ID SPBC1706 standard; DNA; FUN; 9000 BP. XX AC AL023533; XX SV AL023533.1 XX DT 11-MAY-1998 (Rel. 55, Created) DT 01-DEC-1999 (Rel. 61, Last updated, Version 4) XX DE S.pombe chromosome II cosmid c1706. XX KW SRC domain. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-9000 RA Lyne M., Rajandream M.A., Barrell B.G., Rieger M; RT ; RL Submitted (05-MAY-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Biotechnologische und molekularbiologische RL Forschung, Angelhofweg 39, D-69259 Wilhelmsfeld, Germany XX DR SPTREMBL; O60132; O60132. DR SPTREMBL; Q9USY7; Q9USY7. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 80% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1706 is overlapped at the 3' end by cosmid 1198 (contained CC in EMBL entry SP33010 accession number U33010). XX FH Key Location/Qualifiers FH FT source 1..9000 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1706" FT /map="IIL" FT misc_feature 1..624 FT /note="nominal overlap with cosmid SPBC354, EM:AL022071 FT S.pombe chromosome 2" FT CDS 948..3377 FT /db_xref="SPTREMBL:O60132" FT /label=SPBC1706.01 FT /note="SPBC1706.01, len:808, SIMILARITY:Saccharomyces FT cerevisiae, YAI4_YEAST, hypothetical 78.3 kd protein, (702 FT aa), fasta scores: opt: 288, E():2e-09, (22.4% identity in FT 539 aa)" FT /gene="SPBC1706.01" FT /product="SH3 Src homology domain protein" FT /protein_id="CAA19002.1" FT /translation="MEIMESHFDPTQQNDSTIIESRYSPEEYLEQSFEIQRIISGENSE FT PQTVASQEISDSQEEDTTLTSSQFEDCGTEYNEVVEDDEFRSEDEDDFMDEEEEYALYE FT AELSSSPSIHEEVIDCNFVHAIRGFEATVEGQVDATKGDMMILLDDSNSYWWLVKMCKN FT LAIGYLPAEYIETPSERLARLNKYKNSETSNSQQSVTLPPLDIVEKTLEAPSPNFRIKR FT VTFTCSSNSSDDEMDSENDYEAMVNRTVAENGLEIEFSDSSDSSLSAEYRSESEDHVTD FT SPAYVDLTELEGGFNQFNSTSFQSTSPLGLEIVETEINGSSTTADSKNSHSPYSKFSSA FT YPDAENSNISKINISIAGNKELYGNATQSDPSLYSTWIANKHKTASSATVDSPLRRSLS FT VDAMQSNASFSSYSSTSNTDKSLRPSSYSAVSESSNFTHDVSRDNKEISLNAPKSIIVS FT QSDSFDTSNVTQDAPNDVEKEPISGQMPNNLSVQSLKQLEVYPIRHSVSIEMPSEKLLS FT PRLYSSSTPSSPTKGFQKDDEEDSENRKQADKVELSPSSLLRQMSLPVDSSSQSDAQCT FT TSSVYITAERKAFSQSSIDLSTLSNHHVNNEINRRSFAGGFTSLADELSEMRELLHESP FT APLECNEEMVIPTPELDASSAIPSSSISHDEDLLPRKNTEESTSSSSFSSLITSPASLQ FT YDENPFKQSVVAELNNNSSSVPFVDSAHASDIHAYDNDHVSTKNKEFNRRLREFILDPD FT SLSGLYWSVKSAGVRASRRVSRNIEGESVSSDLDDIFANVLKGLSDEMASLLNTNR" FT misc_feature 1308..1478 FT /note="Pfam match to entry SH3 PF00018, Src homology domain FT 3" FT misc_feature complement(4298..4747) FT /note="possible RNA by identity to Japanese cDNA sequencing FT project clone" FT misc_feature complement(4833..6255) FT /note="similar at the DNA level to WTF hitchiker element FT region" FT CDS join(complement(5620..5758),complement(5494..5551), FT complement(5371..5444)) FT /label=SPBC1706.02c FT /note="SPBC1706.02c, len:70" FT /pseudo FT /gene="SPBC1706.02c" FT /product="wtf pseudogene" FT LTR complement(6007..6359) FT /note="TF1 type" FT CDS 6655..8931 FT /db_xref="SPTREMBL:Q9USY7" FT /label=SPBC1706.03 FT /note="SPBC1706.03, len:757, SIMILARITY:Saccharomyces FT cerevisiae, YB29_YEAST, hypothetical 97.8 kd protein, (855 FT aa), fasta scores: opt: 553, E():3.9e-27, (32.0% identity FT in 819 aa)" FT /gene="SPBC1706.03" FT /gene="SPBC839.01" FT /product="hypothetical protein" FT /protein_id="CAA19004.1" FT /translation="MEKSARQLSVAEQNGESGRLNNGYTSNNIQQYKDETNRHQFEYNQ FT NRNQLLRSIHIIQNLLNELDNYVDRSDCLFHSVWRTDKEKSKFSGNYYPFSPSKMNVIT FT IDLSLRSSSTADEKLISQLGEEAHESLLKVHIEKANKHLFSLFSRVEDTSSKILITGDL FT NAGKSTLCNALVHKDILPEDQQPCTEVFCEVHDAELNDGKDCVHAIPHGLTYSHTDSST FT YKVFPIEDLKRLVYETENWSMLIVYVNDGRPAHESLLHNGITDIALIDAPGLNTDSMKT FT TSVFACQEEIDVVVFVVNAENHFTLSATDFLRNASTEKSHIFIIVNKFDNIRDKERCKR FT LILEQIHTLSPGTFADAKDLVHFVSCRVARDPNNREDALYSSFFQMENSLRSFILENRS FT KSKLAPVRRYLSGLVGDILNICEYNIKLIDFDINHLQQRLTDLSPKFRKVKHEQQFTYQ FT KNESLVEATVQSISQHTHSELEDAIDSLGSFASVKYSGFFFAYQYAISVRDAMQQYLEE FT KLLESEDYARKRTEEAVLCIQKDVKDNFDSAVLPVFHANQMFIKKHRLQLQKHFRFELG FT LLDFIDLDLTERLGTWSASLSTILLVLGKTTPSFTTLGAFTGNLGYPIFKYFQNNSLQH FT LLVPVLGLASICVFGYVIYDIPRALPLKVAEKIKKSLRETDFCHNASIWIGTESRKVLN FT IPLNDLRRMFHQQWDKQRETISVAENDLRICQKARKFFGEIESRTREAKKKIMMVQLEG FT CDINY" FT misc_feature 6809..9000 FT /note="nominal overlap with cosmid SPBC839, EM:AL096796 S. FT pombe chromosome 2" FT misc_feature 7135..7158 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" XX SQ Sequence 9000 BP; 2970 A; 1549 C; 1532 G; 2949 T; 0 other; atctttctag gctaccttat ttttgtgttg aatactaatt gattcttctt cattcgaaat 60 attacgagca ttgaatgtat acacattaac aattaacatc aactttataa catccagacg 120 attatgtcgg tttactgttt tcgaataaaa acagggacat tttctatacc ctttacatcg 180 aactttatgt cctgaattaa tattacaaag catgctaagt aaaacctttt tttcaattat 240 tttagatatg catcatatag ctaactcgtt ggctttaaga gaactaaagc gtcacagctt 300 actgaggttg tttttgatac ttagatgcaa ttcaaacatt tataaataat taaatttgac 360 aatctaatta gaaataacat aaaaaaaaaa atcaaaatag aataaaatga tcaagaagct 420 ttttgtttaa atgcaaatac cgtactcgct tttattcgtt gtatcggtta aacgacgttt 480 tactgttacg ttatctgcca atctcggatt tacgtagcat caagtacgca tatcttaccg 540 caatatttag aggcgactta gtttgttaat atattatatt tttacactag ttattggaat 600 gttaacattt tgaagagcaa gatcgtttta ttttcaaaaa gattttgctg gattcgttgt 660 tacgattgac gttctaatca acccaacttg agtgtatgtt catcaggatc ggtatttccg 720 ttttcactag cgttttagcc agtatcctga atttttacca gttataaatt gtatgaggtt 780 gagagtttct tatccctttg caacgtgttt gtttttatca ttaggagtag cggtttttga 840 gacctgaaaa gtgtgtttgc tgtttttgca catttgttca agttcatgag agctaactac 900 attgtttcaa tatgttacac atgaatagtg ccagtagtgc tgacagcatg gaaattatgg 960 aaagtcattt tgacccgaca caacagaatg atagcactat aatagaaagt cgttactctc 1020 cggaagaata tctggagcag tcattcgaga tacaaaggat tatatcagga gaaaatagtg 1080 aaccccaaac tgtagcgtcg caagaaatta gcgattctca agaagaagac actacattaa 1140 catcgtctca atttgaggat tgtgggactg aatataatga agtagtggaa gatgatgagt 1200 ttcgctctga agatgaggat gattttatgg acgaagagga agaatacgct ttatatgagg 1260 cagaattgag ttcttcccca tctattcacg aagaggttat tgattgcaac tttgttcatg 1320 ccattcgagg cttcgaagct actgttgaag gacaagttga tgctactaaa ggagacatga 1380 tgattttatt agatgattct aatagttatt ggtggcttgt aaaaatgtgc aaaaatttgg 1440 ctataggcta tttacctgct gaatacattg aaactccttc agagcgtttg gctcgattaa 1500 ataaatataa aaattcggaa acgtccaatt ctcagcagtc tgtgactctc ccgcctttag 1560 atattgtgga aaaaaccttg gaggctccat ccccaaattt tcgtataaaa cgcgttacct 1620 ttacttgttc ttctaattct tccgatgatg aaatggattc tgaaaatgat tatgaggcaa 1680 tggtaaaccg aactgtcgca gagaatggtc ttgaaataga attttcggac agtagtgatt 1740 cttctctctc tgctgagtac cgttccgaat ccgaggatca tgtaactgat agtcctgctt 1800 acgtggatct tactgaactg gaaggtgggt ttaaccagtt taactctact tcttttcaaa 1860 gtacatcccc ccttggtctt gagattgttg aaacagaaat taatggctct tccactactg 1920 cagattcgaa aaattcacat tccccctact ctaaattctc atcagcttac ccagatgctg 1980 aaaattcgaa tattagtaaa ataaatatct caatagcagg aaacaaggaa ttatatggca 2040 atgccaccca gtctgatcct tcactttaca gtacttggat agcaaataag cataaaactg 2100 cttcatctgc aaccgttgat tctccattgc gaagatcact atcagttgac gcaatgcaat 2160 cgaatgcttc attttcttct tattcgtcta catctaatac tgataaatca ttgcggcctt 2220 cttcttattc cgccgtatcg gaatcctcta actttactca tgatgtatct cgtgataaca 2280 aggaaatctc attgaatgca ccaaaatcaa ttatagtttc tcagagtgac tcttttgaca 2340 catctaatgt tactcaggat gccccaaacg atgtagaaaa ggaaccaatt tctggtcaaa 2400 tgcccaataa tttatcagtt caatccttaa aacaattaga agtgtaccct attcgacatt 2460 cggtatcgat tgaaatgcca tctgaaaagc ttctttcccc tcgattatac tcttcatcta 2520 ctccatcctc tccaacaaaa ggattccaaa aggatgacga agaagacagc gaaaaccgaa 2580 agcaagcaga taaggttgaa ttatcaccaa gttcgcttct tcgtcagatg tctttacctg 2640 ttgattcttc ctcgcaaagt gatgcacagt gcacaacgtc ttctgtatac ataacagcgg 2700 agcgtaaagc attttctcaa tcttcaatag atttatcaac attgagtaac catcatgtaa 2760 ataacgaaat aaacagaaga agttttgcgg gtggatttac aagtttagca gatgaattaa 2820 gcgaaatgcg tgagctacta cacgaatccc cggctccatt ggagtgtaat gaggaaatgg 2880 ttatacctac acctgagtta gatgcttcaa gtgcaatccc gagctcttcg atttcacacg 2940 atgaggattt gctaccaaga aagaacacag aggaatctac ctcttctagt tccttttcct 3000 ctcttatcac ttctcctgct tctttgcaat atgatgaaaa tccattcaag caatctgtgg 3060 ttgcggaatt aaataataac tcctcttcgg tgccattcgt tgattcggct catgcttctg 3120 atatccatgc gtacgataac gaccacgtta gtactaaaaa caaagaattt aatcgtcggt 3180 tgagagagtt tatattggat ccagactctc ttagcgggct atattggagt gtaaaatcag 3240 cgggagtaag ggcatcccgc cgagtttctc gtaatatcga gggtgaatcg gtgtcctcgg 3300 acttggatga tatttttgct aacgtattga agggtttatc agatgagatg gcctccctct 3360 taaatacgaa tcgctgaaat ctttgattaa tttaaaagtt ataccatgtt tttccccttt 3420 atcaaactat ttacactcgt tataaatcag ttttctgttt gattaaactg tttcaaaaat 3480 ttgtttgtta atgtctttat gtttgacttt tttttcttgt taataatatt ttttaataac 3540 taacagcata ccatagttgt ataaaggtct aaaattcaat gtgcgggtat gattcggatt 3600 attgagcgct ataaaataaa tgtgtttaag taaaatctac cgaagtatta aatgagctag 3660 taattatctt atatgccatt tcaaattata gtatcgctgc atcatatttt ttgtacagta 3720 atgcgagcag tgaaaatgta aactactcaa gcagatagta gtaggctttg agggtatgat 3780 agattttatg catccgtaaa aataagccag tgtactttac ttatgtggca tgatgaatca 3840 aattcaatag ggtattaaaa aacaaaatta gcacgattta tagcgattct tattaaaaaa 3900 tatttctctt ctcttttaaa cgaaaaaaat gagatattaa caaacgatga acatgttcat 3960 taatcattaa tatgagaata tcatctgctt ctacttttaa ggtatcggat tcatcatcaa 4020 accaacggtg tatttaaatg tatattttgt aagcgatagg catattgatt gtctcataat 4080 aaataattta tgttttttca ctggtaattt tcacgctaaa ttagatgata attagcatct 4140 tgactaccaa tttctcacca tcatcataat tgaagggcga aattttcaaa tttgactttt 4200 aatattcttt tagtaattgc acgaagatat gtaaaggaac atggaaaagt aagaatgaac 4260 aataaaattt ttattccttt acaaatttac gacttgttat taattggaaa gacaaagcaa 4320 tgttatttct gtaacgaatt gagtttgtag gaagaaacaa agttcctaaa aagtatgcaa 4380 atatcttcaa tatgcgctaa ataaagaaaa cagttcgtac agtaaacaaa gtcaataatt 4440 cgaagcgact aaataacgaa aaatttagaa tgagacggat atgaaatgca ttttgaattg 4500 acccgttttc atttgtacaa ccaccataca tgattattct caacattgtt ttttatgaat 4560 gcgaaataag aagtattagt ttaaaacatc aagtaatttt ttaacacgcc accacgagtg 4620 gacaatgtga agtttacaac ttgtgctaat ttttttgact actcaaaaaa ttaaatatgc 4680 agcacaaaac gagaactgaa ttatatgcaa acccttatac actgagcctt aaaattcatg 4740 attttttact tgcaacagaa agtaataagt agcttaaaaa attagaggaa atctgttaat 4800 cgttacagtg gattctcaca ttagacaata actacattat tacgataaca aatcatcgtt 4860 ctaaatcaca atattttatt atgctgttca ttattttgta aacattaatt ttgtttgtat 4920 taaagtaaac cactaaacca aaggaaaaaa atgataatta agtttgattc attaagaatc 4980 aagatgaaga agaaatgaat gcaataaacg acaatgaaat tgtgaattta acaaggcgaa 5040 acaacgagtt tttcgatttc attagttgat gataacagtc aattagtgaa ctgctatttt 5100 aaaacgaata aataaaacca ttattataat attcaatgcc cagtgtatgt tattcacgga 5160 acctttcaag caatgcactt gctcatccaa ttcgtcagga aatgaaatat gcaataaaaa 5220 tacatgtgac atactttaaa cagatttatg atttgtttga gtaaatcaca atcataaagg 5280 cgcttcttta ttgcacattc ttatgaagaa aagaagcctc gtgtaaaaag cgactttatc 5340 atacaattag taaagttaac aatataatta ggcttcactt tcggggttca tctcacgcaa 5400 tggaacagct gtgtattcat tcacttgctg tttggcctca taacctaatg ataattagca 5460 tacacaatta aaaagatatc atattaaatt tacccatctt tctgatcaag tgttggatat 5520 gccaactgta aacaatgatc acatccaagt tattttacag cactggcaag tttcaacttt 5580 caattgagta attcctttct tattatttac aattcttacc tgaatatgga ggcaatgtac 5640 tttcctcctc tgaatcatat tctgggaggg gtcctttttc caaatcgatt tcatgatctg 5700 tcttcaattc atcttcttca tctagaggac ttttcaaaga agtgtaatta ttcttcataa 5760 taataaaatc attctttatt taataaaata catgcgcgaa ttaattgcta tgattgcgac 5820 aaaccaaaca gcgaaatcaa gaatattcac tttgtagtcc aattccaatc gctttaaaga 5880 ctttttaaca aaataataaa atcgttcaaa caaatttcca actaattcct tcaaacaatg 5940 gcataaaaaa agaagaacga aaaaactgaa cgaggcagtg aattacttct gacacattta 6000 ttttgtcact gcccttttta ttcaccccaa cttagttttc cttatacagc atactgttaa 6060 aattggttcg ctacgttgcg tgtcactaat ttcatatttg taaaatcatg ttttttaata 6120 tagacatcag ttaagaacaa agacttagaa gattataaat ggaattagtc gctgcatctc 6180 attcctcagt tcaattatta gctaaatcgg tgactgatac tgttaggcgt cttataacaa 6240 taaatataat attgtcagtt aacataacgc acaacgctat atgatttatg tatcgctaaa 6300 acagatatca aacgtcgtcg atgtcaacag tatacggtat acttgaaatt tcgttcctac 6360 gtcttattta tgatgataca aatgtaaatt tttgaattgt tgtgctcaag gatataatag 6420 aaatttcttt tttaaagctt tatatgtaga aagatgatgt ctttattttc tcatattcat 6480 ctactaatat gttgctccca ttttttttca ttattcgtac taaggtgtta tgcaaacagt 6540 atatggttgc aacgcatatg tattaatttt taaggaagtt gctctttgca tgaaatttcc 6600 aatctttagg gatcaagttc aattatataa acgcaaaatt caactttaaa tgatatggag 6660 aagagtgcaa ggcaattatc agtagccgaa cagaatggtg aatcgggaag attaaataat 6720 ggatatactt caaacaatat tcagcagtat aaagatgaaa ctaatcgtca tcagttcgag 6780 tataaccaaa ataggaacca attgttaaga tccatccata ttattcaaaa tctgttaaat 6840 gaactggata attatgtaga tcgatctgac tgtttgttcc acagtgtttg gagaaccgat 6900 aaagaaaaat ctaaattttc aggaaattac tatccattta gtccgtccaa aatgaatgtg 6960 atcaccattg atctatcact gcggtcgtcg tcgactgctg atgaaaaatt aatcagtcaa 7020 ttgggcgagg aagctcatga atctctacta aaagtacaca tagagaaagc aaataagcat 7080 ctcttttcac ttttttctag agtagaagat acctcttcga aaatccttat caccggggat 7140 ttaaacgcag gtaaatcgac cctttgcaat gctttggtac acaaagacat tctaccagaa 7200 gatcaacaac cttgtactga ggtattttgt gaagtgcacg atgctgaact caatgatgga 7260 aaagattgtg ttcacgctat tcctcatgga ctcacgtata gtcatactga ctcttcaaca 7320 tataaagttt ttccaatcga ggatttgaaa cgccttgttt atgagacgga aaattggagc 7380 atgctaattg tatatgtcaa tgatggaaga ccggcacatg agagtttgct tcacaatgga 7440 attacagata ttgcacttat tgatgctcct ggcttgaata ctgattccat gaagacaact 7500 tcagtatttg cttgccaaga ggaaattgac gttgtcgttt tcgttgtaaa cgctgaaaat 7560 cattttaccc tttctgctac agatttctta agaaacgcct ccactgagaa atcccacatt 7620 ttcattattg tgaacaaatt tgataacatc agagataaag agagatgtaa acgattgatc 7680 cttgaacaaa ttcataccct ttctcctggt acttttgctg acgctaaaga cttggtacat 7740 tttgtgagct gtcgtgtcgc tcgtgatcca aataatcgag aggatgctct atattcttct 7800 ttttttcaga tggaaaactc tctccgtagc ttcattctcg aaaatcgctc aaaatctaaa 7860 cttgccccgg tacgtagata cttgtcaggg ttagtcggtg atatacttaa tatctgcgaa 7920 tataacataa aacttatcga ttttgatatt aatcacttgc aacaacgttt gacggatctt 7980 tctccaaaat ttcgtaaggt aaaacatgag cagcaattta cctatcagaa gaatgaatct 8040 ttagttgaag cgactgtgca atctatttct cagcatacac attccgaatt agaagatgct 8100 attgactcat taggttcttt tgcttctgtc aagtattctg gttttttctt tgcctaccag 8160 tatgcgatat ccgtaagaga cgccatgcaa caatatttag aagaaaaatt gttggagtca 8220 gaagattatg ctcgaaagag gaccgaagaa gctgtcttat gtattcaaaa agatgtaaaa 8280 gataattttg actccgccgt ccttccagtc tttcatgcca accaaatgtt tattaagaaa 8340 catagattgc aactgcagaa gcattttcga tttgaacttg gcttgcttga ttttatagat 8400 ctagacctta ctgagagatt aggaacctgg agcgcttcat tgagtactat tctcttggtc 8460 cttggaaaaa caaccccttc atttacaacg cttggtgcat ttactggaaa tcttggttat 8520 ccaatattta aatattttca aaataactcg cttcaacact tattggtccc ggtacttggt 8580 ttagcaagta tttgcgtttt tgggtatgtt atttacgata tacctagagc acttcctctt 8640 aaagttgcgg aaaagataaa aaaatcttta agggagacag atttttgtca caatgctagt 8700 atttggatag gtacagagag tcgtaaagtt ctcaatattc cgttgaatga tttacgaaga 8760 atgtttcacc aacaatggga taagcagcgc gagactatat ctgtcgctga gaatgactta 8820 cgaatttgtc aaaaagcaag aaagtttttt ggtgaaatag aaagccgaac tcgtgaagct 8880 aagaagaaga taatgatggt tcaattggag ggatgtgata taaattatta gcagtacggg 8940 ctgaggctaa gttttaaaaa aaaagaaaag aaaattggaa gtggccttcc ttactgaaaa 9000 //