ID SPAC955 standard; DNA; FUN; 5958 BP. XX AC AL353860; XX SV AL353860.2 XX DT 25-APR-2000 (Rel. 63, Created) DT 14-FEB-2003 (Rel. 74, Last updated, Version 3) XX DE S.pombe chromosome I cosmid c955. XX KW excision-repair protein; P-type calcium ATPase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-5958 RA Barrell B.G., Rajandream M.A., Wood V., Baker S., Churcher C.; RT ; RL Submitted (25-APR-2000) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9P6S7; Q9P6S7. DR GOA; Q9P6S8; Q9P6S8. DR SPTREMBL; Q9P6S7; Q9P6S7. DR SPTREMBL; Q9P6S8; Q9P6S8. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c955 is overlapped at its 5' end by csomid SPAC821 and CC at its 3' end by cosmid SPAC139. XX FH Key Location/Qualifiers FH FT source 1..5958 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c955" FT /map="I" FT CDS complement(<1..2826) FT /db_xref="GOA:Q9P6S8" FT /db_xref="SPTREMBL:Q9P6S8" FT /gene="SPAC955.01c" FT /gene="SPAC821.13c" FT /product="P-type ATPase (P4 type) (pers. comm. A. L. FT Okorokova Facanha); putative aminophospholipid translocase FT (pers. comm. A. L. Okorokova Facanha); 7 predicted FT transmembrane helices; similar to S. cerevisiae YMR162C" FT /EC_number="3.6.3.1" FT /protein_id="CAB88875.1" FT /translation="MKSSGIAGDSNGFETNFLNETTNREEDGAFNWNAADDGTNERRED FT IHVRFQDSALPLGIDENELDEIDINGDSKKLDSVEVDESHDVNSPSDSRLKSSFKSVLE FT LTANSMVSVLTPSTKTEQTSKGKGKKKKAHVSFLEGPVEEIPLDDIEPTSPREASPVFN FT GRPPIPPEFLKSRHKEFTIPNPLQFISNFLSNLFSRDTRYLKSSHGRIIIINPYDDSSQ FT IDERTGKPYMQNSIVSSRYNKYNFVPLQIIAQFSKTANCYFLLIAIMQMIPGWSTTGTY FT TTIIPLLIFISIAILREGFDNYRRYRQDRVENRIQTQVLRHVDVDPPIVEEHSSFFRRR FT RWRRSRSQESASRSTIRSTDEREPERTSEDPPQLPPSPSSPSSPALSVKPNIDPQPPLY FT NSTLTTTRSIPANKPTFFWASCDRKDVRVGDIIRLTSDQTLPADVIALSSPNPNGAIYI FT ETAALDGETSLKTRLVNSTLRSLCKDINDLIRLSGTCTVEDPNGDLYNFNGSMKLDSIQ FT GEIPLSNNDVLYRGSNLRNTSELFALVIFTGEESKIRMNAVRNVSVKAPSMQKVTNRIV FT IFIFALVVSMAIYCTAAYFVWQKKVERKLWYLTNSKLSFVPILVSFIILYNTMVPISLY FT VSMEIIRVFQTFLVQSDIDLYYPENDTRCEVRSSSILEELGQVTHVFSDKTGTLTDNIM FT LFRNLSVGGFAWQHVGAENPKLVSTSQKSDDLDGEAKPPQLENIQGTTIQLLQYVHDNP FT HTTFSKRVRIFLLNLAICHTCLPSFDEENQIYKYQSISPDELALVHAAQQLGYIVIDRD FT IDSLTIRLHYPLDPHSHPIAKTYRILNIIEFTSKRKCMSVIVRMPNGRICLFCKGADSA FT IIKRLRLSNLAKRKDKSVTKAEQARKSIEIDKAIIRNSQSTSRPSLTASRPSLSRRRND FT YINNVTSWLDER" FT misc_feature 1..214 FT /note="nominal overlap with cosmid SPAC821, EM:AL121770 S. FT pombe chromosome 1" FT misc_feature complement(757..777) FT /note="PS00154 E1-E2 ATPases phosphorylation site" FT 3'UTR complement(3601..3863) FT /note="mRNA from AU007395" FT CDS join(complement(4228..>5958),complement(3864..4167)) FT /codon_start=2 FT /db_xref="GOA:Q9P6S7" FT /db_xref="SPTREMBL:Q9P6S7" FT /gene="SPAC955.02c" FT /gene="SPAC139.01c" FT /protein_id="CAB88876.2" FT /translation="YRMDASGPANYGESKPTLLYTNQRDHLDRLCDQVKFYLDQCNVEY FT FVAPYLAMAQLAYFLNGTSSPYIDAIYGSTDLLLFGVKKFITSMNTSSNVKISSDPSSP FT STQTTINSAAKSSFTWLDGNALLQDTNGLSWQQFIDSCLLCGTAISPTFPQIEGTFLIK FT SAMELVRMFGSAYRAVLHFAEIFPQPIFQDYLQQYKRAVCFSKFGIVMDTKGLTLPPVP FT TESVPNDINIYFGTRLPNEIYFYISRGLIPCKMIGALVSGCFSDPVSILEQHIGDKASQ FT GTFSGANSGLNPGRDNAAAVAAVDQRRFVDDLEEIWSQGLNLLTQPLNRFYQARDIVSL FT HGHNQQASLKVMHSYDPPLYNDTRAWMIYEENLPSYLSSNFLKEEPVVLFDLLRALNDP FT VFVKKSFCTEGNIGIKNPPKHPLSSTAEIVLSSCYRFLQIRSFVLTSHQLTSWGCPLLK FT ALENCHLQNQTSVVVLFELLRLRQLKEPSLLSSSSASIADLSITSATEFLAKVATFLPI FT KQRPEVSKISIVDENLLQFYQLQTSFGSNLKELMAMILASVILNRNVDKSKIDPKLIRK FT TLPFQNINGSLVSGFVVKRFFEIISKEQEASSQQENIQKAYEIIEKEFPTIGSAENHIA FT QFLEFWKTFMEGVKEAENTSAIGKLVLSKLFLTNQWIFSLGL" FT misc_feature complement(4168..4180) FT /note="ctaaccaatttag, splice branch and acceptor" FT intron complement(4168..4227) FT /note="confirmed by mRNA" FT misc_feature complement(4222..4227) FT /note="gtacgt, splice donor sequence" FT misc_feature 5729..5958 FT /note="nominal overlap with cosmid SPAC139, EM:AL132667 S. FT pombe chromosome 1" XX SQ Sequence 5958 BP; 1902 A; 1071 C; 1183 G; 1802 T; 0 other; cctttcatcg agccaagaag tgacgttgtt aatataatca tttctgcgcc gagacaaact 60 aggtcttgaa gccgttaaac ttggcctcga agtgctctgg ctattccgaa taatcgcctt 120 gtctatttca atgcttttac gagcctgttc agctttggtt actgatttgt ccttccgttt 180 ggcgaggttg gaaagacgaa gtcgctttat gatcgctgaa tctgctcctt tacaaaacaa 240 acatatcctt ccgttaggca tgcgtacgat aacagacata catttccgtt tgctcgtaaa 300 ttcgataatg ttcagtattc ggtatgtttt ggcaatcggg tgagagtgtg ggtccaatgg 360 ataatgcaat cggattgtca agctgtcaat gtctctatca ataacaatgt aacctagctg 420 ttgagctgca tgcacaagag ccaactcatc tggtgaaatg gattggtatt tataaatttg 480 attttcttcg tcaaaagaag gaaggcaagt atgacaaatc gccaaattta aaagaaaaat 540 tcgtacccgt tttgaaaaag ttgtatgagg attgtcatgt acatattgaa gcaattgaat 600 agtggtaccc tgaatgttct caagctgcgg aggtttggct tcaccatcaa ggtcatcact 660 cttttgtgat gtggatacaa gcttaggatt ttccgcgccg acatgctgcc atgcaaatcc 720 gccaacagat agattacgaa aaagcataat attatcggtt aaggtaccag ttttatcgga 780 gaaaacatgt gtaacctgac ctaactcttc aaggattgag gaacttcgaa cttcacatcg 840 agtatcgttt tctggatagt acaaatcaat gtcactttgc actaaaaatg tttgaaaaac 900 acgtataatc tccatagaaa cgtaaaggga aattggaacc attgtattgt aaagaatgat 960 aaaagaaaca aggataggaa caaatgaaag tttactgttg gttagatacc acagcttcct 1020 ttccactttt ttttgccaaa caaaataggc agcagtacaa tatatagcca tgctaacaac 1080 caatgcaaaa atgaaaatta cgattctatt agtgaccttt tgcattgatg gtgcctttac 1140 actaacgttt cgaacagcat tcatgcgaat tttggattcc tcgccagtaa aaataaccaa 1200 agcgaacaat tctgaagtat ttcgcaaatt cgagcctctg taaagaacat cgttattaga 1260 caatggtatt tctccttgta ttgagtcaag tttcatactt ccattaaaat tgtaaagatc 1320 accattcggg tcttcaacag tacacgtacc actcaatcgt attaaatcat taatgtcttt 1380 gcaaagagag cggagcgtgg agtttactag tctagttttc aaggatgttt caccatcaag 1440 tgcagctgtt tcgatgtaaa tagcgccatt aggattggga ctcgacaaag ctattacgtc 1500 tgctggaaga gtttgatcac ttgtcagtcg aataatatca ccaactcgaa catctttccg 1560 atcacagcta gcccaaaaaa aagtaggctt atttgcaggt atggagcgag tagtagttaa 1620 agtagagtta tacaaggggg gttgcgggtc aatgttaggt ttgacactta acgccggaga 1680 agagggggaa gagggagagg gaggaagttg aggtggatct tctgaggttc tttcaggttc 1740 tctttcatca gttgatcgaa tagtactcct tgaagcagat tcttgagatc tacttcttct 1800 ccaccgccga cgacgaaaaa aagatgagtg ctcttcaaca ataggcggat ctacatcaac 1860 gtgacgaaga acttgagttt gaattctatt ttctacgcgg tcttggcgat atcgccgata 1920 attatcaaac ccttcacgaa ggattgcaat tgaaataaat ataagtagag ggatgattgt 1980 agtgtaagta ccggtggttg accaacctgg aatcatttgc ataatagcaa tcagcaaaaa 2040 ataacagttg gcagtttttg aaaattgagc aatgatctgt aaagggacaa aattgtattt 2100 gttgtaccga gacgaaacaa tgctgttttg catatagggc tttcctgtac gctcatcaat 2160 ttgggaggag tcatcgtaag ggtttatgat aattattcga ccatgactcg acttcaaata 2220 ccgtgtatca cgcgaaaaca agttggataa gaaattggaa atgaactgca aaggatttgg 2280 tattgtaaac tctttatgcc gcgactttaa aaattccgga ggaattggag gacgcccatt 2340 gaacacaggt gatgcctcac gaggagaagt aggctcaata tcatctaatg ggatttcttc 2400 cacaggtccc tctaggaagg aaacatgtgc tttcttcttt tttccctttc ctttcgaggt 2460 ctgctcagtt ttggtagatg gagttaacac tgaaaccata gaattagctg tcagttctaa 2520 taccgacttg aaagatgatt tcagtctgct atctgatggc gaattaacat cgtgtgattc 2580 gtcaacttca acagaatcca attttttgct gtccccattt atatcaattt catcaagctc 2640 attttcatca attcctaatg gaagagcgct atcctgaaat cgtacatgaa tatcctctct 2700 tcgttcgtta gtgccatcat ctgcagcgtt ccaattaaat gcaccatctt cttctcgatt 2760 agtcgtttca tttagaaaat ttgtttcaaa tccattggaa tcgcctgcga taccactaga 2820 cttcataagt gcatctgaac aaataatgtt ggttgttgct agattcacac caaatggaaa 2880 atcaaactat tatttgatgg tagcttgctg aaccacacgg gacattgtgc tacgccttac 2940 aaaatgagac tacactaata gcagttcatt aaagttaagc ttaatgaact ccacagtaat 3000 ggcttacgct tgcttaagac tttctaaata gtgtgtcaag tctcgcaata tgcaatgtaa 3060 acaataagtt acagagattt tgattaactg tcagttactt caactactac cgtttatatt 3120 taaaaactga cattgtcaga attttgttaa ctatcgacaa atattattag atgttagtgt 3180 gagaattgga gattctcaaa gctagcaagt tgtcgtaaac agcatctata cagtaaatat 3240 cttaaaacaa aattatacta tccagcttgt aagtggttaa actaagcaag tctttctgta 3300 agcacaaggt ttttaattcg tgaaattgat taggtaaaat tatgtttctg atgcgaattc 3360 ggttttttgt gagtgagcca gtacacataa actaaaatct aaaaaatccg cttgaactta 3420 tcaaataagt taaaaattat tcccatacta tagtgtaaaa acaacggtat aagttatttt 3480 ttgacaggta agtgtattgc taaaaataaa tatagtatcc tttagaacaa attaaactat 3540 atctataaaa aagtttaaac atcattgggt tttcattcaa agctgtgctt ctctattttt 3600 ctttcccttc cttttttcgg gaagaaacaa tgtagttgaa tattaatatt aacaatactt 3660 tacttgttcg aaataatatc tcaacaataa tcaagtgtaa tcacatgagt gtatagcatg 3720 ttgaaaatgc ttcgacagta tgagggaaag taattcatgt aaatgtgcat tatgactgtg 3780 ccgaatgtgt taaaagctcc cgctaaaaga aggatacttc tctcactaat ttatttctat 3840 tcataaacta ctagttctgg tcttcataag ccaagagaaa atatccattg gttagtaagg 3900 aagagtttac ttaaaacgag cttgccaatg gcacttgtat tttctgcttc cttaacccct 3960 tccataaaag ttttccagaa ttccaaaaac tgggcaatat ggttttccgc agatccaatg 4020 gttgggaatt ccttttctat tatttcataa gccttctgga tgttttcctg ttgagaactg 4080 gcttcttgct cttttgaaat gatttcaaaa aagcgcttga caacaaaacc tgagacaagg 4140 ctaccattta tgttttgaaa aggtaagcta aattggttag aaactttaaa tataaacaaa 4200 aaaggagaaa gagaaaaaaa cacgtacgtt tttcggataa gtttaggatc tattttgctt 4260 ttgtcgacgt ttctgttcaa gatgaccgag gcaagaatca tggccatgag ttccttcaag 4320 ttggatccga agctagtttg tagctgataa aactgtaata aattctcatc aactatagaa 4380 attttactaa cttcaggtct ctgttttatt ggtagaaatg ttgcaacctt tgccaagaac 4440 tctgtggcag aagttatgga taaatcggcg atgcttgcac ttgaggagga aagtaaagag 4500 ggctctttta attgtcttag tctaagaagc tcgaataaaa caacaacgga agtttggttc 4560 tgtaagtgac aattttccaa cgcttttaac aaggggcatc cccaagatgt taattgatga 4620 gatgttaaaa cgaaagatct aatttgcaaa aatctgtagc aagaacttaa gacgatttcc 4680 gcagtagaag acagaggatg cttgggaggg ttttttatgc caatattacc ttcagtgcaa 4740 aagcttttct ttacaaatac cggatcgttc aaagcacgta gtagatcaaa taatacaacg 4800 ggttcttcct ttaaaaaatt actagataaa taactcggta agttttcttc gtatatcatc 4860 catgctctcg tatcattata caaaggagga tcatagctgt gcattacctt caaagaagct 4920 tgctgattat ggccatgaag ggaaacaata tctctggctt ggtaaaatct gttaagcggt 4980 tgggtcaata aatttagccc ctgcgaccat atttcttcta aatcgtcaac aaatcgccgt 5040 tgatccacag ccgctacagc tgctgcattg tcgcgaccag gatttaaacc agaattagca 5100 ccagaaaatg ttccttgtga tgctttgtca ccaatatgtt gttcaagtat ggaaacagga 5160 tcactaaaac atccagatac taaagcacct atcattttgc aaggaataag accgcgagaa 5220 atgtaaaaat aaatctcgtt gggaagacgg gtgccaaaat atatatttat gtcgttagga 5280 acagattctg tgggaactgg tggtaaagtg agaccttttg tatccatcac aattccaaac 5340 ttagagaaac aaacagccct tttgtactgt tgaagataat cttgaaaaat gggctgggga 5400 aagatttcag caaaatgaag gactgcacga tacgcagaac caaacatacg aacaagctcc 5460 attgcgcttt taattagaaa agtaccctca atctgaggaa atgtaggaga aatagcagta 5520 ccacacaata aacaggaatc gatgaactgc tgccatgaaa ggccgttcgt gtcttgaaga 5580 agggcatttc catctaacca tgtgaatgaa cttttagcgg ccgaattgat agttgtttga 5640 gttgatgggg atgaagggtc agatgagatt ttaacgttag aagatgtgtt catagaagta 5700 ataaattttt taacaccaaa aagaagcaga tccgtggaac catagatggc atcgatgtaa 5760 ggtgaagatg tgccgtttag aaaatatgct aattgagcca tagccagata cggggcgaca 5820 aaatactcca cattacattg atctaagtaa aactttacct ggtcgcaaag acgatctaaa 5880 tgatctcgct gattggtgta caaaagtgtt ggtttacttt cgccgtaatt cgcaggacca 5940 cttgcgtcca tgcggtac 5958 //