ID SPAC16A10 standard; DNA; FUN; 16963 BP. XX AC Z97185; XX SV Z97185.1 XX DT 25-JUN-1997 (Rel. 52, Created) DT 01-APR-2003 (Rel. 75, Last updated, Version 5) XX DE S.pombe chromosome I cosmid c16A10. XX KW myb1; rho-like gtp binding protein; taz1; telomere length regulator; KW transcriptional activator; tRNA-Lys. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-16963 RA Murphy L., Harris D., Wood V., Barrell B.G., Rajandream M.A.; RT ; RL Submitted (24-JUN-1997) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Hinxton Hall, Hinxton, Cambridge CB10 1RQ E-mail: barrell@sanger.ac.uk XX DR GOA; P79005; P79005. DR GOA; P87293; P87293. DR GOA; P87294; P87294. DR SPTREMBL; P87294; P87294. DR SPTREMBL; P87295; P87295. DR SPTREMBL; P87297; P87297. DR SPTREMBL; P87298; P87298. DR SWISS-PROT; P79005; TAZ1_SCHPO. DR SWISS-PROT; P87293; YDN1_SCHPO. DR SWISS-PROT; P87299; YDN8_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S.pombe) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c16A10 is overlapped at the 5' end by cosmid c24C9. XX FH Key Location/Qualifiers FH FT source 1..16963 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c16A10" FT /map="IL" FT misc_feature 1..82 FT /note="nominal overlap with cosmid SPAC24C9, EM:Z98601 FT S.pombe chromosome 1" FT CDS 442..2934 FT /db_xref="GOA:P87293" FT /db_xref="SWISS-PROT:P87293" FT /gene="SPAC16A10.01" FT /product="hypothetical protein; similar to S. cerevisiae FT YJL108C; S. cerevisiae YJL108C is regulated by pheromone; 9 FT predicted transmembrane helices" FT /protein_id="CAB10002.1" FT /translation="MGVQLKLDPNSKNWLRQPDQQPIQDSICTLPGPGSLLKKKIRKTE FT FRIDNGLLGNTSPIINVESKIPFHRRANSTPEESRKRVSFSLTSNDGAKSDRSSQKSSN FT SRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEEDGNFASIPELHEDNEYHSNLA FT TSSNPFSDGSSSKSASLTASSAAASPTVSFSPASTSENLTPTSSKSLASNTSLVQSFNS FT ASRSSSISGNQYTYNLLGKSTDESPKITISAGTSMSHFPSASSKLIQMRRHAHMNSEKC FT DPELLSHNEYFPMMKRGFISRFLCLFVSRDANVYIGPLESSHAKPYRPFGSFVSRFTNR FT VASDLESQIPDALEAVLTQNNDYDFNSFLHNMGYSRCYVDENYHITIHVSSYERRVEFM FT LAVCEAMMLYGSPSHKIQQSLRIASRILQLPATFLYLPDCMFVYFKKLEQYSPDVFVVR FT VTSQTDLNRMVLVNEIFRRVMRDKLSAEDGTEVLRNITSFRPLYRDWLVAFMHGVASAS FT ILPVVYGGGWRDMLIGFVLGLLLGIFRVYINPRFFLFDSLFEVIISIILSFLGRAFGSI FT SRYDKPVFCFAALVEGAITLILPGYVVFCGVLELQSKNIVAGGVRMLYAVIFSLFLSFG FT ITIGSALYGWMDKDATDADTCMSIIAVSPYWYILLIPIFTLSLLIVTQSHPRQWPIQMF FT VACCGYVVYYFSSLHFGASQISSAIGSFAVGCLGNMYSHFIKSSSFAVVLPAIFVLVPS FT GFAAQGGVSAGLDTASQITSKNTTTNTTTVTTENSQNSSLEFGFTMVEIAIGIAIGFLA FT SSLTVYPFFGYRRKNMML" FT CDS join(3778..3870,3921..4238) FT /db_xref="GOA:P87294" FT /db_xref="SPTREMBL:P87294" FT /gene="SPAC16A10.02" FT /product="putative transcriptional cactivator p15(PC4); has FT a bipartite structure composed of an amino-terminal FT regulatory domain and a carboxyl terminal cryptic FT DNA-binding domain (Pfam); regulated by protein kinases FT (Pfam); involved in the release of TFIIB from the FT transcription complex during transcription initiation FT (SWISSPROT); binds to TFIIB (SWISSPROT); similar to S. FT cerevisiae SUB1" FT /protein_id="CAB10003.1" FT /translation="MAGKRVIASGDKASSKKPKTEKQSDHELHWALNETEKKRITLSEF FT RGTRYVHIREYYEKDGDMLPGKKGIALNINEWKKLKQLIHEVDDSLGLVDSGSDSEDEI FT KEKAENNASLKNDEDDLNTKTENKTDDSSVEN" FT misc_feature 3871..3876 FT /note="gtaagt, splice donor sequence" FT misc_feature 3899..3920 FT /note="ctaataaattgttttggattag, splice branch and acceptor s FT equence" FT CDS join(complement(6891..7010),complement(6668..6847), FT complement(4334..6616)) FT /db_xref="SPTREMBL:P87295" FT /gene="SPAC16A10.03c" FT /gene="pep5" FT /product="putative vacuolar sorting protein; peripheral FT vaculor membrane protein; similar to S. pombe SPAC823.12 FT (paralog); possibly involved in vacuolar biogenesis; FT similar to S. cerevisiae PEP5; zinc finger protein; FT zf-C3HC4 type (RING finger)" FT /protein_id="CAB09996.3" FT /translation="MNINEVDQTYRSLLTLKQWEKFSLFQWQECPSIKILHDSVGNKIS FT CIGKSTKRIAIGTLDGRIVILNSRLQLIRDFYACEQGIVQQIYITADQSALCCVVLDKQ FT NFVYLQFWSLNPSKKTNSNSPLCLYEHRLYGIPNPPFPATSLYVSIDIKTVVCGFANGL FT VIRVEGDFVRDLGSRQDIILREKDSITNLILYSPKKLFVSTTTQVMVYKIKNNTKKVIS FT NHGIPLFCSIQYQGKYIMCAGGSFLSVYTTPDMQLQNTYCVDGTFELLFSSFGLVFVVY FT TRKNGENGLENNSSIREIKALDVEKRYVLYESLLEQSYDNIFFNSFDCIFFSSTKVPCQ FT LIRLPSDFVLCKMKGKKEHKDAFKIANYLGSPEDTIRECALAAAGECRQQLNFQDATYY FT YIEAIPFSDSAEIIKFYLEKKLIKELTSYLEALSAKGFAFSHEISTLIYLYIKLRKLDK FT LTEYVSGCPTEISLPILRKYKCLDQMELLGTIRKLPNVCMEVYQEKGDVEKAFNHLQVC FT NLPELLRTSNSFGIWLFNSDPMRFMKEAIRNIEILNSQGKDKELSNILKIVYLGIFSQN FT VQIQLIFLDELLKSKKSENVLKFIYTRKLYALMQKELQHSNPQNELDALQIIHDSQGLL FT DYESSILCLQAVSWKQVTDLLYSHLSLKEGQDDSLIQQIISDPETVKTLSETYSSEDAL FT HVLKFFVRERSITNKYEDILYKILEACFMQFRIPIQHVLNILVKDGTLNFCFLKPLLLK FT WMNDYETRIHQNDDEIQVIKNDIEKKRQLLGTIQDSEKVCDNCEGLLDVPFVSYSCLHL FT VHRDCATETVCPKCKAGYLDKKNSHDQKKTSTFSELFHDFESIDSVML" FT misc_feature complement(6617..6642) FT /note="ctaatataatttatgttggttgtag, splice branch and accepto FT r sequence" FT misc_feature complement(6662..6667) FT /note="gtaagt, splice donor sequence" FT misc_feature complement(6848..6862) FT /note="ctaacatgaaattag, splice branch and acceptor FT sequence" FT misc_feature complement(6885..6890) FT /note="gtacgt, splice donor sequence" FT 5'UTR complement(7011..7433) FT /note="mRNA from AU007677" FT tRNA 9076..9158 FT /evidence=NOT_EXPERIMENTAL FT /note="tRNA Lys anticodon CTT, Cove score 74.69" FT /gene="SPATRNALYS.05" FT /product="tRNA Lysine" FT CDS join(9804..9822,9893..9934,9996..10042,10150..10353, FT 10470..10604,10661..10810) FT /note="coordinate change 31.034.2003 N-terminal exon FT replaced" FT /gene="rho4" FT /gene="SPAC16A10.04" FT /product="Rho family; RAS small GTPase; similar to S. pombe FT SPAC20H4.11c (paralog); similar to S. cerevisiae RHO1; FT guanine nucleotide exchange factor" FT /protein_id="CAD86931.1" FT /translation="MSAFKKKTSKKLVVVGDGGCGKTCLLIVFSSGTFPERYVPTVFEN FT YITDITYGPNSKVIELALWDTAGQEEYDRLRPLSYPNSNVILLCFSIDCPASLNNVTEK FT WYPEVQHFCPRTPIVLVGLKADLRKDRNATEVLRTQGLTPVTYQQAQSVALSMNAPYVE FT CSAKENTGVNEVFQLAVGLTIKKSFSFSKKSCVIL" FT intron 9823..9892 FT /note="confirmed, PMID:12653963" FT misc_feature 9881..9892 FT /note="ctaatttttcag, splice branch and acceptor" FT misc_feature join(9904..9934,9996..10042,10150..10353,10470..10604) FT /note="Match to PF00071 ras, Ras family Score 167.63" FT misc_feature 9935..9940 FT /note="gtatgc, splice donor sequence" FT intron 9935..9995 FT /note="confirmed, PMID:12653963" FT misc_feature 9978..9995 FT /note="ctaatgcttgatgtatag, splice branch and acceptor seque FT nce" FT misc_feature 10043..10048 FT /note="gtatgc, splice donor sequence" FT intron 10043..10149 FT /note="confirmed, PMID:12653963" FT misc_feature 10135..10149 FT /note="ctaacatcatgctag, splice branch and acceptor FT sequence" FT misc_feature 10354..10359 FT /note="gtatgt, splice donor sequence" FT intron 10354..10469 FT /note="confirmed, PMID:12653963" FT misc_feature 10452..10469 FT /note="ctaacatttttctttcag, splice branch and acceptor seque FT nce" FT misc_feature 10605..10610 FT /note="gtatgt, splice donor sequence" FT intron 10605..10660 FT /note="confirmed, PMID:12653963" FT misc_feature 10648..10660 FT /note="ttaacatatttag, splice branch and acceptor sequence" FT CDS complement(11819..12076) FT /db_xref="SPTREMBL:P87297" FT /gene="SPAC16A10.05c" FT /product="putative spindle component; putative spindle pole FT body component; similar to S. cerevisiae DAD1" FT /protein_id="CAB09998.1" FT /translation="MDITENIQNEQNKDFDDIDFERRRRLLTLQISKSMNEVVNLMSAL FT NKNLESINGVGKEFENVASLWKEFQNSVLQKKDREMLDAP" FT CDS join(complement(13299..13463),complement(12878..13051), FT complement(12349..12813)) FT /db_xref="SPTREMBL:P87298" FT /gene="SPAC16A10.06c" FT /gene="reserved_name_pli2" FT /product="zinc finger protein; zf-MIZ; possibly involved in FT DNA repair; similar to S. cerevisiae MMS21" FT /protein_id="CAB09999.1" FT /translation="MSEAQLKTSLEALSQNLLPGNQNHCSFDFQLKEIDDSIKQVIKCA FT LVAAEIKNNECLDMLDSGIRELLDAKQRLLLMQQSVDTLANKTSENISDFEVRTTEMTF FT LLFLLYVDENKSLLDIYTQIFKELIQEYEEKSDYGKYGTQGEYIEFKKTIWHEQNTDGS FT DFPSMKTFFNVMNTEEQEADEVMVYSATFDNRCPLTLQPIVHPILSTACNHFYEKDAIL FT SLLNPTCVCPVVGCEARLQRSLLKEDEILERRLRRAQEISNLKEA" FT misc_feature complement(12814..12826) FT /note="ctaacaaaattag, splice branch and acceptor sequence" FT misc_feature complement(12872..12877) FT /note="gtagat, splice donor sequence" FT misc_feature complement(13052..13069) FT /note="ctaacacttgccttttag, splice branch and acceptor seque FT nce" FT misc_feature complement(13293..13298) FT /note="gtatgt, splice donor sequence" FT 3'UTR complement(13787..14002) FT /note="mRNA from SPMYB" FT CDS complement(14003..15994) FT /db_xref="GOA:P79005" FT /db_xref="SWISS-PROT:P79005" FT /gene="myb1" FT /gene="taz1" FT /gene="myb" FT /gene="SPAC16A10.07c" FT /product="involved in telomere length control (PMID FT 9034194); required for telomere clustering during meiotic FT prophase (PMID 9572143); involved in recombination FT (required) (PMID 9572143); TRF family protein; involved in FT meiosis (PMID 9572142); involved in sporulation; Myb family FT DNA-binding protein; binds telomeric DNA as a dimer (PMID FT 10606652); involved in telomeric silencing; protects FT chromosomes from Ku-dependent end-to-end fusions (PMID FT 11172711); interacts physically with Rap1p (PMID 12018857); FT similar to S. cerevisiae TBF1; similar to human TRF2" FT /protein_id="CAB10000.1" FT /translation="MISVQSTETIQKVLENEGDQQFDKEVVQNSDSNIETGQISDSLTK FT AVEERAETESSSNLSNFTTSESESSKPAYCFNSHSQNMAEGSISIPVISHSMNVENEVS FT TAEGQDSRTGESENDQNAMIVRSIWDIEKASLLVNDCQNIANMAEQKVMMVSAIFSESS FT KDIVNPESFSERLGKETVKDLYEFNEQLTTKYGLEFRTIFFSYIRKYDAYWCLFEDLEK FT PLKSIQFFTGLIDLLDNTNKHLTLRSIVLDALLSADEEDSFYGDALVLFEELVIRYFGT FT DSNPSIDASEFILSCLPYTSLDALNVVCGQVWKSQKICDFLKSTIGNTSNSPLQLRASF FT PAFVNAVIHFLLEFKNVRRLERKDLSVKGMLYDSDSQQILNRLRERVSGSTAQSADEAS FT GHESDASEDTFSERTLGLNSIDNTEISEVVSLGLVSSALDKITGLLSADNLSETVSQAR FT DFSHTLSKSLKSRAKSLSQKEAANRSKLIAKRGDNLRREASLSSEQDDLSEDFPPVRES FT DEQESRSGGRSSAMRVSIERSAARSGTRRSQGNPYEGYRTRRKWTDEEENELYEMISQH FT GCCWSKIIHIQKLENGPLKTFGPTQIKDKARLIKARFMKQNRLQELYSKSLNWKNVTVG FT QAYCELHKIPYIEATPPLLREELVNYQS" FT misc_feature complement(14288..14314) FT /note="PS00037 Myb DNA-binding domain repeat signature 1" FT CDS complement(16739..>16939) FT /db_xref="SWISS-PROT:P87299" FT /gene="SPAC16A10.08c" FT /gene="SPAC589.01c" FT /product="hypothetical protein; sequence orphan" FT /protein_id="CAB10001.1" FT /translation="DSNQIQELENLLRKEKQRNTEHEKIIRRMKKELKELHSQFNFAKK FT LFISALSANQDILDKMNDEFN" XX SQ Sequence 16963 BP; 5428 A; 3042 C; 2758 G; 5735 T; 0 other; gtgttgtttt aggccatata ttttcctttc gaaaattctt caataggatt aggattgttt 60 ggcatcaatt ttgtcattga tcagatatct tcattatctt ttcattttca ttaaagcctc 120 cataattaga tactaataag aaaggtgaaa ttttcttccc gcttctccca aaattgttcc 180 tctttcaaat taaaagaaaa ttcaaaagtt tctgttaact cgtgcactct ttgttttcct 240 ctctctttgt gtataatctg tcgaagtttc ctattacatt ttatttattc actatttatt 300 tcctacagtc gtttctttac tacagtatta aaccctttat ttattatttc ttaacccccc 360 aaaaaataac acaaaaacga aaacgattga cactttttta ccagccgctt tctaaccttt 420 aaatttcttc tactattttt aatgggagtc cagcttaagt tagaccccaa tagtaagaat 480 tggctccgcc aacccgatca gcaaccaatt caagacagta tctgtacact tcctggacct 540 gggtcccttt taaagaaaaa aattcgtaaa accgaatttc gtatcgataa tggattgttg 600 ggaaatacta gtcccattat aaacgtggaa tcaaaaatac cgtttcatcg tcgagctaac 660 tctacgccgg aagaatctcg aaaacgcgtt agtttctcgc ttacttctaa cgatggagca 720 aagagtgacc gttctagtca gaaaagcagt aattccagaa aatcgattag atcacagtct 780 cgatcccgta gctcctctgt tggtagtgat tctcaagctt ctattcaatc cggtacaaac 840 agcgaagttg tcagtgatga tgaggaggac ggtaatttcg cttccatacc agaacttcat 900 gaggataatg aatatcattc aaatttggcc acatcatcca atccattttc agatggcagt 960 agttctaaat ctgcctcatt aaccgcctca tcagctgcag cttctccaac cgtatctttt 1020 tctcccgcct ctacgtctga aaatttaact cccactagca gcaaaagctt agcttcaaac 1080 acctcacttg ttcaatcctt caactctgca tcccggagct catctatttc tggaaatcaa 1140 tatacctata atttgctagg caaatcgact gatgagtctc caaaaattac tatctcggcg 1200 ggaacatcaa tgtcacattt cccctcggct tcatcaaagt tgatccagat gcggcgtcac 1260 gcccatatga acagtgaaaa atgtgatcct gaattgttgt cccataacga atattttcct 1320 atgatgaagc gtggtttcat tagtagattt ctctgcttgt ttgttagtcg tgatgctaat 1380 gtttatattg gccctttaga atcatcacat gcaaaacctt acagaccttt tggcagcttc 1440 gtctcgcggt tcaccaaccg agtagcctct gatttggaaa gccaaatacc tgatgcgcta 1500 gaagccgtat tgacccagaa caacgattac gattttaata gttttttgca taacatggga 1560 tactcacgat gctatgttga cgaaaattat cacatcacca ttcatgtttc ctcttatgaa 1620 agacgagtcg aatttatgtt ggcagtgtgt gaagctatga tgctttacgg ctccccaagt 1680 cacaaaattc aacaaagttt gcgcattgct tctcgcattt tacagcttcc tgctactttc 1740 ttgtaccttc cagattgtat gtttgtttat tttaaaaagc ttgagcagta tagtcctgat 1800 gtttttgtcg ttcgcgttac atctcaaacc gatttaaata ggatggtttt agtaaacgaa 1860 atttttcgaa gggttatgag ggataagttg agtgcggaag atggtacaga agttttgcga 1920 aacatcacat cttttcgccc tttgtacaga gattggcttg ttgccttcat gcatggagtt 1980 gcgagcgctt cgattctacc agttgtatat ggaggtggat ggagagatat gttgataggc 2040 ttcgtgctcg gtctattgct tggtatattt cgtgtttaca ttaatcctcg atttttctta 2100 tttgacagtc ttttcgaagt tattatttca attattctat catttctggg cagagcattc 2160 ggaagtatca gcagatacga caaacctgta ttttgttttg ctgctctcgt tgaaggagcc 2220 attactttaa ttcttccagg ttacgtagta ttttgtggcg ttttggagct tcaatcaaaa 2280 aacattgtgg caggaggtgt tcgaatgtta tacgccgtca ttttttcatt atttttaagt 2340 tttgggatta ccattggatc tgctctgtat ggttggatgg acaaagatgc taccgatgct 2400 gatacatgta tgagtataat tgcagtcagt ccatactggt acattctttt aatcccaata 2460 tttacgttga gtctattaat cgtgacccag tctcatccta gacaatggcc tattcaaatg 2520 tttgtggcat gctgtggata tgtggtgtat tacttttctt ccttacattt tggtgctagt 2580 cagatatcaa gtgctatagg ttcatttgca gtcggatgct taggaaatat gtactcacat 2640 ttcattaaat cttcttcttt tgccgtggta cttccagcaa tctttgttct agtaccttca 2700 gggtttgcag ctcaaggagg tgtcagtgct ggacttgata cagctagcca aattaccagc 2760 aaaaatacaa ctacaaatac tactactgtt acaacagaaa attcccagaa cagcagtctt 2820 gaatttggtt ttacaatggt tgaaattgcc attggaattg caattggatt tcttgccagt 2880 tctcttactg tgtatccgtt ctttggttat cgacgaaaaa acatgatgct ttaaataagt 2940 atttccaatt cgcgtgttgt tgaaacttag ttctcaacta cacgttaatt ttcaaaaaaa 3000 aacatgttgc ttgcctgttt gttttaggta ttgctacaat tctgtgtttt aatttatatt 3060 gtatcaagac gatagatatt cttctcactc cgttatctac ctgacattgt atatttcacc 3120 gcgtccttta tatgttccag ttcttttttt tttcaatcta actattcatt taactcatta 3180 cttttggaat gtctattgct aaagtaaatg ctgtattata ttattactac tgttaatatt 3240 ttaataattt aattcaggcg ttgtgaaaac ttttgatgcc tgtcccatta taagtaaatt 3300 ttgttattat tagatataac tgaaaaactt aatatatgag atctaatcgt attccattac 3360 ttttgcatct ctaaatgtat actatttgtg gggatacgct tgcctttgcc tatgtaaatt 3420 aaattgcaaa cggaatgtaa ttttcaataa atcattcaca agcaaattca aggatggcga 3480 ttaaatatgg gttgcagtaa taacaggcaa aggggttttt tcatattaat cataagtgtt 3540 ctataaatgg atttaggtga aaaaagtccc cacgtgagta tcctaaaatt catacccagc 3600 cgaaaagcac ttatagtaag tacgctaaac ttcgcatact ttaaatactc ataaattatg 3660 acaagtttat actcacatcg gtaagctgat aacaccgcta cacatagcaa ctccttatgt 3720 ggtgaatgtg ttaccatcct tggagtcaaa atgtgatttt accaatacga tttaaatatg 3780 gctggaaaac gagtgatagc ttcgggagac aaagcatcca gcaaaaagcc taaaacagag 3840 aagcaatccg atcatgaatt gcactgggca gtaagttctt aaaagaaatt ctgcgaaact 3900 aataaattgt tttggattag ttgaatgaaa cggaaaagaa aagaataact ttgtcagaat 3960 tccgtggtac tagatatgtt catattcggg aatattatga aaaagatggt gacatgctac 4020 caggaaaaaa ggggattgct ctcaacataa atgaatggaa aaaattgaaa caacttattc 4080 acgaggtaga tgattcttta ggattagtcg atagcggctc ggattcggaa gatgagatta 4140 aagaaaaagc tgaaaacaac gcatccttaa aaaatgatga agacgactta aataccaaaa 4200 cagagaacaa aactgacgac tcttcggttg aaaattaaac agtgttgtga ttcaactgat 4260 gagtaacaaa agaaaatctt ctcgtttaat cagattagat tcaagcaatg gcaaacgttt 4320 catctattaa acattacaac ataactgaat caatagattc aaaatcgtga aataactcag 4380 aaaacgtaga cgtttttttc tgatcatgtg aattcttttt atccaaataa cccgccttgc 4440 attttggaca tacagtttca gtagcacaat cacggtgtac taaatgtaga caactgtaac 4500 taacaaaagg aacatctaag agaccttcac agttgtcgca aactttttct gaatcttgta 4560 tagttcccaa aagctgcctt tttttttcta tgtcattttt aattacctgg atttcatcat 4620 cattttgatg gatgcgagtt tcataatcat tcatccattt aagaagtaaa ggcttaagaa 4680 agcaaaagtt caacgtgccg tctttcacca gaatattcaa aacatgttga atggggatac 4740 gaaattgcat aaaacaagcc tccaaaatct tatataaaat atcttcgtac ttgttagtta 4800 tagatcgttc acgaacaaag aatttgagaa catgaagggc gtcttcgctg ctatacgttt 4860 cactaagggt ttttacggtt tcaggatcac tgattatttg ctgaattaag gaatcatctt 4920 gaccttcctt aagagataaa tgagaataca gtaagtctgt cacttgtttc caagagaccg 4980 cctgcaaaca aagaatggac gattcgtagt ctaaaaggcc ttgactatca tgaataattt 5040 gaagtgcatc taattcattt tgggggttag agtgttgtag ctctttttgc attaatgcat 5100 atagtttccg tgtgtaaatg aattttagta cattttctga tttcttgctt ttcaaaagtt 5160 catcaagaaa tattaattgt atctgtacgt tttgactgaa aattcctaaa tacacgattt 5220 taagaatatt tgacaactcc ttatctttgc cttgggaatt tagaatttcg atatttctta 5280 tcgcttcttt cataaacctc atcggatccg aattaaataa ccaaatacca aatgagttag 5340 atgttcgcaa caattccggt aaattacaaa cttgaaggtg attaaacgcc ttttcaacat 5400 cacctttctc ttgataaact tccatgcata catttgggag ttttcgaata gttcctagca 5460 gctccatttg atctaagcac ttgtatttcc ttaaaattgg caaagaaatt tcagtagggc 5520 aaccgcttac atattcggta agcttgtcca attttctaag cttgatatac aagtatatta 5580 acgttgatat ttcatgcgaa aacgcgaacc ccttagcaga aagtgcttcc aaataagaag 5640 tcaattcttt aattaatttt ttttccaagt aaaatttaat tatctctgct gaatcagaaa 5700 acggtatagc ttcaatataa taatacgtag cgtcttgaaa gtttaattgc tggcgacatt 5760 cgccagcagc tgcaagagca cattcgcgaa ttgtatcttc aggagaaccc aggtaattag 5820 caattttaaa tgcatcctta tgctcttttt ttcccttcat tttgcataaa acaaagtcag 5880 atggaagacg tattaattga caaggaactt tagtcgaaga aaaaaatata caatcaaaag 5940 aattgaaaaa aatattatca tagctctgtt caagtaaact ttcatataga acatagcgtt 6000 tctctacgtc caatgctttg atttcgcgta tagatgaatt gttttccagt ccgttttcac 6060 cattttttct agtgtatacc acaaaaacaa gcccaaaact agaaaatagg agttcaaatg 6120 taccatcgac acagtatgtg ttttgtaact gcatatcagg agtggtataa acagaaagaa 6180 aggagccacc agcgcacatg atatactttc cttgatactg aattgaacaa aataaaggta 6240 taccatgatt ggaaatgacc tttttggtgt tattttttat cttataaacc ataacctgag 6300 tcgtggtaga aacaaatagt tttttaggtg aatataagat aagatttgtt atagaatctt 6360 tttctcttag tataatatct tgtcttgatc ccaaatctct tacaaaatca ccttcaacgc 6420 gtataaccaa tccatttgca aatccacaaa ccacggtttt aatatcaatc gaaacataca 6480 gtgaagttgc aggaaaaggt ggattcggaa ttccatatag tcgatgctcg taaagacata 6540 aaggtgagtt tgagtttgtt tttttagatg gattcaagga ccaaaattgt aaataaacaa 6600 agttttgctt atcaagctac aaccaacata aattatgatt agcatttaaa catatcatgt 6660 gacttacaac aacacagcat aaagccgatt gatctgcagt tatgtagatt tgttggacaa 6720 tgccttgttc gcaagcgtaa aaatcacgaa tgagttgcaa acgagagttc aaaataacga 6780 ttcgtccatc caacgtgcca attgcaattc tctttgtact ttttccaatg caactgattt 6840 tatttcccta atttcatgtt agtgtacact tcctatactt ttaaacgtac cactgaatca 6900 tgcagtattt tgatacttgg acattcttgc cattggaaaa gtgaaaactt ctcccactgt 6960 ttcaatgtta gtaaggatct atatgtttga tctacctcgt ttatattcat aaaactatca 7020 agtttttgat caaaattatt tacgctgtag gtgaaatccg aggatttctt taaaccacgt 7080 tccagttttt taaaagcttt aagaggaata ataaatgttt aggtgcagta ctactgtact 7140 acataaaata acgagtatta ctgaggtaac acttactgtt ctatacagtt attactataa 7200 tacttagtac aacagataca attaagaata acttgtaatg gtattcacat tactaataaa 7260 cttctaaatt catctgtttg ggtattcatt acatattcat caactaagca agtcttacat 7320 gcgtccaaat ttgtttaaga caagaaaata taccatctat gcttaataaa caaacttcat 7380 ctcttctagt acgcaaataa atacattaaa tttattttac attataataa gattcatcac 7440 tttctcaaaa ttttgaaacc ggaaacggaa ttcagcatgt tttaataaaa agatgagcaa 7500 ccgcgttctt actaaatacg caattctgat aatttgggaa ttgaatagtt agtaatttta 7560 aatttaatca ttaaaacata ccaaaaaatg ggcccgttcc ttttaacatt ttgctcagac 7620 caagtgaaaa aaacctaaaa cgcgctcaag aggaacaacg gacgagctac actcaaaata 7680 ttaatgaatg ggtagtcaac tattttacta gcgaacaagc aaaagctttg atccatggat 7740 ctcacgtaat gtaaaatagt atcttaccct aagcattatt tttttgagaa acaggacgtt 7800 cattattgtt tgccgcaaag aatccaagtt caaggaaatt gaatacagac cagagttgct 7860 attcgtttga atgtacattg tgaccacaag tcggtttttg tttccttcca aatagtacct 7920 tcaacacgaa gttgtacatt tagcacggac aaagaatcga ccaaaaaacc aaaattaaat 7980 gtagaaggaa aaccttcaag tcattttttt tataatagct cttttaagtt ccatactttt 8040 tatctccgta acaaatgcca aaagaagcta gtatagacga gagcattcag ccaacaaatt 8100 tgcacaagca aaaattgtta ccaatacaat ttaatatttg tatggggaat tgaaactatc 8160 tataaaactt tcttcattgc ttgattttta gtaacgttca gtaattcaaa cgagactctt 8220 ctaccaaaaa ttcgttgtga tctgacaagc acttaaatga tggatacaga ttttatacaa 8280 atgaaaggta ggattaaact gtaaatgaat tttaaaggca gaagactcac gtacactgca 8340 cccagcgatt caattgatgt ccaggcctct tttttcctac tgtatacaga agtatgaagt 8400 ctgcagccat aataaaagcg gtaggaatga tctatgtttt ccgcaacgcg tcaacgattc 8460 catgaagagc ctctcttatg aatattccta atgatcaaaa ctctaaaaaa agctaaaaag 8520 aataatttcg caatgctgaa aagttgaaga gtacggttac acgaagcagt acaggaattc 8580 gaaatccatc gaaaacaact atccagacaa actgaaaagc taaacagact actcgttctt 8640 tctagtagac agtatacagg ttttggaata gcaaactata cttaaggcct atgaatcatt 8700 tccttcaaac tttcaaatca atcaccaagc cctcaagaac cgtttgatta attttccttg 8760 tcaaccttct aagcatgggc gttgcgttga gtataaacaa aacagcaata aatccgagta 8820 tacatgaatt gtttccttct tcaaacaaaa cgctgttacc tttgcgcgta tgatgacttc 8880 ccatacttac aaaaattttc aaaacctaac agtaatctat aaattttaag ggctaacatt 8940 acattcaaaa gttattagaa tgttgctaat aaatagccag aacattattt ttattattca 9000 ttctcatttc ttacttattt aagtgaatta aagaaaatgc gtaaattaga tttaatccgt 9060 taaagtacaa gcaagtcccg agtggctcaa tcggtttaga gcgtctgact cttatgatgg 9120 tcatcagaag gttgcgagtt cgagtctcgc cttgggagtt aattttttgg ttatggtact 9180 tcaaggccaa gtagaataat caattaatta ttatgtattt ttacttcaat aatttagttc 9240 ccaaaacccg ttactttatt aattagtata agctgatgaa ttcacttatt gagtcgccaa 9300 cttaacgatg aggtgttaga ctagcctgcc atatatactt ttggcggtaa atatttcaat 9360 ttcgttaggt atttttatct aactaatgta cacgtttcta gcaacctgaa atgtaggctt 9420 gtagaactag ttgaaaacag aacgtttgga ttatatgaat gataatgaat acaccgagca 9480 tcttttatta ggcagactca acaattgaca actttgcaat gtccattgaa tcctaaaaat 9540 aaaaatagac aggtatatat acctataatt tttttggtgg ctaacaacct aactttttca 9600 attaaaacaa ttaaccggca tagtccttcg taaattccat gcacacttga taaatttaaa 9660 tatgtttcat cgcggatatt ccgtttactg ttttatatat ttatttacct attgtttacg 9720 tgctgcgttg tcatggcaat gtttagcttg gaaataccag caatctcagc tggtaagcaa 9780 acattaacaa caacaaaggc aatatgtctg ctttcaaaaa gagtggatcc aagtcaggta 9840 attcaattgt tcctttttct caaatatctt aaagtttttg ctaatttttc agagacttca 9900 aagaaacttg tggtcgtagg agatggcggt tgtggtatgc ttgataaata taggtgaatg 9960 acagctggtc taatgtacta atgcttgatg tataggaaag acatgtcttt taattgtatt 10020 ctcaagcgga acttttcccg aggtatgcca atagatcgaa caataactgt ttaagtgcag 10080 tgttcatatg attcatgtac tcagttcact tgaattatct aagtatactg atttctaaca 10140 tcatgctaga gatatgtgcc tacagtgttt gaaaattata tcaccgatat aacatatgga 10200 ccgaacagca aagttattga acttgctttg tgggatactg ctggtcagga ggaatatgat 10260 cgtttacgtc ctctaagtta cccgaatagc aacgttattt tgttgtgttt ctcaattgat 10320 tgtccggctt ctctgaataa cgttacggag aaggtatgtg ctctataatt tatattgcta 10380 aacgtagctt tgggtttctt caatgtcatt tgcgtctagc cattagtatt tcatctttct 10440 catattattt tctaacattt ttctttcagt ggtatcctga agttcaacac ttttgcccta 10500 gaactcccat cgttctcgtc gggctaaaag cggatttacg caaagatagg aatgctactg 10560 aagtactaag aacccagggt ttaacaccag taacctacca acaagtatgt caatgataag 10620 ctaacttttt tctttcgttt atttttttta acatatttag gctcaatccg ttgctttatc 10680 aatgaacgct ccatatgtcg aatgctctgc caaagaaaac actggtgtaa atgaagtttt 10740 tcaacttgct gttggcctca ctataaaaaa atcattttcg ttttctaaaa agtcttgcgt 10800 gattttataa tatcttgatc atggccttta tttacttaat cttaccattt ttgatgtctt 10860 catttttgct tcgcctattt gtatagcatt tgatgagatt gttggaacat aagaagcaag 10920 aaaaacgctt tatttacgca ttctaacatt gctaattgca tataaaccaa tactccaaac 10980 gtttgctgca atagagacat tatgcttgta tgctttcttt taatctaagc atattgtgta 11040 gcagtttcct tttcagaatt ttgctcctat ctcaactttt tatcttttca ggtatttaac 11100 tagccaatat ggtttagatg gattatcgac tattggagct attttgtgat ttataatttg 11160 tttattaaga cgttatctgt ttgattctga atatatgtca gcaaaaatca attggtctta 11220 atgttttttt ttacttttac ttcaaatatg cctatatttg tccgaaactt tgaaaatcgg 11280 ttttttaacc ctgactggtt cagggtattc atgtcgtgaa taaaacgata gttatataga 11340 agatattgaa atgaatactt atgattcctt ttaccttttt attatgctaa actgcattaa 11400 acccctttta taatttaaat aataccatta aaatggtaat ttctaaaagg attcgtatag 11460 tagtaaagaa aaagtttact atgcaaatat taacatcaac agttgttctg cctctcagac 11520 gatgaacggc ttatttaaaa attggcagct tttagaagaa tataggaaga ttaaaaacta 11580 atatatatat acaaacacat tattaatgaa gtgacttaag gtttgtgtat tgtaatatgt 11640 aagttaattt aaaagaatcc tgacctttga ccaatttttg aggacgctat taaatttata 11700 gattcataaa aaacatataa taactaataa tttaatttaa tatgcaaatt ctttgaaaca 11760 aactgcgtta aggcatatgt ttagcggcaa tgaaaataca tttagaaaag aacgaccatc 11820 aaggtgcatc tagcatttca cgatctttct tctgtaacac tgaattttga aactccttcc 11880 aaagagacgc gacattttca aattcctttc caacaccgtt aatgctttca agatttttat 11940 ttagagctga cataagattc acaacttcat tcatactttt tgaaatttgt aaggtcaaaa 12000 gacgacgcct tctttcaaaa tctatatcat caaaatcttt attttgctca ttttgtatgt 12060 tttcagtaat atccatattt tctgaggaca tggtatgtta atggctagtg tcaaagtcaa 12120 gcatgagcca aagtttatca taaggttaca atcaagtaga tcttatatta gatttaacta 12180 tcttcataat taaatgccaa aattgaagct tccgtacgag catagaatat tagtcaaaag 12240 agacatgtta tgcatattta caagttggaa taaacaaata cattaaatat aataataata 12300 ataataataa taaatccgga tcagtactac aatgtaaata gaaaaggact aagcttcttt 12360 taaattactt atttcctgag cgcggcgtaa tctgcgttca agaatctcat cttccttaag 12420 taaagacctt tgtaaacgag cttcacatcc tacaacaggg catacacaag ttggatttaa 12480 cagagacaaa atagcatctt tttcataaaa atgattacag gctgtcgata atattgggtg 12540 aacaattggt tgtaaagtta aagggcatct gttatcaaag gtagctgaat ataccatcac 12600 ttcatctgct tcctgttctt cagtgttcat gacattaaaa aatgttttca tagaaggaaa 12660 gtcagaaccg tctgtatttt gctcatgcca tatggttttt ttaaattcaa tgtattctcc 12720 ttgtgtgcca tatttaccat agtcggattt ttcttcgtat tcttgaatga gttccttaaa 12780 aatttgtgta taaatatcca acaaactttt attctaattt tgttagaaca attgaatatg 12840 aaacagtaaa aaaatacgtc taaattgatg tttttacttc atctacatat aatagaaaaa 12900 gtaggaaagt catttccgtg gtacgtactt caaaatcact tatattttca ctggttttgt 12960 tagccaatgt atccaccgat tgctgcatca ataacaatct ctgctttgca tccaacagct 13020 ctcggatccc gctatcaagc atgtccaaac actaaaaggc aagtgttagt cagaagtcat 13080 aaagtaagca gcatgtcata ttatgtctat tttaggctct ctctcaatag ccggaaataa 13140 gaggaagaaa aatattagtt aaacaatgtt tttgtatgta tatttgtaca gcgaacttct 13200 ttttaaaaaa ctgtatctcg acataacacc actttcgagt tttaaaagca attcatatta 13260 attttttcca ttatctttcg taataatggt acacatacct cattattttt tatctcagca 13320 gcaactagag cacattttat cacctgttta attgaatcgt caatttcctt caattggaag 13380 tcgaaactgc aatgattttg gtttccaggt aataaatttt gacttaaagc ctccaaggaa 13440 gtttttaatt gtgcttcact catttacgat tccattcgag aaagaagtag ttacgacgtt 13500 taattagtag acaacgcaat ttgggattga aaacctatta attgcatatg aaaactaaat 13560 gccacaacac ttatttcctt aaataaaaaa agaatgtttt ttaagcaagg gtaataacca 13620 cgttttaaaa tttaaactcc agtacgtttt tatgaagatt gatggcatat gtataaagac 13680 taaataaatt cttatttact gtcacgttta atacaaatga atgggttatg aaatattctc 13740 atttggaatt agaatttata atgcatactt cggacaatta acgcttgact tttagtacct 13800 ctattactaa accaactatt caatatgata aaccaataaa ggatgattaa ggccgtgaaa 13860 tgtataaatt aaattatgtt attttcaaaa tattccatag attagtagta aagtaaaaca 13920 cgattaacaa aactatccga gtcttgtcaa tattattcat taaaaaagca atcatgaaca 13980 aactctatcc ggagacgaaa aattaagatt gataattaac aagctcttcc cgtaaaagtg 14040 gcggagttgc ctctatgtaa ggtattttat gaagttcaca ataggcctgg cccacagtaa 14100 catttttcca atttaatgac tttgaatata attcttgaag acgattctgc ttcataaacc 14160 ttgctttgat aagtctggcc ttgtctttta tctgtgttgg tccaaaagtt tttaacggac 14220 cattttcaag tttctgtatg tgaatgattt tagaccaaca acagccatgc tgagaaatca 14280 tttcgtaaag ctcattctcc tcttcatctg tccattttct acgagttcga tatccttcat 14340 atgggtttcc ttgcgacctg cgtgtacccg aacgagcagc agatctttca atggacactc 14400 tcattgccga acttctacca ccagatcgag attcttgctc gtctgattca cgtacaggcg 14460 gaaaatcttc acttaaatca tcctgttcac tactcaaaga agcttctctt ctcagattat 14520 cacccctctt cgcaatcagc ttactgcggt ttgcagcttc cttttgagaa agggacttag 14580 ctctgctttt tagagacttt gatagggtat gggagaaatc tctggcttga gaaaccgttt 14640 ctgataaatt atcagcactc aataagccag tgattttatc aagagcagat gaaactaaac 14700 ctaaggatac cacttctgat atttccgtat tatctattga gttcaaacct agggttcttt 14760 cagaaaaagt atcctcggac gcgtcactct catgaccact ggcttcgtct gctgactgtg 14820 cggtcgaacc agaaactcgc tcacgaagcc tgtttaagat ctgttggcta tcagagtcgt 14880 acagcatccc tttcacgcta aggtcctttc tttctaagcg acggacgttt ttaaattcta 14940 gtaaaaaatg tatgactgca ttgacaaacg ctggaaacga ggcccgtagc tgtagaggag 15000 aattagaagt attaccaatc gtagatttta gaaagtcaca aatcttttgg cttttccata 15060 cttgcccaca aactacgttt aaggcatcta aagatgtata aggaagacat gacaaaataa 15120 attcacttgc gtcaatagaa ggatttgaat cggtaccaaa gtatctaatc accaattcct 15180 caaatagaac caaggcatct ccataaaaag agtcttcttc atcagcagat aaaagtgcgt 15240 ctagtactat agatctcaaa gtaaggtgtt tgttggtatt atctaggaga tcaatcaagc 15300 ctgtaaaaaa ttgaatagat tttaaaggtt tttccaagtc ttcaaataaa caccaataag 15360 catcatattt tcgtatatag gagaaaaata tagtgcgaaa ctccagtcca tatttggtag 15420 tgagttgttc gttaaattca taaagatctt ttacagtttc cttccctaga cgttctgaga 15480 agctctcagg gttaacaata tcttttgaac tttcgctaaa aattgcagaa accatcatga 15540 ccttttgttc agccatgttt gctatatttt ggcaatcgtt aacgagcaaa gacgcctttt 15600 caatgtccca tatcgatcga actatcatag cattctggtc attttcactt tcccccgtcc 15660 ttgagtcttg tccctctgca gtcgagactt cgttttctac attcattgaa tggctaatta 15720 ctggaataga gatcgaacct tcagccatgt tttgagaatg ggaattaaaa caatatgccg 15780 gcttagaact ttcactctca ctagttgtaa aattcgataa attagatgaa ctctccgttt 15840 cagctctttc ctcaacagct ttagttaacg aatcagagat ttgacctgtt tcaatatttg 15900 agtctgagtt ttgtactacc tctttatcaa attgctggtc accttcattt tctaagactt 15960 tctgaatcgt ttctgtactt tgcacgctta tcatcaactg ccctcgattg tatggtgaaa 16020 gaggttgaat tacgaatttc actaaacaat tataaaatta tcataatccc ttagaacaat 16080 gtaagaatac catcgaacat ataatataat aaattaaaat tattcacttt tttttatgtt 16140 gctttgggtt tcaagtgttt cttagttttt cgcaagcgaa aagaaaggaa ctgtcggttt 16200 ttaatctttc cttatagatt ccatttctta ttgtagctat atttataaga tacacatcaa 16260 attttcaatt ttagcatacg taaaggataa aatacagatg ccggcaaaaa ctactgctat 16320 ctgactgacg cttaatggta tacgaagaaa ggttactgtt taatagaaaa aaaacaacgt 16380 cgtgtttact ctgcaataac agatgggcac caaatctttg aactaacaat aatacttttc 16440 attacctaaa atatgcttaa tattcaacaa gaacgtcaaa ctgaaacaag tttgttcaag 16500 cttctgtttt ctagaatttt tgttaattac ttggatcact tgtcttagga aatgaaacca 16560 ggcctcagta tgatttatgc gatagaaaat taaaacagta aagtgatgtg ttttattata 16620 acatgtaaat cattgaaatt gttgtcataa cacaaatgaa gtgaaccttg catagcttcg 16680 catgccttgt cgatttgcta tgctctaaag gatttataaa atacactctc tttaacgttt 16740 aattaaactc gtcattcatt ttatccaaaa tgtcttgatt cgcagataga gcacttatga 16800 ataatttctt cgcaaaatta aactgagaat gcagttcttt caactctttt ttcatcctcc 16860 taattatttt ttcatgttca gtattccgtt gcttttcttt tcgcagtaaa ttttctagtt 16920 cctgaatttg attagagtcc tattcgcttg gtcagttatg atc 16963 //