ID SPAC4C5 standard; DNA; FUN; 15500 BP. XX AC Z98560; XX SV Z98560.1 XX DT 14-AUG-1997 (Rel. 52, Created) DT 07-MAR-2000 (Rel. 63, Last updated, Version 2) XX DE S.pombe chromosome I cosmid c4C5. XX KW DNA damage tolerance; gtp-binding; haloacid dehalogenase-like hydrolase; KW integral membrane protein; misc_RNA; nitrogen and sulpher metobolism; KW rad31; Ras family; ryh1; snoRNA; snR41 homolog; snR51 homolog; KW snR70 homolog; sulphite reductase; superoxide dismutase; transfer RNA Ala; KW tRNA Ala anticodon TGC. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-15500 RA Pearson D., Churcher C.M., Barrell B.G., Rajandream M.A., Wood V.; RT ; RL Submitted (13-AUG-1997) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: RL barrell@sanger.ac.uk XX DR GOA; O14165; O14165. DR GOA; O14166; O14166. DR GOA; O14167; O14167. DR GOA; P17608; P17608. DR GOA; P79064; P79064. DR SPTREMBL; O14167; O14167. DR SWISS-PROT; O14165; YDX1_SCHPO. DR SWISS-PROT; O14166; YDX3_SCHPO. DR SWISS-PROT; P17608; RYH1_SCHPO. DR SWISS-PROT; P79064; RA31_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid SPAC4C5 is overlapped at the 5' end by cosmid CC SPAC22A12 and at the 3' end by cosmid SPAC10F6. XX FH Key Location/Qualifiers FH FT source 1..15500 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c4C5" FT /map="IL" FT misc_feature 1..121 FT /note="nominal overlap with cosmid SPAC22A12, EM:Z99295 FT S.pombe chromosome 1" FT CDS join(1377..1424,1494..1536,1611..1673,1748..1842, FT 1894..2007,2068..2445) FT /db_xref="GOA:O14165" FT /db_xref="SWISS-PROT:O14165" FT /label=SPAC4C5.01 FT /note="SPAC4C5.01, len:246, SIMILARITY:Schizosaccharomyces FT pombe, O59760, hypothetical 26.5 kd protein., (236 aa), FT fasta scores: opt: 734, E():0, (45.3% identity in 232 aa)" FT /gene="SPAC4C5.01" FT /product="conserved hypothetical haloacid dehalogenase-like FT hydrolase protein" FT /protein_id="CAB11172.1" FT /translation="MAAKHVKYMACLFDMDGLLVDSETIYTKTTNLILDRYGKDPLPIS FT VKAQMMGRPGSAAAKVVIDWSNIPMTPQQFVDEQQVIRAKFWSSLKPMPGAESLINNLS FT NHGIDIGVCTHPYAIIKTAHLKHIFEKFGKNVITGDNPSIAPGRGKPFPDIWLKVLNLI FT NESRKQRGLKALTPSQCIAFEDSIPGVKSAKAAGMHVIWVPDAAIKNLVGDQLNEIVDS FT QCETLPSLSEFDINKYLNINSKQA" FT misc_feature 1398..1994 FT /note="Pfam match to entry PF00702 Hydrolase, haloacid FT dehalogenase-like hydrolase, score 75.90, E-value 8.3e-19" FT misc_feature 1425..1430 FT /note="gtatgt, splice donor sequence" FT misc_feature 1472..1493 FT /note="ttaacaatcactctttttacag, splice branch and acceptor" FT misc_feature 1537..1542 FT /note="gtatgt, splice donor sequence" FT misc_feature 1589..1610 FT /note="ctaatctttattttttttatag, splice branch and acceptor" FT misc_feature 1674..1679 FT /note="gtatgt, splice donor sequence" FT misc_feature 1730..1747 FT /note="ctaacttgcgatacgtag, splice branch and acceptor" FT misc_feature 1843..1848 FT /note="gtacgt, splice donor sequence" FT misc_feature 1879..1893 FT /note="ctaactttctgaaag, splice branch and acceptor" FT misc_feature 2008..2013 FT /note="gtgtgc, splice donor sequence" FT misc_feature 2042..2067 FT /note="ctaataccgcaaattataatatgaag, splice branch and accept FT or" FT CDS join(complement(3746..3868),complement(3543..3702), FT complement(3352..3463),complement(3029..3239)) FT /db_xref="GOA:P17608" FT /db_xref="SWISS-PROT:P17608" FT /label=ryh1 FT /note="SPAC4C5.02c, len:201" FT /gene="ryh1" FT /gene="SPAC4C5.02c" FT /product="gtp-binding protein ryh1" FT /protein_id="CAB11173.1" FT /translation="MSENYSFSLRKFKLVFLGEQSVGKTSLITRFMYDQFDNTYQATIG FT IDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAIIVYDITNHNSFVNTEK FT WIEDVRAERGDDVIIVLVGNKTDLADKRQVTQEEGEKKAKELKIMHMETSAKAGHNVKL FT LFRKIAQMLPGMENVETQSTQMIDVSIQPNENESSCNC" FT misc_feature join(complement(3742..3832),complement(3543..3710), FT complement(3352..3463),complement(3032..3239)) FT /note="Match to PF00071 ras, Ras family Score 230.40" FT misc_feature complement(3240..3254) FT /note="ctaacggtggtttag, splice branch and acceptor" FT misc_feature complement(3346..3351) FT /note="gtatga, splice donor sequence" FT misc_feature complement(3464..3477) FT /note="ctaactacttatag, splice branch and acceptor" FT misc_feature complement(3537..3542) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(3703..3717) FT /note="ctaacagggtccaag, splice branch and acceptor" FT misc_feature complement(3740..3745) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(3794..3817) FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT tRNA complement(4424..4495) FT /note="tRNA Ala anticodon TGC, Cove score 68.49" FT CDS 5827..6735 FT /db_xref="GOA:O14166" FT /db_xref="SWISS-PROT:O14166" FT /label=SPAC4C5.03 FT /note="SPAC4C5.03, len:302aa" FT /gene="SPAC4C5.03" FT /product="hypothetical integral membrane protein" FT /protein_id="CAB11174.1" FT /translation="MEFSTSSFIFDDDTPPKCPLATKASFIFSVYIIIGLIISYLLQIF FT RIVRLGSSQGLSFSYLILGYIGVLNAFSNVIALQISPLTECCQNYYSKKQCFANTTGLI FT QVGSQVLIMGCVLMAFWMFLPRPIHFVAADDENGLPIPEPLSVTKSRKWRKASYGLLGV FT FTWGLFIICLSATVLSSNFAWAAFLGFSASFCAVVQYVPQIIKTIRHQSHGALSIPMMM FT MQTPGGFLIGYLLSRLPGTNWTTYMMYIVSACLQGLLLMLCMFYLHKEKSRRKREELQA FT TLQEEESQRAVRILEAETGPE" FT CDS join(7291..7373,7424..7852,7906..8051,8130..8229, FT 8286..8451) FT /db_xref="GOA:P79064" FT /db_xref="SWISS-PROT:P79064" FT /label=rad31 FT /note="SPAC4C5.04, len:307" FT /gene="rad31" FT /gene="uba4" FT /gene="SPAC4C5.04" FT /product="dna damage tolerance protein rad31; ubiquitin FT activating enzyme" FT /protein_id="CAB11175.1" FT /translation="MGNHNINAEEIALYDRQIRLWGFNAQQALKQSRVLLITASPLANE FT IAKNLVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVEID FT TDTSLISEIDEGKISKFSMVIATQLDYEEFCRINELTRICNASFYATSCFGLYGFAFCD FT LINHNFAIDRVVDNTKVEEDMFIVQKPMKEAFQSILGETLKPRLAKKIPTLYPAMLSLL FT KSKKSDPDSIRQVCIEQKLNEKTVLNGEFLSKFSSNISFQWTPVMSVVGGVVSQDALNS FT ISKKQFPIDNFWIFDAESGLAPIYRL" FT misc_feature 7374..7379 FT /note="gtaagt, splice donor sequence" FT misc_feature 7409..7423 FT /note="ctgacaaaattatag, splice branch and acceptor" FT misc_feature 7428..7835 FT /note="Match to PF00899 ThiF_family, ThiF family Score FT 153.39" FT misc_feature 7853..7858 FT /note="gtaagt, splice donor sequence" FT misc_feature 7893..7905 FT /note="cttacatttttag, splice branch and acceptor" FT misc_feature 8230..8235 FT /note="gtaagt, splice donor sequence" FT misc_feature 8272..8285 FT /note="ctaattttatttag, splice branch and acceptor" FT misc_RNA 9000..9085 FT /note="putative snoRNA Sp-snR41, complements Sp-18S-rRNA FT and modifies Gm1142. Yeast snR41 homolog" FT misc_RNA 9226..9333 FT /note="putative snoRNA Sp-snR70, complements: Sp-18S-rRNA FT and modifies Cm1680. Yeast snR70 homolog. (Todd Lowe)" FT misc_RNA 9478..9561 FT /note="putative snoRNA Sp-snR51b, complements: Sp-25S-rRNA FT and modifies Um2823. Yeast snR51 homolog. (Todd Lowe)" FT CDS complement(9909..14330) FT /db_xref="GOA:O14167" FT /db_xref="SPTREMBL:O14167" FT /label=SPAC4C5.05c FT /note="SPAC4C5.05c, len:1473, SIMILARITY:Saccharomyces FT cerevisiae, YJ9F_YEAST, hypothetical 161.2 kd protein in FT nmd5-hom6 intergenic region., (1442 aa), fasta scores: opt: FT 2177, E():0, (44.5% identity in 1497 aa)" FT /gene="SPAC4C5.05c" FT /gene="SPAC10F6.01c" FT /product="putative sulphite reductase" FT /protein_id="CAB11176.1" FT /translation="MSVKASINNSQEAVSRIAFRCSDALYVVHPNNSILNGALTESLKD FT LKKFETLNVSGKVPHVLPLKSHADPFAHIADAILAEEEVASTQPKQITSVVASADALFF FT ATPHLYKLAHEPLVAHVAIESTEAFDFASVRDTGFVILFSGNRPGDSSEAALEDTLETA FT SLAHRLALKLNTGVLHFYSPVYDTTAALENIETLPSKEDAQHARVAHIPIEEKQEDSEK FT EGNIKEAFVPPKFDQPERDAATSEYLESLSIKPFEYSGSDDATDVLLVFGSAASELAKA FT AVTSSVAVAIVRVLRPWLPSKLQEVLPTSTKRLTVLEPITSLPRKWDPLYLDVLSSFVA FT SGSSIELFAVRYGLSSSEQATEIIKAVRDNLSGALKPSLVCDFTDGVSQVFVPTPPSIE FT EAYHKLLHRVFKSRLNIVNDPASSATKQNIPSRLIISPQFALGSVLEYENQRRAFCDEV FT ATLLKEKNSSVSSESLEVLSNWIVSVDNLESPVDPELVISELKKDSSAPIKSLLDRSEF FT FTNVSHWIIGSDAWAYDLGNSALHQVLCLEKNVNLLIVDTQPYSTREAVRSSSRKKDIG FT LYAMNFGNAYVASTALYSSYTQLISALLEADKFKGPSVVLAYLPYHSADDDAITVLQET FT KKAVDIGYWPLYRWTPALEDGEYSDFKLDSERIRRELKTFLERDNYLTQLTLRVPSLAR FT TLTQSFGAEVRHQQNVDSRNALNKLIEGLSGPPLTILFASDGGTAENVAKRLQNRASAR FT GSKCKIMAMDDFPIEELGNEKNVVVLVSTAGQGEFPQNGREFWEAIKGADLNLSELKYG FT VFGFGDYEYWPRKEDKIYYNRPGKQLDARFVELGAAPLVTLGLGNDQDPDGWETAYNLW FT EPELWKALGLDNVEIDIDEPKPITNEDIKQASNFLRGTIFEGLADESTGALAESDCQLT FT KFHGIYMQDDRDIRDERKKQGLEPAYGFMIRARMPAGVCTPEQWIAMDDISTKWGNHTL FT KITTRQTFQWHGVLKKNLRNTIRNIVKVFLTTLGACGDVARNVTCSSTPNNETIHDQLF FT AVSKQISNELLPTTSSYHEIWIEDPETVEKRKVAGEAVQDVEPLYGPTYLPRKFKVGVA FT APPYNDVDVYTNDVALIAIIENDKVLGFNVGIGGGMGTTHNNKKTYPRLATVVGYVLTD FT KIMEVVKAILIVQRDNGDRENRKHARLKYTVDTLGVSTFVEKVEEVLGYKFEEARDHPQ FT FIKNHDDFEGWHKTEKNKYWRSIFVENGRIENNGILQFKTGLRELAERLYTEKSEAEFR FT LTANQHVILFNVAENELGWINEHMAKYKLDNNAFSGLRLSSAACVALPTCGLAMAESER FT YLPKLITKVEEIVYEAGLQKDSIVMRMTGCPNGCSRPWVAEIACVGKAPNTYNLMLGGG FT FYGQRLNKLYRSSVQEKEILNLLRPLIKRYALEREDGEHFGDWVIRAGIITAVENGGAN FT GAVHEGVSPEAF" FT misc_feature complement(9966..11648) FT /note="Match to PF01077 NIR_SIR, Nitrite and sulphite FT reductase Score 713.97" FT misc_feature 12067..15500 FT /note="nominal overlap with cosmid SPAC10F6, EM:AL009197 FT S.pombe chromosome 1 (NB true overlap with cosmid c10F6 is FT 23900bp)" XX SQ Sequence 15500 BP; 4957 A; 2878 C; 2726 G; 4939 T; 0 other; cggagccgca tgaggagact ttcaaaaatg ttttatcttt gttttctgtc ctcacggcaa 60 tctgcatttg ttatttattg ctgtgttaaa tatccatatt tttcgtcatc cggcaaagat 120 cctccaactc gcctaacagt gaggtacttg tgaccttggt taaaacaaac tttgactgtt 180 tttctacact cggtccaaca atttcacatt atagttgaca cgattctacg acatggctaa 240 cgtgcgtcgc tattgtactt gtaaattttg tctgaccagc tcttcttttc aaaatacatg 300 gccaaaatgg gttccaatag ctaactgatt agtcaccatt ttatgaacag aatccaagtg 360 gctttatcag aaacgcttta aaaaccaaag agagtaatta aaacaaaaaa aattcaacat 420 agcatacttc atgagggaga atggctggtc attgagactt ttccttttcc ctcaacttct 480 gtttatacaa tgttttagta tgagattcct gggcgaatca ttgcctgctt gattcgagaa 540 taatgcgtga gctgttcgct atgttcaatt ctgttataca tcgcaatatg tcaaatgttt 600 tatttctcta ttcactgtac ataaatgata caggataggt tcatcattgc ttatagtcag 660 ctggatatta ttcattgata tataatgaga gcctaacaga accagtatta ttcaatttgg 720 aaatgaacga tctcagtgtc aaaatattca tcaccttcat agaaaaatat cccaaaacaa 780 tcgctaactt acactacctg ggtaccgcga tatagataag taaattacaa ggaaaattca 840 ccacagctta aggcatcatt aaaaccggat tacaatttca tggttaatat aaacgcttaa 900 ttctctttca gtagtgcggt tgaattctca ctaaaacaga ttaacgaact cttcaatatt 960 gctcccaatc atccgacaca cggatttact ctaatctacg attgttgatt acttattgca 1020 gtttgcacag ttttgggatt tagctatttt tcattctgcc taattatcac gccttactgt 1080 atcgacgttt ctttgttgat ctttaacaac tgtccttatt ttttggtttt tcgcggattt 1140 ttttaattag atatacagtt aacacttcat ttgtcggtgt ctttgttttc tttaattgta 1200 tttgtatatt taattgttaa ttcttgtttt ggaagatttt atactttttt attggttata 1260 cagcaatata cttttttgaa acttgatttt ttggatatcc cggaagtttt ttttctgctt 1320 ttttttttct ttggttttgt taggtcgtag aagcttcact atctttttac ctagaaatgg 1380 ctgctaaaca cgtaaaatac atggcttgtc tattcgatat ggatgtatgt ttatttttca 1440 caccacatta cctcttatga aagcttttgc attaacaatc actcttttta cagggtctac 1500 ttgtcgacag tgaaactatt tatacaaaga cgaccagtat gtaaataata tatctgtgct 1560 tatatttagc acaaaactac aatgttttct aatctttatt ttttttatag atcttatatt 1620 ggacaggtat ggcaaggatc ctcttcctat ttctgttaag gctcaaatga tgggtatgtt 1680 atataaagct agcgaagaga tattttaaaa tgctttcaaa attgcaggtc taacttgcga 1740 tacgtaggga gaccgggttc cgcagctgcc aaggttgtta tagattggtc caacattcct 1800 atgactcctc aacaattcgt tgatgaacaa caggttattc gggtacgtac ttatacgcta 1860 catttatttc catttcaact aactttctga aaggcaaagt tttggtcgtc tttgaagcct 1920 atgcctgggg ctgaatcttt gattaataac ctctccaatc atggaattga tatcggggta 1980 tgtactcatc cctacgctat aataaaagtg tgctaacagc atcaccagct tgctacttcc 2040 tctaataccg caaattataa tatgaagaca gcccatctta aacatatttt tgagaaattt 2100 ggtaaaaatg ttattacagg tgataatcct agtattgcac ctggtcgtgg caaacctttt 2160 ccagacattt ggctgaaggt gttgaattta ataaatgaaa gtaggaagca gcgaggtttg 2220 aaggcactta caccaagcca atgcattgcc tttgaagaca gtattcccgg tgtcaaaagt 2280 gcaaaggctg caggaatgca tgtcatttgg gttccagatg ctgccatcaa aaacttggta 2340 ggggatcagc tgaatgagat tgttgattct caatgtgaga cgttaccgag tctttcagag 2400 tttgatataa acaaatatct taacataaat tcaaagcagg cctagaggtt atatccgcta 2460 actttttttt ggttcattca attttttttg gcaaaaaacc tcatatcttt taataaaatt 2520 aaaaaattga agtataaata aagttctact gtttcaatga aaaccttgaa ctgactcaca 2580 tagcataaat aacttatgag taaagggaaa aagcgaaatc taaaaaaaaa aaaataataa 2640 catttctctg aattccaaaa tcatacattg taattttttt ccttctttgc aaatgccctt 2700 gaaaagtcaa gaagcaccgc agggattaaa cgaaagagac ttattcagaa attcaacttc 2760 tcacttttgt aaagcatata tcatagagac aggattaaaa taaaaaagga ctaaaacaag 2820 taaaaaaaga aggaaaagga aacaggtata aaaggaatcg cgattcttta aaggtgcaaa 2880 agatttaggg gctataagaa gatatatata tactcacagt atactttttg agggtcaaat 2940 gacattaaaa accgctgatt gcggcctgaa gcgaaaaaaa ataattcacg aaacagttag 3000 agtacactga catgttcaat gagatacact agcaattgca gctgctttca ttctcgttag 3060 gctgaatgct aacgtcaatc atttgagtgc tttgtgtttc cacattctcc atgcctggta 3120 acatttgtgc aattttccta aacaacagtt ttacattgtg acccgcttta gcactagttt 3180 ccatgtgcat tatttttaat tcttttgcct ttttttctcc ttcttcttga gtcacttgcc 3240 taaaccaccg ttagcataag aaccatcaaa aaccaaaata tagctttgga attatgaatg 3300 aagagatgtc gcattgtaaa tgctgaatct ctgttagctg gagtgtcata ccttttatca 3360 gccaagtccg ttttattacc aacgagaaca attataacgt cgtctccccg ctcagcacga 3420 acatcttcaa tccatttctc agtatttaca aaagagttat ggtctataag tagttagcag 3480 atattcgaaa ataaagtctg agtaaaaata tatggcaaaa ttactgtgcc actattactt 3540 actagtgata tcgtaaacaa taatagcaac agaagaatcc ctaatatacg aaggaattaa 3600 agaacggaaa cgttcttgac cagcagtatc ccatagttga agacgaaccg ttcgatcttc 3660 caaatacata gtcttgctta gaaagtctat accaatagta gccttggacc ctgttagtaa 3720 agaagtttta attatagtta cttacttgat aagtattatc aaactgatcg tacataaatc 3780 gtgtaattaa cgatgttttg ccaactgact gctcaccgag aaaaaccaac ttaaatttac 3840 gcaaagaaaa cgagtaattt tctgacatta tgacaaaaag ttttgacttc taaatataca 3900 cagacaaaag taatttagat acagacggaa ttgcttattg atgatcaaaa agcaccgtac 3960 ttaatagtgg tagtagtgag taaagactat cgtgctacag tgagatcccg ccgcatatcc 4020 ttaatatact ttcgcttgtt ttacttttca ttcgctttta cgaagtttaa tctcggtaaa 4080 gtaattctat aacaaacaac tttttcgctg aaaaagaaat catgaactct caccgttctt 4140 aaattactaa gcattcctaa agatgggttc tttttctttg agttgttgat agtgataggt 4200 ttttgggtgt taaaaaacat acagctgtaa caacagtttg tttactactc atagttttat 4260 acacaacatt tcaaggaatg acaactggga taaaaacgcg ccacatcgca tgggaaggag 4320 gagtcatcga gatttgatta tcattaaact atataataca agaaatctct cgattggaat 4380 ggcctaattt ttgctgagag tagctaagtt tacaaaaaaa tattggacac gctggggatt 4440 gaacccagga cgactcgcat gcaaagcgag catgctacca ctgcatcaca tgcccaaaca 4500 ttgaaggctt cagcatatta gtaaagtata aggtaatttt tttgtttagt agctgatgaa 4560 agaaggaaaa tggaaagtag ttaaaccata gaacaagctt gaaattcaac ataatggctt 4620 tattccattt gaaattttat tagaatgttt cataatctac gcatcctgtc tgctaccaag 4680 ttcaagattg gcagactgca ccaaactgtt cgaaaaatgt atgtttgtta acttgaataa 4740 aactagctgt taaacgagtc agatttaagc aaaatataag ctttacagga aaatatactc 4800 gagttaattt agcaaactcg gtcatttcat taatgataca attagctacg gaacagccaa 4860 gtcaaatttt atacttaatg tcaacaataa caggttagtt agtgggtttt aatttaataa 4920 ttttcgaaat caataaaaga gtaaatagta tggcttttca agcttagatt cttttaaaaa 4980 taaatttttt ttttatttgt atccttattg caatatgacg aaaagttcaa gaaagataag 5040 agaattaaaa actaagttat ttcataatta ttagctgctg tttggaataa aaaatctcgg 5100 aatttaatga taagatgaaa aagaatttaa aaagtaaata tgtagttata tatcctataa 5160 aaaatttggt tcaaacgatt aatttagatt atttagaatc tttaaaaaga taatggggtt 5220 aaaaacaaaa gaacttgatt ttagattcaa tttagaaatt tttttgtaaa gtgtcttgat 5280 gtatttcaat ttttagatgt gtagcgccat ttcatttcac tcatgttttt aaaagagaaa 5340 aaaatttacc tagtttatta agatatacaa ttcattcagt tccttttatc ttcttctata 5400 attcattgtt tatttactta tttattttct ccaatgtcaa atttatagca ctttaaatat 5460 cgcaaaattt caatattata cgttacagct cacaactttg tgtctaagcc ctccgactaa 5520 tgtagcttac actgcctctc ctttccccat tttcgacaaa attaaatgaa aaatccaatt 5580 ttttggcgat tcttcctttt acttatttat taaacaccaa ttatttcaaa aggaaatttt 5640 aaattgtctt tccttagtga cgcttgttca ataagcccca aagttagcaa cctttacttc 5700 atcgtaccat tattggcagc ccaggcatcg tttatttata tatttttata caaagcatta 5760 gaaattaact tcgtattgca tttctttatt acgattttat tataggagac gaagtacgta 5820 tgaacgatgg agtttagcac gagcagtttc atttttgatg atgatacacc accgaagtgc 5880 cctttggcaa caaaagctag ctttatcttt tcagtgtata ttatcatagg cctcatcatt 5940 tcttacctgt tacaaatctt tcgcattgtt cgtctcggaa gttcgcaagg cttaagtttt 6000 tcttacctta ttttgggtta cataggtgtt cttaatgcgt tctcaaatgt cattgctcta 6060 caaattagcc ctttaactga atgctgtcaa aattactatt ccaaaaagca atgctttgct 6120 aacacaaccg gtttaattca agtgggttct caggtactta ttatgggttg tgttcttatg 6180 gctttctgga tgtttctccc tcgacctatt cactttgttg cagcagatga cgaaaatggt 6240 ttaccaatcc ctgagccttt aagtgtaacg aaaagtcgta aatggcgaaa agcatcttat 6300 gggctacttg gtgtatttac ttggggattg tttatcatct gtctatccgc aaccgtcctt 6360 agttcgaact ttgcttgggc agcattcctt ggatttagcg cttcattctg cgcggtcgtt 6420 cagtatgttc ctcaaatcat aaaaactata cgccatcaaa gccatggtgc ccttagtatc 6480 ccaatgatga tgatgcaaac tccgggtgga tttttgatcg gatatttact atccaggttg 6540 cccggtacta attggactac gtacatgatg tatattgtca gcgcatgcct tcaaggattg 6600 ttattaatgc tttgcatgtt ttatttgcac aaagaaaaaa gtagaagaaa gagggaggaa 6660 ttgcaggcca ctttacagga agaagaaagt cagcgagctg tacgaatttt ggaagccgaa 6720 actggtcccg aataataaca ttttctttat gttttcacgt cccttatcta tttgtttacc 6780 gaaaaaattc ctaatatttg tttcattcag ttagtaaggt ttactcttca aaaatttttt 6840 ccgcacatta gaattggatt gcgtgatgtt atacgttttg cttttaaatc catgaaccac 6900 tggtatttat tttatttttt cccacttgat gaatttcagc actcttagtc agattctagt 6960 attccctttc ccacattgct atagatttgc ttatttgtta cttaatctaa tctgtttatt 7020 ttatgtagaa taacattgaa aacgctgttt cgcttctcaa ttaccaagaa tatggtgtga 7080 attaatgcct ctaccaaaca tccttgactt ggccataagt ctctatataa tgtatcagta 7140 atgaactcgt ttgttattta atttccatat taaacactgt tagacgatta gctaaatctg 7200 ccttttcgat atttactacc ttaccccaac ttatatctga ttcacgatag acatagctgt 7260 attcagtcca ctaaccccta agaagaggac atggggaatc acaatattaa tgctgaagaa 7320 atagctttat acgatagaca gattaggctc tggggcttta atgctcaaca agcgtaagtt 7380 ggctgaattt acgcatgata gctgggttct gacaaaatta tagactaaag caatcccgcg 7440 tcctattaat aactgcttct ccattagcga atgaaatagc gaaaaacctg gtattatctg 7500 gaataggaaa actttgtgta ttagattcaa tgactgttta tgaaaaggat gtcgaggagc 7560 aattttttat agaagcttca gacattggcc agctccgcgc taatgtcttc aagaaaaagt 7620 tacatgaatt aaacccttta gtagaaattg atacagatac gtctctgata tctgaaattg 7680 acgaaggaaa aatctcaaag ttcagcatgg ttattgcgac gcaacttgat tatgaagagt 7740 tctgccgaat aaacgaatta acccgaattt gtaatgccag tttctacgca actagctgtt 7800 ttggtcttta tgggttcgct ttttgtgatt tgattaacca caattttgca atgtaagtct 7860 tgtctaagtg atcttcaatt tcaacacctt tgcttacatt tttagtgatc gagtcgttga 7920 taatactaaa gtagaagagg atatgtttat tgttcaaaaa cccatgaagg aagcctttca 7980 aagcatattg ggcgaaacac taaaacccag gttagcaaaa aagattccta cattatatcc 8040 agcgatgtta tgtaagtcat taactgagtt caataaggtt aaatatgatg aagttggtaa 8100 atttttacag ttgctaattg ttaatttagc tttattaaaa agtaaaaaat cagatcctga 8160 ctcgattcga caagtttgca tcgagcaaaa gctgaacgag aaaacagtac taaatggaga 8220 atttttatcg taagtaaacg gaattacttt gactacttga tcactttttt actaatttta 8280 tttagaaaat tttcttccaa tatcagcttt caatggactc cggttatgtc cgttgttggc 8340 ggggttgttt cacaagatgc attgaattca attagcaaga agcagttccc cattgataat 8400 ttctggattt ttgatgccga atcaggcctt gctccaattt atcgtttgta acaccaccta 8460 tgcccagata ttctataccg caagggtatt ctgttattta gcatcattct atgaaaaaaa 8520 tttggagaat ttgtcttttg cgaaattaaa aaaaataaac cgcccttatc atgttattaa 8580 ttgatacaat tatttacaat agtattgcaa ataaaattaa gtcttatatt cagcaatatc 8640 acttacttta tcctatattt attatatcta taacagcttt ttatcctaaa attgtcaaac 8700 gcttccttta cttcgaatga aaagactaag atactcgact gccattaaaa aaataatagt 8760 gagacttaag cgattttatg gcagaaatgt tgaattcgtt aaagacagca gtcacaattt 8820 tgtatactca actcttaaat tgcaacctca gcttccttgt atatatggtt tgtaattatg 8880 gttacttgtt aataacaatc aaagttattt aatttaaaga ttgctaatgt tcttaaaata 8940 gcttatacag gtaagcataa tttaaaaaca gaagttcctg tagctttact actaacaatc 9000 attgtgatga taaaacgacg aaaacagctg aattcctcat gttgaattcg ttatattatt 9060 ttttaagttt cagccttgtc tgatcatgat tttttttact tcttttttct ttattttttt 9120 gttatttttt ctgacgtttt tagagttgaa tcctaatttt ttaaaaagtt gtattttaat 9180 atatttccca taaggtaagt taaaaatttt aggttgcttt tcaagtttaa ggatgatacc 9240 taagacgcaa cgggcggact aaatgggtta ctacaatgct tcaactgtac aatgaagttg 9300 taactcacat gttgttatcg tgtcttactg aaatacttga ttagcattaa attttgtctt 9360 tttttttttt gtttgtatct aaagtattaa ctaagatttt agattttaca tagttactat 9420 tgtgctacat ttttggttca gtaaatctca aatagaccgt tttaatagat agcaacttgc 9480 catgatgagc cattttatat tttgtattca cactgtctga atttcttgaa gatatactga 9540 aactctgagc aaagcctgat tattcattcg tttcttacat ttggtagtca acaatattgt 9600 tatattcctt ctcatagtta atacgtctat ttaaatccaa gtatgctact aagaaaatcc 9660 tggatatagt aacataagat caagtcgata ccataattta taatttgata caaagaaata 9720 tgatttaaag tactttgaat ctaaatataa gaagttgata acatatacaa tttttataga 9780 tatcgtatga caaatcacta tgttaaccta ttaatctaaa accaacaatc attgcgatac 9840 taaaacagac tcattttgac aattataata gtcaacatct aaccaccaac cttgactaaa 9900 atagacgtct aaaacgcttc aggtgatacc ccttcatgaa cagcaccatt agctcctcca 9960 ttttcaacag ctgtaatgat acctgcacga ataacccaat caccaaagtg ttcaccatct 10020 tcacgctcca aggcgtaacg cttgataagc ggacgcaaca aattgaggat ttctttttct 10080 tgaacggaag agcgatatag cttgtttaat ctctgaccgt aaaatcctcc accaagcatt 10140 agattgtaag tatttggtgc cttaccgaca cacgcaattt cagcaaccca tggacgagag 10200 catccatttg gacaaccagt catacgcata acaatactat ctttctgaag accagcttcg 10260 taaacaattt cttctacttt cgtaatcaac ttgggaagat aacgttcact ttcagccatg 10320 gctaaaccac aagtagggag agcaacgcag gctgcagaag aaagacgtaa gccagagaat 10380 gcattattat caagtttata tttggccata tgctcattaa tccaaccgag ctcattttca 10440 gcaacattaa ataagatgac atgctggttg gcagtcaaac ggaattcagc ctcagatttt 10500 tcagtatata gacgttctgc gagttcacgc aaaccggtct taaactgtaa aataccattg 10560 ttctcaatac gaccgttttc aacaaatata gaacgccagt atttgttttt ctctgtcttg 10620 tgccagcctt cgaagtcatc gtggttttta atgaattggg gatgatcacg tgcctcttca 10680 aatttgtaac ccaaaacctc ctcgaccttc tcgacgaagg tagaaacacc aagagtatca 10740 acggtatact taagacgagc gtgcttacga ttttcacgat caccgttatc acgctggaca 10800 atcaaaatag ctttaaccac ctccataatc ttatcagtta gaacatagcc aacaactgta 10860 gccaaacgag gatatgtttt cttgttgtta tgggttgtgc ccataccacc accaatacca 10920 acattgaaac ctaggacttt gtcattttca atgatggcga ttaaagcaac atcattggta 10980 taaacatcga catcattgta agggggagca gccacaccaa ccttgaattt tctgggcaga 11040 tatgtaggac catataaagg ctcgacatcc tgaacggcct caccggcaac ctttctcttt 11100 tcgacagttt cggggtcctc gatccaaatt tcatgataag aggatgtagt aggaagcaat 11160 tcattactga tttgcttaga tacagcaaac agttgatcgt gaattgtttc attgttggga 11220 gtcgacgaac aagtgacatt acgagcaaca tcaccacaag caccaagagt agtaaggaaa 11280 actttaacga tgttacgaat ggtattacgc aaattcttct tcaaaacacc atgccattgg 11340 aacgtttgac gagtcgtaat cttaagggtg tgattacccc atttcgtaga aatatcatcc 11400 atagcaatcc attgttctgg tgtacagaca ccagcaggca ttctggcgcg aatcatgaaa 11460 ccataagcag gttccaaacc ttgtttttta cgctcatcac gaatgtcacg atcatcttgc 11520 atataaatac catggaattt ggtaagttga cagtcacttt cggccaaagc accagtactt 11580 tcatcggcca atccttcaaa aatggtacca cgcaaaaagt tcgaagcttg tttaatgtct 11640 tcattagtga taggcttagg ttcatcaata tcaatttcga cattgtccaa tccaagagct 11700 ttccaaagct caggctccca aaggttatat gctgtttccc atccatctgg atcctggtca 11760 ttaccaagtc ctaaggttac caatggagca gcacccaatt caacaaagcg agcatctaat 11820 tgcttacctg ggcggttata ataaatttta tcttccttac gaggccaata ttcataatcg 11880 ccaaaaccaa atacaccata cttcaattcg ctcaagttaa gatctgcacc tttgattgct 11940 tcccaaaact cacgtccatt ttgaggaaac tcaccttgac cagctgtact aaccaagact 12000 acgacattct tctcattgcc taactcctcg atagggaaat catccatggc cataatctta 12060 cacttggatc ctcttgcaga agcccggttt tgaagtctct tcgcaacgtt ttcagctgta 12120 cctccgtctg aagcgaaaag aatggtaaga ggaggaccag acaaaccttc aattaatttg 12180 ttgagagcat tgcgtgagtc aacgttttgt tgatgacgga cttccgcacc aaatgattga 12240 gtaagagtac gagctaaact tggcacacga agcgttagtt gagtcaagta gttatccctt 12300 tccaaaaagg tcttgagctc tcggcgaata cgttcggaat caagtttgaa atctgaatat 12360 tctccgtcct ctaaagcagg agtccatcta tacaaaggcc aataaccgat gtcaacggct 12420 ttcttggttt cctgtaacac agtaatggca tcatcgtcag cactgtgata gggaaggtat 12480 gccaaaacaa ccgaggggcc cttaaattta tctgcttcta aaagtgccga gattagttgg 12540 gtgtatgaac tatacaacgc agtagaggcg acatatgcat tgccaaagtt catggcatac 12600 aatccaatat ccttctttct ggaagaggat cggacggctt cacgagtaga gtaaggctgg 12660 gtgtcaacaa tcaataaatt gacgttcttt tccaagcaaa gtacttgatg aagagcagaa 12720 ttacccaagt catatgccca agcatcggaa ccgataatcc aatgtgaaac gttggtaaaa 12780 aattcagatc gatctaaaag tgatttgata ggagcagatg aatccttctt aagttcagag 12840 atgaccaact cagggtcaac aggagactcc aggttatcaa cggaaacaat ccaattcgaa 12900 agaacttcta atgattcaga agagacagaa gaattctttt ctttcaataa agtagcaacc 12960 tcatcacaga aagcgcgacg ctgattttca tactctaaaa ccgaacccaa agcaaactga 13020 ggagatatga tgagacgaga tggaatgttt tgtttcgtag ctgatgaagc gggatcgtta 13080 acaatgttaa gacgagactt aaagactcta tgaagaagtt tatgataagc ttcctcaata 13140 gacggaggtg taggaacaaa cacttgagaa acaccatcgg taaaatcgca aacgagagat 13200 ggctttaaag cgcctgaaag attatcacga acagctttaa taatctctgt ggcttgctct 13260 gaagaactca atccgtatct tacggcaaac agctcaattg aagatccgga agcgacgaaa 13320 gaagataaaa cgtcaaggta aagcggatcc catttacgag gaagactggt aatgggttcc 13380 aaaacagtca agcgcttagt agaagtagga agcacttctt gcaatttaga gggcaaccaa 13440 gggcgaagga ctcttacaat agcaactgcc acagaggaag taacggcagc cttggctaat 13500 tcagaagcag cgcttccgaa aactaacaag acatcagtag cgtcatcgga accagagtat 13560 tcaaaaggct tgatagacaa tgattcaagg tattcagaag tagcggcatc acgttctggc 13620 tgatcaaact tgggagggac aaaagcttcc ttgatattgc cttctttttc agaatcctcc 13680 tgtttctctt caataggaat gtgggcaact ctagcgtgtt gagcatcctc tttggaaggt 13740 agtgtctcga tgttctccaa cgcagcggtc gtgtcgtaaa ctggagaata gaaatgtaaa 13800 acgcctgtgt tcaatttcaa tgctaagcga tgagccaaag aagctgtctc caaggtatct 13860 tccaaagcag cctccgaaga atcaccaggg cgattgcccg aaaagagaat aacgaaaccg 13920 gtgtcacgca cagaagcaaa atcaaatgcc tctgtagact ctatggcaac gtgcgctaca 13980 agaggctcat gagccaactt ataaagatga ggagtagcga aaaagagtgc atcagcagaa 14040 gcaactacag atgtgatttg tttaggttga gtagaagcca cttcttcttc agccaaaata 14100 gcatccgcaa tgtgagcaaa gggatcagcg tgagatttta aaggaagaac gtgtggaacc 14160 ttgccggaaa catttaacgt ttcaaatttt tttaaatctt ttaaggattc tgttaaagca 14220 ccatttaaaa tcgaattgtt aggatgaacc acgtacaacg catctgagca acggaaagcg 14280 atgcgtgaga cagcctcctg agaattattg atactagcct ttacactcat gactatttct 14340 tatgacaagt atttgaatat gaattctctt ccttgtcgta ctttttttcc ttcaaaaaaa 14400 ccaaacaaaa caaatagaaa gaagagtaaa aagcccgacc ctatataata tgcaataaaa 14460 aatataccaa aatcttaaaa caccaaagcc ttgctactac actcaaagag ttgtcaaaac 14520 ttatttttaa aaacgacgaa ccgaacgaaa gcgtttaata taacactcgg tttaagttag 14580 actcaaaagt agaaaacaaa gctgatgaga gatggaagag aaaaaaaaaa tggttaaact 14640 taattaacgt tctcttcaga ttatccctca acgactgcaa actttcaact attttctaag 14700 aatgaaaatt gtgcgacctc cttgaaactc ttcgtaggaa gaagtcaata tgtttaaaaa 14760 tctttttttt ttcacgtgta ttcagtgcga aaatcgatac tgaaacagca atttccaaaa 14820 ttccttgaaa aattcgttta aaaggttggt ggtggttttt tgaaaacaac tccgtgccta 14880 ctcaatgtat atacagtcct acttcaagcg tatcggtgtt catacaattg cgtgataaga 14940 cgcaatactg caatacagac actgaaggaa gctacctgag gtaacatatt tttgcaggtg 15000 taccgtccaa tgaggtgggt cgcaatctag gatccaagtc tcaggtgttg gcatctaagc 15060 agtttgtgaa tcttacgatg agttccccag cttttcagga gaagcaaatt tctctcaaat 15120 ctccttaaca cgattacttc tctggattat acaaccttat tattcccatc gcctgaaaat 15180 tacggatcat tttgtaacgg ggcacgcaat actgtaagcc aacagcatta ttacgtggta 15240 ttgcgtggaa tggcattaat tttaacgcgc gctttgatta aggtggtaag cacaatcaca 15300 gagatctaag gcaagcgaca gatggctcaa tattgtctcg gctaggggtt agagttattt 15360 cgagcgagaa tgagagtaat ttacacgttg tatgctgcca tatcataaga gtgtttgggt 15420 ctagccggtg atcagttttt tttttttcct ttccacagta gagagggatt tttggcgtca 15480 tctcgtacta tgaaatactc 15500 //