ID SPBC115 standard; DNA; FUN; 7000 BP. XX AC AL022105; XX SV AL022105.2 XX DT 11-MAR-1998 (Rel. 54, Created) DT 08-NOV-1999 (Rel. 61, Last updated, Version 2) XX DE S.pombe chromosome II cosmid c115. XX KW ATP/GTP-binding site motif A (P-loop). XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-7000 RA Wood V., Rajandream M.A., Barrell B.G., Devlin K., Churcher C.M.; RT ; RL Submitted (04-MAR-1998) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O42894; O42894. DR GOA; O42895; O42895. DR GOA; O42896; O42896. DR SPTREMBL; O42894; O42894. DR SPTREMBL; O42895; O42895. DR SPTREMBL; O42896; O42896. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 80% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c115 is overlapped at the 5' end by cosmid c947. XX FH Key Location/Qualifiers FH FT source 1..7000 FT /chromosome="II" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c115" FT /map="IIL" FT misc_feature 1..1427 FT /note="Overlap with SPBC839 EM:AL096796 S.pombe chromosome FT II cosmid c839." FT CDS complement(789..1895) FT /db_xref="GOA:O42896" FT /db_xref="SPTREMBL:O42896" FT /label=SPBC115.03 FT /note="SPBC115.03, len:368, SIMILARITY:Schizosaccharomyces FT pombe, O13991, hypothetical 41.4 kd protein , (369 aa), FT fasta scores: opt: 1685, E():0, (67.4% identity in 368 aa)" FT /gene="SPBC115.03" FT /gene="SPBC839.18c" FT /product="Oxidoreductase" FT /protein_id="CAA17915.1" FT /translation="MPPIKTAVLGTGVSAFVFHFPFLEALPSKFEIYACLERRATVTQS FT KARSAYPNILVYTNLDELLADVNIELVVVSLPPNVHSEIVKKALNAGKHVVCEKPFTPT FT YEEAKELYELAESKSLLLAIYQNRRWDGDFLTAKKIIESGRLGQVVEFESHFDRYRLGR FT KSGSWKDEPRPGNGMVYGIGSHLIDQAVSLFGTPYSVTAKLEAQRQIPPLEVEDYFRII FT LHYPAKGNKLPINVILSSTNVSCGCEMRFCIKGTRGSFMKFGFDPQESQLHSGMKPNDH FT GFGTDRFELYGNLWTVPLDADVKALPEPTKITVPTVQGNYRDFYDAVFEEILKKANEFP FT IKSDQVLAVEKIMEAAYKSSESSSSIQL" FT misc_feature complement(1137..1886) FT /note="Match to PF01408 GFO_IDH_MocA, Oxidoreductase family FT Score 235.12" FT CDS 4099..5463 FT /db_xref="GOA:O42895" FT /db_xref="SPTREMBL:O42895" FT /label=SPBC115.02c FT /note="SPBC115.02c, len:454, SIMILARITY:Haematobia FT irritans, N2B_HAEIR, putative atpase n2b, (464 aa), fasta FT scores: opt: 678, E():0, (33.1% identity in 450 aa)" FT /gene="SPBC115.02c" FT /product="putative atpase" FT /protein_id="CAA17914.1" FT /translation="MLQVLRVSAPCFAFVDTIGSIGVCRVVRFTTFHNTPIEVYNKKVN FT DGVWKRDPYQETAVKAINRLYTELESYTQPPITQDSMPAEKGSILSWISPLKKMFSRKK FT SPTLTSSLPVPGMPKGIYLYGDVGCGKTALMDLFYHNLPPNVTRSQRIHFHAFMMQVHR FT TSHDLQDRYGFEIDFIDHIASGIAKETTVLCFDELQVTDVADALLLRRLFEALMKYGVV FT IFITSNRAPSDLYKNGIQRESFIPCIKLLEHRLQVICLDSPNDYRRLKSKTEDTYLYPA FT NSPEVKKALENWFLCYADEKDPAHQDEVEVFGRKIIVPKASGNVAWFTFEQLCGEPKSA FT ADYLSLASRYHVFIVSDIPKLSIESKDLIHRFITFIDALYDTHGKLILSSEVPVQEIYP FT TAPSEVLSSTADPAAKGKIESHYHGAFGGIEEVFTFTRCLSRLSEMKKQSWIHSP" FT misc_feature 4471..4494 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS join(5819..5873,5957..6582) FT /db_xref="GOA:O42894" FT /db_xref="SPTREMBL:O42894" FT /label=SPBC115.01c FT /note="SPBC115.01c, len:226, SIMILARITY:Saccharomyces FT cerevisiae, YG2N_YEAST, hypothetical 28.3 kd protein, (256 FT aa), fasta scores: opt: 300, E():6e-13, (32.5% identity in FT 212 aa)" FT /gene="SPBC115.01c" FT /product="hypothetical protein" FT /protein_id="CAA17913.1" FT /translation="MNRIGILSRSDGSSEWKQGSARVICGVNGPIDVKIRDERLNKATV FT EVLVQPVSGVAETLEKMISSRIVGILEDAIFLNTYPRTLIQVSIQIIEEDGTDTLAAVI FT NGAVLALLDAGISLKYIPCAINCHWKNKITQDEPDVDGTINKLESIITICYSISSEPAK FT LIFLETAGPIPEEDFFRVLETAPLHAEEVSKKMKELLFETYNESDGHENEKNPKEDVEM FT DVVA" FT misc_feature 5874..5879 FT /note="gtaagt, splice donor sequence" FT misc_feature 5942..5956 FT /note="ctaacatcattgtag, splice branch and acceptor" FT misc_feature 6899..7000 FT /note="nominal overlap with c947 EM:AL021837" XX SQ Sequence 7000 BP; 2317 A; 1216 C; 1141 G; 2326 T; 0 other; gttaaactta ttgtcagaac gaaaaataaa ttgttcatcg taagagagtt ataagataga 60 tttactttta tttccgaaaa cacttagtac atgctgtgaa attagcatgg aggatgaaca 120 gaatggcaat aaccgagtac cataaatcat acttctcgta aaagtatggt ttgtttgatt 180 caatctttat tatacagtat cctaatcaaa aagctctcaa atatagtttg ggtgtctgta 240 agaaaagcat atttcacttc atattgtttt ggtataccta tatactaaat agaaaatcaa 300 tgcatttacc aattttttta gctcgacaag aagaatcaat agtcagaata gagtgttgaa 360 attgtgaaaa cttcgttaat acgcaaacaa tatcatatga aacgagcatt aaaattttaa 420 tgctgcttaa acagataatt acagaatagc aacagtgaat cttttcacta gtatgacttt 480 attgtctttt gtacgttcag tcccagtggt cgtccactta ttggaaagga gctttaaata 540 tgaaaacaat attcccttca tttgaacatt atatgatttt ggtaatccca tttcaaattc 600 tttaaacggt ttctaaaaca tgcaattcat taccattgat tatggctatg agatgttata 660 acgcaattgt taaaaccaaa tattcactat tttaattcta agatcacttc tcatttagtt 720 taaagaaaaa caagcaagga ttttcacact gaagggttac ttcatttaat aaaattgtaa 780 atattctttt acaactggat cgagctagaa gattcagaac ttttatatgc agcctccatt 840 attttttcta ctgccaaaac ttgatcagat ttgataggaa actcgttagc tttttttaag 900 atttcctcaa aaaccgcatc gtagaagtcc cggtaatttc cctgaacagt aggaaccgta 960 attttagttg gttcaggcaa agctttcacg tcagcatcca aaggaacagt ccataagttt 1020 ccatagagct caaatctatc agttccaaat ccatgatcgt taggcttcat tcctgaatgt 1080 aattgtgact cttgtgggtc aaacccaaat ttcataaaag aaccacgagt gcccttaata 1140 cagaatcgca tttcacagcc gcatgacaca ttagtagaag acagaattac gttgattgga 1200 agtttgtttc ctttagcagg gtaatgaaga ataatacgaa aataatcttc cacctccaaa 1260 ggaggaattt gacgttgagc ttccagcttg gcagtaacag agtaaggcgt gccaaacaag 1320 gagactgctt gatcaattaa atgtgagcct atcccgtaca ccattccgtt cccgggtcta 1380 ggttcatctt tccaacttcc agattttcga cccagacggt atcgatcaaa gtgagattcg 1440 aattcaacaa cttgaccaag tcttccgctt tctattattt ttttagcagt cagaaaatca 1500 ccatcccacc gacgattttg ataaattgcc agtagtaggc tttttgattc ggcaagttca 1560 tagagctctt tggcttcctc ataggtagga gtaaacggct tttcacaaac cacatgctta 1620 ccagcgttca aagctttttt tacaatttca gaatgaacat tgggaggtag ggaaactacg 1680 accaactcaa tattgacgtc ggctaataat tcatccaagt ttgtataaac caaaatattg 1740 ggatatgcag agcgtgcttt ggattgtgta actgttgccc tacgttctaa acatgcataa 1800 atttcaaatt ttgaaggtaa agcttcaagg aatgggaaat gaaaaacaaa agctgacaca 1860 ccagttccaa gcactgcagt cttaatcgga ggcatagttt aacttaatca aagtaatgat 1920 ttgtaagctg tagaatacag aaattttttt aatacgagaa aatatcgaac cgctaaagga 1980 agaattttac taacgacagt gtaagcatat gatcagatat taataatcgg taattataaa 2040 attttaaccg aatgaaggga tccatttatc aaacataatc atcatttagc taaacatatg 2100 ctaaaatgca ttcatgtgaa tacttgaaag aaaatgtttg aaatcagtct attttacgaa 2160 ctttgatttt agacatacca gttacgcttt tttattataa caagaaagca gtaagatttc 2220 aattaaaaat tcaagtaata aaaaactgat ttttgctaat caatctggaa aaggttacag 2280 agtcaaagtg gatgaaataa actaaattta aaagtagttt gttttcgaca gctcgattac 2340 ctaacaaaga tcgcttatta gcatgtcatt aaaatattaa acactataac gatcattagg 2400 ttgagcttgt taaccatagt tatcatagct tttgctttgt aattaatatc atgccaatat 2460 ttcctcttat aaaaacggga attttcgaac aaataatgta catactatgg gataaacagt 2520 catctactca aagatatttt tataacattg agctttagcg tagttgatgc ctaagatcaa 2580 acaaagtaat gacttatggg aattttgagg aagtaaacat ttgaagtatc tgtttaagtg 2640 cttcttctaa aattctttat tgcctgcctt ctttcagaat acaaagtatc gccttaacaa 2700 ttcacttcgg ttttactatt tgaatggtgt tcgcttagat gagcttaaaa attttataga 2760 gtttattaat ttaaggcctt tactgcgtat gaagatagtt tttctttttc tttttttttt 2820 ttaagctaat ggcatcagtt tatatttctg agccaactta ctaccctttt ttttaaagta 2880 attagtgaaa atttaaaata tcattcatga attcacatgc taaaatagcg aatacatatg 2940 ccttatttta tctactaaga agctttcaaa gttcttaact caaacatttc agtattcctt 3000 attatcacgt tttaagaatt tccctccttt tttatatatc atttaacaac atttgttaca 3060 catacaagat cataacttca tgcagtagct gtgtgtaaac actagtatat taatatataa 3120 ttcatttatt tgtttgtggt ctactaaaga taaaataaaa taagaataaa cataagaaat 3180 attaataagc caaaccaaat aatcacaaaa aatacgtata taggcgtttt ataaatatct 3240 gcgttttgct ggaataatag ttatgaaact aatgaacgga attcaactaa acatactcta 3300 cagcaccaat taaattcact atcgatacga acttctttct tcgggatttc cttaaaatca 3360 cttaacatca attgtcaaac ttattaacaa actgaagcaa tacttgatat tgaccaaatt 3420 aatattattc tcgtactttt taattgagta caaaaaataa agttgccatt gtttgttgaa 3480 tatacaaaaa atggaaaaga aataaataat actttaataa catgagttcc aatgtattat 3540 tgaaattcca tgcgattaac ttttttacta atatacagaa taactccacc tatctgggat 3600 cgtatggaag tatagttgct tatacttagt tccattaaat ttaaaatttt ttaaacaaga 3660 aattttttta ttaagaacag gtttctattt accaacgaaa taggcttgac ttactagttt 3720 cagattaaca tttgctcatg attaagacaa tacgcaaacg catataccga aatcatcgca 3780 attcactata ataaaagcaa actgcactgc cgaacgctat tcagaggcaa gaataatcat 3840 gcatctgtcc aacgatttat atttattcac ttaaaaacat atgtctttaa tcactaagta 3900 cgtcttgtag ttgacatatt ttctttctaa tcttttgctt ttcatctaca atcgttctac 3960 agctttatat ccactctcac tttatcattc ctgaatgaat tcactaattg atcaataacc 4020 actttttttc tttactttag ttagcttacg aaacattatt tttgttcaaa agtttgtatt 4080 taatttggtc atatcatcat gctgcaagtg cttcgtgttt cagctccttg ttttgctttt 4140 gttgatacta taggaagcat tggagtttgc agggtagttc gctttaccac atttcataat 4200 acacctattg aagtatacaa caagaaagtt aatgacggcg tatggaaacg tgacccgtat 4260 caagaaacag cggttaaagc aatcaaccgt ttgtataccg aattggaatc gtatacacaa 4320 cctccaataa cgcaagatag tatgccagct gaaaagggat caatcctatc ttggatatcc 4380 cccttaaaga aaatgttttc taggaagaag tctcccactc taacttcttc tttacctgta 4440 ccgggtatgc ctaaaggtat atatctatat ggtgatgtgg gctgtggtaa aaccgctttg 4500 atggatctat tttatcacaa cttaccaccc aatgttactc gttctcagcg cattcatttc 4560 catgcattta tgatgcaagt tcatcgcact tcacatgatt tgcaagatcg ttatgggttt 4620 gagattgatt ttatagatca tattgcatcc ggaatagcaa aagaaacaac agtgctatgt 4680 tttgacgaat tacaggtaac cgatgtcgcg gacgctcttt tactacgcag actctttgag 4740 gcccttatga agtatggtgt tgttatcttt attacttcta atagggctcc ttctgatttg 4800 tataaaaatg gaatacaaag agagagcttc attccttgca ttaaactttt ggaacatcgt 4860 cttcaagtaa tttgtttgga cagtccaaac gattaccgac gcctaaaatc taaaacagaa 4920 gatacatatc tttatcctgc taatagccca gaagttaaga aagccttgga aaattggttt 4980 ctttgctacg ctgatgagaa agatcctgct caccaagacg aagttgaagt atttgggaga 5040 aagataatcg ttccaaaagc aagtggcaac gttgcatggt tcacctttga acaattatgt 5100 ggagaaccga aaagcgctgc cgactatctt tcattggcaa gccgttacca tgttttcatc 5160 gtttcagaca ttcccaaatt gagtatcgag tcgaaagacc ttatccatag gttcattaca 5220 tttattgatg ccctttatga cacccatgga aaactcatct tatcatctga agttccagtt 5280 caagaaatat atccgacagc accttctgaa gtactttcct ctacggctga tcctgctgcc 5340 aaggggaaaa ttgaatctca ctatcatgga gcatttggtg ggattgaaga ggtatttacc 5400 ttcacgcgtt gcttgagtag actttcagaa atgaaaaaac aatcctggat tcattctcct 5460 taatacaaag gtgattcctt tataatgaga ttatatatca tgatgtatta tttttaaaaa 5520 aatattttga aagatggcta tgagtaattt ctaatttttt agtggttggg ctgttacaga 5580 tcattccagt ttctcctatc attcagcttt agtaaagtaa aaattggaag aatttaatta 5640 caaaccctta ttaatacaac tcccattgtc accaaactat tgatacattt ttttctgctc 5700 tcattctgtt ttcgaataac gaggtgtaag ctagcttatt tcctacttgt gcaaaggaag 5760 tagaaacgtt tcttctgtct ctgcaagcaa gcaactttgt tgcattatag aagctgaaat 5820 gaaccgtata ggtatcctct caaggtctga tggctcgagc gaatggaaac aaggtaatgc 5880 tattatccac ggtatattag ccaagcaaat gccaattaaa aaaaaatgaa ttttctttat 5940 gctaacatca ttgtaggttc tgctagggtt atttgtggtg tcaatggtcc tattgatgta 6000 aaaatcaggg acgaaaggct taacaaggcc accgttgaag ttttggttca acctgtgagt 6060 ggtgtcgctg agactttgga aaagatgatt tctagcagaa ttgttggtat cctagaagat 6120 gcaatatttc taaacacata tccaagaaca cttatacaag tgtcaattca gataatagag 6180 gaagatggca cagatacatt ggctgccgta atcaatggtg cggtacttgc tttattggac 6240 gcaggtatct ctttaaaata tattccctgt gcaataaatt gtcactggaa gaacaaaata 6300 actcaagatg aacctgatgt cgatgggaca attaacaaat tggagtctat tattactatt 6360 tgttattcaa tttcctcaga accagccaaa ttaatttttc tcgaaacagc tggccccatt 6420 ccagaagaag attttttccg tgttctggag acagcaccat tgcatgccga ggaagtttct 6480 aagaaaatga aagaattgct ttttgaaacg tacaacgaga gcgatggtca tgaaaatgaa 6540 aagaacccca aagaggatgt tgagatggat gtggttgcat aagaaaatca ataaagtgca 6600 tgtaaaaagt ccatctcaag gaccttcgtt tcgtatttac cattgtacct gttaaacgac 6660 tatacctttg aacaatatct ttggctgttc atttttatac ttagatttta tattacttgg 6720 gcgatataca ttgggttatt tttaatttat tttagtttat tactaattga gaaaccagaa 6780 cctaaacaat cctactacat tagaaggttt actgtcattc tttcaattca ctagcaatta 6840 atgtgttctt tcaatacttc aatgtatgct gatacatttg ggttaaatca taaaagaaga 6900 tctgtcataa gacaatcgtt cagtacaagg ccttgggtat atacactcat gacttttttt 6960 tttaattttg ccactcactc acttactcta tacatattga 7000 //