ID   SPAPJ730   standard; DNA; FUN; 2271 BP.
XX
AC   AL139315;
XX
SV   AL139315.1
XX
DT   15-FEB-2000 (Rel. 62, Created)
DT   15-FEB-2000 (Rel. 62, Last updated, Version 1)
XX
DE   S.pombe chromosome I PCR product pJ730.
XX
KW   MAP kinase kinase kinase; win1.
XX
OS   Schizosaccharomyces pombe (fission yeast)
OC   Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes;
OC   Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
XX
RN   [1]
RP   1-2271
RA   Saunders D., Harris D., Wood V., Rajandream M.A., Barrell B.G.;
RT   ;
RL   Submitted (14-FEB-2000) to the EMBL/GenBank/DDBJ databases.
RL   European Schizosaccharomyces genome sequencing project, Sanger Centre, The
RL   Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail:
RL   barrell@sanger.ac.ukRL
XX
DR   GOA; O74304; O74304.
DR   SWISS-PROT; O74304; WIN1_SCHPO.
XX
CC   Notes:
CC   Details of yeast sequencing at the Sanger Centre are available on
CC   the World Wide Web.
CC   (URL, http://www.sanger.ac.uk/Projects/S_pombe/)
CC   During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced
CC   by the Sanger Centre.  The sequencing of the S. pombe genome is now
CC   being continued with funding from The European Commission.
CC   Fourteen European sequencing laboratories, including the Sanger Centre,
CC   are participating in the project.
CC   Protein coding regions (CDS) have been predicted with the help
CC   of computer analysis using the Genefinder program in PomBase
CC   (an ACEDB database) with additional predictions for the
CC   branch-acceptor sites supplied by the program Sp3splice.
CC   CAUTION: It is possible that for any individual CDS we may have
CC   underestimated or overestimated the number of introns/exons or
CC   we may not have chosen the correct splice donor/acceptor sites.
CC   CDS are numbered using the following system eg SPBC25H2.01c.
CC   SP (S. pombe), B (chromosome 2), c25H2 (cosmid name),
CC   .01 (first CDS), c (complementary strand).
CC   The more significant matches with motifs in the PROSITE
CC   database are also included but some of these may be fortuitous.
CC   The length in codons is given for each CDS.
CC   IMPORTANT: This sequence MAY NOT be the entire insert of
CC   the sequenced clone.  It may be shorter because we only
CC   sequence overlapping sections once, or longer, because we
CC   arrange for a small overlap between neighbouring submissions.
CC   PCR product pJ730 is overlapped at the 5' end by cosmid c1006,
CC   EMBL entry SPAC1006, accession number AL132828 and at the 3' end
CC   by cosmid c1250, EMBL entry SPAC1250, accession number AL110509.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2271
FT                   /chromosome="I"
FT                   /db_xref="taxon:4896"
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="972h-"
FT                   /map="IR"
FT   misc_feature    1..101
FT                   /note="nominal overlap with SPAC1006 S. pombe chromosome 1"
FT   CDS             1..2269
FT                   /codon_start=2
FT                   /db_xref="GOA:O74304"
FT                   /db_xref="SWISS-PROT:O74304"
FT                   /label=win1
FT                   /note="SPAPJ730.01, len:>756"
FT                   /partial
FT                   /gene="win1"
FT                   /gene="SPAPJ730.01"
FT                   /gene="SPAC1006.09"
FT                   /gene="SPAC1250.06c"
FT                   /product="MAP kinase kinase kinase Win1p"
FT                   /function="phosphorylates and activates Wis1 MAP kinase
FT                   kinase"
FT                   /protein_id="CAB75418.1"
FT                   /translation="VCPHIEHGFQIMSKSLSSLVGEILTNINRYLKDQLQGPDTDDSAL
FT                   ITSFYIKVLDCVRIRFRKLMSFTRILKAHLENSCEYVIKENSLSLLIQRLEESNHVLTY
FT                   TASIEHEGAYVIVPGHLVDSPNILREVLSMTFNKGDNNFESVPPYAVVLAPDSSICWNG
FT                   HVTDLDIPEVSISIAPNCVRLVTLATANQLSVIEDYFISIVGDTVSLVDSAKANSSKIN
FT                   KQMTKIKRNSLKLALSLLDVIQTIRTRYHGMNCQNLIHYSFSYAIEFAQRLMRLSILDA
FT                   SSIGLIRRKMIQLAISWVGFIYEDCSPTDRNTFRWTVTALEFAMIMTYGSNILMIDKKS
FT                   FEELKEKVGKCVALLLLHFDVMGTKHAGRSMDQQAGDIPARLVRNNSDRSRLSDNELAS
FT                   FVKEEVMHRIIELESNRRDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGG
FT                   LIGSGSFGTVYRAVNLDTGDLMAVKEVALHKPRISRPMIKRIKGEMLVLELFDHPNVVS
FT                   YYGIEVHREKVNIFMELCQGSSLFEFLRYGRIEDELVIQVYVLQLLEGLAYIHSCGVSH
FT                   QDVKPENILFDHNGIMKFTDFGSAKMSGSASTKIFEQLTQQEEEEFEKDSEFLQHLDQN
FT                   RGYSLTGTPTYMAPELILGNPSERVGAMDIWSLGCVIVEMATGSPPWPRLDNHFSLMYH
FT                   IAAHNPPIIPADDQLSPLGQNFLKRCFVSDPNQRATAAELLMDPWVYPLRAGTEFDLMN
FT                   SSV"
FT   misc_feature    1361..2221
FT                   /note="Match to PF00069 pkinase, Eukaryotic protein kinase
FT                   domain Score 255.99"
FT   misc_feature    2171..2271
FT                   /note="nominal overlap with SPAC1250 S. pombe chromosome 1"
XX
SQ   Sequence 2271 BP; 659 A; 413 C; 474 G; 725 T; 0 other;
     tgtttgtccc cacatcgaac acggattcca aattatgtct aaaagtcttt cttcgctggt        60
     tggtgaaata ttgaccaata tcaataggta tctaaaagat cagctgcagg ggcccgatac       120
     cgatgactct gcattgatca cctcatttta tatcaaggtg cttgactgtg ttagaattcg       180
     cttccgaaaa ttgatgagtt ttactcgtat actaaaagcg catttggaaa attcttgcga       240
     gtacgttatt aaagagaatt cattatccct tttaatacaa cgccttgaag aatcaaatca       300
     cgttttgaca tatacggcga gtatcgaaca tgaaggagca tacgtaattg ttccaggaca       360
     cctagttgat tcccctaata tacttcgtga ggtactttca atgactttta ataaaggaga       420
     taataatttt gaaagtgtac ctccatatgc ggttgtattg gctccagaca gctcaatatg       480
     ttggaacggc catgttacag atcttgatat tccggaagtg tcaatttcca ttgctcctaa       540
     ctgtgtccgt ttagtaacgt tagcgacagc taatcaactc tctgttatcg aagattattt       600
     tattagtata gttggtgaca cagtctcact tgttgattct gcgaaagcaa attcatccaa       660
     aatcaacaaa caaatgacca aaattaaacg aaattctttg aagttggctt tgtcattgct       720
     tgatgtgata caaacaattc gtacaaggta ccatggcatg aattgccaaa atctaattca       780
     ttattctttt tcttacgcca ttgaatttgc acaacgatta atgaggcttt cgatcctaga       840
     cgcatctagc attgggctga ttcgtcgcaa aatgatacag ctggcgatca gttgggttgg       900
     attcatctat gaggattgtt ctcctaccga tagaaatacg tttcgttgga ctgttacggc       960
     tttagaattc gcaatgatta tgacatacgg ttcgaacatt ttaatgattg ataaaaaatc      1020
     atttgaagaa ctcaaagaaa aggttggaaa atgtgtggca ttattacttt tgcactttga      1080
     tgttatgggt actaaacatg ctggtcgtag tatggaccaa caagctggag atataccagc      1140
     tcgccttgtt cgaaataatt ccgaccgaag tcgtttatcg gataatgagt tggcttcctt      1200
     tgtcaaggag gaggttatgc atcggataat tgaactagaa agtaatagaa gggaccgact      1260
     ttataagtcc cagctcattg gacgtgtttt ggatgatacg acaaaggaga acaggctatt      1320
     gaaagagcta gcctcttcga aatctaatat cacgattcga tggcaacagg gtggacttat      1380
     aggtagcggt tctttcggta cggtttatcg tgctgttaac ttggatacag gagatttaat      1440
     ggctgttaaa gaagtggcat tgcataaacc tcgtatatct agaccgatga tcaagaggat      1500
     taaaggtgaa atgttagtac ttgaactctt tgatcatcct aatgtcgtat cgtattatgg      1560
     tatcgaagtg catcgtgaaa aagttaatat atttatggaa ctttgtcaag gctcttcgct      1620
     tttcgaattt ctacgttacg gtagaattga ggacgaattg gtaattcaag tttatgtcct      1680
     acaattatta gaaggattgg cttacataca ctcctgtggt gtttcacatc aagatgtaaa      1740
     gcctgagaat atcctttttg atcacaacgg gatcatgaaa tttacagatt ttggttctgc      1800
     caagatgtcg ggttctgctt caactaaaat ttttgaacag ttaacacaac aagaagagga      1860
     agagtttgaa aaagactcgg aatttttgca gcatctagac caaaatcgcg gttactcctt      1920
     gacaggaaca cctacgtata tggcgccaga attaattctt ggtaatccgt ccgaaagggt      1980
     tggtgctatg gatatttggt cacttggatg tgttattgtt gaaatggcta cgggttcccc      2040
     accttggcct agattagata atcatttttc tttgatgtat cacattgctg cacataatcc      2100
     tcctattatt cctgcagatg atcagctttc gcctttgggt caaaatttcc tcaaacgttg      2160
     ttttgtttct gatcccaatc agcgcgctac agctgccgaa ttattaatgg atccatgggt      2220
     gtatccctta cgggctggta cggaattcga tcttatgaac tcatccgtag t               2271
//