ID SPAC767 standard; DNA; FUN; 2525 BP. XX AC AL133442; XX SV AL133442.1 XX DT 10-DEC-1999 (Rel. 62, Created) DT 10-DEC-1999 (Rel. 62, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c767. XX KW Dynamin central region; dynamin family; Tf1-type LTR; KW vacuolar sorting protein. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-2525 RA Skelton J., Churcher C., Lyne M.H., Rajandream M.A., Barrell B.G., RA Quail M.; RT ; RL Submitted (03-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk. XX DR GOA; Q9URZ5; Q9URZ5. DR SWISS-PROT; Q9URZ5; VPS1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c767 is overlapped at the 5' end by cosmid c9G1, CC EMBL entry SPAC9G1, accession number Z98763 and at the 3' end CC by cosmid c17H9, EMBL entry SPAC17H9, accession number Z98597. XX FH Key Location/Qualifiers FH FT source 1..2525 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /map="IL" FT misc_feature 1..412 FT /note="nominal overlap with cosmid SPAC9G1 S. pombe FT chromosome 1" FT CDS join(complement(1544..1636),complement(1..1492)) FT /db_xref="GOA:Q9URZ5" FT /db_xref="SWISS-PROT:Q9URZ5" FT /label=SPAC767.01c FT /note="SPAC767.01c, len:528, SIMILARITY:Saccharomyces FT cerevisiae, VPS1_YEAST, vacuolar sorting protein 1., (704 FT aa), fasta scores: opt: 2031, E():0, (66.0% identity in 547 FT aa)" FT /partial FT /gene="SPAC767.01c" FT /gene="SPAC9G1.14c" FT /product="probable vacuolar sorting protein; dynamin FT family" FT /protein_id="CAB62830.1" FT /translation="MDPSLIKVVNQLQEAFSTVGVQNLIDLPQITVVRSQSSGKSSVLE FT NIVGRDFLPRGTGIVTRRPLVLQLINRPSASGKNEETTTDSDGKDQNNSSEWGEFLHLP FT GQKFFEFEKIREEIVRETEEKTGKNVGISSVPIYLRIYSPHVLTLTLVDLPGLTKVPVG FT DQPRDIEKQIREMVLKYISKNNAIILAVNAANTDLANSDGLKLAREVDPEGLRTIGVLT FT KVDLMDKGTDVVDILAGRVIPLRLGYVPVINRGQKDIEGKKSIRIALEAERNFFETHPS FT YGSKAQYCGTPFLARKLNMILMHHIRNTLPEIKVRINAALAKYQAELHSLGDTPVGDNS FT SIVLNLITDFCNEYRTVVDGRSEELSATELSGGARIAFVFHEIFSNGIQAIDPFDEVKD FT SDIRTILYNSSGPSPSLFMGTAAFEVIVKQQIKRLEDPSLKCVSLIYDELVRILNQLLQ FT RPIFKRYPLLKDEFYKVVIGFFRKCMQPTNTLVMDMVAMEGSYINTVHPDFLSGHQAMA FT IVQSQNSKPIP" FT misc_feature complement(11..889) FT /note="Match to PF01031 dynamin_2, Dynamin central region FT Score 414.87" FT misc_feature complement(890..1480) FT /note="Match to PF00350 dynamin, Dynamin family Score FT 347.16" FT misc_feature complement(1400..1429) FT /note="PS00410 Dynamin family signature" FT misc_feature complement(1493..1507) FT /note="ctaataaattgttag, splice branch and acceptor" FT misc_feature complement(1538..1543) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(1541..1627) FT /note="Match to PF00350 dynamin, Dynamin family Score FT 26.56" FT LTR 1932..2258 FT /note="Tf1-type LTR" FT misc_feature 2005..2525 FT /note="nominal overlap with cosmid SPAC17H9 S. pombe FT chromosome 1" XX SQ Sequence 2525 BP; 812 A; 478 C; 460 G; 775 T; 0 other; cgggtatcgg cttactattc tgactttgaa caatcgccat tgcttgatgt ccagataaaa 60 agtctggatg aacagtgttt atataagaac cttccatggc aaccatatcc attacaaggg 120 tattagtggg ttgcatacat tttctaaaga aaccaataac aaccttgtaa aattcatctt 180 tcaaaagagg ataacgttta aatatgggac gttgaagcaa ttgatttaaa atacggacaa 240 gttcgtcgta aatgagggaa acacacttca aggaaggatc ctccaaacgc ttaatttgtt 300 gcttgacaat aacttcaaat gcagcagtac ccataaataa actaggagaa gggccagatg 360 aattgtacaa aatggtgcga atatccgaat ccttaacttc atcaaatgga tcaattgctt 420 gaataccatt cgaaaaaatt tcatgaaata cgaacgcaat acgagctcct cctgaaagct 480 ccgtagcaga aagctcttca ctacggccgt ccacaactgt gcgatattcg ttgcaaaagt 540 cagtaattaa atttagtaca atactggagt tgtcgccaac aggagtgtcc cccaaagaat 600 gtagttcggc ctgatatttg gctaaagcag cgttaatacg aactttaatt tcgggaaggg 660 tgttccgaat atggtgcatc aatatcatat ttagtttacg agctagaaat ggagtaccgc 720 agtattgcgc tttagaacca taggaaggat gcgtttcaaa aaagttcctt tctgcctcaa 780 gagctatacg aatagacttc ttcccttcga tgtccttttg accgcgattt atgactggaa 840 catacccaag tctcaatgga ataacacgtc cagctagaat atctaccaca tccgtacctt 900 tatccattaa atcaaccttg gtcaatacac cgatagtgcg aagaccttcg gggtcaactt 960 ctctagcaag tttgagacca tctgagtttg ccaaatcagt attggcagcg tttacagcca 1020 atataatggc attgttttta gatatatact tcaaaaccat ttcacgaatt tgcttttcaa 1080 tatctcgagg ttggtcacca accggtacct ttgtaagacc aggtaaatct actagtgtca 1140 gagtaagcac atgaggagag taaatacgaa gatagatagg aaccgaagaa atgccaacat 1200 tctttcctgt tttttcttct gtttctctaa cgatttcttc acgaatcttt tcaaactcaa 1260 agaacttttg tccgggaaga tggaggaatt ctccccattc agatgaatta ttctggtcct 1320 ttccatctga atcagtagtt gtttcttcat tttttccact agcgctaggt ctattaatta 1380 actgcagaac caaaggacgc cttgttacaa tgccggttcc tctaggaagg aaatctctcc 1440 caacaatatt ttctaaaacc gatgatttac cgcttgactg tgaccgtacg acctaacaat 1500 ttattagtga atttttcaat ctaagttaaa attcacaaca tacagtaatc tgtggtaagt 1560 caatcaagtt ttgaacgcca actgtggaga aagcctcctg aagttgattg acaactttaa 1620 tcaatgaggg atccattatc aactatggat ccactaaata gacaatttta cgaatgggcg 1680 gttacaaacc tgatggtatg tgctgtggta gtgtagtgtt tactcggata tgtcgaataa 1740 aattagtacc tcgctaatcg gatttcagta aggctgcttg aaggtggctt aacaatatcg 1800 tgttgctaat tgatttacca aaaaagaaac agtgatgccc ttgaaacgtt gttgtcagac 1860 tttagacaat aaatctaatg tttaaccctc gataagtaat attaaagcga tgcatcattc 1920 catatttaac gaacgataat atactaatat tgcgttttac tacgttgcgt atcactatat 1980 gccctgtgtt ggaataacag gaaagatcct tttaatgata attgcgaaat gctatttatc 2040 ttaatacaaa gttacccatt gatacttgag tactgaaaaa attaacggaa actgctttaa 2100 ccttgttttt cagtacaatt ataagctatt ctgctgataa gtaaaaatag tacctattta 2160 cgagaatctt tataatatag tacgcgtttt gctgcgtacc gtaatattgt atatttcaaa 2220 taaaactacc accacagttt taaaaattgg ttacattacg cgtagctgac agtgctcaat 2280 acgctagttt attcaaaaac gagttttagt tcagtaggaa aagggaatgg actttataga 2340 aacagcaatt tttgctaatt attctctgtt ttgctgctga ttagaagtcg acaagtgtgt 2400 tcaatttgca catgccattg gacaaatttt agcaatcacc ttagcgtcaa acaatttatg 2460 ctttttgttg gaaaaaaacc aataacaatt gggacctttt tagcaataaa tggaagcgaa 2520 tttca 2525 //