ID SPAC11G7 standard; DNA; FUN; 15885 BP. XX AC Z99161; XX SV Z99161.1 XX DT 16-SEP-1997 (Rel. 52, Created) DT 07-MAR-2000 (Rel. 63, Last updated, Version 3) XX DE S.pombe chromosome I cosmid c11G7. XX KW isocitrate dehydrogenase; malony coa-acyl carrier protein; pub1; KW serine rich; ubiquitin; ubiquitin ligase. XX OS Schizosaccharomyces pombe (fission yeast) OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. XX RN [1] RP 1-15885 RA McLean J., Harris D., Barrell B.G., Rajandream M.A., Wood V.; RT ; RL Submitted (13-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL Schizosaccharomyces pombe chromosome I sequencing project, Sanger Centre, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: RL barrell@sanger.ac.uk XX DR GOA; O13695; O13695. DR GOA; O13696; O13696. DR GOA; O13697; O13697. DR GOA; O13698; O13698. DR GOA; Q92462; Q92462. DR SPTREMBL; O13697; O13697. DR SPTREMBL; O13698; O13698. DR SPTREMBL; O13699; O13699. DR SWISS-PROT; O13695; YEN1_SCHPO. DR SWISS-PROT; O13696; IDH1_SCHPO. DR SWISS-PROT; Q92462; PUB1_SCHPO. XX CC Notes: CC Details of yeast sequencing at the Sanger Centre are available on CC the World Wide Web. CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CC Protein coding regions (CDS) have been predicted with the help CC of computer analysis using the Genefinder program in PomBase CC (an ACEDB database) with additional predictions for the CC branch-acceptor sites supplied by the program Sp3splice. CC CAUTION: It is possible that for any individual CDS we may have CC underestimated or overestimated the number of introns/exons or CC we may not have chosen the correct splice donor/acceptor sites. CC CDS are numbered using the following system eg SPAC5H10.01c. CC SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), CC .01 (first CDS), c (complementary strand). CC The more significant matches with motifs in the PROSITE CC database are also included but some of these may be fortuitous. CC The length in codons is given for each CDS. CC IMPORTANT: This sequence MAY NOT be the entire insert of CC the sequenced clone. It may be shorter because we only CC sequence overlapping sections once, or longer, because we CC arrange for a small overlap between neighbouring submissions. CC The 5' end of 11G7 overlaps c2F3. XX FH Key Location/Qualifiers FH FT source 1..15885 FT /chromosome="I" FT /db_xref="taxon:4896" FT /organism="Schizosaccharomyces pombe" FT /strain="972h-" FT /clone="cosmid c11G7" FT /map="IR" FT misc_feature 1..238 FT /note="CDS completely contained within SPAC2F3.16, unknown" FT misc_feature 1..1444 FT /note="nominal overlap with cosmid SPAC2F3, Z99165 S.pombe FT chromosome 1" FT CDS 1533..3143 FT /db_xref="GOA:O13695" FT /db_xref="SWISS-PROT:O13695" FT /label=SPAC11G7.01 FT /note="SPAC11G7.01, len:536, SIMILARITY:Schizosaccharomyces FT pombe, CAA22127, serine-rich protein., (534 aa), fasta FT scores: opt: 486, E():8.8e-14, (47.7% identity in 239 aa)" FT /gene="SPAC11G7.01" FT /product="hypothetical serine-rich protein." FT /protein_id="CAB16206.1" FT /translation="MSFTNNTSSVDTSLSSSASSSIPASSSSAAASTSLSSSSVIPSSS FT SSMLSSSSATAISSSSSSSPLSSSSFTSPASSSFITSLVSSSSQQSSSSSASLTSSSSA FT TLTSSSSASPTSSSSSHALSSSSSSLVASSSSSGMSSSSLSHSSSVPSSSSSYHSSSMT FT TSGLSSSASIVSSTYRDGPSIITLVSTSYVSEVVTPTTTNNWNSSSSFTSSTSSTPISS FT SYSSSGTLPSKSNKSSNHVGVVVGCSVAIPVGVVLILIGLGIFLWKRHQRSKRIKAERM FT QEVEEYGFNPNQPSNFRSPNRAPSTNNRYRGWNGSPTPAAGNNTNGRPVAPRPSAGAGG FT ANPPAASQPGLLGGSSNSAGPIAAATAAGVGADASDAANTGGSFTRPQGARMVRPIGNP FT PDLSASNEAEATMPPSNGSNFSEGLSASPFESGPAVGAAGAAAEAAEHSGSGSDSYPEG FT PLATIPESDSESMASDLAGESSYGSRAALSSRSQSNLLSPTSTGASNQPNYSPFADNPS FT SSNVSIPRSSSEARRLNLF" FT CDS join(4958..4974,5026..5048,5100..5244,5387..7505) FT /db_xref="GOA:Q92462" FT /db_xref="SWISS-PROT:Q92462" FT /label=pub1 FT /note="SPAC11G7.02, len:767" FT /gene="pub1" FT /gene="SPAC11G7.02" FT /product="ubiquitin--protein ligase pub1 (EC 6.3.2.-)" FT /protein_id="CAB16207.1" FT /translation="MSNSAQSRRIRVTIVAADGLYKRDVFRFPDPFAVLTVDGEQTHTT FT TAIKKTLNPYWNETFEVNVTDNSTIAIQVFDQKKFKKKGQGFLGVINLRVGDVLDLAIG FT GDEMLTRDLKKSNENTVVHGKIIINLSTTAQSTLQVPSSAASGARTQRTSITNDPQSSQ FT SSSVSRNPASSRAGSPTRDNAPAASPASSEPRTFSSFEDQYGRLPPGWERRTDNLGRTY FT YVDHNTRSTTWIRPNLSSVAGAAAAELHSSASSANVTEGVQPSSSNAARRTEASVLTSN FT ATTAGSGELPPGWEQRYTPEGRPYFVDHNTRTTTWVDPRRQQYIRSYGGPNNATIQQQP FT VSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKLIY FT FLSQPALHPLPGQCHIKVRRNHIFEDSYAEIMRQSATDLKKRLMIKFDGEDGLDYGGLS FT REYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGINPEHLNYFKFIGRVIGLAIFHR FT RFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITGVLDLTFSVEDNCF FT GEVVTIDLKPNGRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSELIPQELIN FT VFDERELELLIGGISEIDMEDWKKHTDYRSYSENDQIIKWFWELMDEWSNEKKSRLLQF FT TTGTSRIPVNGFKDLQGSDGPRKFTIEKAGEPNKLPKAHTCFNRLDLPPYTSKKDLDHK FT LSIAVEETIGFGQE" FT misc_feature 4975..4980 FT /note="gtaagt, splice donor sequence" FT intron 4975..5025 FT /note="confirmed intron" FT misc_feature 4985..5233 FT /note="Pfam match to entry PF00168 C2, C2 domain," FT misc_feature 5009..5025 FT /note="ctaacttttatgattag, splice branch and acceptor" FT misc_feature 5049..5054 FT /note="gtatgt, splice donor sequence" FT intron 5049..5099 FT /note="confirmed intron" FT misc_feature 5086..5099 FT /note="ctgacccaaaatag, splice branch and acceptor" FT misc_feature join(5099..5244,5387..5477) FT /note="Match to PF00168 C2, C2 domain Score 57.45" FT misc_feature 5108..5155 FT /note="PS00499 C2 domain signature" FT misc_feature 5245..5250 FT /note="gtaagt, splice donor sequence" FT intron 5245..5386 FT /note="confirmed intron" FT misc_feature 5372..5386 FT /note="ctaacaattctttag, splice branch and acceptor" FT misc_feature 5820..5909 FT /note="Match to PF00397 WW_rsp5_WWP, WW domain Score 60.42" FT misc_feature 5825..5914 FT /note="Pfam match to entry PF00397 WW_rsp5_WWP, WW domain," FT misc_feature 5832..5909 FT /note="PS01159 WW/rsp5/WWP domain signature" FT misc_feature 6069..6158 FT /note="Match to PF00397 WW_rsp5_WWP, WW domain Score 62.17" FT misc_feature 6081..6158 FT /note="PS01159 WW/rsp5/WWP domain signature" FT misc_feature 6240..6329 FT /note="Match to PF00397 WW_rsp5_WWP, WW domain Score 51.71" FT misc_feature 6252..6329 FT /note="PS01159 WW/rsp5/WWP domain signature" FT misc_feature 6588..7502 FT /note="Match to PF00632 HECT, HECT-domain FT (ubiquitin-transferase). Score 602.97" FT CDS 8470..9540 FT /db_xref="GOA:O13696" FT /db_xref="SWISS-PROT:O13696" FT /label=SPAC11G7.03 FT /note="SPAC11G7.03, len:356, SIMILARITY:Ajellomyces FT capsulatus., O13302, nad(+)-isocitrate dehydrogenase FT subunit i precursor., (388 aa), fasta scores: opt: 1641, FT E():0, (70.4% identity in 348 aa)" FT /gene="SPAC11G7.03" FT /product="putative isocitrate dehydrogenase (EC 1.1.1.41)" FT /protein_id="CAB16208.1" FT /translation="MFKSLVRKSSAFQPLKYGGKYTVTLIPGDGIGRETSNAVTEIFKT FT ANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSLKRNKVGLKGILFTPFEKGGHTSFNVA FT LRKELDIYASLVLIKNIPGFKTRHDNVDFAIIRENTEGEYSGLEHQSVPGVVESLKIIT FT EYKSKRIAQFAFDFALQNGRKSVTCIHKANIMKLADGLFRRTFYDVANGYDAITPKDLI FT VDNASMQAVSRPQQFDVLVMPNLYGSILSNIGSALVGGPGVIPGANFGRDYALFEPGCR FT HVGLSITGRGEANPTAAILSACLMLRHLGLKDYADLINAATYSVIEEGKTLTKDLGGSA FT STGDFTHAILERMESL" FT misc_feature 8533..9516 FT /note="Match to PF00180 isodh, Isocitrate and FT isopropylmalate dehydrogenases Score 321.14" FT misc_feature 10644..10871 FT /note="Match to PF00240 ubiquitin, Ubiquitin family Score FT 180.83" FT CDS 10644..11030 FT /db_xref="GOA:O13697" FT /db_xref="SPTREMBL:O13697" FT /label=ubi1 FT /note="SPAC11G7.04, len:128, SIMILARITY: eg. Magnaporthe FT Grisea (rice blast fungi), O59962 , ubiquitin fusion FT protein, (128aa), fasta scores: opt: 834, E(): 0, (97.7% FT identity in 128 aa)" FT /gene="SPAC11G7.04" FT /gene="ubi1" FT /product="ubiquitin family protein" FT /protein_id="CAB16209.1" FT /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF FT AGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLP FT PRATNCRKKKCGHTNQLRPKKKLK" FT misc_feature 10722..10799 FT /note="PS00299 Ubiquitin family signature" FT misc_feature 10872..11027 FT /note="Match to PF01020 Ribosomal_L40e, Ribosomal L40e FT family Score 135.98" FT CDS complement(11468..12424) FT /db_xref="GOA:O13698" FT /db_xref="SPTREMBL:O13698" FT /label=SPAC11G7.05c FT /note="SPAC11G7.05c, len:318, SIMILARITY:Bacillus FT subtilis., FABD_BACSU, malonyl coa-acyl carrier protein FT transacylase, (318 aa), fasta scores: opt: 334, FT E():1.1e-14, (31.8% identity in 308 aa)" FT /gene="SPAC11G7.05c" FT /product="putative malonyl coa-acyl carrier protein FT transacylase (EC 2.3.1.39)" FT /protein_id="CAB16210.1" FT /translation="MSAILFPGQGVDWKTWMQPYLENNIVQNTLKEAENVTEIEIRKYI FT VEAEAKSNLRQPITTIAQPAILACSIALLRAFPPFTKKFRFYVGHSLGEYSAFVASQTL FT SFSSALKLVQARAKAMSYASALCQNPTSMLAITLTSRFPTDNFLNTVYSAVQKYRLIDI FT ANVNSDRQIVLSGDKKELESITSTLSELVRSLGKLRSNWLDVSGAFHSRYMLPARDSLK FT NALGETEFNISPELCYTDSGKRFLPIISNVTAELYPADEEDIRRQLLLQCFRPVLFKNC FT LKTVKSKYGANLFYAYGPGTTMQSIAKQNGISTKSRP" FT misc_feature complement(11471..12421) FT /note="Pfam match to entry PF00698 Acyl_transf, Acyl FT transferase domain," FT CDS complement(13577..14869) FT /db_xref="SPTREMBL:O13699" FT /label=SPAC11G7.06c FT /note="SPAC11G7.06c, len:430, FT SIMILARITY:Schizosaccharomyces pombe, O42654, hypothetical FT 49.4 kd protein c10f6.15 in chromosome i., (432 aa), fasta FT scores: opt: 508, E():6.8e-28, (28.1% identity in 377 aa)" FT /gene="SPAC11G7.06c" FT /product="hypothetical protein" FT /protein_id="CAB16211.1" FT /translation="MLSFGGSLLSHSDIASRNKFSIDYSQFKAFFPGNWKFSNSKDKKP FT VRSPTLDEPVGDVDIDIFDVSKFYPLKRLSSIPEKIRANYHEELQTTSYDLVRNPFTHG FT LFCVVFYHQNKSYLKMGVTDNFNAFEEDSRVYQYGVNHKLSCKNLLLAINYSPKTYTKI FT FDFLRNGRAPLQVLVHHCMLYNFYPEDFQDALWCAVQAHVMNQVRLGRATLLHARACYQ FT KIGDIRMYLIDPHDLYFSPNSLKWLIICSKQFQALVHLEVKMDTLKIFRRRSKYFNLAR FT SCVSGFELSWLIMMTSIGSNASAVPVHAYLASKRILYPSIIPEEIFFMRKFDSSLFKDI FT KNIHELLGFLGRLFMDLQDCEKFHHDYTEYHCYRIGKPLYQNYEEEALRKKSQGVKDAD FT FIKPLVTNTRTAEKYKAFFEYKRNIVLKKQS" XX SQ Sequence 15885 BP; 4743 A; 3032 C; 2900 G; 5210 T; 0 other; ccgttgcaat gactgtaact cacgttgtga tacgaaatac catttcttgg gtcataaatg 60 taattcatgt cattcttaca atacttgtat ttcatcaatt tacaaacctt tggatcaccc 120 acaggtgtct atccctcgac tggcaaccgc tgaggatgcc ggtatgcgac ggctaatggg 180 acattcttgg gacaacagcg atgaagactt taatattttc gggattcaca gttttatata 240 gattttctaa ctttatgaac ttcctaaact gttacgatta tcctatatgt actattattt 300 tggaaaagtt gctattttca ttcaattctt tgcaagaatt acgaattttt aacgtttacc 360 ccaattgtct gattatattt attccctttt ctcgtccttt tgcatctact tcttatatgg 420 ttttttttct caacaagtca agcgatggta tgaagtaaat cgcttaaatt tcctcgatga 480 agagaatttc tgttttaatt aatctaaact catctacttt tgtgtgaagc tatggtgtct 540 tgaaacattc gcaaacataa ggactttgct tcatgcaatt ctgggcctct ctcaatattt 600 caatttcctg ttattatgac ttatatgtta tttgtcttga ttttttttac ggctggcatt 660 cattaaataa agagtttatt gtcattccat attcatctaa tccttcagat tccttttaaa 720 ctgacttttt gtctagaaga tgcgatcatt cgtcaagtta ctgcaactat tcttgttact 780 tttctatctg tcgttgtgat tctttagcag catccaacta caaaattttt ttaccacact 840 tgcagttgtc tcttaagagc aggtttagta acgtggatgg gttgttagtc ggagttacta 900 gtcagatgaa gcgataatac taattttcta ctatatcaat ttaatttggc atgaataaaa 960 tttcaataca ttgttactat gtaaggtggt taactcaatt accgcgtgga aacgatagtg 1020 tcaaatgcag ccatatgttt caagccagta gtaaatggca tctactgttg cggatgttta 1080 gtaatttaaa attagggaaa tctatatgaa tattgcagta tgtgaaaact gtggtagctt 1140 tctcattaag cgcaagttaa cggcgatctc tttttatcca gccatttgaa atgaataatt 1200 tgccttagta aaatttcacc tggttcgtaa ctttaaggtt ctttgctaga gcattgatag 1260 gtaacatatt ggtaaggtga aaaaaagcct cgaaactaac tcggaaagaa tccccacatt 1320 gaaaagtgca tcacccttac ctataggcaa agccaagcag gaccgcgaca ttttctcttt 1380 accgcttact cttcaccgtg cttttaaggg aattcatcaa gatacattga aattggacga 1440 gatcttatta tttttctaga aaaaaaatcc aacgttcttt taaaaacttc aaaaaacctt 1500 ctttctgtaa gggaattcgc acgcaagaga aaatgtcatt cacgaacaat acttcaagtg 1560 tggacacttc cctcagctca tctgcttcta gcagtattcc ggcaagcagc tcttctgctg 1620 ctgctagtac gagtttgagt agctcaagcg tgattccttc tagttcgagc tctatgcttt 1680 catcgtctag tgctactgca attagttctt catcatcgtc ttcacctctt tcatcctcat 1740 cttttacttc tcctgcttct tcttctttta tcacatcttt ggtctcatca agcagccaac 1800 aaagctctag tagcagtgct tcattgacct ctagtagcag tgctacactg acttccagta 1860 gcagtgcttc cccgacctcc agtagttcga gtcacgcgct tagcagcagc agtagttctc 1920 ttgtcgctag tagttcctcc agtgggatgt ccagtagttc cttaagccat tcgtctagtg 1980 tcccatcatc tagttcttcc taccacagct cttccatgac tacgagcggc ttgagttctt 2040 cagccagcat tgtcagtagt acgtatcgag atggaccctc aataatcaca ttggtctcaa 2100 cttcttacgt ttctgaagtg gtgaccccca ctactactaa taattggaat tcctccagtt 2160 ctttcacttc cagcacttcg tctacgccta ttagctcttc ttattcttcc agtggcactt 2220 tgcccagtaa atctaacaag tcaagcaatc atgtcggcgt agttgttggt tgttctgttg 2280 ctattcctgt tggtgtagtt ctaattttga ttggtttagg aatttttctt tggaaaaggc 2340 atcaacggtc caaacgaatt aaggctgagc gcatgcaaga agtcgaggaa tacggattta 2400 accccaatca accctccaat ttccgtagcc caaatcgtgc tccctctact aataacaggt 2460 atcgtggctg gaatggaagc ccaacacctg ctgctggaaa caatactaac ggacggcctg 2520 ttgctcctag accttctgcc ggcgctggcg gagccaatcc gcctgcagct tctcagcccg 2580 gccttttggg tggcagtagt aattcagctg gtccaattgc agctgctacc gctgctggtg 2640 tcggtgctga tgcttctgat gcagctaata ctggtggaag ctttactcgt cctcaaggag 2700 cccgtatggt tagaccgatt ggaaatccac ctgatctttc tgcttctaac gaagctgaag 2760 ctacaatgcc tccatctaat ggaagcaact tttctgaagg tttgtctgca tctccgtttg 2820 agtcaggccc tgctgttggt gccgctggag ctgcggccga agctgcagaa cacagtggtt 2880 ctggttcaga ttcctatcca gaaggtcctt tagctacgat tcctgaaagt gacagtgaaa 2940 gcatggctag tgatttagct ggagaatcct cttacggctc gagggcagct ctgtctagtc 3000 gatctcaaag caatcttctt tccccaacaa gcactggtgc ttctaaccag cccaattatt 3060 caccttttgc tgacaaccca tcaagtagca acgtttctat tcctcggtcg tcatcggaag 3120 ctcgcaggtt gaacctgttt taaagtatgc gcttaaacac ttgttgcgat agatttaagc 3180 aaaaattatt tgtttacaat ttctggatat atccgtcgct taaaataaat gcgttagtta 3240 cgcttgcctt caattgatgg tagaaagtct tgcgaagaaa agcttgctca tcactttttc 3300 gtataacaac tttttcttta ttcggctttt acctttgttt tttacgaaaa tcttaagtaa 3360 tcgatctttt caatcttatt gctatctctt tgttttaccg ttactcactc atttcctttt 3420 tgcttgaaca gttttatggt attagaaatt cacattctca ttgcaaagct ttgttattat 3480 tatgggtttc gcttatacca tctcatacaa tcaattggtc cgtcggttta ctttctttaa 3540 aaaaggagtt atttagcaaa ctatttatcc ttctttcaaa ctttcttttt aaacttaact 3600 atttgctttt tctcatggcc ctcttttttt aagaaaattt cgttgcttgc atttcattca 3660 aagtcttaaa tatgtcatct ggttccttaa cttattgctt ttaaatcgac ttcatctttt 3720 gactgacttt tgaatttttc tttcaattgt ttcactgtag tgagatacaa cccttaaaaa 3780 agtctctttg gtttgtttat tcttatcctt ttctcatttg catttcatta cttatcaatc 3840 atttctgttt cattcgccgt ttcgctttgt tttttcattt gtacatttct ttacagaatg 3900 gcgaattctt gtcaaatgcg acggaaagcc aattttcctg aatagtgtaa aacgaaatga 3960 aacttttaac agctacctgt tttccatccc atggtttctt gttattgaaa gtggatagca 4020 aatagtcttt atgaccagcc atttcaattt tactttcagt cgcctaacca atctcgttat 4080 ctcatttctt tgaaataaat gtacatactg tactttttcc ttttcttttc cttccgttcc 4140 tttttcaccg tctcgtatat gtaatggagc gtcacgttcc cttcgctgca cttatcgctc 4200 ttactctttc gtcatgttga ggaccatcta gctctacttt ctctcgtgtg gctggatgtt 4260 tacttaactt tgttttggcc agcatgctcg actactgata attaaaaaat aaaaattttg 4320 agcattgtct gtttaagagt tgttgctaag ttctggcttt attactttta ttcgcggcac 4380 tccgtttgcc ttcttgtatg taccttttac aattcttcgt ttcctctctt tcttctcagt 4440 tctaactatc acttgaacgc aaagtggtta gatatacatt ttcctcattc tttcgtatta 4500 aatcaattta ttaaatgctt atagcttgca aatcatacat tgtgctgcat gatatttact 4560 ttcgtagtta acacgtaaaa cgtattttct gtacgccaaa aaagataaag aacaatttgg 4620 taatcgcgtt gtatatatat aaggaaatgg gttataatgc gttaaggatc taatctgcaa 4680 gaccgattaa ttctcctttc agccaacacc acgcgaaatc cgacggattt caaaattgtg 4740 cgctgaaagc acgaatttga ttaaggattc cttttgtagt tcttttttat ttatttcgtt 4800 cccgaaaaaa gcgtttttgt atcaaataga aaggataaaa cggccttaat tgaaagtttt 4860 ttttgcaagc acaaatactt gactttcttt gggaaattgg ctttttttta ttatccaaaa 4920 gcaacgctgg tttgaaattt taccatcttt tccccctatg tcaaactcag ctcagtaagt 4980 agttctttaa ctgctataaa cacatttgct aacttttatg attagatctc gtcgaattcg 5040 agtaacaagt atgtgatgac attattcaaa tgccctgtgc aaacgctgac ccaaaatagt 5100 tgttgctgct gatggccttt acaaacgaga tgtgtttcgt tttcctgacc cgtttgcggt 5160 actaacagtg gatggtgagc aaacacatac cacaactgct atcaaaaaga cgttaaaccc 5220 ttattggaat gagacttttg aagtgtaagt gtatatccca ggttttgacc tttccaagta 5280 acttttatgt atatcaattt tagttggggt ttcttccagt ttatccctta taatttagct 5340 aatgttaact ttttgattaa aaacaatgtt tctaacaatt ctttagtaat gttacagata 5400 atagcaccat tgcgattcag gtgttcgatc agaaaaaatt taagaaaaaa ggccaaggct 5460 ttctaggagt gataaatctt cgtgttggag atgtgctcga tctcgccatt ggaggtgatg 5520 aaatgttgac ccgcgatttg aaaaagtcaa atgagaatac agtagttcat ggcaagataa 5580 tcataaactt atcaacgact gcgcagtcaa ccttacaggt tccatccagt gcagcatcag 5640 gtgcacgtac ccaacgtact agcattacca atgacccgca aagctcacaa agtagcagtg 5700 tgtctcgtaa ccctgcttcc tctcgtgctg gttcacctac ccgcgacaac gcacctgctg 5760 cttcccctgc tagttcagaa cctcgcacat ttagttcatt tgaagatcaa tatgggcgcc 5820 ttcctcctgg atgggaaaga cgtaccgata atcttggccg aacttattat gtagatcata 5880 atacaagaag cacaacgtgg attcgcccca acctaagttc tgttgccgga gcagccgcag 5940 ccgaattgca tagtagtgca tcgtctgcga atgttaccga aggtgttcaa ccttcctcta 6000 gcaatgcagc tcgtcgtaca gaagctagtg ttttgacctc taacgctact actgctggtt 6060 caggagagct tccaccggga tgggagcaaa ggtatacacc agagggtcga ccctactttg 6120 tggatcataa tacgcgaact actacttggg tggatccgcg ccgtcaacag tacattcgtt 6180 cctatggcgg tcctaataat gctactattc agcaacaacc tgtctctcaa cttggtcctt 6240 tgccaagtgg ttgggaaatg cgtcttacca atactgctcg tgtatatttt gttgatcaca 6300 ataccaagac taccacttgg gatgatcctc gtttaccttc gtcgttagat caaaacgttc 6360 ctcaatacaa acgtgatttc cgtagaaagt tgatttattt cctttcgcaa ccagctttgc 6420 atcctttgcc agggcagtgc cacattaaag tgcgtagaaa tcatatcttt gaagattcgt 6480 atgcggaaat tatgagacaa tctgcaaccg atttgaaaaa acgtttgatg attaagtttg 6540 atggtgaaga tggtttggat tacggtggat tatcccgtga atacttcttt ttgttatcac 6600 atgaaatgtt caaccccttt tattgtttat ttgaatactc ttcggttgat aattatacgc 6660 ttcaaattaa tcctcattct ggcattaatc cagagcattt gaactatttc aagttcatag 6720 gccgagtcat tggtctcgca attttccatc gtcggtttgt tgatgccttt ttcgttgttt 6780 ctttttacaa aatgatttta caaaagaagg tgacgttaca ggatatggaa agtatggatg 6840 cagagtatta tcgaagttta gtctggattt tggacaacga tataaccggt gttcttgatt 6900 tgacctttag tgtcgaagac aattgttttg gcgaggttgt tacgattgat ttgaagccga 6960 atggtcgaaa cattgaagtt acagaagaga ataaacgcga atatgttgat ttggtgactg 7020 tatggcgcat tcaaaaacgt atagaagagc agtttaatgc atttcatgaa ggttttagtg 7080 agctcatacc acaggaactg attaacgtgt ttgacgagag agaattggag ttgttgattg 7140 gaggcatttc cgaaattgac atggaggatt ggaagaagca tacggattat cgttcataca 7200 gtgaaaatga ccagattatt aaatggtttt gggaacttat ggatgaatgg agtaatgaaa 7260 agaaatccag acttttacaa tttaccactg gtactagccg aattcctgtc aacgggttca 7320 aagatttgca aggaagtgat ggcccccgta agtttactat tgaaaaagct ggtgaaccca 7380 ataaacttcc caaggcccac acctgtttca atcgacttga tcttcctcct tatacttcga 7440 aaaaagattt ggatcataaa ttgtccatag ctgttgaaga gacgattggt tttggtcagg 7500 agtaaaatgg atagctagct attgattact tttgatattt gaactattgg tgtttaacag 7560 tgaaaaagaa tttctgtgta aagtttccga aattattttt tttttctcat ttgagtgaaa 7620 atacttggat catcatgttc tacctttgtg ttctctacta ccattttcct tcttcttttt 7680 atacttgttt gcaaacacat ttcctcttaa tgctcttcgc acaaaacata taagttaatt 7740 tactattatt aagttacgta ctgcataagt gattttatat ttatgaaatt accgcccttt 7800 ttcaacattt tatttgaaac tgtgagtaaa taggtagtta gttggtaagg tttttgtatc 7860 ctgtccaggt cgttgcttat gtggtagtta cctggtcacc gtagtaaata gatgacatga 7920 aaagtttacc cacaactata ggctttattg aatgaaaagg gcttattaag aaaacaatag 7980 ccgcttcaat acatgtgtaa tcagaccgca gagctatgac agtgaagttg tgtttattat 8040 cgtaaacatt tatcatttgt tagtaataag ttgaatgctt atacattggt caaaattcag 8100 taatatcgaa ttcgataact atgaaatctc gtgcaaatat ttattttttg gatgtgtgaa 8160 cctatagtaa atagtataaa ttcttatagc gagttaaaaa acagtataaa ccttgatatt 8220 agagtagaag gtgaaatatc gtgatacaac tacaaaaagt attcttttaa agaggttaaa 8280 atgtcattgg ataaggtaac agaataaggt tcgtaaattg ctacctttaa ggagaattgg 8340 gcagtggggt aatcgatgga tgtgtaggta tttgtttggg ggtctcatcg gctttccttt 8400 accaaccaca atagccttgt aaaacaatcc tgtttgcatg ttacagaaaa gaagtaaaca 8460 agatacataa tgttcaaatc actagtaaga aaatcctcag cttttcaacc attaaagtat 8520 ggaggtaaat acacggtaac gttgattcca ggcgatggaa ttggacgaga aacgagcaat 8580 gcagtgacag agatctttaa aacggcaaac gttcctatcg aatttgaaga aattgatgtt 8640 acaggaatgg aaaaaaacaa taaatcgtct ggagacgcgc tacatgaggc aattcaatct 8700 ctcaagagaa ataaagtggg tttaaaggga attctgttca ctccatttga aaaaggaggc 8760 catacctcgt tcaatgttgc cttgagaaaa gaactcgata tctatgcctc tctagtgctc 8820 atcaaaaaca ttcctggatt taagactcgt catgacaatg ttgattttgc aattattcgt 8880 gaaaacactg aaggtgaata ctcgggtctc gagcaccagt cggtaccggg agtcgtggaa 8940 tctttgaaaa tcattacaga atacaaatca aagagaattg cacaatttgc ttttgatttt 9000 gccttacaaa atggccgcaa atcggtcact tgcatccaca aggctaacat catgaaattg 9060 gccgacggtc tctttcgaag gacgttttac gacgttgcta acggttacga cgctattact 9120 cctaaggatt taattgtcga caatgcctcc atgcaggctg tttctcgtcc ccagcaattc 9180 gatgttttag tgatgccgaa cttgtacggt agcattctta gcaacattgg aagtgctttg 9240 gtcggtggcc ccggtgtcat tccaggtgcc aattttggtc gtgactatgc tctattcgag 9300 cctggttgcc gtcatgttgg tttaagtatt actggacgtg gggaagccaa tcctacagct 9360 gccatcctta gcgcttgctt gatgcttcgt catttaggcc taaaagatta tgctgatctt 9420 atcaatgccg ctacttattc tgtcattgaa gagggtaaaa ccctgaccaa agacttgggt 9480 ggttcggctt ccactggtga tttcacgcat gccattttag aaagaatgga aagcttataa 9540 tttaattatt ccccccttcc ttttaaatga tataccctcc tgttaatgag tgtaatgaaa 9600 gctattgaag tcttaggctt ttagccgtgt caactacaat tttgacgctc atgttactac 9660 tccttttctc taagtaagtt gaaacgtttc ggaagctttt tttgatattt acgtcttcta 9720 tcagttatga taacacctct tccgtcggca aggaactcct gcctgacaag aagatgatga 9780 atttttctta cgtacgttcc gaatatgatt gatattttaa tttcattttt gtgtggattt 9840 tccatacttt gtagtgtctg gtttgttcgt ccttattact ttgtatttga tgtaaattat 9900 ggaattctac tttttatagc ataaatgcct gattagtcaa ttttggaacg aaaaaggttt 9960 gataatttcc attgtgcctc ctctaataat cacaattgct agatacgtcg attttccaga 10020 aagatagagg atggtaatta gtttcctaaa acccaagttt tatttttaaa atttagttat 10080 gaatactctt aaagaataat ttaataatct ttaacaggat ttagattaac atagtggttc 10140 acagtctatt catgtagatt tacacattca tctatctatg tcgataacag caatgcacag 10200 cccttaaatg tgaaagatgt gtcttttaaa gtagtaaaag gttagttcaa acacattact 10260 taacttatct ccaaccttcg aagtcattta tattccagtt gtgtaagcga cctttgcaac 10320 tatcgtcgtt cttaggttca actaacaata tgcgcaacat ttctatatag gctaatcgaa 10380 ggatgccggc tcctcctaat gcctatcaat atatccaaaa attaggagac catttatcgt 10440 caaaaatttc tcaacaatag ccatatcttc taccagacta caaaatatac catagtattt 10500 tactatgcgt tataatattt tactgaattt gtgctaagaa attaatgtcc accgctagag 10560 gacgcactaa acgtgactgc ttttttcggt aagggctctt acggtgcttt cagtgaagaa 10620 ggaatccttt gtccaccaca atcatgcaga ttttcgtcaa gactttgacc ggaaagacta 10680 tcacccttga ggtggagtcc tctgacacta ttgacaatgt caagagcaaa atccaagaca 10740 aggaaggaat tcctcctgac caacaacgtc ttatcttcgc tggaaagcaa ttagaggatg 10800 gccgtactct ttctgactac aacattcaaa aggagtctac ccttcattta gttctccgtc 10860 ttcgtggtgg tatcattgag ccctctctaa aggctttggc ttccaaatac aactgcgaaa 10920 agcaaatctg ccgtaagtgc tatgcccgtc ttcctccccg tgctaccaac tgccgcaaga 10980 agaagtgtgg tcacaccaac caacttcgtc ccaagaagaa gctcaaatag attatttctt 11040 ttggggtatt tcgtgttctt gttatttctg tcttggtaac tttacagata aagaaatatt 11100 aagaatattt tggattacac ggatgtaaac atgtgaaatt cactgtagtg tagcaaaatt 11160 cgagcaataa aaaatacttg tcttaaaact gtatcctttg taagaatgtg gagctattgt 11220 agtgtggata gtatttgttg ttaacgatta cagtatcatt taaattgatg gtgatgcatt 11280 tgattgtgca tagctttaaa atgttatgtt agacgatatg cattcctgta attattctaa 11340 ctcttgtcag aatgccaaaa gatcatgtat acaaatgagc aacatcatga ctgactaggt 11400 aaaaggaaaa aaattaatgg aattacagta gaaaggctaa tttttaacta ctcgcttcga 11460 aaagtcctca tggacgagac ttggtggaaa ttccattctg ctttgctatt gattgcatcg 11520 tagtcccagg accatatgca taaaataagt tggctccata tttgcttttg actgttttga 11580 ggcaattttt aaataaaaca ggacgaaaac actgtagcaa taattgtctc cttatatcct 11640 cttcatcagc tggataaagc tcagctgtga cattagatat gatgggtaaa aagcgcttac 11700 cagaatccgt ataacagagt tctggagaaa tattaaattc tgtttccccc aatgcatttt 11760 ttaaagaatc acgtgctgga agcatgtatc ttgaatgaaa tgcccccgat acatcaagcc 11820 aattcgatcg aagctttccc aatgaacgca ctaactctga gagtgttgaa gtaattgatt 11880 caagttcttt cttatcgcca gacagcacta tttgtctgtc agaatttaca ttggctatat 11940 caattaatcg atacttctga actgccgagt aaacagtgtt tagaaagttg tcagtcggaa 12000 atctggaagt taaagttatc gctaacatag aagtcggatt ttggcataaa gcagaagcat 12060 aactcattgc ttttgctctt gcttgaacta gttttagagc cgacgaaaat gaaagcgttt 12120 gactagctac aaaggcagaa tattctccca aagaatgtcc aacatagaac ctaaatttct 12180 ttgtgaaagg aggaaaggca cgaagcaatg caatactgca ggctaatatg gcaggttgtg 12240 caatcgtggt tataggctgc ctaagattag atttggcctc tgcctcaacg atatactttc 12300 gaatttcaat ttcagtcaca ttttctgctt cctttaaagt attttgaacg atattatttt 12360 ctaaatatgg ctgcatccat gttttccaat ccacaccttg gccaggaaac aggatagcag 12420 acattctctt ttaagtaata gggtaacaga gcatcaaaat tttgaaagga atactatttc 12480 aaatcaatgg agctgaaatt cgaagacaaa tcaaatttct gtaaaaacgt agaaagtttg 12540 acaatgagaa ctttagatct tcttgtttac acctgcgaca ccctctaata ttgtgtcgaa 12600 agaaatgctt gtaattgaat tttaaggaat ctagcacata cagtaattta aacaaagtga 12660 ttattgttaa tacttcttag tacttgctta gtgaaagtgg tttgaaatcc caattcaagc 12720 tcaatataac gaacgctggg tgtttttgta aatcaaccca tagacaataa tgacgaaagt 12780 taggtagaaa actgcaattg aaagtccttg atcggacaga caaaaatttg tgattgcttg 12840 aacgtatagg tagttgataa aattgaactc caccatgttc cacatgcagt tgtttaccac 12900 acattcagta accagaactg cgatttgtat cactttgtag acttacacca tacgaaatgt 12960 agacttcaag agtcaaaata aaatactatg aaatacctta gcaggttaat tagtatgaaa 13020 ttatcaatgg agaaataacg gcaactaata tatgagttgt ctataaaatg tctgatcatt 13080 aaagttactg acacttttgt tacgggtaga cagcaaggat ttggaataca ttacactatc 13140 ggcaatcaca aattgattgc ttaaattacg caagttaaga tgaaacagat tcaaacttca 13200 attttttata ctattccgtt taatgggact gttaattgaa aagatatgct ttagtaaatt 13260 attttctcca aataggaatg atacagcaaa gcagactatt ttactcttaa tagagatacg 13320 tccaatgtag cacaagaaga ttccattcga gtaggataag aaattaaatg cttggaagcc 13380 gaggttattc aattctaata aaatgaaatg caaattttac agcagatcac tattcattaa 13440 actttaggtg taacgggatc agcttgcttc tagtagagaa aaacaaatgg tggaaattac 13500 tatgaataaa tagggaaaaa atctagaaac ctataacagg tcatcgtatg gattaattga 13560 cttcataagg tgtcggtcat gattgctttt tcaagacaat attccttttg tattcaaaaa 13620 atgctttata cttctcagca gttctagtat tagtgaccaa tggttttata aagtcagcgt 13680 ctttaactcc ttgagatttc ttcctcaagg cctcctcctc atagttttga taaagcggtt 13740 tcccaattct gtaacaatgg tattcagtat agtcatgatg gaatttttcg caatcctgaa 13800 ggtccataaa caatcgccca agaaatccca aaagttcgtg gatattctta atatccttaa 13860 ataaggaaga atcaaatttc ctcataaaga aaatttcctc cggaataata ctaggataca 13920 atattcgctt agatgctaaa tatgcatgaa ccgggacagc tgaagcgttg gatccaatac 13980 tggtcatcat gattaaccaa cttaattcga aaccagacac acaggatctg gccaaattaa 14040 aatacttcga tctccttcga aagattttta aagtatccat tttaacttct aaatgaacca 14100 atgcctgaaa ctgtttagaa catattatta gccattttaa agaattgggg gaaaagtaca 14160 aatcatgtgg gtcaataagg tacatccgaa tatctccaat cttttgataa caagcacgag 14220 catgtaacaa agtagcgcgg ccaaggcgta cttgattcat cacatgtgct tgcacagcac 14280 accaaagagc gtcttgaaaa tcttctggat aaaaattata aagcatacag tggtgaacta 14340 acacttgtaa aggagcacgg ccgtttcgta aaaagtcgaa aattttagta taagtttttg 14400 gggaataatt tatagctaaa aggagatttt tgcaagaaag cttatgattt actccatact 14460 gataaaccct tgaatcttct tcaaaggcgt tgaagttatc ggtcacaccc atctttaagt 14520 aagatttgtt ttgatggtaa aagacaacgc aaaatagtcc atgcgtaaag gggtttcgta 14580 caaggtcata agaagtggtc tgtaattcct catgataatt agctctgatt ttttcaggaa 14640 tactgctaag gcgctttaag ggataaaatt tactaacatc aaaaatgtca atatccacat 14700 ctcccactgg ctcgtccaac gtaggggacc tcaccggttt cttgtctttg gaattactaa 14760 acttccagtt tcccggaaaa aatgctttaa attgtgaata atctatagag aatttattcc 14820 gggatgcaat atcagaatgt gacaacaaac tcccaccgaa ggaaagcata ttcgtatcgt 14880 atgttggaaa tttcgctatc agctttctta aaaggatgac agtcgaataa ttgtgcttct 14940 tcaaatatca ctcctttttc gcaaaatcta agaacccttt caaaatctaa gggcatgaat 15000 taggtcaact taaaccaaaa agcgaaatga actgtgagga ataatacttg aagaagagga 15060 ttaagcgcct tgcatacaag actaaacaaa atagtatatt aaggcagagg agaagtaatg 15120 aattcaggga gaattataaa aaggctactt atctcgtata cctgacaaga aaaccaaaga 15180 taaccaccct tcagcgttag aatcaagggt ctaataaacg tcaagaatgt caatagtaaa 15240 cctctccttt tagtataaga aatgtgggaa tgggtaaaaa taaaaataaa aaagcaaaag 15300 ttatgacaag atggataaaa gaagagtttt cgagtttttg aaccagtata tacctcaaaa 15360 gtggatcaca cataggtgta aacaaacaat cttaacaagt caatttgagc aacatattat 15420 tctatctcaa aagcaattgg aaaacaatta aaaacaacaa cagattatta tcctatttgt 15480 gcattgcaat gtataaagca agttatgtga attttttcat acagcatcaa gtcctttctc 15540 aacgtcatgt tttaaatgtt tttgccctct aacacgaaaa aaaccattct aagtaagcaa 15600 agcttcaact agccgattcg tgtgtttgat ttctattatg ttcccactgt ctctactttg 15660 agacttctca tacacaagca gaaaaccaat cgattaccaa tcttagtttt ctttttccag 15720 cagaattcgt aaaaagttaa ttttacaata actgaattaa aaaattgcaa tatttctgtt 15780 aattaaatca gttcggctaa ctttaatctt ccataaagca agcagagctt aattgttaaa 15840 aatatatgta ttttatatga tgtgttccat aaaaaaacaa ggatc 15885 //