ID SPAC1327 standard; DNA; FUN; 2210 BP. XX AC AL035254; XX SV AL035254.1 XX DT 19-JAN-1999 (Rel. 58, Created) DT 22-DEC-1999 (Rel. 62, Last updated, Version 3) XX DE S.pombe chromosome I cosmid c1327. XX KW transcriptional regulator. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-2210 RA Wood V., Rajandream M.A., Barrell B.G., Harris D.; RT ; RL Submitted (18-JAN-1999) to the EMBL/GenBank/DDBJ databases. RL European Schizosaccharomyces genome sequencing project, Sanger Centre, The RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL barrell@sanger.ac.uk XX DR GOA; O94540; O94540. DR SPTREMBL; O94540; O94540. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced CC by the Sanger Centre. The sequencing of the S. pombe genome is now CC being continued with funding from The European Commission. CC Fourteen European sequencing laboratories, including the Sanger Centre, CC are participating in the project. CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPBC25H2.01c. CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1327 is overlapped at the 3' end by cosmid c18G6, CC EMBL entry SPAC18G6, accession number Z68198. Please note CC that this sequence does not represent the full insert of the cosmid XX FH Key Location/Qualifiers FH FT source 1..2210 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1327" FT /map="IL" FT misc_feature 1..140 FT /note="nominal overlap with cosmid SPAC1783 S. pombe FT chromosome 1" FT CDS complement(1..2210) FT /codon_start=2 FT /db_xref="GOA:O94540" FT /db_xref="SPTREMBL:O94540" FT /label=SPAC1327.01c FT /note="SPAC1327.01c, SIMILARITY:Schizosaccharomyc es pombe, FT O59746, hypothetical 90.8 kd protein., (819 aa), fasta FT scores: opt: 608, E():7.8e-26, (31.2% identity in 554 aa)" FT /partial FT /gene="SPAC1327.01c" FT /product="putative transcriptional regulator" FT /gene="SPAC18G6.16c" FT /gene="SPAC1783.09c" FT /protein_id="CAA22853.1" FT /translation="EPGTPKGYDGSKNSSPASVPSWIHEKTKAGKDRKRLPLACQSCRK FT KKVKCSGERPSCDQCLKHNIPCIYKSNSTKRSHSRHEEIHHQQQLHLNHQYQHQHKNVA FT ANSIDNLSRQYSQPNHINTHDSVNSPPSYGIMASATNQPLSHPTIVPSSNSSSLLNSTN FT NISHNPQVSLMSASLSKNLVLDPASPKSVSPDLVYVSSDNPPVSTAASAYSSSLPISDV FT TRALPLAPASNSQHPSLSSQPVSVPSNTINIPTDSLSIVSNPSQTPAKFDSNRISQVPN FT SDYSIYSNFNNFPIANHAPSFPQSSVKKLDSSFSPTSLPTFTTNSASSGLSTSYTNNND FT TTSDNNSLQAVPRLDPVPSLTLSSTPVAELPPLELRIHLAEVFFHCCHGQSYNLFHRPT FT FFESLNNNTVPLVVVYAVCAVSARFSSRMHDRFSPPYMAGEQYAREARRLALDNFDRPD FT LSLVAALLLLSLHDSGTCETGKSWMYGGMALRMAAALQLNCEQGSNPLDLDNIDSGPRI FT SFLERELRRRTFWSCFLMDRYASSHEHLQFLDENDIGIQLPVHELLFTKQIAGVTQTLD FT GRILEGVPSIVIPADTTENMGVAAYTVKIIALWGRAVKYLKQDGKRRDPYPYWHRNSDF FT SHISEALYAWADGLPQRLKYSAVGLENHLSIQQGAQYAFLHLAYHHTLMWLFRSIGETE FT NNQLSKISSSVSLAGNTVSFSPVSHTPINVTNGESQNNSNND" FT misc_feature complement(767..790) FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature complement(2009..2095) FT /note="PS00463 Fungal Zn(2)-Cys(6) binuclear cluster domain FT signature" FT misc_feature 2113..2210 FT /note="nominal overlap with cosmid SPAC18G6, EM:Z68198 FT S.pombe chromosome 1" XX SQ Sequence 2210 BP; 715 A; 367 C; 543 G; 585 T; 0 other; gatcgttatt gctattattt tgagattcac cattagtgac attaataggt gtgtgactca 60 ctggagaaaa agagactgtg tttcctgcta acgatacgga actagatatt ttggaaagtt 120 gattgttctc ggtttctcca atagaacgga ataaccacat taaagtgtga tgatatgcca 180 aatgtaaaaa tgcatactgg gcgccttgtt gtatagataa atggttttcc aatccaacag 240 cagaatattt aagccgttgg ggtaacccgt cagcccaggc atagagggct tcactaatgt 300 gagaaaaatc agaatttctg tgccaatatg ggtatggatc gcgtctttta ccatcttgtt 360 taaggtattt tacagcacgt ccccacaatg caataatctt cacagtgtag gcagcaactc 420 ccatattttc ggtagtatcc gcgggaataa caatacttgg gacgccttcc aaaattcgac 480 catcaagggt ttgcgtaacg ccagcaattt gtttagtaaa tagaagttca tgaacaggta 540 gttgaattcc tatatcattt tcatccaaaa attgtaaatg ctcatgggag gaagcatatc 600 tatccataag gaagcacgac cagaaagtac gacgcctaag ttcacgctcc agaaaagata 660 ttctgggtcc tgaatcaatg ttatcaaggt ccaaaggatt acttccttgt tcgcagttca 720 gttgtaaagc cgcagccata cgaagtgcca ttccaccata catccaactt ttgccagttt 780 cacacgttcc agagtcatgc aaggaaagca gtaagagagc agctaccaaa gataaatctg 840 gtctatcaaa attgtcgagt gctaaacgtc gtgcttcacg agcatattgt tcaccggcca 900 tataaggagg ggagaatcta tcgtgcattc gcgatgagaa acgggcggat acagcacaca 960 ctgcataaac aacgacaaga ggtacggtat tattatttaa agactcaaaa aaagttggac 1020 gatgaaacag attataagac tgaccatggc aacagtgaaa gaatacctct gcgaggtgaa 1080 ttcgaagttc caagggaggc aactcagcta cgggcgtgga agataaagtc agactcggca 1140 caggatccag acgaggaaca gcttgtaatg agttattatc acttgttgta tcattattgt 1200 ttgtataaga ggttgagaga cctgaggaag cggaattagt tgtaaatgta ggaagggatg 1260 taggcgaaaa tgaggaatca agcttcttga cagaagattg gggaaacgaa ggagcgtggt 1320 tggcaattgg aaaattatta aaattggaat aaatagagta atcagagttg ggcacttgag 1380 aaatcctatt tgaatcaaat tttgcgggag tttgagaagg attggaaaca attgaaagcg 1440 agtctgtcgg tatattaata gtattagaag gaacagaaac tggttgagag gaaagagaag 1500 gatgctgcga attagaggcg ggcgccaaag gcaaagcacg agtaacatcg gaaataggaa 1560 gcgaactaga ataagcagaa gcagcagtgg aaacgggggg attatcggag gagacataaa 1620 ccagatcggg agagacagat tttggagaag caggatctag gaccaaattt ttggagagag 1680 atgctgacat cagcgaaacc tgaggattat gagaaatatt gtttgtagaa ttaagaagag 1740 acgaagaatt tgaagaaggt actatagttg gatgggaaag aggctggttt gtagcagaag 1800 ccataatccc ataagaagga gggctgttaa cagaatcgtg cgtattaatg tgatttggtt 1860 gagaatattg ccgagaaaga ttatctattg aatttgcagc gacattcttg tgttggtgtt 1920 gatactgatg attgagatgt aactgttgct gatgatgaat ctcttcatgg cgtgagtgag 1980 aacgctttgt tgaatttgat ttgtatatac aaggaatatt atgttttaag cattgatcac 2040 acgaaggacg ctctcccgaa cacttaactt tcttctttcg acaagattga caggctaaag 2100 gtaaacgttt tcgatccttt ccagcctttg tcttttcgtg aatccaactt ggaacagaag 2160 caggagatga gttttttgaa ccatcataac cttttggagt tccaggctca 2210 //