ID SPAC25A8 standard; DNA; FUN; 6638 BP. XX AC AL021809; XX SV AL021809.4 XX DT 08-FEB-1998 (Rel. 54, Created) DT 31-AUG-2000 (Rel. 64, Last updated, Version 4) XX DE S.pombe chromosome I cosmid c25A8. XX KW helicase; SNF2. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-6638 RA Skelton J., Churcher C.M., Barrell B.G., Rajandream M.A., Wood V.; RT ; RL Submitted (06-FEB-1998) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Hinxton Hall, Hinxton, Cambridge CB10 1RQ E-mail: barrell@sanger.ac.uk XX DR GOA; O42861; O42861. DR SPTREMBL; O42861; O42861. DR SPTREMBL; O42862; O42862. DR SWISS-PROT; O14138; YFC3_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c25A8 is ovelapped at the 3' end by cosmid c3C7. XX FH Key Location/Qualifiers FH FT source 1..6638 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c25A8" FT /map="IR" FT misc_feature 1..2 FT /note="nominal overlap with cosmid SPAC3C7, EM:Z99568 S. FT pombe chromosome 1" FT CDS complement(1..212) FT /db_xref="SWISS-PROT:O14138" FT /note="SPAC25A8.03c," FT /partial FT /gene="SPAC25A8.03c" FT /product="hypothetical protein" FT /gene="SPAC3C7.15c" FT /protein_id="CAB61874.1" FT /translation="MLNLYMWKLSRSQVYQASLLFGSRVISALAFTNTGLVLHGPRRWY FT TTDNGFLLHRDSKVSLADYIHESTF" FT CDS complement(708..3476) FT /db_xref="GOA:O42861" FT /db_xref="SPTREMBL:O42861" FT /label=SPAC25A8.01c FT /note="SPAC25A8.01c, len:922, FT SIMILARITY:Schizosaccharomyces pombe, O74842, putative FT helicase., (1284 aa), fasta scores: opt: 2644, E():0, FT (45.9% identity in 937 aa)" FT /gene="SPAC25A8.01c" FT /product="SNF2 family helicase" FT /protein_id="CAA16951.1" FT /translation="MDGKRKIEHTADGTHYDATSNVKRKPIFPPFIADSLSEATEKANV FT MGGGMNSRLQILSEMSKRVQATAPISSLEHFKQLSDISPSFTSSANSINQPYNYSGSLE FT NLVPTPSAGTPSQFMDAQNPYGAVYNALSQFSETEPKMPSYMDDEEASDSLPLSLSSQS FT LSSQVTNQKPAPHRLTMRERYAANNLTNGLQFTLPLSSRKTYEPEADDDSNDDMYSDDD FT SNADRWASRIDTAALKEEVLKYMNRCSTQDLADMTGCTLAEAEFMVAKRPFPDLESALV FT VKQPRPVIPKGRRGRREKTPLGPRLVGICMEIMRGYFVVDALIRQCEQLGGKIQRGIEA FT WGLSNTATSDEGETSLVNFDQMKSFGTPANSSFITTPPASFSPDIKLQDYQIIGINWLY FT LLYELKLAGILADEMGLGKTCQTIAFFSLLMDKNINGPHLVIAPASTMENWLREFAKFC FT PKLKIELYYGSQVEREEIRERINSNKDSYNVMLTTYRLAATSKADRLFLRNQKFNVCVY FT DEGHYLKNRASERYRHLMSIPADFRVLLTGTPLQNNLKELISLLAFILPHVFDYGLKSL FT DVIFTMKKSPESDFERALLSEQRVSRAKMMMAPFVLRRKKSQVLDALPKKTRIIEFCEF FT SEEERRRYDDFASKQSVNSLLDENVMKTNLDTNANLAKKKSTAGFVLVQLRKLADHPML FT FRIHYKDDILRQMAKAIMNEPQYKKANELYIFEDMQYMSDIELHNLCCKFPSINSFQLK FT DEPWMDATKVRKLKKLLTNAVENGDRVVLFSQFTQVLDILQLVMKSLNLKFLRFDGSTQ FT VDFRQDLIDQFYADESINVFLLSTKAGGFGINLACANMVILYDVSFNPFDDLQAEDRAH FT RVGQKKEVTVYKFVVKDTIEEHIQRLANAKIALDATLSGNAETVEAEDDDD" FT misc_feature complement(1095..1346) FT /note="Pfam match to entry helicase_C PF00271, Helicases co FT nserved C-terminal domain" FT misc_feature complement(1632..2549) FT /note="Pfam match to entry SNF2_N PF00176, SNF2 and others FT N-terminal domain" FT CDS 5209..6381 FT /db_xref="SPTREMBL:O42862" FT /label=SPAC25A8.02 FT /note="SPAC25A8.02, len:390" FT /gene="SPAC25A8.02" FT /product="hypothetical protein" FT /protein_id="CAA16952.1" FT /translation="MLHVNKCQLCETRRPSICESCLKKQLYDFYHKRDEFSRDIESELE FT KVAKLKSESFSLKGKITQKEELTYRLQNLAASLNEKKSLSCDLHSRKQLIEDDLSNRKK FT SINIASQKLAGLSHSTSDYFSKEYLTAYRRSELLQNKLHEYQKKLITRVLDMYQLHWQR FT DLVLNTQGCTQKSAQMGSEFNPDSIDSSLAYRLSKLSMGNSKSDLSHSDNETGHFYSNF FT FSQVMELNASVTISGIPVCIRSKEKMFLNPDCALTLSFICIFLAQYTSIPLPCPLQLPS FT PDQKPMTSFSSEQMLYIMYNIVWISWNCGIFSFPRGITQSQLFELMQYTGFWLVQLRTK FT PLTSQKPDWHYKMGMDFDLFHRLYLARLPIPINYSSLRSRSSSKPYQLIS" FT misc_feature 6535..6638 FT /note="nominal overlap with c631 S. pombe chromosome 1" XX SQ Sequence 6638 BP; 2182 A; 1173 C; 1169 G; 2114 T; 0 other; tcaaacgtag attcatgaat ataatcggct aacgaaacct tagaatcgcg gtgcaacaaa 60 aatccattat cagtagtata ccatcttctt gggccatgca aaactagacc ggtatttgta 120 aatgcaagag cactaattac tctgcttcca aataataatg aagcttgata aacttgagag 180 cgacttagct tccacatata aagattcagc atttaacact ctttgtagta ttaaaattga 240 acaaaattca ttatttattc gattactatt attcgaagtt gagaaataaa atgtaataat 300 atgatttcat aaacttacta ggtgaagtgc tttcctctgt caacttttgt tttgcaagtt 360 ttagaacgac tttggataat aagaatcgct tatttgctgt attatcttca aaaaagactg 420 accattttca cgacgcgttt ttggcgtggt atataaatgg tgtcaagaag ctaaaagctt 480 tcatattagg ttgaagaagt actgaatttg tatcattcaa taaatagaat ttacttttac 540 aaatcaaatc ttttaatctg atttagtgaa acgggatcga cttataacgt gcaaaacaca 600 aatacagata atacaattga aaataaagag aaaggtttac tcgaataaag tagaaaccta 660 atcaatttga ctattttaaa actaatgcat aaaaaaccct gtataaacta atcatcgtca 720 tcttcagcct ccacagtttc ggcattgccg gaaagagtcg catccaaagc aattttagcg 780 ttggctaaac gttgaatgtg ctcttcgatg gtatccttga caacaaattt ataaactgta 840 acttctttct tttggccaac tctatgtgct ctatcttccg cctgaaggtc atcgaatgga 900 ttgaaagaga catcataaag aataaccata ttagcacacg caagattgat accaaaaccg 960 ccggctttgg tagaaagaag aaatacatta atgctttcat cagcataaaa ttgatcgatg 1020 agatcctggc ggaaatcgac ttgtgtagaa ccatcaaatc ttaaaaactt tagatttaat 1080 gatttcatga caagttgaag aatgtccaaa acctgtgtaa actgactaaa taatacaact 1140 ctgtcgccat tttcaacagc attagtgagc agttttttaa gttttctgac ctttgtggcg 1200 tccatccaag gttcatcttt aagttggaat gaattgatag acggaaattt gcaacacaaa 1260 ttatgtagct caatatctga catgtactgc atgtcttcaa atatataaag ttcgtttgcc 1320 tttttgtatt ggggttcatt cataatagct ttcgccattt gtcgaaggat atcatcttta 1380 taatgaattc gaaacaacat agggtgatcc gcaagcttac gaagctgtac aagcacaaaa 1440 ccagctgtgc ttttcttctt tgcaagattt gcattcgtat ctaaattagt tttcatgaca 1500 ttttcatcca ataaagaatt tacactttgt ttgctagcga agtcatcata acggcgacgt 1560 tcctcttcgg aaaattcgca aaattctata atacgcgttt tcttcggcaa agcatctaac 1620 acctggctct ttttacggcg aaggacaaat ggggccatca tcattttggc tctcgatact 1680 cgctgttctg aaagcaatgc tcgttcaaag tcgctttctg gggatttttt catagtaaaa 1740 atgacatcta aacttttcaa accataatca aatacatgag gaagaatgaa cgctaaaagc 1800 gaaattaatt ctttaagatt attctgtagc ggtgtaccag ttagcaaaac tcgaaagtcg 1860 gcaggaatac tcatcaaatg acgatatctc tcagacgcac ggtttttcaa gtaatgacct 1920 tcgtcatata cacaaacatt gaacttttgg ttgcgcaaaa acaaacgatc agccttacta 1980 gtagcggcta aacggtaggt ggttaacata acgttataag aatctttatt agagttgata 2040 cgttcacgaa tttcttcacg ttcgacttgc gatccataat aaagttcaat ttttagcttg 2100 ggacagaact tggcaaattc acgtaaccaa ttctccatcg ttgaagcagg tgcaattacc 2160 aaatgtggcc cgttaatatt tttatccatg agtaatgaga aaaaagcgat ggtttgacaa 2220 gttttaccga gccccatttc atcagccagg ataccagcca gtttgagctc gtacaaaagg 2280 taaagccaat taatcccaat gatctgataa tcctgtagtt tgatatcggg cgaaaaagat 2340 gcaggaggtg tagtgataaa cgaggaattt gcaggcgtac cgaaactttt catttgatca 2400 aagttgacaa gagaagtttc accttcatca gatgtagcag tgtttgacaa gccccacgct 2460 tcaatgccgc gctgaatttt accacctagc tgctcacact gacgaataag cgcatccact 2520 acgaaataac cacgcataat ttccatacat ataccaacta aacgtggtcc aagaggagtt 2580 ttttcacgtc tacctcgtct acctttggga atcaccggac gaggttgctt aacgaccaaa 2640 gcagattcaa gatctggaaa aggtcttttg gcaaccataa attcagcttc agcaagcgta 2700 caacctgtca tgtcagccaa atcctgtgtt gaacaacgat tcatatattt aagaacttcc 2760 tcctttagag cagcagtatc tatacgagat gcccagcgat ctgcatttga atcatcatca 2820 gagtacatat catcattgct gtcatcgtca gcttcaggct cataagtctt tcgagatgac 2880 aatggcaagg taaattgaag tccgttagta agattattag cagcatatcg ttctctcatt 2940 gtaagacgat gtggggctgg cttttgattt gtaacctgtg aactcagtga ttgagacgat 3000 aatgaaagag gtaaagaatc gcttgcttct tcgtcatcca tgtaagaggg cattttgggc 3060 tctgtctcag agaattgaga taaagcattg taaacagcgc cgtatggatt ttgggcatcc 3120 ataaattgcg aaggagtgcc agcagaaggt gtaggaacaa gattttctaa cgaaccggaa 3180 taattatatg gttgatttat ggagttagca gaggaagtaa atgagggaga aatatcactc 3240 aactgcttga aatgttctaa acttgaaatg ggtgcagtag cctgaactcg tttagacatc 3300 tctgaaagaa tctgaagtcg cgaattcata ccacccccca taacattagc tttttcggta 3360 gcttctgaaa gcgaatcagc aatgaaaggg gggaaaattg gcttgcgctt cacgttggaa 3420 gtcgcgtcgt aatgcgtgcc atcagcggta tgctctattt ttctttttcc atccataatt 3480 aagtaacaat caatgtatcc aagcaaatgg caaaagaaaa ttgaatctaa aatataggat 3540 ttggagaaaa aatatgtaag gtcacgtgta tattatgaat aattttatca cacgaattaa 3600 gagcgccgag aatagatgca aaaagattga aactttttaa attctcctaa gattaattaa 3660 aatggaagaa ataacgacaa aacaatacag atttcttgtt tcacgcgttt cttggtaact 3720 caaatagttg aaaacttaat atgggaacag cagattacaa aaaattttga gggccaaaac 3780 ttattgcaat ttctaaaaca agaaacaatt cctttagtta cgagatgtgt atgagttaat 3840 taggatagac caccttcttc taatacataa gcgttgtttc gagatgatat gcagaattta 3900 atgaagaaaa ataaaatgta aatgaaaagt agacaaaaaa attttaaaat ataaatttaa 3960 aattttcctg atgtttaaga tgaagcaaac ttggtaagct ctagcaataa acaaatactc 4020 gcaattaaag atcaccaaaa acgacttata ctttggctat ccaaaaacaa tctttgttta 4080 aaaaaagaaa aagaaaatta ccaaaattaa aataataaca ctcaatgaat cggattgtta 4140 aagaaaaaga ctccagtcac ccaatacctt cttttgtatt atggaattat tcggtataat 4200 tcaagagcac actatgctgg tagtttgttc ttgatgtggc acgggggcgt gatatggtgg 4260 tagtggtccc aaatactgat gctaaataat gccttaaacg ttgtaacgta gtttatggta 4320 aagctatatc atgatggcaa cttgctcttt atattactaa acatttagca aaagataccg 4380 tggcgaaatt gtctttattc ttttatagtt ttaataaaat cattaaacat aactgcagat 4440 actagagatc ttttgtcact gaaccttaag aaatatgaca acaaatagtg tttcgttata 4500 ttatggcagc gtaccacgtt atgttttcta ggtgatactg caaaagctaa tgttaaaaga 4560 ggatggtttg ggttctagga ttatattatt tagttaatat tttattaacc tatttattta 4620 gataaaatag ttttgtaaaa caccttgctc atcactcaaa gttatttagc aaaaatttga 4680 gtgtcgagta acaggctact ttaaggaaat atttttaaac gctttataaa gtaactattt 4740 gcatacgatt gtagtaatca atacgaacat caactgctaa gtagctactt cggggatttg 4800 aagaaataga agtccatcta ctcattgcat tgaattcgta taatataatt ttttcttaat 4860 atttgtcgtt ttttctgtca aaaatatcgc attggttgtt caattctgca agaacagcca 4920 ggaatgcagt ttcactttta aattaatatt tttgaatttg tccgttagga ggcccttcgt 4980 ttctaagaat aattgtttaa aaacctgcgc tgtttatttt ttttcccttt ttcttctttg 5040 tttttgcttc attattttaa caacacctat attgtataca agttgacagt taacgtagta 5100 ggaaattaat atcctgctgg gtttttaagt tttcttattg ctgtgctata attgaaattt 5160 gcgtaccact tgaaggcatt gttggatcta aactgaaggt gtgtttgtat gctgcatgtc 5220 aataaatgcc aattatgcga aacaagacgt ccttctattt gcgaaagctg tttaaaaaag 5280 cagctttatg atttttatca taaacgtgat gaattctctc gagatattga gtcagagctt 5340 gaaaaggttg ctaaattgaa atcagaaagt ttctctctaa aaggaaagat tacccaaaaa 5400 gaggaattaa cttatcgact acagaacctt gctgcctctc taaacgaaaa gaaatcgtta 5460 tcgtgtgact tgcattcccg caaacagctt atagaagatg acttgtctaa tcgcaaaaaa 5520 tcaattaata tcgcgagtca aaagttagct ggcttatcac attcaacgag tgactatttc 5580 tctaaggaat accttacagc ttatcgccgt tctgagctac ttcaaaataa attgcatgag 5640 tatcaaaaga agctgataac aagggttttg gatatgtatc aacttcactg gcaaagggat 5700 ttagtgctta acactcaggg ttgcactcaa aaatctgctc aaatgggatc agagtttaac 5760 cctgactcca tagattcatc tcttgcgtat agactttcaa aattatcaat gggcaattcg 5820 aaatccgact tatctcatag tgacaatgaa acaggacatt tttatagcaa ctttttttct 5880 caggtgatgg agttaaatgc ctctgtgact atttctggca ttcctgtatg tatcagaagt 5940 aaagagaaaa tgtttttaaa tcctgattgt gctttaactt tatcctttat ttgtatattt 6000 ttagctcagt ataccagtat tcccttacca tgcccgcttc aactcccttc gcctgatcaa 6060 aaacccatga cttcattttc atcggaacaa atgttgtata taatgtataa catagtttgg 6120 atttcatgga actgtggcat tttttcgttt ccccgtggaa ttacccaatc ccagcttttt 6180 gagttaatgc agtatactgg tttctggtta gttcagctac gtaccaagcc gctaacttct 6240 caaaagccag attggcatta caagatgggt atggattttg acctttttca ccgattatac 6300 ctggcgcgtc ttccaattcc aataaattac tcctctcttc gatcacgttc ctcgtctaaa 6360 ccatatcagc ttatttcatg acctttttta catgatcttc gattaacagc tttcttataa 6420 agtctcttaa cgtatatttt ttaatgctat ttatataaac ttttttactg aacaattaaa 6480 ttcaataatt tcttttaaaa ctgttgtaaa aaaattagat tatcggtgta tttatttata 6540 ttagtcaatc tttctatgac tattttcgtt gaagatggaa cgaatactat gagaagatca 6600 cggaaagaaa acaaaaagca atcctaaatg gaaagatc 6638 //