ID SPAPJ736 standard; DNA; FUN; 2734 BP. XX AC AL157872; XX SV AL157872.1 XX DT 24-FEB-2000 (Rel. 62, Created) DT 24-FEB-2000 (Rel. 62, Last updated, Version 1) XX DE S.pombe chromosome I PCR product pJ736. XX KW serine-rich protein; serine/threonine protein kinase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-2734 RA Saunders D., Harris D., Lyne M., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (23-FEB-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk. XX DR GOA; Q96UP3; Q96UP3. DR GOA; Q9P7P4; Q9P7P4. DR SPTREMBL; Q9P7P4; Q9P7P4. DR SWISS-PROT; Q96UP3; RIF1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC PCR product pJ736 is overlapped at the 5' end by cosmid c6F6, CC EMBL entry SPAC6F6, accession number Z98981 and at the 3' end by cosmid CC c1805, EMBL entry SPAC1805, accession number AL117390. XX FH Key Location/Qualifiers FH FT source 1..2734 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /map="IL" FT misc_feature 1..128 FT /note="nominal overlap with cosmid SPAC6F6 Z98981 S.pombe FT chromosome I" FT CDS 1..2317 FT /codon_start=2 FT /db_xref="GOA:Q96UP3" FT /db_xref="SWISS-PROT:Q96UP3" FT /label=SPAPJ736.01 FT /note="SPAPJ736.01, len:>771" FT /partial FT /gene="SPAPJ736.01" FT /gene="SPAC6F6.17" FT /product="hypothetical serine-rich protein" FT /protein_id="CAB75989.1" FT /translation="FALMVKSAIEAFGINTFVEASYLVSDNQLVLIDQTKAIDSYDHVP FT ISPLIYLLHSLALLTNGTLFSTVHAAYSSILSSIEEYHLRFGLKLMLLWDCVSPLSDDG FT TLVLARVLVSHEVSRLTSEALLSELKSRNGNNSVEEGFSEEERSILLKLLSWNVKFCSI FT SDAGSVNNLLQQYFTAIYKFEGCGSVFPFVVDPFTTILDDVLSFEANKVYSFAISLMKV FT STFESCTKELPPVTLPENIRQHLDSYNHMVELYNTLLQRLSSSDQVDLQCRYLHELSEF FT IKKIPKEFIFHTICKLSKGLIPCFLMNAFPQLEKSTTLQKSCTNFCILILQLLLNSTAT FT ASNILESLSPLLTSGLKSISKEVVLAAIKFWNQVFGKFESEEYPIELQKTISYLSKTYI FT ILLPFQSLCPGGKQANHQSSEKMSDILKGVDELRSVSKNGPYASSQDKGEKTTEFSGPG FT KPNNDNYIQIASVQELDDSSKGKAGKMPASKKNKRQKGDVKKIDETKNEATDMEESLTT FT PSGKVNKEVIVDDTSLRDEAIVPDKAIDVADNSNALLKENISSQSNRKADNNGTPSVNN FT SFTTANNDECSKENSQIEPEGQTASREGVLSTPRSTRKKRKLGRKSQSSNVNKEVAISE FT VSATLENVEVIERHGISEQGQNLDESACVLTNESSLSQTEIPEEKTENETTAVNGFENS FT KKRQFSSLLSGSIDTNNESNKVSSVEFDKSGPQDIIQSMTEATFEIEKNIQDLKSEEVQ FT KLSDLLMRLQRAILSRIA" FT CDS join(complement(2681..2734),complement(2449..2640)) FT /codon_start=1 FT /db_xref="GOA:Q9P7P4" FT /db_xref="SPTREMBL:Q9P7P4" FT /label=SPAPJ736.02c FT /note="SPAPJ736.02c, len:81, SIMILARITY:Saccharomyces FT cerevisiae, KOE5_YEAST, probable serine/threonine-protein FT kinase yol045w, (1101 aa), fasta scores: opt: 209, FT E():5.9e-08, (50.0% identity in 66 aa)" FT /partial FT /gene="SPAPJ736.02c" FT /gene="SPAC1805.01c" FT /product="probable serine/threonine-protein kinase" FT /protein_id="CAB75990.1" FT /translation="ALGILLYTIVYRENPYYNIEEILDAKLRIPFELSKDNVDLICRML FT DRNVHDRITIEETLQHHWFDDIRYLDTSHIRIPLSS" FT misc_feature complement(2543..2734) FT /note="Pfam match to entry PF00069 pkinase, Eukaryotic FT protein kinase domain, score 41.00, E-value 2.8e-10" FT misc_feature 2638..2734 FT /note="nominal overlap with cosmid SPAC1805 AL117390 FT S.pombe chromosome I" FT misc_feature complement(2641..2651) FT /note="ctaattattag, splice branch and acceptor" FT misc_feature complement(2675..2680) FT /note="gtaaga, splice donor sequence" XX SQ Sequence 2734 BP; 924 A; 470 C; 502 G; 838 T; 0 other; gtttgcatta atggttaagt ctgccattga agcttttggt attaatacat tcgtcgaagc 60 atcatatctg gtttccgata atcagcttgt tttaatagat cagacaaaag caatcgatag 120 ttatgatcat gtcccaatta gccccttaat ttacttgctc cactctctcg ctcttttaac 180 aaacggcaca ttattttcga ctgtacatgc cgcttacagc agtattctgt catccattga 240 agaatatcat ttaagatttg gtttaaaact tatgttactt tgggattgtg tatctccttt 300 gagtgacgat ggaactttag tgcttgcgag agttctcgtt tcccatgaag tatctaggtt 360 gacctcggag gctctgcttt cagaacttaa aagtcggaat ggaaataatt cagtagaaga 420 aggattttca gaagaggagc gatccatatt acttaaacta ttaagttgga atgtgaagtt 480 ttgcagtatt agtgatgctg gtagtgtaaa caatcttctt caacaatact ttaccgctat 540 ttataaattt gaaggatgtg gtagcgtttt tccttttgtc gttgatcctt ttactacaat 600 cctcgatgat gtattaagtt ttgaagcaaa taaagtttat tcatttgcca tatctttgat 660 gaaagtttct acttttgaaa gttgcacaaa ggagctacca cctgtaacat tacctgaaaa 720 tatcagacaa cacctggata gttacaacca catggtcgaa ttgtataata cactgttaca 780 aaggttatca agttccgatc aagtagatct acagtgtcgc tatcttcatg agctttctga 840 atttataaag aaaataccca aagagttcat ttttcatact atttgtaagt tgtctaaagg 900 tttgatacct tgcttcctta tgaacgcttt tccacaatta gagaaatcta ccacattaca 960 aaaatcatgt accaactttt gtatattaat tttgcagctg ctactaaatt ccactgctac 1020 agcttctaat attttagaat ctctttctcc gctgttgaca tcaggtctta aaagtatttc 1080 aaaagaggtg gtccttgctg ctatcaagtt ttggaatcaa gtttttggta aatttgaatc 1140 ggaagaatac cctattgaac tacaaaaaac aatatcgtat ctgagcaaaa cttatattat 1200 tcttttaccg tttcagtcac tatgtcctgg tggtaagcaa gccaaccatc aatcgagcga 1260 aaagatgtcg gatatattaa aaggagttga tgagttgcga tctgtctcta agaatggtcc 1320 ttatgcttct tcacaagata aaggagaaaa gactaccgaa ttctctggtc ctggtaaacc 1380 taataacgac aattacatac agattgcttc tgtgcaagaa ttagacgaca gctcaaaagg 1440 gaaagcagga aaaatgccag cgtctaagaa aaataaaaga caaaaaggtg atgttaaaaa 1500 aattgatgaa acaaaaaatg aagctactga tatggaagag tctttgacaa caccgtctgg 1560 caaggtcaat aaggaagtaa ttgtagacga tacttcttta agagatgaag caattgtacc 1620 tgataaagct atagatgtgg cagacaactc taacgctctt ttaaaggaaa atatttcaag 1680 ccaaagcaat aggaaagctg ataataatgg aacgccgagt gtcaataact ccttcactac 1740 agcaaataat gatgaatgtt caaaagaaaa ttctcagata gaaccagaag ggcaaactgc 1800 ctctcgtgaa ggtgttctat ctactcctcg cagtactaga aagaaacgaa aacttggacg 1860 taagagccaa tcttccaacg tgaataagga agttgcaatt agcgaagtgt ccgcaacctt 1920 ggaaaatgtg gaagtcatag aacgacatgg gatttcagaa cagggacaga atctggacga 1980 atctgcttgt gtacttacta atgaaagtag tttgagccaa actgaaatac cagaggaaaa 2040 aaccgagaat gaaacaactg cagttaacgg atttgaaaat tccaagaaac gacaattttc 2100 gagcttactt tcagggtcca ttgatacaaa caatgaatcc aacaaagttt catctgtaga 2160 gtttgataaa tctggtcctc aagacattat acaatcaatg acggaagcca cttttgaaat 2220 cgaaaagaat atacaagatt taaaatcgga ggaagttcag aagcttagtg atttgttgat 2280 gagattacag agagctattt tatctagaat tgcttgatta ttcaactcat gggtacagga 2340 attacggacc ggcattgggg tcaatttagt cataaatcaa tcttattaat ggggttcata 2400 ctggcattta atatataaca tatgtatata gataagaact ttctagtttc aagaggatag 2460 aggtatgcgt atatgtgatg tgtccaaata tcttatatca tcaaaccaat gatgttgtaa 2520 cgtctcttca atagtaatgc gatcatgcac atttctatca agcattcgac aaattaaatc 2580 gacattatct ttcgatagtt caaaaggaat tctaagtttg gcgtccaaaa tctcttcgat 2640 ctaataatta gttagcatac cattataata attctcttac gttataataa gggttttccc 2700 gataaactat cgtgtataat aaaataccca aagc 2734 //