ID SPBC1215 standard; DNA; FUN; 6490 BP. XX AC AL096846; XX SV AL096846.1 XX DT 16-JUL-1999 (Rel. 60, Created) DT 16-JUL-1999 (Rel. 60, Last updated, Version 1) XX DE S.pombe chromosome II cosmid c1215. XX KW actin cytoskeleton organisation; COX complex biogenesis; DEC1 homologue; KW mitochondrial inheritance; SURF-family protein. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6490 RA Lyne M.H., Rajandream M.A., Barrell B.G., Seeger K., Quail M., Harris D.; RT ; RL Submitted (16-JUL-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and XX DR GOA; Q9Y810; Q9Y810. DR SPTREMBL; Q9Y809; Q9Y809. DR SWISS-PROT; Q9Y810; SUR1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1215 is overlapped at the 5' end by cosmid c1750, CC EMBL entry SPAB4534, accession number AB004534, and at the 3' CC end by cosmid c83, EMBL entry SPBC83, accession number AL035536. XX FH Key Location/Qualifiers FH FT source 1..6490 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1215" FT /map="IIL" FT misc_feature complement(1..82) FT /note="nominal overlap with cosmid SPAB4534 (c1750) S. FT pombe chromosome 2" FT tRNA 36..107 FT /note="tRNA Thr anticodon TGT, Cove score 78.51" FT rRNA 415..581 FT /note="SPRG5SD K00771 Yeast (s.pombe) 5s rrna gene and FT flanks" FT CDS join(1501..1760,1844..2456) FT /db_xref="GOA:Q9Y810" FT /db_xref="SWISS-PROT:Q9Y810" FT /label=SPBC1215.01 FT /note="SPBC1215.01, len:290, SIMILARITY:Saccharomyces FT cerevisiae, YG2X_YEAST, hypothetical 45.1 kd protein in FT clb6-spt6 intergenic region, (389 aa), fasta scores: opt: FT 385, E():2.8e-19, (29.0% identity in 314 aa)" FT /gene="SPBC1215.01" FT /product="putative SURF-family protein" FT /protein_id="CAB50922.1" FT /translation="MFWWKSATKFTFSKRGPCVFRYLSTLEGTTVRPKKNKFLVGLLSA FT VPIVTFALGTWQVKRREWKMGIINTLTERLQQPAILLPKTVTEQDTKKLEWTRVLLRGV FT FCHDQEMLVGPRTKEGQPGYHVVTPFILDDGRRILVNRGWIARSFAEQSSRDPSSLPKG FT PVVIEGLLRQHTDKPRFMMKNEPEKNSFYFLNVREFAQLKGTLPILITELQPSLTPLQE FT ADHVKRGLPLGHPLKVEIFNSHTEYIITWYSLSVVSAIMLYVYFKRGSGTSSLNSAYER FT SKILNNKRL" FT misc_feature 1761..1766 FT /note="gtacgt, splice donor sequence" FT misc_feature 1827..1843 FT /note="ctaacataatcacttag, splice branch and acceptor" FT CDS join(complement(5434..5466),complement(5251..5389), FT complement(4889..5007),complement(4413..4849), FT complement(4111..4372),complement(2953..4071), FT complement(2663..2881),complement(2495..2602)) FT /db_xref="SPTREMBL:Q9Y809" FT /label=SPBC1215.02c FT /note="SPBC1215.02c, len:811, SIMILARITY:Saccharomyces FT cerevisiae, DEC1_YEAST, dec1 protein, (796 aa), fasta FT scores: opt: 184, E():0.00014, (23.4% identity in 577 aa)" FT /gene="SPBC1215.02c" FT /product="similar to yeast DEC1 mitochondrial inheritance FT and actin cytoskeleton organisation protein" FT /protein_id="CAB50923.1" FT /translation="MRRSGSKESTIVYSALSLAQAGRGPEALALLEPLKSTPINSLELL FT DIIQAVYDDQKKGEESFVFWEKFLQTYGKQEKNLLAYFKASIRIKSLSHQRKAAVELQK FT NFPSRKHTLWVISSLYLLSKKSENEVEQRLLKALAEKTAKLIFEKPTGYIDSCEEFHLY FT LDVLLLVGDKDRALDALIHQDADRFVDADADLLLRKLELLASCARWDSLFTFSLSLFQT FT GNTDWKVCKALLDSASNDDSKLVPLKDCILKALSTSSTKRNLHLLWIEASARFFPEEHE FT SALLGYIKKLYMKPIVFEDLRPYLLKLNVDAQHRLLDAFKLADLGESNESQKVDKLYAE FT VLLLKIHFLLFESFTAESVVDYVRRCFVAFEKGLSLSKGLLPTDFTHGYEALLLAVHSL FT IYMWEGNKDLKPAEKQALIFDAICLLEKGITYSQHNFHLKLPLIRLYLLLDGGFPAAAK FT VYDTMSIKQIQNDTLDHYLLTRATTYYPSSVTSHYINSSLKIYGSNEFETPEMISMAYE FT DGAYSQIEDMRNFRSRLDHSTWKSISLVERARIHYLTAFKPPKQYLPKCSSPKDNRDLK FT VFADYGSDKLPTVEESLRNSPKPDTLWIHLTVIGHSLVQDSIVNGDFEKAVLSAKEMEV FT LCENNDLSKQLTSEEIVHMKLLIQLGLLSVKVKNGDYENSSFETIENLIESFDYENSTP FT LSQLTKYTEIINDLITCLNSFLYHVSATKKKEFTRQYQLLKNISSNKLGSISGITKHKK FT KAARKYVSELLSNSWLSNLSETQVPYDPKFAKQVGEGMIDSYIQTTDAVSKLPKFVKF" FT misc_feature complement(2603..2612) FT /note="ctaataatag, splice branch and acceptor" FT misc_feature complement(2657..2662) FT /note="gtacgt, splice donor sequence" FT misc_feature complement(2882..2896) FT /note="ctaaccgaattatag, splice branch and acceptor" FT misc_feature complement(2947..2952) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(4072..4085) FT /note="ttaactcgtcgtag, splice branch and acceptor" FT misc_feature complement(4105..4110) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(4373..4387) FT /note="ctaacttctccatag, splice branch and acceptor" FT misc_feature complement(4407..4412) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(4850..4861) FT /note="ctaactcctcag, splice branch and acceptor" FT misc_feature complement(4883..4888) FT /note="gtaaga, splice donor sequence" FT misc_feature complement(5008..5021) FT /note="ttaactatttgaag, splice branch and acceptor" FT misc_feature complement(5245..5250) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(5390..5405) FT /note="ttaacatcctttttag, splice branch and acceptor" FT misc_feature complement(5428..5433) FT /note="gtaagt, splice donor sequence" FT misc_feature 6387..6490 FT /note="nominal overlap with cosmid SPBC83 S. pombe FT chromosome 2" XX SQ Sequence 6490 BP; 2097 A; 1138 C; 1138 G; 2117 T; 0 other; tatatataat ttaataaata cattccgacg atactgcctc tatggcttag tggtacagca 60 tcgcacttgt aatgcgaaga tccttggttc gattccgagt ggaggcatat acattatatt 120 atattctttt tcatgcggaa aaaagatttc aaatttttgg gtatgatatt aatatgactg 180 taacgttaat agcaaagtga gtgttaataa tgataaaata gcagcaaaat ctcttttccg 240 agtaagacgt tttccagtct aaatttggag tctgcagttg tttcgcaatt cttaatgtat 300 ggttatacta aatacaaact ttaaagctct gatttatgtt tgcaataaac taaaataaaa 360 gcacaaaaac ctttacccat taatttcaaa caacttataa actaccggta aacttttttt 420 ctaaccttta taatttataa actagaatgt ttaatgtcta cggccatacc taggcgaaaa 480 caccagttcc cgtccgatca ctgcagttaa gcgtctgagg gcctcgttag tactatggtt 540 ggagacaaca tgggaatccg gggtgctgta ggctattttt ttatatccgt ctttcttact 600 acttgcctaa caagtcatga tgtactctca aaatatgttt gcatgccttg taatattggt 660 tatggatagc tccttctgga cttgatcttt tgtagccaag aacaatgggt atagactctg 720 accttgtgat gttgtagcca cagattataa taggtatttt caagtacagt aacaaaaatc 780 ttctagtttt tttttagaaa ggatacacca agtataagca aattcaggaa ttgttgatta 840 aactgtcaac ttcggtaaaa ctttgggcat aagtagtgtg ggagcaagtt taactaaaat 900 tctattcaga tgtcgaatcc aaaccgctaa ttttgctcaa ctagcttttc ataaaaacca 960 attcatagtt tcatactaat aaagacgatt gtttacttta aaacatacgt cgtaagaaca 1020 tatattgctt tatcgaaaga taacaaatgt taagctatta tattatttaa ctatagcgca 1080 gatttcgctt cctttactta aaaaagacat gtgacttgta gaagcttgga gtgaatacgc 1140 aaaggtacct acttagacat tcgcgtctct cttagctgtc aacatcaaca aactggcccc 1200 gtattgaaca gtatcttact tgtcgaagga tttgactaag aaaattttat ttctttatag 1260 caatattccg ttttcgctta gaagattcta gtcaattgcc ctattctact tacgctttac 1320 agtagtatca gaagacctga gtgggatttt gctgctagta gaggccattc aagttaactc 1380 cgttttgcaa cattttaaaa gtttttgaat tgaatataaa tatcaattgt ttgattcctt 1440 ttaggattta atcttttctt tttatttttg tttcgattga atcttggatt cctgtctatc 1500 atgttttggt ggaaaagtgc tactaaattc acattctcaa agcgtggacc gtgtgtcttt 1560 cgctatttga gtactcttga aggaacaact gtgaggccta aaaaaaataa atttttagtt 1620 ggattgcttt ctgccgttcc aattgtcacg tttgctttag gaacttggca ggtaaagcga 1680 cgagaatgga aaatgggtat catcaataca ctcacggaaa ggcttcaaca gcccgcaatt 1740 ttattaccga aaactgttac gtacgttaag ttaacatata cacaaattgc acgttttgca 1800 attgaactgt cgttttttac attaagctaa cataatcact tagagagcaa gatacaaaaa 1860 aacttgagtg gactagggtt ttgcttcgtg gtgtgttttg tcacgaccaa gaaatgttgg 1920 tcggtccaag aacgaaggaa ggccaacctg gctatcacgt agtaacccca tttattttag 1980 acgatgggcg tcgaatttta gtcaacagag gatggattgc tcgatcattt gctgaacagt 2040 cttctcgaga tcctagttct ttacctaaag gtccagtggt cattgaaggt cttttgagac 2100 aacatactga taagccaaga tttatgatga agaatgagcc tgaaaaaaat tctttttact 2160 tcttaaatgt tcgtgagttt gcacaattga aaggaactct ccccattttg ataacagaac 2220 tacaaccatc gcttacaccg ttgcaagaag ccgatcatgt taagagaggc ttgcctcttg 2280 gtcatcctct aaaagttgaa attttcaaca gtcatacaga atatattatc acttggtatt 2340 ctctaagtgt ggtatcagct ataatgcttt acgtctattt taagagaggt tcaggcacat 2400 cttctctgaa ttctgcatac gaaagaagca agattctaaa caacaaacga ttataaaaaa 2460 ttttcatatt tataagtttc taaatattat ctacctaaaa ttttacaaat tttggaagct 2520 tgcttactgc gtccgtcgtt tgaatgtatg aatcgatcat tccttcacca acttgttttg 2580 caaacttcgg atcgtaagga acctattatt aggaagttag ttctcatgcc tataatttta 2640 atgctctata atcatcacgt acctgagttt cagataaatt gctcagccaa gagtttgaca 2700 acaattctga tacatatttt ctggcggcct tctttttatg cttcgtaatt ccagagatac 2760 ttcctaattt gttactgctg atgtttttaa gaagttggta ttgtctggta aattcttttt 2820 tcttggtagc tgacacgtga tataggaagc tgtttagaca agtgataagg tcatttatga 2880 tctataattc ggttagttta gaaattttta tatcattttt tttaaaaaaa aaaaccagtt 2940 tggtaaactt acttcagtgt actttgtcaa ttgacttaaa ggcgtggagt tctcataatc 3000 gaatgactca attaaatttt caatcgtttc gaacgaggaa ttttcgtagt ctccgttctt 3060 gacctttacc gaaagtaatc ccagttggat caataatttc atatgaacaa tttcttccga 3120 agttaattgc ttcgataaat cattgttttc gcacaaaact tccatttcct tagcacttaa 3180 aacggccttt tcaaaatcgc cgtttactat gctgtcttga acgagagaat gaccaatgac 3240 agtcaaatgg atccaaagcg tgtccggttt aggcgaattt cttaaacttt cttcaactgt 3300 tggcaattta tcagatccat aatctgcaaa gacctttaaa tctcgattat ctttcggaga 3360 tgaacatttg ggaagatact gtttgggtgg tttaaaagcg gtaagataat gtatcctagc 3420 ccgctcgacc aaagaaatgc ttttccaggt actgtggtct aaacgggatc ggaaattacg 3480 catatcttcg atttgagagt aagcaccatc ttcataagcc atagaaatca tctctggtgt 3540 ttcaaactca ttcgaaccat atattttgag acttgagtta atatagtgag aagtaacaga 3600 agatggataa taggtagtag cacgagtcaa gagataatga tcaagggtat cgttttgaat 3660 ttgcttaatg gacatagtgt cgtaaacttt agcagcagca gggaagcctc catcaagaag 3720 aagatataaa cgaataaggg gtagctttaa atgaaagtta tgttgactat aagtaatgcc 3780 tttttctaaa agacagattg cgtcaaaaat taaagcttgt ttttcggcag gttttaaatc 3840 cttattcccc tcccacatat atatcaatga atgaactgct aataacaaag cttcataacc 3900 gtgcgtaaag tcagtgggta ataagccttt acttaaagac aaaccctttt caaatgcgac 3960 gaagcatcta cgcacgtaat cgacaaccga ttctgcagta aacgactcaa agagcaaaaa 4020 atggatcttc agaagcaaaa cctcagcata taatttatcg actttttgag actacgacga 4080 gttaatattg aaagatgaat aaatacttac ttcattggat tcacctagat cagccaactt 4140 aaatgcatct aataaccggt gttgtgcatc aacattcagc ttcaatagat aaggtctcaa 4200 atcttcaaaa acaattggtt tcatatacag ctttttgata tagccaagta atgctgattc 4260 gtgttcttca ggaaaaaacc gtgcgctggc ttcaatccaa agtaaatgaa gatttctttt 4320 tgtgctgctg gttgacaacg ctttcaatat acaatccttt aatgggacta atctatggag 4380 aagttagtaa taatgcaaaa aaaaaaactt actttgaatc atcattggaa gcactatcca 4440 gcaaagcttt gcaaaccttc caatcagtat tcccagtttg gaaaagagaa agcgaaaacg 4500 tgaataaaga atcccaacgc gcacaggaag caagcaattc aagcttcctc aaaagaaggt 4560 cggcgtcagc atcaacaaag cgatcagcat cttgatgtat taaagcatct aacgctctgt 4620 ccttgtctcc cactaaaagc aacacgtcta aataaagatg aaactcctca caagaatcga 4680 tataacctgt tggtttttcg aaaatgagtt tagcagtttt ttcagctagt gctttcagca 4740 aacgttgttc aacctcattt tccgactttt tggacaaaag atacaaagat gagataaccc 4800 ataaggtatg tttcctagaa ggaaagttct tttgcaattc tacagcagcc tgaggagtta 4860 gtaaaaaaaa aacaaattaa gatcttacct ttctttggtg agacaatgat ttgatgcgaa 4920 tagatgcttt gaagtaagct aataagtttt tttcctgctt tccataagtt tgcaagaact 4980 tttcccaaaa tacaaacgat tcttctcctt caaatagtta ataacttaaa agtaagaggg 5040 taaaaacaag tccattgaga aataaaacgt tgcacttgaa tcatcaagct acgaaaagaa 5100 caaagatcgt ttttcttaag caaagtttaa ccgatcaaat aactaaagcc tttatctcaa 5160 ggaaatagta gaatattcaa aataaaaaat acgaccaaag tgctatcaac atttatgttc 5220 atctgttcaa tagaacctaa ttatacttac ccttcttttg atcatcataa acagcttgta 5280 taatgtctaa aagttccaag ctgttgatgg gagtcgattt taacggttct aaaagtgcta 5340 gagcctcggg accacgtcct gcctgagcca aggataacgc tgaataaacc taaaaaggat 5400 gttaacagct aaaaacataa ctcgattact tacaattgtt gattctttac tcccagaacg 5460 acgcattgta gtaggcaatg aaattaaagc ccaaacagta gtcagaagtt ggtaggtgtt 5520 gcagtcaaat tattattcta cagaggagaa tattatagcc agcgtggtag aatctggata 5580 tatatctact gcaaaagtgt aattgcattg gtttaaaggg tatactatgg ttaagtaata 5640 tattcacagc tgtacaattt acagtcataa ctaaaacttc cttaagccgt aaagaaatac 5700 ctggtgttgt aaaatttgtt gtatatccac ggcatggtca tataatgtga ttttgtgctc 5760 aaataaatat aaaatatgca taatttttgt acatttaatt tgagaaaccc atcttttgtt 5820 gagaggctgt caatgaatag cagtttcatt gaaaagcagc gggatgacca gaaaagtatt 5880 ttacaatggc aagggagtag aaagctagcg taatattcag aaagctaggt aattgagcaa 5940 tcctttaatt cattgctaag catgctaggt aaacgcagta aacctttcag ttttcattta 6000 ggtataaggc tgtttaatga gtatctccac taaatttaaa gatcaaaact cagtatcaat 6060 tcttaaaagt tttattttat ttaataatca tatacttctc ataatctttc aattttttcc 6120 ccattttgat gatattttta ttaatcctac agtaagctct atgatatcgt tattcttcaa 6180 ataggctggt cagcacgtgg acggtgttac ttatcgttaa ataaatcgta ctaaggaggt 6240 gcgatgtaaa tgatatgctt gtcaagtatt aactgctctc caccaaccgc cggtttaact 6300 gattattgtt gaaaagcgca gacgaagttt agagaattac tagcgtattt taaatttaat 6360 caacggacta ttttttattc ctttgagatc cgactttatc gctttgcttc taattttcca 6420 aaattcagtc tatctacgcg atccagccct gtttgcgtaa atttcatatt atttttcttt 6480 aaacgtttgg 6490 //