ID SPAC1F12 standard; DNA; FUN; 14399 BP. XX AC Z69944; XX SV Z69944.1 XX DT 07-MAR-1996 (Rel. 47, Created) DT 24-NOV-1999 (Rel. 61, Last updated, Version 3) XX DE S.pombe chromosome I cosmid c1F12. XX KW endonuclease; heme binding protein; phosphoserine aminotransferase; KW putative 5'-AMP-activated, gamma subunit family; SNF4 homologue; KW TCTP homologue; tumour protein homologue. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-14399 RA McLean J., Harris D.; RT ; RL Unpublished. XX RN [2] RP 1-14399 RA Barrell B.G., Rajandream M.A., Walsh S.V.; RT ; RL Submitted (05-MAR-1996) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Hinxton Hall, Hinxton, Cambridge CB10 1RQ E-mail: barrell@sanger.ac.uk XX DR GOA; Q10344; Q10344. DR GOA; Q10345; Q10345. DR GOA; Q10348; Q10348. DR GOA; Q10349; Q10349. DR GOA; Q10351; Q10351. DR GOA; Q10352; Q10352. DR SWISS-PROT; Q10343; YL28_SCHPO. DR SWISS-PROT; Q10344; TCTP_SCHPO. DR SWISS-PROT; Q10345; YDA3_SCHPO. DR SWISS-PROT; Q10346; YDA4_SCHPO. DR SWISS-PROT; Q10347; YDA5_SCHPO. DR SWISS-PROT; Q10348; YDA6_SCHPO. DR SWISS-PROT; Q10349; SERC_SCHPO. DR SWISS-PROT; Q10350; YDA8_SCHPO. DR SWISS-PROT; Q10351; PIGS_SCHPO. DR SWISS-PROT; Q10352; YDAA_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/yeast/home.html) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1F12 is not overlapped by any other sequenced cosmids. XX FH Key Location/Qualifiers FH FT source 1..14399 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1F12" FT /map="IR" FT misc_feature 1..99 FT /note="nominal overlap with cosmid SPAC1556, S. pombe FT chromosome 1" FT CDS join(complement(1007..1042),complement(820..943), FT complement(407..765),complement(203..359), FT complement(1..147)) FT /db_xref="SWISS-PROT:Q10343" FT /label=SPAC1F12.01c FT /note="SPAC1F12.01c, len:>273, SIMILARITY:Saccharomyces FT cerevisiae, SNF4_YEAST, nuclear protein snf4, (322 aa), FT fasta scores: opt: 917, E():0, (51.3% identity in 267 aa)" FT /partial FT /gene="SPAC1F12.01c" FT /product="putative 5'-AMP-activated, gamma subunit family" FT /gene="SPAC1556.08c" FT /protein_id="CAA93805.1" FT /translation="MTDVQETQKGALKEIQAFIRSRTSYDVLPTSFRLIVFDVTLFVKT FT SLSLLTLNNIVSAPLWDSEANKFAGLLTMADFVNVIKYYYQSSSFPEAIAEIDKFRLLG FT LREVERKIGAIPPETIYVHPMHSLMDACLAMSKSRARRIPLIDVDGETGSEMIVSVLTQ FT YRILKFISMNCKETAMLRVPLNQMTIGTWSNLATASMETKVYDVIKMLAEKNISAVPIV FT NSEGTLLNVYESVDVMHLIQDGDYSNLDLSVGEALLKRPANFDGVHTCRATD" FT misc_feature complement(148..159) FT /note="splice branch and acceptor sequence, ttaacctgagcag" FT misc_feature complement(197..202) FT /note="splice donor sequence, gtttgt" FT misc_feature complement(198..302) FT /note="Match to PF00571 CBS, CBS domain Score 23.11" FT misc_feature complement(360..375) FT /note="splice branch and acceptor sequence, ctaatttcaaattta FT g" FT misc_feature complement(401..406) FT /note="splice donor sequence, gttcgt" FT misc_feature complement(413..577) FT /note="Match to PF00571 CBS, CBS domain Score 31.36" FT misc_feature complement(766..780) FT /note="splice branch and acceptor sequence, ttaatcacgtcttag FT " FT misc_feature complement(814..819) FT /note="splice donor sequence, gtacga" FT misc_feature complement(944..963) FT /note="splice branch and acceptor sequence, ttaatctatgtgatg FT tacag" FT misc_feature complement(1001..1006) FT /note="splice donor sequence, gtaagt" FT CDS complement(1900..2406) FT /db_xref="GOA:Q10344" FT /db_xref="SWISS-PROT:Q10344" FT /label=SPAC1F12.02c FT /note="SPAC1F12.02c, len:167, SIMILARITY:Saccharomyces FT cerevisiae, TCTP_YEAST, translationally controlled tumor FT protein homolog, (167 aa), fasta scores: opt: 757, E():0, FT (64.3% identity in 168 aa)" FT /product="translationally controlled tumor protein homolog" FT /gene="SPAC1F12.02c" FT /protein_id="CAA93806.1" FT /translation="MLLYKDVISGDELVSDAYDLKEVDDIVYEADCQMVTVKQGGDVDI FT GANPSAEDAEENAEEGTETVNNLVYSFRLSPTSFDKKSYMSYIKGYMKAIKARLQESNP FT ERVPVFEKNAIGFVKKILANFKDYDFYIGESMDPDAMVVLMNYREDGITPYMIFFKDGL FT VSEKF" FT misc_feature complement(1912..2406) FT /note="Match to PF00838 TCTP, Translationally controlled FT tumor protein Score 409.21" FT misc_feature complement(1981..2019) FT /note="PS01003 Translationally controlled tumor protein sig FT nature 2" FT CDS join(complement(3011..3032),complement(2739..2830)) FT /db_xref="GOA:Q10345" FT /db_xref="SWISS-PROT:Q10345" FT /label=SPAC1F12.03c FT /note="SPAC1F12.03c, len: 37, splicing uncertain but FT contains 18 contiguous amino acids identical with SW:COX FT 2_SCHPO P21534 cytochrome c oxidase polypeptide II, (60.6% FT identity in 33 aa overlap)" FT /gene="SPAC1F12.03c" FT /product="very hypothetical protein" FT /protein_id="CAA93807.1" FT /translation="MYDIILLKINTHTVKYGSIVEFIWTLIPALILILHAS" FT misc_feature complement(2831..2847) FT /note="splice branch and acceptor sequence, ttgattaaagaacat FT ag" FT misc_feature complement(3005..3010) FT /note="splice donor sequence, gtatat" FT CDS complement(3438..4013) FT /db_xref="SWISS-PROT:Q10346" FT /label=SPAC1F12.04c FT /note="SPAC1F12.04c, len:191" FT /gene="SPAC1F12.04c" FT /product="hypothetical protein" FT /protein_id="CAA93808.1" FT /translation="MSYMHSLNLMLQNPSGIDKIAAILVNVARLDPASSKSTAQLVSML FT NEFRCILRLPGLYKLIVNFRKDSSPETYMSNAINIGYYVTEGLAFLGGKQIISISKPLE FT DKLWLWSSRFWLLDTLLTIYQLLREKTEDEKEHQLDLASNLASLPLCIHWSVENGAGLH FT KHQIGVLGLFSAIVQTRKLILSLHNKRA" FT CDS 5494..6627 FT /db_xref="SWISS-PROT:Q10347" FT /label=SPAC1F12.05 FT /note="SPAC1F12.05, len:376, SIMILARITY:Schizosaccharomyces FT pombe, O60150, hypothetical 40.1 kd protein., (361 aa), FT fasta scores: opt: 896, E():0, (38.2% identity in 364 aa)" FT /product="hypothetical protein" FT /gene="SPAC1F12.05" FT /protein_id="CAA93809.1" FT /translation="MPSLVRRFQHASTEEQPFELNIQMESPPLVMYGNPDVSSGALASG FT LAKLTVFPADLKCESIVMEFVRIISTKRPLRDSCPDCKAQVEAFEKWEFSKEPTVYTHG FT THTWPFTFIIPGHFPQTTNSPFINVTYILKTRVKIPSQPDFVFEYPLNLKRSIVTNADK FT IAQRLFPPTSLIALLELPPVIHPLSCVPVEFQLTGVKPENSKVGWRLTKISWRIEEQIK FT AQINGCSTHTGTKKPYIFKETRLLGNNEHKSGWKEDGDRIIFEIPISTSLLSKPICDVS FT FDGQFSLYIAHQLILETIVVEVMNSHPINSNARILRMKVNLPLTERGGLGVSWDEECPP FT MFNSVGPSPPAYEQVARSSPTDIPLPPPSCPTNVQRD" FT CDS join(complement(7399..7765),complement(6921..7312)) FT /db_xref="GOA:Q10348" FT /db_xref="SWISS-PROT:Q10348" FT /label=SPAC1F12.06c FT /note="SPAC1F12.06c, len:251, SIMILARITY:E coli, FT SW:NFI_ECOLI ENDONUCLEASE V, (223 aa), opt:226, FT E():1.3e-08, (28.6% identity in 227 aa overlap)" FT /product="putative endonuclease" FT /gene="SPAC1F12.06c" FT /protein_id="CAA93810.1" FT /translation="MNNDNLHDKLTIWRQQQIELKGMLQLIPKFQSLNEIRYVVGLDIS FT FDKSSPKAVSALVIYDLEQRMIIYKDYLCIEKLEEDYVPGFLSFREIKWYLPLLNHIPH FT QFRIDIILVDGNGVLHPVGFGLACHLGVLLNLPVVGVAKNYLHCVGLTESLDAHRETLK FT KHVLKKTTDHPILIHSIDQSSEILGAAVWTSSNSKRPVYVSIGNQMNLEQSIQLVQKCS FT SSHSRVPEPIRQADIYAKFVLSQSKRNKKN" FT misc_feature complement(7313..7326) FT /note="splice branch and acceptor sequence, ctaactcaacttag" FT misc_feature complement(7393..7398) FT /note="splice donor sequence, gtaagt" FT CDS 8225..9394 FT /db_xref="GOA:Q10349" FT /db_xref="SWISS-PROT:Q10349" FT /label=SPAC1F12.07 FT /note="SPAC1F12.07, len:388, SIMILARITY:Saccharomyces FT cerevisiae, SERC_YEAST, phosphoserine aminotransferase, FT (395 aa), fasta scores: opt: 1035, E():0, (41.6% identity FT in 394 aa)" FT /gene="SPAC1F12.07" FT /product="putative phosphoserine aminotransferase" FT /protein_id="CAA93811.1" FT /translation="MSSREEVVNFAAGPAAMITSVVEEFGKDFVNFQGLGMGVAEISHR FT SKQGSGIVTSAESNFRKLYNIPENFHILFMQGGGTEQFAACLYNVYAHHALKNGNAKSL FT VANYIITGAWSKKAYAEAERLGFPCHVAVDMKELAGKYGSLPEDKDLKFTPDGETSLVY FT YCDNETVHGVEFNEPPTNIPKGAIRVCDVSSNFISRKIDFTKHDIIFAGAQKNAGPAGI FT TVVFVRDSVLARPTPAELHKLNIPVSPTVSDYKIMADNHSLYNTLPVATLHAINLGLEY FT MLEHGGLVALEASSIEKSKLLYDTLDKHDLYISVVEPAARSRMNVTFRIEPQELESEFL FT AEAEKHHLVQLKGYRSVGGIRASLYNAISVEQTRRLIDLLESFAKAHSN" FT misc_feature 8273..9352 FT /note="Pfam match to entry PF00266 aminotran_5," FT misc_feature 8282..9340 FT /note="Match to PF00266 aminotran_5, Aminotransferases FT class-V Score 107.16" FT misc_feature 8846..8899 FT /note="PS00595 Aminotransferases class-V pyridoxal-phosphat FT e attachment site" FT CDS join(9836..10175,10223..10363,10407..10915) FT /db_xref="SWISS-PROT:Q10350" FT /label=SPAC1F12.08 FT /note="splice prediction is tentative" FT /note="SPAC1F12.08, len:328, SIMILARITY:Saccharomyces FT cerevisiae, YG5T_YEAST, hypothetical 34.3 kd protein, (305 FT aa), fasta scores: opt: 231, E():6.2e-08, (30.7% identity FT in 287 aa)" FT /gene="SPAC1F12.08" FT /product="hypothetical protein" FT /protein_id="CAA93812.1" FT /translation="MGKTLIFIKLSSLNECIKYSTIISKIARELSIHKGNELYIWVSCS FT EMCQKRTKHLFRELQNALCELYLSSATTNDSSLLSVVPTIVLFEYWSNIQLGSITDSWN FT SVYYSENAKDQILNIPAKSFQSIGPDVLNEIHNIEHLPKKDESEDSGKEINNMVSAVGG FT TFDHLHVGHKVLLTLTAWFGVKEVIVGVSGTKNENGRTLIILGDELLKKKVQKEFLENI FT QKRKEEVSNFLHSIKEDINCRVVTIHDPFGPTITDAEIDSLIVSEETKTGATAVQEERV FT KRGLPELSIYCIDLLYPAQELNLKDNNSLKVSSTAIREELAKLAYKNK" FT misc_feature 10176..10181 FT /note="splice donor sequence, gtaaat" FT misc_feature 10202..10222 FT /note="splice branch and acceptor sequence, ttaataactattttt FT ttgaag" FT misc_feature 10364..10369 FT /note="splice donor sequence, gtacgg" FT misc_feature 10390..10406 FT /note="splice branch and acceptor sequence, ctaattatttaatgt FT ag" FT CDS join(11040..11279,11326..12049,12092..12792) FT /db_xref="GOA:Q10351" FT /db_xref="SWISS-PROT:Q10351" FT /label=SPAC1F12.09 FT /note="SPAC1F12.09, len:553, SIMILARITY:Saccharomyces FT cerevisiae, Q04080, d9461.20p., (534 aa), fasta scores: FT opt: 247, E():6.6e-09, (23.9% identity in 510 aa)" FT /gene="SPAC1F12.09" FT /product="hypothetical protein" FT /protein_id="CAA93813.1" FT /translation="MSPCKWLTMFLKGCWGKIANMNHLDSCFPLRYIIGRKKASKLLCT FT MDSGLKSDSHEVERSNFQLNKPEKSLKRYALLSFYVIILLAIPVWWKTTHYERSSLPFE FT DMENAPSTVQTHLRFSPTFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVLEDDPVDYR FT IVLRESTDLQWFWDENNFIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSST FT VEPSRAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKL FT NIETQIQYFVEDAEAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPS FT PQIQPLWLENEDSNIIPTNSMLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLL FT LLLGINDVPVSSLSATLADRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMY FT VKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYA FT PLFAPILIPLLISFIKEVKDMLRERKLHRVANVPKPN" FT misc_feature 11280..11285 FT /note="splice donor sequence, gtgcgt" FT misc_feature 11311..11325 FT /note="splice branch and acceptor sequence, ctgacatagaagtag FT " FT misc_feature 12050..12055 FT /note="splice donor sequence, gtatgt" FT misc_feature 12078..12091 FT /note="splice branch and acceptor sequence, ctaaccctaataag" FT CDS join(complement(13560..13757),complement(13228..13405), FT complement(13099..13166)) FT /db_xref="GOA:Q10352" FT /db_xref="SWISS-PROT:Q10352" FT /label=SPAC1F12.10c FT /note="SPAC1F12.10c, len:146, FT SIMILARITY:Schizosaccharomyces pombe, O74875, hypothetical, FT (145 aa), fasta scores: opt: 537, E():1.8e-29, (55.1% FT identity in 147 aa)" FT /product="hypothetical heme binding protein" FT /gene="SPAC1F12.10" FT /protein_id="CAA93814.1" FT /translation="MVALFKSSLGRPEQHQTPQIRISPASSNVEHSEKQPRRDFRVKKY FT VAPGFSQLNWSKLVASGQNLSGVEKPIPVTKEELAKHKTKEDCWIAIRGKVYNVSAYLP FT YHPAGQKRILDYAGRDATVIFMKFHAWVNEEALLKTSFVGFLV" FT misc_feature complement(13167..13183) FT /note="splice branch and acceptor sequence, ctaacaattgaattt FT ag" FT misc_feature complement(13222..13227) FT /note="splice donor sequence, gtaagt" FT misc_feature complement(13228..13384) FT /note="Match to PF00173 heme_1, Heme-binding domain in FT cytochrome b5 and oxidoreductases Score 38.14" FT misc_feature complement(13406..13425) FT /note="splice branch and acceptor sequence, ctaacgaactgttga FT ag" FT misc_feature complement(13554..13559) FT /note="splice donor sequence, gtaagt" FT misc_feature complement(14300..14399) FT /note="nominal overlap with cosmid SPAC4H3, EMBL:Z69380 FT S.pombe chromosome 1" XX SQ Sequence 14399 BP; 4656 A; 2581 C; 2511 G; 4651 T; 0 other; gatcagtagc tcgacaagtg tggacgccat caaagttagc tggtcttttc aataaagcct 60 cgcctacgct caagtctaaa ttactataat caccatcttg tataagatgc attacatcca 120 ccgattcata gacatttagc agggtacctg ctcagttaat atataaatta ttcacagaaa 180 aaattcaaaa catctcacaa accttcagaa ttaacgattg ggacggcaga tatattcttt 240 tcggcaagca ttttaataac atcatagact ttcgtttcca tagacgcggt agctaaattt 300 gaccatgtcc caattgtcat ttggtttaat ggtacccgta gcattgcggt ttctttgcac 360 taaatttgaa attagtaaat ataaaaacat aacacgattt acgaacattc atggagataa 420 atttcaaaat tcgatattgt gtcaaaacag atacaatcat ttcgctgcct gtctcgccat 480 ctacgtcaat taaaggaatt cttctagcac ggctctttga catggctaag caagcatcca 540 taagactatg cattggatga acatatatag tttccggagg aatggcgcca atctttcttt 600 ccacttcacg aagtcccaac aatcgaaatt tgtcaatctc agctatagcc tcgggaaatg 660 aagagctttg gtaataatac ttaataacat tgacgaaatc cgccatcgtt aaaaggcccg 720 caaacttatt cgcttcactg tcccataaag gagcactgac gatatctaag acgtgattaa 780 taagttgatt tcacatctgt aatgaaaaat ctttcgtact gtttaatgta agaagagaaa 840 gtgatgtttt tacaaaaagc gtaacatcga acacgattaa acggaaggac gtaggcaaaa 900 catcgtaact tgtacgcgag cgtataaatg cttgaatttc tttctgtaca tcacatagat 960 taataattat ttctatttaa taaaaaatct attccatatc acttacgagt gctccttttt 1020 gggtctcttg aacgtctgtc atgacctcgt caaaggatat atggtgtggt tggtaaaaga 1080 gactagataa atgagcgagg ggaatggtaa cccgcacaag ggcaacaaat cgaggtgtta 1140 agtaattagt tgcaaatagt acaatgccta aattatgcgt atttgttagt gcttttaaat 1200 gatatatttc acttagggga atattgtagc taaactcgtt gatttaaact gcttatatag 1260 tttactattt aaacgtaaca atatacattg acaaaattaa atgcaactag tctattaact 1320 caatgataag attgattaaa aggcttaaca attaagcata aatttaatcc aatagtcgtc 1380 cgctacaaaa ataacatcgt cgagcaaaaa tttcccaaat ctttagtact aacagtaaac 1440 tatagtttcg actgtaacaa agtttcaatg gctaccgggt cgtctagtgt cgccataaca 1500 aaagtttggc tattcctcgt aaaggacttg aattatcaac ttcgataaaa agcaaaaaaa 1560 tacactaaat taatggtaaa ataccaaatt tcaaaccata tccttactta ctaaattatt 1620 tcggcctgtt aatatataaa tagattcatg aattttagca cacacgtttt aaaacatcga 1680 tacctcaatc ttccattcaa ccaaatacaa aatcgataaa aaaacactaa tttcaatttg 1740 aacatgatta taagctagca taattaatta ttatcagaac aaaaacccct gttctaatcc 1800 taatgaaatt ttgtgaaaac taattcacaa aactataaaa tttagacatg atatcatgtt 1860 cccagttaga aactccctta taagaaccca aatttgcatt tagaacttct cagaaacaag 1920 accgtccttg aagaagatca tgtagggagt aataccatcc tcacggtagt tcattaagac 1980 aaccattgcg tcgggatcca tgctctcacc aatgtaaaag tcgtagtcct tgaagttagc 2040 caaaatcttc ttgacgaatc caatggcatt cttctcgaaa acaggaacac gctcggggtt 2100 gctttcttga agacgagcct tgatagcctt catgtaaccc ttgatgtaag acatatagga 2160 cttcttgtca aaagaagtag ggcttaaacg gaaagagtaa acaagattgt ttacagtctc 2220 agtaccctct tcagcattct cctcagcatc ctcggcagaa gggttggcac caatgtcaac 2280 atcaccacct tgcttgacag tgaccatttg gcaatcagcc tcgtagacaa tatcgtctac 2340 ctccttcaag tcgtaggcgt cagaaacgag ttcgtctcca gaaataacgt ccttgtacaa 2400 caacatggtg attgcagatg gtagctcata atttgacttc tcgcaggttt aatgagataa 2460 agcagtcaca gtttagggca gggttactgg attctagcca tccgtatgga atgcggttac 2520 gaacagtatt cactttacgt agtacagcgt tgttttcgat agatacattt tgtttaccaa 2580 ttccttcatt tttttcaatt tgtcgtagtc tgttctcctt tcttttaaat aacaatattc 2640 tttatatttt cagatttttt tttagatgaa aatatcgttg tactcagtca gtagtaaatt 2700 gttttatatt actgaatctg attttttgag tgaaaccatt atgatgcgtg taaaattaag 2760 attaaagctg gaattaaagt ccaaatgaat tcaacaattg aaccatattt aacagtatgt 2820 gtattaatct ctatgttctt taatcaactt gtaaccattt atgtcgcctc aaatttatta 2880 ttcgagctat tatccgtcaa ttcttgagtc gtaattagaa cattctcaac agtactgagc 2940 ttcagaagaa acctaaaaaa gtagtgcatt atagttgata ataattcctt aacgtttata 3000 tatgatatac ttaaaagtat gatatcgtac atcctcaatt tgaaattgca gggccattaa 3060 aaacactcgt taataaagat attcagttca attagaaaaa aatgacaagg tacattttgg 3120 tgacaaacaa agagtaactt aatgttttcc gttgattaaa ttttcgatca atatcccaaa 3180 atttaaaacg ctttaatgga atggattaac gattaaaatc gacttcattt ctaaaacctt 3240 tcaagcttta aaccgattca gtcccttcat ttagaatggc atatttcgat ttttgctgaa 3300 aaaaccaaga cgactgttac cacttttgtt tactacttta ctcacataga cattcgagaa 3360 aacgaaaaat agttgagcac gaatagaatt acatgaaaaa agtaacttct atttaaattt 3420 aaaaagctca ttcgtaacta agctctttta ttgtgcaaag agaggattag ttttctagtt 3480 tgtacaattg cgctgaaaag accaagaact ccaatttggt gtttgtgtaa accagctcca 3540 ttttctacag accagtgaat acacaaagga aggctagcca agtttgaagc taagtctaac 3600 tgatgctcct tttcgtcttc tgttttttca cgcaaaagct ggtaaatggt caataaagta 3660 tcgagaagcc aaaatctaga agaccaaagc cataatttgt cttccaacgg ttttgatatg 3720 gaaataatct gtttgccgcc taagaatgcc aatccttctg tgacatagta tccaatattg 3780 attgcgttac tcatgtaagt ttcaggagaa gaatctttac gaaaatttac gattaatttg 3840 tagagaccag gaagacgtaa aatacatcga aattcattta gcatagaaac cagttgagca 3900 gttgatttcg aagatgctgg atccaacctc gcaacgttga caagaatggc agcaattttg 3960 tcaattccgc tgggattttg cagcatcagg tttaaagaat gcatataaga catatttctc 4020 aaaaaatttc agctctaaga tttggtcaat tatattgttc acgaaaggta gtctaatttt 4080 cgtatacttt agtagaaaag cgtaaaatcc aaaaaaatac taagtagcgt atgtaaacga 4140 ctaaaattac caaatccaaa gattatttga atgacgatag atgtcggttc ggcaacgtct 4200 tatggtatag agagtgagtg tgctgctata gtcgatcgga tcagcaaacg ttagctagtc 4260 tgtttagtac accaatagat ttgataagaa aaatctacag tgcagcaaag tgaaatgtta 4320 ttttatgagt tgatcgattt tacttttaga attacaaatg ataagatatg ataactttct 4380 ccagttatca cgtcgattag atcgccggaa ataactccga tattgaggaa agtgtattca 4440 gttgcaacaa acagctgtga cggcttcaaa gtatcggcaa atcatttttt gcatgtgttt 4500 taaaaaaata gttcagcaat aaaaaacata aatttgttag gcttctagag gcatatgctt 4560 atttatgtgt tattttttta atatttatca taacgtattt tttattacaa atccttaggt 4620 agcaatatct taagaattaa acatacatat gttcaaggat aaaagaaaga aaaacttgga 4680 aactgggttt attttttcta ttcctttgtt cagatacata taaaacagct tagttactat 4740 ggattattat catatagaat tatgataact gtttaaaata aatattattt ggaataacga 4800 gatttgttta ggtcggcttg agatacgcac agctacgtaa gattgtagat aacaaacttt 4860 gaaaaccttc gcattcagtg gcctggggag catgcagact acacttccta ttcttccttt 4920 gcaaaaacct tctcttctta ctacattagt atatttttca tagggaggtg ctatatgcac 4980 tgtattcata gttacgctct gcttacagtg agcaaagtat ttctttggac gctaaaaaaa 5040 ggctcctgta tattgatttg agtaaatatt ttggcataga aaaacacatt caacgctact 5100 taattgtttt ttccatttcg aactactatt gagaggaagt tgttttttca attgttttat 5160 atcatttgaa aaaaattgaa gcacaattgc ttatctttga tcaaacgttt aaggagtaag 5220 ctccaaattc gataatccct attccgttct acataaaacc tgagttcatc tcttgtgctg 5280 tcgctttcaa cctcagaagc tgttaaggat atatattaat ttaaaatttt taccaaaaaa 5340 tatatttcat tctcttcata gccgttttct gtttgtttaa tttcaatctc atattaattc 5400 ttctgatcgg gccccccttt gttctttaga ttcatttttt cctactcttt ccttttaata 5460 tttaattcct aagtcattag ctgaaccgtc aagatgcctt cattagtcag gagatttcag 5520 catgcttcaa cagaagaaca acctttcgaa ctcaacatcc agatggagtc tcctcctttg 5580 gtaatgtatg ggaatccaga tgtttctagt ggagcgttag catctggact tgccaagttg 5640 actgtatttc ctgcagatct caaatgcgaa tccattgtca tggaattcgt ccgcataata 5700 tccacaaagc gtcctttgcg cgacagctgt cccgattgca aagctcaggt tgaggctttt 5760 gagaaatggg agttttctaa agaacctacc gtatatactc atggtacaca tacttggcca 5820 ttcactttta taatacctgg acattttcca caaacgacca actcaccatt cataaacgtt 5880 acttacatcc taaaaactcg cgttaagatt cccagtcaac ccgattttgt ttttgaatat 5940 cctttaaact tgaaacgctc tattgtcact aatgctgata aaattgccca gcgtctcttc 6000 ccccctactt cgcttattgc tcttttagag ctgcctcctg taattcaccc tttgagttgt 6060 gttcctgttg aatttcagct gaccggtgta aaacctgaaa actccaaggt tggttggcgc 6120 ttaacgaaga tttcgtggag aatagaagaa cagataaaag ctcaaataaa tggttgctcg 6180 acccatactg ggacaaaaaa gccatacatt ttcaaggaga ctcggctgtt gggaaataat 6240 gaacataagt ctggttggaa agaagatggc gatagaatta tcttcgagat ccctatcagt 6300 acatctttac tttcaaagcc tatttgtgat gtttcctttg atggacaatt ctctctatat 6360 attgcccatc aactaattct tgagactata gtcgtcgaag tgatgaactc gcatcccata 6420 aactcgaatg cccgaatttt aaggatgaag gtcaatttac ctttgactga acgaggtggt 6480 cttggtgtta gttgggatga ggaatgcccc ccaatgttca atagtgttgg acccagtcct 6540 cctgcatatg agcaagttgc tagatcctct cccactgata ttccactacc tccaccttca 6600 tgtcctacaa atgttcaaag agattgattc gaattttagt ttcttccgtc aattttgttt 6660 ttttatcttt ctgttttaat tgggcttgtt ttttcatttc aaagatttgt gtatctttat 6720 atgacttgaa cggtaatttt ttgaccttta ctaagcttgg atgatacaac aggatttcca 6780 ttaaaggttg cttaacgttt gttcaaattc cgctttttta tttctctttc ctgtagatta 6840 atctaattct aaaccgtcaa ttaaaatact cacagaatat ctaagtagac aaagtaatgt 6900 ttctcgtaag atgattttgc ttaatttttc ttattccttt tcgattgact taggacaaat 6960 ttggcataaa tgtctgcttg cctgatcggt tccggtaccc tgctatgaga gctactgcat 7020 ttctgtacga gttggataga ctgttctaaa ttcatttggt taccgatgct aacataaact 7080 gggcgtttcg agttactgct tgtccatact gcagcgccca gaatctcaga agattgatca 7140 atcgagtgaa tcaatattgg atgatcagtt gttttcttta gcacgtgttt ctttaaagtt 7200 tcacgatgtg catctaagct ttcagtcaaa cccacgcaat gtaagtaatt tttcgcgacg 7260 ccaacaacgg gaagattcaa caacacacct aaatggcaag ccaatccaaa tcctaagttg 7320 agttagatat aattaataat atccttcatt gagagtgtct ccttgaagta aatagattgt 7380 ttacagaatc ttacttaccg acaggatgca atacgccatt tccatctact aaaatgatat 7440 caattcgaaa ttgatgagga atgtgattta gcaatggcaa ataccattta atttctcgaa 7500 atgaaagaaa cccgggaaca tagtcctctt ctaatttctc aatgcacaaa taatctttat 7560 aaataatcat acgttgttct aaatcataaa taacaagggc tgagacagcc tttggacttg 7620 atttatcaaa agagatatcc aatcctacaa catatcttat ttcattcaaa ctttgaaatt 7680 tgggaattag ctgcaacatc cctttaagtt caatttgctg ttggcgccat atcgttaact 7740 tatcatgcaa attgtcatta ttcataatat acaattgatc ttaaaatcat ttaggtgttt 7800 tttaatttaa aataaaccgg tcttttctgg ggcttgagtt gagtacgaaa accacgaagt 7860 gaaaaggtag aagttcccac atatgagatt tagacgcgaa atgtcaatta atgtaatgag 7920 atatctaaaa tataaattac tgtaaaagaa ttaaattgac aaagcgtaaa cgaatacgct 7980 atcatttttg ggatctcatc ggtgtgtata aaaaagaagt agtgacttca ctcacttcct 8040 cttaacccat atcataggag ttgataattg gatcacgttg acaggttttg atttctctag 8100 gcgacattta ctattgtaat tagttttact ctcatataaa tagttttgga gtaatacctg 8160 tgaaaattga tattcagatt attttaaatt ctaagtatac ttctgcttga gctcacggaa 8220 caaaatgtca tccagagagg aagtagttaa ttttgctgcc ggtcctgccg ctatgattac 8280 ctctgtagtt gaggaatttg gaaaggactt tgtcaatttc cagggccttg gcatgggtgt 8340 cgctgaaatt tcccatcgca gcaagcaagg ttccgggatc gttacttctg ctgaatccaa 8400 ttttcgtaaa ctttacaata tccccgaaaa ctttcatatt ttatttatgc aaggcggtgg 8460 tacagaacaa tttgccgctt gtctttataa tgtttatgca caccatgccc tcaagaatgg 8520 caatgctaaa tctcttgttg caaactatat tattaccgga gcctggagta aaaaagcata 8580 tgctgaagct gaacgcttag gcttcccatg ccacgttgca gtcgatatga aagaattggc 8640 cgggaaatat ggatctttac cagaggacaa agatctcaaa tttacacctg atggcgaaac 8700 ttccttggtt tattattgtg acaatgaaac tgttcatgga gtcgaattta atgaaccacc 8760 tacaaacatc ccaaaaggtg ctattcgcgt ttgcgatgtt tcgtctaatt tcatttcaag 8820 aaaaatcgat ttcaccaagc atgacataat ttttgctggt gcacaaaaga atgctggtcc 8880 tgcaggaata actgttgtat tcgtcagaga ctcagttctt gcgcgtccta cccccgccga 8940 acttcataag ctcaatatcc ctgtctcccc tactgtttcg gattataaga ttatggctga 9000 taatcatagc ctttacaata cgcttccggt agccacgctc catgcaatta accttggctt 9060 ggaatatatg cttgagcatg gtggtcttgt tgctctcgaa gcatcatcaa tcgaaaagtc 9120 aaagttattg tacgacactt tggataagca tgatctttat atatctgtgg ttgaacctgc 9180 cgctcgtagt cgcatgaatg tcacattccg aattgaacct caggagcttg agtctgaatt 9240 tttagcagaa gccgaaaaac atcatcttgt gcaattgaaa ggttatcgca gtgtaggggg 9300 aattcgtgct tctctgtaca atgcgatttc cgtagagcag acccgtaggc ttattgatct 9360 tttagagtcc tttgctaaag cacactctaa ctaacctttt ttgattctgt cttaagtttt 9420 tgatttagat ataaaacaca atagatgtgg gagctgttgg tcctaagtat catatttatg 9480 aggatagaaa tgtattaacg taaaatctgt attaacgaaa gtcagtttca aaatcaccgc 9540 ttaaaagctt cattaacgta ttcagtaatc aaagctgcag cttgaaaagt aaatttggtg 9600 atttgaggat atttacgagt tttaagacat tctgggcttc atgtaagttt caacaacaat 9660 atcagcttct aagacagatt atatgtacgc agaataccta accaacaatt attacaagaa 9720 caaattttat tcataagaag ttgattgaaa ttattttttg gaagtcgtaa cattatcgga 9780 ttgtcccacc tgataccatt tcaaactaaa tataaaactt ttcaagagac gaataatggg 9840 gaaaactctt atatttatta agctaagcag cttaaatgag tgtattaaat attctacgat 9900 aatatcgaaa attgcgagag agcttagcat tcataaaggc aatgaattat atatctgggt 9960 aagttgctct gaaatgtgtc agaaaagaac gaagcatttg tttcgtgagt tgcaaaatgc 10020 gctttgtgaa ttatatcttt cttcagcaac tacaaatgat tcatctttgc tttctgttgt 10080 gcccacaatt gtattatttg agtattggtc gaacatccaa ttgggcagta ttacagattc 10140 atggaactct gtatattatt ctgaaaatgc taaaggtaaa ttgaataaca gaaagtattt 10200 attaataact atttttttga agaccaaatt ttgaatatac cggcaaagag tttccaatcg 10260 attgggccag acgtgctaaa tgagatccat aatattgagc atttacctaa aaaagatgag 10320 tctgaggatt ctggaaaaga gatcaacaat atggtctctg ctggtacgga ctcttaattc 10380 atttgtcaac taattattta atgtagtagg tggaactttc gatcatttac atgtcggcca 10440 taaggtcttg ctaactttaa ctgcctggtt tggagtaaaa gaggtgattg ttggtgtttc 10500 aggtacgaaa aatgaaaacg gtcgaacgct gataatttta ggagatgagt tgcttaaaaa 10560 gaaggttcaa aaggagtttt tagagaacat tcaaaagaga aaggaggaag tatcgaattt 10620 ccttcattct ataaaagaag atatcaactg tcgtgtagtc actattcatg atccgtttgg 10680 acctaccata acagatgccg agattgacag tttgattgtt tctgaagaga cgaagacagg 10740 cgcaacagct gtgcaagagg aaagagtaaa aagggggtta ccagaattat caatttactg 10800 tatcgacttg ttgtatccag ctcaagaact gaaccttaaa gacaataatt ccttaaaggt 10860 cagttcaacg gcaattcgtg aagaacttgc taaacttgct tataaaaata aatgacatct 10920 tttttttacg tttgagataa cttttgaact ggttttcagg ttgaaaactt ccaaattgaa 10980 tgtatgtaat ataaatcgta aatgtttaca attaaataat tatagtctct ctatatgaga 11040 tgtccccttg taagtggttg accatgtttc taaaaggatg ttggggtaaa atagctaaca 11100 tgaatcatct tgacagctgc tttccacttc gatatattat cgggcgaaaa aaagcctcca 11160 aattgttatg tacaatggat tctggtctca aatctgactc tcatgaggtt gagagaagta 11220 attttcagct taataaacct gaaaaatcac taaaacgata tgctttgcta tcattttatg 11280 tgcgtgctat acttatgata tttaaagttt ctgacataga agtaggtaat tattttatta 11340 gcaattccgg tttggtggaa aactacacat tatgaaagat caagtcttcc ttttgaagat 11400 atggaaaatg caccttccac tgtacaaacg catttgcggt tttctccaac ctttcgaata 11460 cttgatgata agggaaataa tttaactaag gaggtacaga aggtattaga agcagaaccg 11520 caaatttact catacaatct gaaagtgtta gaagatgatc cggtggatta tcgaatagtt 11580 ctacgcgaat caactgatct acaatggttc tgggatgaaa acaattttat aattgataca 11640 cctagcaaag gtccatcaga attggctatc ctaatcgtaa attgtttatg ggaggctttt 11700 agcccacagg taatggaggt atggagtaaa tttactcgtt tctcctctac agtggaacca 11760 tcccgagcag aaactaaacg tacagttcaa ttttctcctc aatatagagt actgttaagc 11820 ttattggtag gtgagggaaa tcatgaaccg ataaattggg atatcgagaa tgcgattcaa 11880 aaatacttta accccttgat cgaacaactt gcttcattag ctaaactgaa cattgaaacc 11940 caaattcagt atttcgtaga ggatgctgag gcgtatatta aagacgataa attttgcact 12000 aaacacgcgg atcttcccaa tttggttaat aactttgaaa aatatttgtg tatgtattga 12060 attatcgtac caacatacta accctaataa gcattctcgc cgcatatcag ggaacctaca 12120 attcattttg tactttatgt gcctagtcct caaatccagc ccctgtggtt agaaaatgaa 12180 gattctaata tcataccaac aaattcaatg ttactgcctc aatgggggtc catcactact 12240 attaatttta acgttactga gaagaaattg ctacatgatg ttgatcttaa agattacttc 12300 cgcgtgatct cgagagacct tctattgtta ttggggataa atgatgttcc agtgagctct 12360 ttgtcagcaa cattagctga tagactctta agacaaagaa tcgcggaaag ctgtatagag 12420 gctagtgaca cattgcaaaa tcttgccaag ctggttcatt ctatgcaaag tatggccgtt 12480 ccaaaggaaa ttcaaatgta tgttaaagat acactgctat cacttgatat ggcctataaa 12540 gctctttcgc aaaacaatct taatgaagca ttatcgtata gtaataatgc attttcaaaa 12600 tcgcaagagg cactttttca cccgagtatg gttacgacca tttatttccc cgatgaatct 12660 aaatatggga tttacgcccc tttgtttgct ccgattttaa ttcccttact tatatccttt 12720 atcaaagaag taaaggatat gttaagagaa cgtaaattgc atcgtgtagc aaacgtgccg 12780 aagccaaact aaccgatacc attattgaaa ttacaccgcg tatctaatat tttagaaatt 12840 attctaatga aaataattaa tgaatgaaat gtatgtagat tctgtgatat ttgtgttgat 12900 gcgaaaatgt gatgattaaa ggacatggca acgtctaaaa tcgggtaaaa gaaaagagaa 12960 tggcaataat tttttcatta tctaaatatt aagctctaaa atactacttt cccaatgtca 13020 catagatgta tgcaacccca gccaagacaa ctctttcacg taaaagcaaa agaaaaaaaa 13080 cataggaaaa ctctacagct acaccaaaaa cccgacaaat gaagtcttta ataatgcttc 13140 ttcattaacc caagcatgaa atttcactaa attcaattgt tagagatctg atgcctatga 13200 actttaaaaa atcacacaat cacttactaa atataactgt agcatctctc ccggcgtaat 13260 caaggattct cttctggcca gccgggtggt aagggagata agctgaaaca ttgtagacct 13320 ttccacgaat tgcaatccaa caatcctcct tggttttgtg tttagcaagt tcttctttgg 13380 tgacaggtat gggtttttcc actcccttca acagttcgtt agttaggtct ttaaagaaag 13440 ctcagatagt aaaatgaaag atgatgatga gtgatctttg gcaatttcga atttcgaaag 13500 ccaaatgctc aaaagccgac ttaaacaaaa caaacacatc attccaaaaa tttacttacc 13560 gataaatttt ggccactggc aacgagttta gaccaattta attgggaaaa tccaggtgcc 13620 acatattttt taactctaaa gtccctcctt ggttgctttt ctgaatgttc aacattgctc 13680 gacgcaggac ttatccggat ttgtggagtt tggtgttgct ctggtcttcc taaactactc 13740 ttaaaaagtg caaccattat tactcagtat tattatgagg caacccgaaa aatgccctca 13800 aagatttctt actcctatat gtagttggca aaagttaagt tcacaattct caatagcttt 13860 tcttcttata tagtagctga atggatttga tttcgtaagc gaacgctgct cataggtata 13920 tattttaatg ttcgttcaat aagcgacgct tgttctaagt gatcggcagt ttatgacgtg 13980 ttatccgtcg atcatttttc tagaataact agacgtttga tgagtcaaat tctgtttgca 14040 aataaataat aattataaaa cattataatt tcgtgttatt tccttcactt tatgtaattt 14100 aagaggaaaa aacttcacac gttggcttca aggttattag tataacaata ctacgatgca 14160 aacaagcgtt tatccggttt caatgctttt aatttagaaa aataaaagag atacttttta 14220 attacattta tctttaaaaa aacttttttt tttcaaggta cttgagtatt ttgatcaaac 14280 tttataagaa gccaagaatg atctgcgagt ggaaagaaaa gctgaagtgg gtgaaaaaga 14340 agatttttaa attctgaaat ttgatgaata cgactgtttt tagatttttc aaatctatt 14399 //