ID SPBC182 standard; DNA; FUN; 3149 BP. XX AC AL035583; XX SV AL035583.1 XX DT 01-MAR-1999 (Rel. 59, Created) DT 16-MAR-1999 (Rel. 59, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c1826. XX KW helicase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-3149 RA Saunders D., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (28-FEB-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O43065; O43065. DR SPTREMBL; O43065; O43065. XX CC Notes: XX CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. XX CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. XX CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). XX CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. XX CC The length in codons is given for each CDS. XX CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. XX CC Cosmid c1826 is overlapped by cosmid c25B2, EMBL entry SPBC25B2, CC accession number AL031853 at the 5' end and by cosmid c6B1, CC EMBL entry SPBC6B1, accession number AL021838 at the 3' end. XX FH Key Location/Qualifiers FH FT source 1..3149 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1826" FT /map="IIR" FT misc_feature 1..101 FT /note="nominal overlap with SPBC25B2 S. pombe chromosome 2" FT CDS complement(1..3149) FT /codon_start=3 FT /db_xref="GOA:O43065" FT /db_xref="SPTREMBL:O43065" FT /note="SPBC1826.01c, len: >1049 aa, SIMILARITY: S. FT cerevisiae MOT1_YEAST, PROBABLE HELICASE MOT1., (1867 FT aa)fasta scores, opt:1544, E ():0, (33.8% identity in 1072 FT aa overlap),overlaps and exte nds SPBC25B2.12.c & FT SPBC6B1.01c" FT /partial FT /gene="SPBC1826.01c" FT /gene="SPBC25B2.12.c" FT /gene="SPBC6B1.01c" FT /product="putative helicase" FT /protein_id="CAB37625.2" FT /translation="DVAPTLVHQQNSTSSADKKTGADYNFTAQSRSDRLVVEHKAPIVP FT SAAVAVTSDSVWPFETLVELLLIDMFDPSWEIRHGACMGLREIIRYAGFGYGRVVGKSE FT AENEQLNKKYFDDLLCRIACVFALDRFGDYLADQVVAPIRESVSQVLGVALIYVPNDSV FT FSMYKVLHSLVFQNELGLTNTVWEAAHGGMLGIKYLVAVKYPLFFSHSDYLDSLINTVI FT HGLANHDDDVRAVSALTLLPIADKLVQEKLSSCKNLLKVLWDCLDDVKDDLSSSTSCVM FT DLLSSLCSFTEVMNLMQETANSDPEFSFETLVPRLFHLMRYTLTGVRRSVVYALTKFIS FT VQTSCSWITGLTLRLCFQNVLLEQQEDISKSSCELAQRVMDILYRDGPESFSKLLYSHI FT EPMLKVSITPIGSFRRPYPLDTTLIVKPSGQPYAPSTSRERNNNISELSNSRTKHRAKD FT DPKGSFCFSVDEPMLNGDVEFVGEERMLKARLRASSLLGRIIGRWKRDEILLFFKPFLQ FT ACLTSSFSTPVVLGSRLIESFFEVEDNDLTIQKDELYHLLCDQFATVPRENYANLVSQL FT HVVRAQCNALLNTFLDVGRLSRSKIPSLAVVVKGDPEAGPIAFGIADAEKLVGPTYENL FT CKLLSPSQKAQSSKALNEIKYLIIDEISIYKIAKERQDIQCSASIASAMVTYDKLPKKL FT NSIIKGIMESIKKEQFSCLQMHSASAMMKLISACYKESRQVISEKIVRNLCAYVCMDTT FT ETPIFHDSGKNGILSLHSIGTSDDNDEQVSGKLVDDSDDVSNDRKSSLSSVSDKDAAVL FT QRMGAQLTLQQMAQNFGSSLFSRVPVLSQCLFVPLQQYAESGFPSEVDQASCTVGQDLL FT DAMSILRFLVAYLDSGLQSEIVSTLPHLLATLQSNYSAVRNMASKCFAAITESNAAGSK FT ALHLLVEDVVPLLGDASSTIHRQGAIECIYHVVQRLGVRILPYILYLIIPLLGRMSDAD FT QDVRVLATTSFATLVKLVPLEAGLPDPPDLPQYLLDSREKERKFLEQMLNPSKVEAFSI FT P" FT misc_feature 3049..3149 FT /note="nominal overlap with SPBC6B1 AL021838 S.pombe chromo FT some II cosmid c6B1" XX SQ Sequence 3149 BP; 1049 A; 634 C; 608 G; 858 T; 0 other; aggaattgag aatgcttcga ccttagaagg attcagcatt tgttctaaaa atttgcgctc 60 cttttcacga ctatcaagta gatattgagg caagtctggc ggatcaggaa gtccagcttc 120 aagtggtact aatttaacaa gtgttgcaaa ggaagtcgta gctagcacac gtacatcctg 180 gtcagcatca ctcattcgac ctaacaaggg aataattaag taaagtatgt agggaaggat 240 acggactcct agacgttgaa caacatgata tatgcactca atagctcctt gtctgtggat 300 tgtgctacta gcatctccta aaagtggaac aacatcctca acaagaagat gaagagcctt 360 tgatcccgca gcatttgatt cagtaatagc agcaaagcat tttgatgcca tattgcgtac 420 ggcggaataa ttcgattgta aagttgctag taaatgaggc aaagtactga caatttcaga 480 ttgaagaccg gaatctaagt acgcaactaa aaatcgcaat atagacattg catccagtaa 540 gtcttgccca actgtacagc tggcctggtc aacttcacta ggaaatccag attcagcgta 600 ttgctgtaaa ggaacaaata gacactggga caacacgggt accctagaaa atagggagct 660 gccaaaattt tgagccattt gttgaagcgt tagctgcgca cccattcttt gtaagacagc 720 agcatcctta tccgaaactg aagacagcga agactttctg tcgttactta catcatcgga 780 atcatcaact aacttccctg agacctgttc atcgttgtca tcgcttgtgc ctatactatg 840 taaagacaat attccatttt tccctgagtc atgaaatatt ggagtttcag tagtatccat 900 acatacataa gcacataaat ttctaacgat tttctcagaa ataacttgcc gagattcttt 960 atagcatgct gaaattaact tcatcatagc tgatgctgaa tgcatttgaa gacaagaaaa 1020 ttgttccttt tttatggact ccataatacc cttaattatt gaatttaact ttttcggaag 1080 cttatcatat gtaaccatcg cggaagcaat tgaagcacta cactgaatat cctgtctttc 1140 ttttgcaatt ttgtaaatag agatctcgtc aattattaaa tattttatct cgtttagggc 1200 ttttgacgat tgcgcttttt gagaggggga caacaacttg cataaatttt catatgtagg 1260 accaacgagt ttttcagcat ccgctatacc aaaggcaatt gggccagctt cgggatcccc 1320 tttgacaaca acagctaatg aaggaatttt tgaacgagaa agacgcccta catccaaaaa 1380 ggtgttgagc aatgcattac actgggcacg tacaacatgt aattgtgaaa ctaagtttgc 1440 gtaattttct ctaggaacag tagcaaattg gtcacaaagc aagtgataca attcatcttt 1500 ttggatagta aggtcattat cttcaacttc aaaaaaagat tcaatgagtc tagaacctaa 1560 tacgacaggg gttgaaaaag acgatgttaa acaagcttgt aagaatggct tgaaaaacaa 1620 aagaatttca tctcttttcc aacgtcctat aatcctacca agtaaagagg aggctctaag 1680 ccttgccttt aacattcgct cttcacctac gaattcaacg tctccgttaa gcataggttc 1740 gtcaaccgaa aaacaaaaag acccttttgg atcatctttt gccctatgct tggtgcggct 1800 attacttaac tcggaaatat tattattgcg ttcgcgagac gtagaaggag catacggttg 1860 accagaaggt ttgacaatca aggtagtatc caaaggatat ggcctcctaa aagaaccaat 1920 gggagtgatt gagaccttta acataggctc aatatgagaa tataataatt tagagaaaga 1980 ttccggtcca tcacgataca gaatgtccat aacacgctga gcaagttcgc aagaggattt 2040 cgagatatcc tcttgttgtt cgagtaaaac attttggaaa cataaacgaa gagttaaacc 2100 cgtaatccaa ctacatgatg tttgtacgga aataaatttt gtcaaagcgt atacaacaga 2160 tcgacggaca ccagtaaggg tatagcgcat aagatgaaaa agcctaggaa ccaaagtttc 2220 aaatgaaaac tcaggatcgc tatttgcggt ctcttgcata aggttcatta cttcagtaaa 2280 cgagcataaa gatgacagaa ggtccataac acaagaagta gaagaagaaa gatcatcctt 2340 aacatcgtct aagcaatccc acaaaacttt tagcaaattc ttgcaggacg ataatttctc 2400 ctggaccaat ttatcggcga ttggcaagag agtaagtgca ctaacagcac ggacatcatc 2460 atcgtgattt gccaagccat gtattacggt atttattaaa ctgtcaaggt aatctgaatg 2520 ggaaaagaat aaagggtatt taactgcaac aagatatttg ataccaagca taccgccatg 2580 agcagcttcc cacacggtat tcgtaagacc tagctcattt tgaaaaacaa gtgagtgtaa 2640 aactttatac atagaaaaaa ctgagtcgtt aggaacgtat attagtgcaa cacccagtac 2700 ctggctcaca gattcacgaa ttggagcaac cacctgatca gctaaataat caccaaacct 2760 gtctaaagca aatacgcaag ctatacggca aagtaagtca tcaaagtact ttttattcag 2820 ttgttcattt tctgcttcag attttccaac gacacgacca tatccgaaac cagcatatct 2880 aatgatttca cgcaatccca tacatgcgcc gtgtctaatt tcccaagaag ggtcaaacat 2940 atctataagc aataactcga caagagtttc gaaaggccat acggaatcac tagtcacagc 3000 taccgcagct gatggaacta taggagcctt atgctcaaca acgagacgat cagaccgact 3060 ttgagcagta aagttatagt ctgcaccagt ttttttatca gcagacgaag ttgagttttg 3120 ctgatgtact aaagttggag caacatcaa 3149 //