ID SPBC21 standard; DNA; FUN; 15041 BP. XX AC AL035537; XX SV AL035537.1 XX DT 24-FEB-1999 (Rel. 59, Created) DT 24-FEB-1999 (Rel. 59, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c21. XX KW cdc7; cell division control protein 7; KW Mitochondrial energy transfer proteins; ral2. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-15041 RA Saunders D., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (23-FEB-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk. XX DR GOA; O60165; O60165. DR GOA; O94645; O94645. DR GOA; O94647; O94647. DR GOA; P15258; P15258. DR GOA; P41892; P41892. DR SPTREMBL; O94643; O94643. DR SPTREMBL; O94644; O94644. DR SPTREMBL; O94645; O94645. DR SPTREMBL; O94646; O94646. DR SPTREMBL; O94647; O94647. DR SWISS-PROT; O60165; SPN7_SCHPO. DR SWISS-PROT; P15258; RAL2_SCHPO. DR SWISS-PROT; P41892; CC7_SCHPO. XX CC Notes: XX CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. XX CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. XX CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). XX CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. XX CC The length in codons is given for each CDS. XX CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. XX CC Cosmid c21 is overlapped at the 5' end by cosmid c776 CC and at the 3' by cosmid c19F8, AC AL023594. XX FH Key Location/Qualifiers FH FT source 1..15041 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c21" FT /map="IIR" FT misc_feature 1..123 FT /note="nominal overlap with SPBC776 S. pombe chromosome 2" FT CDS join(1..45,86..1145,1195..1401) FT /codon_start=2 FT /db_xref="SPTREMBL:O94643" FT /label=SPBC21.01 FT /note="SPBC21.01, len:436, SIMILARITY:Schizosaccharomyces FT pombe, D1012049, map1., (398 aa), fasta scores: opt: 149, FT E():0.092, (18.4% identity in 272 aa)" FT /partial FT /gene="SPBC21.01" FT /product="hypothetical protein" FT /protein_id="CAB36881.1" FT /translation="HNVDERRAARMRGAERYKIQNVEFSLDQNLLANLESSTSSSTRSS FT PASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERRISEEVVRENSVSSIENV FT SSSAVARETQSSANILVKDNHFFSHKQKKIQRESIPFHKRDNIETSDAYSSSILENSPP FT NKVQRLSSLDSSQDSFQEEHPGNVTGTTFSSQAPEERIASPISTSSPESLTNQSSSLQS FT SLQTSSMAPRNLLDQSTEKDIVVGASDELVRIELTKLSKEAGGSKTLNELDAVQQFFQE FT FIKENEPLEPYVVKVKNAFVEQVSIRLLELIDLLDANRILSTACKKAANEKLAVQRDLS FT KLREDRLSVQRKKIQLRNEYIKLSHQQNFLDDIDDFFSQCETVKNELENVTTTPNSTEA FT FSEINEYASALSKNYGLESQLVELQSLLTQFYQKFLS" FT misc_feature 46..51 FT /note="gtatgt, splice donor sequence" FT misc_feature 73..85 FT /note="ttaaccaatgtag, splice branch and acceptor" FT misc_feature 1146..1151 FT /note="gtacgc, splice donor sequence" FT misc_feature 1181..1194 FT /note="ctaacttaatatag, splice branch and acceptor" FT CDS join(1985..2105,2158..2218,2276..3629) FT /db_xref="SPTREMBL:O94644" FT /label=SPBC21.02 FT /note="SPBC21.02, len:512, SIMILARITY:Saccharomyces FT cerevisiae, Q12108, yor3263w from chromosome xv., (567 aa), FT fasta scores: opt: 340, E():2.1e-15, (25.4% identity in 338 FT aa)" FT /gene="SPBC21.02" FT /product="hypothetical protein" FT /protein_id="CAB36882.1" FT /translation="MGQKESQFKVLWQLQTHEEYMKQTSSFLRDFNALERYNLKENFEA FT LSSFDNGEKIWVEDAFTTFFGIPDVIQLSPFFYRSACSFGRDLNINRQRLTFSALARFL FT ASYTGRHKDVWSDFESNLMIIASWCDSFPKFRVEVLKNSKQISRLIHSDVQNISNFFDQ FT NFGIRRSNIYQLCLLLLCISKLKPGESVGCHINSFLDSMFVKEQKAAFYVLQGISPDIS FT SNIIKPSYLLHFLESNPYFLKSLGSLFELALFPHSAHNQKVQDDVSPLNDFAILSPLIH FT AQICFFLPKSVWQVNGLTSLFRASFHGYSMYALERKMCNYHNPSILLIKAKKINANHKS FT SSRPISLDATIPRKYPPHCIGTDKAVPQKFGADFHNENILLGAYISTRWRQSHMGFFGD FT HSTLLFQLQPIHQVYYASNLDKNYCMFDKNVGLGFGLSRHKVTNKVQYDVPGVCMYIDD FT GLEYGLFRHAGDGAFKPATNYENFEYEERFLIQDLEVIGVDTTKPVEPIHIGL" FT misc_feature 2106..2111 FT /note="gtttgt, splice donor sequence" FT misc_feature 2139..2157 FT /note="ctaatgatttaataaaaag, splice branch and acceptor" FT misc_feature 2219..2224 FT /note="gtatgt, splice donor sequence" FT misc_feature 2258..2275 FT /note="ctaacaaaggtgctttag, splice branch and acceptor" FT CDS join(complement(4409..4663),complement(4183..4207), FT complement(3701..4140)) FT /db_xref="GOA:O94645" FT /db_xref="SPTREMBL:O94645" FT /label=SPBC21.03c FT /note="SPBC21.03c, len:240, low SIMILARITY:Gallus gallus, FT Q90679, thymocyte protein cthy28kd., (242 aa), fasta FT scores: opt: 409, E():1.5e-18, (38.8% identity in 170 aa)" FT /gene="SPBC21.03c" FT /product="hypothetical protein" FT /protein_id="CAB36883.1" FT /translation="MTEDAEKRRYWLMKAEGEPRYVKGINVAFTFEMLEEITEDGKMES FT WSGVRNYEARNMIRDEIKIGDYAFLYCSNCKFPHIKGLCQICSNSHPDDSAWNSNDPYY FT DPKSTPQNPRWYSVGVQSEYKLDRPVTLRELKMHKENQLKSMELLNRSRLSISRVKPEE FT WKFIHELSKQPEPEEIIKARLQEEEKVAKRKRKLNLSDGENKAKSPYSSISENDASLDI FT IKRSRIKDVRSQKDNAL" FT misc_feature complement(3866..3895) FT /note="PS00215 Mitochondrial energy transfer proteins FT signature, P.[DE].[LIVAT][RK].[LRH][LIVMFY][QMAIGV], info FT count = 17.9" FT misc_feature complement(4141..4153) FT /note="ttaacagttttag, splice branch and acceptor" FT misc_feature complement(4177..4182) FT /note="gtatag, splice donor sequence" FT misc_feature complement(4208..4222) FT /note="ctaactatcttgtag, splice branch and acceptor" FT misc_feature complement(4403..4408) FT /note="gttatt, splice donor sequence" FT CDS join(5320..5447,5585..6059) FT /db_xref="SPTREMBL:O94646" FT /label=SPBC21.04 FT /note="SPBC21.04, len:201, SIMILARITY:Saccharomyces FT cerevisiae, YB43_YEAST, hypothetical 25.3 kd protein in FT rim2-msi1 intergenic region., (223 aa), fasta scores: opt: FT 275, E():2.9e-11, (30.4% identity in 191 aa)" FT /gene="SPBC21.04" FT /product="hypothetical protein" FT /protein_id="CAB36884.1" FT /translation="MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPT FT IHKNFNILLSQIHSLSNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVE FT EWEANTLQEYEASISSQPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQES FT EGEFVSQRQLEIDRATEEQNANQMLTDILSFMKSGKR" FT misc_feature 5448..5453 FT /note="gtatgt, splice donor sequence" FT misc_feature 5568..5584 FT /note="ctaatatttatacacag, splice branch and acceptor" FT CDS complement(6062..7897) FT /db_xref="GOA:P15258" FT /db_xref="SWISS-PROT:P15258" FT /label=ral2 FT /note="SPBC21.05c, len:612" FT /gene="ral2" FT /gene="SPBC21.05c" FT /product="ral2 protein." FT /protein_id="CAB36885.1" FT /translation="MSEVKRNISLRNSTNIFTSTFSLSSNNVPKPLIGESVIKYGDEAF FT VYGGRDALNAQLVNDMYVVDLNTCSWKQVEYQGNQKPIPRYFHSGDLWNNKLIFFGGMG FT FNDDTKCLYVLNDIDIYDIETKQWSHIPGMITENQTNDDAKEVNGSDVDEKSKHLYPSA FT RYGHLHCVLDHYLIIFCGQDLSNSYIEEINIFDLDSGKWVFKSLFNHHCGIYRSNCVVI FT NKDSEFLQMCRPINTTQDSNEHSIGSLFFYLNYNFVNVKRQVIYLELFELDTAESEKKS FT AALAKDNNQSFRFLELDVTEKFLSSAMPPGLRFPAVNILGDNLILSGIYLTSSRQAFVL FT WVYSLDKELWLQLDMLGVLNHGSWFKCLVLDCTNRFVVFGNKTRKLTQDYNLRQSNYDH FT IVFIELEGYGVYRKPQMGRVTERSEQLGKLLLNGISDMEILTIERMHIPCLSRMLYKRW FT PAFQKIMDRAVEKNQEAFQAEVSQLGPQLTDLPFSSIHSTGSRALYMPYSFETCSAFLH FT YIYCGTLNGSYCTAKNLCNLLILCKGFEGLETFFAYIVHLLHGVLNRNNVKLIYETAAL FT TGAKGLQLRALRRIARIEQGGTAISPTSPLPNLDD" FT CDS join(complement(12120..12146),complement(11933..12067), FT complement(11862..11891),complement(11748..11812), FT complement(11447..11700),complement(10935..11396), FT complement(10653..10894),complement(10521..10584), FT complement(10131..10483),complement(9955..10089), FT complement(8497..9918)) FT /db_xref="GOA:P41892" FT /db_xref="SWISS-PROT:P41892" FT /label=cdc7 FT /note="SPBC21.06c, len:1063" FT /gene="cdc7" FT /gene="SPBC21.06c" FT /product="cell division control protein 7" FT /protein_id="CAB36886.1" FT /translation="MHNIQASSITLGDCLGKGAFGAVYRGLNIKNGETVAVKKVKLSKM FT LKSDLSVIKMEIDLLKNLDHPNIVKYRGSYQTNDSLCIILEYCENGSLRSICKNFGKIP FT ENLVALYTFQVLQGLLYLHNQGVIHRDIKGANILTTKDGTIKLADFGVATKINALEDHS FT VVGSPYWMAPEVIELVGATTASDIWSVGCTVIELLDGNPPYYDLDPTSALFRMVKDEHP FT PLPSNISSAAKSFLMQCFQKDPNLRIKTRKLLKHPWVIMNQTSSKFSDAIDEVQKYNER FT VKESTLTAIIEPTSNRINPTLHSGRQSSYHMPESPKTPIAESPDHDNWDNEFQGTLKIS FT DDVLKKSEHFMDFCSNFKGKNNSSSITSSPSKSRHAFNSDQISESNNFNASPLSTPLKA FT QFDPSKPALNRSIDHQKTPQHKRYLSTEFKENIPDGIEKFVETPRDSEFTDIFPTSSIK FT VQGLRKETGLGTLVLNKCYGSWNNEENEDGEESDIFDSIETNLENLDIENNIALDKRTH FT LASLLSSLLGSLRDKNIGSKDTTVSQIASILSEDLSLKREIIQAHGILPLLETLREIKT FT PDVQLLLLKLINTVAFDDHTTLQKVCFAGGLPLMLSFSNREHSFEFRYESAIFIQQMYR FT TSALTLQMFLSSNGLNSLLLFIKEDYGTNRDFVFVGVEGIWKLLRQQDYIPKNDICTMV FT VNDSLEPLTKAMLKALATDDDSSRMSLTRICEILLALSQADNYVKESLLCESALRRILR FT ILLYLPHSDMAITLQFFKQLSMVPSSLSLLRKVHIIPLLTHILGDSKIEKGRKEIRSEA FT LAALFNVCKLDKKSQEEAVISGAIPLLQEVIIKDRLFKEFALPILLALPQAGPVSRIYL FT WQNKCLDFFLSLLSDLNWQSAVFDTIASWLQFELREVQRVLAEKRNVQLVLKVFCISQS FT ASSNRMLDTLGRVCQISPRLAASYGQPIIFQKFKEKLTHKGTKPIVVLNIFQIMKSMCE FT ASSQSVAYIAHCGLPDVVANLNQTSDSVLVKELAKDLLKYLKVPQGPINEHKSPISKPH FT MPPPRWQPKQPLTQ" FT misc_feature complement(9919..9928) FT /note="ctaacgttag, splice branch and acceptor" FT misc_feature complement(9949..9954) FT /note="gtaagg, splice donor sequence" FT misc_feature complement(10090..10103) FT /note="ttaactgacaatag, splice branch and acceptor" FT misc_feature complement(10125..10130) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(10484..10495) FT /note="ctaattatctag, splice branch and acceptor" FT misc_feature complement(10515..10520) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(10585..10600) FT /note="ttaaccttttttttag, splice branch and acceptor" FT misc_feature complement(10647..10652) FT /note="gtaaat, splice donor sequence" FT misc_feature complement(10895..10907) FT /note="ctaacatttttag, splice branch and acceptor" FT misc_feature complement(10929..10934) FT /note="gtagct, splice donor sequence" FT misc_feature join(complement(11933..12070),complement(11862..11891), FT complement(11748..11812),complement(11447..11700), FT complement(11131..11396)) FT /note="Match to PF00069, eukaryotic protein kninase, Score FT 264.26" FT misc_feature complement(11397..11412) FT /note="ctaacaatttatatag, splice branch and acceptor" FT misc_feature complement(11441..11446) FT /note="gtaagc, splice donor sequence" FT misc_feature complement(11701..11714) FT /note="ttaacgatatatag, splice branch and acceptor" FT misc_feature complement(11742..11747) FT /note="gtacgt, splice donor sequence" FT misc_feature complement(11813..11825) FT /note="ctaaacgtggtag, splice branch and acceptor" FT misc_feature complement(11856..11861) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(11892..11905) FT /note="ctaacaatttttag, splice branch and acceptor" FT misc_feature complement(11927..11932) FT /note="gtatga, splice donor sequence" FT misc_feature complement(12068..12080) FT /note="ataacatattcag, splice branch and acceptor" FT misc_feature complement(12114..12119) FT /note="gtatgc, splice donor sequence" FT CDS join(complement(13070..14003),complement(12536..12987)) FT /db_xref="GOA:O94647" FT /db_xref="SPTREMBL:O94647" FT /label=SPBC21.07c FT /note="SPBC21.07c, len:462, SIMILARITY:Schizosaccharomyces FT pombe, O74526, hypothetical 85.9 kd protein., (781 aa), FT fasta scores: opt: 654, E():1.1e-32, (29.9% identity in 431 FT aa): N terminal prediction may be incorrect" FT /gene="SPBC21.07c" FT /product="protein kinase" FT /protein_id="CAB36887.1" FT /translation="MTNYPFFRQGTIFVDNSAIQRNSENKNSLSIENIFGRFPKEFFQF FT FSINVSKSTTKKSSVVIKPSTITAPWLENEYLDSNTSLLSVHSIQPSFIGMSDFAIGLD FT PSLKRILPEIRFRLDFQHLKSIAKGATSTIKVVTHRDKITDAKIYYAAKVYRKTKTSHK FT KRLNTMVYFLREWSIQPKLDHPNILKVICPCVTLTSVFNKSAGFCLVQEYCPQGDLFKQ FT IEEKVLTLEDKCCYLKQILQAVAYLQSQRIAHRDLKPENILIGRDGLLKLTDFGTSEIV FT GNPGDNESIRFVSGAVGSLAYLAPEAFHENEYCGLLADRWSCGILLKVLFTGYFPFKTS FT VKTDLYYSKYMSILTDTCGISSTDESSFQTEVIKQIPTLQPLRYIPEGAKKIILSLLNP FT DSQNRPSLDSILGTAWVRKLDCCSNFSTDHENKSLQEVDFDASKPITRKSLIPRIHNHQ FT TLV" FT misc_feature join(complement(13070..13646),complement(12674..12987)) FT /note="Match to PF00069, eukaryotic protein kinase, Score FT 174.08" FT misc_feature complement(12988..13006) FT /note="ctaatagttcaattgatag, splice branch and acceptor" FT misc_feature complement(13064..13069) FT /note="gtaagt, splice donor sequence" FT CDS join(complement(14919..14931),complement(14596..14876)) FT /db_xref="GOA:O60165" FT /db_xref="SWISS-PROT:O60165" FT /note="SPBC21.08c, len97" FT /gene="SPBC21.08c" FT /product="hypothetical protein" FT /protein_id="CAB36888.1" FT /translation="MRTNVSDSTKSEELKKINSIKVDNTSSLKCDSYGNTKTKTNQLNC FT EQIGLEVISPKEFPHRTTSSRNSLPNNTTKELEMKKMDDLSHERYENLPFYR" FT misc_feature complement(14877..14889) FT /note="ctaatgaacgcag, splice branch and acceptor" FT misc_feature complement(14913..14918) FT /note="gtaagt, splice donor sequence" FT misc_feature 14940..15041 FT /note="nominal overlap with SPBC19F8 S. pombe chromosome 2" XX SQ Sequence 15041 BP; 4911 A; 2713 C; 2541 G; 4876 T; 0 other; ccataacgta gatgagcgac gagcagcacg aatgcgtgga gctgagtatg ttatttcaaa 60 actttgtaaa aattaaccaa tgtagacgct acaaaattca aaatgttgag ttttcattag 120 atcaaaacct tctggccaat ttagagtcat ctacttctag ttcaactcgt tcttcgcctg 180 cttcaaacca acaaaacctt gaaattgtat ctcaatcaga ggcatatcat aaaattagcg 240 atttggatga cagtacatct attttttcta tttccgatca agaaaatata cttgaaagaa 300 ggatttctga agaagtagtt cgggaaaatt ccgtttccag catcgaaaat gtatcttcat 360 cggctgtcgc acgtgagacg cagagtagcg caaatatttt agtcaaagac aatcattttt 420 tttctcataa gcagaaaaaa atacaaaggg aatccattcc gtttcacaaa cgtgataata 480 tagaaacctc tgatgcatat agttcttcaa tcctagaaaa ttcaccacct aacaaagtac 540 aaaggctgtc atctctcgac tcatctcaag attcgttcca agaggaacat ccaggcaatg 600 tgacaggaac aacatttagt tctcaggccc ctgaagagcg aattgcttct cctatctcca 660 cttcttctcc ggaatctttg acgaatcaaa gtagtagtct gcaaagttcc ttacagacat 720 catcaatggc tcctagaaat cttttggatc aatcaacaga aaaggatatt gtagtgggag 780 cctcagatga gctcgtgcgt atcgagttaa ccaaactttc taaagaagca ggagggtcaa 840 agactcttaa tgagcttgat gccgtacagc aattttttca agaatttata aaagagaatg 900 agccactaga accctatgta gtcaaggtaa aaaatgcgtt tgtggaacaa gtttctattc 960 gtttactaga gttaatagac ttgctggacg ctaatcgaat tttatcaaca gcttgtaaaa 1020 aagcagcaaa tgagaaacta gctgttcaac gggatctctc gaaattgagg gaagatcgac 1080 tatcagtgca acgtaaaaaa atacagttac gaaatgagta cattaaattg tcccatcaac 1140 aaaacgtacg cataacattg gctctttaat ttacgcttaa ctaacttaat atagtttttg 1200 gatgatattg acgatttctt ctcacaatgc gaaacagtga aaaacgaatt agaaaatgta 1260 accacaactc caaactctac cgaagctttt tcagaaataa atgaatatgc gtctgcttta 1320 tcaaaaaatt atggactcga atcgcagcta gttgaattac agtctttact aacccaattt 1380 tatcaaaaat tcctttccta aaagttgcca tatcgcaact tctccgctgt ctttctatca 1440 cctttttgta taatttttca tttcggtgcg tctgtttggg aaatcggctg gatttctact 1500 gtttcttaaa gttttgcttt tcttgtgttc taaactaccc tttatctttt gctaatgtga 1560 aagctttgat tgggtttttg tatataaaat tttttgaaat atgagtatat gaatatatga 1620 atgtataata aatggcatgt cattattacc ccaattttct atttcgacag catttccgtt 1680 ttaaattgta tgctgcataa ttttctagta gaaattcgac tttttcgtca ctaacttgaa 1740 ctgcttacac ttacaattga gcatgtattt ttttaacaag ttaggaaaat aaaaaaaaca 1800 aaaaaatgca aaggaatttg cttctaaatg gatatctttg tatatcgcgt ttgttttcaa 1860 tttagatgag tattaagtgc aatattagtt aacttgtaac ttcgtttagc acaactctat 1920 tttcatagaa tatgaagtca tattcactac actctgaatc acgccgaaga tacccagccc 1980 aaaaatgggt caaaaggagt cccaatttaa agttctttgg cagttacaga ctcatgaaga 2040 atatatgaaa caaacttctt ctttcttacg agactttaat gctttagaac ggtataattt 2100 aaaaggtttg tcaattttcg taattttgtt taacaaagct aatgatttaa taaaaagaga 2160 atttcgaggc tttatcaagc tttgataatg gtgaaaaaat ttgggttgag gatgccttgt 2220 atgttcgctt ttaaaggtat tgttgaaatt gttcattcta acaaaggtgc tttagcacga 2280 cgttctttgg aattccagac gttatccaac tttcaccatt tttttatcgc tccgcttgta 2340 gttttggaag ggatcttaat atcaataggc aacgtcttac attttctgct ctcgcgagat 2400 ttcttgcttc ttacactggt cgccacaaag atgtttggag tgattttgag agcaatttga 2460 tgatcatagc aagttggtgt gactcttttc caaaatttag agtagaagtt ttaaaaaatt 2520 ctaaacaaat ttcgcggtta atacatagtg atgttcagaa cataagcaat ttttttgatc 2580 aaaacttcgg tatacggagg tcaaatattt atcagctatg tttgctgtta ctttgcattt 2640 ctaaacttaa gcctggtgaa tcggttggtt gtcatattaa ttcgtttttg gactctatgt 2700 ttgtgaaaga acaaaaagct gccttttatg tgctacaagg tataagtccg gatatatcta 2760 gtaatattat aaaaccgagt tatttacttc attttcttga gtcaaatcca tatttcctta 2820 aatctctagg atctttattt gagttggcac tatttccgca ttcagctcac aatcaaaaag 2880 tgcaagatga tgtgtcaccg ttaaacgact ttgcaattct gagcccactt atccatgctc 2940 aaatctgttt ttttttaccc aaatctgtat ggcaggtcaa tggcttgaca agtctgttta 3000 gagcaagctt tcatggttac tcaatgtacg cactagagag aaaaatgtgt aattatcaca 3060 atccttcgat tctcttaatt aaagcaaaga aaatcaatgc caatcacaaa tcctcttcta 3120 gaccaatatc attagatgct acaattccga gaaaatatcc accacactgc attgggactg 3180 ataaagctgt gcctcaaaaa tttggtgcag attttcacaa tgaaaatatc ttacttggtg 3240 cttatatatc cactcgttgg agacaatcgc atatgggatt ttttggagac cattcaacat 3300 tattatttca acttcagcct atacatcaag tttattacgc ttccaatctt gataaaaatt 3360 actgtatgtt cgacaaaaat gttggcctgg gttttggcct cagccgtcat aaagtgacta 3420 acaaagtgca atatgatgta cccggagtat gcatgtatat tgatgatggt ctagagtatg 3480 gattatttag acatgctggt gatggagcat tcaagcctgc gacaaattac gaaaactttg 3540 aatatgaaga aagattttta attcaagact tagaggtcat aggagttgat actacaaaac 3600 cggtagagcc aatccacatc ggattataat ggacgcatat tggaaaaacg atcataaata 3660 ttagtatata tatataattt tttgtcaata agaaaaaaac ttataacgca ttatcctttt 3720 ggcttctaac atcttttatt ctgctccttt taatgatatc caaagatgca tcgttctcac 3780 taatcgaact gtacggactt tttgctttgt tttcaccatc agaaagattt aatttcctct 3840 tcctttttgc aaccttttct tcttcctgaa gacgagcttt gattatttct tcgggctctg 3900 gttgtttcga cagttcatga ataaatttcc actcttcagg ttttactcgt gagatactca 3960 acctcgatct atttaataat tccatgcttt tcagctgatt ctccttgtgc attttcaatt 4020 ctctcaacgt aactggtcga tctaatttgt actctgattg tacaccgaca gaataccatc 4080 tagggttttg cggagtagat tttggatcat aataaggatc attagaattc cacgctgagt 4140 ctaaaactgt taatgatcat tacattttaa aatacactat accatcggga tgagaattgg 4200 aacaaatcta caagatagtt agtacagtta taaataccac gaataattct tgtctattat 4260 aatagatata gaattcatgc tcaaagctct attacaattc tccgtattcg aaaaagttcg 4320 tctactagat atttgtaata aaaatgtgat gcttactctc atcaccccta atttataaat 4380 aaagttagct gcttgttcgg aaaataactt ggcacaaacc cttaatatga ggaaatttgc 4440 aatttgaaca atacaggaaa gcgtagtctc ctatcttgat ttcatcccga atcatgtttc 4500 gagcttcata gtttcgaaca ccactccatg attccatttt cccatcttct gtaatttcct 4560 ctaacatttc aaatgtaaaa gctacgttta tgccttttac atatctaggc tctccttcgg 4620 ccttcataag ccaataacgc ctcttttctg catcttcggt cattgcgcct attctttaga 4680 aaccaaaatg taaaaaaatg ttaagttaaa tataattacg tacgtatgaa ggttgttaag 4740 ttaaatgatg ggagttagtg ttataatttc aagtggatgt cttagacaca ctaatgtttc 4800 gacagccaga attccgtttt actaaattaa gacgtaccaa atattttttc ggcattttga 4860 aacaaagtaa agtattagtt gctatctttc tatattagaa atctaaaacg ttggagcatt 4920 tatagctact tacgctaccc gaagttccac ttttgtgaca attttattta agtagtttat 4980 gagattaaat atcatataga tttcatagac attaaaacta ttgaacgata tgaataacca 5040 tgtatacatt aaacagctgg aacgtatttt ctaaaacgtt tacgaactca tattattctg 5100 ccttaagtaa aatatgaggt attgcatacc acgaacacac atatgaattg taatttattc 5160 ctattgtaac aagttttagg ataaagaaaa agctcaactt cttttccgtt agatattaaa 5220 cgattacata tatattttga ggtgttgtta ttcgagtttt aacgctcgaa tactcattaa 5280 gtgttgaaat tgtgaataga ttagcaagct taaaaaaaaa tggaagacat atctaccgaa 5340 aaaacagtgg aatctttgga agcaatccga catagaatag cacaaattgt tcaatcgtta 5400 actcattttc ttgcaattct tcatcaaagc gaatctcttt caccatggta tgtgcacaaa 5460 tgaatgttct taattttatt ttttgaggat aaaagtgata gctccattat tctaagttaa 5520 agatatacga agaaaacgga tctgtaatca tttgtatttc aaccctacta atatttatac 5580 acaggcctac gatacataaa aacttcaaca ttttgctgtc tcaaattcat tctttaagta 5640 ataatttagc agctcattct cacactcttc aaacaactag catatacccg tctcttgaat 5700 tcccagttaa agaacaagag ccattattga ccaccctact ccgaacaaaa gctctccctg 5760 aagtagaaga atgggaggca aatacccttc aagaatatga agcatctata tcatcacaac 5820 ctaaaaagaa agaggctaac gacgcctatc aaaaggacca attatgggac caagcaagaa 5880 tcatatttat ggaagaacga gaaaattata gctggtttga ttttgtaacc cgaaggcagg 5940 aatcagaagg agagtttgtg agccagcgac aactagagat tgatagagct acggaggaac 6000 aaaatgcaaa tcaaatgtta acagacatac tatcattcat gaaatcaggt aaacgttaag 6060 gttaatcatc taagtttgga agtggagaag tgggtgagat tgcagtgcct ccttgttcaa 6120 ttcgagcaat acgacgtaaa gcgcgtaatt gcaaaccttt tgcacccgtc aaagcggcag 6180 tctcataaat taatttaacg ttatttcgat taagcacacc gtggaggaga tgcacaatat 6240 aggcaaaaaa ggtttccaag ccctcaaaac ctttacaaag gatcaggaga ttacatagat 6300 ttttggcagt acaataagat ccgtttaatg tcccacaata tatgtaatgc aaaaaagcgg 6360 aacacgtttc aaatgaataa ggcatataca acgccctgct tcctgtgcta tgaatagaac 6420 tgaatggcag gtctgttaac tgaggaccca gttgagaaac ctcagcttga aaagcttcct 6480 gatttttttc aactgctcga tccataattt tttgaaatgc aggccatctc ttgtaaagca 6540 ttctcgataa acaagggatg tgcatgcgct cgattgtcaa aatttccata tcacttatac 6600 cgttcaacag tagtttaccc aactgttcag atcgctccgt aactctcccc atttgcggct 6660 ttctgtatac accatatcct tctaactcaa taaaaactat gtgatcgtaa tttgattgtc 6720 gcaaattata atcttgggta agtttcctag ttttgtttcc aaaaaccaca aacctattcg 6780 tacaatctaa gactagacat ttgaaccacg atccatggtt taaaacaccc agcatatcga 6840 gttgcaacca aagctccttg tcaagagaat agacccaaag cacgaatgcc tgtctggagc 6900 tcgtaaggta gattccagat aatattaagt tgtctccaag tatgttaaca gcaggaaacc 6960 ttagtcccgg cggcattgct gaacttaaga atttttcagt aacatcaagc tctaggaaac 7020 ggaaagattg gttattatct tttgccaaag cggctgattt tttttcactt tcggcagtat 7080 ctaattcaaa taattccaag tatattactt gcctcttaac attgacaaaa ttgtaattta 7140 aataaaaaaa caaagaaccg atagagtgct cattagaatc ctgagttgta ttaattggtc 7200 gacacatctg taaaaattca ctatctttgt ttattaccac acaattcgat ctgtatatac 7260 cacagtgatg attgaacagg gatttaaaaa cccattttcc cgaatccagg tcaaaaatat 7320 ttatctcttc aatatagctg tttgataaat cttgcccaca aaatataata agataatgat 7380 ctaacacgca atgcaaatgt ccgtaacgtg ctgatggata taagtgtttg cttttttcat 7440 caacatcaga tccattgact tctttagcat cgtcatttgt ctgattctct gttatcatgc 7500 caggtatatg agaccattgc ttagtctcaa tatcatatat gtcgatgtcg ttaagaacat 7560 aaagacattt tgtatcatca ttaaatccca taccaccaaa gaatattaat ttgttattcc 7620 ataaatcccc tgaatggaaa taccgtggta tcggcttttg atttccttga tattcaactt 7680 gcttccagga acaagtgtta agatcaacta catacatatc attaacaagc tgcgcattta 7740 atgcatcgcg tcctccatat acaaaagcct cgtctccata ttttataact gactccccaa 7800 tcagcggttt tgggacatta tttgaagata aactaaatgt agaagtaaaa atgtttgttg 7860 aatttctcaa agatatattc ctttttactt ctgacataga tataaactgt ttagcaactc 7920 gacgttaata agaaatatca aaaaagtgat ttttccttcc acataaaaat taataattac 7980 tgaaatgcaa acagtcaaaa aagatttatg caataaacaa agcagcaaat taatagccaa 8040 aaatgagaag cataaaaatc attgttgtcc ttttgtttta gtgcatattt cttcctgtat 8100 tgcactatcg tagcgttaac tgatatagtg aagttaactt ttatgcacac ctagatttta 8160 aaattcgaag cacttgaatg aataaatata aatatacact gtgataagca tattataaac 8220 ctttctctta acgtttctaa gattcatcaa tatagattcg gtaaataaat agtcttcatt 8280 gcggtactca attagattgt atttcaggta taaacacaac tggtaacgta cttacacatg 8340 gaatgataat atattaatat taaaattagc aaaactttcg tttaaaaaat attgattcca 8400 taatcccaac cttatttatt aatcattaag ccataaatac agaaatatca taaacaagta 8460 atatagtcgt gcactgggaa atgcgtttaa tagtgttcac tgcgttaatg gctgctttgg 8520 ttgccatcta ggtggaggca tatgcggttt cgaaatgggg gatttgtgct cattgatggg 8580 accctgtggt accttcaggt atttcagcag gtcttttgct agttctttaa caagcacaga 8640 atcacttgtt tggttcaaat ttgcaaccac atctggtaaa ccacaatgcg caatataagc 8700 aacactttgg ctactagctt cacacattga tttcataatt tgaaatatgt ttaggaccac 8760 aatcggcttg gtccccttat gagttaactt ttctttaaat ttctggaata ttataggctg 8820 gccataagag gctgccaatc ttggggaaat ttgacaaacc ctgcccaaag tatctagcat 8880 tctgtttgag gaagctgatt gagaaataca gaacaccttt aacactaatt gtacgtttct 8940 cttctctgcc aaaacacgtt gtacttcacg gagttcaaac tgcaaccaag atgcgatcgt 9000 atcaaatacc gcagattgcc aattaagatc tgataataac gagagaaaaa aatctaagca 9060 tttattttgc caaagataaa ttctcgacac tggaccagcc tgtggcaaag ctaataatat 9120 cggcagcgca aactccttaa acagtctgtc tttaataatc acctcttgca ataatggaat 9180 agcaccagaa ataacagcct cttcttgcga tttcttatct agcttacaca cattgaataa 9240 agcggccaaa gcttcgcttc taatttcttt tcgtcctttc tcaattttgc tgtcaccgag 9300 aatatgtgtc aaaagaggta ttatatgaac tttcctaaga agcgacaaag atgaaggaac 9360 cattgaaagt tgcttgaaaa actgtaaggt aatcgccata tcagaatgcg gcagataaag 9420 cagaatacgc aatatccttc gaagagcact ttcgcaaaga agtgattcct taacataatt 9480 atcagcctga gataaagcca ataaaatttc acatatacgt gttaatgaca ttcttgaaga 9540 atcatcgtca gtagccaaag ctttcagcat ggctttggtc aagggctcta gtgaatcatt 9600 tacaaccatc gtacagatat cattttttgg aatgtaatcc tgttgtctca gtaatttcca 9660 tattccttct acgccaacaa aaacaaagtc acggttcgtt ccatagtcct ctttaataaa 9720 taataaaagc gaattcagac catttgagct caaaaacatt tgtaatgtca aagcagaagt 9780 tctatacatc tgttgaataa agatagcact ttcatatcta aactcaaaag agtgttcacg 9840 attagagaat gacaacataa gcggtaaacc tccagcaaag caaacttttt gtaaagttgt 9900 gtgatcatcg aatgcaacct aacgttagta aaatcacgca ttaaatatcc ttacagtgtt 9960 aatgagcttt aataacagca attgaacatc aggcgttttt atttcgcgca atgtctctaa 10020 aagtggaagg ataccatgcg cttgtattat ctctcgtttt agagaaagat cttcactaag 10080 tatagaagcc tattgtcagt taaattatca atataaaaat atgcacttac tatttgcgaa 10140 acagttgtat ccttcgatcc gatattcttg tcacgtagtg agcctaacag actggaaagt 10200 aaggaagcta gatgcgttct cttatctaaa gcaatgttgt tttcaatatc caagttttct 10260 aagtttgttt caatagagtc aaagatatcc gattcttccc catcctcatt ttcttcatta 10320 ttccacgagc cataacattt gtttaaaacc aaagtcccca gtccagtctc ctttctcaat 10380 ccttgaactt tgattgagga tgtagggaaa atatcagtaa attcactatc tctgggtgtc 10440 tcgacaaatt tttcaattcc gtctggtata ttctctttaa actctagata attagttata 10500 tgttagtatc agatacttac cagttgacaa gtatctttta tgttgcggag ttttttgatg 10560 atctattgac cggttgagag cgggctaaaa aaaaggttaa ttatttattt aaaatcatta 10620 aaagctctag aaaatcgagc tgcattattt accttagagg gatcaaattg ggctttgaga 10680 ggagtactca acggtgatgc attaaagttg tttgattcag agatttgatc tgaattaaaa 10740 gcatggcgag acttcgaagg ggatgatgtt atggagctgg aattattttt tcctttaaag 10800 ttactgcaaa aatccataaa atgctcagat ttctttaaaa catcgtcact gattttaagg 10860 gttccttgaa attcattgtc ccagttatca tgatctaaaa atgttagagt agttaaaaag 10920 tatcataaag ctacctgggg actcggcaat tggtgttttt ggagactcag gcatgtgata 10980 agaagactgc ctgccagaat gaagagtcgg attgattctg ttggaagtag gctcgataat 11040 agctgtcaaa gtgctttctt tcactctttc attatatttt tgaacctcgt ctatagcgtc 11100 agaaaattta gaggaggttt ggttcattat cacccaagga tgtttaagaa gttttctggt 11160 cttaattcgt aaatttgggt ccttttggaa acattgcatt aaaaaacttt tagcagccga 11220 agaaatattt gaaggcaagg gaggatgttc atctttcacc atccgaaaaa gagcggacgt 11280 aggatctaaa tcataatagg gaggatttcc atctaacagt tcaataactg tgcagccaac 11340 actccaaata tctgaggctg ttgtggcgcc tacaagctct ataacctcgg gagccactat 11400 ataaattgtt agtaatgtta taactatacg cttagaaaag gcttactcca ataggggctt 11460 ccaacaacac tatgatcctc taaagcatta attttcgtcg caaccccgaa atctgctaac 11520 ttgatagtgc catctttagt agtaagtata ttggcaccct taatatcacg atggataaca 11580 ccctgattat gtagataaag tagcccttgt aatacttgaa acgtatataa ggccactagg 11640 ttttctggaa tctttccaaa gtttttacaa attgagcgca acgatccgtt ttcgcaatat 11700 ctatatatcg ttaacttgaa aacaattttt taaagaaaat cacgtactcc agaataatgc 11760 atagagaatc gttcgtctga taagatcccc tatatttaac aatatttgga tgctaccacg 11820 tttagtattt ggcgaccatt ataaacgaat gagtaacata cgtccaaatt tttaagcaaa 11880 tcaatctcca tctaaaaatt gttagagaaa aatagttgag atcccttcat accttaatga 11940 cggaaagatc agattttagc attttggata gtttaacttt ttttacagcc accgtttctc 12000 cattctttat atttagacca cggtatactg caccgaaggc acccttgccc aagcaatccc 12060 caagagtctg aatatgttat tgaaaatcca atggctatta acattaaata ctagcatacg 12120 atggaagatg cttggatatt gtgcatgact attgaataag ttacaaatta aaaattgaat 12180 ttatttgtaa ttttgaatga aaagacaatt tggttttgtt tacgatgtag catttgctgc 12240 gtttgaagaa gtaaggactt tatagtgtac atacacattt agtacggtac ctgaacttgc 12300 atgttataaa ctcaaaagaa gtaaatagta ataattaagc tatttataaa atcattaata 12360 aacaaagatt ctcgtataaa agaaaaagcg tattatacag acatataaaa aatattggca 12420 aggatggaac taatccgttg gttaagtaat gtcagtggaa acaccatgta cataggaaga 12480 atgagttcat tttagcttgg aaagtaaagt agaaaagaca gtgatttaaa ctacgttaaa 12540 caagtgtctg atggttatga atccgaggaa tcaaactttt ccttgtaatg ggtttagaag 12600 catcaaaatc aacttcctgt aaactcttat tttcatgatc ggtagagaag ttactgcaac 12660 aatccagttt ccgtacccat gcagtaccta aaatactatc caacgacggc cgattctggg 12720 aatcaggatt cagtaaactt agtataatct tcttagcacc ttcaggtata tatctgaggg 12780 gctgcaatgt aggtatttgt ttaatgactt cggtttggaa actggattca tcagttgaag 12840 aaataccaca agtatctgta aggattgaca tatactttga gtagtaaaga tcagttttca 12900 cggaagtttt aaatggaaag tatccagtaa acagaacttt aagcaaaata ccacatgacc 12960 acctatctgc taaaagacca caatattcta tcaattgaac tattagtacg tcgtttttga 13020 aataggatag agctcgtctt gctacacaat ttatacacat aagacttacc attttcatga 13080 aatgcttctg gtgctagata tgccaaactt ccaacagcac ccgatacgaa acgaatggat 13140 tcgttatcac caggattacc cacaatttcg ctggtaccaa aatcggtgag ctttaacaat 13200 ccatcccttc cgattaagat gttttccggc tttaaatctc ggtgcgcaat acgttgagat 13260 tgcaagtaag ctactgcttg aagaatttgc tttagataac aacatttgtc ttctaaggtc 13320 aaaacttttt cttcaatttg cttaaataaa tcgccttgag gacagtactc ttgaaccaaa 13380 caaaacccag cggatttatt aaatacgctt gtcaaagtca cacaagggca gataactttc 13440 agaatattag gatggtccaa ttttggttgg attgaccatt cccttaaaaa ataaaccatt 13500 gtattcaatc tttttttatg cgatgttttc gttttccgat atactttggc agcatagtat 13560 atctttgcgt cggtgatttt atctctgtga gttacaacct tgattgtaga ggtggcccct 13620 ttagctattg attttagatg ctgaaagtct aagcgaaatc ttatttcggg taatatacgc 13680 ttcagtgaag gatctaatcc aattgcgaag tcagacattc caataaacga aggttgaatt 13740 gaatgaacgg aaagtaagct agtattactg tctaagtatt cattttctaa ccatggtgca 13800 gtaattgtac ttggctttat aacgacggaa gacttcttgg ttgttgattt agaaacattt 13860 attgaaaaaa attggaagaa ctctttagga aatctcccaa aaatattttc aatcgaaaga 13920 ctattcttat tctctgaatt cctttgaatt gctgaattgt ccacgaaaat tgtcccctgg 13980 cgaaaaaaag gataattagt cattagtgac aagccgctga agctaagaag cctagttacg 14040 acttcaaatt gattgatttt ttatgtactt cttctttatt ttcttactaa aaaaagagtg 14100 ttttcagaac aacctcatca tttattctgt ttagataaag agttacgtgt tagcaacctc 14160 aaaattgcat agttgtaatc ataacgtctt tgaaagaaac gaaatttcca aaatcatatg 14220 ctcacgaatg cctaaatcga tgaacgaccg tttacgcatc tacgactcaa cctgaaagac 14280 gtaatgatga gcattggatt gattcgactc attactcagt aatcaacagt taaatgaatt 14340 tgtaaattaa tattttattt aaaaatacca aacgagacgt tcggcagccc tcagttaact 14400 catcgtttca agtaggtcat aacaatgcac gtgtgggtgc ggtaatctag tagtctaatg 14460 aatgcttatg ttaaggacat aggtagagaa gtaggtgtat gattaagcaa ttaaaaagaa 14520 atcaaaagac ttttcatgaa cttatactaa attattcttt tgattgttta taatttgcac 14580 tctcaaatgt ttaatttagc gatagaaagg gaggttttcg taacgctcat gagacaaatc 14640 atccattttc ttcatctcca attcctttgt cgtattgttg ggtagactgt ttcgacttga 14700 tgtagttctg tgaggaaatt cctttggcga tattacttct aatccaattt gttcacagtt 14760 taattgatta gttttagttt tagtatttcc ataagaatcg catttaagac tgctggtgtt 14820 gtcaactttg atggagttaa ttttctttaa ctcctcagat tttgtagaat cggagactgc 14880 gttcattagc acgaaaatga gagaatgcat atacttacca ttagttcgca tttctgattg 14940 atcaatcgaa gatcgagtct ccgttctctc agaaccattt acacttcttt tatcttcatt 15000 tgcaactatg gaattttgct tttgcaatga cctaacttat t 15041 //