ID SPAP32A8 standard; DNA; FUN; 8142 BP. XX AC AL583902; XX SV AL583902.1 XX DT 20-FEB-2001 (Rel. 66, Created) DT 20-FEB-2001 (Rel. 66, Last updated, Version 1) XX DE S.pombe chromosome I p1 p32A8. XX KW anaphase-promoting complex (APC); C3HC4 type RING finger; KW putative aldo/keto reductase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-8142 RA Saunders D., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (01-JAN-2001) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR SPTREMBL; Q9C1X4; Q9C1X4. DR SPTREMBL; Q9C1X5; Q9C1X5. DR SPTREMBL; Q9C1X6; Q9C1X6. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC p1 p32A8 is overlapped at the 5' end by cosmid c959, EMBL entry SPAC959, CC accession number AL357232, and at the 3' end by cosmid c3H5, EMBL entry CC SPAC3H5, accession number Z99296. XX FH Key Location/Qualifiers FH FT source 1..8142 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="p1 p32A8" FT /map="IL" FT misc_feature 1..101 FT /note="nominal overlap with SPAC959 S. pombe chromosome 1" FT CDS join(complement(1542..1721),complement(1376..1480), FT complement(991..1327),complement(887..946), FT complement(707..800),complement(1..656)) FT /db_xref="SPTREMBL:Q9C1X6" FT /label=apc5 FT /note="SPAP32A8.01c, len:, SIMILARITY:Arabidopsis thaliana, FT Q9SHK3, f12k11.7., (819 aa), fasta scores: opt: 266, FT E():2.5e-10, (23.2% identity in 340 aa)" FT /partial FT /gene="apc5" FT /gene="SPAP32A8.01c" FT /gene="SPAC959.09c" FT /product="putative Component of the anaphase-promoting FT complex (APC)" FT /protein_id="CAC29480.1" FT /translation="MVTPIFPIPLKTNSSKKNFYTTFLTPHKLTLCLLIELYAHRIIQP FT QHCVPFLDLVLGYFNPNDSQPQTLTRIHDITSHLPSSIEEYSVYSLLHERLWSLHSFED FT IHEIFISLGNYIEGVYDSEEEAPHMLFSSSSLLSIFLRKCVVEYEQFSFEQGVQFFKSF FT LQYRAPSMDFQHEGIEFCKINVRHELMNLASNKTLLQLAFGSIPVSRSSRDIEQLTQVQ FT IEHMQKFGCALPLEMKEKLHDLLEVEENNLNTSYYCKFLNSWFSGDYQQSVENLCRYFD FT HIMHSDEKVSYQYALLNLAMLQADFGCNEEALHAIEDTINTARESGDTACLNFALAWMF FT EFKRSQYSNPSPEKNENDDQILEHLARNSQKAQLPILQTTVHLLQVQKFLDDGSSLIGV FT FSNLTKAFALFCANESFDGFPRYMDLLQSIWYRLGCSILSMTYISIYQKNHVHDSPALD FT LLQSLITKAWRYAELGRWL" FT misc_feature complement(657..669) FT /note="ctaacatactaag, splice branch and acceptor" FT misc_feature complement(701..706) FT /note="gtaaga, splice donor sequence" FT misc_feature complement(801..813) FT /note="ctgatttacgcag, splice branch and acceptor" FT misc_feature complement(881..886) FT /note="gtaggt, splice donor sequence" FT misc_feature complement(947..959) FT /note="ctaacttttctag, splice branch and acceptor" FT misc_feature complement(985..990) FT /note="gtttgt, splice donor sequence" FT misc_feature complement(1328..1345) FT /note="ctaacaactttgttttag, splice branch and acceptor" FT misc_feature complement(1370..1375) FT /note="gtatga, splice donor sequence" FT misc_feature complement(1481..1498) FT /note="ctaactgtatatctctag, splice branch and acceptor" FT misc_feature complement(1536..1541) FT /note="gtaaga, splice donor sequence" FT CDS 3966..4817 FT /db_xref="SPTREMBL:Q9C1X5" FT /label=SPAP32A8.02 FT /note="SPAP32A8.02, len:284, SIMILARITY:Schizosaccharomyces FT pombe, O14088, putative oxidoreductase c2f3.05c, (275 aa), FT fasta scores: opt: 807, E():0, (45.2% identity in 272 aa)" FT /gene="SPAP32A8.02" FT /product="putative aldo/keto reductase" FT /protein_id="CAC29481.1" FT /translation="MSESQTESTTVTLTNGMVIPRIGFGAFMLKYNECYGLVTQALDSG FT YRHIDTAAVYGNEDICGKAIVDWCEKNNVKRTDIFLTSKLANCSDYYSTRAAIRSSLHH FT LGTYIDLFLIQSPAGGKKSRIASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKF FT YPCVNQIELHPFLSQDDIVKYCQSHDIAIEAYSPLTHGIRLNDEKLVPIAKKLNISVAQ FT LLIRWSLQKGYIPIIKSTKKEHMLSDLDVFNFTIPDDVVQELSSFDEHWHAGTTYDPTV FT CD" FT misc_feature 4002..4781 FT /note="Match to PF00248 aldo_ket_red, Aldo/keto reductase FT family Score 327.69" FT CDS join(complement(7178..7276),complement(5576..7018)) FT /db_xref="SPTREMBL:Q9C1X4" FT /label=SPAP32A8.03c FT /note="SPAP32A8.03c, len:514, SIMILARITY:Homo sapiens, FT Q9Y4L5, hypothetical 26.2 kda protein, (232 aa), fasta FT scores: opt: 393, E():4.6e-12, (36.1% identity in 158 aa)" FT /gene="SPAP32A8.03c" FT /product="C3HC4 type (RING finger) protein" FT /protein_id="CAC29482.1" FT /translation="MESQPVIWYCHSCSNEFQRPGNCPRCNSDFVEMVEPNTAPEDDPR FT AANFVNATEFNDPNAMLQNILQSLGQGVVPNVNPTDANRTANPNTNSNPNPNATNAQPN FT PTMFSTGQFSTEQGLPNVFFGAAAAQPGSGTPFVMPGIVNAAQIRNFFQNIMGGNAPQP FT EANSEQARNANTETSNPPFASAQTQGQEHRPSSPNPAEHMTGAYVNTPLNQPPSYAAST FT QPEFQQTTSPIFSSSSTPPPPPPRPSQPTSGESQNTNPRFPFSAQSFVFSVGPNGALHQ FT VNTSTENPQNAQAGAIPITDLGSILERIFGSLNQPGAQQGEGEPFNPANMFSNIFNLSG FT NPGDYAWGARGLDDIISQLMEQAQGHNAPAPAPEDVIAKMKVQKPPKELIDEEGECTIC FT MEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPVDPNSQQRNNTSTDSANGH FT NPSNHANPSTSTTNDQGATLRNESFNAASQSNLSSEHGHSSRTPMDDFVDEEPLE" FT misc_feature complement(5810..5932) FT /note="Match to PF00097 zf-C3HC4, Zinc finger, C3HC4 type FT (RING finger) Score 45.93" FT misc_feature complement(7019..7033) FT /note="ttaacatttgtgtag, splice branch and acceptor" FT misc_feature complement(7172..7177) FT /note="gtatgt, splice donor sequence" FT misc_feature 8041..8142 FT /note="nominal ovlreap with SPAC3H5 S. pombe chromosome 1" XX SQ Sequence 8142 BP; 2634 A; 1370 C; 1439 G; 2699 T; 0 other; ctaaccatct tcctaattca gcgtacctcc atgccttagt tataagactt tgtaataggt 60 cgagtgcagg agaatcatgg acgtgatttt tttgatagat cgaaatataa gtcatggaaa 120 ggatagagca tcctaaacga taccaaatgc tttgtagcaa gtccatatac cgagggaaac 180 catcgaatga ttcattagca caaaacaaag caaatgcctt agtcaaatta gagaaaacgc 240 caattaaact agatccatca tctaaaaatt tttggacctg taagagatga actgtcgtct 300 gtaatatagg cagttgagct ttttgactat tacgcgcaag atgttctagt atttgatcat 360 cgttttcatt tttttctgga gaaggattcg agtattgtga ccgcttaaac tcaaacatcc 420 aggccaatgc aaaattaaga caagcagtat cgccgctttc acgagcagta ttaatcgtat 480 cttcaatcgc atgaagagct tcttcattac agccaaaatc ggcttgaagc atggcaagat 540 tgaggagagc gtactgataa gaaacctttt catcagaatg cataatgtga tcaaaatatc 600 tgcaaagatt ttccacggat tgctgataat caccactaaa ccaggaattt aagaatctta 660 gtatgttagt taattgctag caggaaagat gtgcttggta tcttacttgc aataatagct 720 agtatttaga ttgttttctt caacttctaa taaatcatgc aatttttctt tcatttccaa 780 tggaagagca catccaaatt ctgcgtaaat cagttaataa gtgcttagat aatatgcatt 840 aaaaagctgt taatataatt gttaaaaatt ttccaatgaa acctactttg catgtgttca 900 atttgaactt gagtgagttg ttcaatatcc cgactagaac gagaaactag aaaagttagg 960 aaaaattaat ttcaaattaa ctaaacaaac ctggtatact tccaaatgcc aactgtagta 1020 gagtcttatt gctagccaaa ttcattaact catgacgaac attaatctta cagaattcaa 1080 tcccctcatg ttgaaaatcc attgagggag ctctgtattg aagaaatgat ttaaagaatt 1140 gaaccccttg ttcgaatgaa aactgctcgt attccacaac acactttcga aggaaaatag 1200 ataaaagtga agacgatgaa aaaagcatat ggggggcctc ttcttcagaa tcgtaaacac 1260 cttctatgta gtttccaaga gaaatgaata tttcatgaat atcttcaaaa gaatgcaagg 1320 accatagcta aaacaaagtt gttagaatac aatttttgat atgattcttt catacacgtt 1380 catgcaataa actgtaaacg ctatattctt caattgaact gggaaggtga cttgttatgt 1440 cgtgaattcg agtcagtgtt tgtggttgtg agtcattggg ctagagatat acagttagta 1500 ctcgagatta agagaatctt gtttcagtca gttgttctta cattaaaata tcccaatacc 1560 aaatctagga agggcacaca atgttgaggt tgaatgatgc gatgggcata aagttcaata 1620 agcaaacata gcgtcaattt atgtggtgtc aaaaaggtag tataaaaatt tttttttgaa 1680 gaatttgttt ttaaaggtat gggaaatatt ggtgttacca ttgacatcgc gggactctgc 1740 atggttaaat aggtaagcag tacacgatcg tctctcaatc ctataattta tagcaatgca 1800 ccaaattttc tcaatcacaa acagttcgaa tgttctatgt accaaataaa acctctttat 1860 cgaagaaggc tggaacagta atatgtaaat ggcatgtata cgttcagaag tgcgatggtt 1920 gagtattatg gtaagtagtc ttatgtttac aagatatgct aactattcaa gcatttaaat 1980 ctctagaagt aaacgtaaaa atttaatatt ttttcaagaa tgatatattt tctcggatat 2040 ttatttcttt caatttgtgt tgctcctatg ttcttcagtg atatttgtca acaatacgca 2100 taaacatatg aacttaccga aattgtgaat gatatataaa ctacctcaat atagttaata 2160 atcataatca tcaatatttt tattttattt aagcatcatt tttacagctt tatccacgat 2220 tgaaagtggt cttcataaat gttcgtaaac aaaaataaac aaacggacaa aaataataat 2280 aaaaagcttg agatatcaaa ttttataaga gattttcttt attctttatt ttctccttgt 2340 catctttatt gtttctatag ttcttttttt tttttttttt cgcaggcaca cacaaaaaat 2400 ttatttacta ttgggttaaa ttgagtaata atcatatgaa aagttttatt taaaaatcac 2460 tcacgtctct ataaactcat gttttgtttg ggtattaatt gtgaactgtg aagcattaat 2520 ttcaaaagat attcattatg atgcatttta aaaatatcat caatgcttaa aataatgcta 2580 tttaaataaa tccaataaaa tcgattaacc tttacctttt aaaagtgcat atttttattt 2640 atatggtttt ttaaaaatat gcataagtag gcatagaatt cgggtgtcat agctgatagt 2700 aatagagtaa ttgattgaaa tgatgaaata ttgcttttat tctttttttt tttttttttt 2760 tttttaatat acacaaagcc cttttaacat tttgacaatt atcaaattga tttgctgacc 2820 aacatttaat gacagaaagg taaataaata aattttggga agaaaaagaa agcaccattt 2880 cagttgtagt agactttcgt tttgtattac ctaaacgaat gcaataaaac tagctctcga 2940 agtttgtgaa taagaaatgt taattgggtt atatttgtct ataagtaagg taagttaaat 3000 tcaaaattgg aaatcaaaga aatacacaat cgaagtatgg ttaatctcta acgtatactc 3060 taaatgcact aaagtcccta ttgtaaatta aatgctaggc attttgatca agtaagcgaa 3120 cgatcgaagg aaaaaggata atcatacaag cgtgcaagtt caaatgatgt ctaagctacg 3180 cttaattcta ttcatttttt aaatttatta aaatattagc taaagttttt ctactaattc 3240 atcttacccc tctacacaac gcttgggtta aacagttaaa tcttcgacag gaagcctcgc 3300 tcatgaaaag cactttcaac ttgaaatgtg gacatccacg tggggatatc aataataaaa 3360 gtcagtttta acaacattca ttaatatttt tctattacac aagtaaatcg taagcactcg 3420 cttacttgta aaattttgaa accaaacttc tttttccgta gagaaaaagt tctgcacgtt 3480 tcctgaaacc atgaatgcag ctatccgttc cccttcaaaa tcattatcgc tgtcgggaat 3540 ttgtcaatat gctggttaat agatccccct tcgtagccgg atataaaaag cgatttgaac 3600 tctttcaatt tggcagcgta ttaccggatt gagggatccg aggacttagc gaattcatgt 3660 atatataaaa gaagggttcc tagtcaaatt ttattttaga agttaatgca atttcttgtt 3720 attttcagta tcatataata ataaagcacc attatattct ctaaaccaaa aataaaggat 3780 gcaaaaccat agacatcgtt aaattcccta aggcatttgt agctactagt tataccgatg 3840 ttgaagaact tcattaagga cgctaataag agaacctgac gtaatgtatc ctattgttat 3900 ttgcggcatg tgagctccaa gttacccaaa accaaagtat ttaggcaaag ttcaatatct 3960 tcaaaatgag tgaatctcaa acagaatcaa caactgtaac attaacaaat ggaatggtta 4020 taccaagaat tggtttcgga gctttcatgc tcaagtacaa cgagtgttac ggccttgtaa 4080 ctcaggcgtt ggattcaggg tatcgccata ttgatacggc tgccgtgtat ggaaacgaag 4140 acatatgtgg gaaggctata gtagactggt gcgaaaagaa taatgtcaaa agaactgata 4200 ttttcttaac ctcaaaactt gcaaactgta gtgactatta cagcactcgc gcggctatcc 4260 gtagtagcct tcatcatttg ggtacctata ttgacctttt cttaattcag tctcctgccg 4320 gaggtaagaa gtcgcgtatt gctagttgga aagctatgga ggagtttgtt gacagcggtg 4380 atatccgctc tgttggtgtt tccaactacg gtgtgaagca tttacaggaa ttatacgcat 4440 caaatcccaa gttttatcct tgcgtcaatc aaattgagct tcatccgttt ctttcccagg 4500 atgacattgt taagtattgc caatctcatg acattgccat agaagcgtat tcgcctttaa 4560 cccacggtat ccgtctaaat gatgaaaagc ttgtccctat cgctaaaaaa cttaacattt 4620 cggttgctca actgttgatc cgatggagtt tgcagaaggg gtatattccc atcattaaaa 4680 gcaccaagaa agagcatatg cttagcgatt tggatgtttt caactttaca attccagatg 4740 atgtcgtgca agagctatct agtttcgatg agcattggca cgctggtact acctacgatc 4800 ctactgtttg cgattagtct ttgtgttata tgtttctcta cgtttgttct ttttgtaatg 4860 cattagcagt attatactat gtttcgcaag tttacctttg ggtttttttt ttattaatta 4920 gttcctttat cagtgtgatg tgacgatgag atttagtacc aaagattttt tccctgcttt 4980 ataatcataa tctggacttt aatagtttta atgattacaa tcatcttgtt cctttttgat 5040 tttaattccc aattcaattg gtttttttta aaaaaattaa ttgagtatcc caactttttc 5100 atattatttt ttattaattc tttctctgat tattgttatc ataactgtta acaccttttt 5160 tgtttattca cctactggac cgtggttcct taattctcaa aaaaataatg aaaataccta 5220 ctacctgttc catgaatatc gctaccgacg aaccgtttgt ctaatttgca aaaaagctaa 5280 tttactaatt cattgtatgc ttttagccaa aataaatgat ataacatttt ataactataa 5340 tctaacaaaa atgaaaacag aagaagggcg tatcgtattc tcagatagaa aatcatacac 5400 aaacaacagt cctctaattc aaacccactt tgtgaataga aagaaaaccc gattatgcgt 5460 ctccttgtat ttcacaaaat aatgaaacga aaattaatgg ctctttttta ctcggaattc 5520 acaattataa ggtgttaaaa acttaaacgc atactacaaa ttattgagca cattactact 5580 ccaaaggctc ctcatcaacg aaatcatcca taggcgttcg acttgaatgc ccatgttcgc 5640 tcgacaagtt agactgagat gcggcattaa aagattcatt tcttaatgta gcaccttgat 5700 cgttagtagt gctagtacta ggattggcat gattagaggg attgtgccca ttggctgagt 5760 cagtcgaagt gttatttcgt tgctgactgt ttggatcaac aggagccctg caaattgcac 5820 aggtgccatt gactctcagc caaggtttaa tacaattttc gtgaaaataa tgcttacatg 5880 gaagctgtat aacatcgtca tttatcttaa acatctccat acaaatagta cactctcctt 5940 cctcatctat caattctttt gggggttttt gcaccttcat cttcgcaatc acatcctctg 6000 gagcaggtgc tggagcgtta tgtccttgag cttgttccat taactgggaa atgatatcat 6060 ctaggccacg agcaccccat gcatagtcac ctggatttcc ggaaagatta aaaatattgg 6120 agaacatatt agcaggatta aacggctcac cttcaccttg ttgagcgcca ggttggttga 6180 gcgagccaaa aattctttcg agaatagagc ccagatcagt gatagggatt gcaccggcct 6240 gagcattttg aggattttca gtggaggtat taacttgatg taaggcacca ttgggaccga 6300 cagaaaaaac aaaactttga gcagaaaaag gaaaacgagg gtttgtgttt tgagattcgc 6360 cagaagtggg ttgagaagga cgaggaggtg gtggtggtgg agtggacgag gaagagaaaa 6420 ttggggaggt cgtttgttga aattcgggtt gggtagatgc agcataggaa ggaggttgat 6480 tcaaaggtgt atttacatat gcaccagtca tatgttccgc agggtttgga ctgctaggtc 6540 gatgttcttg accttgagtt tgcgccgagg caaatggagg attgctagtt tcagtgttag 6600 catttcttgc ttgttcagaa tttgcttcag gctgaggagc atttccaccc attatatttt 6660 gaaaaaaatt tcgaatttgg gctgcattaa cgattccagg cataacaaaa ggcgtgccgc 6720 tgcccggttg agctgcagcc gctccaaaaa agacattggg taaaccttgc tcagttgaaa 6780 attgtcccgt agaaaacatt gtaggattag gctgggcatt tgtagcattc ggattcggat 6840 tcgaatttgt gttaggattt gcagttcggt tggcatcagt aggatttaca ttaggaacaa 6900 caccttggcc cagtgactga aggatatttt gtaacattgc atttggatcg ttaaattcag 6960 tcgcatttac aaaattcgca gctctcgggt catcttcggg tgccgtattc ggctcgacct 7020 acacaaatgt taacaaccag atcaatactt caaatgtctg ttaaggtatg caatagcatg 7080 attgttggtt atattgtgca taaaagtgtc tcttatacat aaataatgaa aaaataaatt 7140 acatctaaac agagaaagac ctctaaatac tacataccat ctctacaaaa tcactattac 7200 atcttgggca attccctggc ctttgaaatt cattcgagca agaatgacag taccaaatga 7260 ccggttggga ctccattctc ttgctcaaac gaccgagcta attcagttga cggcttctta 7320 ataaaaagct ggaaggtagt ggcgggtgat cgttcaagtt tcgtaaagaa gctatgttct 7380 tctatggtat agtaccacca tgcgtcgata atccaggttt atacacacta gtgttccacc 7440 aaaactacca tttattttta attaatttta aaatcttgaa taactttaga cgttgatcaa 7500 gtataataaa agcaatctac aaatattcct atttatgcat tggatatcgg ctatataaag 7560 ctcaaaatta taagataaat gtaatacacg caattaatca aaatacaatg atatccaaaa 7620 gttttgctgc actgtttata aaaatccagc cggcttttga atcatcttgc ttttttattt 7680 atttacttaa cagaatttcc gaaaggtaat tatttgacat ggtaaaagag agatgtgttg 7740 gaaatttcta aagataggaa gtagtcattt taacatatca gaagttaata tttttatgta 7800 ttttatttta tttttttctc aatgacatct ttcattatgg tttttctgta tttattatat 7860 ttttttaatt ttaaattttt tttttttttt gttccccata ttggtatgca gataaaggaa 7920 attatttcac acttcattta agctgcttta aaacagtcta aataaccctg gccatgtatt 7980 cctaacatat cataagtaag aatgtatatt aagtatttaa ctaaatttac ttcctctact 8040 gatctattta attatacata tgattcaaag gtgcaaaatg ctttaaagct actgcttcat 8100 tgacgaacaa ttatattcta cagcaaatat gtgtaaaatg ta 8142 //