ID SPAC8E11 standard; DNA; FUN; 15350 BP. XX AC AL021817; XX SV AL021817.2 XX DT 12-FEB-1998 (Rel. 54, Created) DT 29-NOV-1999 (Rel. 61, Last updated, Version 5) XX DE S.pombe chromosome I cosmid c8E11. XX KW dehydrogenase; dmp1; dna damage checkpoint protein; glycosyl hydrolase; KW lysophoshpholipase; meiotic recombination; pdmc1; KW putative chaperonin cofactor a; rad24. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-15350 RA McLean J., Harris D., Wood V., Barrell B.G., Rajandream M.A.; RT ; RL Submitted (11-FEB-1998) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Hinxton Hall, Hinxton, Cambridge CB10 1RQ E-mail: barrell@sanger.ac.uk XX DR GOA; O42634; O42634. DR GOA; O42878; O42878. DR GOA; O42881; O42881. DR GOA; P42656; P42656. DR GOA; Q9Y6Z9; Q9Y6Z9. DR GOA; Q9Y703; Q9Y703. DR SPTREMBL; O42878; O42878. DR SPTREMBL; O42881; O42881. DR SPTREMBL; O42882; O42882. DR SPTREMBL; O42883; O42883. DR SPTREMBL; Q9Y6Z9; Q9Y6Z9. DR SWISS-PROT; O42634; DMC1_SCHPO. DR SWISS-PROT; P42656; RA24_SCHPO. DR SWISS-PROT; Q10170; YAUH_SCHPO. DR SWISS-PROT; Q9Y703; TBCA_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c8E11 is overlapped at the 3' end by cosmid SPAC26A3. XX FH Key Location/Qualifiers FH FT source 1..15350 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c8E11" FT /map="IL" FT misc_feature 1..199 FT /note="nominal overlap with cosmid SPAC26A3, EM:Z69240 FT S.pombe chromosome 1" FT CDS complement(4..426) FT /db_xref="SWISS-PROT:Q10170" FT /label=SPAC8E11.11 FT /note="SPAC8E11.11, len:140, SIMILARITY:Arabidopsis FT thaliana, O49532, predicted protein., (326 aa), fasta FT scores: opt: 166, E():9.8e-05, (27.8% identity in 126 aa)" FT /partial FT /gene="SPAC8E11.11" FT /product="conserved hypothetical protein" FT /gene="SPAC26A3.17c" FT /protein_id="CAB40198.1" FT /translation="MDAPLVNESVEFQESQESLSLLEASKQLDLKKIQNLVDQGAVTSA FT IDIESGKNALHFIASYAEKETEVQAIEVTKWILSNGGVWNGIDRANETPGCIARRRNLL FT RLYETIVDAGVRSELLLSLLERKELTNEQLDTNEKYL" FT CDS complement(1161..1928) FT /db_xref="GOA:Q9Y6Z9" FT /db_xref="SPTREMBL:Q9Y6Z9" FT /label=SPAC8E11.10 FT /note="SPAC8E11.10, len:254, SIMILARITY:Candida albicans, FT SOU1_CANAL, sorbitol utilization protein sou1., (281 aa), FT fasta scores: opt: 809, E():0, (52.0% identity in 256 aa)" FT /gene="SPAC8E11.10" FT /product="sorbitol utilization protein sou1." FT /protein_id="CAB40197.1" FT /translation="MTSMFSLKGKTTLITGGSGGIGFSIAKAFAAAGSNVGLLYGRNKK FT ALEYAAELRDKHGVQAKAYSCPIENRSAVIETTNQAVEELGGRLDVMIANAGIAIPHLS FT LEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLIFTASMSGHIANWPQQWASY FT HATKAAVKHLARALAVEWAPFARVNSVSPGYIDTDLTLYADENLRKKWKEYTPQARIGL FT PDELPGAYLYLASDASSYCTGSDIIVDGGYCSR" FT misc_feature complement(1185..1274) FT /note="Match to PF00678 adh_short_C2, Short chain FT dehydrogenase/reductase C-terminus Score 40.27" FT misc_feature complement(1335..1901) FT /note="Match to PF00106 adh_short, short chain FT dehydrogenase Score 186.32" FT misc_feature join(2640..2896,3150..3348) FT /note="was SPAC8E11.09c, len:151; questionable ORF, but now FT thought to be not coding" FT misc_feature 2897..2902 FT /note="gtatta, splice donor sequence" FT misc_feature 3117..3149 FT /note="ttaactattaaatatacttattcaaaaaaaaag, splice branch and FT acceptor" FT misc_feature 3460..3762 FT /note="was SPAC8E11.08c, len:100; questionable ORF, but now FT thought to be not coding" FT CDS join(5092..5160,5224..5310,5463..5666) FT /db_xref="GOA:Q9Y703" FT /db_xref="SWISS-PROT:Q9Y703" FT /label=SPAC8E11.07c FT /note="SPAC8E11.07c, len:119, SIMILARITY:Oryctolagus FT cuniculus, T1CA_RABIT, tcp1-chaperonin cofactor a., (107 FT aa), fasta scores: opt: 160, E():0.0009, (32.7% identity in FT 104 aa)" FT /gene="SPAC8E11.07c" FT /product="putative chaperonin cofactor a" FT /protein_id="CAB40194.1" FT /translation="MSNTVRSLVIKTNVVKRIIKDVELAHIDINEAEKRVQSKIDNGED FT SAEIEHQKFVLKKHLEALPDALVRLRNATNDLESISSDSAYEGTPELEQANEYLEKAKE FT VIEKEQTNFPTNGYH" FT misc_feature 5161..5166 FT /note="gtaagt, splice donor sequence" FT misc_feature 5203..5223 FT /note="ctgacctcaacaattgtttag, splice branch and acceptor" FT misc_feature 5311..5316 FT /note="gtatgt, splice donor sequence" FT misc_feature 5443..5462 FT /note="ctgacattctggacctctag, splice branch and acceptor" FT misc_feature complement(5812..5902) FT CDS join(complement(6013..6038),complement(5948..5965), FT complement(5845..5902)) FT /db_xref="SPTREMBL:O42883" FT /note="SPAC8E11.06, len:24aa, questionable ORF but FT predicted from EST data" FT /gene="SPAC8E11.06" FT /product="hypothetical protein" FT /protein_id="CAA17027.2" FT /translation="MLLVSRNICRIKGNTIDWRNLKKDLSMTWIILK" FT misc_feature complement(5903..5919) FT /note="ctaactggcaaaattag, splice branch and acceptor" FT intron complement(5903..5947) FT /note="confirmed intron" FT misc_feature complement(5942..5947) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(5948..6012) FT /note="exon" FT misc_feature complement(5966..5984) FT /note="ctaaaatttaaatctctag, splice branch and acceptor" FT misc_feature complement(6007..6012) FT /note="gttgtt, splice donor sequence" FT CDS 6216..7232 FT /db_xref="SPTREMBL:O42882" FT /label=SPAC8E11.05c FT /note="SPAC8E11.05c, len:337" FT /gene="SPAC8E11.05c" FT /product="hypothetical protein" FT /protein_id="CAA17026.1" FT /translation="MASPPILSRESPVLGLEEEKKSDGGNSEVNIDPSASSSLKEDVDG FT EEGADTKIDPHLLEEDDLNPVEDYDSKNQKVVEASKLDLDATSTVWAESLSINREASIS FT QSGIVDNEMEKKVEEEEEFDEFDDFGEFEHDIPICTFMGDWQDDFQDAVNSVFGLPETP FT KTIEESSLDPDRILSLWQKLIEMPVLQRPDWLRSSIRHIFLVTMGLPVDLDELLPTPST FT QGSFNSSRTIELSSVTLPKSEDEPYLDYSAARRLCSINKDALTHRSHESLQQHIELLES FT TLQQAVEVARYWTDKRDSALSDKLLYETVVDDLVQHSKRLRNSGRKFLSRRSTSSHK" FT CDS join(8700..8772,8844..8928,9040..9087,9143..9288, FT 9403..9725) FT /db_xref="GOA:O42881" FT /db_xref="SPTREMBL:O42881" FT /label=SPAC8E11.04c FT /note="SPAC8E11.04c, len:223, SIMILARITY:Caenorhabditis FT elegans., Q21224, k04g2.5 protein., (333 aa), fasta scores: FT opt: 552, E():5.7e-30, (41.2% identity in 211 aa)" FT /gene="SPAC8E11.04c" FT /product="lysophospholipase" FT /protein_id="CAA17025.1" FT /translation="MTAKLNSVIINPSVAHTATVIFLHGLGDSGQGWSFMANTWSNFKH FT IKWIFPNAPSIPVTVNNGMKMPAWYDIYSFADMKREDENGILRSAGQLHELIDAELALG FT IPSDRILIGGFSQGCMVSLYAGLTYPKRLAGIMGHSGFLPLASKFPSALSRVAKEIPIL FT LTYMTEDPIVPSVLSSASAKYLINNLQLKCLDRPFEGDAHSLSSESFMAMYKFTQTVIG FT SP" FT misc_feature 8773..8778 FT /note="gtatga, splice branch and acceptor" FT misc_feature 8826..8843 FT /note="gtaacacatttatcttag, splice branch and acceptor" FT misc_feature 8929..8934 FT /note="gtaagt, splice donor sequence" FT misc_feature 9021..9039 FT /note="ctaattattttgtatatagtc, splice branch and acceptor" FT misc_feature 9088..9093 FT /note="gtaagt, splice donor sequence" FT misc_feature 9128..9142 FT /note="ctaaaacttttttag, splice branch and acceptor" FT misc_feature 9289..9294 FT /note="gtatga, splice donor sequence" FT misc_feature 9382..9402 FT /note="ctaactcgtcattttctctag, splice branch and acceptor" FT CDS join(10276..10348,10423..11348) FT /db_xref="GOA:O42634" FT /db_xref="SWISS-PROT:O42634" FT /label=spdmc1 or dmp1 FT /note="SPAC8E11.03c, len:331" FT /gene="spdmc1" FT /product="meiotic recombination protein" FT /gene="dmp1" FT /gene="SPAC8E11.03c" FT /protein_id="CAA17024.1" FT /translation="MEEFAEGNDDEQMIFSDIEDLTAHGIGMTDIIKLKQAGVCTVQGV FT HMSTKRFLLKIKGFSEAKVDKLKEAASKMCPANFSTAMEISQNRKKVWSISTGSEALNG FT ILGGGIQSMSITEVFGEFRCGKTQMSHTLCVTAQLPRDMGGAEGKVAFIDTEGTFRPDR FT IKAIAERFGVDADQAMENIIVSRAYNSEQQMEYITKLGTIFAEDGQYRLLIVDSIMALF FT RVDYSGRGELSERQQKLNIMLARLNHISEEFNVAVFVTNQVQADPGAAMMFASNDRKPV FT GGHVMAHASATRLLLRKGRGEERVAKLNDSPDMPEAECSYVITPGGIADVS" FT misc_feature 10349..10354 FT /note="gtaagc, splice donor sequence" FT misc_feature 10408..10422 FT /note="ctaacataatcaaag, splice branch and acceptor" FT misc_feature 10707..10730 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS join(12212..12281,12689..13431) FT /db_xref="GOA:P42656" FT /db_xref="SWISS-PROT:P42656" FT /label=rad24 FT /note="SPAC8E11.02c, len:269" FT /gene="rad24" FT /product="dna damage checkpoint protein Rad24p" FT /gene="SPAC8E11.02c" FT /protein_id="CAA17023.1" FT /translation="MSTTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERN FT LLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILT FT VLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATA FT ELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLI FT MQLLRDNLTLWTSDAEYSAAAAGGNTEGAQENAPSNAPEGEAEPKADA" FT misc_feature join(12227..12281,12689..13341) FT /note="Match to PF00244 14-3-3, 14-3-3 proteins Score FT 513.96" FT misc_feature 12282..12287 FT /note="gtatgt splice donor sequence" FT intron 12282..12688 FT /note="confirmed intron" FT misc_feature 12676..12688 FT /note="ctaatatatccag, splice branch and acceptor" FT misc_feature 12748..12780 FT /note="PS00796 14-3-3 proteins signature 1" FT misc_feature 13264..13323 FT /note="PS00797 14-3-3 proteins signature 2" FT CDS 13991..15350 FT /db_xref="GOA:O42878" FT /db_xref="SPTREMBL:O42878" FT /label=SPAC8E11.01c FT /note="SPAC8E11.01c, len:>452, FT SIMILARITY:Schizosaccharomyces pombe, O59852, invertase, FT (581 aa), fasta scores: opt: 1134, E():0, (46.5% identity FT in 376 aa)" FT /partial FT /gene="SPAC8E11.01c" FT /product="glycosyl hydrolase; invertase" FT /protein_id="CAA17022.1" FT /translation="MPVRPCIHFTPPEGFMNDPNGLVYSNGKWHLFFQWNPTGNQAGNQ FT HWGHAVSKNLYKWKLLPTALAPGDDHGLMFSGSAVIDKTNSSGFFESGFFSRKSVDPEE FT RIVLIYTTHYDNRETQNIAYSLDGGITFIKYKKNPILDIKESQFRDPKVFWHEESRAWI FT MVVVLAQKYKVLFYHSLNLRDWVKLSEFGSAGVLGYQYECPDFVRLPIEGTDEFRWVLI FT VSINPGSSINGGSMVQYFIGDFDGQTFTPIDSASRILDCGHDCYATQTFGNAPDGRVIS FT ISWASNWNYTNDVPMRMKHRGMFTIPRELTLCYTHLNQETRGLVLRQRPVNLHHLYYPD FT SLPAPLLLNRVCEFPTVWTSATVFYLAVSIPKSIVLESPMEFLCLTWSTTPDVETSKEY FT FELGYRFHDGAVYVERGVCSSSWKYPLYPERCTSSVPPSSYEDNYILEIEAVVD" FT misc_feature 14012..14053 FT /note="PS00609 Glycosyl hydrolases family 32 active site" FT misc_feature 14012..14983 FT /note="Match to PF00251 Glyco_hydro_32, Glycosyl hydrolases FT family 32 Score 376.91" XX SQ Sequence 15350 BP; 4815 A; 2752 C; 2913 G; 4870 T; 0 other; ttgcaagtat ttttcgttag tatccaattg ttcgtttgta agttccttcc tctctaagag 60 agaaagtaat agctctgatc gaactccagc atccacgatt gtttcgtaca agcgcaataa 120 attcctacga cgtgcaatgc atccaggagt ttcgttagct ctatcaatac cattccagac 180 tccaccatta gaaaggatcc atttcgttac ttcaatagct tgaacctctg tctccttctc 240 agcgtatgag gctataaaat gtaaagcatt tttgccagac tcgatatcga tagctgaagt 300 aactgccccc tgatccacta gattttgaat tttttttaaa tctagttgtt tggaggcttc 360 caagagactt aaggactctt gagattcttg gaattccacc gattcattca ctagtggagc 420 gtccatttta atatgaagat ggcaggtatt ttttactcgt agctttaagc tgaatgcttt 480 gctggactta gaaattaagg gatttttttg ttaaacaata agacatttta gataccgttg 540 taatcattta cataacgaag atgaaataaa aataaaaaat tcttctttgt tctatcaagt 600 gtttcaaaga catagaatgt actcatgagc tataggataa agaaaactag caaggaaatg 660 gcattaaata attaaaatta ttctacctcg atatattgta caaataaccc atctgaaaca 720 tgcaaaaaga atagcatacc gttgatttat tttaacttcg gcgatgtagt attactaagg 780 tgtgcttatg caagttagag aaagaaagag ttagtttatc atacacagtg tttatgttaa 840 ctatagtgat aaaacttttt agatttgtta gatgataatt aaaaagcatt agaaacttca 900 aaacgacaaa tcgggagagg aagcatatag aatagcagtt tgttttaaaa aaaccaattc 960 aaattacatg ctgccatgag tgtagagatc taagctccaa caacaaaatg agtacggtag 1020 aggggaaatg ttttaaaaat gaaacagatc cagaagaaga acctttttga cgtagctgtt 1080 ttggattata ggctcaaggg aggttcaaaa attcaagttt ttaattatga ttatagaggg 1140 aaatattaat agtaaagaaa ttaacgagaa cagtaaccac cgtcaacaat aatgtcacta 1200 cctgtgcagt aggaactagc atcggaagca aggtataagt aagcaccggg aagctcatca 1260 ggtagaccaa tacgggcttg aggagtatac tccttccatt tcttacgcaa gttttcatca 1320 gcgtacagag tcaaatctgt gtcaatgtag ccaggagaca cggagttgac acgagcgaaa 1380 ggggcccact caacggcaag agcgcgagcc agatgtttga cagcagcctt ggtagcatga 1440 taagaagccc attgttgagg ccaattggca atgtgaccac tcatactagc cgtgaagatc 1500 aaactgccct ttccttgctt cttaaaatga tggccagcag cctgtgcagt ataataagca 1560 ccgttcaggt ttatgcccac gaccttggtc cagatgtctt cattcttatc ttccaaggat 1620 aagtggggaa tcgcaatacc agcattagca atcattacat ccaaacgacc acccaattcc 1680 tcaactgcct ggttggtggt ttcaattaca gcactacggt tctcaatggg gcaagaataa 1740 gccttggctt gaacaccatg tttgtcgcgt aattcagcag catactcaag agcctttttg 1800 ttacgaccgt acaaaagacc aacattagat ccagcggcag cgaatgcttt agcaatcgaa 1860 aatccgatac cgcctgaacc accggtgatg agagtggttt tacctttaag agaaaacata 1920 gaagtcatta tgagatatat gaaaaaaacc gaacgatgta ataattatta agaaaaaaaa 1980 aaccccacaa aaatatttta gcgaacaaaa agtggtagta aaatttatga acaccggttt 2040 aatagctttt gattagatga aaagcaaaaa tttttttccg aaagattttt aatagtaaga 2100 gaaggcactc ttggcaacca gaaagaaaaa ttgctttgtg gcaatggacc caaggctttt 2160 atacaaaaaa tttcctcttt ttgacacaca gaaagagcag tcctaaatag tgttgttgga 2220 aaaaatatat gtgtaaataa acgatttgca aaggaggaaa aaaatagcgc aaatagacaa 2280 aacggtcgtt ttactcgagt gacactagta gatccatcaa accgataata tcgggtattg 2340 tgatggctga gtggaaagtt tgaatgaata actgcgagaa actaggctac acacatagga 2400 gtcaaaatag tgtaacccgc aaaatataga aatccaattc ataaatgtga gaatgacaat 2460 tcatttatta taaattggaa actcatttca tcttttcttt ttttttcgtg aaatactacg 2520 agtttctctt aaatccgggg agaaagtacg gataaaatcc catgattaag aaggggagcg 2580 tcggacgaca aaggtagctt aaattaacaa atggccaagt gattttggcc atttggggta 2640 tgatttcgct tctctcgatc aagatgcaag cgattaaaga actggactcg gaagaggaac 2700 caaaagttgg cgagttctat aagggcaaca caatttcgtt ttgcaaaaat tcgcttgaaa 2760 atgatactca attacctcaa attctcttct gcattaatcc taaatccatc cgaaattttc 2820 tttttccacc catagcaata tcagatccga aagaagctca aaatcactgt tcgtcttact 2880 attatattaa gtaccggtat tatattgtac agtcaagatg cgggatgatg ggaagaatgt 2940 ctttttaaat attatctaaa aaaggtaata gttacatgtt aataactata tatatatatt 3000 tccagactat actagtaaag taacggttga aaaaatgtgt aagtagaaga actattaaac 3060 ttaattgcgt tcagtagttt ctgcatggtt tttaactaaa accatgttcg atattgttaa 3120 ctattaaata tacttattca aaaaaaaaga acaccagatt ctcctgtcac gcatctaggt 3180 ttccagtttt tcatctgttc cctcattaac gttctaccag ttgcccgcat tgttccagaa 3240 gatcaaccca aatctgaacg agatgcaata gcctggagac taccagagtc ttcattttac 3300 ccgaccgaga tcgtttccat ggtcggaatc gaacactgga atacttgaga cttggcgaaa 3360 aatgagtatt atacaaggga agaaaagaag aagaaaataa aaggataatt tatgttttac 3420 ggcaataaaa taatgaatta ataagcaaga agtattaaaa tggactttac cactaatgag 3480 gttctgttta gtaggttagt agtctgtaaa aatttgacaa gaaaaaattt tccgtttctt 3540 caattgaagg ggagctccat tttaagattt gaatggtctg aagaaatgta tagattgtat 3600 atatggttgt taccgtaccg aaaagactat aatgaaaagt ttaagggaat tctaataaat 3660 ccttctattt ttcttcttgg ctgtcatcat gttcaaatat gcagttttcc gaacaacctt 3720 gtatttttca tgggaaaatg cgatttgttt cgtttttgtt agctttgttt gaataccatt 3780 ttttaactta tgcgatactt tctaaacgga gttcactttt taaattaatc tatatcaact 3840 gagccgttca attcaatccc tttttttata tcagcttgta aacaaacgtt tcaggaaatg 3900 agaaagttca aaaagcgtta aacagtgaat gcttgtatgt tgagctttga cttgtttgca 3960 aatgtttaat tgtcgaaatt catcatctgt atctgtccaa agttgtgaac tgacaagata 4020 gagattaatt tgttagatca cgcaaagtac gatatgttta aaatgcttat accaggttgc 4080 agtctttgtt tttcatagct ttgcaaaaaa aaaagtggat taaaatacta atgactacta 4140 gggttcgaaa ggttgctgtt cgattatgaa aaagcgttca ctcttttatc tcccaagcga 4200 tgatttgaat ttttgatgca tctgtggcgt agaatatgat caatcatttt agaaaacaga 4260 ataatattca aggatccatt taaagtccta tatagagtaa aggattttag ctattatcct 4320 cacattgaag actggtccca ttataccgaa gtctgttgac attaatatcg ttgtaccggc 4380 tttttgagta gactaaatat cttgtacagg atctactttg aaagagggta ttaggtcctt 4440 tttttatttt ttgttttcca aggattaaga tagaagctga atttttttgg ctaggcaaag 4500 ttgactaatt attaacagac tgtggttcga gtgattagca atcaacctat ccttatatga 4560 aatcgtgctt ttaacaattt cggttgttgg gagataactg tgtgtacgga gaatcagaaa 4620 tttttgtttt ggaaaatatt tgtaaaagca attacgtcta aatataatga attacgcgct 4680 agaaaatttt catatagctg aatcccgaaa agctcaacct cccatatgat tgagcatcca 4740 taaaagcatg aaatttttgg tacaagtatc ggtgaatcgt aaatttagaa ataactgagc 4800 aattttatct catacataat ataggaatga aacgtttgtc gatcaataac tagtctgata 4860 cttgatgcaa acatttaatc aaataccctt ttttaggaat ttaaggaaag ggctttaaaa 4920 gaaattgaaa acttaactag aactagcttt tcttaatcgt ttctcgtaac ttatcaccac 4980 acgctaacat tagcattcct ttgtattcca acgttaccta cactgcagtc aaggaaaatc 5040 atccaaccta acattattcc tttgcatatc gcgtccattt tattttctac catgtcaaat 5100 actgttagga gcttagtcat caaaactaat gtcgttaaaa gaattattaa agatgtagaa 5160 gtaagtttcc tttttggatc gcgattttgt agatggaatt ttctgacctc aacaattgtt 5220 tagttggctc atatcgatat taacgaggcc gagaaacgtg tacaaagtaa aattgataat 5280 ggggaagatt ctgcagaaat tgagcatcag gtatgttgca ttagttgaaa ccatataaac 5340 gttggacgtg ttgtgctaga agatgtttct accaagctga agatactttt cggtggtttc 5400 cgctttagcg tatctttttt aaaataaaaa ataatcaaag tactgacatt ctggacctct 5460 agaaatttgt tttgaagaag catcttgagg cgttgccaga tgctcttgta cgtcttcgta 5520 atgcgacgaa cgacttggaa tccatttctt ctgacagtgc atacgaagga actcctgaac 5580 ttgaacaagc aaatgaatat ttagaaaaag caaaagaagt aattgaaaaa gagcaaacga 5640 atttccccac gaatgggtac cactgaatac acatttacta gcttttgtag atccctgcta 5700 aaatttatta ctgacccttc aaaagacgat catgaaaagg ttctacgttc atcattcttt 5760 acccaccctt tcaatgctat cctactcaac tgtactgtgt cttcgatttt tattccaatc 5820 cattaaaata atagacaatc ccaactactt aaggattatc caagtcatgc ttaaatcttt 5880 tttaagattt cgccaatcta ttctaatttt gccagttagg tgaaactgtt cacataaaaa 5940 aacatacgta ttccccttta ttctactaga gatttaaatt ttagctggac aatcaaacaa 6000 ttttaaaaca accaaatgtt cctgctaaca agcagcatag acagtcgtcc tctagtattt 6060 atttgcactt ttattgtgag tgtaatgtag cgtatcacta tgttagattg tacagctcat 6120 tgtttgacgt tacttaccat ctgagcaaag agaaagaaac acttcgcgtg aacattaaac 6180 agattgggag cataaaacgt tccatttgta tacctatggc aagtccgcca atattatcaa 6240 gggaatcacc tgtgttgggt ttagaagaag agaagaaatc tgacggcggc aactctgaag 6300 taaatattga cccttctgca tcttcatcgt tgaaggaaga tgtagatggc gaagagggag 6360 ctgatacaaa aatagatcca caccttcttg aagaagatga tttaaatcct gtagaggatt 6420 acgattctaa aaaccaaaaa gtcgttgaag catctaaact tgaccttgac gctacatcaa 6480 ctgtttgggc ggagagtctt agtattaata gagaagctag tatttcccaa tcaggcattg 6540 tagacaatga aatggagaaa aaagttgaag aggaagaaga gtttgacgaa tttgacgatt 6600 ttggtgaatt tgaacatgat atccccatct gtacatttat gggtgattgg caagatgatt 6660 tccaagatgc tgtcaattct gtatttggac tacctgaaac tccgaaaaca attgaagaat 6720 cctccttgga tccggatcgt atattgtctc tttggcaaaa actgattgaa atgcccgtgc 6780 tgcaacggcc agattggctg aggtctagca tcaggcatat ttttttagtt actatgggtc 6840 ttcctgttga tttggatgaa cttttgccta ctccttctac ccagggatca ttcaattctt 6900 cacgcacaat agaattgtct tccgtgacgt tgccaaaatc tgaggatgaa ccatatttag 6960 attattcagc tgctcgtcgt ttatgcagca taaacaaaga cgcattaacc caccgatctc 7020 atgagtcttt acaacagcat atagagttac tagaatcaac tctgcagcaa gctgttgaag 7080 ttgctcgata ttggaccgat aaaagggatt cagctcttag cgataagttg ttatatgaga 7140 cggttgtcga tgatttagtt caacatagca aacgtttaag aaactctggt cgtaaattcc 7200 tttctagaag gagcacttcc tctcataaat gatcccttta tatctttaat taccgacctc 7260 ttttcctgaa ttgctgtttt cttgtttaat tttacgggag taaaataaaa cggaagcgtt 7320 ttatttttgt tgctgacgaa cctttgttac gtctaacgtg attagatttg acttgtgatt 7380 cagctgtatt gtcttcttcg attcttttgc ctcttctctt ctttttaatg actaatattt 7440 taatgactct tctctatgtg tttatcaata ttatgtcaac cacttgtaaa tataaatgga 7500 acagtatcct aacctaagcg ccattcttgg ttatttacat aatagtccat aattatctct 7560 aaagaaaagg agcaaatttt gattcactgt tggtacttaa gttattcatc gttaaaaaaa 7620 cattaagaac atttgtcgac acatttctgg caaattgata acaacttgaa tgaactgtta 7680 aaattataag caggaagttg aaattttatt tttatttata cttattcgtt agacttgcat 7740 ttggaatgct ttggatttac tagtaccctt attctcttac aacctggacg ttcctagtaa 7800 tatcaaacaa ttaattgact tatggaatta gatggcttat cgactaaaaa aagctaaggc 7860 atttcttgac taaaaacgca aggaaaggac tataaaaatt tgattgttac tacaggtaaa 7920 tcgagaacca tcgtatatag gagctgcaaa attttgtata ttattggtat gtttgtcaga 7980 cgctaaatga ttcttacttg atggatagtt taaccttttt tttttgagat ttacatgcaa 8040 ttcatctatt gcctaaattc accagccatt attttgacaa atgcattaaa ctatttaagt 8100 atagcgattg cttttataaa aggattttgg tgcagtacta ggaatatgaa tacgcgattg 8160 atattgagta atgttctggc acaagcgttt tcagctgaca gatttaagca gtctgtttta 8220 taactattct caagtaatac tttcccattg aaaataaaaa gttatttttg taatgtggtt 8280 ggattgtata gtacgactct gcggttgaag aaatgcagaa agcatctttg gctagcaata 8340 taaataacaa aaaaaaaaaa aaattagtca agctaatttt gtgttttata aacaaaaaaa 8400 aaaaaccttt ggaacaaaat tcttagtata caaattgcga taacgtattg taatagccag 8460 cactcaattt ggattcttag tttcaagata tacatatatc gcttggatta acgctaacac 8520 cattttttac gattattttt cgcctgcaaa agaaaaaatc ataagggaaa tattatatat 8580 ttacaccata ccttcgcttt tgattcaatt agctttgata caaatacctg tcttataaaa 8640 ctggtgtttg ccctattgca acacctccat tcccttagta ctttttaaaa cgtttcatta 8700 tgactgccaa actcaattca gtaataatta atcccagcgt ggcgcatacg gccactgtga 8760 ttttccttca tggtatgaac gatgataatg ttgagacaaa catatcgtat attttcttca 8820 taatagtaac acatttatct taggtttagg agatagtgga caaggatggt cttttatggc 8880 aaatacgtgg tcgaatttta agcatattaa gtggatcttt cccaatgcgt aagtaaaaga 8940 atgtatttaa tcatgcaatt gtctaaagta atgtgtgatg aatgaatgag aattcgcatt 9000 acatactttg tattttgatg ctaattattt tgtatatagt ccatctatcc ctgttaccgt 9060 caacaatggc atgaaaatgc cagcatggta agtaattgtt ctgcttgatg ataaagtaca 9120 atgttcgcta aaactttttt aggtatgata tctacagttt tgcggatatg aagagggagg 9180 acgagaatgg gatactacgt tcagcaggcc aattgcatga attgattgat gcagaacttg 9240 ctttggggat tcctagtgat cgcatattaa ttggcggatt ttcacaaggt atgagctgcc 9300 atgaaagcgg ttaactcgct agtcattgca ttttcaaaaa aaaagtgaaa tggttttcac 9360 ttttcaaaag tatattgctt actaactcgt cattttctct aggttgtatg gtttcccttt 9420 acgctggact tacctatccc aaacgtttag caggaattat ggggcattca gggttcttac 9480 ctttggcttc aaaatttcca tccgctctct ctcgagttgc taaagagata cccattcttt 9540 tgacttatat gaccgaagat cctattgttc cctctgtctt gtcgagtgct tcagctaaat 9600 acctcatcaa caatctacag ttaaaatgtc tcgatcgacc ttttgaggga gacgcccact 9660 ctctctcttc agaatcgttt atggctatgt ataaatttac tcagacagtt attggttcac 9720 cataagtatt gtgtttttgg tgttgagact ttgcaaatga aggaaattga cgagtctttt 9780 gcacttttag agttactcat aaaattaaat aagatttacc gtgattaact tttccctttt 9840 ttagaaagca gtttcatact agtaacttaa gactatgaat tgtcatccca tttggtaggt 9900 tctctccaac agttttactc atttatggca ttttaaggat tgaagcattt tgttttgagt 9960 atagatacta tggagtttag ttttcatttt tagtagcaat atttcgcgac gcgttcattg 10020 tttacatgta gctgcatcaa gtataataca tgattatgag tgcatatact ctatagttga 10080 acttgtaaag taattaaact aaagaaacaa atagatctac tgtttttttg ttgtgattta 10140 gagaggtaat tttgggaatt tccgcaatat atatatcgtg gataactaca gcaattcttc 10200 tttctacata gtacgaacaa caaccaaaaa ccgtgtaatt tgattaatac acgttcaata 10260 taaaaataaa gtgcaatgga agaattcgca gaggggaatg atgatgaaca aatgatattt 10320 agtgacattg aagacctaac tgctcacggt aagcttgaat gagtatacgg cagttataag 10380 actcccagta tttgctttaa atatctacta acataatcaa agggattggt atgaccgata 10440 tcataaaatt gaagcaagca ggcgtttgta cggtgcaagg agtccatatg tccacaaaga 10500 gatttctcct taaaatcaaa ggtttcagcg aagccaaagt ggataaactg aaggaagcgg 10560 catcaaaaat gtgcccagca aacttttcca ctgcgatgga aatcagtcaa aatcgtaaaa 10620 aagtatggag tatttcgaca ggatccgagg cactcaatgg catccttgga gggggaattc 10680 agtcgatgag tattacagaa gtatttggag agtttcgttg tggtaaaaca caaatgtcgc 10740 atacattgtg tgtcactgcc cagcttcctc gagatatggg cggggccgaa ggaaaagtag 10800 cattcataga tacagaagga acatttcgac ctgatcgaat caaagccatt gcagaacgat 10860 ttggtgtaga cgccgatcag gctatggaga atatcattgt cagtagagca tacaactctg 10920 agcaacaaat ggaatacatc acaaaattag gaaccatttt tgctgaagat ggccaatacc 10980 gtttgttaat tgtcgattcc atcatggcac tatttcgggt tgactattcg ggtcgcgggg 11040 aattatcaga aaggcaacaa aaattaaaca ttatgcttgc tagattaaat catatttctg 11100 aagagtttaa cgtagcggtc tttgtcacaa atcaggttca agcagacccc ggagcagcaa 11160 tgatgtttgc gtccaacgat cgaaaacctg taggaggcca tgtcatggct cacgcaagcg 11220 cgacccgtct gctgttaaga aaaggtcgag gtgaggaacg tgtcgcaaaa ttgaatgatt 11280 ctccagatat gcctgaagcc gagtgcagtt atgtgattac acctggtggc atagcagacg 11340 tttcctaatt gattttctac ctattccaaa aaaaaaacaa aaaaagaaga gaaataagaa 11400 aaaagatcag ccaagttcag caatgccttt tatcattgcg tttgtgctta tgttttaaca 11460 aaattgagtg cactgattac gttaatgtta acattttact gctgcaatac tatatcttta 11520 atacaataat cagttcacta ttggtctcta aaatggcttt aaagaaaaag tttggatgag 11580 aagctcgcgc cattttagat aaccaaacca agattttctt aactacacat ttatcaaata 11640 gttgcgaatg gcgcaaaccc ccaaaagcaa cttattctta cgagtcaaca aatttgttta 11700 agtagtgtca tgtgaagttg ctatcgcatg tatcaattaa caaaagctga gcgcctttca 11760 ttgcaatcgt tggacaatct gacttccacc aaacaattat atcgtgtgag acagaattcg 11820 tcatcacctt tgttccagtt tatttcccga ttctatgaag gcctgcagat ttgcacgtgt 11880 tcaggcacct tcctttcgat gtttaccttt tgcgtcaata tgacaaaagg ggtacagttt 11940 ctcttgatcc gttgccaagg atgtgtttac cccggggctt tgtcgcaaca aacgagaaca 12000 gtccagcagc caggtttttc gataaccttc tgtcgtgccg ataaattacg atgcgcagtc 12060 gctaaattcg acatacagaa gcacagggca cgtttacgca gtgctacgaa attttactgt 12120 gggagcagtc ctttttctac catacgacgt tccgcaacct ttccagtgcc aaccaccacg 12180 atatactcac attttcgaac gtttggtaaa aatgtctact acttctcgtg aagatgctgt 12240 ttatttagcc aaacttgctg agcaagccga gcgctatgaa ggtatgtgct tttataaggt 12300 ttagagacgc gtagagcaga gatatatttg gtgttatttc tgcaagaaca aaattacaca 12360 catccaaggt tctatcggcg gaagcttttg agtaagcggg cttcctgtaa aacatcgatt 12420 gcatttattt ttgacatagt tgtgatcaga ggcttagttg gtagaatgtt tgtgtttgtg 12480 tgttgtatgg tggttgtcaa atgaactgtg cattggctat ggtgatgttg aaaaggtgtt 12540 ttagtatttc aacttgtttc ctaactttat ttctaattgt ttttactccg agaagaaatt 12600 tttttgttca tacagacata cggaaaatac atcatgctgc gcatctcgaa agtcgaaatc 12660 cttatgtact tatcactaat atatccaggc atggttgaaa acatgaaaag cgttgcctcc 12720 actgatcaag aattgaccgt cgaagagcgt aacttgctct ctgttgctta taagaatgta 12780 attggagctc gccgggcgtc ttggcgtatc gtttcctcca tcgagcaaaa ggaggagagc 12840 aaaggcaata ctgctcaagt tgaattaatt aaggaatatc gtcaaaagat tgaacaagaa 12900 cttgacacta tttgccaaga tatcttgacc gttcttgaga agcaccttat ccctaatgct 12960 gcctctgccg aatccaaggt tttctactac aagatgaagg gtgactacta tcgttacctt 13020 gctgagtttg ccgttggtga gaagcgccaa cactccgctg atcaatctct tgaaggttac 13080 aaggccgctt ctgaaattgc taccgccgaa cttgcaccta ctcatcctat ccgcttaggt 13140 cttgctctta atttctccgt tttctactat gagatcttga actcccccga ccgtgcttgc 13200 tacttagcca agcaagcctt tgatgaagct atctccgagt tggacagcct ttccgaggaa 13260 tcctacaaag attctacttt aatcatgcaa ttgttgcgtg acaaccttac cttatggact 13320 tctgatgctg aatacagtgc cgccgctgct ggtggtaaca ccgagggagc tcaagaaaac 13380 gctccttcca atgctccaga aggcgaagct gagcccaagg cggacgcata gatagctcgt 13440 tgctcatgat tgatgccata ctttcactac tatgaaccaa ttttaaaaaa tttatttgtc 13500 taagagttct ttttcgttta gatcttgtgt actacacgat ggaatagtct cattacattc 13560 tcgtatattt tcttctaatt tttttttctt tttgtctttc tttaaatttc tttttttttt 13620 acagtcgtag tatatgtgtg tatttggtag ctgctgtaaa aaatacagta gttggacgac 13680 attttaattt cgagtacaca acccgtaatg atttgcttgg ctaagtgggt gttgagttaa 13740 tcaagcaata gtctagatag atgtgagttt caaactgaag agttggtaga ttgagaatgt 13800 ttaagcctat cggattgaat ttgagctttt tgactgattt gcaccgaatt gcttaatatt 13860 ccttgcatac gaccgatgta ccacttttta cacaaagctt aaaaatttgt atgttctagt 13920 gatactcaaa cattattaaa tataaaagct ttaagtttgc atataaaacg cacagtaaac 13980 atttgaatcg atgcctgtga gaccatgcat acatttcacg cctccagaag gatttatgaa 14040 tgatcccaat ggtctggttt acagtaatgg aaaatggcat ttgttttttc aatggaatcc 14100 aacaggaaac caagccggta atcaacattg gggccatgca gtgagtaaaa acctgtataa 14160 atggaaatta ctacctaccg cccttgcacc tggagacgat catggattaa tgtttagcgg 14220 aagcgcagtg atcgataaaa ccaactcatc aggctttttt gaaagtggat ttttttcgag 14280 aaagtcggtt gaccctgaag aaagaattgt gttgatttat acaacgcatt atgataatcg 14340 tgaaactcaa aacattgcct actcattgga tggaggtatc acatttatca aatacaaaaa 14400 gaatcccatc ctcgacatca aggaatcgca gttccgcgac ccaaaagtgt tttggcatga 14460 agaaagtaga gcatggatca tggttgtagt gttagctcaa aaatacaaag ttctgtttta 14520 ccattcccta aaccttcgcg attgggtaaa actttcggaa tttggttctg caggcgtttt 14580 aggataccaa tacgaatgcc ctgactttgt tcgtctccct atcgagggta ccgacgagtt 14640 tcgatgggta ctgattgttt ccattaatcc tggttcttcg atcaatgggg gtagcatggt 14700 tcaatatttt attggagatt ttgatggtca aaccttcact ccaattgata gcgcgtccag 14760 aattttggat tgcggtcacg attgctatgc cactcaaacg tttggtaatg cacccgatgg 14820 aagagtcatt tccatctctt gggcaagtaa ctggaactat acgaacgatg ttcctatgcg 14880 tatgaaacat cgaggtatgt ttactattcc tcgagaactt actctttgct acacccatct 14940 caatcaagaa acgcgtggtc ttgtgctacg tcaacgtcca gttaatcttc accatcttta 15000 ctatccagat tctctaccag ctccactttt gttaaaccgt gtctgtgaat ttcctactgt 15060 ctggactagt gccactgtat tctacctcgc cgtttccatt ccgaaaagta ttgtattaga 15120 atcccctatg gagtttttat gcttgacatg gtctaccacg ccagatgttg agacctctaa 15180 ggagtatttt gaacttggat accgattcca cgacggtgcc gtctacgtcg aaaggggtgt 15240 atgctcgtct agttggaaat atcctttata tcctgaacgg tgtacgtctt ctgttcctcc 15300 ctcttcttac gaagataatt atatattgga aattgaagct gtagtagatc 15350 //