ID SPAPJ735 standard; DNA; FUN; 1862 BP. XX AC AL163031; XX SV AL163031.1 XX DT 04-APR-2000 (Rel. 63, Created) DT 04-APR-2000 (Rel. 63, Last updated, Version 1) XX DE S.pombe chromosome I PCR product pJ735. XX KW conserved hypothetical protein; ubiquitin carboxyl terminal hydrolase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-1862 RA Saunders D., Quail M., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (03-APR-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9P7C5; Q9P7C5. DR SWISS-PROT; O14205; YDQD_SCHPO. DR SWISS-PROT; Q9P7C5; CWFG_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC PCR product pJ735 is overlapped at the 5' end by cosmid c9, EMBL CC entry SPAC9, accession number AL121764, and at the 3' end by cosmid CC c5D6, EMBL entry SPAC5D6, accession number Z98056. XX FH Key Location/Qualifiers FH FT source 1..1862 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /map="IL" FT misc_feature 1..119 FT /note="nominal overlap with SPAC9 S. pombe chromosome 1" FT CDS complement(1..404) FT /db_xref="GOA:Q9P7C5" FT /db_xref="SWISS-PROT:Q9P7C5" FT /label=SPAPJ735.01c FT /note="SPAPJ735.01c, SIMILARITY:Homo sapiens, O75270, FT hypothetical 36.6 kd protein, (316 aa), fasta scores: opt: FT 504, E():4.2e-27, (59.0% identity in 134 aa)" FT /partial FT /gene="SPAPJ735.01c" FT /gene="SPAC9.13c" FT /product="conserved hypothetical protein" FT /protein_id="CAB86262.1" FT /translation="MSERKVLNKYIPPDYDPSIRPPKKKKKFQGPNGGKLTVRLMTPFS FT MRCHTCGEYIYKGKKFNARKEKTGEKYFSIDILRFYIRCTRCAAEITFITDPKHADYAA FT ESGASRNYEPWHEKRLQEYEENELAERNDIP" FT CDS complement(1563..1862) FT /codon_start=1 FT /db_xref="SWISS-PROT:O14205" FT /label=SPAPJ735.02c FT /note="SPAPJ735.02c, len:>99" FT /partial FT /gene="SPAPJ735.02c" FT /gene="SPAC5D6.13" FT /product="putative ubiquitin carboxyl terminal hydrolase" FT /protein_id="CAB86263.1" FT /translation="SFRCLRTVALACSSYAANVLENALDNLNYEDREKAFSRVDELLCD FT FSQWPFDLHASSSVGANLPELISEELSSVKEEQLLFIEVVAAVLQVFTHLDSLL" FT misc_feature 1706..1862 FT /note="nominal overlap with SPAC5D6 S. pombe chromosome 1" XX SQ Sequence 1862 BP; 637 A; 313 C; 339 G; 573 T; 0 other; ggaatgtcat tgcgttcagc taactcattt tcttcatatt cttgtaatct tttttcatgc 60 cacggctcat aattcctgga agctccactc tctgctgcat aatctgcatg ttttggatcg 120 gttataaatg ttatttcagc agcacaccta gtgcagcgaa tataaaacct caatatatca 180 attgaaaaat atttttcgcc ggttttctcc ttcctagcgt taaacttttt tcctttatat 240 atatactctc cacaagtatg acagcgcatt gaaaatggag tcatcaaacg aacagtcaat 300 ttcccaccat ttggtccttg aaactttttc tttttcttag gaggacgaat ggaaggatca 360 taatcgggcg ggatatactt gttaagtacc tttcgttcag acattgttgg aaactatgaa 420 aaaaaaatga aatgaacgta cgagaattgc tttaccaaat tcaaaaaaaa agaataaatt 480 aaagtaagga aaatttaacc gtccttttgg gttggaatta tttgacttta cagatgaatt 540 tgaaaatgct agttttatgt ggatatgcgc tttttgtaag cgaatggcaa aagtactagt 600 actatctgta gttctttgac actcgatacg tcgcggtaat aaattgaatg cgcacatagc 660 actacgtaaa catatttagt accatcctta tatttcctta tacaatacct gagctgagct 720 atcgagacgt gagcaagcgt tgtttgtcaa tcttacacct tccggtgttt taagagtact 780 ttagattccc ttataaattg tgtattattt aaatgcttct gattaatttt gactgatcag 840 tctccatagt actttacgct tacagcaata gacaattaat ggggttgttg gcattaatgg 900 caggaataga tgatttatat gaacttggtt aaaaatcgct gtatttcagc ttatgttcgt 960 cttagtaaag ttgatagcaa tgatcataag aaaacacctg ctatattaat ttatcatatg 1020 ttcatttaag taaagcttgt ggaagaaccc aagtttaaaa tggtttaaaa gataactgta 1080 tagtaattac acaattgtca tttttcgtta gtttaataac taaagctttg tgaaaagaaa 1140 gactgactta tatgtacgta tgtaaatcat taaaacgaga agaaaatagc taacgatgtc 1200 cgcaactgaa aagaatgaaa gctgacccgg gatattctga acaaatcttt tttagcagac 1260 aatatcgaaa tgggattgcc tccatatcga gataaagtgt tcaactggag agagtatatg 1320 tggaaatggt accaccaatt taatgaaaat tcaactagat aatgaagcac cgattgttca 1380 aagtactatt cttacaacaa cgaacaattt gattgaattg acttctttta aaataatact 1440 ccaattaaaa acaaacaaat taagccgaca ttagcgatat agaagtcggt atacgtttgt 1500 tcattagtct gagttggaaa taacataact caaacaacac caacgagtga tatgcttggc 1560 tatcaaagta aagaatctaa atgagtgaaa acttgaagaa cagcagcgac tacctcaatg 1620 aaaagtagtt gctcttcctt cacagaggaa agttcttcgg aaattagctc aggcaagtta 1680 gcgccaactg acgaagaagc atgaagatca aaaggccatt gcgaaaagtc acagagaagc 1740 tcgtctacgc gagaaaatgc tttttcacga tcttcatagt ttaaattgtc aagagcgttt 1800 tcaagaacat tagcagcgta tgaggaacaa gcaagggcaa cagtgcgaag gcaacgaaaa 1860 ga 1862 //