ID SPBC19F8 standard; DNA; FUN; 16999 BP. XX AC AL023594; XX SV AL023594.2 XX DT 19-MAY-1998 (Rel. 55, Created) DT 01-FEB-2000 (Rel. 62, Last updated, Version 5) XX DE S.pombe chromosome II cosmid c19F8. XX KW 40s ribosomal protein s4 type; amino-acid permease; cdk-activating kinase; KW crk1; nuclear movement protein; nuclease; protein assembly protein; KW septin homolog; snu5; U5 small nuclear RNA. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-16999 RA Beck A., Reinhardt R., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (18-MAY-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Max Planck Institut fuer Molekulare Genetik, RL Ihnestr. 73, D-14195 Berlin, Germany. XX DR GOA; O60165; O60165. DR GOA; O60168; O60168. DR GOA; O60170; O60170. DR GOA; P87158; P87158. DR GOA; Q12126; Q12126. DR SPTREMBL; O60166; O60166. DR SPTREMBL; O60167; O60167. DR SPTREMBL; O60168; O60168. DR SPTREMBL; O60169; O60169. DR SWISS-PROT; O60165; SPN7_SCHPO. DR SWISS-PROT; O60170; ME22_SCHPO. DR SWISS-PROT; P87158; RS4A_SCHPO. DR SWISS-PROT; Q12126; CRK1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the CC Sanger Centre, are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c19F8 is overlapped at the 5' end by cosmid SPBC21, CC EM:AL035537 and at the 3' end by cosmid SPBC25H2, EM:Z95397. XX FH Key Location/Qualifiers FH FT source 1..16999 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c19F8" FT /map="IIR" FT misc_feature 1..102 FT /note="nominal overlap with cosmid SPBC21, EM:AL035537 S. FT pombe chromosome 2" FT CDS join(complement(1065..1269),complement(939..1020), FT complement(795..888),complement(132..643), FT complement(1..92)) FT /db_xref="GOA:O60165" FT /db_xref="SWISS-PROT:O60165" FT /label=SPBC19F8.01c FT /note="SPBC19F8.01c, len:327, FT SIMILARITY:Schizosaccharomyces pombe, SPN3_SCHPO, septin FT homolog spn3., (412 aa), fasta scores: opt: 763, E():0, FT (39.4% identity in 317 aa)" FT /partial FT /gene="SPBC19F8.01c" FT /product="septin homolog" FT /gene="SPBC21.08" FT /protein_id="CAA19121.1" FT /translation="MNKGPRHRPKFLSKKGKKLRIMVAGSSYTSYQACINSLCSKQILE FT AETEIDPLKAHIDRILEIREFNADILEDEFHVDLTVIEVNGFGDKIDNSASFEVVTHYL FT ESQFDQALIEESKIKRNSKFTDTRVDALLYFIAPRGHCLSEFDLEAMKRFSKRVNVIPV FT IGNSNAFTEEELKNFKDVIMKDLKQCNIKVFDFPWDPEEDEDEVIEDNKRLWESVPFAV FT SGGVSEEDEEGYQRIVKKFQWGTFVIDDPAHSDFLNLKTVLFISHLDILKSITKQTYYE FT NYRTEKLSNDSPSNTSLSLQKQNSIVANEDKRSVNGSERTETRSSID" FT misc_feature complement(93..104) FT /note="ctaataagttag, splice branch and acceptor" FT misc_feature complement(126..131) FT /note="gtaaga, splice donor sequence" FT misc_feature join(complement(1065..1209),complement(939..1020), FT complement(795..888),complement(155..643)) FT /note="Match to PF00735 GTP_CDC, Cell division protein FT Score 208.82" FT misc_feature complement(644..658) FT /note="ctaacgatattttag, splice branch and acceptor" FT misc_feature complement(789..794) FT /note="gtaaga, splice donor sequence" FT misc_feature complement(889..906) FT /note="ctaatttcaatgcattag, splice branch and acceptor" FT misc_feature complement(933..938) FT /note="gtgagt, splice donor sequence" FT misc_feature complement(1021..1034) FT /note="ctaactgtaattag, splice branch and acceptor" FT misc_feature complement(1059..1064) FT /note="gtgagt, splice donor sequence" FT CDS 2298..2798 FT /db_xref="SPTREMBL:O60166" FT /label=SPBC19F8.02 FT /note="SPBC19F8.02, len:166, SIMILARITY:Emericella FT nidulans, NUDC_EMENI, nudc, (198 aa), fasta scores: opt: FT 469, E():9e-23, (43.0% identity in 172 aa)" FT /gene="SPBC19F8.02" FT /product="putative nuclear movement protein" FT /protein_id="CAA19122.1" FT /translation="MHQVKLEEAEYEWDQTIADVDIVIHVPKGTRAKSLQVDMSNHDLK FT IQINVPERKVLLSGPLEKQINLDESTWTVEEQERLVIHLEKSNKMEWWSCVIKGHPSID FT IGSIEPENSKLSDLDEETRATVEKMMLEQSQKRTDEQKRKDVLQNFMKQHPELDFSNVR FT DQI" FT CDS join(complement(5031..5076),complement(3081..4984)) FT /db_xref="SPTREMBL:O60167" FT /label=SPBC19F8.03c FT /note="SPBC19F8.03c, len:649, SIMILARITY:Saccharomyces FT cerevisiae, YG54_YEAST, hypothetical 64.3 kd protein in FT pfk1-tds4 intergenic region., (568 aa), fasta scores: opt: FT 583, E():3.5e-21, (26.1% identity in 613 aa)" FT /gene="SPBC19F8.03c" FT /product="putative clathrin coat assembly protein" FT /protein_id="CAA19123.1" FT /translation="MSPSKWLLTYERAVKKATKVKLAAPKYKHVEIILEATTEDPETLE FT NVIQALCERLKEQSWTIVFKTLIVFHVMLKEGAPNTTIVALSQRPRILEVLKASSLLAQ FT GKNIYNYSRFLSERAKQYGRLGVDYAQVGDAPKKKIREMKLENGLLRNVEGIQAQLRRL FT IKCQFVAEEIDNDIAITAFRLLVGDLLVLFKAVNIGVINVLEHYFEMGHHDAAQSLRIY FT KTFVNQTEDIINYLSTARSLEFVTKFPVPNIKHAPISLTASLEEYLNDPDFEENRKQYL FT QNKSGSPVEETAILNRKPTLRKKKSIPKKQNESSSTIQKENTVQQEASSSEEEAVKSLP FT ETQRTTSRIETQEEEIKEEEMEGEEEEEEEEVPNYESENELEDKVGDLSLSLGVASSFV FT DEMLRERNNLSAEGTSASPSLDKKSESTNIVQPIPSHPNDSLNPFYNSSSPTYHPQPIP FT PQLQQVQLLSQMAGNQMANIQYTMNGMQQTGASPNTALNISQVNMYAQNNPVNPSTTNP FT FQNFLRQPSYQGMQFEQQQPTTIPLQPNIPVLNQQYPVMIPAMEDSRGPLPSPAHIMYP FT EGSPGFIQHSPNGFTHGHSASPVNIGQKLDVPERPMSTPYTASKNPFSTRELAEPTDLA FT RNSISTESKNPFRS" FT misc_feature complement(4656..4988) FT /note="Match to PF01417 ENTH, ENTH domain Score 147.58" FT misc_feature complement(4985..5003) FT /note="ttaacaacatttttaaaag, splice branch and acceptor" FT misc_feature complement(5025..5030) FT /note="gtaagt, splice donor sequence" FT CDS complement(5598..6290) FT /db_xref="GOA:O60168" FT /db_xref="SPTREMBL:O60168" FT /label=SPBC19F8.04c FT /note="SPBC19F8.04c, len:230, SIMILARITY:Saccharomyces FT cerevisiae, YGI5_YEAST, hypothetical 32.1 kd protein in FT mad1-scy1 intergenic region., (274 aa), fasta scores: opt: FT 304, E():2.4e-14, (33.7% identity in 273 aa); weak FT similarity to Staphylococcus aureus nuclease (SNase), FT (274aa), fasta scores, opt:304,E( ):0, (33.7% identity in FT 273 aa overlap)" FT /gene="SPBC19F8.04c" FT /product="putative nuclease" FT /protein_id="CAA19124.1" FT /translation="MQESQYKEKAIIGTGLITTSIGGFFFLRRFRRISNASTIPKNYLN FT NSTVENRKYKTMFGYVTRVGDGDNFRFYHTPGGRLLGWHWLRKVPCSRSDLSNETISVR FT LAGIDAPESAHFGKQEQPYALEAKEFLHNKLYHKSVRIIPLKIDRYARLVAGVQYYPIP FT HFFWKKDIGPQMIRKGLAVVYEGSDGVFCPTKKECLLALEIVAKKKKLSLWSQGKKLIL FT PSVYKRGV" FT misc_feature complement(5643..6017) FT /note="Match to PF00565 SNase, Staphylococcal nuclease FT homologues Score 68.63" FT snRNA 7394..7828 FT /note="snu5 gene for U5 small nuclear RNA" FT CDS 9140..9796 FT /db_xref="SPTREMBL:O60169" FT /label=SPBC19F8.05 FT /note="SPBC19F8.05, len:218aa" FT /gene="SPBC19F8.05" FT /product="hypothetical protein" FT /protein_id="CAA19125.1" FT /translation="MISRCIKRCYRSGYMIFPPNRLQSTIRSKEEINSSRHKFNIADTS FT VRKQYNEELVFTKDAKENAEAKKVSSKVKKDRKNHSAAMEGVNINVPGIYRDVYPLPNE FT DVEMHEAKKKENICKKENLSKTVLVRQLAEIYFANSNKYVVDENGLDDLIQQAFKNPND FT VFQPLPSKRDGDNMRLFIETEEEADMTSIKQHAIEVVLQDFQEEQPLEDKRDTIE" FT CDS complement(10000..11724) FT /db_xref="GOA:O60170" FT /db_xref="SWISS-PROT:O60170" FT /label=SPBC19F8.06c FT /note="SPBC19F8.06c, len:574, FT SIMILARITY:Schizosaccharomyces pombe, CAB60021, amino-acid FT permease, SPAC869.11, (537 aa), fasta scores: opt: 1622, FT E():0, (48.5% identity in 522 aa)" FT /gene="SPBC19F8.06c" FT /product="amino-acid permease" FT /protein_id="CAA19126.1" FT /translation="MLSKFYEDIDSKLENAMLSQIESSTITCDGKSLNTLPTPKQEDVE FT FGVPLQPEDTQDLQRKLKPRHMQMIAIGGCVGTGLFVGSGNALADGGPASILIAFAVIG FT TYVLFTTSALAELSAIYPVSGSFYTYFSKFIDPAWGFAVGIQYWLSFAVTVPLELTVAP FT LIINFWNASGPVSIWISVFYVIIIAINIWGTEGYGEVEFFLSIMKVISVIGFVILSIII FT AAGGVPTDDRGVIGVSYWKQPLVFNNGFKGLCAVSVIAIFSLSGTELVGLAASEAKNPQ FT KTVPAAVKQIFWRIFLFYIVALFMLTLVVPSDLPGLRTSDNSNVLISPFVIAIQLANIR FT ALPSIMNVVILLSTLSVGNSASYAASRALFALAKNGYAPKIFNKTNKRGHPIYAIAVTL FT LFGSIAYFTEAGVGGALFGWLLSICGLSTTFIWGSICLAHIQFRRAWKIQNRKLEDLPY FT RSIFGVYGSIYGVAMTILALIAQFYVAVFPIGKKPNPVDFFQAYMAAPILIISFVAWKF FT FRRTSFVRIEKIDLSEGAHGEQKQNLEPVINIPVEEIDFPMKVATKESMKNVEKNAL" FT misc_feature complement(10165..11553) FT /note="Pfam match to entry aa_permeases PF00324, Amino acid FT permeases" FT misc_feature complement(11359..11454) FT /note="PS00218 Amino acid permeases signature" FT misc_feature complement(11491..11577) FT /note="PS01067 Protein secE/sec61-gamma signature" FT CDS 14206..15213 FT /db_xref="GOA:Q12126" FT /db_xref="SWISS-PROT:Q12126" FT /label=crk1 FT /note="SPBC19F8.07, len:335aa" FT /gene="crk1" FT /product="cdk-activating kinase" FT /gene="mop1" FT /gene="mcs2" FT /gene="SPBC19F8.07" FT /protein_id="CAA19127.1" FT /translation="MDIEKSDKWTYVKERKVGEGTYAVVFLGRQKETNRRVAIKKIKVG FT QFKDGIDISALREIKFLRESRHDNVIELVDVFSTKSNLNIILEFLDSDLEMLIKDKFIV FT FQPAHIKSWMVMLLRGLHHIHSRFILHRDLKPNNLLISSDGVLKLADFGLSRDFGTPSH FT MSHQVITRWYRPPELFMGCRSYGTGVDMWSVGCIFAELMLRTPYLPGESDLDQLNVIFR FT ALGTPEPEVIKSMQQLPNYVEMKHIPPPNGGMEALFSAAGHEEIDLLKMMLDYNPYRRP FT TAQQALEHHYFSALPKPTHPSLLPRKGGEEGIKHVSSDLQRQNNFPMRANIKFV" FT misc_feature 14236..15081 FT /note="Pfam match to entry pkinase PF00069, Eukaryotic prot FT ein kinase domain" FT misc_feature 14254..14328 FT /note="PS00107 Protein kinases ATP-binding region signature FT " FT misc_feature 14590..14628 FT /note="PS00108 Serine/Threonine protein kinases active-site FT signature" FT CDS 15920..16708 FT /db_xref="GOA:P87158" FT /db_xref="SWISS-PROT:P87158" FT /label=rps4-1 FT /note="SPBC19F8.08, len:262aa" FT /gene="rps4-1" FT /gene="SPBC25H2.17c" FT /gene="SPBC19F8.08" FT /product="40s ribosomal protein S4A/S4.1" FT /protein_id="CAA19128.1" FT /translation="MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLI FT VFLRNRLKYALNGREVKAILMQRLIKVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYD FT IKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLN FT LETNKIESFIKFDTSAQVMVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRL FT SNVFVIGETGKSWISLPKGKGVKLSITEERDRRRALKGLA" FT misc_feature 15941..15985 FT /note="PS00528 Ribosomal protein S4e signature" FT misc_feature 16088..16516 FT /note="Match to PF00900 Ribosomal_S4e, Ribosomal family S4e FT Score 291.53" FT misc_feature 16370..16999 FT /note="nominal overlap with cosmid SPBC25H2, EM:Z95397 FT S.pombe chromosome 2" XX SQ Sequence 16999 BP; 5388 A; 3038 C; 3063 G; 5510 T; 0 other; gatcaatcga agatcgagtc tccgttctct cagaaccatt tacacttctt ttatcttcat 60 ttgcaactat ggaattttgc ttttgcaatg acctaactta ttagctcgct tatgcatagt 120 taatttctta caatgatgtg ttcgaaggtg agtcgttgga cagtttttct gtacgataat 180 tctcataata ggtttgttta gttatacttt tgagtatatc caaatgagaa ataaacaata 240 cggtcttcaa gttaagaaaa tcagaatgtg caggatcatc aattacgaat gttccccatt 300 ggaatttctt gacgattctt tgatatcctt cttcatcttc ctctgatacc cctccagata 360 cagcgaatgg aacactttcc caaagtcgtt tgttatcctc aatgacttcg tcttcatcct 420 cctcaggatc ccaaggaaag tcaaatacct ttatgttaca ttgtttcaaa tccttcatta 480 tcacatcttt gaagttcttt aattcttctt cagtaaaagc gttggagttt ccaatgacag 540 gaattacgtt tacgcgcttt gaaaaccgtt tcatggcttc caaatcaaac tcagacaggc 600 aatgtccacg gggtgcaata aaataaagta gggcatcaac gcgctaaaat atcgttagaa 660 aacttttttt tcattcctca ttcttaaacc aatgtgttct cttggttttc tacttaacaa 720 cattaaatca agtaatggaa gtaacttgat tgaaatagtt aaggacggaa aaactttgtt 780 tgcagatttc gtaccgtatc agtaaattta gaatttcttt tgattttaga ttcttctatc 840 aaggcttgat cgaattggct ttccaaataa tgagtaacta cctcgaaact aatgcattga 900 aattagcttt aatttagaaa aatacctttt ggtattacct agctgaatta tcaattttat 960 ctccgaaacc atttacttca atgaccgtaa ggtcaacatg gaactcatct tctaaaatat 1020 ctaattacag ttagcacacg aaaattaaaa ccgtaataac tcaccagcat taaattccct 1080 tatttccagt attctatcaa tatgagcttt tagcggatct atctctgttt cagcttccag 1140 tatttgttta ctgcacaagc tattgataca agcttggtaa ctggtgtagc tagatcctgc 1200 taccattatg cgaagctttt ttcccttttt tgataagaat tttggacgat gtcttgggcc 1260 tttattcatc ttgaagtgtt tgctgttgga agaacttggg tttgtttgtt tactgcttaa 1320 taaaggcacc gatacgttgg cagtatatcc atttcgcaaa caaggaaaat ccagaagatt 1380 tctttataca aaacttttca atagtacttt taaatatagt ttatattttt tcttccagta 1440 ttcttctcta aatcattttg tgccttaatc atcttcgcat tgtccaaaat ttaagtaatt 1500 taacctttga cctttaacga gccctctgtt catttactga gttaaaataa ggcgctgata 1560 ttttgcatta attcatgaga ctttcgagtt tatttaagcg acagcaattg ttaaaggaat 1620 cataatcatg aaaacccaat tcccatgctt attttgcatc taccacacaa attttgaatg 1680 attgcaacag ttgtaccttt cgttgctaca tataacttcg taaacataat gatgctggta 1740 gattttacat aacgatgttc gttaatgttt taatgttatg atatgcttgt tgtgatttgc 1800 gccatagccc atataaacgg ctttgagcaa atttcataat aaattgaatt gttcctaaaa 1860 ctcggtttat ttgcaaagcc taatacataa cttgagttac accttcctca aaaattgaag 1920 ttttttttta gcgcaacaaa attctacttt ttaaaaagtg aaatctttaa ctctagagta 1980 ttgtataaaa atcgaatgtt agtaaaataa acgtaacaga acacaagtaa aagttacagc 2040 ctgcgagttt gcacgactct ccactgtgtg caccgcaaac catcaaacgt tagctcttta 2100 acgtagcgta caaactcgta atcttgaaat tccggtttat gaagtcggta attaaatata 2160 cattaatcaa atctagcttc attttttagg ctcgtaaaag aatttgattt gcttagccaa 2220 aaacgagcac atatttaata aaattacaaa gtaatcaacc gattacgaaa agcgtgagtt 2280 gagctgcagg tttaagaatg catcaagtaa aactcgaaga ggccgagtac gagtgggatc 2340 aaacaatagc cgatgttgat attgtcatcc atgtacccaa agggacaaga gcgaaatccc 2400 tccaggtaga tatgtcaaat cacgatctca aaattcaaat caacgtccca gaacgaaaag 2460 tccttctcag tggtccactc gaaaaacaga tcaatttaga tgaaagcacc tggactgtcg 2520 aagaacaaga aaggcttgtt atccatctcg agaaatccaa taaaatggaa tggtggtcgt 2580 gtgttataaa agggcatccc tcaatcgata taggctcaat tgagcccgaa aattcaaaat 2640 tatctgatct agatgaagaa acaagagcta cagttgaaaa aatgatgttg gagcaaagcc 2700 aaaagcgtac cgatgaacaa aaacgaaaag atgtactgca aaatttcatg aaacaacatc 2760 ctgaactaga tttttcaaat gttagagacc agatttgaat agtttcatga ttttatatat 2820 gtataattac aattgttaca aaactttcta agctctattg ataaaaatgt agaaatttct 2880 ggtatttgta aaactgatag tacaaagaag gtataaacag caaatcggca ttcttttaaa 2940 ctagaaataa atcgagaaag tcttggataa agagcgagtt agtataataa tattaataaa 3000 tgggtcatta gctagccagc aataacttat gcatgattat atacaacgac atgatgggtg 3060 cattgacaac aaaaacaaac ctaacttcgg aacggatttt tagactccgt cgaaattgaa 3120 tttctcgcta aatcagttgg ttcagccaat tccctcgttg aaaaggggtt tttagatgcg 3180 gtgtacggtg tagacatcgg cctttcagga acatccaact tttgtccaat attaactggg 3240 gaagccgaat ggccatgcgt gaacccatta ggagaatgtt gaataaatcc aggagaccct 3300 tcaggataca taatgtgagc aggggacggt agcggacccc ttgagtcttc catggcagga 3360 atcataacag gatactgctg attcaataca ggaatatttg gctgtaacgg tatggtagtc 3420 ggttgttgct gctcaaactg cattccttga taagagggtt gccttaaaaa gttttgaaaa 3480 ggattggttg ttgaaggatt tacaggatta ttttgagcat acatgttaac ttgggaaata 3540 ttcaaagcag tgtttggaga agctccagtt tgttgcatcc cattcattgt atattgtatg 3600 tttgccatct gatttccagc catctgggac aagagttgaa cttgctgtaa ttgagggggg 3660 attggttgtg ggtgataagt tggtgaagag ctgttgtaaa aagggttgag agagtcattc 3720 gggtgactcg gaataggctg cactatatta gtactttcag attttttatc taatgaaggt 3780 gaagcactcg ttccttcagc gctcaaattg tttcgctccc taagcatttc atcgacgaat 3840 gaagaagcaa cgcctaaaga aagagagaga tcgccaacct tgtcttcaag ctcgttttcc 3900 gattcataat ttggtacttc ttcttcctcc tcttcctctt ctccttccat ctcttcttct 3960 tttatttctt cttcttgtgt ttctatacgg ctagtggttc tctgggtttc cggtaaactt 4020 tttacagctt cctcttcaga agacgaagct tcttgttgaa cagtgttttc tttctgtata 4080 gttgaagaac tttcattttg ctttttgggg atgcttttct tctttctcaa agttggcttt 4140 ctatttagaa tagcggtttc ttcaacaggc gatcctgact tattctgaag atactgttta 4200 cgattttctt caaaatccgg atcattgagg tattcttcca aagatgcagt taagcttata 4260 ggtgcatgtt taatatttgg aacaggaaac tttgtaacaa attctaaaga tcgtgccgta 4320 ctcagataat tgatgatatc ctcagtttga ttaacgaatg ttttgtaaat gcgtagagat 4380 tgagctgcat catgatgacc catttcaaaa taatgttcca aaacattaat aacgcctatg 4440 ttaaccgctt taaaaaggac aagtaaatct cctactagta gacgaaaagc agtgatggca 4500 atatcattgt caatctcctc agcaacaaac tgacatttga tcaatctcct caactgagct 4560 tgtattccct ctacattcct taaaagacca ttttcaagtt tcatttcacg aatttttttt 4620 ttgggagcat cacccacttg agcataatct actccgagac ggccatattg tttcgctcgt 4680 tcacttagaa atctgctata gttgtaaatg ttttttcctt gtgccaataa tgatgaggcc 4740 ttgagtactt ctaaaattct tggtctttgc gataaggcaa ctatagttgt attcggagcc 4800 ccttctttga gcatgacatg gaaaacgatg agagttttaa agacgatagt ccatgattgt 4860 tcttttagtc gttcacacag tgcttgaatt acattttcca aagtctctgg atcttctgtt 4920 gtagcttcca aaattatctc aacatgctta tattttggtg cagcgagttt aacctttgta 4980 gcctctttta aaaatgttgt taatatggag ataaaaaaga attaacttac ttttaacagc 5040 ccgttcataa gtcaataacc actttgatgg agacattatt ggtttatggg gtgtaggtga 5100 gcagttctat caagaaccac agcgaatgta actcaattcc aaaaacaagt aaaatttaaa 5160 cgcaaaaaat cggtatttgg gatataatta acaattacac cgtttcaatt tataaccagg 5220 gagcgggtta acgagaacaa gtacaagagt agggcgcagc ttgattgtat taacgagatc 5280 catagaactt aatgatgaat tttgctatat acaagtatta aatttgctga tgtaatttat 5340 atttaaagta atgataatag taggatttgg aaatttatat accttttacg aaagacaata 5400 atgtatcaaa atcttattat agaaaaagat tcacccatgt attaatcaga aaagtcatta 5460 aaaaaaattt gcagatcaaa aaggaattta aactgtaaaa ctagggacat aatcacgaaa 5520 aaaatcatta aaagtagcaa acgactgaag aaaaaaaaat ttgaaagatt ggaattcaac 5580 ttcctagcat aacaaactta aacacctctc ttgtacacag acggtaaaat cagcttttta 5640 ccctgagacc aaaggctcaa ctttttcttt ttggcaacta tttctaaagc taaaagacat 5700 tcttttttag ttggacaaaa tacaccatca ctgccctcat aaaccacagc caaaccttta 5760 cgaatcatct ggggaccaat gtctttcttc caaaagaaat ggggaattgg ataatactgg 5820 actcctgcaa caagtctagc gtagcggtca atttttaggg gaatgatcct aacgctttta 5880 tgataaagtt tattatgcaa gaactctttg gcttctaagg cataaggttg ctcctgttta 5940 ccaaaatgcg cagattctgg ggcatcaatt cctgcgagac gcacagaaat ggtttcattc 6000 gataaatcag atctactgca tggcactttt cttaaccagt gccatcctaa caaacgacct 6060 cccggagtat gataaaaacg aaagttgtca ccgtcaccta ctctagttac atatccgaac 6120 atagttttgt atttccgatt ttcgactgtc gagttgttaa gataattctt gggaatagta 6180 ctagcattag atattctacg aaaccgtctt agaaaaaaga aaccaccaat tgaggtagta 6240 atgagaccag ttccgattat ggctttttcc ttgtattgag actcttgcat gattgtatgt 6300 tatcactttt cacaacttta tttcctcaat ctgtatcgca tttgcatcca aatgctagga 6360 ttttgaaaat tagagataat tcttcaaaaa accttaccta gaaagatgga atgaattgtg 6420 gaaggtataa cacataaaaa aactattccg ttttaaaagc atccaaagaa gtttagtatc 6480 atgaaagaaa ttcaactagt gtagttcaaa tttgggtggg agcggttttc gaaaaggaca 6540 gagaagtaat ttacgttatt aaaaattaaa ttcaagcaat tagtttattt actaattaag 6600 atagcactaa gtgataataa agttaatttg tgaataattc gacgcttgta aattaaacca 6660 atttgtcatt ttaatcgaaa tgcctaagat tatacagcaa ggcaaaaaat tccttattgt 6720 aagaaatcag cttttttcag ctaagtcatt taatatcaaa tattttaact aagtccgtca 6780 gctttgtaaa cagtatcaaa catgcaaatt ggatttgcgt ttctcagtac tcgctaggtt 6840 tattttctta tctccattga gtttgtaatt aggagaattt actaacctcg acaataaaag 6900 ctaaactaat ctaaattgaa atttttttta attctgaaaa gtattatttc aaatgaggac 6960 aaaaatttgt tatttccgaa ctacactact tcggttctta atcattacaa aatcgagtat 7020 tcataaattt tgcttaacgt tgtaaatata tcctgcagat ctgtaaacac atctataaaa 7080 taataacaat atatatatat attataggta tggttgattt actcttggta gtatctaaat 7140 tttcacaaca tcgatttctg tatcgttttc gtttgtttca tcataaattt tagaagtcgc 7200 agatccctta taagttaatt taacaaatgt aaccaacaca aatactaatt tcattcatct 7260 ccctggtcca aacgatttaa gtaaaaatta atcaatcggt tcattctgta gcacacattt 7320 gctagtaaaa tgtgcattgt tttcttttaa tcattttaaa tgtttatcga atagtgagct 7380 ttaagataat gaatacatca tttgcaactc ttctgttgct gaacatgttt cggttcaact 7440 caccaaactt cattatccaa tgtgtagatt aaataattta caagcaatta ttatttataa 7500 aataaaatat tacatttatt ttaaagagat aaaatagtta tttatgtaaa actcctaaaa 7560 tttgctttct tagattttgt acagggttag ggtttgaggt tgcaaaaagt tatataaaca 7620 cctggttgca cacacatttc agcataatcc gtcaaagcac tttgcaaaag ctaacgtatc 7680 tgtttcttgc cttttaccag aaacagccgt ttgtaaggtg tgctaatttg actgtatagt 7740 ttttgtaatc tttttcttga aacatttatt tgtttcgggg gattttcacc aacattagga 7800 caaagttcta aggtaggtaa aaatttttcg ataacaagtg ttttgtgttt ataattcgtt 7860 gtgtaaagta atctactctc cttttggaat gagacgtatt caataatgaa cttgcgtcta 7920 tatagtatat tgagcattgt aacaaatcct aacatgcttc aagtgttgcc gtccgttttg 7980 atcttagcat gtttggtaat gacgatggat gttataaggc ccgggtggtt gatttccaaa 8040 tattatatgt tgaaaatgtg taatgttaca gcgagaagag gattttgtga cctacaatgt 8100 catatgagtc taggtccaac tttacgaagg tgataattgt accgaacgat tgggttatat 8160 gtaccatctt cactattccg ttcccaagtt tgtaggataa aaagcaatgt tgcaactatt 8220 ttaactatat caacttgatg tattatcaat tttaacagct tgaaacattt tttaaaaatg 8280 catttctagt tcaaaaagaa atagttttag atcaatttac aaaaaagtat aataataata 8340 aacaaaataa aaatttaata attattaaaa atcttaatcc attgctttaa ttaaaaataa 8400 tttttggaat attttactaa agtaccagta gtctgtatct gaacttttaa tatttattcc 8460 ttttgtttac tcattgaaaa cttttttttt cttttttatt ttaaagtaaa aaagactgac 8520 tagagtcatg gttccaccat tgaaacaatt gaacagtgtt agtatttttt gtaaaatggc 8580 ttataattgt ttttgctata cattactgac tttgcaatgt ttaactgcaa tttaacattt 8640 atctcggagc tattattata tttataagct agcgcaaaaa atttaaaagg ttagtttggt 8700 tacgaattat caataaattg aatatatttg tttgcgcatt tttgttgctt cagagatatt 8760 tgctttgcaa ttttcttagt aaattgcact acaataaaaa ttatatcgaa tgaatttctt 8820 tacaatgaag agcaaatcga tgctcaacgt ttagcgcatt ttcaacaatg gtcaatcact 8880 ataattaatt cattggtgct ggcgtataca tcctccacct tcttaccatc cctccacatc 8940 tctcgttagc tattcaagca ggtagttcat ttcgcctgcg tagaaagagt catttaatcg 9000 tcagttcatt aacgattttg gctttttagt tcataaaact ctaagagaaa ttcgtttttg 9060 attcatttcc cctgaaactt cgatttagaa acattaggct ttacctgtac ttgcttctcc 9120 acgatccaat ttgtttaata tgatttcgag atgcataaaa aggtgttata gatcaggata 9180 tatgattttt cctccaaacc gtctgcaatc aacgatacga agtaaagagg agattaattc 9240 gtcgcgtcac aaatttaata ttgcagatac ctccgtccga aaacaatata atgaggaact 9300 tgtctttacg aaagatgcca aggaaaacgc agaagctaaa aaggtctcaa gtaaagtaaa 9360 gaaagatagg aaaaatcatt ctgccgccat ggaaggagta aacattaacg ttcccggaat 9420 atatcgggat gtatatcctt tacccaacga agacgttgaa atgcatgaag ctaaaaagaa 9480 agagaatatt tgtaaaaagg aaaatttgtc taaaacagtt ctggtccgtc aactagccga 9540 aatatacttt gcaaatagca ataaatatgt cgtcgatgaa aacggtcttg atgatctcat 9600 tcagcaagct ttcaaaaatc caaatgatgt tttccaaccg cttccttcca aaagagatgg 9660 tgataatatg agactgttta tagaaactga agaagaagct gatatgacta gcattaagca 9720 acatgcaatt gaggtagtcc tccaagattt tcaagaagaa cagcctttag aagataaaag 9780 ggatacaatt gaatgatatc ttacacacaa aagctaacct caaaaatttt tatttaatta 9840 attaaaactg tatgcatcaa gaaacccaat tttgggtttc cgcaaaaagg aaatgtcaat 9900 atctaataat tcttctatgg ttcattcaat aaacaactat caaataagtc attctttgat 9960 aaagataact tcggactttg actctgcaag cgaattcaat cataacgcat ttttctctac 10020 atttttcatg ctctccttgg tggctacttt cattgggaag tctatctctt caacgggtat 10080 attgatgacg ggttcgagat tttgtttctg ttcgccatgc gcaccctctg aaagatcaat 10140 tttttcgata cgaacaaatg atgtacgtct aaaaaacttc catgccacaa agctgataat 10200 taatatgggt gcagccatat atgcctgaaa aaaatccact ggatttggtt tttttccaat 10260 tgggaatacc gcgacataaa attgggcaat taaagccaat attgtcatgg ccacaccata 10320 aatagatcca tatactccaa aaatgctacg gtaaggaaga tcttctagtt tcctattttg 10380 tattttccac gctcttctaa attgaatatg ggcgaggcaa atacttcccc atataaatgt 10440 tgttgacaaa ccacatattg ataacaacca accaaagagt gcgccgccaa ctccagcttc 10500 agtgaagtat gctatagaac caaataaaag cgttacggca attgcataaa ttggatgacc 10560 tcgtttgtta gttttattaa aaatcttagg tgcatagccg ttctttgcta aagcaaacaa 10620 ggcacgtgac gccgcatagg aagctgaatt tcctacggat aatgtggaaa gcagaattac 10680 tacgttcatg atgctcggaa gtgcccttat atttgccagt tgaatggcaa taacgaatgg 10740 tgaaattagc acatttgagt tatcgctcgt gcgtagccca ggtaggtcag acggaaccac 10800 caaagtaagc atgaaaagag caacaatata gaacaagaaa atacgccaaa atatttgttt 10860 gactgctgcg ggaactgttt tttgaggatt ctttgcttct gatgcggcaa gaccaactag 10920 ttcggttcct gacagggaga aaatagctat aacagaaaca gcgcataacc ctttgaatcc 10980 attgttaaaa accaacggtt gtttccaata tgacactccg attactcctc gatcatcagt 11040 tggtactcca cctgctgcaa ttattatgct tagaattaca aaaccgatga ctgaaatgac 11100 tttcatgata ctcaaaaaaa attccacctc accatacccc tcggttcccc aaatgttaat 11160 cgcgataata attacgtaaa aaactgaaat ccaaatcgaa accggaccag atgcattcca 11220 aaaattaata atcaacgggg caactgtaag ttctaatgga acggtcacgg caaaagataa 11280 ccaatactgt ataccaacag caaaccccca tgccgggtcg atgaatttcg aaaagtatgt 11340 ataaaatgaa ccggaaacgg gataaattgc ggagagttct gccaacgcgc tggttgtaaa 11400 taaaacataa gttcctatca ctgcgaaggc tataagaata ctagcaggac caccatcggc 11460 cagtgcatta cccgaaccaa caaacagacc agtgccaacg catcctccta tggcaatcat 11520 ttgcatatgc cggggcttca gctttctttg gagatcttga gtgtcttctg gctgcagagg 11580 aacgccaaac tcgacatcct cttgtttagg ggtaggcaat gtgtttaggg atttaccatc 11640 acaagttatt gtactgcttt ctatttgaga taacattgca ttttccaatt tggaatctat 11700 gtcttcgtaa aatttggaga gcataactgc tttgtgaatg tgaaacgtgt ttgatgatta 11760 ccacttgctt gtcggcttgt agaatagtaa tctaaagttt taaaagagga aagacgtcaa 11820 aaagggatta ccaagatatt ggaaataata atgataataa tttatatcca ggcgaatgtt 11880 attaaataaa ggtactgcaa aaggactatt agaagcgaaa aaagagtgct ggttattgaa 11940 gctgagccgt gatggatttg caaagaacac ttattgtttg taaaaccaag tttggtgcag 12000 tgaaaacaga atatagtttt gggaataaac taaacacttt gtttgtaagg ccggcatttt 12060 ggggcatggt tcgttctttt gtaggaaaat gccaggcatt ctctcaatat gatggaattg 12120 aggatatggt atgtaaatac agatttgaca tcaataaatc ctgttgttca gtcatcttga 12180 cttatattaa gggactgttt gtggggtaga atgactgtgt gatttcgtca aagtatagtt 12240 gaaatgtgga gaagcttaat ggagatggtt caatcacttg agagatgcat aaataagagt 12300 ctaagccgta atttaaaatt agtaagtaag agaaggaaca aggtcggaaa ttcgtttatc 12360 tgtgagctta tttctgtatc aaagcatagt aatcagaagg tcatctgaaa aaggggaggg 12420 gggttattaa aaaatttaat catattggaa ctaaaagaat aaagagaaga gacattgaag 12480 tgagagacga agtgaatcgt aaagagcgat ctcagtttcc agtcatggat ttgtttgttt 12540 acaacaactc caattatttt gtatgcctct catgatacaa taaaagtgtt tttaactgga 12600 atgagaaaat attaaaaata attattatat caattttttt tgttacacag tccacattca 12660 ccgtacaaca aaacaaatcc cccaacgact attgcttttt ctcttttcat atatacctgt 12720 gtacccccat cacccatcgc cttttgcaat ggaggatgtg gagctgtgtg tatttggcaa 12780 taacgaatac tacccttgta aacggtatat tcattaatat aatccatcgc atcagtattt 12840 ggatttgtag tttcgtaata ccgtcattaa ctagctagtt actatgtgct ccctagtcta 12900 taataacaat tcttgacatc gtaagcattt tgattaattg attgtcttgt agctagtttc 12960 cttcacattt tgacatagag agcgcgaagg gctccgttaa atgagttccc aacaaaatga 13020 atcaaaggca gtgcgagttt tcgtggggtt tattggaatc tgtcaattaa aaataaaata 13080 aaataataga ataacaacag gaaggaaaaa agctagcatg tcatttgtta agtgtgagat 13140 tggtagtaaa ttggagaact gcttataaac agtctttccg gttcgctagt aatggtgtcc 13200 aaagggagtg taaattaaaa gggtggataa gtctcatctc agcatggaac ctaaataaat 13260 tattgtttag caggcagaat tgttatgagg gaagaagtgg agggaaaaca attttcaaat 13320 ggcatcgcga tataattaga gtaaattgtg gcacaacgaa tagagtgtga tgcgattcac 13380 caaccgtggt gttaaccatt aaagcccctg tttttcaaga aaactacctc ttttacttca 13440 acatgtactg cctgtgtgcc tttgtatctg tcatatctac tcaacgacta ttagtattac 13500 tcagagctat ttacttcttc cttttttctc caacgtagat tgaatttcaa taaaatcgaa 13560 ttacgaaagt gaacgtgctg caagagttta ctctacacct cgttatgtaa caagcattca 13620 agataagcca cagagcccct tacactgact ccacacacga tgctggtcga taaaccccag 13680 actaatcgaa tcttaaaatt taccacaaaa gtgacatgct acccatccct tagtagtcac 13740 tgtaattatt gctacaccaa taatgaacca aatttgtggg tcctttcgac cattcctgct 13800 ttgttgagtc caagtcgatg aattagcgtt ttacaagatt acgaaagcca tgcggtaacg 13860 tttgctaaac cgagaatcct gaatacatat attggtgaat ttcttccatc ttaatttaaa 13920 acgtgggtgt ttgcttacaa acgttgggag tagtataaag atcaaaagat tgcgttgcga 13980 agtagtgtgt taagtaccta cttcatttat atacacatcg agaaagctct tgtaaaaagg 14040 ttttccctat aagaaaaagt cttgctggaa ttgtttgtgt gtttttgttg tatatagctt 14100 cactaccata ccatcatact gctatacaaa cctgtcctca atttacttac cactgtattc 14160 taaagtactc tgtaattaat agtatagggg ttaaggtttc tcgttatgga tatcgaaaag 14220 tctgacaagt ggacgtatgt gaaggagcgc aaagttggtg aaggaacgta tgccgtcgtg 14280 ttcctgggtc gccaaaaaga aactaatcgg cgtgtggcta ttaaaaagat taaagtgggc 14340 cagttcaaag atggtatcga tataagtgct ttacgagaga tcaaatttct tcgggagtca 14400 aggcacgaca atgtcatcga gttagttgat gtattctcca caaagtccaa cctcaacatc 14460 atcttggaat tcctggacag tgatttggaa atgctgatca aagacaaatt catcgttttt 14520 caacctgccc acatcaaatc gtggatggtc atgttgttac gcggtttgca tcacattcat 14580 tctcgcttca tccttcatcg agatttgaag cctaataacc ttttaatttc gagtgacggt 14640 gtcctaaaat tggctgattt tggcctttct cgtgattttg gcaccccaag ccacatgagt 14700 catcaggtca ttacacgatg gtacaggcct ccagagctat ttatgggttg tcgaagctat 14760 ggcaccggtg ttgatatgtg gagtgtaggc tgcatttttg cagagttgat gcttaggacc 14820 ccgtatttgc caggcgaatc tgatctggat cagcttaacg taattttccg cgctcttgga 14880 actcctgaac cagaggtcat taaaagcatg caacagcttc ccaactacgt tgagatgaaa 14940 cacattcctc ctcctaacgg tggtatggag gctctttttt cggcagctgg ccatgaagaa 15000 attgatttac tcaagatgat gttggattac aatccatatc gtcgtcccac tgctcaacaa 15060 gcccttgagc atcattattt ttctgccctt cctaaaccca cacatccttc tcttctccca 15120 cgcaaagggg gtgaggaagg aatcaagcac gtctctagtg acctgcagcg acaaaacaac 15180 tttccaatgc gtgcaaatat taaatttgtt taatgattga tcgtgtgcta gtatgcacct 15240 ttaactatat accattcttt tctcattcat gtttccctac agaaaaattg gaatgatgtg 15300 actgtatgcg gatttttctg tttagataat tgtcattcta ttttttagac gtttactcta 15360 ctcgctgata atttcgatat caacgttata cgaaaatatc ttctacttag cttcatttcc 15420 ttgcggtact ctttgaggta ttatcgccca ttgcttaaga gttaatggaa attgattgct 15480 ttttctaacc atatttcaga attatggaca ctcttatttt catctgataa tacaatactc 15540 attccaacgt ttaatgattt atgtaaacat gcaatccatg tttttaacat atttggtagt 15600 aaagctagaa ttatgatgta tttataagat cgttaaatct cttatatata agttttatac 15660 aatatgaatt aatcaataga ctagcgaaag ccttcttgta aatcgtactc cagcttttct 15720 ggaaaactgg tacgtccaaa aactcattaa attattaacg tggtacgtta cggtttctaa 15780 tttaattatt aaacgagcga gtactttgac agttaaatag gctactgtgg tgcactcggc 15840 atcgatattc tatacaaagc agtcacatct tttgacactt ggccaaaatt ttggatattg 15900 aatactgcat ccaccaaaaa tggttcgagg tcctaaaaag catttgaagc gtgttgctgc 15960 ccctcaccac tggcttttgg acaagctctc cggaacttac gctccaaagc cttctccagg 16020 tcctcataag gcccgtgagt gccttccttt gattgttttc ttgcgtaacc gtcttaaata 16080 tgctttgaac ggacgtgagg ttaaggctat ccttatgcaa cgtctcatta aggtagatgg 16140 aaaggttcgt accgactcta ctttccctac cggctttatg gatgtgatct ccgttgaaaa 16200 gactggtgag cacttccgcc ttgtctatga cattaagggc cgtttcactg tccaccgtat 16260 tactgctgaa gaggctaaat acaagctttg caaggtcaag cgtgttcaat taggtgctaa 16320 gggtgttcct tttctcgtca cccatgatgg acgtactatt cgttatcccg atccattgat 16380 caaggtcaac gacaccataa aacttaactt ggaaaccaac aagatcgagt cgttcattaa 16440 atttgatact tccgctcaag ttatggttac cggtggtcgt aacatgggac gtgttggtac 16500 cattgtccat cgtgagcacc atcttggttc ctttgaaatc attcatgtta aggatgcttt 16560 ggaccgtgag tttgctactc gtctttccaa cgtttttgtc attggcgaga ctggcaagag 16620 ttggatttct ttgcccaagg gtaagggtgt caagctcagc attaccgaag agcgtgaccg 16680 cagacgtgct ctcaagggac ttgcctaaat attatttttt caaaaagcaa atgcacattt 16740 tgttataata tgatggatgc gttttcgtaa ttttgtgttg attttaagac atgtaacttt 16800 aaactcattt tagatatgtc gcgtgcataa gtaatagtag cattaataat tcttgtaaat 16860 cgttgtgttg tgtgacctgt ctttttaaaa attcaatgat gatcttcgat aatactttcg 16920 aattcaccag ttggagaggt ttgttagcta aagaatgtga ataatttctt agttggatat 16980 gcgtgttggt aaaatactc 16999 //