ID SPAC732 standard; DNA; FUN; 5599 BP. XX AC AL133360; XX SV AL133360.1 XX DT 08-DEC-1999 (Rel. 62, Created) DT 08-DEC-1999 (Rel. 62, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c732. XX KW 6-phosphofructo-2-kinase domain; ATP synthase subunit C domain; KW fructose-2,6-bisphosphatase; PGAM; Vacuolar ATP synthase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-5599 RA Lyne M.H., Rajandream M.A., Barrell B.G., Zimmermann W., Wambutt R.; RT ; RL Submitted (03-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk and Agowa GmbH, Glienickerweg 185, D-12489 Berlin, RL Germany XX DR GOA; Q9URZ7; Q9URZ7. DR GOA; Q9URZ8; Q9URZ8. DR SPTREMBL; Q9URZ7; Q9URZ7. DR SPTREMBL; Q9URZ8; Q9URZ8. DR SWISS-PROT; O14127; YF51_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c732 is overlapped at the 3' end by cosmid SPAC3C7, CC accession number Z99568. XX FH Key Location/Qualifiers FH FT source 1..5599 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c732" FT /map="IL" FT CDS join(428..444,484..515,548..657,713..748,794..864, FT 1157..1379) FT /db_xref="GOA:Q9URZ8" FT /db_xref="SPTREMBL:Q9URZ8" FT /label=SPAC732.01 FT /note="SPAC732.01, len:162, SIMILARITY:Saccharomyces FT cerevisiae, VM11_YEAST, proteolipid protein vma11., (164 FT aa), fasta scores: opt: 680, E():0, (62.9% identity in 159 FT aa)" FT /gene="SPAC732.01" FT /product="Vacuolar ATP synthase" FT /protein_id="CAB62424.1" FT /translation="MSSNLCPIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGA FT FRPEIVMKSLIPVVMSGIIGVYGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTG FT VAAGYAIGVVGDRGVQSFMRQDRIFVSMVLILIFAEVLGLYGLIVGLILQTKTSNVCY" FT misc_feature 445..450 FT /note="gtaaag, splice donor sequence" FT misc_feature 464..661 FT /note="Pfam match to entry PF00137 ATP-synt_C, ATP synthase FT subunit C, score 43.50, E-value 4.9e-09" FT misc_feature 471..483 FT /note="ctaacgaactaag, splice branch and acceptor" FT misc_feature 516..521 FT /note="gtatgg, splice donor sequence" FT misc_feature 537..547 FT /note="ttaatagctag, splice branch and acceptor" FT misc_feature 658..663 FT /note="gtttgt, splice donor sequence" FT misc_feature 699..712 FT /note="ctaatattttgaag, splice branch and acceptor" FT misc_feature 749..754 FT /note="gtatgt, splice donor sequence" FT misc_feature 780..793 FT /note="ctgacgataaatag, splice branch and acceptor" FT misc_feature 865..870 FT /note="gtatgt, splice donor sequence" FT misc_feature 1145..1156 FT /note="ttaaccaagtag, splice branch and acceptor" FT misc_feature 1167..1349 FT /note="Match to PF00137 ATP-synt_C, ATP synthase subunit C FT Score 76.90" FT CDS complement(2071..3297) FT /db_xref="GOA:Q9URZ7" FT /db_xref="SPTREMBL:Q9URZ7" FT /label=SPAC732.02c FT /note="SPAC732.02c, len:408, SIMILARITY:Saccharomyces FT cerevisiae, F26_YEAST, fructose-2,6-bisphosphatase, (452 FT aa), fasta scores: opt: 1033, E():0, (44.2% identity in 412 FT aa)" FT /gene="SPAC732.02c" FT /product="probable fructose-2,6-bisphosphatase (EC FT 3.1.3.46)" FT /protein_id="CAB62425.1" FT /translation="MKDIEGLLGLCVCFVGLPASGKTSSAMKLSRYLTWMSVSTHLYDT FT KEIDNSLSENSNSDSENLAKTLSPIFDNLESVFKKGTDVAILDFNQCTLKFRKSIVELA FT KARNMLLMFVEVVCTNQKIIDENITDMCQHSPYFKSFPFEESKNKILDSIHEYEKHYTP FT LSEAEECTFVRIVDFGAELIVHKLENYLESRIVYYLSNLRTRRRSIWLSRHGESQFNVE FT GKIGGDSSLSPQGLKYAALLPEYVAKFSIGEKGLTVWTSSMARTIQTARHLNCQKLEWR FT ALDELDAGTCDGFTYDYIEQNFPHEAELRNNDKFHYRYRGGESYMDVVRRLEPIIMELE FT RQGDVFIICHQAILRCIYGYYHNLSLEELPFINVPLHTIIKLTPMTYETIEERVTVPIS FT AVSTQRGKH" FT misc_feature complement(2137..2670) FT /note="Match to PF00300 PGAM, Phosphoglycerate mutase FT family Score 184.46" FT misc_feature complement(2638..2667) FT /note="PS00175 Phosphoglycerate mutase family FT phosphohistidine signature" FT misc_feature complement(2680..3288) FT /note="Pfam match to entry PF01591 6PF2K, FT 6-phosphofructo-2-kinase, score 67.10, E-value 3.8e-16" FT misc_feature complement(3229..3252) FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(4626..5599) FT /codon_start=3 FT /db_xref="SWISS-PROT:O14127" FT /label=SPAC732.03c FT /note="SPAC732.03c, len:324, SIMILARITY:Schizosaccharomyces FT pombe, O60162, hypothetical 68.7 kd protein., (598 aa), FT fasta scores: opt: 677, E():0, (41.3% identity in 303 aa)" FT /partial FT /gene="SPAC732.03c" FT /gene="SPAC3C7.01c" FT /product="hypothetical transmembrane protein" FT /protein_id="CAB62426.1" FT /translation="VFGKHFQELANDYGDNLVVVNLLDQKGREAPLRSGFEKLCKRNKN FT PPLSYVYYDYHKQGSRNLPLFLAEIQSLLIEGKYYAEHGSKTTAMQTNFVRTNCMDCLD FT RTNVIQTSIAQFILNMQLHDIGVLSSSESLEEYDSFLQDFRLIWANTGDYISDLYTGTP FT ALKGDVTRHGTRTIFGAFKDLLNCFRRYILNNFFDGMLQDSYDLGLGVFRPYDSLSIPD FT LPLRFHWTRFVAPGIFLFTTIILTIQELFGNPSLFCRLLYSIPMVNAGIYLYFHRRQYV FT NWPRLVLPTYAKGGWFSFRNHFRNITLKVFRFLRSGSFKKSV" FT misc_feature 5344..5599 FT /note="nominal overlap with cosmid SPAC3C7 S. pombe FT chromosome 1" XX SQ Sequence 5599 BP; 1813 A; 923 C; 978 G; 1885 T; 0 other; gatcgtaagc aaaagaatga atgaattttt acatccatta tgttggcacg caaatgataa 60 ctaaatacga ataggagttg agaacttgtg ccaaaagtcc aaagtcatca atgtcttcca 120 tatatattat taagatgttt ctattggcat aatgttttta atcctattta caagttgtta 180 tgttacctgt ggaagcccgc acatgattta aagattacta gcgcattctt atatttttat 240 tcaggtattc agcatttgtt cctggcatac tgaagaccac ttcaatatta ccgtacccta 300 tctgcatctc tttaattcca ctaacaacga aattcaacga aatactacat cgacaaagtg 360 cttacaatca ctgcgttttc atatagctta gaacgtcccg tttgcataac gaacataact 420 aaacaatatg tcaagtaatt tatggtaaag gatatttttc tttttcattt ctaacgaact 480 aagtcctata tattcttctt tttttggttt tgcaggtatg gacgattgaa ttatgattaa 540 tagctaggtg tttgtgcctc aatggttttt tcatgcctcg gagcaggcta tggaacagca 600 ttggctggaa gagggatagc tgccgtaggt gcttttcgtc ctgagattgt tatgaaggtt 660 tgtgctgctg ttttgaacaa aaaataatga caacatcact aatattttga agtctttgat 720 tccagttgtt atgagcggta ttatcggcgt atgtaaaaga ttcaaaatct tgtaattcac 780 tgacgataaa taggtctatg gtttggtaat gtcggtattg attgctggtg atatgtctcc 840 cgataatgat tattccctat tcaggtatgt taaataagct ttgtgtgaag tttcaacata 900 attggatcta ttcatgcctt aatcttcatc ttgtattttg caaagattgt aattctttgg 960 actatacgtc tactaaagtg gttgtttgtc taccgacata cattcttttt tttttatgta 1020 catgttactt tccattgtag agttttgttt ttttcaaaga gcttttacaa atggagttag 1080 tgagatgatc catcatttga agcttcaata gtatttgact tttcccaatt agttttgatt 1140 tttattaacc aagtagcgga tttattcatc tctctgctgg attggcagtt ggtttgacgg 1200 gtgtggctgc tggttacgct ataggagttg ttggtgatcg tggtgttcaa agttttatga 1260 gacaagatcg catatttgtt agtatggttc ttattttaat tttcgctgaa gttttgggat 1320 tgtatggact tattgttggc cttattttac aaacaaagac ttcaaacgtt tgttattagt 1380 aatcgagtat gtcaagggtt ttattctgtc ctgtcattag ctgaagatga taggatttat 1440 ttaatgactt taaattgaag aagtctgcta aaaaatatga tataaaagcg acgtttaata 1500 tcaaatactt ttatcttgtt gactttctct aaggctaatc aaaaagtact ctaactacta 1560 atactttgtt cgctattttg tgagtttttc gatatcttta gaattatgga tttgctatcc 1620 ttccttgctt aattatttgg aaagtatata ccgtataact ttgattgatc tcgagagcaa 1680 cagccacagt tttgtaaaac gtggttgaag ctcgattcta gaaaaaaaaa agataatttt 1740 gccattcatg tcttaaatgc ttcatagtgc attaaagata acaactttat gaatgacata 1800 cacaaccaat tgatggttcc gagtaaacta cttttttctt tctattttcg tttaaaagat 1860 ttgttcacgt acagtaaata ctttcggcta tctgcatagt gatacgaact ttaggtaact 1920 ggaagaataa ttaacaataa actttgaaaa aaataatttt gtattgaagt catcaatgct 1980 gcattttatt attaaaaaat aattcacaac ctttgaaaaa ttctgatcaa cgttaaagga 2040 ataaatcagc acattatgaa gaattttctt tcaatgtttg ccgcgttgag tagaaacagc 2100 agaaatgggg actgtaacac gctcctctat cgtttcgtat gtcattggag tgagtttaat 2160 tattgtatgt aaaggaacat taataaatgg taattcctca agtgaaagat tatgatagta 2220 tccataaata caacgaagaa tagcttgatg acatataata aaaacatctc cctgtcgttc 2280 taactccata ataatcggct ctaaccgcct tactacgtcc atataagatt ctccgccacg 2340 atatctgtaa tgaaatttat cattatttcg caattcggct tcatgaggaa aattctgttc 2400 aatataatca taagtaaaac catcacacgt tcctgcatct aattcatcca gtgctctcca 2460 ttcgagcttt tggcaattaa ggtgtcgagc agtttggata gtcctagcca ttgaagatgt 2520 ccaaaccgta agtccttttt cacctatcga aaacttagca acatattcag gaagaagagc 2580 agcatatttt agtccttgag gacttaagct tgagtcacca cctatttttc cttcaacatt 2640 aaattgactt tcaccatgtc ggcttagcca aatacttcgt cgacgcgtac gcaaattaga 2700 gagataataa acaatcctag attcaaggta attttcaagt ttatgaacta tcagttcggc 2760 tccaaagtcc acaattctaa cgaaagtaca ttcttcagcc tcggacaaag gtgtgtagtg 2820 tttctcatac tcatgaatac tatcaaggat tttattcttc gattcttcga acggaaagct 2880 tttgaaatat ggtgaatgct gacacatatc agtaatgttt tcatcgataa ttttttgatt 2940 tgtgcagaca acctcgacaa acattaagag catattccgc gcttttgcca actccactat 3000 acttttccga aactttagtg tacattggtt aaaatcaaga atcgcaacat cagtaccctt 3060 tttaaaaacg ctctccagat tatcgaatat ggggctaaga gttttagcta aattctctga 3120 atcactgtta gaattttcgg acaacgaatt atcaatttct ttcgtatcat aaaggtgagt 3180 agaaacggac atccatgtca aatatctgga taatttcatt gcagaagatg tcttgccaga 3240 tgcgggtaac cccacaaagc aaacacatag gcccaaaagt ccttcaatgt ctttcatgtt 3300 cgatcttaat gtaaaaaagt tagttcttaa tttaaatttt gggtgtggtt atgttgcaaa 3360 caaatattaa agaataaatc ataagaatat gaccaggttg ctaatcctgc cagaatcggg 3420 ttgaattatt aagtgaatat gttaaataat gaatatatgt taaagagtcg tatttcgctc 3480 gaaatgtatg attcgccttg aagagcttga tggacggttt gcagtactcg tacttatgtt 3540 aagtatcctt agagtatgta gtgaactcgt caatgcgtta aaaagctata atttttaaaa 3600 agaaaaacga ttaaattaat aatatttaac agaacggctt aaaatataca agttcagaac 3660 attgcactcc tgctaacata aacaagaggg gtatacgtgt aagttcaagc ttaatatact 3720 aaattgcagg tcttcgttac gcagtattat tgcgagagat gtttacgctt ccgtaagcta 3780 cattaagtga tctcaagtct ttgacttggt actaggagta gttaaatcca ctattaaaat 3840 aatattcaaa attaagtgtg agcatttcca tttctataag cataattatg ctaagctgat 3900 tgtaaaagtg tatataatca ttttcgtaat attgtggagt tttcatacaa aaactgtcgg 3960 gtaaggcata acttcatcat tttttcagta atgtgaattg tgaattaaga ttctggattg 4020 gaagtattag aaaacgttgc tgaactgtat gatcttttaa aaaattcatt tttataacat 4080 gttcattgaa aatattaaac cagaatgccc ctaaattgga agaagtatgt atcctaaata 4140 ttgaacatga acaacactta tgaaattgaa agaaagcttg gctttttcat atatttcatt 4200 tactgtttaa aaccgagtaa aacttgagtc aaaagcctca gtgattcaat ggtcaaagtt 4260 gcccaatatt taacactgac ttaataaaag ttagaaatat taattttgtc tgctatattt 4320 ttttcgtgtg ttatccatac tacaatagtt tgttaagggc tctaacaatt atgaaaatct 4380 taaattacta attgatattg gtaggaagct tggagcaatt aaagaaaata aagttacaat 4440 taccaatcac gtaaaatgat ctgaattagt cacttgataa agttgggtag catcgctttg 4500 ttcagtagca agattgacat ctatgaagtc taatgaaata aagagtgaac ttcgagtgtt 4560 caaagagcgt tttctgctaa ttaagagtta tagcaatact gttctttagt tattgaaatg 4620 cacagttaga cacttttttt gaaagagccg gaacgcaaaa aacgaaacac tttaagagta 4680 atatttcgga aatgattacg aaacgaaaac caacctccct tagcatacgt tggtagtact 4740 agacgaggcc aattaacgta ttgtctacga tgaaaatata ggtagattcc agcattcacc 4800 atagggatag aatataacag acgacagaac agacttggat ttccaaataa ttcttgtata 4860 gttaatatta tcgtggtgaa caaaaaaatt ccaggtgcca caaatctagt ccagtgaaaa 4920 cgcaaaggca agtccggaat cgaaagtgag tcgtaaggac gaaatactcc aagacccaaa 4980 tcatagctat cttgcagcat accgtcaaaa aaattgttaa gaatatatct acgaaagcag 5040 tttaataaat ctttgaaagc tccaaaaatg gttctcgttc catggcgagt cacatcgcct 5100 tttaaggccg gggtaccagt gtagagatca ctgatataat ctccagtatt agcccatatt 5160 aagcgaaaat cttgtaaaaa actatcatat tcttccaaac tctcagagct agaaagtaca 5220 ccaatatcgt gtaattgcat gtttagaata aactgtgcaa tgctagtctg aatgacgttc 5280 gtccgatcta agcaatccat acaatttgtc cttacaaagt tggtttgcat tgcagtagtt 5340 ttagatccat gctcggcata atattttcct tctatcaaga gactttgaat ttcagcaaga 5400 aacaaaggga gatttctact tccttgctta tgataatcgt agtaaacata agacaaagga 5460 gggtttttat ttcgtttaca taatttttca aacccagacc gtagaggtgc ttcacgtcct 5520 ttttggtcta gaagattgac caccacaaga ttgtccccat aatcgttggc aagttcttga 5580 aaatgcttcc caaacacag 5599 //