ID SPAC631 standard; DNA; FUN; 8661 BP. XX AC AL391713; XX SV AL391713.1 XX DT 29-AUG-2000 (Rel. 64, Created) DT 29-AUG-2000 (Rel. 64, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c631. XX KW bromodomain; f-actin capping protein beta subunit. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-8661 RA Wood V., Rajandream M.A., Barrell B.G., Seeger K., Harris D.; RT ; RL Submitted (24-AUG-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9HGP5; Q9HGP5. DR SPTREMBL; Q9HGP3; Q9HGP3. DR SPTREMBL; Q9HGP4; Q9HGP4. DR SWISS-PROT; Q9HGP5; CAPB_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c631 is overlapped at the 5' end by cosmid c25A8, CC EMBL entry SPAC25A8, accession number AL021809. XX FH Key Location/Qualifiers FH FT source 1..8661 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c631" FT /map="IL" FT misc_feature 1..106 FT /note="nominal overlap with SPAC25A8 S. pombe chromosome 1" FT CDS join(complement(1137..1142),complement(543..939), FT complement(90..493)) FT /db_xref="GOA:Q9HGP5" FT /db_xref="SWISS-PROT:Q9HGP5" FT /label=SPAC631.01c FT /note="SPAC631.01c, len:269, SIMILARITY:Gallus gallus, FT CAPB_CHICK, f-actin capping protein beta subunit isoforms 1 FT and 2, (277 aa), fasta scores: opt: 858, E():0, (51.1% FT identity in 266 aa)" FT /gene="SPAC631.01c" FT /product="putative f-actin capping protein beta subunit" FT /protein_id="CAC05483.1" FT /translation="MNSEDAALDLLRRLNPKDISKNLDTILSVAPDLADVLLSSVDQPL FT KVNTCSESGNQYLLCDFNRDGDSYRSPWSNKYDPPLEDGLVSTDRVRKLEVSLNEAIRV FT YLDLYYEGGVSSVYLWDQDDSYAGAVLIKKASTSNSSGWDSIHVFECLPTTETNVYDYR FT LTSTIILFLSSGSEEQSALPSKALNLSGHLTRQTSQRLPAADDDTEIANVGKLVEEMET FT RMRNFLQDVYFGKTKDIINQTRSIQPVSDAQPNDSALRSVLNDLSI" FT misc_feature join(complement(543..933),complement(105..493)) FT /note="Match to PF01115 F_actin_cap_B, F-actin capping FT protein, beta subunit Score 390.29" FT misc_feature complement(494..507) FT /note="ctaacttcatttag, splice branch and acceptor" FT misc_feature complement(537..542) FT /note="gtattt, splice donor sequence" FT misc_feature complement(940..956) FT /note="ctaacttattcttttag, splice branch and acceptor" FT misc_feature complement(1131..1136) FT /note="gtaagt, splice donor sequence" FT CDS 3833..6016 FT /db_xref="SPTREMBL:Q9HGP4" FT /note="SPAC631.02, len:728; simialr to yeast BDF1" FT /gene="SPAC631.02" FT /product="protein with 2 bromodomains, putative involvement FT with sporulation" FT /protein_id="CAC05484.1" FT /translation="MTPVQVPNLDVSVTSSLITTAPVTGNAATISTFTPGSPEPSMNGE FT EKESSYFPISENDDGTLDLFGDSELEKEQKGDNQETDYSSQYLHPTPPYTNFDDESPSS FT PTHPSVSNITVDGDSKKHSLQLQEEEKSSESLDSHTHPPKRVRNEDDSLTFSKTSPVSP FT SSLKDGASNTVTNDASNKIKSEASESASPSALQALDSTAAGSSKEHSSPHDETVKKEEN FT DKDQYPPMTKEQHKYIHAMLRQLRRGRDSIPFRAPVDPVKQNIPDYPTIIKNPIDLGTM FT QKKFSSGVYSSAQHFIDDMNLMFSNCFLYNGTESPVGVMGKNLQATFERQLKQLPSAYV FT TSYSRPGRRPRSMTAPKGGARTRRQAAMYSNSSSGIRETMYDLKPHRRKDAAEMKFCQS FT VLKELLKKQHEAYAYPFYKPVNPTACGCPDYFKVIKHPMDLGTMQNKLNHNEYASMKAF FT EADMVLMFKNCYKFNSAGTPVHLMGKKLESIFQKLWANKPDFDSETYMGMSSVNTDYYY FT GDNEVFDSGDEFLEDDGEEFEAVNRQIHKLQSTLQAMKSRARSSSVSRRSRSRSLSVDI FT YPPITYEMQNELAEQCNYLSADQLSHVAEILRAALPHLRNTDEIEIDVSAMPPDVFYKV FT YYYVCKGDEIGAEALATASHTHQEKKKGRALSETEQAEKIRQLRAQLDRFSGIAQNKNT FT VTGNIAAYNTKSLGSDDSSSEDDGESSESSDSA" FT misc_feature 4535..4798 FT /note="Match to PF00439 bromodomain, Bromodomain Score FT 124.15" FT misc_feature 5018..5287 FT /note="Match to PF00439 bromodomain, Bromodomain Score FT 141.16" FT CDS 7326..8661 FT /db_xref="SPTREMBL:Q9HGP3" FT /label=SPAC631.03 FT /note="SPAC631.03, SIMILARITY:Saccharomyces cerevisiae, FT Q04177, d9509.17p., (643 aa), fasta scores: opt: 165, FT E():0.0019, (23.5% identity in 438 aa)" FT /partial FT /gene="SPAC631.03" FT /product="hypothetical protein" FT /protein_id="CAC05485.1" FT /translation="MAVRSRPQLAFQTEKENGIGITAFNETAKLYANVVEALDAQRLRI FT FDSVAGSLKTEYILEKEKVISCIAWEQKPLYASEQITTDISGSGEILVLGTNSGEILIY FT SEHLGSLTRTYSFGILQKIIGAHVLANDGFAIDITGKVVCFSVNTGEVRTSFTVPSSSR FT EFSGLYLSTVFKNLALASSHNIHIVDLNHRNPIDSLTTHTSMINSVVFQYLKDENKFYF FT GVSANQDRFINLYSKELDGTGEFPTNTVKNVGALVCENEVKMLSIAYEIENPETGVLAA FT LTNDGTIEMFENPWLSKLRQNGTNASSLSHRRKLLTSHSTLKICFCRSRGDPPIVLESI FT AFESTDSLTVVWKESTRTVFETVPWRILSSQATNGLIELVRSKTRLTSKNKTVSNVYDE FT SNATISSGVMQKDLRKTEEIGSAEAMEGEEQEPSLAERLQNLTKLD" XX SQ Sequence 8661 BP; 2636 A; 1521 C; 1650 G; 2854 T; 0 other; tttatattag tcaatctttc tatgactatt ttcgttgaag atggaacgaa tactatgaga 60 agatcacgga aagaaaacaa aaagcaatcc taaatggaaa gatcgtttaa aactgaacgc 120 aaagccgaat catttggttg ggcatcggaa acgggttgaa tggaacgagt ctggttgatg 180 atatctttag tttttccaaa gtagacatcc tggaggaagt tgcgcatccg agtttccatt 240 tcctcaacca atttgccaac attagcgatc tcagtgtcat catctgccgc tggtaatcgt 300 tgagaagttt ggcgagtcaa atgacctgaa aggttaagag ctttggaagg caaggcagac 360 tgttcctccg agccagatga aaggaaaagg ataatagtac ttgtaagtcg gtaatcataa 420 acgttggttt ccgtggtagg cagacattca aacacgtgaa tagaatccca tccgcttgag 480 ttagaagtag aagctaaatg aagttagaat tatataatga aattgacaga aaataaaaat 540 accctttttt atgagaacgg cgcctgcgta actatcgtct tggtcccaaa gataaacgct 600 actaacaccg ccctcgtaat aaagatcaag atagacacgg attgcttcat tcaaagaaac 660 ttctaattta cgaactcgat cagtagaaac taaaccatcc tccaaaggag gatcatattt 720 attgctccat ggtgagcggt acgaatctcc atccctgtta aagtcacata acaaatattg 780 attacctgac tcagaacaag tatttacttt tagaggctga tcaacagacg agagaaggac 840 atcagctaaa tcgggagcga cggaaagaat tgtgtctagg tttttagaga tatcttttgg 900 gtttaatcgt cggagtaaat ctaatgcagc gtcttcagac taaaagaata agttagctga 960 tattgtaaag aaggataacg acaaagaatg tgaaaagcaa attcataaag tctctgagca 1020 atactgacga aaatcgatga aatatgcatt caatgataag atgaatttac agatggctat 1080 ataataaaaa tataacaaaa aaattgggca aggtaattaa tttcagtaat acttacattc 1140 atggtgagtt gatgatataa tgctttaaac aaaagaatac aaccggatag cgatctcaga 1200 ataaaagtga ggccttaatg attataggaa gtaactttag attcgtcgca taaattacga 1260 agattcttca cggtgcagta cacaaatagt agaggtgact gactggtggt gaggtacttg 1320 tactctggtg ctttctgcgt aagtttagaa agtgaacaac atatggcggt gaacattgaa 1380 cgatgaaccc tctggtgatg acaacgtagc aaaaaccaac ctcaaactta ctttataatc 1440 aaactggtaa tatcaatgag ctgttaaaaa taatcaaaag aagagcacat tttgttcaag 1500 cggaaaaagt caagaattta ttagatgcta ctgttggtca cttcaataag ctgtctattt 1560 ttcaatattg aacattttca agttaaattt agtattggca acgaatacaa aacttttctg 1620 tatatcggtt aaattcctta atacagaaac aaggagaatt atatgccaaa gtcaatgctt 1680 gaatcgcttt ctatctaaag tttggatttt ctacgagtca aaccatgtaa agtcataaaa 1740 aagtcaataa caaacgtcct tctttcattt taagcaatgg tctttcattt tatttaaact 1800 atggatcttt taaaatctat tcttatgttt ataaccttaa caaatgcatt gtttaactgc 1860 tttttgatct ttatctacat ctcaccttct gttttagcat ccactcaacc tttaataacg 1920 ttttactgtc gttatgcagt atgacaactt acgaatttag tgttcgtata tcttcaaact 1980 gcgttcctta gaaataaaga acatttaatt tgttttgtta aacggttttc aaatggaata 2040 gcgcgactca acgttctaaa aatttgcgga acattaactg tgatcaaaaa ttttgggaga 2100 gcctagattt tgccactgtc acgtaattat gtacaagcgt tcaactccca tctctcaccg 2160 atttctgatt cgcaaaaatc acatgggttt tttcatggtg tggtcctttt aaccaatcag 2220 attgtaggac ggtttctcaa atgaccgaga aatgattcca ttccgttcca taagattctt 2280 tgtcccgttc ggagttcttg aaatcccgag ttcgaatctt gcattctaac ttttttcgta 2340 tgtttggcca tactaagact actagcgcac ggctgaaacc aattgtgtaa acaaacattt 2400 ctaaccttgc ttaaactgga ttgaaccttt ttgtcagtac attggaggat tacattggat 2460 aattgcactt ttccgtattt ctatctgttt attgtcttga caaaggctgt aattactgtt 2520 gttgtagcag tgagtgtgtg attacaaaca cttaaaacag aaaatgtgct ttgtgggttt 2580 gaaactttta tcgaaatttt gtaagaagtt aacctgcaat tattgttgat catttatctt 2640 ttaattgtat tattattaag ttgtttactt gcagaacaca tagtttggcc gaattttttg 2700 gttttgtttg tttacaataa caccccgcga tttgcgttgc gcctgatagc tttagccagt 2760 ggtgacgtag gagaaagata attggtcagt cgccctccat tgaaattatc atctaatcgt 2820 tcatccgccg caatgttgat tcacatcaaa accctaaccc tgaggacaac ggtgacttat 2880 accaacatgt ttcctccttc cctctgcaaa ccacattttt cctcggttcg actcttgaaa 2940 ttgctagtcg cgcggaagag ttcgatatcg ttattaatcg ttaatttgtt atagggcact 3000 acttttttgt ccctttgaag ccaagtcgct ataaatctta agtcatttgt gctatctgtt 3060 attgtttggc gctatacaac gaagtaaata ttgcatattg cttgttttag tgtaataggc 3120 agtttatgcg cgcgtgtgtt gtgaaggtta gccggcatta attttgttca ttcttgtttt 3180 tgtgtactat tagcaatttg atattcagtg ttgctgtgtt gattgctgta gcttatacgt 3240 caacgtggca attttgtttt gtaagtagtt ttgtttgtgt ttatgacagg tgaagtgggt 3300 gaggcagtga gggcagtcca actcttaatt tggatttttt gctggtgatt taggattttg 3360 atatggtttg tcgaagtttg ttagaattgt cgctttttta tcctaaaggt taaaaaattg 3420 gaattggtag tctcttcatt gaaaggaact gactttaaaa tttttttttt gttttgccaa 3480 agatacgttt cttttctgtg ttgcgacgcg tctaccacca ttatattgta gactgtggaa 3540 aggtggtgct attgttcctc ttttgttaat atctcgtgtt ccgtttgaca atgttaccta 3600 cgcctttttt tatcgtagtt ccttttattt atataactaa tgatggctta tgtcagtagt 3660 tatttttcac cgtcgttaat ttttgtcgtt gtaaatatgc catctgtttt ttcccgcttc 3720 actttttttg gactttccta acaattttta agatgttaat attaactttt ttagggtgaa 3780 ggaggcgttc acagatatta ttactgtcct caataagctt gaagatctcg atatgacccc 3840 cgttcaagta ccaaatttag acgtttcggt cacttcatct ctcattacta ctgcgcctgt 3900 aactggcaac gctgctacca tttccacatt tactcctgga tcacccgagc cttctatgaa 3960 tggtgaagaa aaggaatcat cttattttcc aattagcgaa aatgatgatg gaaccttaga 4020 tctttttggt gactcagagt tggagaagga gcagaaaggc gataatcagg agactgatta 4080 ctcttctcaa tatttgcatc cgactccgcc ttatactaat ttcgacgatg agtctccatc 4140 atcccccact catccctccg tgtcaaatat cactgttgac ggtgactcta aaaaacattc 4200 tttgcagctg caggaggagg aaaaatcctc tgaatcgttg gattctcata ctcatcctcc 4260 taagcgtgta cgcaatgaag atgactcctt aacattttca aagacatcac ccgtatcacc 4320 atcgtcgctg aaagatggtg cctcaaacac cgttaccaac gatgcgtcta ataaaattaa 4380 atctgaagct tcagaatcgg catctccttc tgctttacag gctctggact ctactgctgc 4440 tggcagcagt aaggagcata gttcccctca cgatgagact gtaaaaaaag aagaaaatga 4500 taaggatcag tatccaccaa tgacaaaaga gcagcataaa tatattcatg ccatgcttcg 4560 ccaactacga cgtggtcgtg actccattcc gtttcgtgct cctgttgatc ctgtgaaaca 4620 aaatattcca gattatccta ccattattaa aaacccaatt gatcttggaa ccatgcagaa 4680 gaagttttct tctggcgttt atagcagtgc acaacatttc attgatgata tgaacttgat 4740 gttcagtaat tgtttcctgt acaatggtac tgagtcacct gtgggtgtaa tgggtaaaaa 4800 tctacaagct actttcgaac gacaattgaa acaactacct tctgcttatg tgaccagtta 4860 ttcccgtcct ggccgtcgtc cacgtagcat gactgcacct aaaggtgggg cgcgtacacg 4920 tcgacaagcc gccatgtatt ctaattcttc atcaggaatt cgtgagacca tgtatgattt 4980 gaagccacac cgtcgcaaag atgcagctga aatgaagttc tgccaatctg ttcttaaaga 5040 attgttgaag aagcagcacg aagcttatgc ttatcctttt tataaacctg ttaatcctac 5100 agcatgcggt tgtcctgatt acttcaaagt aattaaacat cctatggatt tgggtacgat 5160 gcaaaacaaa ttgaatcata acgaatatgc ttcaatgaaa gcttttgaag ccgacatggt 5220 tctcatgttt aaaaattgtt ataaatttaa ttctgctggt actcctgtgc accttatggg 5280 aaagaagctt gaatctatct ttcagaaatt atgggctaat aaacccgact ttgattcgga 5340 gacctatatg ggtatgtctt cagtgaacac tgattattat tacggagata atgaagtttt 5400 tgatagcggt gacgaatttt tggaggatga tggtgaggag tttgaagcag ttaatcgtca 5460 aattcacaag ctgcaaagta ctttacaggc catgaaatct agagcacgtt cttcttcagt 5520 atctcgccgt tcacgttcta ggagtcttag cgttgacatt tacccaccta ttacatatga 5580 aatgcagaat gagctggctg aacagtgtaa ttacttatct gcagaccaac tttcgcatgt 5640 tgccgaaata ttaagggccg cactacctca tttacgtaat accgatgaga ttgaaattga 5700 tgtgtcggca atgccacctg atgtatttta taaagtctat tattatgtgt gcaaaggtga 5760 tgagatcgga gctgaagctt tagctaccgc ctctcatacc catcaagaga aaaagaaagg 5820 acgagccctg tctgaaacgg agcaggctga aaaaatccgg cagcttcgtg cccaattaga 5880 cagattttct ggcattgctc aaaataagaa tactgttaca ggaaatattg ctgcttataa 5940 tactaaatcg ttaggcagtg atgattcttc ctctgaagat gatggtgaaa gcagtgaaag 6000 tagcgattcc gcatagatgt ggttagcagg tttaaccaaa ttctttgatt tattttttta 6060 aggctctttt tattgttccc cttttttatc tttcctgtac atatgcaaat gcatacaaat 6120 tttctctatg gttttatact tcacactctt tattattttc atttttctat tttattgctt 6180 gcatgaactt attttttcca aataacctta gaagttcttt tttagtcttc ctgtataggt 6240 tttgttatga ttcttttgac tttgttaatg ttaaacacgg ttccattgct tgcttttatt 6300 gatgactgat tccaaagtta attgaaaagc aacaaatttg gacctatact tttttgtaca 6360 ttagttatag cgtctgtcta tagaaaatat aaaaattaaa tattttacta aattttttta 6420 atgatcgcgg tgctaaattg taagcaaaaa tttgattctt cctggacatg tgaatgtttc 6480 gttattgtac ttgaattggt agacgcgagt tataaaaatt tgaatacatt tttaccggca 6540 ttcctgtgcc taataacgcc atttacggtt gaaagataag cacaagcatt caagagtgga 6600 agggataaca atcaatgatt ttgaatctaa atgtttttaa gagcttagcg agttttggaa 6660 acgtcggaaa attgtagctt gctttgttgt agagaaagcc cgtttccatt ttattattgg 6720 tacgttttgt acttagagaa ctattgtttt acgatatcat ctcctaattg tatgttttaa 6780 ttgtatgcca ataacgtgaa tgtaggccaa atagccatac attcaatttt accgacttta 6840 actttaaata tcatatttac ttaataaata gtattttaaa catatatata tatatatata 6900 tattgaatta ttaaaagcag aattagcgtt ggtataataa attttcagca gttcaagcta 6960 tactgagggg taaaagctaa acaagctttt cttgagtagt agtttttcta aaaaggacac 7020 gggttttttc ttattcaaat cgtaaacatc aattgaaaag tcagcaggta aatattgaat 7080 gaatcgagcg acagagtata gtgttgaagc ttataaacat aaattagtgt agcaaagtat 7140 aatgaccttg ctgttaaaga agtttcacaa taatccgtac tatagtgtga gcgtacgcag 7200 catatatttt agattagtta atgagacata acgcgaaaat agaaaatttc gttacagtgt 7260 taagttttgt ttgcttaaaa cagtacaaac agcatacttg cttttctaac agcgtttgca 7320 tttcaatggc agtaaggagt cggcctcaat tggcatttca aactgaaaaa gaaaatggaa 7380 taggaataac ggcttttaat gaaaccgcaa agctttatgc caacgttgtt gaagcattag 7440 atgcacaaag gttgcgtata tttgattccg tcgctggatc tttaaaaacc gaatacattt 7500 tagagaaaga gaaagtgatt agctgcatag cttgggaaca aaagcctcta tatgctagtg 7560 aacaaattac aactgatata agtggttctg gagaaatttt agttcttggc actaattctg 7620 gtgaaattct tatatattca gaacatttag gatctttaac tagaacttac tcgtttggaa 7680 tattgcaaaa aattatagga gcacatgttt tagccaacga tggatttgcg attgatatta 7740 ctgggaaagt tgtatgcttt agtgtaaata cgggagaggt tagaacgtct tttacagttc 7800 cttcttcttc cagggaattc tctggtcttt acttatcaac ggtttttaaa aatttagcgt 7860 tagcttcgtc tcacaacatt cacatcgttg accttaatca cagaaatccg attgattctt 7920 tgaccacaca tacttctatg ataaattcag ttgtatttca gtacttaaaa gatgaaaata 7980 aattttattt tggggtttca gcaaatcaag acaggtttat taatttgtac agcaaggaac 8040 ttgatggcac tggcgaattt cctaccaata ctgtcaagaa tgttggagct ttggtttgtg 8100 agaatgaagt caaaatgctt tcaatagcct atgagataga aaaccctgaa accggtgtat 8160 tagcagctct tacgaatgat ggtaccatag agatgtttga aaacccttgg ctatctaaat 8220 taagacagaa tggtacgaat gcctcgtctt tatcacatcg acgtaaactt ctcaccagtc 8280 attctacttt gaagatttgt ttttgtcgtt ctcgaggaga tcctccaatt gttcttgaat 8340 cgattgcttt cgaaagcact gattccctca ctgttgtatg gaaggaatct actagaactg 8400 ttttcgaaac tgtcccttgg cgtatattgt catctcaagc tacgaatggg ctgatagaat 8460 tggttcgttc aaaaactcga cttacatcga aaaacaaaac tgtttcgaat gtttacgatg 8520 aatccaatgc aactatttcc tctggggtta tgcagaaaga tttaagaaaa actgaagaga 8580 taggaagcgc cgaagctatg gaaggggaag agcaggaacc aagtttagca gagcgcttgc 8640 aaaatttaac caaacttgat c 8661 //