ID SPAC959 standard; DNA; FUN; 15045 BP. XX AC AL357232; XX SV AL357232.1 XX DT 30-MAY-2000 (Rel. 63, Created) DT 30-MAY-2000 (Rel. 63, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c959. XX KW 40s ribosomal protein s4; 60s ribosomal protein l21; KW conserved hypothetical; G-beta; glycosyl hydrolase; invertase; KW mannosyltransferase; NSF attachment domain; rpl21-2; rps4-3; trp-asp; KW vesicular-fusion; WD-repeat; yeast sec17 homolog; yeast YER082C. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-15045 RA Saunders D., Harris D., Wood V., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (30-MAY-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O42706; O42706. DR GOA; Q9P4W9; Q9P4W9. DR GOA; Q9P4X1; Q9P4X1. DR GOA; Q9P4X2; Q9P4X2. DR GOA; Q9P4X4; Q9P4X4. DR GOA; Q9P4X5; Q9P4X5. DR SPTREMBL; Q9P4W7; Q9P4W7. DR SPTREMBL; Q9P4X0; Q9P4X0. DR SPTREMBL; Q9P4X1; Q9P4X1. DR SPTREMBL; Q9P4X2; Q9P4X2. DR SPTREMBL; Q9P4X3; Q9P4X3. DR SPTREMBL; Q9P4X5; Q9P4X5. DR SWISS-PROT; O42706; R21B_SCHPO. DR SWISS-PROT; Q9P4W9; RS4C_SCHPO. DR SWISS-PROT; Q9P4X4; SC17_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c959 is overlapped at the 5' end by cosmid c8E11, EMBL CC entry SPAC8E11, accession number AL021817. XX FH Key Location/Qualifiers FH FT source 1..15045 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c959" FT /map="IL" FT misc_feature 1..101 FT /note="Nominal overlap with SPAC8E11 S. pombe chromosome 1" FT CDS 1..268 FT /codon_start=2 FT /db_xref="GOA:Q9P4X5" FT /db_xref="SPTREMBL:Q9P4X5" FT /label=SPAC959.01 FT /note="SPAC959.01, len:88, SIMILARITY:Kluyveromyces FT lactis., Q9Y746, invertase, (609 aa), fasta scores: opt: FT 120, E():0.029, (34.0% identity in 50 aa)" FT /partial FT /gene="SPAC959.01" FT /gene="SPAC8E11.01c" FT /product="glycosyl hydrolase; invertase" FT /protein_id="CAB93008.1" FT /translation="SWKYPLYPERCTSSVPPSSYEDNYILEIEAVVDHSIIEVYLQGGI FT MCLTNAYYFKGDEPLQYYYLRVPTGASLAKSGMQPLLNNRPHS" FT mRNA 931..1129 FT /note="mRNA from spc05603 1 199" FT CDS join(936..968,1012..1129,1171..1474,1608..2022) FT /db_xref="GOA:Q9P4X4" FT /db_xref="SWISS-PROT:Q9P4X4" FT /label=SPAC959.02 FT /note="SPAC959.02, len:289, SIMILARITY:Saccharomyces FT cerevisiae, SC17_YEAST, vesicular-fusion protein sec17., FT (291 aa), fasta scores: opt: 778, E():0, (43.6% identity in FT 291 aa)" FT /gene="SPAC959.02" FT /product="putative vesicular-fusion protein yeast sec17 FT homolog" FT /protein_id="CAB93009.1" FT /translation="MIADPDQLMQKAAKKAQGTGGFALFGGGNKYDEASELFLDAANGY FT RLQKQGSAAGYAFEKAAEMQLKTDDKDDAASTYVEAFKSYRREKPSEAARVLQIAIELF FT TRRGNFRRAANYKMDLGDIFEQELQDTKAALGAYEDAGEWYSSDQADALANKAYLKAAD FT LAGLCGEYSLAIRKFEQVARASVQNNLLKWSVKDYLLKAGLCYMANGDEIATRRALEHF FT LEIDPSFASTREYQLLKDLQDTIEASDANMFADKVFTYDQLSKLDSWKTTILLKIKSSI FT QEAEDDLT" FT misc_feature 957..1799 FT /note="Pfam match to entry PF02071 NSF, NSF attachment FT protein, score 305.40, E-value 7e-88" FT misc_feature 969..974 FT /note="gtatgc, splice donor sequence" FT intron 969..1011 FT /note="intron confirmed by homology but mRNA is unprocessed FT for this intron" FT misc_feature 999..1011 FT /note="ttaacatctctag, splice branch and acceptor" FT misc_feature 1130..1135 FT /note="gtaatg, splice donor sequence" FT intron 1130..1170 FT /note="confirmed intron" FT misc_feature 1158..1170 FT /note="ctaacgtgtgtag, splice branch and acceptor" FT misc_feature 1475..1480 FT /note="gtatgt, splice donor sequence" FT misc_feature 1596..1607 FT /note="ctaacttgtcag, splice branch and acceptor" FT intron 2060..2266 FT /note="Intron predicted by HMM_intron, Score=17.52" FT CDS complement(2523..4085) FT /db_xref="SPTREMBL:Q9P4X3" FT /label=SPAC959.03c FT /note="SPAC959.03c, len:520, SIMILARITY:Mus musculus, FT Q9Z0H1, bing4., (622 aa), fasta scores: opt: 1277, E():0, FT (40.9% identity in 513 aa)" FT /gene="SPAC959.03c" FT /product="conserved hypothetical protein; similar to yeast FT YER082C; contains 1 WD-repeat" FT /protein_id="CAB93010.1" FT /translation="MSAQKLNLEDVNASTKKYSRGRKLNPKKIKDKKLRSNIQKIEERI FT ENVESSLAKTEILHEDNPGLLEAEGLERTYKFRQDQLAPNVALETATKSFSLDLDKFGG FT YSFDYTRDGRMILLGGRKGHISAFDWRTGKLLTELHLRETVRDVKWFHNHQYFAVAQKK FT YVYVYDNMGTEIHCLKRHIEVNALDFLPYHLLLTSIGNAGYLKYQDVSTGQLVAEHRTG FT MGASHVLHQNPHNAVEHVGHANGQVTLWSPSSTTPLVKMLTHRGPVRDLAVNRDGRYMV FT TAGADSLLKVWDLRTYKELHSYYTPTPAQRLTLSDRGLLAVGWGPHATIWKDALRTKQN FT FPYMNHLLPSSSVVDLHYCPYEDILGIGHAKGFESIIVPGSGEPNYDSYENDPFASRKQ FT RQETEVRQLLEKLRPEMISLNADFIGNVDRAAPSLRKAEAEEEKPPEEKWVPKAKARGK FT NSALRRYLRKHARNVVDQRRLKVEKSLEIEKKMRAQRVRREQKLPEEREKWGYALSRFV FT SKK" FT misc_feature complement(3207..3299) FT /note="Match to PF00400 WD40, WD domain, G-beta repeat FT Score 32.01" FT misc_feature complement(3579..3689) FT /note="Pfam match to entry PF00400 WD40, WD domain, G-beta FT repeat, score 10.50, E-value 2.3" FT CDS join(complement(6178..6644),complement(5820..6106), FT complement(5581..5723)) FT /db_xref="GOA:Q9P4X2" FT /db_xref="SPTREMBL:Q9P4X2" FT /label=SPAC959.04c FT /note="SPAC959.04c, len:298, SIMILARITY:Saccharomyces FT cerevisiae, YUR1_YEAST, probable mannosyltransferase yur1, FT (428 aa), fasta scores: opt: 275, E():7.2e-12, (24.6% FT identity in 321 aa)" FT /gene="SPAC959.04c" FT /product="probable mannosyltransferase" FT /protein_id="CAB93011.1" FT /translation="MYFLILFSSNAELGEVLRTMRSVEDRFNKRFHYPWTFYGMDEFTE FT YFMSTTSTIASGHCEYGTKGITYTNHLQFILTISYLVHWDLSQVKPLVENNRLYQQRAD FT ALAQENLSYVYSPLFHSFQDWVVRSLLYHPQLEYDYVWRIEPGLKLVCEEKKDIFSTFK FT DSDIVFTNHVCERKHSGIYAMEEAIEEYKILNTQGDFSNIWVYSNNYTYCKYWPFNEIL FT SLKQIRHNQTYTNLINYLLGSGGTYYHRWTESDILSAAFGVLRARANHMESVGFFLNDD FT VHYCPETIPYTGRCACL" FT misc_feature complement(5724..5738) FT /note="ctgacatttatttag, splice branch and acceptor" FT misc_feature complement(5814..5819) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(6107..6123) FT /note="ctaatatttttgttcag, splice branch and acceptor" FT misc_feature complement(6172..6177) FT /note="gtaaga, splice donor sequence" FT CDS join(complement(10078..10129),complement(8380..10022)) FT /db_xref="GOA:Q9P4X1" FT /db_xref="SPTREMBL:Q9P4X1" FT /label=SPAC959.05c FT /note="SPAC959.05c, len:564, possibly spliced to FT SPAC959.06c" FT /gene="SPAC959.05c" FT /product="hypothetical protein" FT /protein_id="CAB93012.1" FT /translation="MEFPDIRRAKVFCDLDLEFCAQQEIYDHPKVVVLKNGMWMRHVLE FT KNQITTKSARQFVKSHLGNCDLEQAENETDCFSDDGEYNSDSSSTDPAFELKEDQSWKH FT SSILRPLETLNFKRFLFGNEIMSKTRAFVMFVSLKHCEDCFHWEAVWSSITRNTDERLK FT MAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIKI FT EEGDIESIENSHPVFFLVLYDFATTSEDFSIIERLKLQLAGVAPLYICNSKALANKYGA FT QSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELTPTKCHSLLNRKL FT TVLTLLQPDSEQFFSSQEELLRLGKRWFRFQMQRQRNDIVWSRIKKYSAIAEAKKKGFA FT RKVKRIKYSKISHPTYTESVSFLWLDSSLWLDWIVENLDHTVYVDSITPPVFVIDHSKG FT VIYVSDRNGNSLTLEEDSLFSTLRIILEHPNSSRLQKLRAPGLCPNGSPNYRNRYKLIV FT FNLLIALLILSILTIISASRLSRRRRQLLNKQPVFGFYHSLVIAKSD" FT misc_feature complement(9445..9639) FT /note="Match to PF00085 thiored, Thioredoxin Score 25.69" FT misc_feature complement(10023..10034) FT /note="ttaacaatatag, splice branch and acceptor" FT misc_feature complement(10072..10077) FT /note="gtatgg, splice donor sequence" FT CDS complement(10719..11396) FT /db_xref="SPTREMBL:Q9P4X0" FT /label=SPAC959.06c FT /note="SPAC959.06c, len:225, possibly spliced to FT SPAC959.05c" FT /gene="SPAC959.06c" FT /product="hypothetical protein" FT /protein_id="CAB93013.1" FT /translation="MGENIEISTKVIENQEIRFRCYKADSTKIAVLAHPYAFLGGSVDD FT INIIALSKKINSKGFTVYVLDATTRRSALLSGKHDTMIFTLFVKYITSLNHPEYLLLGG FT YSYGARISMHKSITLAIDKRISHVSYLFLAPYLGLGSSILSWSWGLGFESFSTDSKVLF FT VWPDNDEFTREGTFETTLAKLKNRCPETTPLKLTDCSHMLSPSSRKILLETVDKWLASA FT LNV" FT CDS 11951..12739 FT /db_xref="GOA:Q9P4W9" FT /db_xref="SWISS-PROT:Q9P4W9" FT /label=rps4-3 FT /note="SPAC959.07, len:262" FT /gene="rps4-3" FT /gene="SPAC959.07" FT /product="40s ribosomal protein s4" FT /protein_id="CAB93014.1" FT /translation="MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLI FT VFLRNRLKYALNGREVKAILMQRLIKVDGKVRTDSTFPTGFMDVISVDKTGEHFRLVYD FT IKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLN FT LETNKIESFIKFDTSAQVMVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRL FT SNVFVIGETGKSWISLPKGKGVKLSITEERDRRRALKGLA" FT misc_feature 12119..12547 FT /note="Match to PF00900 Ribosomal_S4e, Ribosomal family S4e FT Score 291.14" FT CDS 13237..13719 FT /db_xref="GOA:O42706" FT /db_xref="SWISS-PROT:O42706" FT /label=rpl21-2 FT /note="SPAC959.08, len:160" FT /gene="rpl21-2" FT /gene="SPAC959.08" FT /product="60s ribosomal protein l21" FT /protein_id="CAB93015.1" FT /translation="MPHSYGIRARTRYTFQRGFREHGQIRLSTYLKTYKVGDIVDIKVN FT GAVQKGMPHKYYHGKTGVVYNVTQSSVGVLIYKVVGNRYMEKRVNVRIEHVKHSKCRQD FT FLDRVKANEAKRKEAKAQGKTVQLRRQPAPPATAHFVSTENNEPVTLHPVAYDTTI" FT misc_feature 13240..13536 FT /note="Match to PF01157 Ribosomal_L21e, Ribosomal protein FT L21e Score 207.10" FT CDS join(complement(14833..15045),complement(14656..14782), FT complement(14513..14608),complement(14166..14466), FT complement(13974..14119)) FT /codon_start=2 FT /db_xref="SPTREMBL:Q9P4W7" FT /label=SPAC959.09c FT /note="SPAC959.09c, len:219, SIMILARITY:Drosophila FT willistoni, Q9U6V8, polyprotein, (239 aa), fasta scores: FT opt: 105, E():1.4, (18.9% identity in 132 aa)" FT /partial FT /gene="SPAC959.09c" FT /product="hypothetical protein" FT /protein_id="CAB93016.1" FT /translation="IYQKNHVHDSPALDLLQSLITKAWRYAELGRWLDVATILSTAEVQ FT SQRSFQCAHLYGREKTFIYLSKAIDEKNHMDATLYLKNLNGFRHDKMSTFRTQLYALRL FT DISLGNLSQAIEGIDKLLEEPEKLAIEWVLQLMFLKVDIYMRTEMSNRAVFELLEIIQM FT ASSNYLRLYLLRGLCTLCLIGELDCFTAIDLLDSLIPDIIAADNKPLLALAFHAFASLQ FT IKHLDLLQPSRMFVIQLLDSAIDGYRACYMVEMQKTVVRLKIQYLRQINDPALQPNIEQ FT YCSRFYSDNLLN" FT misc_feature complement(14120..14134) FT /note="ctaatgattaaatag, splice branch and acceptor" FT misc_feature complement(14160..14165) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(14467..14483) FT /note="ctaatgtgagtgtttag, splice branch and acceptor" FT misc_feature complement(14507..14512) FT /note="gtaagg, splice donor sequence" FT misc_feature complement(14609..14624) FT /note="ctaaacgtgttactag, splice branch and acceptor" FT misc_feature complement(14650..14655) FT /note="gtaatt, splice donor sequence" FT misc_feature complement(14783..14796) FT /note="ctaattttgaaaag, splice branch and acceptor" FT misc_feature complement(14827..14832) FT /note="gtaaga, splice donor sequence" XX SQ Sequence 15045 BP; 4769 A; 2757 C; 2688 G; 4831 T; 0 other; tagttggaaa tatcctttat atcctgaacg gtgtacgtct tctgttcctc cctcttctta 60 cgaagataat tatatattgg aaattgaagc tgtagtagat cactccatta ttgaagttta 120 tttgcaagga ggaataatgt gcttaacaaa cgcctactat tttaagggag atgaaccatt 180 gcagtattac taccttcgtg tcccgactgg agcttcttta gctaaatcag gcatgcaacc 240 attattaaac aacagaccgc attcctaggt tccaaatgaa tcaggcaata cgaaatcgat 300 acagtttctt ccgtcagcgt tttctcttat tttctactgt gtattgatga aaatccaagt 360 atgttatgaa tacacgtggc tagtaaattc cgaaaattac tcgggatttt cgatacccat 420 cttccaaact atttctcatc taaatggata agatatagtt tccaaaaatc cacacgttat 480 ccactattgt tcgttgttta tttctgtttt tgtttttgtt acaatttgat tccgttccat 540 ttctttacag ttgtcgttat tctgcgtcga ttccgacgcc aagtttttaa acacgactgg 600 agatgtatat accaaatatt tatttttaac agctctatgt ttgttagtct gacttgtaaa 660 ttactataaa aaaaagaaac attttattac gaattgctcc tatgctttcc cattcttgag 720 taaaaagttc ccaatttcaa ttctatcctc cgttgacaag gtgaaaaaga aaaattgatg 780 aactgaagaa aacagattta gttagtctaa aaacatattt ttaaatatga ttgctgtcgt 840 atttacagta taacttaata ccctaatcaa taaaggcata taacaccata agtaacacat 900 agcatgagtt caacgtacca ataaccatac tttccatgat cgcagaccct gatcaattaa 960 tgcaaaaagt atgcaagaag ttgcggaaat atagttgatt aacatctcta ggccgccaaa 1020 aaggcccaag gaactggtgg ctttgcgctt tttggaggag gaaacaaata cgatgaagct 1080 tcagaattat tcttagacgc tgctaatggt tacagactgc aaaagcaagg taatgatttt 1140 gatttacttt tcaattacta acgtgtgtag gatctgctgc aggttatgct tttgaaaagg 1200 ctgctgagat gcaattgaaa acggatgata aggatgacgc tgcaagtact tatgtggaag 1260 cttttaaatc ttatcgacgc gaaaagcctt cagaagcagc tcgtgtacta caaattgcca 1320 tcgagttgtt tacaagacgt ggtaacttcc gccgtgctgc taattacaaa atggacttgg 1380 gagacatttt tgagcaagag ctccaagaca caaaggctgc cctgggagct tatgaagatg 1440 ctggagaatg gtattctagt gatcaagcag atgcgtatgt agtttacctt tttttcgtat 1500 acagctgacg acctaaacaa agtgacagat tcactttatt tgactcgtct aaattttatt 1560 attattattt ttttctaatt aattatcttt tacaactaac ttgtcagact tgcgaataag 1620 gcttacctta aagcggccga cctagctggc ctttgcggtg aatattcttt agctatacgg 1680 aaatttgagc aagttgccag agcaagtgtt caaaacaatt tgctaaaatg gagcgttaag 1740 gattatttgc tgaaggccgg gttgtgttat atggctaatg gggatgaaat tgctactcgt 1800 agagctttgg aacacttttt agaaattgac ccgtcttttg cctccacccg tgaatatcag 1860 ttgcttaaag atttacaaga taccatcgaa gcctcggatg ctaacatgtt cgctgacaaa 1920 gtttttacct atgatcaatt gtcgaagttg gattcttgga aaactacgat tttactgaag 1980 attaagagca gtatacaaga agctgaagat gacttaactt agttttgttt ggtttaagtt 2040 ttcaatcacg tgccttgaag taagtgaaaa aacactttca ttggcttttc gaatgcatgg 2100 gttttccaat gtcagtaacg ttcgtattca tatttgtttt tttttaagaa ccttttttac 2160 attatataac atttttattt aaattgtttt ttattttgtg gaattaagct atatatatgg 2220 tcaacttgac gagaatgatt gcaacttttg tttgctaata ttataggatt tactggtttg 2280 atctttcaac ttgttgtcta caaatggaaa caaagagagg gttaggatga tccttgtttt 2340 acatttatat atgcttgcac aatgtacaat ttttcaaata ggcattttat tttttttgtg 2400 agacatattt aatacaataa atccgtaacc ctaaatacct tatacatcac atagtcatat 2460 gtaattggca aaatctaatg caaatattct tattattagc ccaagtatta tacacaaccg 2520 aattattttt tggaaacaaa tcttgataaa gcatatcccc atttttcccg ttcctctgga 2580 agtttttgct cgcgacgaac acgctgagca cgcatttttt tctctatctc taaagatttt 2640 tcaactttga gacgacgttg gtcaacaacg tttctggcat gtttgcgtaa atatcttcgt 2700 aaagcagagt ttttaccacg ggcttttgcc tttggtaccc atttttcttc aggcggcttt 2760 tcttcctcgg cttcagcctt tcgaagcgac ggtgcagctc tatcaacatt accgataaaa 2820 tcagcattca aagaaatcat ttctgggcga agcttctcca aaagttggcg aacttcagtt 2880 tcttgacgtt gttttcgaga agcaaaagga tcgttttcat atgaatcata attgggttca 2940 ccagatccag gaacaatgat gctttcgaat ccctttgcat gtccgatacc taaaatatcc 3000 tcatacggac aataatgaag atcaacaacg gatgaagagg gaagcagatg attcatgtat 3060 ggaaaatttt gtttagttcg taaggcatct ttccaaatcg tagcatgtgg tccccatcca 3120 acggctagca atccccggtc actgagggtt aagcgctgag caggtgtagg ggtgtaatag 3180 gagtgaagct ctttgtaagt tcgaaggtcc caaactttca gtaaactatc ggcaccagcg 3240 gtaaccatat atctgccatc acgatttact gccaaatcac gtacaggccc gcgatgagta 3300 agcattttga caagaggtgt agttgaagat ggagaccaga gggtaacctg gccatttgca 3360 tgaccaacat gttcaacagc gttatgggga ttttgatgta atacatggct agcacccatt 3420 ccggtacggt gttctgcaac caattgtcca gtagagacat cttggtattt caaatagcca 3480 gcattgccaa tactggtcaa tagtaagtga tatggcaaaa aatctagagc attcacttcg 3540 atatgccgct tcaaacagtg aatttctgtt cccatattat cgtaaacgta aacatacttc 3600 ttttgtgcaa cagcaaaata ttgatggtta tgaaaccatt taacatctcg aacggtctct 3660 cttaaatgta gctctgtgag taatttacca gtacgccagt caaacgcgga aatatgacct 3720 tttcttccac ctaataaaat cattcttcca tctcttgtat aatcaaatga gtaaccacca 3780 aacttatcca aatccaagga gaaagacttt gtagccgtct caagtgcaac attaggagcc 3840 agttgatctt gacgaaactt gtatgtcctt tcaaggccct cggcttccaa aagaccggga 3900 ttatcttcat gtaagatttc ggttttcgcc aaggaagact caacattctc aattctctcc 3960 tcgattttct gaatattgct tcgaagcttt ttatccttga ttttctttgg attcagcttg 4020 cgccctctag aatatttttt tgtagaagca ttaacatcct ccaaattcaa cttttgagca 4080 gacataatgc taactaagta aagatattag tgaggaaaaa agattttttt ttctttttag 4140 gcctgattat acaaaaaaaa tgaagcagtt ttctaataaa caattctttc caaaatttag 4200 gtatctccct tagtcaaaaa gttagaaacc cttcaatttt tacacaaatg tattatgtaa 4260 acgaagtgat agttaaaaaa ttcaaacact acgttcgact tcgattctat gacccaacag 4320 taggtttagg ttgagggcag gtggaatgta gttctaggaa taggatttgt gcgttcttat 4380 tgtaaaaaat cagaaactac tactaacaca attagataca aaccacaatt atttacaaat 4440 aatacaagta taataaaata aaatttcata aaaattaagt atgcaaaata aaaacaagaa 4500 aattttaaaa gtaaacccga aacccagtaa aaacattata agttcagaca atggccaatg 4560 ccaaaactgc gaaatgaacc atgaaacgaa aatagctcag gaattaattc tcctgctttt 4620 tggtcatgga aagaagctgg atcgaattgg atatatcgct catcttcgaa ccgctattat 4680 gagcacttct tagtacgctc tgcgtagtgt tgtaatcatc aatactctgt aaaaggacgt 4740 tttgttggta tttgttgttt ttagaggagt ttttgttttg ttttgaggat ttcttaactc 4800 cttttgaagt ctgtttaggc ttttttgact gcttgttcct tttctgtgcc atttggttga 4860 agttaaaagt tttatcacgg aggcgataat tcattttttc aacaaaattt tccttctcaa 4920 aagtaaagat atatttatta taaacaaata cacatgcaac ataatacatt tctccctaac 4980 gatgctgtag aagagcaaaa gcaaaatcca tactatggaa tactgagagc agtggaaacc 5040 gacttaaggc tcggtcaacg ttggtgtgaa ttaaaggtac atgttcttga tattgactta 5100 aaaacaaaaa ggcctttact ctcagggatt cccgtaaatg gacagctcaa agacaaattg 5160 caatatgttc tgccgcttta cctccaagag acaatttcga tcgagtttct gtcctctgtt 5220 ttcgattcta tgaagaaact gagccttcca cttgtaaagg atgctcgagg tctttctgga 5280 gatgagtttt tcctatacct gggaatcatg tgttctgact caacgatagt atattacaag 5340 atcaccgatg gacttataaa accaaggcaa aatgatgaag aataaacaca tgatatatac 5400 atatttattg attaggattg aaaaacggaa agacaacgct ataattttac cactaccaga 5460 ttaatttttc acttcaagtg tctcaacgtt tgtaaatgaa aatgcacata ttacaataac 5520 tttcataaaa caaattcgta tgaacaaaaa ttttctgtaa agctcattca tcaattaata 5580 tcacaaacat gcacatcttc ctgtgtatgg aatagtttct gggcaataat gaacatcatc 5640 gtttaagaaa aatcccactg attccatatg attagcacgc gctcttaaaa caccaaatgc 5700 tgctgaaagg atatcagact cggctaaata aatgtcagca aaaagtacaa cgtgaagcat 5760 ttatattaga aacaggttaa ataaagtcat ttccaaaatt gaagaagcaa tttacttact 5820 ccagcgatga taatatgttc ctcctgaacc aagcaagtaa ttaattaggt tggtgtaggt 5880 ctgattatgc cgaatttgct ttagagataa aatttcatta aaaggccagt atttacaata 5940 ggtgtagttg ttactataaa cccagatatt gctgaaatct ccttgagtgt ttaaaatttt 6000 gtattcttca attgcctctt ccatagcata tataccagag tgtttacgtt cacagacgtg 6060 gtttgtaaat acaatgtcgc tgtctttgaa agtagaaaat atatctctga acaaaaatat 6120 tagttaggtt tcctgaaaaa gaagaacgaa ataaacaagt tttaccgagt ttcttacttt 6180 ttttcttcac aaaccaattt gagcccgggt tctattctcc aaacataatc gtattccaat 6240 tgaggatgat ataataaaga tcgaactacc caatcttgaa aactgtgaaa caaaggagag 6300 tatacgtaag atagattctc ttgagcaagt gcatctgctc gttgttgata aagtcgattg 6360 ttttcaacta ggggttttac ttgagaaaga tcccaatgca ctaagtaaga gatagttaga 6420 ataaattgta aatgattggt ataagttatt cccttcgtac cgtattcaca atgaccagac 6480 gcaatcgtgc ttgtcgtact cataaagtat tccgtaaact catccatgcc gtaaaacgtc 6540 caaggataat gaaacctctt attaaatcta tcctctacgc tacgcatcgt gcgcaacact 6600 tccccaagtt cagcattact agaaaacaga attagaaagt acatctcaaa tggcaccgta 6660 cgcggtaaac atcagaaaag aagcactcaa attggagctt tgaggttcat taagaatcac 6720 atatacattg ttgggttcat ttacagagaa tccattcaac caggtaaata caattggtag 6780 tatggaaagt aaaagttcgt aaaacataat gaagcttaaa cacgagcaga actttccctg 6840 ttatgagaag taactagttc aactaacgta tgactccaaa agtaacgcat agaaagtgga 6900 tttgaaggaa tttaactgaa attgcctaag acaatggtta agggcaaatg ttaataccct 6960 gctagagatt tgaccttaga aaagagagct ctttgagtat aaatatttat ttttagcaaa 7020 tcaatttttg tatacaatgt ttgatgggta gatcactgaa agaatctgct aacgagcata 7080 atcttataaa tgacatttgc ataagcgtac tgatagtcgc tatgtcgagc tgttattact 7140 gttggaatat ttaagttcta tcaaaggaat tataaaattt gcattcgtct tttataatgg 7200 tttttaatgt ctattaaaca attcattgtt gctgatgaat gtaaacaaaa gcaataagtt 7260 taagtatagg aaagcttcag ctgttctaga atacaaactc agtaaaatgt tataaaataa 7320 tatttcataa acccaagtgg catatgtatc ttaagacatt ttcccaatat ctgcaaacta 7380 attcactact ttcgatcaaa ttatatactg tggttgttca taatgcacaa tcttatttta 7440 acgagccttt ttttttgctt tctggaaaca ccaaaattcc tagactacta agggtaaaac 7500 agtcactagg gttagggttg taattttcaa caaactatat aaagtctcgt ttgcacttcg 7560 ttataaccta tcatgaatct gaagttacat tgttcagatt ctttcgagaa tcttgcgtgt 7620 aacatggttg gcgtgtagag gttgacaaga gtacagacag ggcggcgatt gtccttgcag 7680 tttcgactgc aggttgagag ttggcgtctt gccatgaaaa ttgactaaag gatgctgcgt 7740 agcctagcca acagattcta aaacgattgg agtgatcctg atccttctcc tttggagagg 7800 aattggttga tcctctgaca actgtatatt cctaaatctt tcgctacatg atctctggat 7860 ccttgcagtt gggtaaaacc atacagttga catttttcat ttgggtagtt cttcttttat 7920 gatagtttaa tcatgtgtat gaatacgatt aattatttta ttacaatttt actttgtcat 7980 ttaaaactga attttttaaa cttccaattc gctgttaatg aacaaataaa aatgggcgtt 8040 gtgatgtttt gatgagaaac aggctgacct gtcgatgtag ttttcgtttg tacagcttga 8100 ccgtttgagg taggttgcca gtatggtatt ttgtatactt aaactgaatt tttaatgata 8160 atattctcgt catattttga ggtactttgt tttcgtgtat aagtccttgt caaattaaat 8220 ggactacaaa gtttttaaca atcagttttg tgggctgttt gtagcaacat cacaagtgta 8280 ttagcaataa atgatttaga aatgctaccc taagagagta tataaaaaaa acctttcgct 8340 ccttttcatc gtttactagg tcgttgaatt gtcaacattt cagtctgatt tggcaattac 8400 aagcgaatga taaaaaccaa aaactggttg cttgttcagc agttgtcgac gccgacggga 8460 aagcctgcta gctgaaataa ttgtcaggat gctcaatatt agtaaagcaa tgagcaaatt 8520 aaaaactata agtttgtatc tattcctgta attggggcta ccgtttggac ataggcccgg 8580 tgcacgcagt ttttgaagac gagatgagtt agggtgctct aatataatcc ttaatgtgga 8640 aaatagagaa tcttcttcta acgtaagtga gttgccgttt ctgtcactca catagattac 8700 gcctttcgag tgatctatca caaacactgg tggtgtgata gaatctacat aaacagtatg 8760 gtccaaattt tccacaatcc aatccaacca taaagaagaa tctaaccata aaaatgaaac 8820 tgactcagta taagtaggat gtgatatctt cgagtactta attcgcttca ctttcctcgc 8880 gaaccctttc tttttagctt cagctatcgc actatatttc ttaattctac tccaaactat 8940 atcatttctt tgtcgttgca tttgaaatcg aaaccatctc tttccaagcc gcaacaattc 9000 ttcttgactt gaaaaaaact gttcagaatc tggctgaagt aaggtcaaaa cagtaagctt 9060 tcgattcagc agactgtggc attttgtcgg ggttagctct gtaataaaag gggaggaaac 9120 tatgttaatc cactcagtaa ttcgtttata atctcgaaac tcacggggtg taattgcctg 9180 ataaactatt ggcattccat tgcgaacagc aataatgcta ggctgagact gtgccccata 9240 tttattcgcg agcgccttcg aattacaaat gtataatgga gcaacaccag caagctgtaa 9300 tttcagacgt tcaatgatac tgaaatcttc tgaagtagtt gcaaaatcgt aaagcaccag 9360 aaagaaaact gggtgagagt tttcgattga ttcaatgtcc ccttcttcga tctttattgc 9420 ttggtaactt gccacttggt ttgaatagga tagcagctgt tgatacttga gaggtccatt 9480 atattgtatg gaatcgaacc cctgaaacac cctgaaggta ggaaactttt taatatggaa 9540 atgattacac atctcctttt cttcgtcgca attaacttgc gccattttta atcgctcgtc 9600 ggtattacga gtaatgcttg accaaacagc ttcccaatga aagcaatctt cacagtgctt 9660 caatgaaaca aacataacga atgcacgagt tttagacata atttcatttc caaaaagaaa 9720 ccttttaaag ttcaatgtct ccaaaggcct caaaatactc gagtgtttcc acgattggtc 9780 ttctttcagt tcaaaagcag gatctgtgct actggaatca ctattatact caccatcgtc 9840 actgaaacaa tctgtttcat tttcagcttg ctccaaatca caattaccca aatggctttt 9900 tacaaattgt cgcgcggatt ttgtcgtaat ttgatttttt tcaagaacgt gtcgcatcca 9960 cataccattt tttagaacta ctacctttgg atgatcatat atttcctgtt gagcgcaaaa 10020 ttctatattg ttaataatta gtaaaaactt taattcttat gatgtatcta tccataccaa 10080 ggtctaaatc acaaaacacc tttgcacggc gtatgtcagg aaactccatt gaaacttcat 10140 tccaaataac agaaaactct tccttatcat atttgctttc agtgaactct acaaaccaaa 10200 atgcagtagt attcaagatg gacgaagcat tgtgcgaact aacgataatc tcctgcttaa 10260 ttgaaaaagc gtaactaaaa ctaataataa agtagtagac tatgaaagtg aaatgataaa 10320 ggaagagctt catttccctt tttcgagaca atggttggga gtttccaatg tttactaaat 10380 catgcagaga atgtgtagca tacaaatgaa aacgtaaaca aacattttca aacagaaggg 10440 ggctattttg acacaatcaa taattctttt taataattta tctttttgca aattagtctt 10500 cgtgaatgtg ataataaaga actttttctc acatgaggaa aagccggcta gataagaaca 10560 gtgaaatcct tagaagcaaa agaaaaagga atctaaatga cgaacacgtt aatatattta 10620 aacaactccc cttgagttat ggaaaaggct tttctctaga gctgactcgg taatattttt 10680 tttagtaatt tacaattgac attcaaattt atcagatgtc acacattgag ggcagaagct 10740 aaccatttgt ccacagtttc taaaagaatc tttcttgagg atggtgaaag catgtgggag 10800 caatctgtaa gttttaaagg agtagtttct gggcaacgat tttttagttt tgccagcgtc 10860 gtttcaaagg ttccctctct agtaaactca tcattatcag gccaaacaaa caatactttt 10920 gaatcagtag aaaaagattc aaagcctagc ccccaagacc aacttaaaat actgcttcca 10980 agtcccaaat acggtgcaag aaaaagatat gagacatgag aaatgcgctt atcaattgct 11040 agggttatag atttatgcat actgattctt gcaccgtaag aatatcctcc caacaaaaga 11100 tattccggat gattcaaact agttatatac ttcacaaaca aggtaaatat catcgtatca 11160 tgtttaccac tcagcaaagc agaacgtcta gttgtagcat caaggacgta aacagtgaat 11220 cctttggaat ttattttttt tgacaaggca atgatattga tgtcgtcaac ggagccacca 11280 agaaaagcat agggatgagc caaaactgcg attttggtag agtcagcttt gtaacatcga 11340 aaacgaattt cttgattttc tatgacttta gtcgatattt caatgttttc acccatagaa 11400 tatctattcg ggaattttac taatcggtga actacgttcg ttagtaaaac taaaaacttg 11460 attgaaactt caaattttca aatagtttac tgaatacaga gcttatttag cttactgctc 11520 gaaattaata attccaaacg tcaaactgat tttaataagt agtaatgatg aagatgttag 11580 ttgtatatga tgacaacata aataaaacaa aatttacaag aaattaatca gtgtcacaac 11640 ataaaatatg ctttaagata tcaaagaagt tggccaaata taaatagaac ttttaaaatg 11700 ccataaaagt catagaaact acttcttata ccaacaaatt ggcacatcac aaaagtacat 11760 gtttgtatag tcataaaaca acaaaataat gaaacagtat ttgataaatt caattaaaaa 11820 ttatattttt atggcattta aagtcatttg gctcatgttt cttgcttttg ttttgttgac 11880 gaaaaaggca gtcacaatta tttgcttact gtagcagagc aggaaatgaa ccttcaacca 11940 cgacaatacc atggttcgcg gaccgaaaaa gcatctgaag cgtgttgctg ctcctcacca 12000 ttggctttta gacaagctct ctggtactta tgcccccaag ccatcacccg gccctcacaa 12060 agcccgtgag tgccttcctt tgatcgtttt cttgcgtaac cgtctcaaat atgctttgaa 12120 cggacgtgag gttaaggcca tccttatgca acgcctcatc aaggttgacg gaaaggttcg 12180 tactgactct acctttccta ccggctttat ggatgtaatc tccgttgaca agactggcga 12240 gcacttccgc cttgtctacg acatcaaggg ccgcttcact gttcaccgta ttactgctga 12300 agaagctaag tataagctct gcaaggtcaa gcgtgttcaa ttgggagcta agggggttcc 12360 cttccttgtc acccatgatg gacgtactat tcgttatcct gatcctttga tcaaggtcaa 12420 cgacaccata aaacttaatt tggaaaccaa caagatcgag tcgttcatta aatttgatac 12480 ttccgctcaa gttatggtta ccggtggtcg taacatggga cgtgttggta ccatcgttca 12540 tcgtgagcac catcttggtt cttttgaaat catccatgtt aaggatgcct tggaccgtga 12600 atttgctact cgtctttcaa acgttttcgt tattggtgag actggtaaga gctggatttc 12660 tttgcccaag ggtaagggtg tcaagctcag cattaccgaa gagcgtgacc gcagacgtgc 12720 ccttaaaggt ttggcttagt tctgaaataa gcgcaacata attattgtta tgcgaatata 12780 tttggtgttt cgttaatagc aaaacttagg actgtactaa taaacgtctt ttatatttta 12840 cacatgaagt aattagtaca gcatttttac attgtaaatt gtttttctgc tatagaaggg 12900 aactggttta agtcgtataa taaaaagttt cacatgtaga aatagaaatt gagattgaac 12960 aggtaattga ttacttagct atctattgca tcccaaaaac atagtatcat actctatcgt 13020 tgaatgcaca ttaattccta cttgcttaca tgaaattgga taattcattc ctgcatttaa 13080 atatttttct tttttctacc ttttttttat gcctattcac tatagtttgg tgttaagtat 13140 gcacaacgtg ctctaatgaa ctaaaaacag tcacaaagtt tcaccacaaa ccatatggaa 13200 tttccgtgcg tactttgcct ttaaccagga acagcaatgc ctcattctta cggtatcaga 13260 gctcgcaccc gttacacctt tcaacgtggt ttcagagagc atggtcaaat tcgtttgtcc 13320 acttatttga agacctataa ggttggtgac attgtcgaca ttaaggttaa tggtgctgtc 13380 caaaagggta tgcctcacaa gtactaccat ggcaagactg gtgttgttta caacgttact 13440 caatcttctg ttggtgtctt gatctacaag gttgttggta accgctacat ggagaagcgt 13500 gtcaatgttc gtattgagca cgttaagcac tctaagtgcc gtcaagactt cttggaccgt 13560 gttaaggcca atgaggctaa gcgtaaggag gccaaggctc agggtaagac tgttcaactt 13620 cgtcgtcaac ctgctcctcc tgctacggct catttcgttt ctactgagaa taacgagccc 13680 gtcactctcc accccgtcgc ctatgatact actatctagt tttaaaaaag aatgctgttc 13740 ctttggagtt tatcaaataa tcagacatta ttcgtagttt taattgttgt atttttgtaa 13800 atgcatttgt acatcgactt aacaaagtag tatatttatt gtgctcgtat cctcgtttag 13860 tataaaatat atatatgtac atcttagtta atcatgagta tccgttgctt aattatattt 13920 attagctgat ttgtcgttaa aagtatgaag gtataatcgt atttaaacga gtgttagttt 13980 agcaaattgt cggaatagaa tcgagaacaa tattgctcaa tgttgggctg gagtgcagga 14040 tcattaattt gtcgtaaata ttgaattttc aggcggacaa cagttttttg catctctacc 14100 atgtaacagg cccgataacc tatttaatca ttagagattg aactcgaaaa tcttgatcaa 14160 cttaccatca atagcactat caagcaactg aatgacaaac attcgtgaag gctgcaaaag 14220 gtcaagatgt ttaatctgca aagaagcaaa tgcatgaaat gctaatgcaa gtaatggttt 14280 gttatccgct gcaattatgt cagggattag tgaatctaat agatcgatag ctgtaaaaca 14340 atccagttcc ccaatcaagc acaaagtgca taatcctctc agaagataca atcttaaata 14400 gtttgatgag gccatttgaa ttatttccag caattcaaaa acagctcgat ttgacatttc 14460 agtcctctaa acactcacat tagcataagc ctaaaatcag ccttcacctt accatataaa 14520 tgtcaacctt taaaaacatc aactgcagca cccattcaat cgccagtttt tccggttctt 14580 ccaataattt atcaattcct tcaatggcct agtaacacgt ttagtatatg taggtacgaa 14640 tgttttgtta attacctgac ttaaatttcc caaactaata tccaacctta aagcgtagag 14700 ttgagttcga aaagtagaca tcttgtcgtg tctaaagcca tttaaatttt tcaaatacag 14760 ggtagcatcc atatgatttt tccttttcaa aattagcaaa ttttctgatt tttgtaaaca 14820 atgatttctt actcgtcaat cgccttagat aagtagataa atgttttttc tctgccatat 14880 aaatgtgcgc attgaaaaga acgctgtgat tgaacctcag cagtcgacaa aattgtagca 14940 acatctaacc atcttcctaa ttcagcgtac ctccatgcct tagttataag actttgtaat 15000 aggtcgagtg caggagaatc atggacgtga tttttttgat agatc 15045 //