ID SPAC1851 standard; DNA; FUN; 5670 BP. XX AC AL133361; XX SV AL133361.1 XX DT 08-DEC-1999 (Rel. 62, Created) DT 08-DEC-1999 (Rel. 62, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c1851. XX KW 1-Acyl-sn-glycerol-3-phosphate acyltransferase; Acyltransferase; KW casein kinase II beta chain; Casein kinase II regulatory subunit; ckb1; KW confirmed intron; nuc2; nuclear scaffold-like protein p76; TPR Domain. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-5670 RA McDougall R.C., Rajandream M.A., Barrell B.G., Brown S., Harris D.; RT ; RL Submitted (03-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; P10505; P10505. DR GOA; P40232; P40232. DR GOA; Q9US20; Q9US20. DR SPTREMBL; Q9US20; Q9US20. DR SWISS-PROT; O42656; YLD4_SCHPO. DR SWISS-PROT; P10505; NUC2_SCHPO. DR SWISS-PROT; P40232; KC2B_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1851 is overlapped at the 5' end by cosmid c17C9, EMBL CC entry SPAC17C9, accession number Z73099 and at the 3' end by cosmid CC c27D7, EMBL entry SPAC27D7, accession number AL009227. XX FH Key Location/Qualifiers FH FT source 1..5670 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1851" FT /map="IR" FT misc_feature 1..126 FT /note="nominal overlap with cosmid SPAC17C9 S. pombe FT chromosome 1" FT CDS 1..452 FT /codon_start=3 FT /db_xref="GOA:P10505" FT /db_xref="SWISS-PROT:P10505" FT /label=nuc2 or spac17c9.01c FT /note="SPAC1851.01, len:149" FT /partial FT /gene="nuc2" FT /gene="SPAC17C9.01c" FT /gene="SPAC1851.01" FT /product="nuclear scaffold-like protein p76" FT /protein_id="CAB62427.1" FT /translation="QADFHFQRAAEINPNNSVLITCIGMIYERCKDYKKALDFYDRACK FT LDEKSSLARFKKAKVLILLHDHDKALVELEQLKAIAPDEANVHFLLGKIFKQMRKKNLA FT LKHFTIAWNLDGKATHIIKESIENLDIPEENLLTETGEIYRNLET" FT misc_feature 69..143 FT /note="Match to PF00515 TPR, TPR Domain Score 24.59" FT CDS join(806..1131,1195..1708) FT /db_xref="GOA:Q9US20" FT /db_xref="SPTREMBL:Q9US20" FT /label=SPAC1851.02 FT /note="SPAC1851.02, len:279, SIMILARITY:Emericella FT nidulans, Q9Y7C5, npgap., (291 aa), fasta scores: opt: 726, FT E():0, (47.2% identity in 254 aa)" FT /gene="SPAC1851.02" FT /product="putative 1-Acyl-sn-glycerol-3-phosphate FT acyltransferase" FT /protein_id="CAB62428.1" FT /translation="MGFIKSTLLATVTVFVGLCGINRFFTLPKCIRYHFRYFACHTFLA FT ISSAYGVIASVVARLCGYPVMGQYLTAKAYYGLASTILDFRFKIENEEILRKHKSAVLV FT VNHQSELDILAIGRTFGPNYSVIAKKSLRYVPILGWFMILSDVVFIDRSRRSDAIQLFA FT KAARRMRKENISIWVFAEGTRSYSLKPCLLPLKKGAFHLAVQAQVPIIPIAIQTYGHLF FT HPPTKVFNKGEALIKVLDPIPTEGKTAEDVNDLLHETETAMNNALVEIDDYGKVKKQ" FT misc_feature 1079..1615 FT /note="Pfam match to entry PF01553 Acyltransferase, FT Acyltransferase," FT misc_feature 1132..1137 FT /note="gtaagc, splice donor sequence" FT misc_feature 1174..1194 FT /note="ctgacttacaattttaattag, splice branch and acceptor" FT CDS join(2580..2602,2682..2961,3167..3559) FT /db_xref="GOA:P40232" FT /db_xref="SWISS-PROT:P40232" FT /label=ckb1 FT /note="SPAC1851.03, len:231" FT /gene="ckb1" FT /gene="SPAC1851.03" FT /product="casein kinase II beta chain" FT /protein_id="CAB62429.1" FT /translation="MQLYSSESESDDSQYWVDWFLGLKGNEFFCEVDEDFIQDRFNLTG FT LSHEVPHYSQSLDLILDVLDPDLPEEVQDEVEASARHLYGLIHARYILTAQGLYKMLEK FT YKKCDFGHCPRVLCNGQPMLPVGLSDIAHTKSVKLYCPRCEDVYTPKSQRHASIDGAYF FT GTSFPHMLFQVYPELAVPKSQERYIPRIFGFKVHSYSATFKKQDVYKEKQKKRLQGAEA FT ESKNKLAIT" FT misc_feature 2603..2608 FT /note="gtttgt, splice donor sequence" FT intron 2603..2681 FT /note="confirmed intron" FT misc_feature 2672..2681 FT /note="ttaattgcag, splice branch and acceptor" FT misc_feature join(2692..2961,3167..3424) FT /note="Match to PF01214 CK_II_beta, Casein kinase II FT regulatory subunit Score 458.53" FT misc_feature 2962..2967 FT /note="gtaagc, splice donor sequence" FT intron 2962..3166 FT /note="confirmed intron" FT misc_feature 3151..3166 FT /note="ctaacatcctttctag, splice branch and acceptor" FT misc_feature 3200..3295 FT /note="PS01101 Casein kinase II regulatory subunit FT signature" FT CDS complement(3855..5670) FT /codon_start=2 FT /db_xref="SWISS-PROT:O42656" FT /label=SPAC1851.04c FT /note="SPAC1851.04c, len:604" FT /partial FT /gene="SPAC1851.04c" FT /gene="SPAC27D7.01c" FT /product="hypothetical protein" FT /protein_id="CAB62430.1" FT /translation="STLQVCGAQTSDRLYISKSYEFCSSVKNNFGIDFWDQVEYPQPYV FT ASEWPIRYVSIKDDGSLIAIAGLHGLAIYVCSKKTWFLYKDANMEQLISVTCPMIWCSQ FT FLLAGVVCESNFELHLYKAKGPLDDRENLAKLSFTSTIVTMSVCDEYSLVVYTADNFLH FT HIRFDINELGRLELDYLTSVNFAPIFTTPSRVRSITLLLPKDLANIQPSDLLFYAVLLV FT LINGKLVLLSLKKQHSKELLYQCSMLAGDVEFYFINGSQEIPSLFHSIWIMTGKGLKLW FT LSFSEILSSVLINTKNLIDGLPKFLNTSKRVSLENFHRLSVEDLRSPSFVYKNGVDCNF FT DNTHLAKLIKNVNISEKFQKECIDLESQGCLLTVLSNYGLLLTAYTQGHKNLVNKMEYA FT HIQIGVYPFLPEIVRALLLLDKREQAIDLVKTYGHLHYINFVLEKLLSSSLLTYNSSQR FT DKLLYEVSLLFKDLQEFTTFKIVLGCLRKTEAEYWPMIFKYFGEPKDLMKKCLETEDVK FT SAAECLIIWQIHQGSASCASTFLSIYEMALKIKNWDVCTELSSYLVSLDPEKRLLKTAL FT ELLDEGPDSKDIRLYDQFNNLMKEVDKY" FT misc_feature 5567..5670 FT /note="nominal overlap with cosmid SPAC27D7 S. pombe FT chromosome 1" XX SQ Sequence 5670 BP; 1824 A; 988 C; 999 G; 1859 T; 0 other; atcaagcaga ctttcatttt caacgtgctg cagagatcaa tcctaacaac tctgtactca 60 tcacttgtat tggtatgatt tacgaacgct gcaaagatta caaaaaagca cttgattttt 120 atgatcgggc atgtaaactg gatgaaaagt cttcgcttgc caggttcaag aaagccaaag 180 tgcttatttt attacatgat cacgataaag cactcgttga attggaacaa ttaaaggcaa 240 ttgcgccaga tgaagcaaat gttcattttt tgctcggcaa aattttcaag caaatgcgga 300 aaaaaaattt agccttaaag cacttcacta tagcatggaa cttagacggc aaggctacgc 360 atattattaa ggaatcgatt gaaaatctgg atattcccga agaaaatttg ttaactgaaa 420 caggtgaaat ttataggaat ctggaaactt aagaaattat gtcttgtatt ttagggatgc 480 caaaaaattg tcgaatgaat aagttaatga gttagaatgt ttacgtcttc attctggtta 540 ggtttaaaaa tgatgatttt cgtttagaag ttatatatta aaataaaggt ataatagata 600 tcattaataa ccaatgataa aaaaaattgt gtataaaatc accgtgggta taacttaagt 660 ttaaagtgtg caattaatga aatgtcctca ggccattcat ttttattacg aatcaccaac 720 gttactatca ccgcaccaac catactttaa aacgaaagga agggttgttg aaagtttgct 780 cttctctact gtgtacatat tcgaaatggg ttttattaag agcacgctgc ttgctactgt 840 gacagtattt gttggtttgt gcggaatcaa ccgatttttt actctcccga aatgtattag 900 atatcacttt cgctatttcg catgtcacac atttttagct attagttctg cttatggggt 960 tatagcctca gttgttgcac gtttatgtgg ttatcccgta atgggacaat atttaactgc 1020 aaaggcatat tatggactgg ctagtacaat ccttgacttt cggtttaaaa tcgaaaacga 1080 ggaaattttg agaaaacata agtctgctgt cttagtagtt aatcatcaat cgtaagcata 1140 cctcaaaata tacttcttta tgataaaaaa taactgactt acaattttaa ttagtgaatt 1200 ggatattttg gctataggaa ggacttttgg ccctaattat tctgttattg ctaaaaagtc 1260 tctaagatat gtgcccattt tgggatggtt tatgatattg tctgacgttg tatttatcga 1320 taggtctcgc cgaagtgatg cgattcaact ttttgcgaaa gcggcacgaa gaatgcgcaa 1380 agaaaatatt tctatttggg tatttgcaga aggcactcgt tcctatagcc ttaagccctg 1440 tcttcttccc ttgaaaaagg gcgcatttca ccttgctgta caggctcaag tacccattat 1500 tcctattgca attcagactt atggtcattt gtttcatcct cctaccaaag tattcaataa 1560 aggtgaagct ctgattaaag tgcttgatcc aattcctacg gaggggaaaa ctgctgaaga 1620 tgtgaatgat cttctacacg agactgaaac cgctatgaac aatgcattgg ttgaaatcga 1680 tgactatgga aaggtaaaaa aacagtaaat aggaagaaga cacacaattg gtgttcttat 1740 ataattactt gttagttcaa ctgagagaag catttaccta tcctaaggtg gtttgtttcg 1800 ttttaaatca aataggtttg ataatatcat tgacgctttt ctttttttgt ttagaacaag 1860 ccgtactttt tataaatacc tctatgaata actgattaat ttagctttac gatttatttg 1920 gcagttacta ggtagtagct ggttatgtat attgcaagat tccctaagta cgaatctcga 1980 gaaacgcaag ccatagtaat ttatataatt cactgtggat tcgaagcgag aacagtttgt 2040 ttgttcaaat ttccaattga accaacgttt tatctattgc gacttcttat taagcagtac 2100 aatattagag acttaactac aatgatagct atatcattct acaaagtgtc actgtcacac 2160 ctgtattatt ccttaaaagg tacaacatca agtataatgt aaataacaca gcaataacaa 2220 aatagaagtt gctttgtagc agatggaacg ctaaatattt catttttgtt gctaaagcag 2280 ttttaataga atgaactatt gtgctactat agtgcatccc agtgcataaa cgtttaagtg 2340 ctagataaca taccgaagtg cagcaaattg aatttctagt aacacgctaa ttagtttttc 2400 attcctttag tgtagaatga aggaaaacac cggagctcca gaaagactac gatacgacaa 2460 cgtacttctc ttcaaatttt ccatatatta gaagctgcta aggctgaaac ggaaatcaaa 2520 ttttgcatta agcaattagg atttttggtg cagcttttca acgtaaatcg gaacgaagca 2580 tgcagctata ctcttcggaa aggtttgtgc aaattagggt taaataaaaa aatattttac 2640 attttggcca tattggaccg tttattaatc attaattgca gtgaatccga tgattctcaa 2700 tattgggttg attggttttt aggactaaaa ggaaacgaat ttttttgcga agtcgacgaa 2760 gacttcatcc aggatcgctt taatttgact ggattgagtc atgaagttcc acactactcc 2820 cagtctttag acctcatttt agatgtattg gatcctgatt taccagagga agtacaagat 2880 gaggtagaag ctagtgctcg tcatttgtac ggccttattc acgcccgtta tatcttaacg 2940 gctcagggtt tatataaaat ggtaagcaat tatgtttgta aacttctagt ttcctgtttt 3000 taagtttgga tacaatcttc gtgaacaaaa caggcattag acaagtataa tggacactaa 3060 gtgtgtaact tgacttatct ttcaattctt caattttatt acatttatgt cataccgtac 3120 tatttcgttt taattagctt tggattttga ctaacatcct ttctagcttg agaaatacaa 3180 aaaatgtgac tttgggcatt gccctagagt tttatgcaac ggacagccaa tgttgcccgt 3240 cggcttgtcg gatattgccc atacaaaatc cgttaaatta tactgtcctc ggtgtgagga 3300 tgtgtacaca ccaaaatcac aaagacatgc atccattgat ggtgcatatt ttggaacctc 3360 attccctcac atgctttttc aggtataccc tgaacttgcg gtccctaaat ctcaagaacg 3420 ctacattcca cgtatttttg gtttcaaagt acatagctat tctgctacct ttaaaaaaca 3480 agatgtatac aaagaaaagc aaaaaaagcg tttacaggga gccgaagctg aatcaaaaaa 3540 taaactggca ataacttaaa ggcattttgc ttaatcttca ttaacaatag aagttcaccc 3600 gattcttcga agtcccataa tcaccaaggc aaaaaatttt atttacatta tagctatgac 3660 agtttttact agaatgaact tttacgtttt tagatttttc ccgaagcatc agctcctttt 3720 atctaagagc gtaattttca aacttctaat catcattgaa gctaaaacct tttatgctta 3780 ttcagctttg aacctctttt tttactttga taattgcttt aataatttag ttttgatcat 3840 gacatatact gtggttaata tttatccact tctttcatca aattgttaaa ttgatcgtaa 3900 agacgaatgt ccttggaatc aggcccttca tcgagaagtt ccagcgctgt cttcagtaaa 3960 cgtttttcgg gatccagact caccaggtag ctagataact ccgtacaaac gtcccagttt 4020 tttattttta aagccatttc gtatatactt agaaaggtag aagcacaact ggctgaaccc 4080 tgatggatct gccatattat caaacactct gcggcacttt taacatcttc tgtttctaaa 4140 cactttttca ttaagtcttt tggttctccg aaatacttaa atatcatcgg ccagtattca 4200 gcttcagttt ttcgcaggca acccagaacg attttaaaag tggtaaactc ttgcaagtct 4260 ttaaataata aggatacttc ataaagcaat ttatctcgct gtgatgagtt gtaggtcaat 4320 aatgatgaag aaagtaattt ttctaacaca aaattgatat aatgcaagtg cccatatgtc 4380 ttgactaagt caatagcttg ctcacgttta tctaacagta atagtgctcg aacaatttcc 4440 ggaaggaatg gataaacacc tatttggata tgagcgtatt ccatcttatt aactaagttt 4500 ttatgaccct gggtataagc agttaataga agtccataat tgcttaatac agtcagcaaa 4560 catccctgag attccagatc tatacattcc ttttgaaact tttcagaaat atttacattt 4620 ttaatcaatt tagcaagatg tgtgttatca aagttacaat caaccccatt tttgtagaca 4680 aagctgggcg atctgaggtc ttcaacagat aaacgatgaa aattttctag agatactctt 4740 tttgatgtat tgagaaactt tggaaggcca tctataaggt ttttagtatt tatcaaaaca 4800 gaggaaagga tctcgctaaa agataaccat agcttaagtc cctttcccgt catgatccat 4860 atagaatgaa ataatgaagg gatttcttga cttccattga taaagtaaaa ctcaacgtct 4920 cccgctagca tgctgcactg atataaaagc tctttagaat gttgtttttt tagacttaac 4980 aaaacaagtt ttccattaat gagaaccaga aggactgcat aaaaaagtaa atcagaaggt 5040 tgaatatttg caaggtcttt tggtagtaat aacgttatgc ttcgaactct ggatggagta 5100 gtgaatattg gagcaaaatt tacggatgtt aaatagtcga gttctaatct tcctaattcg 5160 tttatatcga atctgatgtg atggaggaaa ttatcagctg tatagacaac caaagaatat 5220 tcgtcgcaga ctgacattgt cacaattgtt gaagtaaacg ataatttagc caagttttct 5280 ctatcgtcta aaggcccttt tgctttgtat aggtgtagct caaagttgga ctcgcaaaca 5340 acaccggcta gcaaaaattg tgagcaccaa atcattggac aagtaacact gattaattgt 5400 tccatattgg catctttata taagaaccag gtcttttttg agcaaacata tatagctaat 5460 ccatgaagcc cagcgattgc tatcaaactt ccatcatctt tgatagaaac atatctaata 5520 ggccattcag aagcaacata tggttgagga tactcaactt gatcccaaaa gtcaatgcca 5580 aaattgtttt taacgctaga acaaaattca tatgacttcg aaatatataa ccgatcgcta 5640 gtttgagctc cacaaacttg caaggtgctt 5670 //