ID SPBC243 standard; DNA; FUN; 2825 BP. XX AC AL133362; XX SV AL133362.1 XX DT 08-DEC-1999 (Rel. 62, Created) DT 08-DEC-1999 (Rel. 62, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c243. XX KW mph1; Serine/Threonine protein kinases active-site signature; KW yeast spindle checkpoint protein kinase Mps1 homolog. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-2825 RA Wood V., Rajandream M.A., Barrell B.G., Quail M., Skelton J., Churcher C.; RT ; RL Submitted (06-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O94235; O94235. DR SWISS-PROT; O94235; MPH1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c243 is overlapped at the 5' end by cosmid c1271, EMBL CC entry SPBC1271, accession number AL034353 and at the 3' end by CC cosmid c106, EMBL entry SPBC106, accession number AL110295. XX FH Key Location/Qualifiers FH FT source 1..2825 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /map="IIL" FT misc_feature 1..417 FT /note="nominal overlap with SPBC1271 S. pombe chromosome 2" FT CDS 318..2354 FT /db_xref="GOA:O94235" FT /db_xref="SWISS-PROT:O94235" FT /label=mph1 FT /note="SPBC243.01, len:" FT /gene="mph1" FT /gene="SPBC243.01" FT /gene="SPBC106.01" FT /gene="SPBC1271.16c" FT /product="yeast spindle checkpoint protein kinase Mps1 FT homolog" FT /protein_id="CAB62431.1" FT /translation="MSKRNPPVTNIADLVSDSSLDEDSLSFLEELQDPELYFKNDTFSS FT KSSHSDGTVTGDTLRRQSSGATALERLVSHPRTKNFDLQGNGGQNSALKEVNTPAYQSM FT HHFEHLITPLPSTNASHSEVSLSAGVNDLNSNSEHDLLPKSVNKTPGSLSISRRRRIGR FT IGLGPPKRAEYTLTDPSKTSDTKNSTEADEDIEMKSREVSPASNSVAATTLKPLQLHNT FT PLQTSQEHPKPSFHPSQFESSFSPRVQFDHDVERRASELHSRPVTVFQEPQRSASQPYE FT SHALSPKVAPLFDNSQATPIPKRQQDVVTVANLQFIKLGVVGKGGSSMVYRIFSPDNSR FT LYALKEVNFINADQTTIQGYKNEIALLRKLSGNDRIIKLYAAEVNDTLGQLNMVMECGE FT TDLANLLMKNMKKPINLNFIRMYWEQMLEAVQVVHDQNIVHSDLKPANFLLVEGNLKLI FT DFGIAKAIGNDTTNIHRDSHIGTINYMAPEALTDMNAHTNSGVKLVKLGRPSDVWSLGC FT ILYQMVYGRAPFAHLKMIQAIAAIPNEQYHIHFPEVALPANAVQEKEGSLPGVTVGPDL FT MDVMKRCLERDQRKRLTIPELLVHPFLNPLPSYLTPLAKKPLPVSGHTNNAHPLRLSTE FT ISASQLSMIIERSVELSKHKRLNKELIDSMAYDCVSNLRKMPE" FT misc_feature 1263..2126 FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 203.71" FT misc_feature 1281..1352 FT /note="PS00107 Protein kinases ATP-binding region FT signature" FT misc_feature 1629..1667 FT /note="PS00108 Serine/Threonine protein kinases active-site FT signature" FT misc_feature 2073..2825 FT /note="nominal overlap with SPBC106 S. pombe chromosome 2" XX SQ Sequence 2825 BP; 864 A; 551 C; 537 G; 873 T; 0 other; aaaaaacttt ttactttcac aactcttctt tttctttcat gactagtcta ataatgatct 60 tatgccattc ttactcagaa ttttcgtttt cataaccatg cgaaactcgt aaaataatga 120 tatacactga agtagctagc attgcgttag agggtgatct ttgtagtaac ataacatttt 180 gaaatgtgca cactaatggt tgctctgctg ttcgtctttt ctagctattc ttataacgaa 240 gtaaaaattt attgattaac gcttaacaaa cagttggaaa ttcggtttgg aatgctcgtt 300 ttttagtaac tgtgtttatg tctaagcgca atcctcctgt gactaatatc gcggacttgg 360 tgtcagattc ttccttagat gaagactcgc tttcttttct cgaagagctt caggatccag 420 aattatactt caaaaatgac actttctctt ccaagagtag ccattctgat ggcaccgtta 480 ctggggatac gttgcgtagg cagtcaagcg gtgcaactgc tttagagaga ttggtctcac 540 atcctcgtac taaaaatttt gatttgcaag gaaatggagg acaaaattct gctttgaagg 600 aagtgaatac tccagcatat cagtcaatgc accatttcga gcatttaata acacccttgc 660 cctctactaa tgcgtctcac agtgaagttt cactcagtgc aggagtgaat gatctcaatt 720 ctaattcgga gcatgatttg ttacctaaaa gtgtaaacaa aacccccggt tctttatcaa 780 tttcaagacg acgaagaatc ggcagaattg gattaggccc tccaaagcgt gctgagtaca 840 cgttgacgga tccctcgaag acttccgata ccaaaaactc tacagaagca gatgaggata 900 ttgaaatgaa atctcgagaa gtatcaccag cttccaactc tgttgctgca acaactttaa 960 aacctctgca gctgcataac actcctttgc aaacatccca ggagcatccc aaaccttctt 1020 ttcatccttc tcagtttgag agctcttttt ctcctagggt gcagtttgat cacgatgttg 1080 aaagaagagc tagtgaactt cattctcgtc cagtcaccgt tttccaagag cctcagcgtt 1140 ctgcttctca accatatgaa tctcatgctc tttctccaaa ggtggctccg ttatttgata 1200 acagtcaagc tactcccata cccaagcgtc agcaggacgt tgttactgtt gccaatctac 1260 aatttatcaa attaggagtt gttggaaagg gtggaagtag tatggtatat cgcatatttt 1320 cccccgataa cagtcgttta tacgctttga aagaggtgaa ctttattaat gcagaccaaa 1380 ctactataca aggatacaag aacgaaattg cattattaag aaagctttca ggcaatgatc 1440 gcataattaa attatatgct gccgaagtta atgatacttt agggcaactc aatatggtga 1500 tggaatgcgg agaaacggat ttagcaaacc ttttaatgaa aaacatgaag aaacccatta 1560 atcttaattt catcagaatg tattgggagc aaatgctaga ggcggtccag gtagttcatg 1620 atcaaaatat agtgcattcg gatttgaagc cggccaattt cctgcttgta gaagggaatt 1680 tgaagctgat tgattttggc attgccaaag caattggtaa tgacaccact aatatccatc 1740 gtgattccca catcggtact attaattata tggcacctga agctttgaca gacatgaatg 1800 ctcacacaaa ctctggcgtg aaactcgtaa agttgggcag gcccagcgac gtgtggagtt 1860 tgggatgtat attatatcag atggtgtatg ggagggcccc gtttgctcat ctaaaaatga 1920 tccaagctat agcagctatc cctaatgaac aatatcacat tcatttcccc gaagttgcct 1980 tacctgctaa tgctgtccag gagaaagagg gatcgttgcc aggtgtaact gtcgggcctg 2040 atctaatgga tgttatgaaa agatgcctgg aaagggatca acggaagaga cttacaatac 2100 cggaattgct ggttcatccc tttttaaacc ctttgccatc ctatttgaca cctttggcca 2160 aaaagccgtt acctgtttct gggcacacca ataatgctca tccacttaga ctcagcacag 2220 aaatctcagc ttctcaatta tcaatgatta tagaaaggtc ggtggagttg agtaagcaca 2280 agcgattaaa taaggaactt attgatagca tggcttatga ttgcgttagc aatttacgaa 2340 aaatgccaga atagaggcac taaattttcc gattattctt aaaaaggttt tatcttttac 2400 aaagtttccc aaacatcata cgagtttctt aatgttaacg acttttttag ggagcttaaa 2460 ggcttttttt ttttaatctt taatttttcg ctcttttaaa agaaggtaat tatgaccttg 2520 attaatgaac cacatattat atggcccctg atatctatac tttgatacta tgtaaatata 2580 aagaacgcta tactcccaaa cgctataagt tgtttgttta cgatataata cctttttgtg 2640 tttgtttttt ttaggtaaag caaccaaatg aatgatgctt ttttataaga aactttttta 2700 atataattgg gttatattag acattaaaaa aaaaccccat aactaatgta aaacaaaaac 2760 ctttaaaaat aatgtaccgc cggtagagat gattattaac gccataaatg ttggtgcagg 2820 aaggg 2825 //