ID SPBC17F3 standard; DNA; FUN; 7300 BP. XX AC AL031263; XX SV AL031263.2 XX DT 12-AUG-1998 (Rel. 56, Created) DT 03-NOV-1999 (Rel. 61, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c17F3. XX KW GTPase-activator protein; Rho-like GTPases; KW serine/threonine protein kinase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-7300 RA Wood V., Rajandream M.A., Barrell B.G., Rieger M; RT ; RL Submitted (11-AUG-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Biotechnologische und molekularbiologische RL Forschung, Angelhofweg 39, D-69259 Wilhelmsfeld, Germany XX DR GOA; O75011; O75011. DR SPTREMBL; O74335; O74335. DR SWISS-PROT; O75011; NAK1_SCHPO. DR SWISS-PROT; Q09742; YB61_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 80% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c17F3 overlaps cosmid 21D10 at 3' end. XX FH Key Location/Qualifiers FH FT source 1..7300 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c17F3" FT /map="IIR" FT misc_feature complement(1..1016) FT /note="overlap with 1 1016 SPBC12C2 EM:Z54140 S. pombe FT chromosome 2" FT CDS 443..1399 FT /db_xref="SWISS-PROT:Q09742" FT /label=SPBC17F3.03c FT /note="SPBC17F3.03c, len:318" FT /product="hypothetical protein" FT /gene="SPBC12C2.01c" FT /gene="SPBC17F3.03c" FT /protein_id="CAA20325.1" FT /translation="MYNVTYRLLRLSLISSHSLRKSRSFDVKNFANIHFFIPLPNTPKK FT FYSVNAFSPLLKARRMTATIVSNHNVGCSCCYFRQYSTKQFRDLNTSEALSPCKAEPIP FT YKIMSSMSNFSDFKSRFSQYRESHLKLLNELQNVKDTNDLKDRIRLLHLNSSSKLNAFL FT HDLINAPNVSAEMKENCVRLIFFGRKYDPFLRSRMFTLTIRYFLLQKNRLRTSFYLLKH FT MELLALPASYELKTTLLEWFLRKRKFETAQKIFLSLCFSQHFPSEKLLMVLLLHGTETM FT QEYAMIQAINFVNTEQKLRFYDRLHSICSKLKFSSTG" FT CDS join(complement(3753..3832),complement(3215..3701), FT complement(1758..3149)) FT /db_xref="GOA:O75011" FT /db_xref="SWISS-PROT:O75011" FT /label=nak1 or SPBC17F3.02 FT /note="SPBC17F3.02, len:652" FT /gene="SPBC17F3.02" FT /gene="nak1" FT /product="n-terminal serine/threonine protein kinase." FT /protein_id="CAA20324.1" FT /translation="MENNTASSPYTKLELVGRGSYGAVYRGICNLTKETVAIKILNLDT FT DEDEVSDIQKEVAVLSELKQSDVENIIKYHGSYLVGTNLWIIMDYCHGGSVRTLMEAGP FT ISEPCISLILRETLQALKFIHHAGIIHRDIKAANILVSMSGNVKLCDFGVAAELNINRR FT KRITFIGTPYWMAPEVIRDGQEYNVMADIWSLGITAYEIATGSPPHAKEDPFRAVYLIA FT HTAPPRLNGNFSALLKEFIASCLQDVPQRRLDSSELLKSKFIKQYSRMSISELTNVVKR FT YDTWQAAGGIPQTLLLGEEADDGSDPDQETSDTAASDDGWEFGTIKQGQSNVSSITGTS FT TSTTTAATSSTTVTGTVIPKSSTVHEPPSSNDSHPLLQLFKDSKISDDDSPSNAEGAST FT EDSKGEVSYSQIELPSLDSSNLSSKKSTIQSKHTKQAEDYDLFVGRTRSNSKTSSDQSI FT KRPLPRVVQRQKTSLGKRGISMSPMKPGLRMPSSFDLQSRSISMGAFEQLSTPLEAPAH FT KHSAVLQPLEVNRSISIPPPKSISPSILHKPSLESASSTPKISSCSSTPKPFNSKLRAH FT LPPLSIGSPAVQPLANDNYDSLGVRGLNMELFNDYPGNMHNIKSVLSLEIDIVLGEMDA FT CLKSLECNLLNRKAYNE" FT misc_feature complement(2925..3149) FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 57.47" FT misc_feature complement(3150..3163) FT /note="ttaacaaagtttag, splice branch and acceptor" FT misc_feature complement(3209..3214) FT /note="gtatgt, splice donor sequence" FT misc_feature join(complement(3753..3805),complement(3224..3701)) FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 180.03" FT misc_feature complement(3702..3715) FT /note="ctaacattctaaag, splice branch and acceptor" FT misc_feature complement(3747..3752) FT /note="gtatga, splice donor sequence" FT CDS 6319..7300 FT /db_xref="SPTREMBL:O74335" FT /label=SPBC17F3.01c FT /note="SPBC17F3.01c, len:327, SIMILARITY:Saccharomyces FT cerevisiae, Q12128, yor3320w from chromosome xv., (409 aa), FT fasta scores: opt: 585, E():1.6e-30, (36.1% identity in 299 FT aa)" FT /partial FT /gene="SPBC17F3.01c" FT /product="RhoGAP GTPase activating protein" FT /protein_id="CAA20323.1" FT /translation="MTVVVGVLQIDDMGKKSSFASWWKQLRVKKSDDNKLFGLSLTRAL FT EVSKVAIFLTRRDGEKVFYGYIPAVVAKCGFFLKQNATQVKGIFRVNGSSKRIQILQKA FT FSTGPDYGRSFDWEGYTVHDAANVFRRFINLMPEHVIPLEYYERFREPMTIPNLTDNER FT VEMYRRRIDELPIPNRQLLLYLLDLLSVFAMNSSKNLMTADNLAAIFQPGILSHPDHEV FT YSKDYQISQTALLFLINHQGSFIKTIPLSSLPPLVAAPTSPNTSSTLITQSAAPTPIAS FT TSIRINSFNPRNGSIKRWRSFSRHSSATYSNSPSSNFSNMKSSEVD" FT misc_feature 6520..6981 FT /note="Match to PF00620 RhoGAP, RhoGAP domain Score 81.86" XX SQ Sequence 7300 BP; 2286 A; 1295 C; 1305 G; 2414 T; 0 other; aaattgcgta ccacaaaggt gtacttatat gtaataccca tgcttagcaa gttatgctca 60 acttccacat acaacgtatg caacgaacgg cagcctcatg ttatagtaaa ttagcaaatc 120 ctatataact aaacgaaagt gtatttgctt aacatatcgt ggagtttggg catatttctg 180 ctcacaagta aatctcatac aacatcgcat ttggttttaa tttgttttgg ttgttcgcaa 240 ggaagaagat tattctttaa aaaaaaggaa ataaatttat aaatataatt tgaaaaaaaa 300 tatatatata tatatttggg tttttacact cttttgacta cataactcga taggttttcg 360 gatttgttta atagacaaaa gaaggaagta cgtttaaggg tttatttcta tggaacatta 420 ttgatttttt ttttttcttg ctatgtacaa tgtgacatac aggttacttc gtctaagctt 480 gatttcttct cattctttaa gaaaatcacg gtccttcgat gtcaaaaact ttgcaaatat 540 tcattttttt attcccttac caaacacacc aaaaaagttt tattctgtga atgcattttc 600 ccctttacta aaagcacggc gcatgacagc tacgattgta agcaatcata atgttggttg 660 ttcatgttgc tactttcgac agtatagcac aaaacaattt cgtgatttaa ataccagtga 720 agctctttct ccatgtaaag ctgaaccgat accctataaa atcatgtctt ccatgagtaa 780 tttttcagat tttaagtctc gattttcaca atatcgagaa tcacatttaa agctactaaa 840 tgagcttcaa aatgttaaag acacaaatga tctaaaagac agaatacgct tacttcatct 900 caatagctct tctaaactta atgctttttt acacgatctt ataaacgcgc caaatgtgtc 960 agcagagatg aaggaaaatt gtgttcgtct gatttttttt ggaaggaagt atgatccttt 1020 tcttagatcg aggatgttca ctttaactat tcgttacttt ctacttcaaa aaaataggct 1080 taggactagt ttttatttat taaaacatat ggagttattg gctcttcctg cttcttatga 1140 attgaaaaca actcttttag agtggttctt acgaaaacga aaatttgaaa cagctcaaaa 1200 gattttccta tcgctctgct tttcccaaca ttttccctcc gaaaagttat tgatggtttt 1260 acttcttcat gggactgaaa ctatgcagga atatgcgatg atacaggcaa taaattttgt 1320 gaatacagaa caaaagttac ggttttacga tagactacac tcgatttgta gcaagttaaa 1380 gttttcctcg acgggttgat gctttggtaa taaaaatggt tcatttggat ggttatttaa 1440 atacaatatt aagatttcta ctgtttgggt atttagtaag gtaaattaaa tcattgtaat 1500 tttcgttcaa attgtaactc atataaatta aattatccct catcttgttc tttagttatt 1560 atcacgacaa taaatttacg actctattcc ttaaccgtgg gcactatttt aatttaaatg 1620 ttctgtattt ggcatcatta tctcaacaat ttagattttc ataaaagatg aacgaatata 1680 ttagcaaaaa aagagaagtt aaaacattaa ataaattaaa gattatatta tgaaaggact 1740 aaaagcatca taaacagtta ttcattatat gcttttctgt ttaaaagatt acattccaag 1800 ctctttagac aagcgtccat ttctcccaaa acgatgtcta tttctaagct caatacagac 1860 tttatattgt gcatattacc tggataatca ttaaataatt ccatatttag acctcgaacc 1920 cccagagaat cgtaattatc atttgctaat ggttgaacgg ccggtgagcc aatggataaa 1980 gggggtaaat gagctcttaa tttcgaatta aatggcttag gagtggacga acaggaggaa 2040 attttaggag tagaactagc ggattcaaga ctaggtttat gcaaaataga gggagaaatc 2100 gatttcggag gaggtatact aattgaccta ttaacctcta atggctgtag cactgccgaa 2160 tgtttatgcg ctggcgcttc taatggtgta gatagttgtt caaatgcgcc catcgatata 2220 cttctagatt ggaggtcgaa tgaggatggc atacgaagac ccggcttcat tggtgacatg 2280 gatattcctc gttttccaag agaagttttt tgacgttgca caactcgagg caggggtcgt 2340 ttgatacttt gatccgatga ggttttggaa tttgagcgtg tgcgacctac aaacaaatcg 2400 taatcttcag cttgcttagt gtgtttcgat tgaatggttg attttttgga ggatagattt 2460 gaggaatcta agctaggtaa ttcgatttga gaataagata cttcaccttt ggaatcctca 2520 gtagaagctc cttcagcgtt actgggagaa tcatcatccg aaatctttga atccttaaaa 2580 agttgtaata acggatggga atcattagat gaagggggtt catgcactgt ggaagatttt 2640 ggaattacag ttccagttac agtagtggag gaagtagcag cggtagtagt ggaggtggaa 2700 gtgccagtga tggatgaaac gttactttga ccttgtttaa tagtgccaaa ttcccaacca 2760 tcatcagaag cagcggtgtc ggaggtttcc tgatcagggt cgcttccatc atcggcttct 2820 tcacccaaga gtagcgtttg gggtatgccg ccagcagcct gccaagtgtc atagcgtttt 2880 acaacattgg taagttcaga aatagacatg cgagaatatt gtttgataaa ttttgatttc 2940 aaaagttcgg aagaatctaa tctcctctga gggacatcct gcaaacaaga ggcaatgaat 3000 tcttttaaaa gagcactaaa atttccattg agtctagggg gtgcggtgtg agcgattagg 3060 taaacggcac gaaaaggatc ctctttggca tgaggtggag atccagtagc aatttcatat 3120 gcagtgattc cgagcgacca aatgtcagcc taaactttgt taataatttt gtggaatagg 3180 taaagggcta taagtaatca gcaacgaaac ataccataac attatattcc tgaccatctc 3240 gaataacttc tggtgccatc caataaggcg ttccaataaa cgtgattcgc tttcggcgat 3300 tgatgtttag ttcggcagca acgccaaagt cacatagctt gacatttcca gacatggaaa 3360 ctaggatgtt ggcagctttt atatcacgat ggatgatacc tgcatgatga ataaatttta 3420 atgcctgtaa ggtctctcta aggatcaaag atatgcatgg ttcactaatg ggaccagcct 3480 ccatcaaagt acgcacgcta cctccatgac aataatccat tataatccat aaattagtac 3540 ctaccaaata agagccatga tactttataa tgttttcgac atcggactgt ttcaattcac 3600 taagaacagc gacttctttc tgaatatcag agacttcatc ctcatcggta tccaaattaa 3660 gaatcttgat ggcaacagtc tctttcgtta aattgcaaat tctttagaat gttagaaaat 3720 gtagaaaaga atcactcgaa taatattcat accctctata gactgcgccg tacgaaccgc 3780 gtcccaccaa ttctaatttt gtgtacggcg aagaagcagt gttattttcc atttctaata 3840 actttagctg cttagcagat acatgaagaa ttttaattga acaggttcaa ttcaaactcc 3900 ttcaattccc tttcactttt tctatactgc tggtggtagc gggtacgaag gaattgagta 3960 gatatatatt gaaaaactgt ttactataat actcaatttg tgcaatccct aaataacaaa 4020 caacttatta tactattttt tttcaatacc gaagctttgc gaggaaaaat acagcaaatt 4080 tagcaggaga aatgagaata tcttatcgag tttagcgtaa cgtaaaacta acgatatctt 4140 atctgtattt ttctactacg ctatgtcgtt aatcagtata taatctgtag ttatttatat 4200 ttccgttccg cagcggatat atatccgttc tttgagagta attatcttta agactaaaat 4260 cctttaaaat aaatcgaccg aatttttgtt cctcagttca aattgtaaac aatttataca 4320 gttatagtag tacccaatgt cttgtttata tccaaggata tttaaagccc atattcgtaa 4380 accaacttta cataatgcat acaatcatat aagatccttc tcacaatcta cattttacgg 4440 attgcaaaat ttccactatc taagcttaag agctgaaagc ttctcacctt acaaatatgc 4500 tgattttcgt agtaaataat aaaatgagat gacattactt atttactcgg tcgatttttg 4560 agaatttgcg taaaatacta atttgtatct taagataaag atactaagtt gattatgtaa 4620 atccgagtat gcatggttgt ctggtgtgtt acgtttagta tgctatccat tagcttgtac 4680 actgttctcc aaaccatttc atttttttta tgaaattacc aattggtttg taataagttg 4740 ctttttagtt aatttaacga gcattactgt tgctgctcat cttgcatggc tatcgtttcg 4800 aaccgatcat ataaaatatt cgacgaaaca atcgcataat aggagctagt caaagaaatt 4860 ttaaattttt gtacaatttc ttgagggcag cttagttttg tttgatggga tgacaaatcc 4920 cggtatttac agactttcaa ttcaatgaat tgatttgatg cgtccaatac ccacatcgct 4980 attttaaaag tgtagtaaat aaaaataatg taatgtatat tattaattta ttattacatt 5040 gccgagtaaa aagggagtaa aacgagaaat caattgaagg atttgtattt tctttattct 5100 ttcgtttact aatttttgta gcgatgacct attttaaagg ttagataaag taagtcaaca 5160 cgctgagctt aattgagatt atcggtgcga gcgatcgatt gtaacaatca atcgcaacaa 5220 tcaaatgata atgtattaag caaaaaaagg aaacttgaaa gaaatataga aatgagcgac 5280 ccttagctct tcgacctggg tgaacaagag tcgttgtaca taataaaaat ataacaaaaa 5340 agaaaacccc agacaaatca cagtttcaag catattatac aatcatagaa atttgcatta 5400 ctaaaagaat ttctcttaaa agattaatta tctaaacatt cctgtatatc gaaaatcgta 5460 gttgtatgta cagagagaca tggtactttt atttatcaat aaaaacagta taccaacaac 5520 caaggggagg gcattcttca ttgtcaaaac tcgtcagttt gacttgtaag aattcgcaat 5580 tcaaatattt cctaggaaca tgactttcaa ctcgtccttt aatgagccca ctttgcactg 5640 ctaatctggg atgggtggta tcagcacact tcctactgac actaacgcta atgtgccatg 5700 atcgtaaaat tgcaaaatct tcgctatcgc aagcagaatg tttgttttct ccgaagtgtt 5760 aaggaatgta tcgaatcgta tggtaaaatc attccatcat ccaaaggtca gtatacgata 5820 ttggcacgct aatgcgtaca ggacaatgct aagcctctct ctttacgagt tgctcgcttg 5880 aacgacttat aaattccttt aaattagtaa ccaagcgagt agtttggtat tttattagag 5940 gatttgcctt ccccgagcat cctcggaagt accggctgtg aaactgcgac acccaccttg 6000 caatagctaa ctagtactta ccaaccccgt agtgtgaacc tgctttgtac acaatacaac 6060 aaataattct aaaagcgcaa tttattaagt taagtttacc catctagtgg agaacagtgt 6120 tatttgagtt gaacagaatt gttattcaat tttaaagagt gtcaatttat cggttacttt 6180 ttttttacac atctacaatt ctttcattgt atttgggttt aaatcttttg tctgttatct 6240 cattttcctc attgaattct tttttttatt gttttcattt gttaagtttt tcaattttta 6300 ttacaatagc cattgttcat gactgttgtc gtaggagtcc tacagattga cgatatgggg 6360 aaaaagtcat cattcgctag ctggtggaag caattgcggg ttaaaaagtc agatgacaat 6420 aaattgtttg gtctgagcct tacaagggct ttggaagtat cgaaggtcgc tatctttctg 6480 acacgccgag acggtgagaa agtattttat ggctacattc ccgctgtcgt tgctaagtgc 6540 gggttttttc ttaaacaaaa tgctacccaa gtaaagggaa tttttcgcgt taatggttct 6600 tccaaacgaa tccaaattct ccaaaaagct ttcagtacag gcccggatta tggccgaagt 6660 tttgattggg aaggatacac agttcatgat gctgccaatg tttttcggag attcatcaat 6720 ctaatgccag agcatgtgat tcctttggag tattatgaaa ggtttcgtga acccatgaca 6780 attcccaatc ttacagacaa tgagcgagtg gaaatgtatc gtcgacgcat tgatgaatta 6840 ccgattccaa atcgccagct tttactttat ttactggact tactttccgt ttttgcgatg 6900 aactcgtcga agaacctgat gactgctgat aatttggctg ctattttcca acctggaatt 6960 ctatctcatc ctgatcacga ggtttactca aaagattatc aaatatcgca aacggctctt 7020 ttgttcctta tcaatcatca aggaagcttt atcaaaacca ttcctttgtc ttcactacct 7080 cctctggtag ctgcgcctac ttctccaaat acttcttcta ctttaattac gcaatctgct 7140 gcacctactc ctatagcctc gacatccatt aggattaatt cttttaatcc aagaaatgga 7200 tccattaagc gttggcgctc ttttagtcgt cattctagtg ccacttattc caatagtccg 7260 agttcaaatt tttcaaacat gaaaagctcc gaggtggatc 7300 //