ID SPAC458 standard; DNA; FUN; 13961 BP. XX AC AL358272; XX SV AL358272.1 XX DT 06-JUN-2000 (Rel. 64, Created) DT 06-JUN-2000 (Rel. 64, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c458. XX KW methyltransferase; nuclear segregation; phosphatidylinositol 3-kinase; KW transcription initiation factor TFIIE alpha subunit; vps34. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-13961 RA Harris D., Lyne M., Rajandream M.A., Barrell B.G.; RT ; RL Submitted (05-JUN-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; P50520; P50520. DR GOA; Q9P3W1; Q9P3W1. DR GOA; Q9P3W7; Q9P3W7. DR SPTREMBL; Q9P3W2; Q9P3W2. DR SPTREMBL; Q9P3W4; Q9P3W4. DR SPTREMBL; Q9P3W5; Q9P3W5. DR SPTREMBL; Q9P3W6; Q9P3W6. DR SPTREMBL; Q9P3W7; Q9P3W7. DR SWISS-PROT; P50520; VP34_SCHPO. DR SWISS-PROT; Q9P3W1; T2EA_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c458 is overlapped at the 3' end by plasmid pYUG7, CC EMBL entry SPAPYUG7, accession number AL136521. XX FH Key Location/Qualifiers FH FT source 1..13961 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c458" FT /map="IR" FT CDS 1..942 FT /codon_start=1 FT /db_xref="GOA:Q9P3W7" FT /db_xref="SPTREMBL:Q9P3W7" FT /label=SPAC458.01 FT /note="SPAC458.01, len:313, SIMILARITY:Drosophila FT melanogaster, Q9W4M9, cg6133 protein., (746 aa), fasta FT scores: opt: 337, E():2.2e-14, (28.2% identity in 284 aa)" FT /partial FT /gene="SPAC458.01" FT /product="putative methyltransferase; similar to yeast FT ncl1" FT /protein_id="CAB93843.1" FT /translation="DLNIYPSFDTLPKELYEKMPPTLWPLPKKELAELNIQNCLRIYPH FT FQNTGGFFVAVLEKYENLTSSMKTAVDDNKVFLREQKLPSEQASKKRKQDTQETSSDSK FT LAEVKPKGKNGGNRFHELDPFVYIKEDDQALEKIYKKFGIDEAIIKKNQFFVRNVNGVP FT TKAIYISNDLFRNVIENNRNRVKFVHGGLKIFVRQDFGSLSREIAEKNGTCVFRVQSDG FT ANLASHFIAESCLFHTTLSDLFILLDHEAVTIDDFPEDSLFRKEYNHLDLGSTLLHVDL FT AKEESVIKKQVYIPLWKSVRICNVLLSNSEKR" FT CDS join(complement(2765..2860),complement(2471..2644), FT complement(1237..2373)) FT /db_xref="SPTREMBL:Q9P3W6" FT /label=SPAC458.02c FT /note="SPAC458.02c, len:468, predicted to contain FT coiled-coil regions, SIMILARITY:Saccharomyces cerevisiae, FT BFR1_YEAST, nuclear segregation protein bfr1., (470 aa), FT fasta scores: opt: 574, E():1.5e-23, (26.3% identity in 463 FT aa)" FT /gene="SPAC458.02c" FT /product="similar to yeast nuclear segregation protein Bfr1 FT involved in Mitosis and Vesicular transport" FT /protein_id="CAB93844.1" FT /translation="MSSHKPVRPNEALHKEALEELDAKINEAKKRFNEHKEKLGAIRGG FT GSLQEKNAELRAELDNIRNAQAAIRSSKQTLINKVKAQDELLKKKVKELTAMKKTVPFK FT SEVELDKHVKQLQAAVDSGTLKIVDEKKYLREISQCNRTRKSFVELNALQTSIDTIRNE FT LNELRDQLNDSESKKLSDKFVEIRSELDEVRKQQDGYYKDQRKLIAERDDEKTALDDLY FT NQRRALQREYDTQLRAFRTYEREQRAKRQEQFRLERENREKEKRRIAAQRKLEEASIPA FT FTEEILACENLLKVFHVPVESSTTNAVSTGNTSSKILKPRTLTPRTVDPIPEGTIIKKE FT SSDDAMFSGLKKSKPKKSNKSNNNQADSDRLNLSFGTIKEFDFVGVPAPFTKSQVDSAV FT EQLKSRIAHFKEQQDSVTKQRIEKAKQEIEKLEAKYNSKEEKTLTEADMVISSEETVTV FT TNDLEVEATA" FT misc_feature complement(2374..2388) FT /note="ctgacatacttttag, splice branch and acceptor" FT misc_feature complement(2465..2470) FT /note="gtatgt, splice donor sequence" FT misc_feature complement(2645..2656) FT /note="ctaatcaactag, splice branch and acceptor" FT misc_feature complement(2759..2764) FT /note="gtaagt, splice donor sequence" FT CDS 3907..6513 FT /db_xref="SPTREMBL:Q9P3W5" FT /label=SPAC458.03 FT /note="SPAC458.03, len:867, SIMILARITY:Saccharomyces FT cerevisiae, Q05543, chromosome iv cosmid 9819., (409 aa), FT fasta scores: opt: 126, E():0.75, (23.8% identity in 244 FT aa)" FT /gene="SPAC458.03" FT /product="hypothetical Leucine-rich protein" FT /protein_id="CAB93845.1" FT /translation="MKSFASELSKPIQKPRLKEILKELQHIGVPSPCNLRLYHELISVI FT VPTFWNGKINSEIDFLLAKCFSTLLGLKLLCVRLDNFVQEANLQNIHLVTVYLELLEKA FT FECIDLDDNCKLIWESTVLSDHQKQSLWTEFIFFLGNFKITNSMSAAMLTFKVYDDSKR FT LKASSMADYIGVLSSKLACIISKPAVKTPEIYSKLFHYLLHSSNLKAFINPLIPLTQKF FT CVQLQKLFADLTVSDQMLFLNQLLLEHNTKYPTNFSYSTARDDRITGSLATLLRLNFSS FT THFLRLIEFYWGVPTNLIIKRVEVVCSSISYKESDKKELEKTVDSLFNIWSNAQFLRSY FT TLLAQESLTIYLLLFIPKLEAKYLNNLSKSLMFSNAISCRLDSLDDDIRMHGMIMAEVI FT STYSGVSLSNPLAFDVPIMKTTKAKVLKSLSSLKDEFLPIEILNDESVIAETSIEKEET FT NVTHLEKPVISKNDDLIRGDDLEPYDFPDIDSEDTDDDPTVSRSKTHSPVYVQDLCKML FT KDTESFEKQRVALENASKLIKRKSAFGTELRDHADELLQTLISLQNRFDLMNFDEMQMT FT AIVELLLTCLDICGPVICTNLFVSDYSMRQKILILSCISLAASKFNDDDNERLFPSQLL FT PGNLHDQFYSPTIEKISDELERKLVFPVMSECKDYAEGPKFLQTRYISKQSEIESKKPL FT PKANRLSLKVDKSLFLLLTSGYMLASRKTAFIEPLFLVYYLKTLGILFFETFTSKIEGA FT NRMLREYVDVLNEVCRLRSDTPEVNEGLLFSFLAILEISSGRTLANEFGKHLLFFEAYT FT KFLFESPDQGEKVKSLSAAVLILFENKLSEFRTLALEKLLDDSAPLVPERFGLAGL" FT CDS join(complement(7375..7759),complement(7234..7328), FT complement(6650..7192)) FT /db_xref="SPTREMBL:Q9P3W4" FT /label=SPAC458.04c FT /note="SPAC458.04c, len:340, SIMILARITY:Saccharomyces FT cerevisiae, Q03789, pam1p, (659 aa), fasta scores: opt: FT 109, E():8.6, (21.9% identity in 302 aa)" FT /gene="SPAC458.04c" FT /product="hypothetical protein" FT /protein_id="CAB93846.1" FT /translation="MNEHGCFSGGSYSELSNDCETVNFRGIAYKCFFTKDQVKGTRLVR FT LTNYLMLLVNFWQISHLTGGLFNFLVSQALNEHGLDLLWIIMVVAQTLSALVSFPEKLL FT NILRNMKTILINTEETVRNRFEESQNKKLVALLDKNSTTLDISSGILLNFTVVLKQLKH FT ALGLHTSVDNSQEFILQFLRTTLLSVPTSSIVSISADPTSWNNLNVLMKYNFMFQKFKP FT RDFHAQTIQSETMFIPPCWDTISKIQSVNHEFNIALFKQTAKNFYDTLDISLLLGLFDK FT SISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKSSLWKDLIQ FT " FT misc_feature complement(7193..7203) FT /note="ctaatttgtag, splice branch and acceptor" FT misc_feature complement(7228..7233) FT /note="gtaaga, splice donor sequence" FT misc_feature complement(7329..7342) FT /note="ctgatctgctttag, splice branch and acceptor" FT misc_feature complement(7369..7374) FT /note="gtaaga, splice donor sequence" FT misc_feature 9282..9644 FT /note="Match to PF00792 PI3K_C2, C2 domain Score 62.21" FT CDS 9282..11687 FT /db_xref="GOA:P50520" FT /db_xref="SWISS-PROT:P50520" FT /label=vps34 FT /note="SPAC458.05, len:801" FT /gene="vps34" FT /gene="SPAC458.05" FT /product="phosphatidylinositol 3-kinase vps34" FT /protein_id="CAB93847.1" FT /translation="MDRLVFSYCPSSKVTARFLVKFCFIEYQDSQEPCICTIQLFSGNE FT SGSLMQKCFVSKIPNKSLLPTELSKISTHEWLDFGVTVSELSLNAKFVVSAWKPSFNDE FT EVYEFVGCTTYRLFDENNLLRQGLQKIPLQTSKEIKKYSPTSLELEQVKEINRLDGLLL FT KLQLGDVPSVNWLDDISFGKIKDFRSKHMSLVTIPILYLDFLQFSFPVVFQRSYYPKSE FT NRVYYSSFDLELNLDSPAELKHRRLVRSQRNGPLDKDLKPNSKIRKELESILSYPPSEE FT LSLEEKDLIWKFRFYLTRNKKAMTKFLKSVVWTDSSEVNQALSLLDSWTEIDIDDALEL FT LSPSFVHPKVRAYAVSRLETASNEELLLYLLQLVQALRYDNPISSDERFQPSPLALFLV FT NRAISSPSIGNDLYWYLVVEIEDEPVSKLFSSVMFLFQKELSKSVEGRLIRETLSAQAK FT FVEKLLRISKSVQSFRGTRLKKIEYLKVLLEDHKYHLLDFHALPLPLDPSVNIVGIIPD FT ACTVFKSTMQPLRLLFKCQDGSKYPIIFKNGDDLRQDQLVIQILTLMDKLLKKEKLDLH FT LKPYRILATGPTHGAVQFVPSKTLATILAEYHGSVLAYLRENNPDDGLNSANYGIDPVA FT MDNYVRSCAGYCVITYLLGVGDRHLDNLLITKDGHFFHADFGYILGRDPKLFSPAMKLS FT KEMVEGMGGYNSPFYQQFKSYCYTTFTALRKSSNLILNLFSLMVDANIPDIKFDKEKVV FT YKVKERFCLQMSESDAIKYFEQLINDSVSALFPQIIDRMHNLAQYMRS" FT misc_feature 10050..10622 FT /note="Match to PF00613 PI3Ka, Phosphoinositide 3-kinase FT family, accessory domain (PIK domain) Score 365.24" FT misc_feature 10788..11675 FT /note="Match to PF00454 PI3_PI4_kinase, FT Phosphatidylinositol 3- and 4-kinases Score 377.38" FT CDS join(11914..12028,12080..12180,12229..12317,12374..12465, FT 12515..13212) FT /db_xref="SPTREMBL:Q9P3W2" FT /label=SPAC458.06 FT /note="SPAC458.06, len:364, SIMILARITY:Drosophila FT melanogaster, Q9VHJ2, cg11975 protein., (340 aa), fasta FT scores: opt: 594, E():0, (35.4% identity in 243 aa)" FT /gene="SPAC458.06" FT /product="conserved hypothetical protein" FT /protein_id="CAB93848.1" FT /translation="MSTINTVSLNQDASCMSVALDTGYKIFQINPLKLRAQRQFNDGGL FT SIVKMLFRSNVLLLVGGGGNPKYAPNKLIVWDDVKERPVKELELNFEIKGICFDGKLLA FT IATASKLFLYQFGNNLKLQRCLDTQNPKGLCAMVTTVEKTAIVFPSRKVGQLQILFLFK FT DHMNTSIVPAHDSEISCLGISKTGSKIASSSTNGTLIRIWNSETGEKICEFRRGYQHTA FT VCQLAFSPDELLLACASKKETLHIFSLHGSPNTIRQLTSEEPYEEASEFKSSTTEPRQT FT HWKRKLLKLIDSGKRAHWRIQLYQSNPVLLHWLDEMTILICYKDAAYQKLKLTIEESSK FT SVEHANQHVCFHYDYTLEADGSLC" FT misc_feature 12029..12034 FT /note="gtaagt, splice donor sequence" FT misc_feature 12181..12186 FT /note="gtaagt, splice donor sequence" FT misc_feature 12318..12323 FT /note="gtacgt, splice donor sequence" FT misc_feature 12360..12373 FT /note="ctaacatatctaag, splice branch and acceptor" FT misc_feature 12466..12471 FT /note="gtaaga, splice donor sequence" FT misc_feature 12497..12514 FT /note="ctgacttttgaaatgtag, splice branch and acceptor" FT CDS join(13629..13873,13915..13961) FT /db_xref="GOA:Q9P3W1" FT /db_xref="SWISS-PROT:Q9P3W1" FT /label=SPAC458.07 FT /note="SPAC458.07, len:372, SIMILARITY:Saccharomyces FT cerevisiae, T2EA_YEAST, transcription initiation factor FT iie, alpha subunit, (482 aa), fasta scores: opt: 333, FT E():1.2e-12, (26.0% identity in 411 aa)" FT /partial FT /gene="SPAC458.07" FT /gene="SPAPYUG7.01" FT /product="putative transcription initiation factor TFIIE FT alpha subunit" FT /protein_id="CAB93849.1" FT /translation="MSNAPEIVQRLIKMIMRAFYETRHIIFMDAILRHSAYVLHKIRTI FT LTFGRLTDEQTALLMGIPIKECRFIAGKLREDRLLAIQSRTEMKEGQQRQYH" FT misc_feature complement(13743..13756) FT /note="ctgatcttgtgaag, splice branch and acceptor" FT misc_feature complement(13778..13783) FT /note="gtaagt, splice donor sequence" FT misc_feature 13857..13961 FT /note="nominal overlap with clone SPAPYUG7 S. pombe FT chromosome 1" XX SQ Sequence 13961 BP; 4512 A; 2404 C; 2388 G; 4657 T; 0 other; gatctaaata tttatccttc atttgatacc ttgccaaagg agctttacga aaaaatgcct 60 cctactttat ggccactacc aaagaaagaa cttgctgagc tgaatattca aaattgctta 120 cgtatttatc cacatttcca aaatactgga ggcttttttg ttgcagtttt agaaaaatat 180 gaaaatctta ctagctctat gaaaactgcc gtcgacgata acaaggtttt tttacgagaa 240 caaaagttgc caagtgaaca agcatcaaaa aaacgtaaac aagatactca agaaacatct 300 tcggattcaa aattagccga agttaaacca aaaggaaaaa atgggggcaa tcgatttcac 360 gaattggatc ctttcgtgta tataaaagag gacgatcaag ctctcgagaa aatctataag 420 aagtttggca ttgatgaagc tatcataaag aagaatcagt ttttcgttag aaatgtcaat 480 ggtgtcccta cgaaagccat ttatatttcc aacgatcttt tcagaaacgt aattgagaac 540 aatcgtaatc gtgttaaatt cgtgcatggt ggtctcaaaa tttttgtgag acaagatttt 600 ggaagtcttt caagagagat agcagaaaaa aacggtactt gcgtttttcg agttcaaagc 660 gatggagcga atcttgcttc tcattttatc gctgaaagct gtttgtttca tactacatta 720 tctgatttgt tcattttatt agaccatgaa gccgtgacca ttgacgattt tcctgaagac 780 agtttgttca ggaaagaata taaccatttg gatcttggaa gcactctact ccatgttgat 840 ttggcaaaag aagaaagcgt tataaagaag caagtctaca tacctttatg gaaaagtgtg 900 cgtatttgca acgttttgtt atcgaattca gaaaaaaggt aagaacatga tatatacgca 960 gaacctattc taaccaaaac aggacattaa aattacagat agaagggcca cagagttcaa 1020 tacacaaaca taacacttga acctgaagtg tcaatactac gaggatagac aaaatactta 1080 tataaatata ctgtaaagtt tctgaataga aataatggga atacttgaat gttctcaatt 1140 caaaatgatt gtatacaaaa ctattaaagt catcagccaa ttcacttaag aaacttaatg 1200 acagagatat aagaattagc tacaacctca ataactttaa gctgtagctt ccacctccaa 1260 atcattagta actgtcacag tttcttcaga tgatataacc atatcagctt cagttaaagt 1320 tttttcttct ttagaattat atttagcttc cagtttttca atttcctgct tggctttctc 1380 aatacgttgt ttggtaacag agtcttgctg ctctttaaaa tgggcgatac gtgacttaag 1440 ttgttcaacg gctgaatcaa cttgagactt agtgaaagga gcaggaacac cgacaaaatc 1500 gaattcctta atagtgccaa aagaaagatt gagtctatca gaatcggctt gattgttgtt 1560 gcttttgttt gattttttgg gcttcgattt tttaagtccg ctaaacatgg catcatcgga 1620 agactctttc ttaataatag ttccttcagg aattggatcc acagtgcgag gagtcaaagt 1680 tcgtggcttc aaaattttac tagaagtatt tccggttgaa acggcatttg tagtagaaga 1740 ttcgacggga acatgaaaca cctttaataa gttttcacag gcaagaatct cttccgtaaa 1800 ggcaggaata ctagcctctt ctaacttgcg ttgagcagca attcttctct tctccttctc 1860 acgattttca cgctcaagac gaaactgttc ttgtcttttg gcacgttgtt cacgttcata 1920 agtacggaat gcgcgcaatt gggtatcgta ctcacgttgt aaagctcttc gttgattata 1980 tagatcatcg agtgcagtct tctcatcatc tctttctgct atgagcttac gctgatcttt 2040 atagtaaccg tcttgttgct tacgaacctc atcaagctca gaacggattt ctacgaactt 2100 gtcactcaac ttctttgatt ctgaatcatt aagttgatca cgaagctcgt taagttcatt 2160 tctaatcgta tcaatactag tttgaagagc gttcaactca acgaagctct ttctagtacg 2220 attgcactga gaaatttcac gcaggtattt cttctcatca acaatcttta aagttcctga 2280 gtcaacggcg gcttgaagtt gtttaacatg tttatcaagc tccacttctg acttgaaagg 2340 aacagttttt ttcatggcag tcaactcctt aacctaaaag tatgtcagta aacattttgt 2400 ttctttacca agctcataaa gttttccaac acaaaacttt agtgcttaca tatattaatg 2460 caaaacatac cttctttttc aaaagttcat cttgagcctt gactttgttg atgagagttt 2520 gcttagaaga acggatagct gcttgagcat tgcgaatatt gtctaattca gcgcgaagtt 2580 ctgcattctt ctcctgcaat gaaccaccac cgcgaatggc accaagcttc tccttatgct 2640 cattctagtt gattagttaa gatgcgaaaa ctatataaga aacaagtgat tttttgaagc 2700 tttcatagtt atgaaaaaaa aaaaaagctt agccttcaat attcaagaat ttaaaactac 2760 ttacgaaacg ctttttcgcc tcgttgatct tagcatcaag ctcctccaaa gcctccttgt 2820 gaagagcttc attaggacga acgggtttat gagatgacat ttttattaag gattttgttg 2880 ttggtggtgg atagtggtgt gtgttgagtt gtcgtttaac gagtatataa caaggcagat 2940 aaactccgtt tgttttgctt ccctagagtt ttcgttcgca gtgctgtgaa ggagactacc 3000 tgtttctgaa atttttataa atagcttttt gttgacggtt attcagacat cgtgtgaaga 3060 gcagtctaag gaaaagatta cgataataca tgttatgata tttttttttt atttatttaa 3120 ataaactggt agttttttgc actcgtccac gtattatgtt taatatttca atttaaaaat 3180 cctgaattgt aaatttagtg acctttattt gcaatgtgtt gacttgaacg aaacaagaaa 3240 aattaatgca ttttttctca cggattagct taccgattag cggagcatta acatttaatt 3300 tgggaggtac acaaaaacag ttttaaacta aaagttactt gctttttaaa agcaaataga 3360 aatattgcaa ctagtcttat aaagcaacag gatacttcac ttatagaaag cgaatttgaa 3420 agcataacta tccattcttt tgttacaact attgcatata agcacaaaat gtctttaagt 3480 taaattatac ataatatcct ttttctgtca cgtaaattgt aagttcctat tcaatttcag 3540 gctgtgaaaa gtccgtgaaa ataatacatc gaagagcttt acaggtttca cagaatatcc 3600 cgatgctacc catgtaattg ttgcatacac cattctcagg aagtttaaga gcacaattta 3660 aaaaactata tgaattgagt tcaaattacc tttatagaat gaaaaacaat taatagaatt 3720 taaagtaatt tacatggaca acaaattaaa aataactata tactttgtaa agagttttgg 3780 tgttgtttta gccaagcagc ctataatcct gcatattgct gattgctgac cggtgaatta 3840 atccaccgaa ggttactgtc ttctcaacac cccaaaataa ttcggttatt tttcaactcc 3900 aaggatatga aatcctttgc tagcgaacta tccaaaccta ttcaaaagcc gcgtttaaaa 3960 gaaatcctta aagaactgca gcatataggt gttccttctc cttgcaatct aagattatat 4020 catgagttaa ttagtgtgat agtgcccaca ttctggaatg ggaaaattaa ttcagaaata 4080 gattttctcc ttgcaaaatg tttttctact cttttgggct taaaactgct atgtgttcga 4140 ctagataatt ttgttcaaga ggctaatttg caaaacattc atcttgttac agtttatctt 4200 gagctactgg aaaaagcatt tgaatgtata gaccttgacg ataactgtaa attgatatgg 4260 gaatctactg tattatctga tcatcaaaag caatctttgt ggactgaatt tatttttttc 4320 ttgggaaact ttaaaattac taattcaatg tctgctgcaa tgcttacctt caaggtttac 4380 gatgattcaa aacgtttgaa ggcttcatca atggccgact acattggcgt tttatcttca 4440 aaattggctt gcattatatc taaacctgct gttaaaaccc cagaaatata ttcaaagcta 4500 ttccattatt tattacactc atccaattta aaggcattca ttaacccatt gattccatta 4560 acacaaaaat tttgtgttca gctacaaaaa ttatttgcag atttgacggt ctctgatcaa 4620 atgttgtttc tcaatcaact tcttcttgaa cataatacca aatatcccac caatttttca 4680 tattccaccg caagagatga tcgtataaca ggttcattgg ctacattatt aaggctgaac 4740 ttttcatcca ctcacttcct tcgattaatt gaattttatt ggggcgtccc tacaaattta 4800 ataattaaaa gggtagaagt agtttgttcg agtatatctt ataaggaatc tgataaaaag 4860 gagctagaaa aaacagtgga ctctctgttt aatatatggt caaatgcgca atttttgagg 4920 agctatacgc tgctagctca agagagctta acaatatatt tattactatt tattccgaaa 4980 cttgaagcaa aatatttgaa taatctttcc aaatccctaa tgttctccaa tgcaatatct 5040 tgccgtttag atagtctaga tgatgatatt cgaatgcatg gaatgataat ggccgaagta 5100 atctctacat attcgggtgt cagcttaagt aacccgttag cctttgacgt tccaataatg 5160 aagactacga aagcaaaagt tctcaaatcg ttgagctcac tcaaggatga atttttgcct 5220 attgaaattt tgaatgacga aagtgttatc gcagagactt cgatagaaaa ggaagagacg 5280 aatgtaactc acttggagaa gccagtgatt tctaaaaacg atgacttaat aaggggagat 5340 gacttggagc cttacgattt tcctgatatt gacagtgagg acacggacga cgatccaact 5400 gtctctagat ccaagacgca ttctcctgtg tacgtacaag atttgtgcaa aatgttaaaa 5460 gacacagaaa gttttgagaa acaacgtgtt gcactggaaa acgcttctaa gttaataaaa 5520 cgaaagtctg cttttggaac cgaattacga gatcatgcag atgaattgtt acaaacgtta 5580 atttccttac aaaatcgatt tgacttaatg aactttgatg aaatgcaaat gactgctata 5640 gtggaacttc tgttaacctg tttagatata tgtggtcctg ttatttgtac aaatttattt 5700 gttagtgact attcaatgcg ccaaaaaata cttatcttat cttgtatttc tttagctgct 5760 agcaaattca acgatgatga taatgaacgt ctttttccca gccagttact tccaggaaat 5820 ttacacgatc agttttattc acccactatt gaaaagatta gtgatgaatt agaaaggaaa 5880 ttagtttttc cggtaatgag tgaatgcaag gattatgcgg agggaccgaa attcttacag 5940 acacggtata tttcaaagca gtcggaaatc gagtcgaaaa agccactccc aaaggccaat 6000 cgattatcgc ttaaggtaga taaatcattg ttcctgctcc tcaccagtgg ttatatgctg 6060 gcatctagga aaacggcttt tattgagcct ttgtttttag tctactatct taagacattg 6120 ggtattctct tttttgaaac ttttacctca aagatagaag gggcaaacag aatgctccgg 6180 gaatatgttg acgttttaaa tgaggtctgc cgacttcgat cagacactcc agaagttaat 6240 gaagggcttt tgtttagttt tttggctatc ttagaaatat cttctgggcg tactttagct 6300 aatgagtttg ggaagcattt gctcttcttt gaggcgtata ctaagtttct ttttgaaagc 6360 cctgatcaag gtgagaaagt taagtcactc tcagcagctg ttttaattct atttgaaaat 6420 aaactttcag aattcagaac gctcgcattg gaaaaattac tggatgactc agctcctttg 6480 gttccagagc gatttggatt agccggactt taatagttct ttatggtcat gatttaaaag 6540 aaaaaaaata ttatttatga ggttgaaatt ttgacagaaa ctcattaatg cattttaatt 6600 aaataatatg attttgtaat aataatgaat gtctttaggc caattacaat tattgaatta 6660 agtccttcca caaacttgat tttatggagg atttatttaa tttgtttgaa gaatacgttt 6720 taatctcgct atacttgttc tgtagttctt gcaaaaaaac ttggtgcgat ttgtagggta 6780 tatctccatc cgatattttt gaggaatgac agttcaacat cgatatactc ttatcaaata 6840 aaccaagtaa taaacttatg tcaagagtat cgtaaaaatt tttagcggtt tgtttaaaaa 6900 gggcgatatt aaattcatga ttgacactct gtattttaga gatagtgtcc caacaaggag 6960 gtataaacat agtttctgat tgaattgttt gagcatgaaa atctcgaggt ttaaactttt 7020 gaaacataaa attatacttc attaaaacgt tgagattatt ccagcttgtt gggtccgctg 7080 agatagaaac aattgaggac gttggtacgg ataaaagtgt cgttcgcaaa aactgtaaaa 7140 taaattcctg tgaattatcg actgaggtat gcaagcctaa agcatgcttc aactacaaat 7200 tagtaaatgt gcaagtattc aagggtatct tacctgtttc agaacaaccg taaagtttag 7260 tagaatacca ctacttatat ccaatgttgt cgagttctta tccagtaaag ctaccagttt 7320 tttgttctct aaagcagatc agttaacagg ataattgagt tcaatcaatc ttacgagact 7380 cttcaaatcg attcctcaca gtttcctcgg tatttataag aattgttttc atattccgaa 7440 gtatatttaa taacttctcc ggaaaggaaa ctaacgcgga aagagtctgc gctacgacca 7500 ttattatcca taacagatcc aatccatgct cgtttaaagc ttgactaaca agaaaattga 7560 atagtccacc agttaaatgt gaaatttgcc aaaagtttac tagcagcatg agataattgg 7620 ttaatcgtac taatcgcgta cctttcactt gatcctttgt aaagaaacac ttataagcaa 7680 ttcctcgaaa atttacagtt tcacaatcat ttgataattc tgaataactt cccccgctaa 7740 aacatccgtg ctcgttcata cattgtataa tatcgaggtc ttcacctatt catgataaat 7800 aagaatttca atatggagcg tacctatgca tataatacta catgctttcg gttccttctt 7860 tttgagatcg tttagcaact tttccaataa ttcatccata tgcaattatt tatgtagttc 7920 actaataaaa gtgttcaggc acaagtcaaa gacaaacgat taaactttag ctaaaaggga 7980 tcatttagat gacgaagttc agatctcaag tttattataa tacaatgaaa atcgttatat 8040 ttttacaagt actagtatgc tagacatatt ctttaataat gtatctgccc gaggttaaag 8100 caaaatcttc ataatatata gaattaaaat aaatgaataa aattcccact caactttgtt 8160 ttaaattggt gaagtaataa tgccaacagc ttttgagtca ctttcacgat aaatttgcaa 8220 aagcgaccga taagagaatc ccgcaaattg aagtcaatta agatttaaat ataatttttt 8280 atgaatttaa aaacaggaaa atcttatgag aaaattccac tggtttacgc agacacgtat 8340 tttttataaa ctgatactaa aatagggcct ctataaaata attagtttta ttatgaaaga 8400 ttaataatta gttcgttcta aattttacaa ttaaataaaa atttaaaatt atcagacttt 8460 taatatgctt ttgctccgaa tattatatga atatatgaca gtaaatctac aggcttgtta 8520 ttttatcgaa cttatttttt taaaaaatta ccagataaaa taaaatatta aaacattcat 8580 ttttatcatt taatgttatt tacgttcact tttaagcaaa ttgttaggtc ttcttttaca 8640 acaacgttaa ttcataaaac tgtatttttt atatttcaag aaagtgtgaa tttatatcaa 8700 ttattgcaaa agattatgtg ctgttgaatg aaccacatag ttgatcgtat tgttaaccaa 8760 gcagcaattg ttgcttagtt cctagtcgtt ccggtgcttg aaaactaaaa ataattaatt 8820 caatgtgagc aaacacaaaa cataaaacac cattagcgca tagagggtat aggtgatcaa 8880 ttattcagag cagttttagc ttcttcggct ttaccattta ttccgcctca agctttgctt 8940 acagtgttat gttcagacac tcaaaacttg actttacatc gagatgtaga gatgaatgcg 9000 tcctattttt tgtatttcaa aaattacagc aaaaacactt gtgtagtaga ttatgagata 9060 ttattattat tataccgtat tcagcatttt gtacttattt tacgaagtat ttcttaaatt 9120 ccacagtatt ataattcatc attgcttcgt gtactttagc tacagccttt cttgtcttac 9180 cacatttagt aaagctagta caccaccaca ttaacaatcc caggctatta tttgtatggt 9240 aaaaagtgaa atttccaaaa aataaatgct ttacatatgg aatggataga cttgtttttt 9300 catattgtcc ttcaagcaag gtgactgcca gattcttggt taaattttgc ttcattgaat 9360 atcaagatag tcaagaacca tgcatttgca ccattcaatt gttttcaggg aatgaatcag 9420 gatctttaat gcaaaaatgt ttcgtttcaa aaataccaaa taagagtcta cttcctactg 9480 agctttctaa gatatcaact catgagtggc tcgactttgg ggttaccgtt tctgagctct 9540 cattgaacgc aaaatttgta gtgtctgcct ggaaaccatc ctttaatgat gaggaagtct 9600 atgaatttgt gggctgtaca acgtatcgac tttttgatga aaataattta ctaaggcaag 9660 gtctccaaaa aataccttta cagacttcaa aagaaataaa gaagtactcg ccaacatcgt 9720 tggaactaga acaagttaaa gaaataaacc ggcttgacgg tttacttctc aaattacagt 9780 tgggcgatgt tccatccgtt aattggctgg atgatatatc ctttggaaaa atcaaagact 9840 ttagatctaa gcacatgtct ctcgtcacta tccctatttt gtatcttgat tttcttcaat 9900 tctctttccc cgtcgtcttt caacgatcct actatcccaa atctgaaaat cgagtatatt 9960 actcgtcctt tgatttggag ttaaatctag atagtccagc tgagctgaaa catagacgac 10020 tggttcgttc tcaacgtaat ggacctttgg ataaggactt aaaacctaat tcaaaaattc 10080 gaaaagagct tgagtcaatt ttatcttatc caccaagcga agaattatca cttgaagaaa 10140 aggaccttat ttggaagttt agattttatc ttaccaggaa taagaaagca atgactaaat 10200 tcttgaaaag cgttgtttgg actgattcat ctgaagtcaa tcaagcactt agtcttttag 10260 atagctggac cgaaattgac attgacgatg cattggaatt actaagtcct tcatttgtac 10320 accctaaggt tcgtgcctac gctgtctctc gtttagagac tgcttcgaat gaagaattgt 10380 tgttatacct tctccaactg gttcaagcat tacgatatga caaccctatc agctctgatg 10440 aaaggttcca acctagccct cttgccttat ttcttgtaaa tagagcaata tcatcaccga 10500 gtatcggaaa tgatttatat tggtacttag tagtagagat tgaagatgag cccgtaagca 10560 agctattttc ttccgtcatg tttctttttc aaaaagagct ttcaaagtct gtggaaggaa 10620 gattgatccg cgaaacactt tctgctcagg caaaattcgt tgaaaaatta ctacgcataa 10680 gtaaatctgt acaatcattt cgcggtactc gattgaagaa aattgaatac ttaaaagtcc 10740 ttttagaaga tcataagtat catttactgg attttcatgc tttaccactt cctctagatc 10800 catccgtaaa tatagttggc attattccag atgcctgtac ggtattcaaa tctaccatgc 10860 aacctttacg cttacttttt aaatgccaag atggatcaaa atatcctata atatttaaaa 10920 acggtgatga tttacgtcaa gaccagcttg ttattcaaat attaacactt atggataagc 10980 tgttgaagaa agagaaattg gatttgcatt taaagcccta tagaatcctt gcaacaggtc 11040 ccactcatgg tgccgtacaa tttgtaccgt ctaaaaccct tgctactatt ttagcagaat 11100 atcatggtag tgttttagct tatttacgag aaaacaatcc agatgatgga ttaaattctg 11160 cgaattacgg gatagaccct gttgctatgg acaactacgt tcgaagctgc gcaggttatt 11220 gtgtaatcac ttatttatta ggagttggtg atcggcactt agacaatctc ttgattacga 11280 aagacggtca tttttttcat gctgactttg gttatattct gggtcgtgat cctaagcttt 11340 tttcaccagc tatgaaactt tctaaggaaa tggttgaagg tatggggggc tacaattctc 11400 ccttttatca gcaatttaaa tcctattgct atacaacttt tactgcttta cgaaaatctt 11460 cgaacctgat ccttaacttg ttttcgttga tggttgatgc gaatataccc gatataaaat 11520 ttgacaaaga aaaagtagta tataaagtca aagaaagatt ttgtttacaa atgtctgaat 11580 cagatgccat caaatatttt gaacaattaa ttaatgattc tgtcagtgct ctgtttcctc 11640 aaatcataga tcgcatgcac aatcttgcgc agtatatgcg atcctaaaca ttaatgactt 11700 aatgattata tgcccacatt tacacttttt tttttatcca aacttgcttt tccattatag 11760 ctgttgttga actaatacat tattatgcct agtaaaaagg ttgtgttttc taaagaaagt 11820 gatgaattgt ataattgacg gtgtgtaatt taccgaattc accgagaaca atctaaagta 11880 gttttgcata atgcataaac cccacaaatt ggaatgtcaa ctattaatac tgtatcatta 11940 aatcaagatg cctcatgcat gtctgttgcg ctagatacag gctataaaat ctttcaaatt 12000 aaccctctaa agctgcgcgc tcaaagacgt aagttttgca atatgcagat ctcttatttg 12060 aatatttaca attgtctaga gttcaatgac ggtggtcttt caatagtcaa aatgcttttt 12120 agaagcaatg ttttactctt agtaggcggc ggtggaaatc caaaatacgc accaaataag 12180 gtaagtttga gtaatgcttt gttttctttt agctaagctt gtttacagct tattgtttgg 12240 gatgatgtga aagagaggcc ggtaaaagag cttgaattga attttgaaat aaaaggaata 12300 tgtttcgatg ggaaattgta cgttcttctc ttttatattg cttgtcgtct tattaacaac 12360 taacatatct aagattagca attgcaaccg catcaaagct atttttgtac caattcggaa 12420 ataacttaaa actacaaaga tgccttgata cccagaatcc aaaaggtaag acattctttg 12480 cttagcatag tcgaagctga cttttgaaat gtaggcttat gcgctatggt tactactgtt 12540 gagaaaactg ctatcgtttt tccttctaga aaggtcggcc agctgcaaat attatttctt 12600 tttaaggatc atatgaacac tagcatagtg cctgcacatg atagtgagat atcatgtttg 12660 ggtatatcca aaactggttc aaaaatagca tcctcttcaa caaacggtac tttaattcgt 12720 atttggaatt cagaaactgg agaaaaaatt tgcgaatttc gtcgtggcta tcaacacacg 12780 gcagtttgtc aattagcgtt ttctcccgat gaattattgt tggcttgtgc aagtaagaaa 12840 gaaactttac atatattttc tcttcacgga agtcctaata caattcgtca attaacgtct 12900 gaagaacctt atgaggaagc aagtgaattt aaatcttcta caaccgaacc ccgtcaaact 12960 cactggaaac gaaaattact taagttaatt gatagtggaa aacgtgcaca ttggagaatt 13020 cagctttatc aatctaaccc tgtactttta cattggttag atgaaatgac tattcttatt 13080 tgttacaagg atgctgcata tcagaaatta aagcttacga ttgaagaatc ttccaaaagt 13140 gtggaacatg ctaatcaaca tgtatgcttt cattatgatt atacactaga ggcagatggg 13200 tctttatgct gatgactttc gatttctttc tagtacttta cctttctaca ttagataatt 13260 ttgtatataa cttttcaaat aacgtctcat taatgatttt atgttaaaca atgatactat 13320 cctaattgtg catgaatttt aatacgtatc aagttttagc tagttcaccg aagaatttat 13380 tgaacaacgg acattgtgaa taaatttata gtaatttaag gcaatggacc acttttaaga 13440 ctattatgtt tgaatgtcat tttaaaagat gttttctaaa ttaacagtct tcatgactat 13500 aggcatttaa agagtcatta gttaacgcct taagcagtgt ctttttgtgg tacattctcc 13560 actactccat caccctttat tattcgctgc aaaaaccttc cttcgattta tcgtatcatt 13620 tatctactat gtccaatgct ccagaaatag tacagagact tataaaaatg attatgaggg 13680 cgttttacga aactagacat ataatattta tggatgcgat tttgcgacat tcagcgtacg 13740 tgcttcacaa gatcagaacg attttaacct ttggtagact tacagatgag caaacagcgc 13800 tgttaatggg tatccctata aaagaatgtc ggtttattgc tggaaaattg cgcgaggatc 13860 gtttattggc aatgtacgtg aaggcaattc catgaccagc tgctaacctt tcagtcaaag 13920 taggacggaa atgaaggaag gccaacaaag gcaatatcat a 13961 //