ID SPBC1815 standard; DNA; FUN; 4679 BP. XX AC AL050301; XX SV AL050301.1 XX DT 23-MAY-1999 (Rel. 59, Created) DT 23-MAY-1999 (Rel. 59, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c1815. XX KW cut2; eno1; enolase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-4679 RA Wood V., Rajandream M.A., Barrell B.G., Volckaert G.; RT ; RL Submitted (17-MAY-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Katholieke Universiteit Leuven, Laboratory of RL Gene Technology, Kardinaal Mercierlaan 92, B-3001 Leuven, Belgium. XX DR GOA; P21135; P21135. DR GOA; P40370; P40370. DR SWISS-PROT; P21135; CUT2_SCHPO. DR SWISS-PROT; P40370; ENO1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1815 is overlapped by cosmid c14C8, CC EMBL entry SPBC14C8, accession number AL022305 at the 3' end. XX FH Key Location/Qualifiers FH FT source 1..4679 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1815" FT /map="IIR" FT misc_feature complement(1..240) FT /note="1200 (-1) 1 240 c17G9 S. pombe unassigned" FT repeat_region 818..857 FT /label={taacc}8 FT /note="(taacc)8" FT CDS 2067..3386 FT /db_xref="GOA:P40370" FT /db_xref="SWISS-PROT:P40370" FT /label=eno1 FT /note="SPBC1815.01, len:439" FT /gene="eno1" FT /gene="SPBC1815.01" FT /product="enolase" FT /protein_id="CAB43486.1" FT /translation="MAIQKVFARQIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWE FT ALEMRDGDKTKWGGKGVLKAVGNVNNIIAPAVVKANLDVTDQKAADEFLLKLDGTENKS FT KLGANAILGVSMAICRAGAAQKKLPLWKYIAENFGTKGPYVLPVPSFNVLNGGSHAGGD FT LAFQEFMILPTGAPSFSEAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPDLQTPQ FT EALDLIVEAINKAGYEGKIKIGLDVASSEFYVDGKYDLDIKAAKPKPENKLTYQQLTDL FT YVELSKKYPIVSIEDPFDQDDWSAWTHMKAETDFQIVGDDLTVTNVKRLRTAIDKKCAN FT ALLLKVNQIGSVTESLNAVRMSYEAGWGVMVSHRSGETADTFISHLTVGIGAGQLKSGA FT PCRSERLAKYNELLRIEEELGSEGVYAGAHAGKYIKAAKF" FT CDS complement(4146..4661) FT /db_xref="GOA:P21135" FT /db_xref="SWISS-PROT:P21135" FT /label=cut2 FT /note="SPBC1815.02c, len:171" FT /gene="cut2" FT /gene="SPBC1815.02c" FT /product="cut2 protein." FT /protein_id="CAB43487.1" FT /translation="MPPPVHLDPIQSLGFDDVAIDCETLDPWPSMQNKATSVTIRNTPA FT SDFHVYKEFSDDDPIQFPLLSVDGDSPLTEKDTNLTTPATLKASDQQRKVLEKPSVSKQ FT SSSRTRLSTVYRTKLASGKSIPRPLSHKLTRPRVTASGNSRRRPLSRSIHSLSSSRIDF FT SSLDTGLL" FT misc_feature 4391..4679 FT /note="1445 (+1) 1 289 SPBC14C8 S. pombe chromosome 2" XX SQ Sequence 4679 BP; 1251 A; 1079 C; 1038 G; 1311 T; 0 other; gatggagaaa tggcaatgct gtaggagcca aaaggatcgt caacagagag gtgacatgct 60 actcggagga gaagagtgta gatttacagt aggagagaag cgggaggagg gagaatagaa 120 gaagaagaga ggagaattag taattggggg atttagtgtg tttatttcga ttgagatgaa 180 tgtttagtgg gatgaagaaa gtaatgaaag gatggatgaa aaaaaaagaa gcctgtgatc 240 aatagtattg tatttgatcg aaattcaaac aagatttcca aacattactc tacggtacat 300 tttgaaaaga gcaaagtatt tgtatgtcag gcaagggtca gagtcgagct agaaccagag 360 tcatagtaat aacgaaagtc atagcaatag ccaaaccaaa tccatagtca acgctactag 420 agccttcgcg aatatggacc aactcgtgac accgggatgc ttgcacatca ctacacttgt 480 gtttgctggt ccgcgttaac cttgtagtga atagcatgct gagtttcgct aacgcgctgc 540 tccgtgtcgc tgctgtgaat ctgatacagc gagcggttgg tgcagagcca ggaggattag 600 cgtccaaaag acgagacaag acgaggcgag ggacaagaaa tgagaacaaa gggaagagaa 660 gggacacaga ggaaggatcg accaagcaga tgtttgcaat tgagtagtag tgaattcaaa 720 aaaaaatttt tttaaagtat tttattctca atgtttaaat cccgtctgta atgtgattct 780 caagtagagt gagttgatgt gattctctga cccaccataa cctaacctaa cctaacctaa 840 cctaacctaa cctaacctga cctaacctga gcctgtatat cattctctat cccatcctgt 900 cctgtcttgg tcgtactgtc ctgcgctggc ctagtttgct ttgccctgcc ttgccttgcc 960 tcgttttgct cactccttcc cttcattgcg cactcttgcc tcccattgcc ctccactgcg 1020 attctcttgc aactgcctgc tgcaccgacc gccgcttcat tgcgatggtc tgctttgcgg 1080 caatttccat ccgtgcggta atgcccgttt cgttacgtta cctctggcgt gatcgttagg 1140 gtagtcaaac tgtgtcccgt atggcaccat gaagtttctc cggatggaac tatcctgatg 1200 tttatctgtc caaccaatcc atcctgcaac aagattttcc cctccaatca caacatttct 1260 ccggctttgc acttgctaaa ccttgatgac atcctaccac tgacatccgc gaatcaaggg 1320 acctctcgga acaactgatc tcatgaaccc gtccgctttt gcctttatct gcatcacccg 1380 aactgggcga ctgagggaag ggtggggcat caagtaacgc tcctttctcg gtggtttcct 1440 gcactgagca acgaacggta catgcagcga cgaggttgtt gtcgctgaca ttcgtcaaca 1500 aacatacaaa acggctcgct tcgtgtattg acgtccgcgt aatttcgctg cttctagctc 1560 ccgacctttt ccaaagatgg ccctcctcca gattactcac gttgtaagca acttttggga 1620 acactgttga ttacttggca aaaaaaaaca cttgccatat agttggttcc tcttttgacc 1680 acctacctaa cattaccttt ctctctctct ctctctacca acaaccacac cacaccacca 1740 caccactcca tacattacat taccttacca cactcacttc acttcatctc catctcttgc 1800 cccttctaag ctcgctggac tcgcacattg gctacggtgg ctcgcgtcgc tcgcttggtc 1860 gctccttcaa cgtaccctaa cgttccggtt aacgattctt aaagaagcga ccaatttgtt 1920 ccaccggctt ctgtgggctc aggggcatgt catggaagat tgagaggatc ggcgcttcct 1980 ccaggtatat atagagtgga agggccgtcc gttaggactt gtttcagtaa gaatcaatta 2040 gtattctaca gtaaacatcg ttaatcatgg ccattcagaa agtctttgct cgtcaaatct 2100 acgactctcg tggtaacccc actgtcgagg tcgacctcac caccgagacc ggtatccacc 2160 gtgctatcgt cccctctggt gcctccactg gtatatggga ggctcttgag atgcgtgatg 2220 gtgacaagac caagtggggt ggtaagggtg ttttgaaggc tgttggcaat gtcaacaaca 2280 tcattgcccc tgccgtcgtt aaggccaacc ttgacgttac cgaccaaaag gccgccgacg 2340 agttcttgct taagctcgac ggtactgaga acaagtccaa gttgggtgct aacgccatct 2400 tgggtgtctc catggctatc tgccgtgccg gtgctgccca aaagaagctc cctctctgga 2460 agtacattgc cgagaacttt ggcaccaagg gtccctatgt cctccccgtt ccctctttca 2520 acgtcttgaa cggtggttcc cacgctggtg gtgacttggc tttccaagaa ttcatgatcc 2580 tccccaccgg tgctccctct ttctccgagg ctatgcgttg gggtgctgag acctaccaca 2640 ctctcaagtc cattgccaag aagcgttatg gttcttctgc cggcaatgtc ggtgatgagg 2700 gtggtattgc tcccgatttg caaacccctc aagaggctct tgaccttatt gttgaggcca 2760 tcaacaaggc tggttacgag ggtaagatca agattggtct tgatgttgcc tcctccgagt 2820 tctacgttga cggcaagtac gaccttgaca tcaaggccgc caagcccaag cccgagaaca 2880 agcttaccta ccaacaactt accgaccttt acgtcgagct ctccaagaag taccccattg 2940 tctccattga ggaccccttc gaccaagatg actggtctgc ctggactcac atgaaggctg 3000 agaccgactt ccaaattgtc ggtgacgatt tgactgttac caacgtcaag cgtcttcgta 3060 ccgccatcga caagaagtgc gctaacgctc ttcttttgaa ggtcaaccaa atcggttccg 3120 tcaccgagtc tttgaacgcc gtccgcatgt cctacgaggc tggttggggt gttatggttt 3180 ctcaccgttc cggtgagacc gctgacacct tcatctccca cttgactgtt ggtattggtg 3240 ccggtcaact taagtctggt gctccttgcc gttccgagcg tttggccaag tacaacgagc 3300 ttctccgtat cgaggaggaa ctcggttccg agggtgttta cgctggtgcc catgctggca 3360 agtacatcaa ggctgctaag ttttaaactc tgagcataca ttaaatttta ctaatgttta 3420 caatacatgt ttatttttat agaatgcaat gaaattttta tcctttttgc gttttggatt 3480 aatttgccag gttgcgtgtt tatttattat tagtattaaa gtaaactgtt gttgtagtag 3540 aagtagctga tataattgtt ttgtgctaat ttgcatcctt ttttttttta gatgatcatt 3600 ttagttttcg tttatatagt gttttttttt caatcatctt aatttttttt ggaatgtgtg 3660 catctgctgt aaaacacaga aatttgtctt tgacctagta catattttta tgtgtagcca 3720 aaatttttgg atacctttgc tctttatcag tcactttact actactaaca ctggaaaatg 3780 ttctattcct cagcatctta cctactagta tttataatat tcatcaacgt agaatgaaaa 3840 taacaatatt attaacataa ttcatctaca ttacaatagt aaaatattga accagaaaag 3900 ccaaaaaaaa aaaagcatat agaaaaggaa atcatttgta cagaaaagtc atgaacgaaa 3960 aacatgtcat aaattaagga ccgtataggc tttatgcatt taagtaaaaa aaaaaaaaaa 4020 aagaacagca ttaaagtggt gaaacaaatt aaacaacaag ggaaatcaaa gccatcggaa 4080 gaatcatact tacaatcgta gaagcggaag ccagtacgca caaaacctga aaattcggaa 4140 tctttttata acaatcctgt atccaaagat gaaaaatcaa ttcgtgaaga tgataacgaa 4200 tgaatagaac gagataaagg gcgccgacgg ctattgccag atgcagttac tcggggtcgt 4260 gtgagtttat gagataaggg tcgagggatg gatttaccag atgccagctt ggtgcgataa 4320 acggtactga gtcgtgttct ggaagaggat tgtttgctga cagaaggctt ttcgagaacc 4380 tttctttgtt gatcagaagc tttcaaggta gcgggtgttg tcaggttagt atccttttct 4440 gttaacgggg agtcaccgtc tacgctgagc agaggaaatt gtataggatc atcatcactg 4500 aactccttgt aaacatggaa atctgatgca ggtgtattac gaatagttac tgaggtagcc 4560 ttattctgca tgctaggcca aggatcgagc gtttcacaat caattgcaac gtcgtcaaac 4620 cccagggatt gaatggggtc caaatgaaca ggaggaggca tatattcgat atcttcttc 4679 //