ID SPACUNK12 standard; DNA; FUN; 6019 BP. XX AC AL157993; XX SV AL157993.1 XX DT 02-MAR-2000 (Rel. 62, Created) DT 07-FEB-2003 (Rel. 74, Last updated, Version 2) XX DE S.pombe chromosome I cosmid Unknown (NOT c25D11). XX KW calmodulin kinase i homolog; cmk1; G-beta repeat; trp-asp repeats; KW WD domain; WD40. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6019 RA Wood V., Rajandream M.A., Barrell B.G., Rieger M; RT ; RL Submitted (28-FEB-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Biotechnologische und molekularbiologische RL Forschung, Angelhofweg 39, D-69259 Wilhelmsfeld, Germany XX DR GOA; Q9P7I2; Q9P7I2. DR SWISS-PROT; Q9P7I2; KCC1_SCHPO. DR SWISS-PROT; Q9P7I3; YIX1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c25D11 is overlapped at its 5' end by cosmid SPAC664 CC EM:AL136235 and at its 3' end by cosmid c105. XX FH Key Location/Qualifiers FH FT source 1..6019 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c25D11" FT /map="IL" FT misc_feature 1..717 FT /note="nominal overlap with cosmid SPAC664 EM:AL136235 FT S.pombe chromosome I cosmid c664" FT CDS 450..2405 FT /db_xref="SWISS-PROT:Q9P7I3" FT /gene="SPAC664.15" FT /product="WD repeat protein; similar to S. cerevisiae FT YKR036C and YJL112W" FT /protein_id="CAB76232.1" FT /translation="MTDNNSKSKDSSQKIPNDSLTPTISKGLRKDVFYWTKAIAYTTWN FT FFDSSPVTPQRNPISLFVRGLSHPFLRHRLSKSQFNEWFLSHNGVDPVQAQTLPDPLLF FT DIPDHQETSYSLLEGYSVTAHDHSDTSPLPITSSNQKEGKKNVSSIDVSMVSDSSEFKP FT DSLQHEKLVKKCNQLRLQKLINSSELAQIDLELSKLYSRRRQVLERLSKIEEQNLKYTS FT KLASVEKLMLDSDAQDLYSSYSHDLIPSQIEAGKNGANPDVFDDTHDKYTDNLSARAIS FT PRAPRPSTASEVVSDYFEQNSAFSKAPENTESSVNQNYIVGNLVKQFQAHSEYITSLDF FT SHPFGTLATASTDKTVKVWDMAGVVYLGSLKGHSDYVSCLAIQDSFIATGSMDTTVRLW FT NLDNDVLHKDNPVEESLNSPPDQPVDNATNVLTSHTAPVTALALSSDDVLITGADDKTV FT RQWDIVTGRCIQTLDFVWAETHDTSTSQILVSDTYKQEPFIRALDCLDAAVASGTVDGL FT IRIWDLRIGLPVRSFIGHTAPISSLQFDSNHLYSGSYDNSVRIWDLRSGSPINIIPMEK FT KVNSLRLYQGRLAVASDEPNVRIFDTVSNRNWICSIPSHSDSIAAEPNSVPTSVQYKDR FT FLVDGKSDGSVSVFSA" FT misc_feature 1413..1529 FT /note="Match to PF00400 WD40, WD domain, G-beta repeat FT Score 49.83" FT misc_feature 1542..1649 FT /note="Match to PF00400 WD40, WD domain, G-beta repeat FT Score 47.67" FT misc_feature 1731..1835 FT /note="Match to PF00400 WD40, WD domain, G-beta repeat FT Score 39.90" FT misc_feature 2025..2135 FT /note="Match to PF00400 WD40, WD domain, G-beta repeat FT Score 44.83" FT 3'UTR complement(2910..3288) FT /note="mRNA from AF073893" FT CDS join(complement(4381..4544),complement(4279..4333), FT complement(4121..4211),complement(3718..4048), FT complement(3289..3655)) FT /db_xref="GOA:Q9P7I2" FT /db_xref="SWISS-PROT:Q9P7I2" FT /gene="cmk1" FT /gene="SPACUNK12.02c" FT /product="serine/threonine protein kinase; calmodulin FT kinase I homolog; CaM-dependent protein kinase (PMID FT 10617667); cell cycle-dependent expression (PMID 10617667); FT predicted regulatory site (Thr-192) (PMID 10617667); FT similar to S. cerevisiae CMK1 and CMK2" FT /protein_id="CAB76233.1" FT /translation="MQQTYKPNTSALKDGAPKATVDQKQLLPCKYRVGRVLGGGTYATV FT REAVHIETNKMYAAKIMNKKMMEKKQDFVKNEIAILKRVSYEHPNILHLVDFFETVNNL FT YLITELATGGELFDRICAKGSFYEADAAALMRTTTSAVKYLHDNGIVHRDLKPENLLYR FT SKDPNSDLLIADFGLSHFYEDSQYYMLMTACGTPEYMAPEVFRRTGYGKPVDMWAIGVI FT TYFLLSGYTPFARPSQVEVIEAILANEYTFNDPCWSGISETAKDFIKKCLENDPSKRLT FT AADALKHPFLSEKRPATSNLLPNVRENFNARKTFRTAYNAVRAFNTWKKLENKH" FT misc_feature join(complement(4121..4205),complement(3718..4048), FT complement(3424..3655)) FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 228.62" FT misc_feature complement(3656..3671) FT /note="ctaatactgtttatag, splice branch and acceptor" FT intron complement(3656..3717) FT /note="Intron confirmed by mRNA" FT misc_feature complement(3712..3717) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(4049..4064) FT /note="ctaacccttgaaacag, splice branch and acceptor" FT intron complement(4049..4120) FT /note="Intron confirmed by mRNA" FT misc_feature complement(4115..4120) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(4212..4234) FT /note="ctaacatgtttttggtggtatag, splice branch and acceptor" FT intron complement(4212..4278) FT /note="Intron confirmed by mRNA" FT misc_feature complement(4273..4278) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(4334..4351) FT /note="ctgaccaaattttcatag, splice branch and acceptor" FT intron complement(4334..4380) FT /note="Intron confirmed by mRNA" FT misc_feature complement(4375..4380) FT /note="gtaagt, splice donor sequence" FT 5'UTR complement(4545..4785) FT /note="mRNA from AF073893" FT misc_feature 5981..6019 FT /note="nominal overlap with cosmid SPAC105, EM:AL157994 FT S.pombe chromosome 1" XX SQ Sequence 6019 BP; 1949 A; 1078 C; 1038 G; 1954 T; 0 other; tggattcgct catccctcta gtacttacgt tgctttttcc ttccttcttc cacacaccag 60 tgacaaaaaa gcttgtatat tcaattgtct cttgattttt atcaaataca agctgagatt 120 aaaagagctg gacatttact acaaatttta gttgtttgct taattcccat tggtttttgt 180 tttgtaaaag tacgtgcggg aatcatactc ttattgaact tcttatacct tacacgtagc 240 gtcgattgaa aagattttat ttctcaactc atctaaaaat tgctttcttt tttaaggcct 300 tcatctggat atatcattta tctccgtaaa gccacctttt ttaaccgctt ttacgtttgg 360 gagatctgaa ccctttacct cattatttta attccttaaa gtattaaagt tcactagctt 420 ttcttctaat tgttggcttt tttcgtttta tgactgataa taattcaaag agcaaagatt 480 cttcacaaaa aattccaaat gattcgttaa ctcccactat ttcgaaaggt ttaagaaaag 540 atgtttttta ttggactaaa gccatagctt atacaacgtg gaatttcttc gattctagtc 600 cagttacacc tcaaagaaat cccatatctc tgttcgtgcg tggcctttct catccttttt 660 tacgccatcg tttatccaaa tcccaattta atgaatggtt tttatcacat aacggtgttg 720 atccagttca agctcaaaca ttgcccgatc ctcttttgtt tgatattccg gaccatcaag 780 aaacgtctta tagtttgctt gaaggttact cagtcactgc tcacgatcat tccgacactt 840 cacctttgcc gataacaagc tccaaccaaa aggaaggcaa aaaaaatgta tcatcgattg 900 atgtgtcaat ggtttccgac tcttctgaat ttaagcccga tagcttacaa cacgaaaaat 960 tggtgaagaa atgcaatcaa cttcgtcttc aaaagctcat taattctagc gaacttgcac 1020 agattgattt ggagttgtcc aaactctatt ctcgaagaag gcaagttctt gaacgcctta 1080 gcaagatcga ggaacagaat ttaaagtata cttccaagct ggcttctgtt gagaaactga 1140 tgttagattc ggatgctcaa gatttgtata gctcatattc tcatgattta attccttcac 1200 aaattgaagc gggtaaaaat ggagctaacc ccgatgtttt tgatgataca cacgacaagt 1260 atacagataa tttatctgct agggctatct ctccaagagc tccaaggcca tctacggcta 1320 gtgaagtggt atcagattac tttgaacaga attccgcttt ttctaaagca ccagagaata 1380 ctgaatcatc tgtgaatcaa aattatatag ttggaaatct cgtaaagcag tttcaagctc 1440 attctgaata tattacgagt cttgattttt cccatccttt tggaacttta gctacggcct 1500 cgactgacaa aactgtgaaa gtttgggata tggcgggtgt tgtatacctg ggttcattga 1560 aaggtcattc tgattatgtt tcttgtctgg cgattcaaga ctcctttatt gctactggct 1620 ctatggatac aaccgtgcgg ttatggaatt tagataatga tgtacttcat aaggataatc 1680 ctgttgaaga gagccttaat tctcctcccg atcaacctgt tgacaatgcc acaaatgttt 1740 taacatctca cactgcccct gtcactgcat tagcattatc tagtgatgat gtgcttataa 1800 ccggtgccga tgataaaact gttcgacagt gggatatcgt tacggggcgt tgtattcaaa 1860 ctttggactt tgtgtgggcg gaaactcatg atacatctac tagtcaaatt ttggtcagtg 1920 acacttacaa acaagaacct ttcatcagag ctttagactg cttagatgct gctgtggcta 1980 gtgggactgt cgatggactt attcgcatct gggatttgcg aatcggtctt cctgtgagaa 2040 gtttcattgg gcacacagct cctatttctt ctttgcaatt cgattccaac catttgtatt 2100 ccggaagcta tgacaattct gttagaatat gggatttacg aagtggctcg ccaattaata 2160 ttatacctat ggagaaaaaa gtgaattcct taagactgta tcaaggtcgt cttgctgtag 2220 catctgatga accaaatgtt aggatatttg atactgtatc taatcggaac tggatttgtt 2280 caattccttc tcactctgat tccatagcag ccgaacctaa ctcagtacct acttctgttc 2340 aatataaaga tcgctttttg gtggatggaa agtctgatgg atctgttagc gttttttctg 2400 cttaatttgt attttttatc cacaccttta tttattatac tctcgttatt gtgttgaatc 2460 tggataacgt taattttaaa attttatgtc tttatgcttt ggttaatggg tattaacttc 2520 tgtttggttt ttttatttat ttatggacaa ataaaaagga acgagacatt gcgaataaaa 2580 aactgtgatg ttaattttgt tcaaaatatt taaaaaaatt ctcaaagcaa attgtttgat 2640 caccacctcc cttctgcttc ttatactgta ggtttaaaat ttagtaaata aaaatctttt 2700 ctagtttaca tttttttggt tttactagta tgaagtacgc aacctcattc tgatctgaga 2760 tgttgtattt cattttgttg atccagttta caaaacacgc tcagcgtgtt taatgtcaac 2820 tacacataat aagtattgaa gaatcttttt gaaattagca atttgaactc acacagtgag 2880 taaaatatgg aaataaccaa gctcttttta tatttcagat taactgaatg gtcattttat 2940 ttttgaagca taaatagtca taaatgctaa aatttcaaaa ttttacatct gcaatatata 3000 tagagaatta aacaactgcg ttcatctgtc gtcaacagtc taacaatgtc agaaaactgg 3060 tagcgttact ttttaaaaca tcgacataac ttttagcatt ttgtatgagg ctattttaaa 3120 aactaagtag agaacatgat aggtccaagc attccaaatc acaaagaata tatttaacat 3180 gtaagagtgt taataccaaa aaagtatgta ctgagggcaa aagataataa agaagtcata 3240 agtcataaca agataaaata agaaagttat aagaaaagat gtgtataatt aatgcttatt 3300 ctcaagcttt ttccaagtgt tgaaagcacg tacagcgtta taggcagtac ggaacgtttt 3360 tcttgcattg aaattttccc ggacgtttgg aagcaaattg gaggttgcgg gacgcttttc 3420 agacaggaag gggtgcttca acgcatcagc agcagttaaa cgtttcgaag ggtcgttttc 3480 caaacatttt ttaataaagt ccttggccgt ttctgaaatg ccagaccagc aaggatcgtt 3540 aaaagtgtac tcgttagcta aaattgcttc tataacttca acctgagaag gtcgagcaaa 3600 tggagtataa ccactgagta aaaaataagt tataacgcca atagcccaca tatctctata 3660 aacagtatta gtcaagatac ataacttctg aattaaaatg gtagttgatc aacttacaca 3720 ggttttccat atccggttcg acgaaagact tcgggtgcca tgtactctgg tgtcccacat 3780 gccgtcatga gcatatagta ttgagaatcc tcataaaagt gggacaatcc aaaatcggca 3840 attaacaaat ctgaattcgg atctttagag cgataaagta aattttctgg cttcaaatct 3900 cgatgtacaa ttccattgtc atgcaagtac ttaactgctg atgtggtagt acgcattaaa 3960 gcagcagcat cggcctcata aaatgaacct ttggcacata ttctatcaaa aagttcacca 4020 ccggtggcca gttcagtgat taagtacact gtttcaaggg ttagtgaaca aaacacatca 4080 caataacatt tgattcatca aatcgaaatc ggagacatac gattattcac agtttcaaaa 4140 aaatcaacca agtgtaagat attaggatgt tcataagaaa cacgttttag aattgcaatt 4200 tcattcttca cctataccac caaaaacatg ttagtaaacg taaataagga tgctgtaagt 4260 acttaagtct ttacttacaa agtcttgttt cttctccatc atctttttat tcataatctt 4320 agccgcatac attctatgaa aatttggtca gcaaacaact cgaatttaaa atatacttac 4380 ttgttagttt cgatatgaac ggcttcacga accgtcgcat atgttccgcc tcccaaaacc 4440 cgaccgacac gatatttgca aggtaatagt tgtttctgat ccaccgtagc ctttggagca 4500 ccgtctttca aggccgaggt gtttggtttg tatgtttgct gcatagctgt aaagaatcaa 4560 tagatgaatt attccaatgc caacgaaggt aaacgtaaaa ggtttctctt aatgtaaatt 4620 attgtattgt tagatggtgc cataaacaat tctgtactac agaattcttt tatgaaacca 4680 aaaatgtaaa ggtatgtaaa tgaaaaaaat cccgtagaaa caataaagaa aatattattt 4740 gcgcgaagca ggctgttgag ttaaacggtg gttcaataac agaatgatct aagcagcgca 4800 atgactgtcc accaacttat gacaataagt gcttagcctc aacttgtaaa tatggaaaaa 4860 tttggaaaag gtatattgaa atcctcggat ttaacaggat attgatattg aatgtacccg 4920 aatagtattg atgaagataa caagaaaacc agcgtctgat aaataccgtc caactgattt 4980 gcaattgcaa aaccagtagt ctgcagtata gtcataaatc ctggtaattc ccaatcggtt 5040 cagtaatttc cgtacctatt ttcctcttct aagagaaaac aagatcagaa agatcaaaat 5100 tacaattagc gtattgaaac caccaaacac tgtaacaatg caacacaatt atttaaagaa 5160 ttgaatttaa ttatacaatc cttaaaaaat cgcagtttta tgagcaattg gcaaaacgcg 5220 aactgcttga aatttcgctg atattaccag ctcatgagag ttaagtagat ataacaagcc 5280 atcatggcga atattggtca ctaacgtata ccgtaataat gcgctatccc atgcatttta 5340 gtttgtttaa atacagtaag cgtattaaca acatagaaca gaatacgaaa aggtaaccac 5400 gtaacaaaga ttcatactgt aacaaaaatt gtgaaaaaca gactgagtgt aaattgagct 5460 aataaaatcg attttgaatg attactttta taatcttttc catcttggaa gaggtattta 5520 gttatttgag aaagaaccga actaccttct gaaaggaata ccatacattt tacaatagca 5580 gtgagtatgg tatgttaatt tctcatatgc ttcttgtatg atagttagtc gaaatgtatt 5640 ggagataatg ttgaagaagt aaatagtaaa aaatgcgcat gcatgcttga ctccatttgt 5700 tctacagtac tttacccttg ctatgatata aattcaaagt atcggtttaa acagacgaaa 5760 ccaataaggg tttaaactca catgggtttt tgaaggaaga tgagtctaca atgtaaacta 5820 ttcaactctt aaataacact ttttgataaa tcgcgatata gccaaaaact agaagaaccc 5880 taagtaactt cttcttatta aggtataaaa caagttttct acattgcgaa taacgaatag 5940 agatgaatgc attaattcta acattgaaaa taatatagtc gatcctttgg attccaaata 6000 aattatgaaa accatttaa 6019 //