa actcattact cattcctacg actcgagcat attgacacgt atcttaaggt 5340 tttatggtcc ttccaaatct aacgaaacat attggcttaa tatatcttcc tttcaattgc 5400 gtgaattaac atttgaatac aagtaaataa actatcatag attaaaagaa cgacagatgt 5460 attagtcaga attaaagaat attcaacaga aaagtagttt gacgtctcag aataatttct 5520 ataggttgta aattagctta aagcaattaa ttggcagaaa aattaaatga taggagttat 5580 ctattagtaa attgttaatt ctccatatgt aaatccggat cgaggaacat tattgtttga 5640 ccattgtttg tagttaaaag aacaccaagg tctgtatatt cctgtattgt atttattaaa 5700 tgatcttcag taaaaccctt agcaagtacg cgttcacgaa tagcacgtag tgtaagtgag 5760 cgttcattgc cttcaacatc tggtatgcta ttaagcatgt ctcgaataat tttatatatt 5820 ttcgaggtaa tagtagtatc gtgagaacta ggatccaaat catcgtatag tgatgacttt 5880 gaaacagaca ttaatctcaa agcttcatca acatcgccaa tctccactct attactgaaa 5940 cgcaaacgag ctaatgcttg tcccattcgc aaaatagcga gaagtgtacg tggagtagtg 6000 tgagcgaatt gacgctcatt ggcttcatca cgcttttggt tttgtcgaag ttgaacataa 6060 gcaccagtaa catagtcgca tacatccttt ggtacgactg ggcgatattg tctagcagaa 6120 gaaatataat gcctaatcat gtttggatct aatggttcaa agtccatttt aggttgttcg 6180 ttatgcatgt gaacatatgt tacgtgttgt gccaaatgct cgtctgtttc acgagaagga 6240 gtatctaaaa ttaaaaataa aatatcaaac cgggaaagca gtgcagctgg aagatttata 6300 ttgtggattg gtgccacttt cggattatat cgtccatata aggggttcgc agcggccaaa 6360 atacttgttc gagcatttaa agtcgtagtg attcctgcct ttgaaattga aatagtttgc 6420 tgttccatta cttcatgaat tgctgtacgg tcactttcat ccatcttatc aaattcgtca 6480 atgcagcaaa taccgttgtc agcaagtact aaggcacctc cctctaaaac catttcatcc 6540 gtcactggat cacgcattac agcagcagtt aaaccaacac ctgaagatcc acgacctgtg 6600 gtgtatactc ctctcggggc aaccttcgaa atatacttta ataattgaga ttttgcgaca 6660 ccaggatcac cagttaaaca aatatttatg tcaccacgaa ttctcattcc atcccctaac 6720 tctttagtaa cacctccgac aagcaacaga agtaaagctt ttttaacatc ttcatgtcca 6780 tatatctcgg gagcaatgga cttggccagt ttttcgtaca cattgcctcc ttgatttaat 6840 tcagcgatag cagcctccga ttgaggagtt ttttcaatat ttgtgtaatt tttaatgatt 6900 tgtgaaacat aatggcattc taagtaagta tcggttaaga gcccggcgcg cattgctcga 6960 aaaccagtat acggagtcgg caaaaatata ccagaaatat caacgatatc gccaggattt 7020 acagagcgag tgatagcacc atataagtgg acggttaatg atctagggat atggccaata 7080 ggtacttggt tcgttaattc ttgtattttt acttcctgaa aaggaagaaa tttactggct 7140 cttgtgctca tgaaaagctg accttttgca tcattctttt tacattcatc cgaaggacat 7200 tcagacattg gaaggaatgt cttctgacgg atttcttgaa acacttcata cccacatcga 7260 tcacatgtat aagcatttac agttaagcta ggcttaacat cacttgtacg agtaactata 7320 ccacggacag tcaaaagaga tcccaagttt tcgcctctta aatcacgaac gctgaatggt 7380 tttttgttgc gagttactgg tcgaaagtat aagtcataac ctcgggttaa ttcaggagga 7440 aatcctttat gctcagggtc tatattttca ttcctttgaa cacgttgctg cataattaca 7500 tcaagtactt cgttgcgata attaatttcc acagtaggag gaggcatcaa cgcatctgcg 7560 cactgcgaaa aaagttcaac aaaccgttta gcattagatt caatattatg caaaagctga 7620 gtatcacttg gatcgaactc gtacaaatca ttcaaatcaa cattaataac atttgactct 7680 cgattggaga ttttttgtag tatatccata tactttgatt ttaaaaaagg ctcatcacct 7740 gcatccgaca tcgaaatatc ctgattttgt tgtccatctt gaacctgttc ctgcttgaag 7800 tgggatagaa aatccgtaat tttatgttga cattcttcat actctgggat ttttaaatct 7860 atggtgggaa gtgccatgct tttcaaacca aaaaagtatc ccaaaacttt tctgctgaat 7920 tagcaaaaaa tataaaggtt caaaaagaaa gcaagggtat taagtaaggg taagatacca 7980 agaaaggttc ttctttaggt tgttttagtt agttggtagt atgaatcaac gcgaactcgt 8040 aaggtaacaa aaatgtaaat attgtaactt agggcatata caaattgtca ttgactctaa 8100 agaaattgca gtgtataatt tttttgattc tctacatatt tcctttttaa tttattactc 8160 tcgcagcaaa agtaaacatt cagtttgatt ttaaaagcca ctatgtagta ctgttagaaa 8220 ttttttttgg agagtattca ctatgtataa ataaacagtt tatttgtttt aaattaataa 8280 taatcatttt gccagacaat aaattggtca ttttattttc ataaatgtat gtctaattgt 8340 tttcattgct tcaatcgatt gttattttag ctatccataa tttttcctat ttttcttcta 8400 cacgttttgt atttctaaac atgagagtta tttagactac tgcctaaaat ttatttcact 8460 tttattttta aaaatttttt tgaaaactta tgaagtaagt tagcagggaa attctcaaaa 8520 gaaatgcgat atcaacaata taaaaacttc gagtagggaa ctttttattt tttgttcggt 8580 tatttcaaac atcagtgaag aatgaatata gaaaattctt tattgtttta taccacattg 8640 taaactgtac ctaaaagcac aacacgcaat agaaacgtgc tattggaatg cttatttatt 8700 ttctgtttac aatagacgaa tataagtatt tctttttttt tttacgaaag gatcaaggta 8760 ttaaaattat tcacagtatg tggctaggtc tctgtcgcgc ttatcttttt ttaattaagc 8820 aatgtcttta tgtacggtcg cgtcgcatta gaagtttcgt gatttgtaat atttgagcag 8880 ttataaagcg ctctacatct cggatgaaaa ggagattgtg aagagatatt tatttctaac 8940 atcatatcgt actactaggc tgatatcatt gcgtttcatc tacaacataa attgaggtac 9000 cctgacgcgt cttaagtatt acactgaaaa ctactttatt aaattataac aaaaagtgag 9060 aaatttaata atttatttat tacataaagt aataaattca tttctatttt ttcaattatt 9120 tgagcctaaa taattgaatt tattcgactc cacgcttagc atcgaattct tcgatttaag 9180 ctatcacatt tttctattaa ctagctacaa tttcctgctt taagaaaccg ttattataac 9240 acaccagaag ggaaaggaag taagcccttg cttttcccat ttaatttgag attgtcttcg 9300 aatttatttc atttttttta ctcgttgcgt ttgtctccaa atattaatta gtacattgtt 9360 ttttttcaac gttattcatc tccatcagcc tttgacttag tttaactgca attttccact 9420 ctgtaaaata aggcagcatt ttgtctattt tgccttcact cttattcatt gtgttatcat 9480 acccaaagtg cgacaaatta acatccacaa aaaaaacggt ggtgttgaat atactacaat 9540 actgtgcact acattggata acctaccttt gaaagttttg ccattaacca caacacatct 9600 tgggaagcca catcgcaaga gataatcgcg gtggaagaaa acttttgtac ttattctata 9660 tacagataaa tccggtcagt ttggacgaaa tctcattatt aatcttacaa aatgggcctt 9720 gagcatctcg aagaattcag ttatcctaag gaacatggtg aagaagtcga gtatgatagc 9780 gaacaaggtg ttcgtaaaat ttatgttaaa tctattaaag aaacctttaa ctttgataat 9840 gtaagtgaag aagagaaaca ggaaggaggc gactactact tgggcaaaaa ggaagatgag 9900 cttgatgaag ttgtgcttcg ccctaatcct caccgctttg ttttgtttcc aattaagtat 9960 cacgaaattt ggcaattcta caagaaggct gaagcatctt tttggactgc tgaggaaatt 10020 gatctttcga aagacttggt tgattgggat aacaagttaa atgctgatga acgttacttt 10080 atttccactg tactcgccta tttcgctgcg tcagatggta ttgttaatga gaatcttctc 10140 gagcgtttca gctctgaagt tcagatccct gaagctcgtt gtgtttatgg tttccaaatt 10200 atgattgaaa atattcactc tgagacctac tcacttttgc ttgatacata cattcgtgaa 10260 cctaaggaaa aacagcgtca tttcgatgct attttaacaa tgggttctat taaggccaaa 10320 gccaagtggg ctcttcgttg gattaatgat gaagattcca cctatgcaat tcgtttggtt 10380 gcctttgctg ctgtagaagg tatcttcttt tctggatcgt ttgcctctat tttttggctt 10440 aagaaacgtg gacttatgcc tggtcttact ttttccaatg aattgatttg tcgtgatgag 10500 ggtcttcaca ccgactttgc ctgtttgatg tttagtcatt tgaagcacag accaggccgc 10560 aaggttgttg aagccattat tgttgaagct gttgatattg agaaagaata cttcacggac 10620 gctcttcccg ttagtctgct tggtatgaat aaggatttga tgtgtcagta tattgaattt 10680 gttgctgatc gtttgttggt tgctttgggc aatgacaagt actataacgt aaccaatcct 10740 ttcgatttca tggagaatat ctctttagca ggaaaaacaa atttctttga aaagaaagtt 10800 tctgactacc aaattgctgg cgttatgtcg ggtaccaagc gcgctgagaa ggatgatcat 10860 acatttacaa tcgatgagga cttttagatg actagttatt tttggttttt agataagtta 10920 aagcaaaaaa aaaatattat tgattactga taacctattt agcaataata aattaatatg 10980 tttaattaga ttttcgttac tctatacttt acaaatctaa atggctactt cagtgaatgt 11040 aatgacgaag ttctaatttc tgaagtattt ctattcaagt tcttttctct ttttcattaa 11100 tttactacgt ctaagaaaag ttattttcca gtcatagact aaaagaccat ttacttatta 11160 tccttattgt caattaccta aacctacaag attacatgta ttatacaata ctttgtaatt 11220 cgatttatgg ttgacttatc acatctcgta tttttttaat gaattactgc aaatttactc 11280 tcaaaaataa tcaatacttt cttttttttt aacgattatt tttctttaat tcgtattaaa 11340 tgtatgaagc caatatgcta agattaagtc aaaggatctc aaatgctgtg aatcacttct 11400 caaccgccat gatgtcgggg gcttctgcaa gtttgaccga gggtagtgca attataccat 11460 tttcaaatcc gaatgaagtc ttttataacc ccgtacaaca atttaataga gatttaagcg 11520 ttacagctat tcgtgcttgg tcggaaacta gatccaaaaa aattgttgca aaatctcatc 11580 accggcatca gcttctcgat caaaactccg aacttagcca agaaaatctt actaaatgtg 11640 atacaactca tgattttgaa aaaaactgtt cggaaaagac agattctact tcagctgata 11700 atattgctgg atttaccatt ttagaagcac tttccgctac tggattgcgt tccattcggt 11760 atgctaagga gttgccaaat gtcaaaagga tacttgcaaa tgatttatta gaaaatgctg 11820 tgaaaaccat cgagaagaat gttaattaca ataatgtgag cgatatagta attcctaata 11880 aaggagacgc taatgctgtt atgcatatga acaaatttca ttacgatgtg attgaccttg 11940 atccttatgg atctgctgcc ccgtttctgg atgctgctgt acaatccgtt tcaaaggacg 12000 gtcttctatg catcacatgt actgattcag ctgttctagc cggtaatgca tatccagaga 12060 aatgcttttc taattatggt gggtcctctt tgcgctctaa tttttgtcat gagcaggctg 12120 ttcgtcattt gctttatgct attgcagctt ctgctgcaaa atatggtcgc gctatcaagc 12180 cgctactttc cctaagtatt gatttttact ttcgcgtatt tgttcaaatt aaagcaaagc 12240 ctgttctcgt aaaaaattta caaagtcaat cgctattgat ataccattgt tcaggctgcg 12300 gtagctttgc cgagcaacca cttggaaaaa cttctccggg aagactacct ggaaccacca 12360 aattctctaa tgcttcgggg cctcctgtat ctgctaactg tgaacattgt ggatatgtgc 12420 atcatgttgg aggcccttta tggggtggac ccctacatga tgcagaattt ctcaaaaaaa 12480 tgcgcgccat tgcagaagat ttagaccctg aagtctatgg cactaaacga agaatcttag 12540 gtatgctcgc tttggctgat gaagaactac cagatgttcc attttatttt gttttatccc 12600 aaatttgtag cgtcttgcgc tcccaatctc ctcctcaaaa tatatttgtt tcagctttgt 12660 taaacgctgg atatcgggtt tctggttctc atgccaagag taatgcaatt aaaacaaatg 12720 ctccctggtc cttcgtttgg gatgttttgc gctcttggat taaagatcat ccagtgaagc 12780 ttgaaaatat tagtaaaaca tctgctggag cagcgatttt ggaaaaaact cccacagctg 12840 aggttgattt tacattcagg cccgattccg agtttgcatc caaaaaagaa ggttatactc 12900 gatatcaaat gaacccaact gaaaattggg gcccaaaaag taaacctggt aaaagaacta 12960 tcgctgaagt tgactcaaaa tcttaggtaa agtggagcac agtttaaata ctccttgtat 13020 tttaggtttt tggatagtac atacaatttc attctttatg ttatttcgga aggtttatgc 13080 aaattataaa caggctagta caaaaaagat ttagtttgtg tattccatac taaatacttg 13140 tttaaactaa tagtatccgc aacacaatta gcaatgttaa aatgatttat tgtttaaccg 13200 tattcttctt ttttacataa ttatttaaag aaggtgcaaa tattttctta attcttttgc 13260 ttcaacaatt tttggtattg ttactttcgt tggtggggta ctaactcgtc caacaacagt 13320 ttgctcatga aatgttgtcc tgcctcgata ttggatcgtt ttaatgcaat aaaagttata 13380 aatgtgatgg gattgcgtct aaacggacga agtgttcctt ggttgcttca gacacgaaaa 13440 tttatcaata tcactagtcc aagatacaca gctggcaggc ctgttatttt gaatgaaaat 13500 caactaatta gatacttacc aaattacaca aataaaggag atgaagaggt aacccgaagg 13560 aagaaattgc atgaacccaa tggaccaaaa tttcatgaat taccccttaa tcaaaatata 13620 ttagatggat tgtctacaaa ttttgcagag tataaaaatt caacgccttt gcaacaacgc 13680 ataatcaatg cgttaatgaa gagcggtgta tctttcattg tgcgaggttg gaatggctca 13740 gggaaatcgt ttggagctat gctgagtaca ttatcgatgt atttcgaggc tttaaaattc 13800 ctcaagcata atcctgtggc aggaaaaccg gttcaatcgg gatgtcaagt tttattcatt 13860 gtacctaatg ataatctggc agcacaatac cagttttgga ttgaacgttt attacatggg 13920 attacagaaa aagaagagtt gcaacatatt tataaaattc taacgctaac gccggcttcg 13980 ttagattctt ttcaaaacag gccaccatat ataggaatca ctacattccc aaatttacag 14040 cattgcataa aaaaaaatca accaatctta aaacaattta cttcacaact tcaattatta 14100 attgttgacg aatcagattt agtaattccc gatcacggca gatcacgaag gctctctagt 14160 ccagagattc tagctgacaa aatctttctg tcgtcgttta ttcaattgtg taaaaagaat 14220 ttgaggatta atatggatga cgaaaatgtc ggtggtatgg ggattcactc tcaattacat 14280 ggcaaaggct caattcctac aatggtattt atgtctgcat ctaatagtaa aaatggtgtt 14340 aattatttaa gtcgatatat aacagatcaa ctcgggataa ttgggatcga tagaaaacat 14400 caatggtatt ggaacttaac cagtactcca tcagtttttc attatctcat aaaagctcaa 14460 gctgaaccaa aaacaacaaa ggtcactttg acaaatttgc catatgagtt tgttcgattc 14520 aataattcta tgcatagtat tggtgacaac aatttttcat acaacaattc agaggctgtc 14580 ttacaaatat ttgcacaatc agtacctcga atattaaata tagaagccaa gaagtcaaaa 14640 ctcgagaata acattttaaa gtgtgttatg attgtacttc ccaaagagtt taatcctcac 14700 tctttttgca accaatataa agggtttgct gtgggtaacc gtttttggca atgtcgaaca 14760 ctgtcgactg aatcgctaaa tttttctact aaccttaata acgttgtatt tgttgcaaac 14820 ccttctgaaa ttcgaggcct acatttaccc tcaattacac acatatttca tctttggagt 14880 actttttctg gtgttgctta tcagcatata gctggaagac ttggagcaat gggtcaaaca 14940 ggaaagatct ttaatttcat tattccggac tactataaaa gcaatgattt taaaaaaaca 15000 gatttccgtc gttgcattct ctttatgcta caaagggtgg gaatccgacc gaaaagttat 15060 gaatttgacg acgaacactt ttaaattcta ataggactta gaacaatgaa aattgcacat 15120 ccgataaata cttcgtaaaa ttttcccaaa aaataattta tagaggatcg accgttcaca 15180 aaaattttaa tagtagtggt acgtatcaaa caacgacata ataaagcttt caaactggaa 15240 atttgttttc acttttcttt tttattcagt aggccacaat ttgcgggatt aaggacgatg 15300 tctataggag tgataggggg gtgattgatc agaatattca gatatattgg aacctggaga 15360 tcggctacgt gcccgagaat atctggagcg atgaaacgat ttatcgttgt aatgtgaatt 15420 gttttgtgac cttgatggtc gatacgagtt ttcgtagtgc ttatggtttt tgtgtagtga 15480 ctcaaaagga aaacgtttta tcgatacaaa caactcctca ccatcaagct cagcgttgtt 15540 catcttatcg acggcgtttg cggcctggtc atggtatacg tattctatgt agcaataccc 15600 tttattcaat tcagctgcaa agttagtggg tgtaattcat aaaaaataaa agctcactca 15660 cattttttat aaattggcat gaaaatatga tcaattagtc cataaatccc aaatatttct 15720 gctatatgtt ctttagtgac attacgcgtc aaattttcca caagaatcta ttttgcatcg 15780 tattagaaac gattagtatg ccttaaataa ctaattatac atggaaaacg tactgttcgc 15840 aatggttctt ctggagatct ggaaagcgaa ctcgagtaag atctattcga ggaaaaagaa 15900 tcacgactac tagaccgatg tgagaa 15926 //