ID SPAC1B9 standard; DNA; FUN; 5997 BP. XX AC AL109951; XX SV AL109951.1 XX DT 17-AUG-1999 (Rel. 60, Created) DT 17-FEB-2003 (Rel. 74, Last updated, Version 4) XX DE S.pombe chromosome I cosmid c1B9. XX KW cAMP-dependent protein kinase; mating; KW nuclear transport factor, NTF2 homolog; sck1; similarity to yeast SSF1. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-5997 RA McDougall R.C., Rajandream M.A., Barrell B.G., Whitehead S., Churcher C.M.; RT ; RL Submitted (19-AUG-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O14206; O14206. DR GOA; P50530; P50530. DR GOA; Q10100; Q10100. DR SWISS-PROT; O14206; YDD3_SCHPO. DR SWISS-PROT; P50530; SCK1_SCHPO. DR SWISS-PROT; Q10100; NTF2_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1B9 is overlapped at the 5' end by cosmid c15F9 and at CC the 3' end by cosmid c6B12. XX FH Key Location/Qualifiers FH FT source 1..5997 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1B9" FT /map="IL" FT misc_feature 1..314 FT /note="nominal overlap with cosmid SPAC15F9 EM:Z68157 S. FT pombe chromosome 1" FT CDS join(complement(700..706),complement(575..657), FT complement(434..505),complement(280..381), FT complement(94..201)) FT /db_xref="GOA:Q10100" FT /db_xref="SWISS-PROT:Q10100" FT /gene="SPAC1B9.01c" FT /product="nuclear transport factor; similar to S. FT cerevisiae NTF2" FT /protein_id="CAB53052.1" FT /translation="MADYNALATQFTQFYYQTFDSDRSQLSSLYREESMLSFEGAQLQG FT TKAIVEKLVSLPFQRVQHRISTLDAQPTGTTGSVIVMVTGELLLDEEQMAQRYSQVFHL FT VNNNGNYYVLNDLFRLNYG" FT misc_feature complement(202..216) FT /note="ttaacctttttgtag, splice branch and acceptor" FT intron complement(202..279) FT /note="confirmed intron" FT misc_feature complement(274..279) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(382..398) FT /note="ttaactgtttggtatag, splice branch and acceptor" FT intron complement(382..433) FT /note="confirmed intron" FT misc_feature complement(428..433) FT /note="gtatga, splice donor sequence" FT misc_feature complement(506..523) FT /note="ctaacttatatatattag, splice branch and acceptor" FT intron complement(506..574) FT /note="confirmed intron" FT misc_feature complement(569..574) FT /note="gtatgc, splice donor sequence" FT misc_feature complement(658..671) FT /note="ctaatactctgcag, splice branch and acceptor" FT misc_feature complement(694..699) FT /note="gtaaga, splice donor sequence" FT CDS join(complement(3501..3740),complement(2694..3384), FT complement(1678..2644),complement(1435..1627)) FT /db_xref="GOA:P50530" FT /db_xref="SWISS-PROT:P50530" FT /gene="sck1" FT /gene="SPAC1B9.02c" FT /EC_number="2.7.1.37" FT /product="serine/threonine protein kinase; high copy number FT suppressor of defects in the cAMP-dependent protein kinase FT pathway (PMID 7498728); involved in trehalase activation FT (PMID 9245826); C2 domain; similar to S. cerevisiae SCH9" FT /protein_id="CAB53053.1" FT /translation="MTEIFGKLHRSSNSENTNQASPSTIQSHSTQPVLSNDHSTKVNDY FT EGKEGASSNGYDPVFMSDRMKMRYNEITAQLHKEQSLKEDKESGSNSSESNGITPMGTY FT SEKPKLLQSRTPPSSCYIRHDTVVPKDKNGQHAFGRLYVRLHQGRDLNVKSVNAQPYAV FT ITFEKTQVMVPPPFKDIDGGIPISIPSKNRPLAGSASGSSSGLHSELMLADVRCPHWDF FT ETVFDVTKMKSQMVVSVYDKYEDDKFLGSVKITPIFLHEYVQEAWYKLEPLDLTKSLEG FT EIKVETIYEHIEHVRYGPEDFTALRLIGKGTFGQVYLVRKNDTNRIYAMKKISKKLIVR FT KKEVTHTLGERNILVRTSLDESPFIVGLKFSFQTASDLYLITDYMSGGELFWHLQHEGR FT FPEQRAKFYIAELVLALEHLHKHDIIYRDLKPENILLDADGHIALCDFGLSKANLSANA FT TTNTFCGTTEYLAPEVLLEDKGYTKQVDFWSLGVLVFEMCCGWSPFYAPDVQQMYRNIA FT FGKVRFPKGVLSSEGRSFVRGLLNRNPNHRLGAVADTTELKEHPFFADINWDLLSKKKV FT QPPFKPNVQNDLDVSNFDKEFTNTNVKNINIVSNVDPANASTPLSNTIQDRFRGFTFVN FT KSIDEQFQNLGLQENEETDNLHACRTTTHSSVNSINSHGNPRTVDANDPVADTVFGETF FT EA" FT misc_feature complement(1628..1646) FT /note="ctaaccttaaactttatag, splice branch and acceptor" FT misc_feature complement(1672..1677) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(1779..1886) FT /note="Match to PF00433 pkinase_C, protein kinase C FT terminal domain Score 64.34" FT misc_feature complement(1887..2645) FT /note="Match to PF00069 pkinase, Eukaryotic protein kinase FT domain Score 265.75" FT misc_feature complement(2645..2658) FT /note="ctaactttatttag, splice branch and acceptor" FT misc_feature complement(2688..2693) FT /note="gtaaag, splice donor sequence" FT misc_feature complement(3385..3398) FT /note="ctaacctatcatag, splice branch and acceptor" FT misc_feature complement(3495..3500) FT /note="gtatgt, splice donor sequence" FT CDS join(5798..5821,5880..5939) FT /db_xref="GOA:O14206" FT /db_xref="SWISS-PROT:O14206" FT /partial FT /gene="SPAC6B12.01" FT /gene="SPAC1B9.03c" FT /protein_id="CAB53054.1" FT /translation="MGGIGKKRVKKRTHLKADPIQEAAIPKS" FT misc_feature 5822..5827 FT /note="gtatgt, splice donor sequence" FT misc_feature 5865..5879 FT /note="ctaatttaattatag, splice branch and acceptor" FT misc_feature 5907..5997 FT /note="nominal overlap with cosmid SPAC6B12 EM:Z98531 S. FT pombe chromosome 1" FT misc_feature 5940..5945 FT /note="gtatgt, splice donor sequence" FT misc_feature 5984..5997 FT /note="ctaattcttattta, splice branch and acceptor - missing FT AG in cosmid SPAC6B12" XX SQ Sequence 5997 BP; 2058 A; 995 C; 1041 G; 1903 T; 0 other; aatccggtaa tcgctacaaa agcataaaaa aaaagaaaat gaaattgcag ataatcaaga 60 tacttgtatt aaatattttc actatgatat tcttcaacca tagttcaaac gaaacaggtc 120 atttaagaca tagtagttac cattattgtt aacaaggtga aaaacttggc tatagcgctg 180 ggccatttgc tcttcatcca actacaaaaa ggttaaaaaa agagcaaagc aaaaaaaaaa 240 aaaatgaata gaataagtaa catcaatctt ataacttacc aaaagctcac ctgtaaccat 300 aacgattacg gatccagtag taccagtagg ttgagcatcc aaagttgaga tgcggtgttg 360 aacgcgttga aaaggcaggc tctataccaa acagttaata aaaaaaagga agacgtttca 420 gttattatca tacaacaagt ttttccacaa tggctttagt tccttggagt tgggctccct 480 caaaactcag catcgactct tctctctaat atatataagt tagctgccca gataaataca 540 cgtaggatta atttcataat tagaagtcgc atacgtatag tgaagaaagt tgagaacgat 600 cggaatcgaa agtttggtag taaaattgag tgaactgggt cgccaaagca ttatagtctg 660 cagagtatta gcaataaatc atacaaattt ttatcttacc agccataatg aaagatggtg 720 gtaaaaggta gacctattat ttcaagttcg aaaaatcgcg tcaaaaaaga tcgtacagac 780 accttaagta atttaacgta aaatgataca actcttaata tgtaaaataa catgataaac 840 ttatagttgt gttatttggc aaaagtcctc tattcgaata gtattggcaa tggtagaaaa 900 acgaatttgc aataagattt gaaggggttt tacttcatgc aaacaacagg atagatgttt 960 atccgttcct attgagacaa aatttatgag aaaaaatatg gtgctagcgt ctaactagta 1020 tcatgaacga aatggaacat aatatttact tgattgtgaa gattgcttat taaatgctac 1080 attctttttt ataacgatgt atttcacatt ttgcataatt aatgcacctt caatcataat 1140 taaaagaaag caacaagtat ttgagttggg ataaaaatgt aaaacttaat ctattaaatg 1200 ttcacataat aaaatatgaa gatgactaca aaatagaacg taacaaataa taaaggatag 1260 aaggaaacaa aagcaaaaca cccacagttt gctttaattc ctgggtaaac tagaaaacac 1320 ggagcgaagc ctttgagaga caaaataact ataacgaaag gggaacataa tacataataa 1380 gttatcttct attgtggata ataggagaca aattttttga ttggagcaag cttatcaagc 1440 ttcgaacgtc tcgccgaaca cagtatctgc gacgggatcg ttagcatcca ccgtacgtgg 1500 attcccatgt gaatttattg aattaaccga tgaatgggtt gttgtgcgac acgcatgtaa 1560 attatctgtc tcttcgtttt cttgtagtcc caggttctga aactgttcat caatggactt 1620 attgacacta taaagtttaa ggttagttga tggtcaaata tttaaaaata aacttacaaa 1680 gtgaaaccac gaaatctgtc ctgaatcgtg ttggatagag gtgtcgaggc attagcggga 1740 tcaacattgg aaactatatt gatatttttc acattagtat tggtaaactc tttatcaaaa 1800 ttggaaacat ccagatcatt ttgaacattc ggtttaaaag gcggctgaac cttcttcttg 1860 ctaagtaggt cccaatttat gtctgcaaaa aaaggatgct cttttaattc agtagtgtca 1920 gcaactgctc ctaaacgatg atttggattg cgatttaaaa ggcctcgaac aaagcttcga 1980 ccctctgatg aaagaactcc ttttggaaaa cggacctttc caaaggcaat attacgatac 2040 atttgttgga cgtctggagc ataaaagggg ctccatccgc agcacatctc aaagacaagg 2100 actcctaaag accagaaatc gacctgttta gtatacccct tgtcctcaag taaaacttca 2160 ggtgccaaat attcggtggt tccacaaaag gtattagtag ttgcgttagc tgaaaggttt 2220 gccttagaca aaccaaaatc gcaaagagca atatgaccat ctgcatctaa tagtatgttt 2280 tcaggcttta aatctcggta aataatatca tgtttatgca aatgttcaag agccaaaaca 2340 agttcggcta tataaaattt agctctttgc tcaggaaagc gaccttcatg ttgaagatgc 2400 cagaaaagtt cgcctccact catataatca gttatcaaat aaagatctga agcggtctga 2460 aaggaaaact ttaaaccaac aataaacgga gattcgtcta atgaagtcct gacaagtata 2520 ttcctttcgc ccaaggtatg tgtaacctct tttttacgaa caattaattt tttggatatc 2580 tttttcatag cataaatccg attggtatcg tttttacgga ccaaataaac ttgcccaaaa 2640 gtgcctaaat aaagttagta taccgtaaat tttttagctt aaagcagctt taccttttcc 2700 gattaaacga agggccgtaa aatcttctgg cccataacga acatgttcaa tgtgctcgta 2760 aattgtttcc acttttattt caccctccag agacttagtt aagtcaagag gctcaagctt 2820 ataccatgct tcctgcacat attcatgtag gaaaattggt gtaattttaa cggagcccaa 2880 aaatttatcg tcttcatatt tgtcatatac agatacaacc atttgacttt tcatttttgt 2940 cacatcaaat acagtttcaa aatcccaatg aggacaacgc acatcagcca acattagctc 3000 agaatgtagt ccagaagaag aaccggaagc agatccagca agaggacggt ttttagaagg 3060 aatggaaatg ggtatcccac cgtcaatgtc tttaaaagga ggaggtacca taacctgcgt 3120 tttttcaaag gtgattactg cataaggttg tgcattaaca cttttgacgt tcaaatctct 3180 accttgatgt aatcgaacat aaagccgtcc aaaagcatgc tgaccatttt tatcttttgg 3240 cacaacggta tcatgcctaa tgtaacacga agatggaggt gtacgactct gtagtagttt 3300 gggtttttct gaatatgtac ccattggtgt aattccattg ctttcggaac tattagaacc 3360 agattcttta tcttccttta aggactatga taggttagcc aagtgaacaa caatttatgg 3420 agacagtgat ttttgaaaaa cacaataaag aaagctatgg ggaagattgt ctttcaagca 3480 aaaaatcaat tataacatac ctgttctttg tgaagctgag ctgtaatctc attgtaacgc 3540 atcttcatcc gatccgacat gaaaacggga tcatagccat tagacgaagc accctctttc 3600 ccttcgtaat cgtttacttt cgtagaatgg tcattggaaa gaaccggttg cgttgaatgc 3660 gattgtattg tggatggcga tgcttgattt gtattctcag aatttgatga tcgatgcaat 3720 ttaccgaaaa tttcggtcat tttcagcaaa agcaatgatc aatcaagaat cttttaatac 3780 tcttttaatg gaatcggatt cctcgtctgg tgttactaat aatagggtgt ggtgtttaaa 3840 gttggttcga ccagcaggtg tttcgcatat gtaaccttcg caattccctt aatcgaaaag 3900 tgtggtaaca tccgggagta cgatatctta gcgaacggat agaaaatcca gtatttttcg 3960 aacactaatt cgtattttac tatgattata tagatgtata attttaaatg gttataatat 4020 cagaggggcg ccgcacttcc atttatctat ttcttaaggg attcaggtaa gggggatgta 4080 taaccctaaa catttgaaaa attcctttgc agtgtatttt attattatgc acttattcta 4140 aaagcagaaa aagaaattta tcgagcaatt aatacgctgt tgactcagaa atgtcttgtg 4200 attttcaaag gtttgtccaa cgaaggagta tcgaaacaga gttctccgaa tggcttagta 4260 gaaacttcat gtatatttat tttagcctac atatacaaaa gaaattaaag cacattacaa 4320 cgtaatttcg gagagctcaa gctatgagat tttcgactgt ttagtttcga ggaataccta 4380 gatagtaccg aaaaatgcta cactcttttt ttataaacat ttatcaaata atttttaatg 4440 cgttggctga tgccttaacc aaaggtcata ctaaatttac cagaaaagtt tctttttctt 4500 taataaatca attgtagtgt ttgaatgaat gagctttagt aggatgtcca ctggtcaatt 4560 aaaattgtaa cttactgaca tacattagca aatttgggca caatttctaa ttgttatttg 4620 cttctcatgg tttctgatat gcgtttgatg caaaatactc gggatttttg tatttcggct 4680 gatttaattt taattttaat ttttttttat ttaagtctct ctatactcat tactgtggtc 4740 aattatattc cttgagttta ttaaatcttt tatttacaga gtacaattaa ttaaaatgga 4800 gccaaaaaaa taacccttgt taaaaatttt tttaaactgt taattccctc cccaaaaaaa 4860 agatttaaaa ggaaaatgat taaaaaaaac gagaaataaa atttcatgta aaaagataac 4920 tttgtatagt aatttatata ataagagcta gtttgtcata gttcactgct attgtttatt 4980 attatttttt ttcatagcac agtatttcaa attaaatatc gtaaaagacc atcagaaatt 5040 acaaacaaaa taagaaaatc atcggttaaa ttaatgacat tcaaagtacc acttttcgtt 5100 cctctgacaa acgttgctac gcaaccttga ttagccgtac tagcaaaccc atcttagtac 5160 tttgtggggg ctttcatttt aataaccact gctaagctgc tagtgttagg cataaaaccg 5220 ataataagga ccatgtgggg gtcttccgca taggttaaac aaacagctgc tgaggcgagg 5280 ttcaggttat cgtggtgctt ttctacgtta atttatcaat acaaaatagt ttaatttcca 5340 aaacaaaagt caagagatat cagctacaat tacattaatg atatgcatca tccaaagtca 5400 gtgtacaaac aatagattta cgcccgacaa ataaaccggt atattttaga agttcatcat 5460 tttgtataca atcagttact tccaaaaaat tattgattaa ataagtgttt ataaaagatt 5520 tttcataatt tcgtagtacg gtcattattc tcaatttcac gtgttttttt ctttttttac 5580 ttctaggccc ataacattat tattaactat actagtataa aaaatttctc gctcaaatta 5640 tgttcacagc atgtaaatga gatatgttaa aatagaaaat tcagctttca aaaaaataat 5700 gtctattgcc atctttattg tcatgttggc ggaaatgaac tttgcatatt ggtgttcttg 5760 agagtgtaca atttttttta actaccaacc acaaagaatg ggaggcattg gtaaaaaacg 5820 ggtatgttga gaatggatag tttaaaagaa atactttatt taaactaatt taattatagg 5880 taaaaaagag gacgcatttg aaagctgatc ctatacaaga agcagcaatt cctaaatcgg 5940 tatgtgcaat tttgtaaaat tgtaaaattt tttagtccct ttgctaattc ttattta 5997 //