ID SPAC1296 standard; DNA; FUN; 12580 BP. XX AC AL035439; XX SV AL035439.1 XX DT 24-FEB-1999 (Rel. 59, Created) DT 31-MAY-2002 (Rel. 72, Last updated, Version 5) XX DE S.pombe chromosome I cosmid c1296. XX KW cox4; cytochrome c oxidase polypeptide iv; mating response; oxidoreductase; KW phosphoacetylglucosamine mutase; serine carboxypeptidase; sxa2; tRNA Glu. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-12580 RA Wood V., Barrell B.G., Rajandream M.A., Harris D., Seeger K.; RT ; RL Submitted (12-FEB-1999) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: RL barrell@sanger.ac.uk XX DR GOA; O94611; O94611. DR GOA; O94612; O94612. DR GOA; O94613; O94613. DR GOA; P32825; P32825. DR GOA; P79010; P79010. DR GOA; Q09770; Q09770. DR SPTREMBL; O94612; O94612. DR SPTREMBL; O94613; O94613. DR SWISS-PROT; O94611; YFO4_SCHPO. DR SWISS-PROT; P32825; SXA2_SCHPO. DR SWISS-PROT; P79010; COX4_SCHPO. DR SWISS-PROT; Q09770; AGM2_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC Cosmid c1296 overlaps c22F3 at its 5' end. XX FH Key Location/Qualifiers FH FT source 1..12580 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c1296" FT /map="I" FT misc_feature complement(1..114) FT /note="nominal overlap with cosmid SPAC22F3, EM:Z54285 S. FT pombe chromosome 1" FT misc_feature complement(40..45) FT /note="gtaagt, splice donor sequence" FT CDS join(complement(963..1090),complement(812..897), FT complement(495..711),complement(<46..406)) FT /db_xref="GOA:Q09770" FT /db_xref="SWISS-PROT:Q09770" FT /note="SPAC1296.01c, len:276, SIMILARITY:Homo sapiens, FT G3851711, n-acetylglucosamine-phosphate mutase., (542 aa), FT fasta scores: opt: 519, E():8.9e-28, (39.0% identity in 241 FT aa)" FT /gene="SPAC1296.01c" FT /gene="SPAC22F3.01" FT /EC_number="5.4.2.3" FT /product="putative N-acetylglucosamine-phosphate mutase" FT /protein_id="CAB36507.2" FT /translation="MSDAEDSDSIVNEFVTAIVRESDKFAKVHSYPMQYGTGGYRADAE FT LLSSVAFRTGVIASFLSAKLHGQPVGLMVTASHNASSENGLKIVNILSSLDSSKWEAYL FT DQVVNADSADELTVCLTSILKKAKIIPGSEARVFVGYDSRSTSEILAQAVIDGIVVCKA FT KYENFGLLTTPQLHYMVKASQTYGTPDAIGEPTERGYFEKLSKAYQSLMTGKKIKGTVL FT IDAANGVGAAKIKELAKYIDPKLFPIEIVNDNIDNPELLNNS" FT misc_feature complement(407..424) FT /note="ctaatacttag, splice branch and acceptor" FT misc_feature complement(489..494) FT /note="gtttgt, splice donor sequence" FT misc_feature complement(712..724) FT /note="ctaacaatcttag, splice branch and acceptor" FT misc_feature complement(806..811) FT /note="gtaaga, splice donor sequence" FT misc_feature complement(898..915) FT /note="ttaacattttatttatag, splice branch and acceptor" FT misc_feature complement(957..962) FT /note="gtacgt, splice donor sequence" FT CDS join(2368..2479,2835..3217) FT /db_xref="GOA:P79010" FT /db_xref="SWISS-PROT:P79010" FT /note="SPAC1296.02, len:164" FT /gene="cox4" FT /gene="SPAC1296.02" FT /EC_number="1.9.3.1" FT /product="cytochrome c oxidase subunit IV" FT /protein_id="CAB36508.1" FT /translation="MFMNSMLRVSRQRAAVRSTVSLYRGFVSASIRRNEQNVVKAAAQE FT LANAKEPSDLIGPGGRDGEVPTDLEQATGLERYELLSELSGRDAFDMKPLDASRKGTLT FT DPIMVTSLDPYRHIGCTGSPSGSHNLIWMTVYKDKLRRCPECGSVYKLKFMGDPNAEHS FT H" FT misc_feature 2480..2485 FT /note="gtaagt, splice donor sequence" FT intron 2480..2834 FT /note="confirmed intron" FT misc_feature 2545..2844 FT /note="Pfam match to entry PF01215 COX5B, Cytochrome c oxid FT ase subunit Vb" FT misc_feature 2820..2834 FT /note="ctaacattcttttag, splice branch and acceptor" FT CDS complement(3558..5081) FT /db_xref="GOA:P32825" FT /db_xref="SWISS-PROT:P32825" FT /note="SPAC1296.03c, len:507" FT /gene="sxa2" FT /gene="SPAC1296.03c" FT /product="putative serine carboxypeptidase; involved in FT mating response" FT /protein_id="CAB36509.1" FT /translation="MLSLFLKSLFAIIIIELTIIHALPTYTVHWKCSIQQANTSSASSN FT QTVQPRQHAAPSSDRIKSLPEFKGSLPELYSGYLEANSDKSLFYTYAPAVVDSETFIVW FT LQGGPGCAGTLGFFSENGPIEISQSSPSPSLNPESWTNFANMLWLDQPFGTGYSQGQAA FT YTTTIEEASSDFVNALKSFYQKFPHLMKKKLYLVGESYGSIWSANFAEALLSEPSLNIN FT FMGVGIVSGLTADYETQEQITASIWVEHISKLGYYFNNTSSTISEEFKKRNKECQYDSV FT LNRLTFPTEQYPIWRPEYNFSTSTSLRKREALDGEDIGNVFNSISGCDLYSLSNFLLYL FT ENSCVITYDVSLDCSFNEYNDPLITYLNREDVRSSLHATKASTALTSGEGVFADGCNFD FT LYKKIVSNNVESVLVEIIPRLTEKYKVSFLAGALDLQILWTGTLLALQNTTWNGWQGFT FT QSPGSLETTNGFTLDERNLAFTLSNSVGHMAPSKDPQMVREWLENTLLY" FT misc_feature complement(3573..4889) FT /note="Pfam match to entry PF00450 serine_carbpept, Serine FT carboxypeptidase" FT 5'UTR 6444..6546 FT /note="mRNA from spc05626" FT CDS join(6547..6602,6650..6788,7061..7213,7290..7688) FT /db_xref="GOA:O94611" FT /db_xref="SWISS-PROT:O94611" FT /note="2 N-terminal exons added 28-5-2002" FT /gene="SPAC1296.04" FT /product="hypothetical protein; this *may* be the ortholog FT of S. cerevisiae SPO73; similarity is *very low* but they FT are reciprocal best hits" FT /protein_id="CAB36510.3" FT /translation="MTESERTSHLDILYENQRGIMLCGVPYFSEKSLLNFDPAPWVDQH FT FVASPVSTKTATCPDPSWEWAWQRWYIDMSGDVDESGWQYGFSFTMNNWHGKPVSLHSF FT VRRRRWIRKRKKKELRRDELPLVPESFTISSSYPIQRSSTQTRSSFEDDSSDFDLSEVT FT TIPALLRALQDSRIDREKLEALATFLASASIDEKVYVKNCKKDLLKNFVFEHSCRKANE FT LLNASLTAAEASTSQVALRESPTPQD" FT intron 6603..6649 FT /note="confirmed intron" FT intron 6789..7060 FT /note="confirmed intron" FT misc_feature 7214..7219 FT /note="gtataa, splice donor sequence" FT misc_feature 7274..7289 FT /note="ttaacgttttctatag, splice branch and acceptor" FT CDS join(complement(8741..8871),complement(8007..8652)) FT /db_xref="GOA:O94612" FT /db_xref="SPTREMBL:O94612" FT /note="SPAC1296.05c, len:258" FT /gene="SPAC1296.05c" FT /product="putative cyclin related protein" FT /protein_id="CAB36511.1" FT /translation="MADSLVHSLASSSQLEAFDSFEYAEELCTLGSEWIQEAGVLLNLT FT QNCVIVCLILFRRYCTLYPPRVPDLDAIVMACVSIGSKTTETPASVQDICNVVVYLKER FT FKDTNFEARGFIAHDLYSEEMYSSRNRLSNMELEVLRALNFDTHIVIPHKLAIHYLQTL FT QLIDNKKLLQITWNFLNDASRTRLCVLYPPFSLACGCIAMAARVIGMKLPKDWYRVFDT FT TKEEIDSLTSILENFYKTSAIAHKTLYLIFTEQVSA" FT misc_feature complement(8653..8665) FT /note="ttaacattgttag, splice branch and acceptor" FT misc_feature complement(8735..8740) FT /note="gtaagt, splice donor sequence" FT tRNA complement(9186..9257) FT /evidence=NOT_EXPERIMENTAL FT /note="tRNA Glu anticodon TTC" FT /gene="SPATRNAGLU.01" FT /product="tRNA, Glutamic acid" FT CDS join(10514..10648,10730..10807,10973..11091,11133..12477) FT /db_xref="GOA:O94613" FT /db_xref="SPTREMBL:O94613" FT /note="SPAC1296.06, len:558, SIMILARITY:Saccharomyces FT cerevisiae, Q12181, hypothetical 72.3 kD protein ypr044w., FT (623 aa), fasta scores: opt: 656, E():0, (32.5% identity in FT 631 aa)" FT /gene="SPAC1296.06" FT /product="putative NADPH cytochrome reductase" FT /EC_number="1.6.2.4" FT /protein_id="CAB36512.2" FT /translation="MKNSHIYILYGSETGTAEGLAESLFRSLTRMGYDVLVNSMDDFNL FT ENLLRPLQCVFICSTTGQGEMPLNMRERIITYRFNWASKKLDSRLRQLGAQSFSSRGEG FT DEQHPDGVEGVFAYWCNHLYSQLAAIKTPSRPAFGEFDLLPPSFQIIIDESLGCKVKGF FT EDNNIVRHSRGKIEATLVHNKRISNIKHWQDVRHLAFKIPNFERWKPGDVAVLYPWNDD FT MSVNSFIECMGWESIKYSPLIISSNVAERKLPWFPNILNVFNLVKYVLSIHSVPSRTFF FT EMASHFSNNKMHKERLQEFSSYKNIDDYYDYTTRPRRTVLETLQEFKSVQIPIEYALDA FT FPVIRGRQYSIANRCDNSTGILELAVALVKYQTILKSPRQGICSRWICDLHENTSFNID FT ILPGFLNLSYQSNKPLIMVGPGTGVAPLRALIQERIYNGLKENLLFFGCRNKSMDFLFE FT KDWEKYTEEGTLKLFCAFSRDQEKKKYVQHSIQENGELVYNLLNEKDGMFFVSGSSGKM FT PSSVKDAIAGIVSKYSGCSISDGYSFVTSLEKKNRYYQETW" FT misc_feature 10649..10654 FT /note="gtaagc, splice donor sequence" FT misc_feature 10717..10729 FT /note="ctaattattatag, splice branch and acceptor" FT misc_feature 10808..10813 FT /note="gttcgt, splice donor sequence" FT misc_feature 10956..10972 FT /note="gtaacttattttcaaag, splice branch and acceptor" FT misc_feature 11054..11710 FT /note="Pfam match to entry PF00667 FAD_binding, potential FT FAD binding domain" FT misc_feature 11092..11097 FT /note="gtaagt, splice donor sequence" FT misc_feature 11115..11132 FT /note="cttacttattttttgcag, splice branch and acceptor" FT misc_feature 11786..12121 FT /note="Pfam match to entry PF00175 oxidored_fad, FT Oxidoreductase FAD/NAD-binding domain" XX SQ Sequence 12580 BP; 4127 A; 2185 C; 2190 G; 4078 T; 0 other; agtaaattag catgcaattg taaacaatgt atcaaaaaaa cttaccgagt tattcaacag 60 ttcaggatta tcaatattgt cgtttacgat ttcgatagga aacagctttg gatcaatata 120 cttggccagt tcttttatct tagccgctcc gacgccgtta gcagcatcta tcaatacagt 180 ccctttaatc ttctttcctg tcattaagga ttgatatgcc ttcgacagct tttcaaaata 240 ccctctttca gtaggttcac ctattgcatc tggggtacca taagtttgag aagccttgac 300 catgtagtgt agctgaggtg tagttaaaag gccaaaattt tcgtactttg ctttgcagac 360 cacaataccg tcaattacag cttgcgcaag gatttctgaa gtagacctaa gtattagatg 420 ttagtcttgg acataagatt taaaaaagcg gagaaattaa gtgatctaat atcgacaata 480 aaagtaaaac aaaccttgag tcatagccaa caaacacccg agcttcagaa ccgggaataa 540 tctttgcctt tttcaaaatt gaagttaaac aaaccgttaa ttcgtcagcg gaatccgcat 600 ttactacttg atccaggtat gcttcccatt tgctagaatc caggctactc aaaatgttta 660 cgatctttaa cccgttttcg gaagaggcgt tgtgcgaggc agtcaccata actaagattg 720 ttagtaagta aactcaaaga aatacaaagg aacaagatgg atatcaatat gtaaatgaat 780 catctcgcaa taataaaagt atgtgtctta cgtccaacag gttgtccatg caatttggct 840 gacaaaaagc tggcgattac gcctgtgcga aaagcaacac tcgaaagcag ctcagcgcta 900 taaataaaat gttaataatt tccatttaaa ctaaaagtaa gtttcttttt ttatcaacgt 960 actcagcgcg atatcctcca gtaccatact gcatgggata agaatgcact ttggcaaact 1020 tatcggattc tcgaacaatt gccgttacaa attcatttac aatcgaatcg ctgtcttcag 1080 catcagacat agtggggaaa atatcgatac agaagtctga cgatcctttg taaaaaaaac 1140 ttgttaaagt atcgaaacta tgaatggaag aagaacgaag tcacttaagc ctaagcttcg 1200 agaaaacagg ttgacctcaa agctctctta ataaaaaagt acacttaaga agttaagaga 1260 tatgactttg tgttctcaag atatattcga gtcccgaata agcactggat tgctactaag 1320 ttgatgccaa acgcgtcaac tatcaccaaa ctatggcaat ttaaatatcg cggaataatg 1380 aactgcgtaa tacatattcg ttattagaag gaaatttggg aatagtttaa cgtctacgaa 1440 gttaataaaa gcttcacata tttcctcttt ctttattaca taaatattta tcaatttggt 1500 tttcatcgct tgggttatgt tcgaagttgc taaataaaca acttagttgg ttgactcaat 1560 agttaactct gtaaattagc tacaaaatag cagaaagaca cctcaacgtt ttttacaatt 1620 attatgaaaa gctatgaaat ttaatgtgaa taaactagat gaaaagaaac gactaaatga 1680 accaacgaaa aggtattaaa aaatagtatt tcgtattaga tttaattgat taacacaatg 1740 tggtcagtaa tatacagaga atgtatgcat gtttgcttta aacaatagaa caaacggatt 1800 gtaataattg agaggacaag atcgaaaatg aacgacctga aaatcgttca tcactaggga 1860 atttatttct ttcggtcata ttgcttttaa aataattaat attcccgagg caaacatgat 1920 acattaaacc tctagttcat aaagagttgt ataagaaatg agtaaacaaa acaatttaag 1980 tcaatttctt ccagggtata gctcatgaac cttaaaaaac acaaacaatc agtagaaata 2040 tagcaatgat ccctttaatt ataacactca gatatgttta gcaagttatg agcaccgata 2100 gtcaatcaag aaaaggacat ctcatactac tcgtttttta tacaatataa ttaaggcaat 2160 agtaatattg gaacttcccc attatcatct tattcgtaac tgtttaagaa ataattccgt 2220 tttttgttaa ccttctcatc tttttttctg ttgtagcttg ctgtcctacc aaatttcgtt 2280 acacaatacc ggaccaacgt tggaaactac acaacacccc atctcaccca ttatcaggtt 2340 ataaatcatc aataattgtc ttttaagatg tttatgaatt ctatgcttcg tgtctccaga 2400 caaagagcag cggtcaggag tactgtttct ctgtaccgtg gctttgtttc tgcttcaatc 2460 cgccgtaatg agcaaaacgg taagtccttt gaaagcttag taaagtttgt catagcagct 2520 taatggttaa agcttgttta gtactggcta taggagtaaa gcttccgttg actcttgatc 2580 tccgatgtac agttttgcta ttaattatat atcggaagtt aggcttgtgt gcccattcat 2640 tcttttctat attttacaga cctaaaaagt aatcaaaaga atgaaaatcg acgttgccta 2700 tagcccactc tcttaaaacg ctttctattt catctattaa gtcgcttagt agatatgact 2760 tgttaatttg tgctgccgat acattttagg gctttttagc ttaattttta tctccttcac 2820 taacattctt ttagttgtaa aagccgccgc tcaagaatta gccaacgcta aggaaccttc 2880 tgatttgatc ggacctggtg gtcgtgacgg cgaggttcca actgatttgg agcaagccac 2940 tggtttggaa cgatatgaac ttttatcaga gctttcaggg agagatgcat ttgatatgaa 3000 accgttggat gcttctcgta aaggcacatt gacggatcct ataatggtca cttctcttga 3060 tccttaccgt catattggct gtactggtag tccttctggt tcacataatt taatttggat 3120 gactgtctac aaagacaaac tccgccgttg tcctgaatgc ggatctgtat acaaattaaa 3180 attcatggga gatcccaacg ctgaacacag tcattagtat gcttaccaat cttccatatc 3240 cccttgctat tttcatactc ttttataaca atttagctct tttatttgaa ctttgaaaaa 3300 tcaatgaaaa ctgtatgctg atctatttct tattttggga acgattcgat tattcgtaaa 3360 cgtatatctt ataaataatt ctatctattc aaccatatct tagttgttaa ttcaagcacc 3420 attctttttt tcttgtagtc atgcggaaat atgatctaat tatcgttccg agtttcataa 3480 acgatagtta tttattgata taccacatca gtggtaagtc aataaacttg tattttaaat 3540 tttccgatat taaactttta ataaagcaac gtattttcaa gccattctct aaccatttga 3600 gggtctttag atggagccat gtgcccaacc gagttggaca atgtaaaggc taagttacgt 3660 tcatccaaag taaatccatt agttgtctct aaacttccag gagattgagt gaatccctgc 3720 catccattcc atgttgtatt ttgcaatgcc aaaagagttc cggtccaaag aatttgaaga 3780 tccaatgccc cagctaagaa agacactttg tatttttctg ttaagcgtgg aattatttca 3840 acgagaactg attctacgtt gttactcaca atctttttgt ataagtcaaa attgcatccg 3900 tctgcgaaga caccctcgcc gcttgtcaac gcagttgaag ctttcgtagc atgtaaagaa 3960 gagcgaacat cttctctgtt caggtatgtg attaagggat cattatactc attgaaacta 4020 caatcaaggc taacatcata agttatgaca cagctgttct ctagataaag taaaaagtta 4080 gaaagggaat acaagtcaca tccagaaatt gaattaaata cattcccaat gtcttcacca 4140 tcgagagctt cccgcttcct tagagaagta gatgtgctaa agttgtactc tggtctccat 4200 attgggtatt gttcagtagg aaacgtcaat ctgttcaaaa ctgaatcata ttgacattct 4260 ttattgcgct ttttaaactc ttcagatatt gtggaagaag tattattaaa atagtatcca 4320 agtttgctga tatgttccac ccaaatagaa gcagtaattt gttcttgagt ttcgtagtcc 4380 gccgtaagtc cactgacgat tcccacgccc ataaaattta tattcaaaga tggttcagaa 4440 agtaatgcct ctgcaaaatt ggcgctccat atgctaccgt agctttcgcc gacaaggtag 4500 agctttttct tcattaagtg gggaaacttt tggtaaaatg atttcagggc atttacaaaa 4560 tctgaagaag cttcttcaat cgtagttgta tatgccgcct gtccttggga gtaccccgta 4620 ccaaatggct gatctaacca taacatgttg gcgaagtttg tccaagattc aggattaagg 4680 gatggtgagg ggctactttg ggatatttca atgggtccat tttcactgaa aaagcctaat 4740 gttccagcgc atccaggtcc tccttgaagc catactatga aagtttccga atcaacaaca 4800 gcaggggcat atgtataaaa aagtgattta tcggaattcg cttccaagta tccagaatat 4860 aattccggta atgatccctt aaattcagga agtgatttaa ttcgatccga agaaggagca 4920 gcgtgttgtc taggttgaac agtctgattc gaggaagcag aactcgtgtt ggcttgttgt 4980 atactgcatt tccaatgtac agtgtaagtt ggtaaagcgt gaatgatagt caattcaatt 5040 attataatag caaaaagaga tttcaaaaat aaagacagca ttgaaaagag agacaatgat 5100 tttgagccta tgagaaaagc gttagcgcta aattaaatat aatatttatc atatttggaa 5160 tatattgatg tattcagttt cgaaacaatt cttgaaaagt gactataatt ttatacgctt 5220 cagtatttcg aaaaatgtga aaatgctatg acttactcga ataaagaaac cggattgttt 5280 atctgattca ttttcataag agttgcatgc tatatatgat aatacataat aatggggatt 5340 cacttgaata gattagcatt gtccattgtt tacaatcaac aacaatagag atgggcaaca 5400 aagacaccca gcgaagtacc atttactagt aataggctaa ttcatttaag tttttcaaca 5460 caatagtcct taatgaaaaa accaattgtt tcgtttacgc ggcaagtgaa tagggtagga 5520 ctagaataac ctatggtctg tttaaatcga aaaccagctt atttcagcag cgatcattta 5580 tgcattctta atgcttaaca atgcgaggcg actcaaagaa tggagaaaat tgattgtttt 5640 atgaccggtg gaatcgaata atttgacttt tgctatgaag gctttagccc ttagaaataa 5700 agaaataaaa taaaaaatag atagataaca agaaaaaatc aatgtcaaat aatttagttg 5760 tattaatgaa aaataagaaa taactaaatt aaaaaaatat aaatacattt atatattagt 5820 aacactattt ctttttaaag accttgaaat attatgtgag aaacttaaaa actaataaaa 5880 ataaataaat ataaaaatat aaagaaaaag aaaaaaatta aaaattatat atacaatata 5940 ctaataaaca tagtgattaa ttaaatgaaa aaaataaatg tttcttaaag catggttaaa 6000 acacacaatg gcggatttct agccatgtag ctcaattttt ctgttgatcc gttcttgaat 6060 tatagtatag aaattattgc aattgcatga aaaaacttat catgaaactt ttttggtaaa 6120 aagagcttca gtttgttgtt ttcatgttcg aaagacagtt tctattaata tttatatccg 6180 tcctagaatg aatagtaact tggtgcacca aaaaaaacat tgcaaacaaa cacagctgta 6240 ttctgtctgc aacttgcttt tatggggaag ttttttatat aaaacatttt gttgtttact 6300 taacaatata ctttaaccaa atattattct ctaaattgtt tagtagcttg ttgcgtcatt 6360 tagctagtaa acaccaaaaa tcggctgttg tcaaaacggc cgccgttttg cacatacaat 6420 aaagaaccca ctaccccata atctttataa taaacagctt ttaattaatt tcttttttaa 6480 tatagatttt atttcctata atttctctct taatttctag atggaactaa ataagatcct 6540 gcatagatga cggaatctga aaggacttca catcttgata ttctctacga aaatcaacgt 6600 gggtatgaca aagcaatttt gcagtttgaa actaaacatt tatacttagt attatgctat 6660 gtggggtacc ctatttctct gaaaaatcat tgttgaattt tgaccctgct ccttgggttg 6720 atcagcattt tgttgcgtct cctgtctcaa cgaaaacagc aacttgtcct gatcctagtt 6780 gggaatgggt atgtcaataa cttcttctct gtcttcctct aggacgatag cgataagacc 6840 agcatatagt ttgcttgaaa actgtttgct aacaatatac tgcttttgtt gtaacatttt 6900 cctctctgaa tttaagaaag ctatttgttt tacaccaatg aaattagctg tagttgaacc 6960 atcctttggc tcaattattt aagtttcatt tggttctttg cttttggtta gctaaatttg 7020 tgaatggatg tgttaccaaa aaagcagttt aaatgtatag gcatggcagc gttggtatat 7080 tgacatgtcc ggcgatgtcg atgaatcagg ttggcagtat gggttttcat ttaccatgaa 7140 caattggcat ggtaaaccgg tttctcttca ttcgtttgtt cgtcgcagac gatggatccg 7200 gaagcgaaaa aaggtataat acatcacagg ctactttaat tgtgctagtt tgaaaattcg 7260 ttccgttcat attttaacgt tttctataga aagaattgcg acgagatgaa cttcctcttg 7320 ttccagagtc cttcacaatc tcctcttcat atcccataca aaggtcaagt actcaaacac 7380 gaagcagctt tgaagatgat tcatcggact ttgatctttc agaggttacc accattcccg 7440 cccttttacg cgctcttcag gatagtcgaa ttgaccgtga aaaattggaa gccctagcaa 7500 cctttttggc ttccgccagt atagatgaaa aggtttatgt aaaaaactgc aaaaaggacc 7560 ttctcaaaaa ctttgttttt gaacatagtt gccgtaaagc taatgaactc ctaaatgcgt 7620 cgctgactgc ggcagaagca tctacctccc aagtggcttt acgagaatct ccaactcccc 7680 aggattgaac aagataatca gcccactaat ttctgactgt atccatttca attagtttat 7740 tatcgtcttt cctcgaatgc tttcctctat aaacgaaaag gatctgaaag atgtgatacg 7800 ttcttaaaga tacacacgtg tttttttaat ggttgtttat gctttattta tagccaagtt 7860 tgcaaaattg atgtcgctaa cttgtatctt aacaattcgc tcactactac tacagaaagg 7920 tatatacaat taatgaatac tctcgttttt taaataagga tatatttgtg ttccattctg 7980 caatgttaaa caggaagatg tacactttaa gcagaaactt gctcagtaaa aatgagatac 8040 aatgttttat gagcaatagc gcttgtttta taaaaatttt ccaaaatgct tgttaaagaa 8100 tcgatttctt cttttgtagt atcaaagact cgataccagt ctttgggtaa tttcataccg 8160 ataactctag ctgccattgc gatgcagccg catgccaaac taaacggagg atacaaaacg 8220 caaagtcgag ttcggctagc atcgtttaaa aaattccatg ttatttgtaa aagctttttg 8280 ttatcaatta gctgtaatgt ttgtagataa tgaattgcca gcttgtgagg aatgacaata 8340 tgggtatcaa aattcaatgc tcgtaagacc tccaattcca tgtttgataa tctattcctg 8400 gaactataca tctcctcact gtacaaatcg tgagcaatga atcctcgtgc ttcaaaattc 8460 gtgtccttga aacgctcttt aagatagact acaacattac atatgtcttg tacagaagct 8520 ggagtttcgg tagttttact tccaatggac acacaagcca taacgatagc atcaagatcg 8580 ggaactctcg gcggataaag tgtgcagtat cttcgaaaca aaatcaaaca aacaattaca 8640 caattttgag ttctaacaat gttaataaaa agttcaaatg cattttatcg ccacaatccg 8700 gctactaaaa gctttggaaa ataaatgtaa gaagacttac agattcaaaa gaacgcctgc 8760 ttcctgaatc cattcagaac ctaatgtaca tagttcctct gcatattcaa aagaatcaaa 8820 ggcttctaac tgggaagaag aggcaaggga atgtaccaaa gaatctgcca tcagaatatt 8880 cctcaagaat cagaattatt tacttaaccg aaaacacgat attcccatac cccgttcatg 8940 acctgcgttt ttgcataaga atacggtggg agtttcgctg agcgattgtt gtcacttaga 9000 gcaaattatt gctacttgta gcaaattgca ccattaaata aaaggttcgc tttgaattga 9060 atttcgtagt tcaggatcaa ataaaatctt ctaaattctc acaatacaat gaacgtaact 9120 tcttcaaaac attaattttg cttttaattc aaaaagtgat ttactctagt agtagtaaaa 9180 atttactccg ttgcggggag tcgaaccccg accgcgtcgg tgaaagcgac gaatcctagc 9240 cgttggacca caacggaacc gttgattgct tgttgcacaa tgtaatatat gtacagtaaa 9300 attttgttaa ttaaaaatta aattgttaaa atttagtaga aaatagtact ttttctggta 9360 agctactggc aatcttaaat atcggtcgag agtataaata gaagtgtcga ttgctacatt 9420 aaataaaaac ttaaaattta ctttagtgta tgcgacaatg aatgaccttg ttgtgattgt 9480 ctggaaacaa ttctggttat ttattgttat tattattcgt gattgttgag attggacctt 9540 aagtttgaat tttagaacat tgtaaataaa tttcctattt ttggatatat tttaaatcta 9600 aatctcgttt aactgagtaa attcatatca agaactcact gacaagcctt taaatctaat 9660 tttctaatct tttacatgtt ttttatagac aaaaaaaacc tattatatgt tagtggtaac 9720 taaatgttaa agaagtaact caataccata tagcaattca gatagggcct tttgcaatat 9780 gtaaacttca atggtgcgag tacatttttg ctatacaaat ctctattgta ttctctcgta 9840 caatcgttag catctgaatt tttaaacttg ttgtacaaat attcaatatg ttcagttgca 9900 tgggatcgac attgagttta ctatcttatg tgatctgata aattaagctg tcgttactga 9960 aagagcttac tacctggaaa gtcataaaca atgcgccatt taatactctc accataagcg 10020 tgtttgatca aagcactcgg caatctaatg gaactcagcg ttgttgcttt aacttaggat 10080 tcggctgttg gatctaaatt gtctcgaagg aattactaaa agttcaataa acaaaagtat 10140 ttgataagca gtattttact tattaccatc gctcttaaaa agataaacta cgcatattta 10200 tatttaacgt ctaccgccaa aaagaaatct tgtaaagcaa agacctctta agtttgttag 10260 tttttcatta aattattacc cactttggca atagtcaaaa tcaacaaatt gttttaccat 10320 caaaattaat aaagtaaacg tttcttaaat ttaagaaaat taattggtag cgttaaggaa 10380 ttcctatgtt ggctttaagt tggcataatg ctttacacta tcgcactact cgtcttgcac 10440 gtacctttat caaactgagc gcaaggtgcc gcattccctt ctgcgccaaa atttcaatcc 10500 aaacttaaga aaaatgaaaa attcacacat atacatatta tatggttccg aaactggaac 10560 cgctgagggt ttagcggaat cactatttcg tagtcttaca cgaatgggtt acgatgtgtt 10620 ggtaaattcg atggatgatt tcaacttagt aagcatagta gctgtatata tatatatata 10680 tatatatgta tctataataa tgaaagtttg atctaactaa ttattatagg aaaatttgtt 10740 gcgtccactt caatgtgtat ttatttgcag tacaactggt caaggggaga tgccccttaa 10800 tatgagggtt cgtgaataaa aattttcttt tgtctttaga tactaatgtg agagcagaaa 10860 ttttggagat tcttactaag aaaaaaacta cccaacactt tcttaaatga tatgcaatat 10920 gcggtgtttg gttgcggtga cacttcatat acgaggtaac ttattttcaa aggagagaat 10980 aataacatat agattcaatt gggcttccaa aaaattggat agtcggttgc gccagttggg 11040 tgcacaatcg tttagttcgc gtggtgaggg tgatgaacag catcctgatg ggtaagttaa 11100 tgataataag tattcttact tattttttgc agtgttgaag gtgtttttgc atattggtgc 11160 aatcacttat attctcaact ggctgccata aaaaccccct ctcgtccagc cttcggagaa 11220 ttcgatttgc taccgccttc gttccaaatt attatagacg aaagtttagg ttgcaaagta 11280 aaaggatttg aagataataa tattgttcgc cattctcgtg ggaaaattga agcaactctt 11340 gtacataata aacggataag taatataaag cattggcaag atgttcgtca tctcgctttt 11400 aaaattccaa actttgaaag gtggaagcca ggagacgttg ctgttcttta tccttggaat 11460 gatgatatga gcgttaactc gttcattgaa tgcatgggtt gggaatccat aaaatactct 11520 cctttgatta tttcttccaa cgttgccgaa cgcaagcttc cctggtttcc aaatattttg 11580 aacgttttta atcttgtgaa atacgtcctt tcaatccatt cagtaccatc aagaacattc 11640 tttgaaatgg catctcactt ctcaaataat aagatgcata aagaacgtct tcaagaattt 11700 tcttcttaca aaaacattga tgattattat gattacacaa ctaggcctag aaggactgtt 11760 ttggaaactc ttcaggaatt caaatctgtc caaataccta tcgagtatgc attagatgcc 11820 tttccagtta ttcgtggacg tcaatattct atagcaaata gatgtgataa ttctactgga 11880 attttggaat tggcggtagc gctggtgaaa tatcaaacta tattgaaaag tcctcgacaa 11940 ggtatatgca gtcgttggat atgtgattta cacgaaaata cctcttttaa tattgatatt 12000 cttcccggat ttctaaatct atcttaccaa agtaataagc cattaattat ggttggccca 12060 ggaacaggag ttgcaccttt aagagcatta attcaagaac gaatatataa tggcttaaaa 12120 gaaaatcttc tgttttttgg atgtagaaac aaatctatgg attttctttt tgaaaaggat 12180 tgggaaaaat acaccgaaga aggcacactg aaattgtttt gtgctttctc acgcgatcaa 12240 gaaaaaaaga aatacgtgca acactcaatt caagaaaatg gcgagttagt atataattta 12300 ttaaatgaga aagatggcat gttctttgta agtggatctt cgggcaaaat gcctagttct 12360 gtgaaagacg cgattgctgg cattgtttct aaatattcgg gctgcagtat atctgatggt 12420 tatagttttg tcacttcatt ggaaaagaag aatagatatt accaggaaac ctggtaaata 12480 atgcaatgaa ataccttacg tactaaagtt atttttgata tattgcttga aagacacagc 12540 catgaatatc ttgctgctta gtacttgacc agtaaagatc 12580 //