ID SPAC1635 standard; DNA; FUN; 2595 BP. XX AC AL356335; XX SV AL356335.1 XX DT 17-MAY-2000 (Rel. 63, Created) DT 17-MAY-2000 (Rel. 63, Last updated, Version 1) XX DE S.pombe chromosome I cosmid c1635. XX KW outer mitochondrial membrane; porin; KW voltage-dependent anion-selective channel. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-2595 RA Wood V., Rajandream M.A., Barrell B.G., Saunders D., Harris D.; RT ; RL Submitted (17-MAY-2000) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; Q9P544; Q9P544. DR SPTREMBL; Q9P544; Q9P544. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1635 is overlapped at the 3' end by cosmid c144, CC EMBL entry SPAC144, accession number AL132675. XX FH Key Location/Qualifiers FH FT source 1..2595 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1635" FT /map="IR" FT misc_feature 1..94 FT /note="nominal overlap with c30C2 S. pombe chromosome 1" FT repeat_region 353..374 FT /label={tg}11 FT /note="(tg)11" FT repeat_region 375..388 FT /label={t}14 FT /note="(t)14" FT repeat_region 389..399 FT /label={g}11 FT /note="(g)11" FT misc_feature 1487..2332 FT /note="Match to PF01459 Euk_porin, Eukaryotic porin Score FT 320.20" FT CDS 1487..2335 FT /db_xref="GOA:Q9P544" FT /db_xref="SPTREMBL:Q9P544" FT /label=SPAC1635.01 FT /note="SPAC1635.01, len:283, SIMILARITY:Neurospora crassa., FT PORI_NEUCR, outer mitochondrial membrane protein porin., FT (283 aa), fasta scores: opt: 813, E():0, (47.2% identity in FT 282 aa)" FT /gene="SPAC1635.01" FT /product="outer mitochondrial membrane protein porin; FT voltage-dependent anion-selective channel" FT /protein_id="CAB92123.1" FT /translation="MAPPAYAAINKLCNDLLQRDFPVGATLLSVRTTAPNGVVFNVSGN FT QDAKGVISGKLETSFNDKANGLTISQGWTTANVLESKVGLSEQFAPGLHLNVNTTFSPA FT TAAKTAILNLEHQHPLIHTHASVNALERKFLGDFTVGHEGFLAGAEFGYDVQKGNVSNY FT AATIGYLASPLSVALQASNNLSVFRASYYHRVSSDVEAGGNVTWDAASTANAITLELAS FT KYALDKDTFVKGKINSAGVATLSYFQTVRPGVTVGLGLQLDTQRLGQPAHKAGLSLAFS FT A" FT misc_feature 2495..2595 FT /note="nominal overlap with SPAC144 S. pombe chromosome 1" XX SQ Sequence 2595 BP; 742 A; 510 C; 493 G; 850 T; 0 other; atggaagttc tcatttagta agttgtataa atctcattaa gatgttttac agtaattctg 60 ctttgcattt cattccctta gcatcaggca gaaaacgttt gaggtaatct tcctctgaac 120 atttttcgac caacaaataa gcaaaggaat tggtccatag taatttaagc aagcaaaagt 180 gagtattgaa aacgagtcac tttgcagatg taaaaataaa ttcaactaat ccatgcgcga 240 agccaaacgc aattaagaag cctaggaatt ataaaaaagg gataccatca ttctgcaatt 300 gagacaaata aaaattcatt atttaattat caagattcgt gattaatatg tatgtgtgtg 360 tgtgtgtgtg tgtgtttttt ttttttttgg ggggggggga tatacactgg ctttacattc 420 agtaaaggct caattaattc ataatcacaa caagtttgag aaattgaaaa gagctaccgc 480 aaataagaca tgtataacag caagctggat tagaattcta attagtcgaa ccattgaaat 540 tcttttgatt actgaggttt tgaaaccata catttctctt aaacaccaaa catatgcaac 600 cttagtgtcg cactgcttat actaattttt aagattctca gtacgattag gttcagcgct 660 atatattctt atcaaggtat cctaaacgat taaaaagcat caccattgta aatgattaaa 720 aggaaataag gatcaattat tgtaagtagc gggtctgttt tcctcgatta gtctgtgaag 780 acattcgcta cagaaaaaga atgcacgtta acaaaagcac atgagttaag ttcctgttat 840 acaaatttga gaatattgaa gccaactaga ctttccgcta ctggatttgc aacttcaact 900 ttgccgattg tgaaaaaata gtgacgagca gcggacatct aaagcgtcgc ctgtcccgca 960 atgcgtagat gaaatccagt agatgcgagt aaaatagaaa agatggtgac atcgttactt 1020 caagatactg tgaaatagcg taagggtgtt gaaggaagac ttcattcggt tgcttcgcca 1080 tactaggtaa tacgtcggag tgccttgatg aaagtgcaac tttaattggt gaaaagtgcc 1140 aaagatggga attgcgaact caccatttac atcacacatt cattccgtta acttaccgtt 1200 ttcctgcttc ttgtttgaga aatttggaat ttgtaagtgc ttagaaattt gctgaattga 1260 ctgttcagca atatcttttc tttaaacaac tgctaacgtt tgtgctagtt tgatcgttag 1320 aagattcgcg aaaagtctat tgatttttct atccgtttga aaaaataccc aatacccgta 1380 cttagtttac cagctttttg aaattagcta gctaattatt ttacatttat ctgcttgttc 1440 tttttcctcg tgatctcatt atactatact cgattgatta gcaacaatgg ctccacctgc 1500 ttatgccgct attaacaaat tatgcaacga cttattgcaa cgcgatttcc ctgtcggagc 1560 taccttattg agcgttcgca ctaccgctcc caacggagtc gtcttcaatg tctctggtaa 1620 tcaggacgcc aagggtgtta tctctggtaa acttgaaacc agcttcaacg acaaggccaa 1680 tggcttgacc atctcacaag gatggactac tgccaacgtc cttgaatcca aggttggttt 1740 aagtgagcag ttcgctccag gattgcacct taacgtcaac acgactttta gccctgcaac 1800 tgccgccaag actgctattc tcaatttgga gcatcaacat cctttaatac atacacatgc 1860 ttctgtaaac gcgttggagc gcaagttcct tggtgacttt actgtcggcc acgagggatt 1920 cttggctggt gctgaatttg gttatgatgt tcaaaagggc aatgtttcta actatgctgc 1980 tactatcggt tacttggcta gtcctctttc tgttgccctt caagcttcca acaatctcag 2040 tgtattccgt gctagctatt atcaccgtgt cagctctgat gtagaagctg gtggtaatgt 2100 tacttgggat gctgcttcca ctgctaatgc tatcaccttg gagcttgctt ccaaatacgc 2160 ccttgacaag gatacttttg tgaagggaaa gatcaatagc gctggtgttg ctactttgtc 2220 ttatttccaa accgtccgtc ctggtgttac cgtcggcctt ggtcttcaac tcgacactca 2280 acgtttagga caacccgctc acaaggctgg cctttctctt gctttttcag cctaaaccag 2340 cttgtctagc catgatccca tcgtatgagc tgtttattaa tcctaccttc ctttaaatat 2400 gattgtaacg atttctcctc tttttcagaa gagtgatgtt ctgtcttgaa ttgagaacag 2460 cctgatgtca attgaaatgc tcgcttttta tgttgatcac attcccatag gtcttgtatc 2520 aatgaattat atttcttttt taattttaat ttaaacgtca tgtatatatt tgtgcaagtt 2580 aaatattttt tttta 2595 //